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Kotland, Vojtěch. "Separace lipidů z buněčných tkání". Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2019. http://www.nusl.cz/ntk/nusl-401857.
Pełny tekst źródłaDennison, Andrew. "Neutron reflectivity studies of insulin and phosphatidylcholine floating lipid bilayers". Thesis, University of Sheffield, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.574586.
Pełny tekst źródłaWood, David. "Lipid Screening and Lipid Disorders in Children". Digital Commons @ East Tennessee State University, 2020. https://dc.etsu.edu/etsu-works/7684.
Pełny tekst źródłaDeeney, Jude T. "Micro lipid droplet precursors of milk lipid globules". Thesis, Virginia Tech, 1985. http://hdl.handle.net/10919/45673.
Pełny tekst źródłaMaster of Science
Bandegi, Sanaz. "INTERACTION OF FLUORESCENT LIPID DYES WITH LIPID VESICLES AND SUPPORTED LIPID BILAYERS AND THEIR APPLICATIONS". Diss., Temple University Libraries, 2019. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/584744.
Pełny tekst źródłaPh.D.
Lipophilic dye probes are widely used for labelling of cells, organelles, liposomes, viruses and lipoproteins. The lipophilic dye diffuses in the membrane and stains the cell and cells even tolerate the lipophilic dye in high concentration. The fluorescence of styryl dyes increases after insertion into the hydrophobic environment of the lipid membrane compared their fluorescence in the aqueous phase solution. The alkyl chains of the fluorescent styryl dye probe insert into membranes and are used to understand their biophysical properties and their behavior in lipid bilayers. The mechanism of incorporation of the dyes into cell membranes, or vesicle model systems, is not resolved. In this study we used a modified dialkylaminostyryl fluorescent lipid, 4-(4-(dihexadecylamino)styryl)-N-methylpyridinium iodide (DiA), replacing the I- counterion with the Cl- anion to make DiA-Cl increase hydration of the polar head and to enable self-assembling in water and formation of vesicles. Vesicles composed of DMPC (1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine)/DiA, DPPC (1,2-dipalmitoyl-sn-glycero-3- phosphatidylcholine) /DiA, DSPC (1,2-distearoyl-sn-glycero-3- phosphatidylcholine) /DiA, DMPE (1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine)/DiA, DPPE (1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine)/DiA and DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine)/DiA have been prepared in mole ratios between 100/0 to 0/100, in order to investigate the effects of chain length and headgroup type on chain packing and phase separation in these mixed amphiphilic systems, using nanocalorimetry, dynamic light scattering and fluorescence data, as well as confocal laser scanning microscopy (CLSM) and cryo-transmission electron microscopy (Cryo-TEM). In addition, we report the self-assembly of DiA-Cl, to form H-aggregates of lipid bilayers in aqueous solution, beyond a critical vesicle concentration. Lipid bilayers can be fused onto silica nanoparticles (NPs) to form supported lipid bilayer (SLB)-NPs. (SLB)-NPs have a varous interdisciplinary applications from medicine to environmental fields and agriculture sciences. Here, the lipids on the nanoparticles were used for two applications. One was to adsorb polycyclic aromatic hydrocarbons (PAHs) from the environment and the other was as vehicles for foliar delivery of nutrients to plants. Silica SLB nanoparticles can increase the solubility of Benzo[a]Pyrene (BaP) in order to extract the BaP from soil for in situ biodegradation. Initial studies were begun on the effect of foliar application of silica SLBs nanoparticles on plants. The SLBs to be used were prepared using both 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and DiA, in order to determine whether the lipid increased the entry of the silica into the plant leaves and whether the lipids also entered.
Temple University--Theses
Oldham, Alexis Jean. "Modulation of lipid domain formation in mixed model systems by proteins and peptides". View electronic thesis, 2008. http://dl.uncw.edu/etd/2008-1/r1/oldhama/alexisoldham.pdf.
Pełny tekst źródłaTemprano, López Ana. "The lipin protein family in human adipocytes: lipid metabolism and obesity". Doctoral thesis, Universitat Rovira i Virgili, 2016. http://hdl.handle.net/10803/398025.
