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Kulikova, Nataliya Gennadievna, Zaur Gidovich Zhilokov, Albina Sergeevna Tkachenko i Tinatin Chkheidze. "On the use of laser therapy in dentistry". Fizioterapevt (Physiotherapist), nr 01 (13.01.2022): 33–40. http://dx.doi.org/10.33920/med-14-2202-04.
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The aim of the research: to optimize the prevention of dental complications by using electromagnetic low-intensity laser radiation (LILR) techniques on dental and periodontal tissues. Research methods. In 138 dental patients, a clinical and functional assessment of the dental apparatus was carried out, dental indices were studied, the results of vascular endothelial parameters (VEGF-A, sVEGF-R1, sVEGF-R2) and immune parameters (FNF, TNF, IFN-γ, neutrophils) of blood serum (solid-phase enzyme immunoassay with a standard set), as well as VAS pain syndrome parameters were analyzed before/after laser therapy of different wavelength, mode and range (red, infrared). Results. The study made it possible to assess the effectiveness of treatment with LILR with one wavelength and in combination of different lengths of laser radiation as independent factors of preventive exposure in dentistry patients. After applying the techniques of transcanal treatment, dental periapical tissues were treated with laser radiation, including root canals (difficult teeth roots), using the C11 attachment from the «Lazmik-01» device. The study used a modulated red spectrum of LILR in continuous and pulsed mode (l = 635 nm) and an infrared pulsed spectrum (l = 904 nm) in a continuous mode with a sequential exposure to red (l = 635 nm) pulsed and infrared continuous laser radiation (l = 904 nm). Emphasis was placed on pain syndrome, vascular endothelial and immune shifts in dental and periodontal tissues before/after using laser therapy in dental patients. Conclusion. The combination of the red spectrum of LILR in pulsed mode (l = 635 nm) with a sequential exposure to infrared laser radiation (l = 904 nm) in continuous mode provides high vascular endothelial responses in the dental and periodontal tissues of the dental apparatus, and the use of red-spectrum laser radiation in continuous mode (l = 635 nm) in dental patients provides antimicrobial and sanitizing effects, including at the level of the root canals, which is more significant than after the use of infrared laser radiation in pulsed mode, when analgesic results are higher.
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Макаров, С. С., С. А. Родин, И. Б. Кузнецова, Г. Ю. Макеева i В. А. Макеев. "Rooting in vitro Culture and Adaptation of Marsh Cranberry (Oxycoccus palustris Pers.) to Non-sterile Conditions". Лесохозяйственная информация, nr 4 (17.12.2020): 105–14. http://dx.doi.org/10.24419/lhi.2304-3083.2020.4.11.
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В статье приводятся данные по процессу корнеобразования клюквы болотной в культуре in vitro и ее адаптации к нестерильным условиям. Выявлено благоприятное влияние добавления в питательную среду препарата Экогель в концентрации 0,5 мг/л на процесс ризогенеза клюквы болотной in vitro. Установлена более высокая приживаемость растений при адаптации растений к нестерильным условиям in vivo на субстрате из верхового торфа. One of the most valuable forest crops is marsh cranberry (Oxycoccus palustris Pers.) The article presents data on the adaptation of marsh cranberries to non-sterile conditions, as well as the effect of BMI auxin and Ecogel at the stage of plant rooting in vitro. The research was conducted on the variety of marsh cranberry Dar Kostroma and hybrid 1-15-635. As a result of research, it was found that with an increase in the nutrient medium concentration of AUXIN BMI from 0.5 to 1.0 mg/l, the number of marsh cranberry roots increased on average in the Dar Kostroma variety from 2.4 to 2.7 PCs, and in the hybrid 1-15-635 from 2.1 to 2.4 PCs. When ecogel was added to the Nutrient medium at a concentration of 0.5 mg/l, the number of roots on average reached 3.1 PCs, and without Ecogel it was only 1.7 PCs. It was also noted that the average length of marsh cranberry roots with an increase in the concentration of BMI auxin and the presence of Ecogel in the nutrient medium increased in the Dar Kostroma variety from 1.5 to 2.0 cm, in the 1-15-635 hybrid from 1.3 to 1.5 cm, while without Ecogel it decreased from 1.0 to 0.9 cm and from 1.1 to 0.8 cm, respectively. Adding Ecogel to the nutrient medium at a concentration of 0.5 mg/l increased the average length of cranberry roots by an average of 1.8 times. The total length of marsh cranberry roots, with an increase in the concentration of BMI auxin and the presence of Ecogel in the nutrient medium at a concentration of 0.5 mg/l, increased in the Dar Kostroma variety from 5.1 to 6.0 cm, and in the hybrid 1-15-635 from 4.1 to 4.4 cm, without Ecogel – decreased from 2.6-3.1 to 2.5 cm.
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K.S., Reshetnyk. "Вплив лазерного випромінювання та концентарції глюкози на вміст каротиноїдів у міцелії грибів Laetiporus sulphureus (Bull.) Murrill". Chornomorski Botanical Journal 16, nr 4 (11.01.2021): 333–42. http://dx.doi.org/10.32999/ksu1990-553x/2020-16-4-6.
