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Artykuły w czasopismach na temat "Lupinus Angustifolius"
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Bielski, Wojciech, Michał Książkiewicz, Denisa Šimoníková, Eva Hřibová, Karolina Susek i Barbara Naganowska. "The Puzzling Fate of a Lupin Chromosome Revealed by Reciprocal Oligo-FISH and BAC-FISH Mapping". Genes 11, nr 12 (10.12.2020): 1489. http://dx.doi.org/10.3390/genes11121489.
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Old World lupins constitute an interesting model for evolutionary research due to diversity in genome size and chromosome number, indicating evolutionary genome reorganization. It has been hypothesized that the polyploidization event which occurred in the common ancestor of the Fabaceae family was followed by a lineage-specific whole genome triplication (WGT) in the lupin clade, driving chromosome rearrangements. In this study, chromosome-specific markers were used as probes for heterologous fluorescence in situ hybridization (FISH) to identify and characterize structural chromosome changes among the smooth-seeded (Lupinus angustifolius L., Lupinus cryptanthus Shuttlew., Lupinus micranthus Guss.) and rough-seeded (Lupinus cosentinii Guss. and Lupinus pilosus Murr.) lupin species. Comparative cytogenetic mapping was done using FISH with oligonucleotide probes and previously published chromosome-specific bacterial artificial chromosome (BAC) clones. Oligonucleotide probes were designed to cover both arms of chromosome Lang06 of the L. angustifolius reference genome separately. The chromosome was chosen for the in-depth study due to observed structural variability among wild lupin species revealed by BAC-FISH and supplemented by in silico mapping of recently released lupin genome assemblies. The results highlighted changes in synteny within the Lang06 region between the lupin species, including putative translocations, inversions, and/or non-allelic homologous recombination, which would have accompanied the evolution and speciation.
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Mauer, Oldřich, Dušan Vavříček i Eva Palátová. "Assessing the influence of the Lupinus genus in the biological reclamation of sites degraded by whole-area dozer soil treatment". Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 61, nr 3 (2013): 711–20. http://dx.doi.org/10.11118/actaun201361030711.
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The paper deals with possibilities of using the blue lupine (Lupinus angustifolius L.), white lupine (Lupinus albus L.) and garden lupine (Lupinus polyphyllus Lindl) in the biological reclamation of sites degraded by whole-area dozer soil treatment.The lupines were sown into strips or broadcast. The effect of lupines onto the growth and health condition of the young plantations of Norway spruce, European beech and Scots pine was studied together with their influence on the site soil characteristics. The experiment showed that the sowing of lupine favourably affected biometrical characteristics of newly planted trees. Even though the soil humus content did not increase in the experimental period of 5 years, the nitrogen nutrition as well as the nutrition with other biogenic elements improved and the symptoms of chlorosis were eliminated. In the conditions of the Krušné hory Mts., the lupines can produce up to 3.6 tons of biomass dry matter and favourably affect the nutrition of planted trees.
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Susek, Karolina, Wojciech Bielski, Katarzyna B. Czyż, Robert Hasterok, Scott A. Jackson, Bogdan Wolko i Barbara Naganowska. "Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution". Genes 10, nr 4 (1.04.2019): 259. http://dx.doi.org/10.3390/genes10040259.
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Plant genome evolution can be very complex and challenging to describe, even within a genus. Mechanisms that underlie genome variation are complex and can include whole-genome duplications, gene duplication and/or loss, and, importantly, multiple chromosomal rearrangements. Lupins (Lupinus) diverged from other legumes approximately 60 mya. In contrast to New World lupins, Old World lupins show high variability not only for chromosome numbers (2n = 32–52), but also for the basic chromosome number (x = 5–9, 13) and genome size. The evolutionary basis that underlies the karyotype evolution in lupins remains unknown, as it has so far been impossible to identify individual chromosomes. To shed light on chromosome changes and evolution, we used comparative chromosome mapping among 11 Old World lupins, with Lupinus angustifolius as the reference species. We applied set of L. angustifolius-derived bacterial artificial chromosome clones for fluorescence in situ hybridization. We demonstrate that chromosome variations in the species analyzed might have arisen from multiple changes in chromosome structure and number. We hypothesize about lupin karyotype evolution through polyploidy and subsequent aneuploidy. Additionally, we have established a cytogenomic map of L. angustifolius along with chromosome markers that can be used for related species to further improve comparative studies of crops and wild lupins.
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Święcicki, Wojciech, Katarzyna Czepiel, Paulina Wilczura, Paweł Barzyk, Zygmunt Kaczmarek i Magdalena Kroc. "Chromatographic Fingerprinting of the Old World Lupins Seed Alkaloids: A Supplemental Tool in Species Discrimination". Plants 8, nr 12 (27.11.2019): 548. http://dx.doi.org/10.3390/plants8120548.
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The total contents and qualitative compositions of alkaloids in seeds of 10 Old World lupin species (73 accessions) were surveyed using gas chromatography. The obtained results, combined with those for three lupin crops, Lupinus angustifolius, Lupinus albus, and Lupinus luteus, provide the most complete and up-to-date overview of alkaloid profiles of 13 lupin species originating from the Mediterranean Basin. The qualitative alkaloid compositions served as useful supplementary tools of species discrimination. On the basis of the most abundant major alkaloids, lupanine, lupinine, and multiflorine, the Old World lupin species were divided into four groups. Those containing lupanine (L. angustifolius, L. albus, and Lupinus mariae-josephi), containing lupinine (Lupinus luteus, Lupinus hispanicus, and Lupinus × hispanicoluteus), containing lupinine and multiflorine (Lupinus atlanticus, Lupinus palaestinus, Lupinus anatolicus, Lupinus digitatus, Lupinus pilosus, and Lupinus cosentinii), and containing multiflorine (Lupinus micranthus). Within a given group, certain species can be, in most cases, further distinguished by the presence of other major alkaloids. The discrimination of species based on the total alkaloid content was found to be less reliable because of the significant intra-species variations, as well as the influences of environmental factors on the seed alkaloid content.