Pełny tekst źródłaLas lipinas son una familia de fosfatasas de fosfatidato (PAP1) dependientes de Mg2+ evolutivamente conservadas, que generan diacilglicerol para la síntesis de fosfolípidos y triacilglicerol. En mamíferos, la familia consiste en lipina-1, lipina-2, y lipina-3. Mientras en ratones la mutación del gen Lpin1 causa lipodistrofia, las mutaciones deletéreas en el gen LPIN1 en humanos no afectan a la distribución de grasa. Sin embargo, los individuos con diabetes tipo 2 manifiestan niveles reducidos de expresión de LPIN1 y de actividad PAP1. En esta tesis doctoral se estudia la función de las lipinas en el tejido adiposo humano, la adipogénesis y la lipólisis. Descubrimos que la expresión génica y proteica de las lipinas está alterada en el tejido adiposo de individuos con diabetes tipo 2. La depleción de cada miembro de las lipinas en la línea celular humana de preadipocitos del síndrome Simpson–Golabi–Behmel (SGBS), mostró que, a pesar de que los tres miembros tienen un papel en la adipogénesis temprana, los adipocitos deplecionados de lipinas se diferencian y acumulan lípidos neutros, llevándonos a la hipótesis de la existencia de vías alternativas para la síntesis de triacilglicerol en adipocitos humanos cuando la expresión de las lipinas es reprimida. Las lipinas también intervienen en el reciclaje de los ácidos grasos liberados por la vía lipolítica. Tras la inducción de la lipólisis, las lipinas son defosforiladas y se desplazan a la membrana del retículo endoplásmico, donde ejercen su función. Esta activación es inducida por los ácidos grasos liberados, y revertida con albúmina o triacsin C. La depleción de cada lipina en adipocitos SGBS y posterior inducción de la lipólisis, demuestra su papel en el metabolismo de lípidos neutros. En resumen, las lipinas parecen no tener un papel indispensable en la adipogénesis humana pero sí comprometer el reciclaje de ácidos grasos, importante para la homeostasis lipídica.
Lipins are evolutionarily conserved Mg2+-dependent phosphatidate phosphatases (PAP1) that generate diacylglycerol for phospholipid and triacylglycerol synthesis. In mammals the Lipin family consists of lipin-1, lipin-2 and lipin-3. Whereas mutations in the Lpin1 gene cause lipodystrophy in mouse models, LPIN1 deleterious mutations in humans do not affect fat distribution. However, reduced LPIN1 expression and PAP1 activity have been described in participants with type 2 diabetes. In this doctoral thesis we investigate the roles of all lipin family members in human adipose tissue, adipogenesis and lipolysis. We found that adipose tissue gene and protein expression of the lipin family is altered in type 2 diabetes. Depletion of every lipin family member in a human Simpson–Golabi–Behmel syndrome (SGBS) pre-adipocyte cell line showed that even though all members alter early stages of adipogenesis, lipin-silenced cells differentiate and accumulate neutral lipids, pointing to the hypothesis of alternative pathways for triacylglycerol synthesis under repression of lipin expression. Lipins also have a role in the recycling of the fatty acids released by the lipolytic pathway. They become dephosphorylated upon lipolytic induction, and translocate to their active site, the endoplasmic reticulum membrane. This activation is induced by fatty acids and reversed with albumin or triacsin C. Depletion of every lipin member and subsequently stimulation of lipolysis in SGBS adipocytes revealed a role for lipins in neutral lipid metabolism. Overall, our data support that lipins may not have an indispensable role in adipogenesis, but their depletion compromise fatty acid recycling and lipid homeostasis.
Carr, Neil Owen. "Lipid binding and lipid-protein interaction in wheat flower dough". Thesis, University of Reading, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293285.
Pełny tekst źródłaReeder, Brandon Jon. "Reactions of lipid and lipid hydroperoxides with myoglobin and lipoxygenase". Thesis, University of Essex, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.265191.
Pełny tekst źródłaPERISSINOTTO, FABIO. "Lipid raft formation and lipid-protein interactions in model membranes". Doctoral thesis, Università degli Studi di Trieste, 2018. http://hdl.handle.net/11368/2919798.
