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Gotowa bibliografia na temat „Lsm Domain”

Autor: Grafiati

Data publikacji: 6 września 2023

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Artykuły w czasopismach na temat "Lsm Domain"

Tritschler, Felix, Ana Eulalio, Vincent Truffault, Marcus D. Hartmann, Sigrun Helms, Steffen Schmidt, Murray Coles, Elisa Izaurralde i Oliver Weichenrieder. "A Divergent Sm Fold in EDC3 Proteins Mediates DCP1 Binding and P-Body Targeting". Molecular and Cellular Biology 27, nr 24 (8.10.2007): 8600–8611. http://dx.doi.org/10.1128/mcb.01506-07.

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ABSTRACT Members of the (L)Sm (Sm and Sm-like) protein family are found across all kingdoms of life and play crucial roles in RNA metabolism. The P-body component EDC3 (enhancer of decapping 3) is a divergent member of this family that functions in mRNA decapping. EDC3 is composed of a N-terminal LSm domain, a central FDF domain, and a C-terminal YjeF-N domain. We show that this modular architecture enables EDC3 to interact with multiple components of the decapping machinery, including DCP1, DCP2, and Me31B. The LSm domain mediates DCP1 binding and P-body localization. We determined the three-dimensional structures of the LSm domains of Drosophila melanogaster and human EDC3 and show that the domain adopts a divergent Sm fold that lacks the characteristic N-terminal α-helix and has a disrupted β4-strand. This domain remains monomeric in solution and lacks several features that canonical (L)Sm domains require for binding RNA. The structures also revealed a conserved patch of surface residues that are required for the interaction with DCP1 but not for P-body localization. The conservation of surface and of critical structural residues indicates that LSm domains in EDC3 proteins adopt a similar fold that has separable novel functions that are absent in canonical (L)Sm proteins.

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Payá, Gloria, Vanesa Bautista, Mónica Camacho, Julia Esclapez i María-José Bonete. "Comprehensive Bioinformatics Analysis of the Biodiversity of Lsm Proteins in the Archaea Domain". Microorganisms 11, nr 5 (3.05.2023): 1196. http://dx.doi.org/10.3390/microorganisms11051196.

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The Sm protein superfamily includes Sm, like-Sm (Lsm), and Hfq proteins. Sm and Lsm proteins are found in the Eukarya and Archaea domains, respectively, while Hfq proteins exist in the Bacteria domain. Even though Sm and Hfq proteins have been extensively studied, archaeal Lsm proteins still require further exploration. In this work, different bioinformatics tools are used to understand the diversity and distribution of 168 Lsm proteins in 109 archaeal species to increase the global understanding of these proteins. All 109 archaeal species analyzed encode one to three Lsm proteins in their genome. Lsm proteins can be classified into two groups based on molecular weight. Regarding the gene environment of lsm genes, many of these genes are located adjacent to transcriptional regulators of the Lrp/AsnC and MarR families, RNA-binding proteins, and ribosomal protein L37e. Notably, only proteins from species of the class Halobacteria conserved the internal and external residues of the RNA-binding site identified in Pyrococcus abyssi, despite belonging to different taxonomic orders. In most species, the Lsm genes show associations with 11 genes: rpl7ae, rpl37e, fusA, flpA, purF, rrp4, rrp41, hel308, rpoD, rpoH, and rpoN. We propose that most archaeal Lsm proteins are related to the RNA metabolism, and the larger Lsm proteins could perform different functions and/or act through other mechanisms of action.

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Guo, Song, i Huazhong Wang. "Image domain least-squares migration with a Hessian matrix estimated by non-stationary matching filters". Journal of Geophysics and Engineering 17, nr 1 (22.11.2019): 148–59. http://dx.doi.org/10.1093/jge/gxz098.

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Abstract Assuming that an accurate background velocity is obtained, least-squares migration (LSM) can be used to estimate underground reflectivity. LSM can be implemented in either the data domain or image domain. The data domain LSM (DDLSM) is not very practical because of its huge computational cost and slow convergence rate. The image domain LSM (IDLSM) might be a flexible alternative if estimating the Hessian matrix using a cheap and accurate approach. It has practical potential to analyse convenient Hessian approximation methods because the Hessian matrix is too huge to compute and save. In this paper, the Hessian matrix is approximated with non-stationary matching filters. The filters are calculated to match the conventional migration image to the demigration/remigration image. The two images are linked by the Hessian matrix. An image deblurring problem is solved with the estimated filters for the IDLSM result. The combined sparse and total variation regularisations are used to produce accurate and reasonable inversion results. The numerical experiments based on part of Sigsbee model, Marmousi model and a 2D field data set illustrate that the non-stationary matching filters can give a good approximation for the Hessian matrix, and the results of the image deblurring problem with combined regularisations can provide high-resolution and true-amplitude reflectivity estimations.

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Böhringer, H. J., D. Boller, J. Leppert, U. Knopp, E. Lankenau, E. Reusche, G. Hüttmann i A. Giese. "Time-domain and spectral-domain optical coherence tomography in the analysis of brain tumor tissue". Lasers in Surgery and Medicine 38, nr 6 (30.05.2006): 588–97. http://dx.doi.org/10.1002/lsm.20353.

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Tritschler, Felix, Ana Eulalio, Sigrun Helms, Steffen Schmidt, Murray Coles, Oliver Weichenrieder, Elisa Izaurralde i Vincent Truffault. "Similar Modes of Interaction Enable Trailer Hitch and EDC3 To Associate with DCP1 and Me31B in Distinct Protein Complexes". Molecular and Cellular Biology 28, nr 21 (2.09.2008): 6695–708. http://dx.doi.org/10.1128/mcb.00759-08.