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Plessner, O., A. Dovrat i Y. Chen. "Tolerance to iron deficiency of lupins grown on calcareous soils". Australian Journal of Agricultural Research 43, nr 5 (1992): 1187. http://dx.doi.org/10.1071/ar9921187.
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Lupins differ in their efficiency to utilize Fe3+ in soils containing CaCO3. Most lupin species are susceptible to Fe deficiency. The objective of this study was to screen different lupin species, including introduced cultivars and wild types collected in Israel, for susceptibility to Fe deficiency. In a greenhouse experiment, inoculated seedlings, 7 to 10 days old, were planted in 1 L pots filled with a mountain rendzina soil from Emek Haela (pH=7.3, CaCO3- 45%), or with a brown-red sandy-loam soil from Rehovot (pH=7.7) not containing CaCO3. On the calcareous soil, susceptible lupin plants from day 7 onward showed various degrees of chlorosis, suggesting Fe deficiency, which at a later stage caused reduction of growth, necrosis of the leaf tissue, leaf abscission and ultimately death of the plants. Soil application of FeEDDHA resulted in an immediate remedy of the deficiency symptoms. No deficiency symptoms were observed on plants growing on the sandy-loam soil lacking CaCO3. Ranking of lupins based on visual symptoms of Fe deficiency and on chlorophyll concentration of young leaves relative to their tolerance to soil lime was as follows (location of source plants in brackets). Very good: Lupinus pilosw (Emek Haela), L. cosentinii cv. Erregulla and L. angustifolfus cv. Yorrel. Moderate L. angustifolius cv. Illyarrie. Low L. palaestinus (Gesher Haziv), L. albus cv. L221, L. angustifolius (Gesher Haziv). Very low: L. angustifolius (Givat Brenner), L. palaestinus (Rehovot). The need for further exploration of wild lime-resistant genotypes is emphasized.
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Castillo, Rafael Fernández, Raquel García Pérez, Ana González Díaz i Antonio Liñán González. "Therapeutic Applications and Effects of Lupinus angustifolius (Blue Lupin) and Its Components: A Systematic Review and Meta-Analysis". Foods 12, nr 14 (19.07.2023): 2749. http://dx.doi.org/10.3390/foods12142749.
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Lupinus angustifolius has a unique nutrient profile among legumes and may have beneficial health effects when included in the diet. The aim of this study was to investigate the biological properties of blue lupin (Lupinus angustifolius), its chemical components, and their relevance for monitoring biological and anthropometric health markers, including triglycerides (TGs), low-density lipoprotein cholesterol (LDL-C), BMI, weight, and glycemia, compared with control groups with other kinds of diets. PubMed, Web of Science, and Scopus databases, updated to December 2023, were searched. Out of the 194 studies identified, a total of 7 randomized controlled trials (RCTs) comprising 302 participants met the eligibility criteria. The results of our study indicated that the blue lupin diet has a direct relationship with parameters such as blood glucose, weight, and LDL-C but not with TGs or BMI. In conclusion, the research described in this review clearly indicates that L. angustifolius may play an important role in the dietary prevention of hyperlipidemia and hypertension. Therefore, it would be highly advisable to increase its consumption in diets. However, further studies, ideally in humans, are required to truly establish L. angustifolius’s health-promoting properties.
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Miao, Z. H., J. A. Fortune i J. Gallagher. "The potential of two rough-seeded lupin species (Lupinus pilosus and L. atlanticus) as supplementary feed for sheep". Australian Journal of Agricultural Research 52, nr 6 (2001): 615. http://dx.doi.org/10.1071/ar99142.
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The rough-seeded lupins are better adapted to alkaline soils than the domesticated lupins currently in use in commercial agriculture in southern Australia. Lupinus pilosus and L. atlanticus are two species of rough-seeded lupins that are undergoing domestication, and could be very valuable for sheep as a supplementary feed. However, there is little information on the nutritive value of these lupins. Two experiments were conducted to evaluate the nutritive value of L. pilosus and L. atlanticus, compared with L. angustifolius, which is widely used as an animal feed in Australia. The results showed that the rough-seeded lupins examined had larger seeds, a greater proportion of seed coat in the whole seed, and a higher fibre content in the seed coat than domesticated lupins. Nitrogen (N) content in the kernel of the various lupin species was similar. The high fibre content in seed coat did not appear to limit the digestion of the seeds by sheep as demonstrated by the high potential degradability of seed dry matter (DM) for all lupin species. There were no significant differences betweenL. pilosus,L. atlanticus, andL. angustifolius as a supplementary feed provided at low levels in DM digestibility (DMD), apparent energy digestibility (AED), and N-balance, suggesting that L. pilosus and L. atlanticus could be used in place of L. angustifolius. AlsoL. atlanticus could be substituted for L. angustifolius at high levels of supplementation as there were no differences in DMD, AED, apparent N digestibility, and N-balance when these species were fed to sheep as a supplement to barley straw. Supplementation with lupin seed at 150 g/day significantly improved DM intake by 195 g/day, DMD by 8.7, and AED by 11.4 percentage units. However, a high level of lupin supplementation in a diet based on barley straw did not increase DMD and AED of the diet.
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Wink, Michael. "Site of Lupanine and Sparteine Biosynthesis in Intact Plants and in vitro Organ Cultures". Zeitschrift für Naturforschung C 42, nr 7-8 (1.08.1987): 868–72. http://dx.doi.org/10.1515/znc-1987-7-823.
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[14C]Cadaverine was applied to leaves of Lupinus polyphyllus, L. albas, L. angustifolius, L. perennis, L. mutabilis, L. pubescens, and L. hartwegii and it was preferentially incorporated into lupanine. In Lupinus arboreus sparteine was the main labelled alkaloid, in L. hispanicus it was lupinine. A pulse chase experiment with L. angustifolius and L. arboreus showed that the incorporation of cadaverine into lupanine and sparteine was transient with a maximum between 8 and 20 h. Only leaflets and chlorophyllous petioles showed active alkaloid biosynthesis, whereas no incorporation of cadaverine into lupanine was observed in roots. Using in vitro organ cultures of Lupinus polyphyllus, L. succulentus, L. subcarnosus, Cytisus scoparius and Laburnum anagyroides the inactivity of roots was confirmed. Therefore, the green aerial parts are the major site of alkaloid biosynthesis in lupins and in other legumes.