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У статті представлено результати дослідження кількості каротиноїдів міцелію L. sulphureus за дії LED лазерів: BRP–3010–5, з випромінюванням червоного спектру з довжиною хвилі 635 нм, BBP–3010–5 з випромінюванням синього спектру з довжиною хвилі 405 нм та BGP–3010–5 з випромінюванням зеленого спектру з довжиною хвилі 532 нм (енергія опромінення 51,1 мДж/см2 ) при культивуванні на живильному середовищі з різними концентраціями глюкози. Контролем неопромінений міцелій. Встановлено, що найефективнішим для синтезу каротиноїдів є використання глюкозо-пептонного середовища з концентрацією глюкози 10 г/дм3 у комплексі з опроміненням міцелію зеленим світлом довжиною хвилі 532 нм (енергія опромінення 51,1 мДж/см2 ). За дії цього режиму опромінення для штаму L.s.-18 вміст каротиноїдів у міцелії зріс на 66,1 % відповідно до контролю. Лазерне опромінення міцелію синім світлом довжиною хвилі 405 нм (енергія опромінення 51,1 мДж/см2 ) збільшило кількість каротиноїдів для штаму L.s.-18 на 46,7 %. Опромінення червоним світлом довжиною хвилі 635 нм (енергія опромінення 51,1 мДж/см2 ) сприяло зростанню кількості каротиноїдів для штаму L.s.-16 гриба L. sulphureus на 28,9 %. Встановлено, що використання глюкозо-пептонного середовища з концентрацією глюкози 8 г/дм3 у комплексі з опроміненням міцелію зеленим світлом довжиною хвилі 532 нм (енергія опромінення 51,1 мДж/см2 ) було менш ефективним. За цих умов вміст каротиноїдів у міцелію зріс для штаму L.s.-17 на 62,3%. Лазерне опромінення міцелію синім світлом довжиною хвилі 405 нм (енергія опромінення 51,1 мДж/см2 ) збільшило кількість каротиноїдів для штаму L.s.-17 на 30,6% відповідно. Опромінення червоним світлом довжиною хвилі 635 нм (енергія опромінення 51,1 мДж/см2 ) сприяло зростанню кількості каротиноїдів для штаму Ls18 гриба L. sulphureus на 16,8% відповідно. Для штаму L.s.-16 кількість каротиноїдів у міцелії не зросла. Під час використання глюкозо-пептонного середовища концентраціями глюкози 6 та 4 г/дм3 у комплексі з лазерним опромінення міцелію червоним (довжина хвилі 635 нм), синім (довжина хвилі 405 нм) та зеленим (довжина хвилі 532 нм) світлом з енергією опромінення 51,1 мДж/см2 не відбувалося зростання вмісту каротиноїдів у міцелії. Ключові слова: міцелій, каротиноїди, фоторецепція, фотоактивація
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K.S., Reshetnyk. "Вплив лазерного випромінювання та концентарції глюкози на вміст каротиноїдів у міцелії грибів Laetiporus sulphureus (Bull.) Murrill". Chornomorski Botanical Journal 16, nr 4 (11.01.2021): 333–42. http://dx.doi.org/10.32999/ksu1990-553x/2020-16-4-6.
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У статті представлено результати дослідження кількості каротиноїдів міцелію L. sulphureus за дії LED лазерів: BRP–3010–5, з випромінюванням червоного спектру з довжиною хвилі 635 нм, BBP–3010–5 з випромінюванням синього спектру з довжиною хвилі 405 нм та BGP–3010–5 з випромінюванням зеленого спектру з довжиною хвилі 532 нм (енергія опромінення 51,1 мДж/см2 ) при культивуванні на живильному середовищі з різними концентраціями глюкози. Контролем неопромінений міцелій. Встановлено, що найефективнішим для синтезу каротиноїдів є використання глюкозо-пептонного середовища з концентрацією глюкози 10 г/дм3 у комплексі з опроміненням міцелію зеленим світлом довжиною хвилі 532 нм (енергія опромінення 51,1 мДж/см2 ). За дії цього режиму опромінення для штаму L.s.-18 вміст каротиноїдів у міцелії зріс на 66,1 % відповідно до контролю. Лазерне опромінення міцелію синім світлом довжиною хвилі 405 нм (енергія опромінення 51,1 мДж/см2 ) збільшило кількість каротиноїдів для штаму L.s.-18 на 46,7 %. Опромінення червоним світлом довжиною хвилі 635 нм (енергія опромінення 51,1 мДж/см2 ) сприяло зростанню кількості каротиноїдів для штаму L.s.-16 гриба L. sulphureus на 28,9 %. Встановлено, що використання глюкозо-пептонного середовища з концентрацією глюкози 8 г/дм3 у комплексі з опроміненням міцелію зеленим світлом довжиною хвилі 532 нм (енергія опромінення 51,1 мДж/см2 ) було менш ефективним. За цих умов вміст каротиноїдів у міцелію зріс для штаму L.s.-17 на 62,3%. Лазерне опромінення міцелію синім світлом довжиною хвилі 405 нм (енергія опромінення 51,1 мДж/см2 ) збільшило кількість каротиноїдів для штаму L.s.-17 на 30,6% відповідно. Опромінення червоним світлом довжиною хвилі 635 нм (енергія опромінення 51,1 мДж/см2 ) сприяло зростанню кількості каротиноїдів для штаму Ls18 гриба L. sulphureus на 16,8% відповідно. Для штаму L.s.-16 кількість каротиноїдів у міцелії не зросла. Під час використання глюкозо-пептонного середовища концентраціями глюкози 6 та 4 г/дм3 у комплексі з лазерним опромінення міцелію червоним (довжина хвилі 635 нм), синім (довжина хвилі 405 нм) та зеленим (довжина хвилі 532 нм) світлом з енергією опромінення 51,1 мДж/см2 не відбувалося зростання вмісту каротиноїдів у міцелії. Ключові слова: міцелій, каротиноїди, фоторецепція, фотоактивація
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RESHETNYK, KATERYNA SERGIIVNA. "The influence of laser irradiation and glucose concentration on the content of carotenoids in the mycelium of fungus Laetiporus sulphureus (Bull.) Murrill". CHORNOMORSKI BOTANICAL JOURNAL 16, nr 4 (30.11.2020): 333–42. http://dx.doi.org/10.32999/ksu1990553x/2020-16-4-6.