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ABSTRACT Trailer Hitch (Tral or LSm15) and enhancer of decapping-3 (EDC3 or LSm16) are conserved eukaryotic members of the (L)Sm (Sm and Like-Sm) protein family. They have a similar domain organization, characterized by an N-terminal LSm domain and a central FDF motif; however, in Tral, the FDF motif is flanked by regions rich in charged residues, whereas in EDC3 the FDF motif is followed by a YjeF_N domain. We show that in Drosophila cells, Tral and EDC3 specifically interact with the decapping activator DCP1 and the DEAD-box helicase Me31B. Nevertheless, only Tral associates with the translational repressor CUP, whereas EDC3 associates with the decapping enzyme DCP2. Like EDC3, Tral interacts with DCP1 and localizes to mRNA processing bodies (P bodies) via the LSm domain. This domain remains monomeric in solution and adopts a divergent Sm fold that lacks the characteristic N-terminal α-helix, as determined by nuclear magnetic resonance analyses. Mutational analysis revealed that the structural integrity of the LSm domain is required for Tral both to interact with DCP1 and CUP and to localize to P-bodies. Furthermore, both Tral and EDC3 interact with the C-terminal RecA-like domain of Me31B through their FDF motifs. Together with previous studies, our results show that Tral and EDC3 are structurally related and use a similar mode to associate with common partners in distinct protein complexes.

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Osorio, Luana Nobre, Bruno Pereira-Dias, André Bulcão i Luiz Landau. "Migration deconvolution using domain decomposition". GEOPHYSICS 86, nr 3 (21.04.2021): S247—S256. http://dx.doi.org/10.1190/geo2020-0352.1.

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Least-squares migration (LSM) is an effective technique for mitigating blurring effects and migration artifacts generated by limited data frequency bandwidth, incomplete coverage of geometry, source signature, and unbalanced amplitudes caused by complex wavefield propagation in the subsurface. Migration deconvolution (MD) is an image-domain approach for LSM that approximates the Hessian operator using a set of precomputed point spread functions. We have developed a new workflow by integrating the MD and domain decomposition (DD) methods. DD techniques aim to solve large and complex linear systems by splitting problems into smaller parts, facilitating parallel computing, and providing a higher convergence in iterative algorithms. We suggest that instead of solving the problem in a unique domain, as conventionally performed, splitting the problem into subdomains that overlap and solve each of them independently. We accelerate the convergence rate of the conjugate-gradient solver by applying the DD methods to retrieve better reflectivity, which is mainly visible in regions with low amplitudes. Moreover, using the pseudo-Hessian operator, the convergence of the algorithm is accelerated, suggesting that the inverse problem becomes better conditioned. Experiments using the synthetic Pluto model demonstrate that our algorithm dramatically reduces the required number of iterations while providing a considerable enhancement in image resolution and better continuity of poorly illuminated events.

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Fromont-Racine, Micheline, Andrew E. Mayes, Adeline Brunet-Simon, Jean-Christophe Rain, Alan Colley, Ian Dix, Laurence Decourty i in. "Genome-Wide Protein Interaction Screens Reveal Functional Networks Involving Sm-Like Proteins". Yeast 1, nr 2 (1.01.2000): 95–110. http://dx.doi.org/10.1155/2000/919260.

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A set of seven structurally related Sm proteins forms the core of the snRNP particles containing the spliceosomal U1, U2, U4 and U5 snRNAs. A search of the genomic sequence of Saccharomyces cerevisiae has identified a number of open reading frames that potentially encode structurally similar proteins termed Lsm (L¯ike Sm¯) proteins. With the aim of analysing all possible interactions between the Lsm proteins and any protein encoded in the yeast genome, we performed exhaustive and iterative genomic two-hybrid screens, starting with the Lsm proteins as baits. Indeed, extensive interactions amongst eight Lsm proteins were found that suggest the existence of a Lsm complex or complexes. These Lsm interactions apparently involve the conserved Sm domain that also mediates interactions between the Sm proteins. The screens also reveal functionally significant interactions with splicing factors, in particular with Prp4 and Prp24, compatible with genetic studies and with the reported association of Lsm proteins with spliceosomal U6 and U4/U6 particles. In addition, interactions with proteins involved in mRNA turnover, such as Mrt1, Dcp1, Dcp2 and Xrn1, point to roles for Lsm complexes in distinct RNA metabolic processes, that are confirmed in independent functional studies. These results provide compelling evidence that two-hybrid screens yield functionally meaningful information about protein–protein interactions and can suggest functions for uncharacterized proteins, especially when they are performed on a genome-wide scale.

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Fromont-Racine, Micheline, Andrew E. Mayes, Adeline Brunet-Simon, Jean-Christophe Rain, Alan Colley, Ian Dix, Laurence Decourty i in. "Genome-Wide Protein Interaction Screens Reveal Functional Networks Involving Sm-Like Proteins". Yeast 1, nr 2 (2000): 95–110. http://dx.doi.org/10.1002/1097-0061(20000630)17:2<95::aid-yea16>3.0.co;2-h.

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A set of seven structurally related Sm proteins forms the core of the snRNP particles containing the spliceosomal U1, U2, U4 and U5 snRNAs. A search of the genomic sequence ofSaccharomyces cerevisiaehas identified a number of open reading frames that potentially encode structurally similar proteins termed Lsm (L¯ike Sm¯) proteins. With the aim of analysing all possible interactions between the Lsm proteins and any protein encoded in the yeast genome, we performed exhaustive and iterative genomic two-hybrid screens, starting with the Lsm proteins as baits. Indeed, extensive interactions amongst eight Lsm proteins were found that suggest the existence of a Lsm complex or complexes. These Lsm interactions apparently involve the conserved Sm domain that also mediates interactions between the Sm proteins. The screens also reveal functionally significant interactions with splicing factors, in particular with Prp4 and Prp24, compatible with genetic studies and with the reported association of Lsm proteins with spliceosomal U6 and U4/U6 particles. In addition, interactions with proteins involved in mRNA turnover, such as Mrt1, Dcp1, Dcp2 and Xrn1, point to roles for Lsm complexes in distinct RNA metabolic processes, that are confirmed in independent functional studies. These results provide compelling evidence that two-hybrid screens yield functionally meaningful information about protein–protein interactions and can suggest functions for uncharacterized proteins, especially when they are performed on a genome-wide scale.

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Bhatta, Krishna M., i Norman S. Nishioka. "Effect of pulse duration on microsecond-domain laser lithotripsy". Lasers in Surgery and Medicine 9, nr 5 (1989): 454–57. http://dx.doi.org/10.1002/lsm.1900090505.