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Hamblin, J., R. Delane, A. Bishop i G. Adam. "The yield of wheat following lupins: effects of different lupin genotypes and management". Australian Journal of Agricultural Research 44, nr 4 (1993): 645. http://dx.doi.org/10.1071/ar9930645.
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More nitrogen is fixed by grain lupins (Lupinus sp.) than is removed in the harvested grain. Differences in residual N after different lupin species and genotypes (L. albus, L. cosentinii, L. angustifolius) and different agronomic treatments (harvesting, sowing dates, sowing rates and fertilizer treatments) were estimated using a simple nitrogen (N) balance. For six experiments, the relationship between the estimated residual N value and the yield of the following wheat crop was also examined. L. albus grew poorly on the infertile sandy soils and had the lowest estimated residual N value, whereas L. cosentinii had a high estimated residual N value and L. angustifolius was intermediate. Across sites and seasons, the estimated residual N value of L. angustifolius varied from 37 to 165 kg N/ha. For an individual experiment the greatest range for L. angustifolius was from 59 to 114 kg N/ha. Despite the wide range in residual N across genotypes, there was a poor relationship between estimated residual N value and yield of the following wheat crop. Except for lupin crops which grew poorly, the impact of agronomic treatment on the residual N from lupins had little effect on yield of the following wheat. On the basis of these results there seems to be no reason to manage the lupin crop for high residual N value, and any improvements to the nitrogen harvest index of lupins through breeding or management are not expected to reduce the residual effect.
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Marley, C. L., R. Fychan, V. J. Theobald, D. R. W. Davies, R. Sanderson, M. T. Abberton i D. R. Davies. "The effects of incorporating either lupins or soya bean meal into concentrate diets when compared with a control concentrate diet on the performance and carcase characteristics of finishing lambs". Proceedings of the British Society of Animal Science 2009 (kwiecień 2009): 52. http://dx.doi.org/10.1017/s175275620002891x.
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Soya bean (Glycine max) meal is an important component of animal feed and oil seed rape and palm kernel cake and meal are typically used in commercial concentrate diets for sheep in the UK.. Lupins (Lupinus; Leguminosae) as a high protein, high energy, nitrogen-fixing grain legume, have potential as a home-grown livestock feed in the UK (Wilkins and Jones, 2000). Research has been conducted on the effects of narrow-leafed lupins (Lupinus angustifolius) (Hill, 2005) but there have been few studies on the effects of yellow lupins (Lupinus luteus) when fed to sheep. This study investigated the effects of incorporating either yellow lupins, narrow-leaf lupins or soya bean meal into the concentrate diets of finishing lambs on lamb productivity and carcass characteristics when compared to a commercial UK lamb finisher diet.
Rozprawy doktorskie na temat "Lupinus Angustifolius"
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Allardice, Roderick. "Does the application of vermicompost solid and liquid extracts influence the growth, N-nutrition and soil microbial diversity of the legume, Lupinus angustifolius?" Thesis, Stellenbosch : Stellenbosch University, 2015. http://hdl.handle.net/10019.1/98107.
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Thesis (MSc)--Stellenbosch University, 2015.ENGLISH ABSTRACT: Vermicomposts (VCs) are the solid excreta of earthworms, known to contain plant available nutrients, large amounts of microbial life and diversity, and plant growth regulating hormones. VCs may play an integral role in the nitrogen nutrition of Lupinus angustifolius and function to reduce the reliance of legume crops on chemical fertilizers. Furthermore, the effects of the combination of VC solids and VC teas on legume growth and N nutrition, is unknown. The aim of the present study was to determine the role of varying concentrations of chicken manure VC, with and without the additions of varying VC tea concentrations, on the substrate bacterial functional diversity, plant biomass and N nutrition of the legume, L. angustifolius. In the first experiment the plants were grown in pots under glasshouse conditions and VC was substituted into the quartz sand growth media at rates of 5%, 10% and 100%. Furthermore, rhizobia inoculated and non-inoculated groups were established within the VC treatments. The plants were harvested after 30 days and analysed for tissue nutrient concentrations and biomass production. The VC-containing substrates were assessed for wide-spectrum soil analyses, nematode diversity and microbial diversity via Biolog EcoPlates. In the second experiment the plants were grown under similar conditions for 50 days and amended with 5% VC in the growth media as well as 50ml of aerated VC teas. The teas were brewed for 24 hours in concentrations of 4%, 10% and 20% (v/v), with molasses and kelp extract added as sugar sources. The combined treatments of 5% VC and rhizobia inoculation yielded the greatest biomass response. Furthermore the addition of VC allowed for bacterial nitrogen fixation within non-rhizobia treatments. However, changes in VC concentration had no effect on bacterial guild structure but were found to effect nematode funtional diversity. The additions of VC teas to soil media containing 5% VC had no effect on biomass production but were found to influence bacterial nitrogen fixation. Lower concentration teas increased BNF while the 20% tea reduced this parameter significantly over 50 days. The 20% tea also contained significantly greater bacterial functional diversity than the 5% and 10% teas. The findings of study indicate that the combined treatment of VC solids and teas do not increase the plant biomass of L. angustifolius, but that the additions of 20% teas result in greater microbial diversity in the soil. This in turn may lead to increases in soil fertility. Furthermore, additions of high concentration vermicompost teas (20%) shift the dependence of the plant from atmospheric N sources to soil N sources.
AFRIKAANSE OPSOMMING: Geen opsomming beskikbaar
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Ratanasanobon, Kanokwan. "Investigation of alternative approaches to narrow-leafed lupin (Lupinus angustifolius) genetic transformation". Thesis, Ratanasanobon, Kanokwan (2014) Investigation of alternative approaches to narrow-leafed lupin (Lupinus angustifolius) genetic transformation. PhD thesis, Murdoch University, 2014. https://researchrepository.murdoch.edu.au/id/eprint/22974/.