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The article presents the results of the study of the content of carotenoids of L. sulphureus mycelium under the action of LED lasers: BRP–3010–5, with red spectrum radiation with a wavelength of 635 nm, BBP–3010–5 with blue spectrum radiation with a wavelength of 405 nm and BGP–3010–5 with green spectrum radiation with a wavelength of 532 nm (irradiation energy 51.1 mJ/cm2) when cultured on nutrient medium with different concentrations of glucose. The irradiated mycelium served as a control. It was found that is most effective for the synthesis of carotenoids the use of glucose-peptone medium with a glucose concentration of 10 g/dm³ in combination with irradiation of mycelium with green light at a wavelength of 532 nm (irradiation energy 51.1 mJ/cm2). Under the action of this irradiation regime for strain L.s.-18 the content of carotenoids in the mycelium increased by 66.1% according to the control. Laser irradiation of mycelium with blue light with a wavelength of 405 nm (irradiation energy 51.1 mJ/cm2) increased the content of carotenoids for strain L.s.-18 by 46.7%. Irradiation with red light with a wavelength of 635 nm (irradiation energy 51.1 mJ/cm2) contributed to an increase in the content of carotenoids for strain L.s.-16 of the fungus L. sulphureus by 28.9%. It was found that the use of glucose-peptone medium with a glucose concentration of 8 g/dm3 in combination with irradiation of the mycelium with green light with a wavelength of 532 nm (irradiation energy 51.1 mJ/cm2) was less effective. Under these conditions, the content of carotenoids in the mycelium increased for strain L.s.-17 by 62.3%. Laser irradiation of mycelium with blue light with a wavelength of 405 nm (irradiation energy 51.1 mJ/cm2) increased the content of carotenoids for strain L.s.-17 by 30.6%. Irradiation with red light with a wavelength of 635 nm (irradiation energy 51.1 mJ/cm2) contributed to an increase in the content of carotenoids for strain L.s.-18 of the fungus L. sulphureus by 16.8% respectively. For strain L.s.-16 the number of carotenoids in the mycelium no increase. The use glucose-peptone medium with glucose concentrations of 6 and 4 g/dm3 in combination with laser irradiation of mycelium with red (wavelength 635 nm), blue (wavelength 405 nm) and green (wavelength 532 nm) light with irradiation energy 51.1 mJ/cm2 was no increase in the content of carotenoids in the mycelium.
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Al-Araji, Kadhim Hashim Yaseen. "Evaluation of Physical Chemical and Biological Characteristics of Underground Wells in Badra City, Iraq". Baghdad Science Journal 16, nr 3 (1.09.2019): 0560. http://dx.doi.org/10.21123/bsj.2019.16.3.0560.
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The quality of groundwater should be improved by keeping safe water sources from contaminants in protective way by doing regular measuring and checkup before it supplied for usage. Private Wells do not receive the same services that wells supplying the public do. Well owners are responsible for protecting their drinking water. This work was carried out in Badra city, Iraq from December 2017 to May 2018, six wells water were investigated to determine the general characteristics of wells as well as studying the effect of environmental factors on the quality of water. The average of six wells were eleven parameters that is out of permissible limits were EC, Sal., Alk., TH, TDS, Na, Ca, Cl, SO4, Fe, Zn (4402-5183 /cm, 2.76-3.9 ppt, 302-366mg/L, 3164-4248 mg/L, 604-675 mg/L, 375-524 mg/L, 635-871 mg/L, 631-1107 mg/L, 2430-4570 g/L, 114-392 g/L). Respectively, microbiological investigations involved the total coliform, total plate count, as well as the detection for the presence of E. coli, Salmonella and Cholera. Results shows that there is a significant relation between the increasing of the TDS and Turbidity, TDS gives an indication for the significant increasing of chemical ions. Wells number 3, 4 and 5 showed gave positive results for E.coli growth which as a source of microbial pollution. Detection for the presence of chemical and microbial contaminate is an important alarm since this water has a direct effect on the human and animal's health. Advance method of rapid detection for the well water quality is highly recommended to avoid any health issue and prevent the outbreak of health risk and ecological contaminants.
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Bharat, Rajeev, Vikas Gupta, Meenakshi Gupta i SK Rai. "Effects of Different Sowing Schedules and Planting Geometry on Yield and Productivity of Brassica Juncea L." Bangladesh Journal of Botany 51, nr 3 (3.10.2022): 631–35. http://dx.doi.org/10.3329/bjb.v51i3.62011.