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Li, Bo, Minao Sun, Chen Xiang i Yingzhe Bai. "Least-Squares Reverse Time Migration in Imaging Domain Based on Global Space-Varying Deconvolution". Applied Sciences 12, nr 5 (24.02.2022): 2361. http://dx.doi.org/10.3390/app12052361.

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The classical least-squares migration (LSM) translates seismic imaging into a data-fitting optimization problem to obtain high-resolution images. However, the classical LSM is highly dependent on the precision of seismic wavelet and velocity models, and thus it suffers from an unstable convergence and excessive computational costs. In this paper, we propose a new LSM method in the imaging domain. It selects a spatial-varying point spread function to approximate the accurate Hessian operator and uses a high-dimensional spatial deconvolution algorithm to replace the common-used iterative inversion. To keep a balance between the inversion precision and the computational efficiency, this method is implemented based on the strategy of regional division, and the point spread function is computed using only one-time demigration/migration and inverted individually in each region. Numerical experiments reveal the differences in the spatial variation of point spread functions and highlight the importance to use a space-varying deconvolution algorithm. A 3D field case in Northwest China can demonstrate the effectiveness of this method on improving spatial resolution and providing better characterizations for small-scale fracture and cave units of carbonate reservoirs.

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Rozprawy doktorskie na temat "Lsm Domain"

Tuladhar, Kapil. "Lim-only domain proteins in developmental haematopoiesis". Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:d6b73e89-7095-402f-9d9f-4d7837a4db00.

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The production of adult blood initiates from the haematopoietic stem cell (HSC). This clinically important cell has the capacity to maintain all blood lineages throughout the lifetime of an organism. HSCs emerge de novo from the haemogenic endothelium in the ventral wall of the embryonic dorsal aorta, from where they go on to seed adult sites of haematopoiesis. We have shown that Lmo4a is required for the emergence of HSCs in the zebrafish, and go on to demonstrate that Lmo4a regulates expression of the critical transcription factor, gata2a. Strikingly, both over- and under-expression of gata2a in the dorsal aorta severely diminishes HSC production. The LIM-only domain protein Lmo4 has previously been shown to interact with the known haematopoietic regulator, Ldb1. Together with our collaborators, we have identified novel binding partners of Lmo4 in mouse erythroleukaemic cells. Our functional analysis shows that many of these partners are also necessary for HSC emergence, thus revealing several new potential regulators of HSC formation. Given that these proteins were identified in an in vitro model of definitive erythropoiesis, it is remarkable that they also appear to act together in vivo at the level of HSC formation, and our data suggests that a transcriptional complex containing Lmo4 and these partners may directly repress gata2a. The related protein Lmo2 is also known to bind Ldb1. Together with Scl, Lmo2 is a master regulator of the haemangioblast programme. We have been utilising this activity, together with recent structural studies, to identify functionally important residues in the Lmo2 molecule. As a cell’s transcriptional programme drives both normal and pathological development, and misexpression of both Lmo2 and Lmo4 is involved in a variety of oncogenic states, the work presented in this thesis is likely to inform efforts to develop therapeutically relevant reagents.

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Pinto, Belinda Sophia Geyer Pamela Kent. "Understanding the role of LEM domain proteins in Drosophila development". [Iowa City, Iowa] : University of Iowa, 2009. http://ir.uiowa.edu/etd/421.

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Jurata, Linda Wagner. "Identification and analysis of the nuclear LIM domain interactor NLI /". Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 1998. http://wwwlib.umi.com/cr/ucsd/fullcit?p9904815.

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Pinto, Belinda Sophia. "Understanding the role of LEM domain proteins in Drosophila development". Diss., University of Iowa, 2009. https://ir.uiowa.edu/etd/421.

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The nuclear lamina is a filamentous network that underlies the nuclear envelope. Lamina components include the family of LEM domain (LEM-D) proteins, named for LAP2, emerin and MAN1. Mutations in genes encoding LEM-D proteins cause tissue-restricted human disease, even though these genes are globally expressed. To understand the contributions of the LEM-D proteins to nuclear lamina function, investigations of the Drosophila LEM-D proteins was undertaken. The Drosophila genome encodes four LEM-D proteins and this thesis describes work done on the Drosophila homologues of MAN1 and emerin, Drosophila MAN1 (dMAN1) and Otefin (Ote). Chapter 2 describes the generation and phenotypic analyses of dMAN1 mutants. These mutants display a range of tissue-specific defects associated with an increase in BMP/Dpp signaling. This suggests that dMAN1 downregulates BMP/Dpp signaling at the nuclear periphery. Chapter 3 describes the identification and phenotypic analyses of ote mutants. Loss of Ote is associated with a tissue-specific defect of the female germline where ote mutant females display defects in germline stem cell (GSC) maintenance. Loss of Ote causes defects in the germline cells, the cap cells of GSC niche and an increased sensitivity to Dpp signaling in both germline and somatic cells. These findings support models suggesting that laminopathies arise from dysfunction of the homeostasis in stem cell populations. Taken together, these studies suggest that the nuclear lamina may play tissue-specific roles through regulation of signal transduction pathways. Our data also support the use of Drosophila as a system to elucidate the mechanistic basis of diseases associated with defects in the nuclear lamina.

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Diefenbacher, Markus Elmar. "The transcriptional co-activator function of the LIM-domain protein nTrip6". Eggenstein-Leopoldshafen Forschungszentrum Karlsruhe GmbH, 2010. http://d-nb.info/1002907535/34.

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Nisevic, Ivan. "Detection and analysis of LIM domain-mediated interactions between transcription factors". Thesis, The University of Sydney, 2015. http://hdl.handle.net/2123/15711.