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Narrow-leafed lupin (Lupinus angustifolius) is one of the top six crops that contribute value to the Australian economy. Gene transfer technology has been studied as a strategy to improve lupin varieties against diseases to improve yield, production and seed quality. However, the established method used for transformation of lupin is based on Agrobacterium and embryonic axes as explants is a method of low efficiency. The aim of this project was to investigate the alternative genetic transformation methods for genetic manipulation of narrow-leafed lupin (L. angustifolius) to improve the transformation efficiency. Two potential genetic transformation methods were investigated: particle bombardment (direct gene transfer), and in planta transformation (Agrobacterium-based transformation). In addition, lupin-Agrobacterium interactions were studied to provide information of the factors limiting transformation, and whether the involvement of an additional virG using construct carrying virGN54D (constitutive virG mutant carrying Asn-54 to Asp amino acid substitution) improved lupin transformation efficiency.
In this project, a genetic transformation protocol using particle bombardment for narrow-leafed lupin was accomplished. The following conditions were identified as being optimal for transformation via particle bombardment using a helium inflow particle gun with lupin embryonic axes as target explants:
a) Embryonic axes used as explants were pre-treated in MS media supplemented with 5 mg/L BAP and 0.5 mg/L NAA, 3% sucrose and 0.7% agar for 3 days in the dark at 25oC.
b) Pre-treated explants were placed onto MS media with 0.3 M mannitol as osmoticum 4 h prior to bombardment.
c) Bombardment was carried out by:
• A precipitation protocol using plasmid DNA prepared at 2 ng DNA per μg tungsten particles.
•Bombardment was carried out twice at 400 psi with a 7 cm target distance with 10 μL coated particles.
d) Bombarded explants were kept on osmoticum media (MS media with 0.3 M mannitol) for 4 h then transferred to pre-/post-treatment media for post-treatment for another 3 days and kept in the dark at 25oC.
e) After post-treatment, explants were transferred to selection media (MS medium with 1 mg/L BAP and 0.1 mg/L NAA, 3% sucrose, 0.7% agar and 10 mg/L PPT) for 8 weeks with subculture every two weeks. Surviving shoots were transferred to rooting media and analysed for presence of the transgene by PCR.
A transformation efficiency of 0.4% for T0 production was achieved as confirmed by amplification of a gus gene by PCR. However those transformed explants did not form roots.
In planta transformation of seedlings and flowers of narrow-leafed lupin was investigated. For seedling transformation, factors essential for delivering A. tumefaciens to the target tissues (L2 layer of apical meristem of seedings) and to enhance the ability of A. tumefaciens cells to transform plant cells were studied and optimised. Sonication and vacuum infiltration facilitated penetration of A. tumefaciens cells to the target tissue, sonicating seedlings 15 min before 10 min infiltration with A. tumefaciens cells gave the best overall balance of both gene transfer determined by GUS staining and seedling survival rate. The Agrobacterium induction condition and infiltration medium was developed after testing and optimising of media and Agrobacterium growth. Modified LB medium with glucose 30 g/L was the best medium that gave the highest percentage of shoots showing GUS expression, at 35±5% which was significantly higher than the control infiltration media used for Medicago and A. thaliana in planta transformation at the 0.05 level Tukey HSD. The combined optimised conditions were further tested. Some shoots, picked at random, were positive for GUS expression, including the whole apical area and parts of leaves of new shoots, indicating gene transfer and stable transformation although chimeric. However, transformants were not obtained. Further investigations suggested that there may not have been enough viable A. tumefaciens on seedling shoots for successful transformation. Survival of A. tumefaciens cells on the plant tissues was about 103 times less than routinely used for transformation of lupins in the established in vitro lupin transformation method.
In in planta transformation of lupin flowers, experiments were designed to deliver Agrobacterium cells to lupin ovules as target tissues. Factors reported to contribute to success in this type of transformation, such as using surfactant, infiltration period and times under vacuum and composition of infiltration medium were tested and optimised for lupins. Thirty plants with floral inflorescences having flowers ranging from the dome to open stages were infiltrated twice for 3 min each with MS liquid medium containing 10 mM glucose, 0.01% Silwet L-77 and A. tumefaciens cells in early exponential stage at concentration of OD 1.87. Pod set was 10.82 %. No transformant was obtained. The same infiltration media and conditions were used with Arabidopsis thaliana cv Columbia as control plants and transformants obtained at 0.255%. Histology studies of lupin flower structure by SEM and wax-embedded sectioning revealed that there did not appear to be a physical channel for A. tumefaciens cells to gain access to the ovule via the stigma or style before anthesis. Furthermore, Agrobacterium cells could not gain access to the ovule through the immature carpel of young flowers as the developing carpel closed while the ovule developed inside.
Interactions between Agrobacterium and lupin were studied to determine which stages limit transfer of genes from Agrobacterium to lupins, and which might be modified to achieve and/or improve transformation efficiency by A. tumefaciens in a genotype-independent fashion. The stages studied were: the attachment of Agrobacterium to the lupin explants, T-DNA transport across the cell wall and through the cell membrane. In addition, the effect of an extra virG was examined to find out if it would increase T-DNA transport. The interaction studies were done by comparing reactions to gene transfer in lupin cultivars Merrit and Quillinock which have significant difference in transformation efficiency (6.5% for cv Merrit and ~1% for cv Quillinock). The results of the attachment of Agrobacterium cells experiment showed no significance statistically in the number of bacterial cells attached to the explants (half embryonic axes) of six cultivars of narrow-leafed lupin (cvs Merrit, Quilinock, Belara, Illyarrie, Yorrel and Danja), indicative that the attachment stage was not the factor limiting gene transfer. T-DNA transport through the cell wall and cell membrane was evaluated through gus expression in experiment using cell suspensions (cells with cell walls) and protoplast (cells without cell walls) with T-DNA transfer determined by the relative intensities of the RT-PCR amplicons. The results showed, unexpectedly, that cv Merrit had less T-DNA transferred by A. tumefaciens than cv Quillinock in cell suspensions but not in protoplasts. The results were supported with MUG assays of transient expression of the gus reporter gene in cell suspensions of both cultivars. This indicated that the differences in cell wall composition between these two cultivars played an important role in gene transfer, but the factors limiting transformation success in Quillinock were downstream from T-DNA transfer to the cytoplasm of the host cell, possibly involving in T-DNA integration and/or expression, or selection and recovery of whole plants. The effect of an extra virG was examined with lupin, virGN54D increased transient expression of gus only in cv Quillinock cells, not in cv Merrit cells. Constructs carrying virGN54D may, therefore, be of some use as a component of a transformation protocol for cv Quillinock, and possibly other recalcitrant lupin cultivars.