Pełny tekst źródłaDanial, John Shokri Hanna. "Imaging lipid phase separation on droplet interface bilayers". Thesis, University of Oxford, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.711943.
Pełny tekst źródłaBlakeston, Anita Catherine. "Biomimetic lipid bilayers". Thesis, University of Leeds, 2015. http://etheses.whiterose.ac.uk/11531/.
Pełny tekst źródłaZhang, Tejia. "Discovery of bioactive lipids and lipid pathways in cell death and disease". Thesis, Harvard University, 2014. http://dissertations.umi.com/gsas.harvard:11483.
Pełny tekst źródłaChemistry and Chemical Biology
Subramaniam, Varuni. "Preparation and Characterization of Novel Lipid and Proteolipid Membranes from Polymerizable Lipids". Diss., The University of Arizona, 2006. http://hdl.handle.net/10150/194889.
Pełny tekst źródłaMcClinchie, Elizabeth A. "Homologs of Mammalian Lysosomal Lipase in Arabidopsis and Their Roles in Lipid Droplet Dynamics". Thesis, University of North Texas, 2017. https://digital.library.unt.edu/ark:/67531/metadc1062826/.
Pełny tekst źródłaGarton, Natalie Jane. "Investigation of mycobacterial lipid domains by use of fluorescent lipid probes". Thesis, University of Newcastle Upon Tyne, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244396.
Pełny tekst źródłaSaeed, Suhur. "Lipid oxidation mechanisms and lipid-protein interactions in frozen mackerel (Scomber scombrus)". Thesis, University of Surrey, 1998. http://epubs.surrey.ac.uk/843251/.
Pełny tekst źródłaBonzom, Pascale Marie Andree. "High resolution NMR applied to lipid analysis and lipid based drug design". Thesis, University College London (University of London), 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.300537.
Pełny tekst źródłaNtola, Chifundo Nyasha Michelle. "Solid lipid matrices for delivery of laundry actives and lipid membrane transport". Thesis, Durham University, 2017. http://etheses.dur.ac.uk/12027/.
Pełny tekst źródłaNikolaus, Jörg. "Hemifusion and lateral lipid domain partition in lipid membranes of different complexity". Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2011. http://dx.doi.org/10.18452/16437.
Pełny tekst źródłaMembrane fusion is ubiquitous in life and requires remodelling of two phospholipid bilayers. Fusion likely proceeds through similar sequential intermediates. A stalk between the contacting leaflets forms and radially expands into a hemifusion diaphragm (HD) wherein finally a fusion pore opens up. Direct experimental verification of this key structure is difficult due to its transient nature. Confocal microscopy was used to visualize the fusion of giant unilamellar vesicles (GUVs) comprising negatively charged phosphatidylserine and fluorescent transmembrane (TM) entities in the presence of divalent cations. A complete displacement of TM peptides preceded full fusion. This is consistent with HD formation. Detailed analysis provided proof that the micrometer sized structures are in fact HDs. HD size is dependent on lipid composition and peptide concentration. Lateral lipid domain formation is believed to be essential for sorting and signalling processes in the cell. Liquid ordered (Lo) domains in model systems like GUVs resemble biological rafts enriched in sphingolipids and cholesterol, but their physical properties seem distinct from biological membranes as judged by e.g. lipid order and packing. In this context the sorting of TM anchored influenza virus hemagglutinin (HA) and different lipid anchored Ras proteins is studied in GUVs and giant plasma membrane derived vesicles (GPMVs). Authentic HA or the TM domain peptides were sorted exclusively (GUVs) or predominantly (GPMVs) to the liquid disordered (Ld) domains. Whereas K-Ras was found in the bulk Ld domains, N-Ras diffuses to the Lo/Ld interface. These results are discussed with respect to differences in lipid packing in the different membrane systems and regarding the membrane anchors and their hydrophobic matching. The results suggest that the formation, size and stability as well as the physical properties of lipid domains in biological membranes are tightly regulated by protein-lipid interactions.
Kamo, Tomoari. "Lipid membrane structure modulated by nonlamellar-forming lipids and interaction with amphipathic peptide". 京都大学 (Kyoto University), 2007. http://hdl.handle.net/2433/137121.