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LIM-homeodomain (LIM-HD) proteins are a class of transcription factors involved in tissue specification and cell determination during development and are important in adult gene regulation. Six families of LIM-HD proteins, with two close paralogues in each family, are commonly found in tetrapods. They bind DNA via HDs, whereas their interactions with other proteins are mediated mainly by a pair of closely spaced LIM-domains (LIMs) in each protein. These proteins take part in various transcriptional complexes with Ldb1 and other cofactors that contain LIM-interaction domains (LIDs). In this thesis, protein-protein interactions of LIM-HD proteins were analysed in order to better understand the molecular mechanisms of transcriptional complex formation. Based on previous research that showed LIM-LID mediated interactions between Lhx3 and Isl1, yeast two-hybrid mating arrays were used to investigate how widespread protein-protein interactions are amongst the 12 mammalian LIM-HD proteins. Due to high levels of background growth in experiments with full-length proteins in pGBT9 vectors, the mating arrays focused on LIM-domain mediated interactions with full-length LIM-HDs or known LIDs. The arrays revealed a relatively strong interaction between Lhx3 (or Lhx4) and Isl1 (or Isl2), and detected weaker interactions between Lmx1a or Lmx1b and the LIM-binding domain of Isl1. The contribution of separate LIM-domains to the overall interaction with Ldb1 for each of the proteins was analysed by the same method. In most cases one of the LIM domains in each protein was able to independently interact with the LID domain of Ldb1 by yeast two-hybrid analysis indicating a dominant binder: LIM1 in Isl1 and Isl2, or LIM2 in other proteins. The exceptions were paralogues Lhx1 and Lhx5, for which no separate domain showed interaction with Ldb1LID by this approach. All tandem LIM-domain constructs showed a much stronger interaction with Ldb1LID than any isolated LIM domain supporting the idea that both domains are required for high affinity binding to Ldb1. Bimolecular Fluorescence Complementation experiments in yeast were designed and conducted as an alternative approach to test interactions between full-length LIM-HD proteins in the hope that a non-transcription based assay would lead to no or less background signal compared to yeast two-hybrid analysis. A plasmid system was developed based on existing yeast two-hybrid vectors using split green fluorescent proteins in place of domains from the GAL4 transcription factor. The assay was able to detect interactions between different LIMs and their partners but unfortunately interactions between full-length proteins were still difficult to detect due to low fluorescence, self-complementation in the controls and localization effects. LIM domains from LIM-HD proteins cannot be used in standard bimolecular binding assays because they tend to be insoluble and/or aggregate in the absence of a binding partner. Stable, soluble intramolecular ‘tethered complexes’ can be generated in which LIMs are tethered to Ldb1LID via a flexible linker. Introduction of a specific protease site into the tether allows the formation of intermolecular cut complexes, which have previously been used in homologous competition ELISA experiments. In this thesis attempts were made to develop more robust biophysical binding assays that could be used to assess the binding affinities of different LIMs for Ldb1LID. Several different labelling approaches were used to generate proteins with fluorescent tags for use in fluorescence anisotropy assays. In one of these approaches expressed protein ligation was applied to generate proteins with an N-terminal fluorescein. Although this labelling strategy was of low efficiency for LIMs-Ldb1LID tethered constructs, some preliminary fluorescence anisotropy experiments were carried out, which indicated that this could be a useful strategy providing a more efficient labelling strategy can be found. GFP-tagged tethered complexes were easier to generate, but could not be used in anisotropy experiments because of the intrinsically high anisotropy of GFP proteins. However, preliminary experiments indicated that these proteins can be used in clear native gel shift competition assays to compare binding affinities of different tandem LIM domains to Ldb1LID. Data presented in this thesis provide valuable insight into protein-protein interactions of LIM-HD transcription factors and the advantages, as well as disadvantages, of applied experimental approaches. The results and their implications are discussed, raising questions that can be resolved in future studies.

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Gu, Wenchao. "Exploring the roles of LIM domain binding proteins in zebrafish development". Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:54f520f6-170a-480a-a195-1a0739055031.

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As some of the most important and widely utilised intercellular signalling molecules, transforming growth factor βs (TGFβs) play critical roles in normal development and in human disease. Establishing appropriate levels of signalling involves positive and negative feedback, driven by the same signal transduction components, but whether or how the two are distinguished has not previously been understood. Here we show that LIM domain binding proteins (Ldbs) drive the Smad6/7-mediated negative feedback of TGFβ signalling, but they are not required for the ligand-driven positive feedback or other downstream transcriptional activation. In Ldb-deficient zebrafish embryos, the homeostasis of TGFβ signalling is perturbed. As a consequence, signalling of TGFβ family members, Nodal and BMP, is stably enhanced, giving rise to excess mesoderm and endoderm, an effect that can be rescued by reducing Nodal and BMP. Later in development, conditional ldb2a knockdown causes defective vascular, angiogenic and haemogenic development, likely also by elevating TGFβ signalling. Thus, Ldbs control the homeostatic regulation of TGFβ signalling and therefore play critical roles in diverse developmental processes.

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Klaavuniemi, T. (Tuula). "PDZ-LIM domain proteins and α-actinin at the muscle Z-disk". Doctoral thesis, University of Oulu, 2006. http://urn.fi/urn:isbn:9514282647.

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Abstract The Z-disk is a sophisticated structure that connects adjacent sarcomeres in striated muscle myofibrils. α-Actinin provides strength to the Z-disks by crosslinking the actin filaments of adjacent sarcomeres. α-Actinin is an antiparallel homodimer, composed of an N-terminal actin binding domain (ABD), the central rod domain, and two pairs of C-terminal EF-hands. The PDZ-LIM domain proteins interact with α-actinin at the Z-disk. Of these proteins, only the actinin-associated LIM protein (ALP), Z-band alternatively spliced PDZ-containing protein (ZASP/Cypher) and C-terminal LIM protein (CLP36) have a ZASP/Cypher-like (ZM) motif consisting of 26-27 conserved residues in the internal region between the PDZ and LIM domains. The aim of this work was to understand the molecular interplay between the ZM-motif containing members of the PDZ-LIM proteins and α-actinin. To unveil the biological relevance of the interaction between the PDZ-LIM proteins and α-actinin, naturally occurring human ZASP/Cypher mutations were analyzed. Two interaction sites were found between ALP, CLP36 and α-actinin using recombinant purified proteins in surface plasmon resonance (SPR) analysis. The PDZ domain of ALP and CLP36 recognized the C-terminus of α-actinin, whereas the internal regions bound to the rod domain. Further characterization showed that the ALP internal region adopts and extended conformation when interacting with α-actinin and that the ZM-motif partly mediated the interaction, but did not define the entire interaction area. ZASP/Cypher also interacted and competed with ALP in binding to the rod domain. The internal fragments containing the ZM-motif were important for co-localization of ALP and ZASP/Cypher with α-actinin at the Z-disks and on stress fibers. The absence of ALP and ZASP/Cypher in focal contacts indicates that other interacting molecules, for instance vinculin and integrin, may compete in binding to the rod in these areas or additional proteins are required in targeting to these locations. The co-localization of the ZASP/Cypher with α-actinin could be released by disrupting the stress fibers leading to an accumulation of α-actinin in the cell periphery, whereas ZASP/Cypher was not in these areas. This suggests that an intact cytoskeleton is important for ZASP/Cypher interaction with α-actinin. Earlier studies have shown that mutations in the ZASP/Cypher internal region are associated with muscular diseases. These mutations, however, did not affect ZASP/Cypher co-localization with α-actinin or the stability of ZASP/Cypher proteins. The Z-disk possesses a stretch sensor, which is involved in triggering hypertrophic growth as a compensatory mechanism to increased workloads. α-Actinin is a docking site of molecules that are involved in hypertrophic signaling cascades mediated by calsarcin-calcineurin and protein kinase C (PKC) isoforms. The internal interaction site may be involved in targeting PKCs, which bind to the LIM domains of ZASP/Cypher, to the Z-disks. The similar location of the internal interaction site with calsarcin on the rod suggests that ZASP/Cypher, ALP and CLP36 may regulate calsarcin-mediated hypertrophic signaling.