This work has confirmed the relative difficulty of transforming narrow-leafed lupins, and it is concluded that despite investigating a series of alternative approaches, the method based on ‘stab inoculation’ of apical meristems, followed by selection of chimeric tissues to generate transgenic inflorescences, appears to be the most reliable approach. However, it is strongly genotype dependant, and improvements in efficiency and reduced genotype dependence are still desirable.
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Miao, Zhihong. "The influence of domestication and environment on the value of Lupins (Lupinus spp.) as a feed for ruminants". Title page, contents and summary only, 1998. http://web4.library.adelaide.edu.au/theses/09AFP/09afpm618.pdf.
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Bibliography: leaves 141-162. Lupin seed (L. angustifolius) is widely used in Australia as supplementary feed for ruminants. However, it does not perform as well on fine-textured and/or alkaline soils, unlike wild lupins. Field trials investigated the effect of domestication of L. angustifolius on seed structure and chemical composition for use as a benchmark for the breeding and selection of wild types L. atlanticus and L. pilasus. The effects of environemental factors on yield and chemical composition were also investigated. Investigation into the feed value of wild lupins indicated their potential as feed supplement for ruminants either by direct grazing or seed supplement.
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Geering, Andrew D. W. "The epidemiology of cucumber mosaic virus in narrow-leafed lupins (Lupinus angustifolius) in South Australia". Title page, table of contents and summary only, 1992. http://web4.library.adelaide.edu.au/theses/09PH/09phg298.pdf.
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Le, Roux Marcellous Remarque. "Phosphoenolpyruvate (PEP) metabolism in roots and nodules of Lupinus angustifolius under P stress". Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/50060.
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Thesis (MSc)--Stellenbosch University, 2004.ENGLISH ABSTRACT: This study investigated the activities of several of the enzymes involved in the alternative route of PEP metabolism via PEPc (EC 4.1.1.31). This reaction circumvents the adenyl ate-controlled PK (EC 2.7.1.40) reaction of the conventional glycolytic network under conditions of P stress. It was hypothesized that the synthesis of pyruvate under Pi stress would induce the PEPc alternative route and that C for pyruvate synthesis would primarily be imported via this route. This was assessed by looking at how total enzyme activities are perturbed under P stress and also by following the route of radioactive labelled 14C02 under sufficient (2 mM) and deficient P (2 JlM) conditions in either roots or nodules. The significance of the pathway under P stress, was further assessed by determining pool sizes of pyruvate that was synthesized from PEPc-derived C. The experiments were conducted under glasshouse conditions, as two separate studies: one to investigate the phenomenon of Pi stress and its consequences for PEPc-derived C metabolism, and the other one to study the enzymes involved. Seeds of Lupinus angustifolius (cv. Wonga) were inoculated with Rhizobium sp. (Lupinus) bacteria and grown in hydroponic culture. Tanks were supplied with either 2 JlM P04 (LP) or 2 mM P04 (control) and air containing 360 ppm CO2. Roots experienced pronounced P stress with a greater decline in Pi, compared to nodules. LP roots synthesized more pyruvate from malate than LP nodules, indicating the engagement of the PEPc route under Pi stress. In this regard, pyruvate is considered as a key metabolite under Pi stress. The role of pyruvate accumulation under Pi stress, was further highlighted by the metabolism of PEP via both the PK and PEPc routes. The enhanced PK activities supported these high pyruvate levels. Under P stress, PEPc activities increased in roots but not in nodules and these changes were not related to the expression of the enzyme. Root and nodular PEPc were not regulated by expression, but possibly by posttranslational control. The novelty of our results for symbiotic roots demonstrates that using metabolically available Pi is indeed a more sensitive indicator ofP stress. These results show that under Pi stress, nodules are able to maintain their Pi and adenylate levels, possibly at the expense of the root. It is suggested that nodules do not experience P stress to the same extent as roots or alternatively function optimally under conditions of low P availability. The increase in concentration of pyruvate synthesized from malate, indeed suggest that under LP conditions there is an increase requirement for pyruvate. It is clear from this data that the operation of bypass route in nodules should be investigated further. Nevertheless, this study provided incentives for understanding the role of C pathways in Ni-fixation, in particular under conditions ofP limitation.