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A field experiment was conducted to study the effects of different sowing schedules and planting geometry on yield and productivity of Indian mustard (Brassica juncea L.) var. Giriraj. Among the different sowing schedules, sowing mustard crop on 2nd decade of October was found to produce significantly higher seed and biological yield. The early sowing of mustard crop recorded higher values of utilization and accumulation of various agromet indices like growing degree days (GDD), helio-thermal units (HTU) and photo-thermal units (PTU) as well as higher values of photothermal index (PTI) at different phenological stages than other treatments in comparison. Among the planting geometry significant increase in the seed and biological yield was observed in the plots sown with planting geometry of 30 cm × 10 cm which however was found to be at par with 30 cm × 20 cm. The maximum and minimum temperature and thermal indices at vegetative and reproductive stages had a significant correlation on seed yield of mustard crop during the three years of experimentation. Similarly, heat use efficiency was also significantly related with seed and biological yield of mustard. Bangladesh J. Bot. 51(3): 631-635, 2022 (September)
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Shimamoto, M., S. Takewaki, S. Sakuraoka, T. Nagasawa, K. Kuroiwa, O. Kodama i T. Akatsuka. "Use of L-leucyl-3-carboxy-4-hydroxyanilide as substrate for determining the activity of microsomal aminopeptidase in serum." Clinical Chemistry 31, nr 10 (1.10.1985): 1636–39. http://dx.doi.org/10.1093/clinchem/31.10.1636.
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Abstract We describe a colorimetric method for assay of microsomal aminopeptidase (EC 3.4.11.2) activity in serum. We use a new substrate, L-leucyl-3-carboxy-4-hydroxyanilide, p-xylenol as coupler, and sodium metaperiodate as oxidizing reagent. The colored substance formed by the oxidative condensation between p-xylenol and 5-aminosalicylic acid absorbs maximally at 635 nm, and can be directly measured in serum. In a previous method for this enzyme, L-leucyl-beta-naphthylamide was used as substrate and beta-naphthylamine, a carcinogenic reagent used as a standard in making the assay, was unsuitable for routine use. We found a close correlation between results obtained with the new, safer method and with the previous method.
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Yang, Cheng, Linda H. Shapiro, Morris Rivera, Alok Kumar i Paul K. Brindle. "A Role for CREB Binding Protein and p300 Transcriptional Coactivators in Ets-1 Transactivation Functions". Molecular and Cellular Biology 18, nr 4 (1.04.1998): 2218–29. http://dx.doi.org/10.1128/mcb.18.4.2218.
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ABSTRACT The Ets-1 transcription factor plays a critical role in cell growth and development, but the means by which it activates transcription are still unclear (J. C. Bories, D. M. Willerford, D. Grevin, L. Davidson, A. Camus, P. Martin, D. Stehelin, F. W. Alt, and J. C. Borles, Nature 377:635–638, 1995; N. Muthusamy, K. Barton, and J. M. Leiden, Nature 377:639–642, 1995). Here we show that Ets-1 binds the transcriptional coactivators CREB binding protein (CBP) and the related p300 protein (together referred to as CBP/p300) and that this interaction is required for specific Ets-1 transactivation functions. The Ets-1- and c-Myb-dependent aminopeptidase N (CD13/APN) promoter and an Ets-1-dependent artificial promoter were repressed by adenovirus E1A, a CBP/p300-specific inhibitor. Furthermore, Ets-1 activity was potentiated by CBP and p300 overexpression. The transactivation function of Ets-1 correlated with its ability to bind an N-terminal cysteine- and histidine-rich region spanning CBP residues 313 to 452. Ets-1 also bound a second cysteine- and histidine-rich region of CBP, between residues 1449 and 1892. Both Ets-1 and CBP/p300 formed a stable immunoprecipitable nuclear complex, independent of DNA binding. This Ets-1–CBP/p300 immunocomplex possessed histone acetyltransferase activity, consistent with previous findings that CBP/p300 is associated with such enzyme activity. Our results indicate that CBP/p300 may mediate antagonistic and synergistic interactions between Ets-1 and other transcription factors that use CBP/p300 as a coactivator, including c-Myb and AP-1.
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Макаров, С. С., И. Б. Кузнецова, Г. Ю. Макеева i В. А. Макеев. "Influence of Light on the Organogenesis of the European Cranberry (Oxycoccus palustris Pers.) during Clonal Micropropagation". Лесохозяйственная информация, nr 2 (3.06.2021): 106–15. http://dx.doi.org/10.24419/lhi.2304-3083.2021.2.09.
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Приведены результаты исследований по влиянию освещения разного спектрального диапазона на органогенез растений клюквы болотной сорта Дар Костромы и перспективной гибридной формы 1-15-635 при клональном микроразмножении на этапах «собственно микроразмножение» и «укоренение in vitro». Растения-регенеранты культивировали на питательной среде WPM с добавлением на этапе «собственно микроразмножение» цитокинина 2ip 0,5 мг/л, на этапе «укоренение in vitro» – ауксина ИМК 0,5 мг/л. Для освещения использовали светодиодные лампы белого спектра и с комбинацией белого и красного спектров, а также люминесцентные лампы белого цвета. Выявлено значительное увеличение биометрических показателей растений клюквы болотной in vitro при освещении светодиодными лампами с комбинацией белого и красного спектров: количество микропобегов было почти в 1,9 раза больше, а их суммарная длина – в 4,0–4,2 раза больше, чем при освещении лампами белого спектра. Более мощное развитие надземной части растений клюквы болотной способствовало более интенсивному развитию корневой системы. При освещении надземной части растений лампами с комбинацией белого и красного спектров количество корней было в 1,8–2,5 раза больше, а их суммарная длина – в 2,4–3,5 раза больше, чем при освещении лампами белого спектра. Растения клюквы болотной гибридной формы 1-15-635 формировали более мощные надземную часть и корневую систему, чем у растений сорта Дар Костромы. The results of studies on the effect of illumination of different spectral ranges on the organogenesis of marsh cranberry plants of the Dar Kostroma cultivarand the promising hybrid form 1-15-635 during clonal micropropagation at the stages of “proper micropropagation” and in vitro rooting. Regenerated plants cultivated on a WPM nutrient medium with addition of 2ip 0.5 mg/l at the micropropagation stage, and IMC 0.5 mg/l at the in vitro rooting stage. LED lamps of a white spectrum and with a combination of white and red spectra, as well as white fluorescent lamps are used for lighting. A significant increase in biometric parameters of marsh cranberry plants in vitro is revealed when illuminated with LED lamps with a combination of white and red spectra. The number of microshoots of marsh cranberry under illumination of regenerant plants with LED lamps with a combination of white and red spectra is almost 1.9 times greater, and the total length is 4.0–4.2 times greater than under illumination with white spectrum lamps. More powerful development of the aboveground part of the marsh cranberry plants contributed to the more intensive development of the root system. The number of roots was 1.8–2.5 times greater, and the total length was 2.4–3.5 times greater when the aboveground part of the plants is illuminated with lamps with a combination of white and red spectra than with white lamps. A more powerful aboveground part and root system are formed in marsh cranberry plants of hybrid form 1-15-635 than the Dar Kostroma cultivar.