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Duan, Jie. "Active Control of Vehicle Powertrain Noise Applying Frequency Domain Filtered-x LMS Algorithm". University of Cincinnati / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1243614246.

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Khurana, Bharat. "Characterization of DLIM1, a novel cytoskeleton-associated LIM domain containing protein of Dictyostelium discoideum". [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=961945737.

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Książki na temat "Lsm Domain"

Bod-rang-skyong-ljongs ʼphel rgyas dang sgyur bcos u yon lhan khang. Gzhung lam gyi bdag dbang ham bzung dang gtor skyon btang bar skyin tshab (gun gsab) gron dngul bsdu phyogs kyi rnam grangs dang tshad gzhiʼi skor gyi brda tho: Bod ʼphel sgyur rin gong [2009] ang 651 pa. [Tibet]: Bod-rang-skyong-ljongs ʼgrim ʼgrul thing gzhung lam do dam cus nas par du skrun, 2009.

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Ouhabaz, El-Maati. Analysis of Heat Equations on Domains. (LMS-31). Princeton University Press, 2009.

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Analysis of Heat Equations on Domains. (Lms-31). Princeton University Press, 2009.

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Chan, Ka Nin. Two-dimensional beamforming using a frequency domain complex Least Mean-Squares (LMS) adaptive filter. 1986.

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Ouhabaz, El-Maati. Analysis of Heat Equations on Domains (LMS-31) (London Mathematical Society Monographs). Princeton University Press, 2004.

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Części książek na temat "Lsm Domain"

Platz, Roland. "Approach to Assess Basic Deterministic Data and Model Form Uncertaint in Passive and Active Vibration Isolation". W Lecture Notes in Mechanical Engineering, 208–23. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-77256-7_17.

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AbstractThis contribution continues ongoing own research on uncertainty quantification in structural vibration isolation in early design stage by various deterministic and non-deterministic approaches. It takes into account one simple structural dynamic system example throughout the investigation: a one mass oscillator subject to passive and active vibration isolation. In this context, passive means that the vibration isolation only depends on preset inertia, damping, and stiffness properties. Active means that additional controlled forces enhance vibration isolation. The simple system allows a holistic, consistent and transparent look into mathematical modeling, numerical simulation, experimental test and uncertainty quantification for verification and validation. The oscillator represents fundamental structural dynamic behavior of machines, trusses, suspension legs etc. under variable mechanical loading. This contribution assesses basic experimental data and mathematical model form uncertainty in predicting the passive and enhanced vibration isolation after model calibration as the basis for further deterministic and non-deterministic uncertainty quantification measures. The prediction covers six different damping cases, three for passive and three for active configuration. A least squares minimization (LSM) enables calibrating multiple model parameters using different outcomes in time and in frequency domain from experimental observations. Its adequacy strongly depends on varied damping properties, especially in passive configuration.

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Uribe, Diego. "LSA Based Approach to Domain Detection". W Lecture Notes in Computer Science, 62–69. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-13647-9_7.

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Jurata, L. W., i G. N. Gill. "Structure and Function of LIM Domains". W Protein Modules in Signal Transduction, 75–113. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-80481-6_4.

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Wild, Fridolin. "Calibrating for Specific Domains". W Learning Analytics in R with SNA, LSA, and MPIA, 149–63. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-28791-1_7.

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Shiva Kumar, K. B., K. B. Raja, R. K. Chhotaray i Sabyasachi Pattnaik. "Integration of Spatial and Transform Domain in LSB Steganography". W Computer Networks and Information Technologies, 400–402. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-19542-6_74.

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Nanda, V. C. "On the gcd and lcm of Matrices Over Dedekind Domains". W Number Theory and Discrete Mathematics, 201–11. Basel: Birkhäuser Basel, 2002. http://dx.doi.org/10.1007/978-3-0348-8223-1_20.

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Nanda, V. C. "On the gcd and lcm of Matrices Over Dedekind Domains". W Number Theory and Discrete Mathematics, 201–11. Gurgaon: Hindustan Book Agency, 2002. http://dx.doi.org/10.1007/978-93-86279-10-1_20.

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Popova, Svetlana, Vera Danilova i Artem Egorov. "Clustering Narrow-Domain Short Texts Using K-Means, Linguistic Patterns and LSI". W Communications in Computer and Information Science, 183–89. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-12580-0_18.

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Motz, Christian, Oliver Ploder, Thomas Paireder i Mario Huemer. "Enhanced Transform-Domain LMS Based Self-interference Cancellation in LTE Carrier Aggregation Transceivers". W Computer Aided Systems Theory – EUROCAST 2019, 28–35. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-45096-0_4.