AFRIKAANSE OPSOMMING: Die doel van hierdie studie was om die aktiwiteite van verskeie ensieme van die alternatiewe metaboliese roete via phosphoenolpirovaat karboksilase (pEPc, EC 4.1.1.31) te ondersoek. Dié reaksie omseil die adenilaat-beheerde pirovaatkinase (PK, EC 2.7.1.40) reaksie van die konvensionele glikolitiese weg onder toestande van fosfaat (P) stremming. Dit is gepostuleer dat die sintese van pirovaat onder toestande van Pstremming die alternatiewe roete via PEPc sou induseer en dat die koolstof (C) vir pirovaatsintese gevolglik hoofsaaklik vanaf hierdie roete sou kom. Dit is bepaal deur die veranderinge in die totale ensiemaktiwiteite wat sou plaasvind onder P-stremming te ondersoek. Daar is ook gekyk na die roete' wat radioaktiewe C C4C02) sou volg in wortles en wortelknoppies wat behandel is deur blootsteling aan eerder lae fosfaat (2 1lM) of genoegsame fosfaat (2 mM; kontrole), Die betekenis van die alternatiewe roete is ook ondersoek deur die poel-groottes van pirovaat, soos gesintetiseer via die PEPc reaksie, te bepaal. Twee eksperimente is in 'n glashuis uitgevoer. Eerstens is die verskynsel van Pstremming, asook die invloed daarvan op PEPc-afgeleide C-metabolisme, bepaal. Tweedens is die betrokke ensieme bestudeer. Sade van Lupinus angustifolius (cv. Wonga) is geïnokuleer met Rhizobium sp. (Lupinus) bakterieë en in 'n waterkultuur gekweek. Die houers is voorsien met óf2 IlM P04 (LP) óf 2 mM P04 (HP) en lug wat 360 ppm C02 bevat het. Wortels, anders as wortelknoppies, het 'n betekenisvolle afname in anorganiese P (Pi) ervaar. Onder P-stremming, het lae fosfaat wortels meer pirovaat vanaf malaat gesintetiseer as wortelknoppies, wat 'n definitiewe bydrae vanaf die PEPc roete impliseer. Hiervolgens is pirovaat 'n sleutel metaboliet onder P-stremming. Die belangrikheid van die akkumulering van pirovaat onder P-stremmende toestande is verder beklemtoon deur die toename in metabolisme van PEP via beide die PK- en die PEPcreaksies. Die toename in PK-aktiwiteite is goed gekorreleer met die verhoogde produksie van pirovaat. Onder toestande van P-stremming het die aktiwiteit van PEPc in wortels verhoog, maar nie in wortelknoppies nie. Dit was nie die gevolg van 'n verhoogde uitdrukking van die ensiem nie. Wortel- en wortelknoppie- uitdrukking van PEPc is derhalwe nie gereguleer deur die uitdrukking daarvan nie, maar eerder deur post-tranlasie kontrole. Hierdie resultate vir wortels met wortelknoppies demonstreer dat metaboliese Pi 'n beter maatstaf is om P-stres aan te dui. Hierdie resultate toon dat wortelknoppies beter daartoe instaat is om hul Pi-vlakke en adenilaatvlakke te reguleer, en dit mag ten koste van die gasheerwortel wees. Ons stel voor dat wortelknoppies nie P-stremming tot dieselfde mate ervaar as die gasheerwortel nie en dat dié knoppies optimaal funksioneer by lae Pi vlakke. Die verhoogde konsentrasie van pirovaat, wat vanaf malaat gesintetiseer is, impliseer dat daar 'n groter vereiste is vir dié metaboliet onder toestande van Pstremming. Hierdie studie het die rol van koolstofmetabolismein stikstofbindende organismes, spesifiek onder toestande van fosfaat-tekort, beklemtoon.
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Tapadia, Mrunmai. "Anti-diabetic action of Lupinus angustifolius seed proteins and peptides". Thesis, Curtin University, 2019. http://hdl.handle.net/20.500.11937/77246.
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Lupin seed proteins have been reported to exhibit hypoglycaemic action in animals and humans. This research focuses on exploring in-vitro anti-diabetic action of proteins extracted from Lupinus angustifolius seed. A novel insulin secretory mechanism of extract hydrolysate mediating through Gaq protein signalling pathway in pancreatic ß-cells was established. γ-conglutin protein, a glucose modulating agent, was separated from the extract. Purified γ-conglutin was characterised and hydrolysed to evaluate its anti-diabetic properties in pancreatic ß-cells and myotubes.
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Katroschan, Kai-Uwe [Verfasser]. "Narrow-leaved lupine (Lupinus angustifolius L.) as nitrogen source in organic vegetable production systems / Kai-Uwe Katroschan". Hannover : Technische Informationsbibliothek und Universitätsbibliothek Hannover (TIB), 2011. http://d-nb.info/1013289676/34.
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Boersma, Jeffrey George. "Contributions to the molecular genetics of the Narrow-leaf Lupin (Lupinus augustifolius L.) : mapping, marker development and QTL analysis". University of Western Australia. School of Earth and Geographical Sciences, 2007. http://theses.library.uwa.edu.au/adt-WU2008.0001.
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[Truncated abstract] Narrow-leaf lupin (Lupinus angustifolius L.) was first recorded as having been introduced into Germany during the mid-19th century for use as green manuring and as fodder crops. However, it was not until post World-War I that there was any serious attempt to domesticate the species. Since that time several key domestication genes have been incorporated to enable the species to be grown as a crop over a range of climates, harvested as a bulk commodity and, the seed used for both animal and human consumption. However, the recent domestication of this species has seen a rather limited use of wild germplasm largely as a result of the difficulty in retaining these key domestication genes. To make the task of retaining these genes manageable, it was decided to resort to molecular technology. A mapping population of F8 derived recombinant inbred lines (RILs) has previously been established by the Department of Agriculture and Food, Western Australia, from a cross between a domesticated breeding line 83A:476 and a wild type P27255 in narrow-leaf lupin. The parents together with 89 RILs (of a population of 115) were subjected to DNA fingerprinting using microsatelliteanchored fragment length polymorphism (MFLP) to rapidly generate DNA markers for construction of a linkage map. Five hundred and twenty two unique markers of which 21% were co-dominant, were generated and mapped. Phenotypic data for the domestication traits: mollis (soft seeds), leucospermus (white flower and seed colour); Lentus (reduced pod-shattering), iucundis (low alkaloid), Ku (early flowering) and moustache pattern on seed coats; were included. Three to 7 molecular markers were identified within 5 cM of each of these domestication genes. The anthracnose resistance gene Lanr1 was also mapped. Linkage groups were constructed using MapManager version QTXb20, resulting in 21 linkage groups consisting of 8 or more markers. ... Five pairs of QTLs were found to be involved in epistasis, 2 of these having an effect on early vigour and another 3 influencing the time to opening of the first florets. Variation explained for each trait ranged from 28% for seed size, to 88% for days to flowering. We showed that it was possible to use this data to predict genotypes of superior progeny for these traits under Mediterranean conditions. QTL regions were compared on a second published linkage map and regions of conserved synteny with the model legume Medicago truncatula high-lighted. The work presented in this thesis demonstrates the importance of tight linkage between markers and genes of interest. It is especially important when dealing with genetically diverse material as found in the wild. One of the main problems faced by molecular scientists is the phenomenon known as linkage disequilibrium in marker populations caused by either small population size or 4 insufficient opportunity for recombination. This frequently results in the development of markers with little or no application outside of the population in which it was developed. Although the relatively small size of the population used in this study exposes it to such constraints, in this case excellent and valuable results were achieved in developing useful markers to at least 3 of the domestication traits within a relatively short time period of less then 4 years.