Pełny tekst źródłaCovey, Scott D. Trigatti Bernardo L. "Carrier mediated lipid transport /". *McMaster only, 2003.
Znajdź pełny tekst źródłaHenderson, Erron James. "Lipid peroxidation by myeloperoxidase". Thesis, University of Canterbury. Zoology, 1998. http://hdl.handle.net/10092/6850.
Pełny tekst źródłaBlyth, Alison. "Lipid biomarkers in speleothems". Thesis, University of Newcastle Upon Tyne, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.435638.
Pełny tekst źródłaDaulton, Emma. "Biomimetic floating lipid membranes". Thesis, University of Bath, 2015. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.675722.
Pełny tekst źródłaSherratt, Anna Louise. "Lipid bodies in mycobacteria". Thesis, University of Leicester, 2008. http://hdl.handle.net/2381/30499.
Pełny tekst źródłaKilaru, Aruna. "Lipid Detection and Visualization". Digital Commons @ East Tennessee State University, 2019. https://dc.etsu.edu/etsu-works/7731.
Pełny tekst źródłaMartinez, Ortega Maria Eugenia. "Lipid metal organic networks". To access this resource online via ProQuest Dissertations and Theses @ UTEP, 2009. http://0-proquest.umi.com.lib.utep.edu/login?COPT=REJTPTU0YmImSU5UPTAmVkVSPTI=&clientId=2515.
Pełny tekst źródłaThorén, Klas. "Lipid-extracted bone grafts". Lund : Dept. of Orthopedics, University Hospital, Lund University, 1994. http://catalog.hathitrust.org/api/volumes/oclc/39676934.html.
Pełny tekst źródłaDarroch, Peter Ian. "Lipid phosphate phosphatases : purification and investigation of their role in cellular lipid signalling". Thesis, University of Strathclyde, 2001. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=21171.
Pełny tekst źródłaDing, Yuan [Verfasser]. "Lipid nanoparticles for topical delivery: solid lipid nanoparticles (SLN) & smartLipids / Yuan Ding". Berlin : Freie Universität Berlin, 2018. http://d-nb.info/1176632329/34.
Pełny tekst źródłaBorthwick, Faye. "Studies on the role of 'start' lipid trafficking proteins in macrophage lipid homeostasis". Thesis, Glasgow Caledonian University, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.496152.
Pełny tekst źródłaNg, Ai-Leng. "Comparisons of serum lipid levels and dietary lipid intakes of parents and children". Thesis, Virginia Tech, 1988. http://hdl.handle.net/10919/43259.
Pełny tekst źródłaMaster of Science
Addy, Victoria Louise. "The use of lipid hydrolases to target the lipid components of ovine skin". Thesis, University of Oxford, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.711595.
Pełny tekst źródłaVeatch, Sarah Louise. "Liquid immiscibility in model bilayer lipid membranes /". Thesis, Connect to this title online; UW restricted, 2004. http://hdl.handle.net/1773/9772.
Pełny tekst źródłaSturgeon, Raymond M. "Interplay between cations, anionic lipids, and lipid-protein interactions at the nicotinic acetylcholine receptor". Thesis, University of Ottawa (Canada), 2009. http://hdl.handle.net/10393/28070.
Pełny tekst źródłaPrindiville, John S. "Circulating lipoproteins and tissue lipids: Effects of gemfibrozil on lipid metabolism in rainbow trout". Thesis, University of Ottawa (Canada), 2010. http://hdl.handle.net/10393/28555.
Pełny tekst źródłaHerzog, Ronny. "Novel concepts for lipid identification from shotgun mass spectra using a customized query language". Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2012. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-90497.
Pełny tekst źródłaMateos, Diaz Eduardo. "Etude par spectroscopie infrarouge (FTIR) des interactions de la lipase pancréatique apparentée de type 2 (PLRP2) avec les phospholipides et les sels biliaires". Thesis, Aix-Marseille, 2016. http://www.theses.fr/2016AIXM4763.