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Tovar, Mireya, David Pinto, Azucena Montes i Gabriel González. "An Approach Based in LSA for Evaluation of Ontological Relations on Domain Corpora". W Lecture Notes in Computer Science, 225–33. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-59226-8_22.

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Streszczenia konferencji na temat "Lsm Domain"

Bahmani, Bahador, i Amir R. Khoei. "Modeling Convective Heat Propagation in a Fractured Domain With X-FEM and Least Square Method". W ASME 2017 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/imece2017-71167.

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The main goal of the current study is developing an advanced and robust numerical tool for accurate capturing heat front propagation. In some applications such as impermeable medium, Heat transfer in the surrounding domain of fracture acts just as a conduction process but the heat transfer through the fractures appears as a convection process. From a mathematical point of view, a parabolic partial differential equation (PDE) should be solved in the surrounding domain whereas a hyperbolic PDE should be solved in the domain of fractures. In fact, they have completely different treatments and this is one of the complicated problems in this area. In this paper, the presence of fractures and discontinuities are considered with the aim of eXtended Finite Element Method (X-FEM). In the proposed numerical approach, the domain is decomposed into local and global scales. Global and local domains are solved by the X-FEM and Least Square Method (LSM) techniques, respectively. As a final result, it is determined that the treatment of coupling term between two scales is one of the most important factors for system performance. Increasing its effect can significantly improve the efficiency of the whole system.

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Xu, Xiaoqiang, Shikui Chen, Xianfeng David Gu i Michael Yu Wang. "Conformal Topology Optimization of Heat Conduction Problems on Manifolds Using an Extended Level Set Method (X-LSM)". W ASME 2021 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2021. http://dx.doi.org/10.1115/detc2021-67819.

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Abstract In this paper, the authors propose a new dimension reduction method for level-set-based topology optimization of conforming thermal structures on free-form surfaces. Both the Hamilton-Jacobi equation and the Laplace equation, which are the two governing PDEs for boundary evolution and thermal conduction, are transformed from the 3D manifold to the 2D rectangular domain using conformal parameterization. The new method can significantly simplify the computation of topology optimization on a manifold without loss of accuracy. This is achieved due to the fact that the covariant derivatives on the manifold can be represented by the Euclidean gradient operators multiplied by a scalar with the conformal mapping. The original governing equations defined on the 3D manifold can now be properly modified and solved on a 2D domain. The objective function, constraint, and velocity field are also equivalently computed with the FEA on the 2D parameter domain with the properly modified form. In this sense, we are solving a 3D topology optimization problem equivalently on the 2D parameter domain. This reduction in dimension can greatly reduce the computing cost and complexity of the algorithm. The proposed concept is proved through two examples of heat conduction on manifolds.

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Tian, Jiawei, Xuanhe Zhao, Xianfeng David Gu i Shikui Chen. "Designing Conformal Ferromagnetic Soft Actuators Using Extended Level Set Methods (X-LSM)". W ASME 2020 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2020. http://dx.doi.org/10.1115/detc2020-22438.

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Abstract Ferromagnetic soft materials (FSM) can generate flexible movement and shift morphology in response to an external magnetic field. They have been engineered to design products in a variety of promising applications, such as soft robots, compliant actuators, or bionic devices, et al. By using different patterns of magnetization in the soft elastomer matrix, ferromagnetic soft matters can achieve various shape changes. Although many magnetic soft robots have been designed and fabricated, they are limited by the designers’ intuition. Topology optimization (TO) is a systematically mathematical method to create innovative structures by optimizing the material layout within a design domain without relying on the designers’ intuition. It can be utilized to architect ferromagnetic soft active structures. Since many of these ‘soft machines’ exist in the form of thin-shell structures, in this paper, the extended level set method (X-LSM) and conformal mapping theory are employed to carry out topology optimization of the ferromagnetic soft actuator on manifolds. The objective function consists of a sub-objective function for the kinematics requirement and a sub-objective function for minimum compliance. Shape sensitivity analysis is derived using the material time derivative and adjoint variable method. Two examples, including a circular shell actuator and a flytrap structure, are studied to demonstrate the effectiveness of the proposed framework.

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Nakagawa, Tadahiro, Naoki Shikazono i Nobuhide Kasagi. "Numerical Simulation of Electrochemical Reaction in Reconstructed Three-Dimensional LSM/YSZ Composite Cathode". W ASME 2008 6th International Conference on Fuel Cell Science, Engineering and Technology. ASMEDC, 2008. http://dx.doi.org/10.1115/fuelcell2008-65027.

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In the present study, a novel computational scheme for the assessment of the activation polarization of LSM/YSZ composite cathodes is proposed. The scheme consists of modeling techniques of three-dimensional microstructures and an evaluation method of electrochemical characteristics. Two modeling techniques of microstructures are employed, i.e. the stochastic reconstruction (SR) method and the random packing model (RPM). In the SR method, the 3-D structure is reconstructed statistically from the two-point correlation function of the cross-sectional image of SEM-EDX. In RPM, on the other hand, spherical LSM and YSZ particles are randomly packed in the computational domain. This model is mainly used for the parametric survey, because control parameters used in the model have good correspondence to the parameters used in the actual cell manufacturing process. The lattice Boltzmann method coupled with the Butler-Volmer equation is employed for the detailed assessment of the electrochemical characteristics inside the constructed 3-D cathode microstructures. The oxygen diffusion and the electronic and ionic conductions are calculated simultaneously, and coupled with the charge transfer at the three-phase boundary (TPB) using the Butler-Volmer equation. As a result, potential, polarization and current density distributions are fully investigated. The results from the SR method reveal that the cathode sintered at 1150 °C shows the smaller overpotential than the cathodes sintered at 1200 and 1250 °C. The RPM results show that particle diameter and its standard deviation as well as volume fraction of species have large effects on the cathode performance.

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Gindrat, M., A. Refke i R. Damani. "APS-Triplex and LPPS-Thin Film as Advanced Plasma Spraying Technologies for Industrialization of SOFC Components". W ITSC2008, redaktorzy B. R. Marple, M. M. Hyland, Y. C. Lau, C. J. Li, R. S. Lima i G. Montavon. Verlag für Schweißen und verwandte Verfahren DVS-Verlag GmbH, 2008. http://dx.doi.org/10.31399/asm.cp.itsc2008p0088.