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Stephany, Michael [Verfasser]. "Influence of endogenous enzyme activities on odour-active compound formation in sweet lupin (Lupinus angustifolius L.) / Michael Stephany". Aachen : Shaker, 2016. http://d-nb.info/1104047055/34.
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Baharudin, Sherifah. "The Effects of Heat Treatment and Processing Techniques on the Quality of Australian Sweet Lupin (Lupinus Angustifolius) Flour". Thesis, Curtin University, 2016. http://hdl.handle.net/20.500.11937/59105.
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Lupin has health benefits but low consumer acceptability due the beany flavour caused by Lipoxygenase (LOX) enzyme. To improve this acceptability the beany flavour needs to be removed. Three types of heat treatment were used to reduce the LOX in lupin. Oven heating at 5 minutes and 80˚C was optimum. Up to 40% lupin was incorporated into chapatti without affecting overall acceptability. The results demonstrate that LOX activity can be reduced by heat treatment.
Książki na temat "Lupinus Angustifolius"
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Tarasewicz, Zofia. Biologiczna ocena oligosacharydów wyizolowanych z nasion łubinu wąskolistnego (Lupinus Angustifolius) zastosowanych w żywieniu przepiórek reprodukcyjnych. Szczecin: Akademia Rolnicza w Szczecinie, 1998.
Znajdź pełny tekst źródłaCzęści książek na temat "Lupinus Angustifolius"
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Lim, T. K. "Lupinus angustifolius". W Edible Medicinal And Non-Medicinal Plants, 770–78. Dordrecht: Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-94-007-1764-0_88.
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Azimova, Shakhnoza S., i Anna I. Glushenkova. "Lupinus angustifolius". W Lipids, Lipophilic Components and Essential Oils from Plant Sources, 585. London: Springer London, 2012. http://dx.doi.org/10.1007/978-0-85729-323-7_1910.
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Egle, K., M. F. Soliman, W. Römer i J. Gerke. "Effect of citrate on the uptake of copper and cadmium by Lupinus albus, Lupinus luteus and Lupinus angustifolius". W Plant Nutrition, 468–69. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/0-306-47624-x_226.
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Singh, Karam B., Rhonda C. Foley, Gagan Garg i Lars G. Kamphuis. "Overview of Genomic Resources Available for Lupins with a Focus on Narrow-Leafed Lupin (Lupinus angustifolius)". W Compendium of Plant Genomes, 31–43. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-21270-4_3.
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Brennan, Ross F., i Mike D. A. Bolland. "Lupinus luteus cv. Wodjil takes up more phosphorus and cadmium than Lupinus angustifolius cv. Kalya". W Structure and Functioning of Cluster Roots and Plant Responses to Phosphate Deficiency, 167–85. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-010-0243-1_14.
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Egle, Komi, i Wilhelm Römer. "Cu-, Zn- und Cd-Aufnahme von Lupinus albus L., Lupinus angustifolius L. und Lupinus luteus L. im Vergleich zu Lolium multiflorum Lam". W Physiologie und Funktion von Pflanzenwurzeln, 109–15. Wiesbaden: Vieweg+Teubner Verlag, 2001. http://dx.doi.org/10.1007/978-3-322-87180-0_15.
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Bell, R. W., i K. Frost. "Low Boron Supply Depresses Seed Viability in Canola (Brassica napus L.) and Lupin (Lupinus angustifolius)". W Boron in Plant and Animal Nutrition, 187–95. Boston, MA: Springer US, 2002. http://dx.doi.org/10.1007/978-1-4615-0607-2_17.
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Comino, M. L., M. R. de Felipe, M. Fernandez-Pascual i L. Martin. "Effect of Drought Stress on Carbohydrate Metabolism in Nodules of Lupinus angustifolius". W Eukaryotism and Symbiosis, 449–56. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-60885-8_38.
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Tang, C., B. T. Cobley, S. Mokhtara, C. E. Wilson i H. Greenway. "High pH in the nutrient solution impairs water uptake in lupinus angustifolius L." W Plant Nutrition — from Genetic Engineering to Field Practice, 763–65. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-1880-4_169.
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Ouasti, Imane, Noureddine Chaachouay, Rainer W. Bussmann i Mostafa Elachouri. "Lupinus albus subsp. albus L.Lupinus angustifolius L.Lupinus digitatus Forssk.Lupinus luteus L.Lupinus pilosus L.Fabaceae". W Ethnobotany of Mountain Regions, 1–10. Cham: Springer International Publishing, 2024. http://dx.doi.org/10.1007/978-3-031-13933-8_245-1.
Pełny tekst źródłaStreszczenia konferencji na temat "Lupinus Angustifolius"
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Ptashnik, O. P. "Results of the introduction of Lupinus varieties and samples in the Steppe Crimea". W CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-2020-5-9-10-75.