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Frazão, Paulo, Marco A. Peres i Jaime A. Cury. "Qualidade da água para consumo humano e concentração de fluoreto". Revista de Saúde Pública 45, nr 5 (październik 2011): 964–73. http://dx.doi.org/10.1590/s0034-89102011005000046.
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O artigo visa analisar a concentração de fluoreto na água para consumo humano, considerando o balanço entre benefícios e riscos à saúde, e produzir subsídios para atualização da legislação brasileira. Estudos de revisão sistemática, documentos oficiais e dados meteorológicos foram examinados. As temperaturas nas capitais brasileiras indicam que o fluoreto deveria variar de 0,6 a 0,9 mg/L para prevenir cárie dentária. Concentração de fluoreto natural de 1,5 mg/L é tolerável para consumo no Brasil se não houver tecnologia de custo-benefício aceitável para ajuste/remoção do seu excesso. A ingestão diária de água com fluoreto em concentração > 0,9 mg/L representa risco à dentição em menores de oito anos de idade e os consumidores deveriam ser expressamente informados desse risco. Considerando a expansão do programa nacional de fluoretação da água para regiões de clima tipicamente tropical, deve-se revisar a Portaria 635/75, relacionada ao fluoreto adicionado às águas de abastecimento público.
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Maheux, Andrée F., Vanessa Dion-Dupont, Sébastien Bouchard, Marc-Antoine Bisson, Michel G. Bergeron i Manuel J. Rodriguez. "Comparison of four β-glucuronidase and β-galactosidase-based commercial culture methods used to detect Escherichia coli and total coliforms in water". Journal of Water and Health 13, nr 2 (9.10.2014): 340–52. http://dx.doi.org/10.2166/wh.2014.175.
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The MI agar, Colilert®, Chromocult coliform® agar, and DC with BCIG agar chromogenic culture-based methods used to assess microbiological quality of drinking water were compared in terms of their ubiquity, sensitivity, ease of use, growth of atypical colonies and affordability. For ubiquity, 129 total coliform (representing 76 species) and 19 Escherichia coli strains were tested. Then, 635 1-L well water samples were divided into 100 mL subsamples for testing by all four methods. Test results showed that 70.5, 52.7, 36.4, and 23.3% of the non-E. coli total coliform strains and 94.7, 94.7, 89.5, and 89.5% of the 19 E. coli strains yielded a positive signal with the four methods, respectively. They also yielded a total coliform positive signal for 66.5, 51.7, 64.9, and 55.0% and an E. coli positive signal for 16.1, 14.8, 17.3, and 13.4% of the 635 well water samples tested, respectively. Results showed that Colilert® is the most expensive method tested in terms of reactants, yet it is the easiest to use. Large numbers of atypical colonies were also often observed on Chromocult coliform® and DC with BCIG, thereby challenging the target microorganism count. Thus, the MI agar method seems to be the best option for the assessment of drinking water quality.
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Макаров, С. С., П. А. Феклистов, Т. А. Макарова, И. Б. Кузнецова i З. А. Самойленко. "Influence of the Сoncentration of Growth-Regulating Preparation on the Biometric Indicators of the European Cranberry (Oxycoccus palustris Pers.) during in vitro Cultivation". Лесохозяйственная информация, nr 2 (14.06.2022): 47–55. http://dx.doi.org/10.24419/lhi.2304-3083.2022.2.04.