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Abstract Reliable and economically efficient processes are necessary for the production of high quality coatings for solid oxide fuel cells (SOFC) applications in an industrial scale. In that perspective, Sulzer Metco developed several coating solutions through different processes adapted for each specific applications, in particular on metal supported cells (MSC). Diffusion barrier layers (DBL) using perovskite material, such as Lanthanum Strontium Manganite (LSM), is produced “state-of-the-art” as coating service by Sulzer Metco on metallic interconnects (IC) using the Triplex technology. The newly developed TriplexPro-200 having a long lifetime performance and specific features, like cascaded arc and 3-cathode torch, is the best candidate for producing high quality and reliable coatings in a mass production of SOFC functional layers. LPPS-Thin Film, on the other hand is the technology of choice to deposit very dense, thin and homogeneous layers on various substrates. Yttria stabilized Zirconia (YSZ) layers of 20-40 µm thickness have been deposited on thin metallic substrates (0.7 mm, 140 cm2) without producing any strong deformation of the substrate. Considering the dimension of the metallic substrate the coated cells present very good gas leak tightness performances between 2 and 8 Pa·m/s which is homogeneous on the substrate area. Moreover, LPPS-TF can also be used to produce very dense and thin LSM coatings on interconnects. In this case, LPPS-TF not only produces denser and thinner coatings but also becomes again competitive when considering the manufacturing of DBL for metallic ICs on a high production scale. This paper presents the current developments of these technologies in the domain of SOFC applications.

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Hosur, S., i A. H. Tewfik. "Wavelet transform domain LMS algorithm". W Proceedings of ICASSP '93. IEEE, 1993. http://dx.doi.org/10.1109/icassp.1993.319546.

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Lott, Dawn A., Adrienne J. Raglin i Somiya Metu. "Decision making with uncertainty using the LRM method: MATLAB versus Java". W Artificial Intelligence and Machine Learning for Multi-Domain Operations Applications III, redaktorzy Tien Pham, Latasha Solomon i Myron E. Hohil. SPIE, 2021. http://dx.doi.org/10.1117/12.2585826.

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Iqbal, Naveed, Murwan Bashir i Azzedine Zerguine. "Convex Combination of Transform Domain LMS and Sparse LMS". W 2018 52nd Asilomar Conference on Signals, Systems, and Computers. IEEE, 2018. http://dx.doi.org/10.1109/acssc.2018.8645476.

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Mason, Robert. "Interoperability Gap Challenges for Learning Object Repositories & Learning Management Systems". W InSITE 2007: Informing Science + IT Education Conference. Informing Science Institute, 2007. http://dx.doi.org/10.28945/3079.

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An interoperability gap exists between Learning Management Systems (LMS) and Learning Ob ject Repositories (LOR). LORs are responsible for the storage and management of Learning Objects and the associated Learning Object Metadata (LOM). LOR(s) adhere to various LOM standards depending up the requirements established by user groups and LOR administrators. Two common LOM standards found in LORs are CanCore (Canadian LOM standard) and the Sharable Content Object Reference Model (SCORM) Content Aggregation Model (CAM). In contrast, LMSs are independent computer systems that manage and deliver course content to students via a web interface. This research addresses three important issues related to this problem domain: (a) a lack of metadata standards that define the format of how assessment data should be communicated from Learning Management Systems to Learning Object Repositories, (b) a lack of Information Engineering (IE) architectural standards for the transfer of data from Learning Management Systems to Learning Object Repositories, and (c) a lack of middleware that facilitates the movement of the assessment data from the Learning Management Systems to Learning Object Repositories. Thus, the three goals of this research are: (a) make recommendations for extending the CanCore and SCORM CAM LOM standards to facilitate the storage of assessment and summary assessment data, (b) define the foundation for an IE architectural standard based on an Access Control Policy (ACP) and Data Validation Policy (DVP) using a reliable consensus of experts with the Delphi technique, and (c) develop a middleware prototype that transfers learning assessment data from multiple Learning Management Systems into the Learning Object Metadata of Learning Objects that are stored within a CanCore or SCORM compliant Learning Object Repository.

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Ogunfunmi, Tokunbo. "Implementation of the Hartley-Transform-Based Block LMS Algorithm". W ASME 1993 International Computers in Engineering Conference and Exposition. American Society of Mechanical Engineers, 1993. http://dx.doi.org/10.1115/cie1993-0091.

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Abstract This paper presents a cost-effective The Frequency-domain Least-Mean-Square (FLMS) adaptive algorithm (or more generally the Transform-domain LMS adaptive algorithm) [12], [13] has mainly two advantages over the conventional LMS algorithm [19]. The first is that it overcomes the slow convergence of the LMS algorithm by orthogonalizing the input (thereby performing better than the LMS for correlated input signals) and the second advantage is that it can be used for implementing the time-domain Block LMS (BLMS) algorithms as well [18]. The Hartley transform is a newly introduced real-to-real transform that is a suitable replacement for the complex Fourier transform [1] and [2] in several adaptive filtering applications such as adaptive interference cancellation that has wide applicability to problems in telecommunications, biomedical engineering, etc. The realization of the Transform-domain BLMS adaptive algorithm based on the Discrete Hartley Transforms (DHT) and its implementation on the TMS320C30 digital signal processor chip is described.

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Raporty organizacyjne na temat "Lsm Domain"

Moskowitz, Ira S., Patricia A. Lafferty i Farid Ahmed. On LSB Spatial Domain Steganography and Channel Capacity. Fort Belvoir, VA: Defense Technical Information Center, marzec 2008. http://dx.doi.org/10.21236/ada489843.

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Ikejiri, Y. Analysis of Inter-Domain Label Switched Path (LSP) Recovery. Redaktorzy T. Takeda i A. Farrel. RFC Editor, sierpień 2008. http://dx.doi.org/10.17487/rfc5298.

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Tan, Peng, i Nicholas Sitar. Parallel Level-Set DEM (LS-DEM) Development and Application to the Study of Deformation and Flow of Granular Media. Pacific Earthquake Engineering Research Center, University of California, Berkeley, CA, marzec 2023. http://dx.doi.org/10.55461/kmiz5819.