Pełny tekst źródłaGuinea pig pancreatic lipase-related protein type 2 (GPLRP2) hydrolyzes a large set of lipid substrates, but displays however some selectivity depending on the supramolecular structure of substrate and the presence of surfactants like bile salts (NaTDC). We used Fourier transform infrared (FTIR) spectroscopy to study the interactions between phospholipids (DPPC), surfactants and GPLRP2 under conditions close to those of the GI tract. To study the adsorption step independently from hydrolysis, a GPLRP2 inactive variant (S152G) was produced. Various phospholipid dispersions were prepared: multilamellar (MLV) and large unilamellar vesicles (LUV) and mixed micelles with surfactants. GPLRP2 was found to hydrolyze DPPC present in mixed DPPC-NaTDC micelles but was inactive on DPPC vesicles and DPPC-Triton X100 micelles. FTIR analysis of GPLRP2 S152G interaction with the DPPC-NaTDC system showed a decrease in the conformational disorder and mobility of the acyl chains, a dehydratation of the interface, and changes in the orientation and H-bonding of DPPC polar head-groups. These effects were not observed with MLV, LUV and DPPC-Triton X100 micelles, thus indicating a specific recognition of DPPC in mixed phospholipid-bile salt micelles, in agreement with phospholipase activity measurements. Changes in the IR spectra during DPPC hydrolysis by GPLRP2 were monitored. Specific spectral features were associated to the production of lipolysis products and could be used for quantifying phospholipid lipolysis by FTIR
Petkevicius, Kasparas. "The role of macrophage intracellular lipid partitioning in glucose and lipid homeostasis during obesity". Thesis, University of Cambridge, 2019. https://www.repository.cam.ac.uk/handle/1810/285429.
Pełny tekst źródłaCheong, Fei Ying. "Regulation of lipid signaling at the Golgi by the lipid phosphatases hSAC1 and OCRL1". [S.l. : s.n.], 2007. http://nbn-resolving.de/urn:nbn:de:bsz:16-opus-71011.
Pełny tekst źródłaTurenne, Eric D. "Lipid Mobilization In Exercising Salmonids". Thesis, Université d'Ottawa / University of Ottawa, 2018. http://hdl.handle.net/10393/37075.
Pełny tekst źródłaHarczy, Martha. "Lipid mediators in lung anaphylaxis". Mémoire, Université de Sherbrooke, 1988. http://hdl.handle.net/11143/11716.
Pełny tekst źródłaShilton, Catherine Margaret. "Corneal lipid deposition in anurans". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0020/NQ55639.pdf.
Pełny tekst źródłaHafez, Ismail Mahmoud. "Lipid polymorphism and intracellular delivery". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0021/NQ56555.pdf.
Pełny tekst źródłaRaggers, René John. "Lipid translocation by multidrug transporters". [S.l. : Amsterdam : s.n.] ; Universiteit van Amsterdam [Host], 2001. http://dare.uva.nl/document/60218.
Pełny tekst źródłaDerrien, Thomas. "Gold nanoparticle-lipid bilayer interactions". Thesis, McGill University, 2010. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=86727.
Pełny tekst źródłaL'interaction des nanoparticules d'or avec les bicouches lipidiques est présentée dans ce mémoire. Les facteurs influençant cette interaction ont été explorés en utilisant des bicouches lipidiques synthétiques. L'interaction due à l'incorporation des nanoparticules au sein des bicouches a été étudiée par des techniques d'imagerie. Un test de fuite de fluorophore a été employé afin de déterminer l'influence de la composition et de la structure des ligands protégeant les nanoparticules sur leur incorporation dans les bicouches de lipides. Pour cela, nous avons développer une synthèse de nanoparticules protégées par deux types de ligands. Des expériences in vivo ont été réalises avec des nanoparticules d'or fonctionnalisées avec des peptides ainsi que des fluorophores, mis en contact avec des cellules vivantes de type HeLa. Nous avons constaté que les nanoparticules d'or sont capables de franchir les bicouches lipidiques en utilisant des mécanismes indépendants d'énergie. Nous concluons que la structure et la composition des ligands protégeant les nanoparticules ont une grande influence sur la perturbation qu'elles induisent dans la structure des bicouches lipidiques.
Khatchadourian, Armen. "Lipid droplets under stressful conditions". Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=116901.