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Приведены результаты исследований по выращиванию клюквы болотной (Oxyccocus palustris Pers.) сорта Дар Костромы и гибридной формы 1-15-635 в культуре in vitro на этапе «собственно микроразмножение» на питательной среде WPM. Клональное микроразмножение наиболее целесообразно использовать для ускоренного получения большого количества высококачественного посадочного материала лесных ягодных растений. Однако технологии клонального микроразмножения клюквы болотной сортов российской селекции нуждаются в совершенствовании. В качестве росторегулирующих веществ использовали цитокинин 2-iP в концентрациях 1,0 и 2,0 мг/л и адаптоген Эпин-Экстра в концентрации 0,5 мл/л. При повышении концентрации цитокинина 2-iP от 1,0 до 2,0 мг/л в питательной среде WPM возросли количество (в 1,2 раза) и суммарная длина (в 1,3 раза) микропобегов клюквы болотной in vitro. Добавление препарата Эпин-Экстра 0,5 мг/л в питательную среду способствовало увеличению количества (в 1,1 раза) и суммарной длины (в 1,2 раза) микропобегов. Существенных различий по количеству и длине микропобегов клюквы болотной в зависимости от сорта или формы не выявлено. The results of studies on the cultivation of European cranberry (Oxyccocus palustris Pers.) of the Dar Kostromy cultivar and the hybrid form 1-15-635 in vitro at the stage of “proper micropropagation” on the WPM nutrient medium. Clonal micropropagation is most appropriate to use for the rapid production of a large amount of high-quality planting material for forest berry plants. Technologies of clonal micropropagation of European cranberry cultivars of Russian selection need to be improved. Growth-regulating substances are 2-iP cytokinin at the concentrations of 1.0 and 2.0 mg/l and adaptogen Epin-Extra at a concentration of 0.5 ml/l. An increase in the concentration of cytokinin 2-iP from 1.0 to 2.0 mg/l in the WPM nutrient medium contributed to an increase in the number (by 1.2 times) and total length (by 1.3 times) of cranberry microshoots in vitro. The addition of Epin-Extra 0.5 mg/l to the nutrient medium contributed to a increase in the number by 1.1 times and a increase in the total length by 1.2 times of microshoots. Significant differences in the number and length of shoots of European cranberry are not revealed depending on the cultivar or form.
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Gutman, Emmanuel M. "Comment on “Simple thermodynamic derivation of the electrocapillary equations” by L. Makkonen [Surf. Sci. 635 (2015) 61–63]". Surface Science 639 (wrzesień 2015): L5—L8. http://dx.doi.org/10.1016/j.susc.2015.04.011.
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A.K. PATRA, L.M. GARNAYAK, B.B. BEHERA, D. SWAIN i R.K. PAIKARAY. "Productivity, resource use efficiency and economics of rice (Oryza sativa)-based bio-intensive cropping systems in western Odisha". Indian Journal of Agronomy 66, nr 2 (10.10.2001): 157–62. http://dx.doi.org/10.59797/ija.v66i2.2851.
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A field experiment was conducted at Chiplima, Odisha, under irrigated medium-land condition during 201314 to 201617, to evaluate the production potential and economics of 10 rice (Oryza sativa L.)-based bio-intensive cropping systems. Ricepotato (Solanum tubersum L.) + radish [Raphanus raphanistrum subsp. sativus (L.) Domin]pumpkin (Cucurbita pepo L.) + amaranth (Amaranthus viridis L.), ricemaize (Zea mays L.) + coriander (Coriandrum sativum L.)cowpea (Vigna unguiculata L.) + amaranth and ricemaize + radishokra (Abelmoschus esculentus L.) + amaranth cropping systems recorded significantly higher average rice-equivalent yields (REY) (22.72, 20.73 and 19.65 t/ha respectively) than the other rice-based cropping systems, the increase being 143, 122 and 110% over the existing ricegroundnut (Arachis hypogaea) system. Ricetomato (Solanum lycopersicum L.) + amaranthwatermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] + amaranth system had the highest land- use efficiency (97%). Ricepotato + radishpumpkin + amaranth revealed the highest irrigation water-use effi- ciency of 239 kg REY/ha-cm. The highest apparent nutrient-use productivity was realized with the ricemaize + co- riandercowpea + amaranth (46.6 kg REY/kg NPK applied). This system also recorded the highest energy-use ef- ficiency (5.04) and energy productivity (0.41 kg REY/MJ). Ricetomato + fenugreek (Trigonella foenum-graecum L.)cowpea + amaranth (635 man-days) and ricemaize + radishokra + amaranth (625 man-days) created higher employment opportunity. Ricemaize + coriandercowpea + amaranth recorded the highest net returns (`136,544) followed by ricemaize + radishokra + amaranth (`116,803) and ricepotato + radishpumpkin + amaranth (`111,890). These 3 cropping systems also recorded the highest system profitability and crop profitability with higher benefit: cost ratio.
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Sakamoto, Kenichi, Toshihiko Imamura, Kentaro Kihira, Koji Suzuki, Hisashi Ishida, Hiromi Morita, Miyako Kanno i in. "Low Incidence of Osteonecrosis in Childhood Acute Lymphoblastic Leukemia Treated With ALL-97 and ALL-02 Study of Japan Association of Childhood Leukemia Study Group". Journal of Clinical Oncology 36, nr 9 (20.03.2018): 900–907. http://dx.doi.org/10.1200/jco.2017.75.5066.
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Purpose Osteonecrosis (ON) is a serious complication of the treatment of childhood acute lymphoblastic leukemia (ALL); however, data relating to ON in Asian pediatric patients with ALL are scarce. Therefore, we performed a retrospective analysis of cohorts of Japanese patients with ALL to clarify the incidence, clinical characteristics, and risk factors of ON. Patients and Methods The incidence and characteristics of ON were determined in patients with ALL (n = 1,662) enrolled in two studies from the Japan Association of Childhood Leukemia Study (JACLS) group (n = 635 and n = 1,027 patients treated with the ALL-97 and ALL-02 protocols, respectively). Results In total, 24 of 1,662 patients suffered from ON, of which 12 of 635 and 12 of 1,027 patients were treated with the ALL-97 and the ALL-02 protocol, respectively. Of the 24 patients, 23 were older than 10 years. In multivariate analysis, age (≥ 10 years) was the sole significant risk factor for ON ( P < .001). Separate evaluation of patients ≥ 10 years of age indicated a 5-year cumulative incidence of ON of 7.2% (95% CI, 4.0% to 12.6%) and 5.9% (95% CI, 3.3% to 10.4%) in the ALL-97 and the ALL-02 protocol, respectively, which was lower than reported previously, despite an administration of dexamethasone (DEX) similar to that in comparable studies; however, concomitant administration of DEX and l-asparaginase was reduced in the JACLS protocols. Conclusion We identified a low frequency of ON in the JACLS ALL-97 and ALL-02 studies. Although the sole risk factor for ON was age (≥ 10 years), even among high-risk patients, ON incidence was significantly lower than that reported in previous studies. These results suggest that, not only the total amount of DEX, but also how DEX and l-asparaginase are administered, which affects the clearance of DEX, may be associated with ON incidence in patients with ALL.