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We present a systematic investigation of computational approaches to the modeling of granular materials. Granular materials are ubiquitous in everyday life and in a variety of engineering and industrial applications. Despite the apparent simplicity of the laws governing particle-scale interactions, predicting the continuum mechanical response of granular materials still poses extraordinary challenges. This is largely due to the complex history dependence resulting from continuous rearrangement of the microstructure of granular material, as well as the mechanical interlocking due to grain morphology and surface roughness. X-Ray Computed Tomography (XRCT) is used to characterize the grain morphology and the fabric of the granular media, naturally deposited sand in this study. The Level-Set based Discrete Element Method (LS-DEM) is then used to bridge the granular behavior gap between the micro and macro scale. The LS-DEM establishes a one-to-one correspondence between granular objects and numerical avatars and captures the details of grain morphology and surface roughness. However, the high-fidelity representation significantly increases the demands on computational resources. To this end a parallel version of LS-DEM is introduced to significantly decrease the computational demands. The code employs a binning algorithm, which reduces the search complexity of contact detection from O(n2) to O(n), and a domain decomposition strategy is used to elicit parallel computing in a memory- and communication-efficient manner. The parallel implementation shows good scalability and efficiency. High fidelity LS avatars obtained from XRCT images of naturally deposited sand are then used to replicate the results of triaxial tests using the new, parallel LS-DEM code. The result show that both micro- and macro-mechanical behavior of natural material is well captured and is consistent with experimental data, confirming experimental observation that the primary source of peak strength of sand is the mechanical interlocking between irregularly shaped grains. Specifically, triaxial test simulations with a flexible membrane produce a very good match to experimentally observed relationships between deviatoric stress and mobilized friction angle for naturally deposited sand. We then explore the viability of modeling dynamic problems with a new formulation of an impulse based LS-DEM. The new formulation is stable, fast, and energy conservative. However, it can be numerically stiff when the assembly has substantial mass differences between particles. We also demonstrate the feasibility of modeling deformable structures in the rigid body framework and propose several enhancements to improve the convergence of collision resolution, including a hybrid time integration scheme to separately handle at rest contacts and dynamic collisions. Finally, we extend the impulse-based LS-DEM to include arbitrarily shaped topographic surfaces and exploit its algorithmic advantages to demonstrate the feasibility of modeling realistic behavior of granular flows. The novel formulation significantly improves performance of dynamic simulations by allowing larger time steps, which is advantageous for observing the full development of physical phenomena such as rock avalanches, which we present as an illustrative example.

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Applebaum, Shalom W., Lawrence I. Gilbert i Daniel Segal. Biochemical and Molecular Analysis of Juvenile Hormone Synthesis and its Regulation in the Mediterranean Fruit Fly (Ceratitis capitata). United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7570564.bard.

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Original Objectives and revisions: (1) "To determine the biosynthetic pathway of JHB3 in the adult C. capitata CA in order to establish parameters for the future choice and synthesis of suitable inhibitors". Modified: to determine the pattern of FR-7 biosynthesis during normal reproductive maturation, and identify enzymes potentially involved in its synthesis. (2) "To correlate allatal epoxidase activity to the biosynthesis of JHB3 at different stages of reproductive maturation/vitellogenesis and evaluate the hypothesis that a specific JH-epoxidase may be rate limiting". Modified: to study the effects of epoxidase inhibitors on the pattern of allatal JH biosynthesis in vitro and on female reproduction in vive. (3) "To probe and clone the gene homologous to ap from C. capitata, determine its exon-intron organization, sequence it and demonstrate its spatial and temporal expression in larvae, pupae and adults." The "Medfly" (Ceratitis capitata) is a serious polyphagous fruit pest, widely distributed in subtropical regions. Damage is caused by oviposition and subsequent development of larvae. JH's are dominant gonadotropic factors in insects. In the higher Diptera, to which the Medfly belongs, JHB3 is a major homolog. It comprises 95% of the total JH produced in vitro in D. melanogaster, with JH-III found as a minor component. The biosynthesis of both JH-III and JHB3 is dependent on epoxidation of double bonds in the JH molecule. The specificity of such epoxidases is unknown. The male accessory gland D. melanogaster produces a Sex Peptide, transferred to the female during copulation. SP reduces female receptivity while activating specific JH biosynthesis in vitro and inducing oviposition in vive. It also reduces pheromone production and activates CA of the moth Helicoverpa armigera. In a previous study, mutants of the apterous (ap) gene of D. melanogaster were analyzed. This gene induces previteilogenic arrest which can be rescued by external application of JH. Considerable progress has been made in recombinant DNA technology of the Medfly. When fully operative, it might be possible to effectively transfer D. melanogaster endocrine gene-lesions into the Medfly as a strategy for their genetic control. A marked heterogeneity in the pattern of JH homologs produced by Medfly CA was observed. Contrary to the anticipated biosynthesis of JHB;, significant amounts of an unknown JH-like compound, of unknown structure and provisionally termed FR-7, were produced, in addition to significant amounts of JH-III and JHB3. Inhibitors of monooxygenases, devised for their effects on ecdysteroid biosynthesis, affect Medfly JH biosynthesis but do not reduce egg deposition. FR-7 was isolated from incubation media of Medfly CA and examined by various MS procedures, but its structure is not yet resolved. MS analysis is being done in collaboration with Professor R.R.W. Rickards of the Australian National University in Canberra, Australia. A homologue of the ap gene of D. melanogaster exists in the Medfly. LIM domains and the homeo-domain, important for the function of the D. melanogaster ap gene, are conserved here too. Attempts to clone the complete gene were unsuccessful. Due to the complexity of JH homologs, presence of related FR-7 in the biosynthetic products of Medfly CA and lack of reduction in eggs deposited in the presence of monooxygenase inhibitors, inhibition of epoxidases is not a feasible alternative to control Medfly reproduction, and raises questions which cannot be resolved within the current dogma of hormonal control of reproduction in Diptera. The Medfly ap gene has similar domains to the D. melanogaster ap gene. Although mutant ap genes are involved in JH deficiency, ap is a questionable candidate for an endocrine lesion, especially since the D. melanogoster gene functions is a transcription factor.

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