Pełny tekst źródłaLes gouttelettes lipidiques (GL) sont des organites phylogénétiquement conservées et impliquées dans plusieurs fonctions cellulaires. Durant les deux dernières décennies, notre compréhension des rôles biologiques et physiologiques des GL a augmenté de manière draconienne. Plusieurs observations suggèrent fortement que les GL jouent un rôle important dans l'inflammation, ainsi que dans les désordres métaboliques tels que le diabète de type 2 (DT2). Malgré cette avancée, plusieurs aspects de la biologie des GL et de leurs rôles dans des maladies demeurent méconnus.Le centre des GL est riche en lipides neutres qui peuvent se mobiliser et servir comme source d'énergie. La couche phospholipidique entourant le centre de la GL est associée à plusieurs protéines et enzymes métaboliques. Bien que les GL puissent être induites par des acides gras, elles peuvent aussi l'être dans des conditions de stress. Par contre, les mécanismes de l'accumulation de GL par des conditions de stress ne sont pas encore bien compris. Notre objectif principal est de comprendre la régulation de la formation de GL par le stress oxydatif, l'inflammation et le stress métabolique. Premièrement, nous avons investigué les GL dans des cellules exposées à des stresseurs tels que des nanocrystaux métalliques et des dérivés réactifs d'oxygène. La formation de GL et l'expression de perilipin-2, qui est une protéine structurelle des GL, ont tous deux augmenté dans les cellules stressées. De plus, une supplémentation en antioxydant (n-acétylcystéine) ou un traitement avec un inhibiteur de p38 MAPK a réduit l'accumulation de GL causée par le stress. Ces observations suggèrent que le stress oxydatif et p38 MAPK jouent un rôle dans l'accumulation de GL dans des cellules stressées. Il est bien connu que les leucocytes et macrophages qui sont engagés dans l'inflammation contiennent une grande quantité de GL. Même si ce phénomène a bien été exploré dans les cellules immunitaires périphériques, il reste inexploré dans le système nerveux central (SNC). Ce faisant, nous avons investigué la dynamique et la régulation des GL dans les microglies, les cellules résidentes immunitaires dans le cerveau. Nous avons trouvé que dans les microglies stimulées avec les lipopolysaccharides (LPS), les GL et l'expression de perilipin-2 ont augmenté d'une manière dépendante de l'activation de l'Akt et p38 MAPK. Dans ces cellules activées, la phospholipase cytosolique A2-α (PLC A2-α), une enzyme fonctionnant dans la synthèse d'éicosanoides, des médiateurs lipidiques inflammatoires, colocalisait avec les GL. Ensemble, ces résultats indiquent que la formation de GL pourrait contribuer à la synthèse d'éicosanoides dans les microglies activées et servir de biomarqueurs d'inflammation dans le SNC.Pour mieux comprendre le rôle des GL dans la pathologie humaine, nous les avons examinées dans des tissues pancréatiques provenant de patients obèses ou diabétiques T2. Nos études immunohistochimiques ont révélé une augmentation de perilipin-2 dans les îlots de Langerhans chez les patients diabétiques obèses ou maigres, mais pas dans ceux de patients non-diabétiques. Ceci suggère que le DT2, mais non l'obésité, est requis pour une augmentation de perilipin-2 dans le pancréas. L'analyse d'expression de gènes par RT-PCR a confirmé l'augmentation de perilipin-2 observé antérieurement dans les îlots et a également révélé des altérations dans des gènes reliés aux fonctions des îlots, au métabolisme, et aux défenses anti-oxydantes. Ces changements, qui sont souvent associés à l'obésité et au DT2, constituent un mécanisme d'adaptation à la résistance à l'insuline et au stress métabolique.Pour résumer, nos études démontrent que l'accumulation de GL fait partie intégrante de l'adaptation des cellules au stress. Durant la prochaine décennie, le plus grand obstacle dans la recherche sur les GL sera de déterminer comment la composition lipidique ou protéinique de ces organites affecte leurs fonctions biologiques.
McEwan, S. J. "Studies on skeletal lipid metabolism". Thesis, University of Oxford, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233475.
Pełny tekst źródłaDeol, Sundeep Singh. "Analysis of lipid-protein interactions". Thesis, University of Oxford, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.424760.
Pełny tekst źródła