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Within a framework of Lupinus varieties and samples assessment, we have found that the growing season of Lupinus albus L. was 93, Lupinus angustifolius L. – 99, and Lupinus luteus L. – 95 days under conditions of the Steppe Crimea. The average yield of white lupin seeds was 1.63 t/ha; blue or narrow-leafed lupin – 1.18 t/ha; yellow lupin – 0.72 t/ha. L. albus is more productive compared to L. angustifolius and L. luteus. The seed yield of all studied varieties and samples of white lupin was higher than that of the standard one ‘Michurinsky’. Samples CH-2-17 and CH-78-16 were the most high-yielding (1.77 and 1.74 t/ha, respectively). Variety ‘Belorozovy 144’ was the most promising among the representatives of narrow-leafed lupin; its yield reached 1.64 t/ha. The best in grain size was white lupin; 1000-grains weight was within the range of 200-222g. Varieties of narrowleafed lupin ‘Belorozovy 144’ and ‘Bryansky kormovoy’ contained the least amount of alkaloids (0.021 and 0.022%, respectively).
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Przybylowicz, W. J., J. Mesjasz-Przybylowicz, K. Wouters, K. Vlassak i N. J. J. Combrink. "Micro-PIXE studies of Lupinus angustifolius L. after treatment of seeds with molybdenum". W The fourteenth international conference on the application of accelerators in research and industry. AIP, 1997. http://dx.doi.org/10.1063/1.52517.
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Ageeva, Polina, Natalya Pochutina i Nadezhda MISNIKOVA. "Evaluation of narrow-leafed lupin breeding material for drought resistance and fine seed coat in Bryansk region". W Multifunctional adaptive feed production 27 (75). ru: Federal Williams Research Center of Forage Production and Agroecology, 2022. http://dx.doi.org/10.33814/mak-2022-27-75-44-51.
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The cultivation of grain legumes is an important reserve for increase of high protein feeds production. The narrow-leafed lupin (Lupinus angustifolius L.) is one of the most cultivated species in agriculture. The modern forage varieties are early ripening, tolerate to anthracnose and have high seed protein content and low alkaloid content. The trends in breeding of universal and forage narrow-leafed lupin varieties are drought resistance increase and reduction of seed coat proportion. In early stages of growth and development the estimation of seed ability to grow in sucrose solution under high osmotic pressure is one of the oblique methods for drought resistance determination. These solutions simulate the conditions of physiological soil dryness. Under laboratory conditions 23 narrow-leafed lupin varieties and breeding lines developed in the All-Russian Lupin Research Institute were tested. The variety Vityaz was used as reference; it is listed in the State List of the Russian Federation. The var. Belorozovy 144 and some breeding lines made the first group for high drought resistance level (81–94%). The BL 39-20 has high grain and green mass yield. Eleven varieties and breeding lines included the reference made the second group with the moderate drought resistance level. Lupin has the thick seed coat compared to other legumes; it increases the fiber content and results in digestibility reduction. If the proportion of coat weight and seed weight will be lower, it is possible to hope for the highest nutritive value of grain forage. Among tested varieties and breeding lines the zoned var. Belorozovy 144 has the lowest seed coat content (18.9%). The seed coat content of the breeding lines Uzkolistny 37-12, CmW 62-17 etc. is about 20.5-20.8% among new breeding lines. The breeding line CmW 62-17 stood put for large seeds, this index made 157 g. It overcame the reference by 27 g or by 20.7%.
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Vorob'eva, L., Valeriy Anischenko i Vasily Adamko. "Studying the efficiency of mineral fertilizers and biological products on the productivity and quality of green mass of lupin lupine angustifolia". W Multifunctional adaptive fodder production. ru: Federal Williams Research Center of Forage Production and Agroecology, 2022. http://dx.doi.org/10.33814/mak-2022-28-76-116-122.
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The paper presents the results of studies on the study of different doses of potash fertilizers in combination with phosphate fertilizers, both with a separate application and in combination with the biological product Humimax, when cultivating narrow-leaved lupine for green mass, in a field experiment on soddy-podzolic sandy soil (with a pollution density of 20 and more than Ku/km2). The effectiveness of the influence of doses of potash fertilizers and the biopreparation Humimax on the yield, accumulation of 137Cs and the nutritional value of the green mass of the narrow-leaved lupine was studied. It has been established that the use of the Humimax biopreparation provides an increase in the yield of the green mass of lupin up to 69%. The use of the biological product on all backgrounds of fertilization provides an increase in the content of crude protein in the green mass of lupine. Consistently increasing doses of potassium in combination with P45, both when used alone and in combination with a biological product, reduced the specific activity of 137Cs by more than 2.5 times. To reduce the accumulation of 137Cs in the green mass of lupine below the permissible level, the doses of mineral fertilizers should not be lower thanP45K150.
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Lammi, Carmen. "From the bench to the bedside: the history of lupin bioactive peptides as useful ingredient for the prevention of metabolic syndrome". W 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/bwgm4089.
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Proteins derived from lupin seeds are gaining attention as a source of bioactive peptides. In fact, several pieces of evidence highlight the biological activities of lupin protein hydrolysates and peptides, including hypocholesterolemic, hypoglycemic, antimicrobial, anti-inflammatory, and immunomodulatory effects. The health-promoting activities of lupin peptides have been tested in different animal models and clinical trials. Briefly, diets containing protein from L. albus or L. angustifolius significantly reduce both total cholesterol and low-density lipoprotein (LDL) cholesterol (LDL-C) levels versus control diets containing casein in a rat or hamster models of hypercholesterolemia. An uncontrolled clinical trial on L. albus and two controlled ones on L. angustifolius have confirmed the hypocholesterolemic activity in humans. In light with these evidences and with a more updated perception of the phenomenon, our study suggests that the observed health-promoting activity does not lie in the native protein, but the mixture of peptides generated from the physiological hydrolysis of proteins during digestion. Indeed, many efforts have been pursued in order to characterize the molecular mechanism through which lupin hydrolysates, obtained different hydrolytic enzymes, exert hypocholesterolemic and anti-diabetic effects in vitro and in vivo. In this context, specific peptides responsible of the biological activity exerted by the peptide mixture were also identified and fully characterized from chemical and biological point of view. Doubtless, our results highlight that lupin hydrolysates may be exploited as new ingredients for the development of dietary supplements or functional foods