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Biriș, Iovu-Adrian, Cătălina Oana Barbu i Marius Teodosiu. "Recenzii". Bucovina Forestiera 19, nr 1 (31.07.2019): 95–102. http://dx.doi.org/10.4316/bf.2019.014.
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Nicolae Doniță, Stoica Preda Godeanu, Roxana Corina Sfetea: Population, Species, Biocenosis: An integrating vision. LAP Lambert Academic Publishing. 2019, 109 p. ISBN-13: 978-613-9- 45530-0. Nichiforel L., Managementul forestier cu și fără guvern, Editura Performantica Iași, 2019, 171 p. ISBN 978-606-685-635-5 Fournier R.A., Hall R.J. (eds.), 2017. Hemispherical photography in forest science: theory, methods, applications [Fotografia emisferică în științele silvice: teorie, metode, aplicații] (Vol. 28). Springer. eISSN 978-94-024-1098-3, 318 p Ralf Th., 2017. Data Visualisation with R: 100 Examples [Vizualizarea datelor cu R: 100 de exemple]. Springer. ISBN 978-3-319-49750-1, eISBN 978-3-319-49751-8, 385 p.
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Bessonov, I. S., V. A. Kuznetsov, I. P. Ziryanov, S. S. Sapozhnikov i Yu V. Potolinskaya. "Impact of Diabetes Mellitus and blood glucose levels on the results of treatment of patients with ST-elevation myocardial infarction undergoing percutaneous coronary interventions". Kardiologiia 59, nr 3S (13.04.2019): 16–22. http://dx.doi.org/10.18087/cardio.2520.
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The aim of this study was to evaluate the impact of diabetes mellitus (DM) and glucose levels on the results of treatment of patients with ST-elevation myocardial infarction (STEMI) undergoing percutaneous coronary interventions (PCIs). Materials and methods. Data were collected from all patients (n=1280) with STEMI who were admitted to the coronary care unit and underwent PCIs from 2006 to 2015. 212 (16.6%) patients with DM were compared with 1068 (83.4%) patients without DM (non-DM group). To investigate the influence of the blood glucose levels, all patients were divided into two groups above and below the median of blood glycemia (7.52 mmol/l). Results. Thus, 634 patients with high level of blood glycemia (>7.52 mmol/l) were compared with 635 patients with low level of blood glycemia (≤7.52 mmol/l). In comparing of DM and non-DM groups there were no differences in the rate of death (5.2% vs 4.2%, р=0.526), stent thrombosis (1.4% vs 1.0%, р=0.622), recurrent myocardial infarction (MI) (1.4% vs 1.2%, р=0.813) and major adverse cardiac events (MACE) (7.5% vs 5.4%, р=0.228), which included in-hospital death, recurrent MI and stent thrombosis. The rates of angiographic success (92.9% vs 93.8%, р=0.625) and no-reflow (6.6% vs 5%, р=0.327) also were comparable between groups. The rates of death (6.3% vs 2.5%, р=0.001), MACEs (7.6% vs 4.1%, р=0.008), and no-reflow (6.9% vs 3.6%, р=0,009) were significantly higher in patients with high level of blood glycemia (>7.52 mmol/l). Angiographic success rate (95.1% vs 92.1%, р=0.029) was higher in patients with low level of glycemia (≤7.52 mmol/l). After multivariate adjustment, high level of blood glycemia (>7.52 mmol/l) remained an independent predictor of death (OR=2.28; 95% CI 1.18-4.40, р=0.014), MACE (OR=2.08; 95% CI 1.16-3.75, р=0.014) and no-reflow (OR=2.07; 95% CI 1.15-3.74, р=0.015). At the same time DM wasn’t associated with death, MACE or no-reflow. Conclusion. High level of blood glycemia was an independent predictor of death, MACE and no-reflow in patients with STEMI, undergoing PCI. The presence of DM was not associated with worse in-hospital outcomes.
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Bailey, G. G., i L. L. Needham. "Simultaneous quantification of erythrocyte zinc protoporphyrin and protoporphyrin IX by liquid chromatography." Clinical Chemistry 32, nr 12 (1.12.1986): 2137–42. http://dx.doi.org/10.1093/clinchem/32.12.2137.
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Abstract A simple, rapid, specific, and sensitive isocratic "high-performance" liquid-chromatographic procedure is described for measuring protoporphyrin (PPIX) and zinc protoporphyrin (ZPP) in erythrocytes. A 30-microL whole-blood sample is treated with a solution of formic acid, deproteinized with acetone, and centrifuged. A 20-microL aliquot of the supernate is injected into a system consisting of a stationary phase of mu-Bondapak C18 and a mobile phase of acetone, methanol, water, and formic acid. ZPP and PPIX are detected fluorometrically (lambda ex = 417 nm, lambda em = 635 nm) within 6 min. The range of linearity extends beyond 10 mg/L for ZPP and 580 micrograms/L for PPIX. The detection limits for ZPP and PPIX are 11.9 micrograms/L (6.93 pg) and 2.55 micrograms/L (1.485 pg), respectively. The precision for ZPP and PPIX determinations averaged 2.86 and 5.59%, respectively, for within-day CVs and 4.98 and 8.14, respectively, for among-day CVs. Analytical recoveries averaged 97.2% for ZPP and 101.5% for PPIX. Interferences in the form of fluorescent quenching of ZPP and PPIX by hemin are avoided by chromatographic separation. We also used this method to determine the purity of commercially prepared ZPP, and compared the results obtained with this method with those from an extraction method.
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Ighachane, Mohamed, Zakaria Taki i Mohammed Bouchangour. "An improvement of Alzer-Fonseca-Kovacec’s type inequalities with applications". Filomat 37, nr 22 (2023): 7383–99. http://dx.doi.org/10.2298/fil2322383i.
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In this paper, we start by presenting multiple-term refinement of Young?s and Young?s type inequalities and its reverse using different weights, which extends and unifies two recent and important results due to M. Khosravi (Math. Slovaca 68 (2018), 803-810) and L. Nasiri et al. (Asian-European Journal Math. 15, (2022) No. 07). Further, we mainly present some new real power inequalities of Young?s inequality, extending a key results of Alzer et al. (Linear Multilinear Algebra 63(3) (2015), 622-635), D. Q. Huy et al. (Linear Algebra Appl. 656 (2023), 368-384) and J. Zhao (Bull. Malys. Math. Sci. Soc. 46, No 52 (2023)). As applications of these results, we provide some inequalities for matrices, unitarily invariant norms and traces.
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S.K. RAUTARAY. "Effect of mulching on yield and economics of rainfed rice (Oryza sativa)-based cropping sequences in lower Assam". Indian Journal of Agronomy 50, nr 1 (10.10.2001): 13–15. http://dx.doi.org/10.59797/ija.v50i1.5048.
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A field experiment was conducted during 1999-2000 and 2000-2001 on a silty clay-loam soil, to find out the ef- fect of mulching on yield and economics of winter crops grown after the rice (Oryza sativa L.) and to identify suit- able rice-based cropping sequences under rainfed situations of lower Assam. Rice as base crop gave an yield of 53.1 q/ha with net returns of R s 9,990. Among the sequential crops, radish (Raphanus sativus L.) gave the highest yield (274.2 q/h) followed by potato (Solanum tuberosum L.), tomato (Lycopersicon esculentum Mill. nom. Cons.) and pea (Pisum sativum L.).The interaction of winter crops x mulching showed that the rate of increase in yield ow- ing to mulching was highest (29%) with tomato, followed by potato (21 %). Net returns from potato, tomato and rad- ish were similar (Rs 13, 333 to R s 16, 635/ha) under mulching, while under no mulching net returns from tomato were less than from radish and potato. Yield and net returns were the lowest with gram (Cicer arietinum L.). Rice- potato cropping sequence gave the highest rice-equivalent yield (129.1 q/ha) and production efficiency (57.7 kg/ha/ day) with low land-use efficiency (61.4%). However, net returns (Rs 24,035Iha) under this sequence were similar to that under rice-radish or rice-tomato.
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Pratama, Andhika Satria, Indarto Indarto i Deendarlianto Deendarlianto. "Studi CFD mengenai Pengaruh Sifat Fisis Fluida terhadap Karakteristik Counter-Current Flow Limitation pada Pipa Horizontal". Journal of Mechanical Design and Testing 3, nr 2 (27.12.2021): 118. http://dx.doi.org/10.22146/jmdt.66408.
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Tujuan penelitian ini ialah untuk mempelajari pengaruh densitas cairan, viskositas cairan, dan densitas gas terhadap karakteristik counter current flow limitation atau flooding pada pipa horizontal. Analisis numerik dilakukan menggunakan software CFD Ansys Fluent 2020 R2 student version dengan menerapkan model volume of fluid (VOF). Aparatus penelitian yang digunakan ialah geometri pipa hot leg reaktor PWR tipe German Konvoi skala 1/30 yang terdiri dari reactor pressure vessel, pipa hot leg, dan steam generator. Pipa hot leg yang digunakan memiliki diameter dalam D = 25,4 mm dan panjang pipa horizontal L = 635 mm (L/D = 25). Cairan yang digunakan ialah air, kloroform, larutan gliserin 10%, dan propil asetat, sedangkan gas yang digunakan ialah udara dan uap air. Hasil penelitian menunjukkan bahwa peningkatan densitas cairan menyebabkan peningkatan kecepatan superficial flooding gas, serta menyebabkan pergeseran hydraulic jump dan locus of slugging menjauhi belokan. Hasil dari peningkatan viskositas cairan menunjukkan pola yang berkebalikan dibandingkan hasil dari peningkatan densitas cairan. Penurunan densitas gas menyebabkan flooding terjadi pada kecepatan superficial gas yang lebih tinggi, serta menyebabkan pergeseran hydraulic jump dan locus of slugging menjauhi belokan.
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Nikrawesh, H., AGJ Engbers, S. Völler, SHP Simons, BCP Koch, CAJ Knibbe, IKM Reiss i RB Flint. "P72 Maturation of sildenafil clearance in prematurely born infants with BPD associated pulmonary hypertension". Archives of Disease in Childhood 104, nr 6 (17.05.2019): e47.1-e47. http://dx.doi.org/10.1136/archdischild-2019-esdppp.110.
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BackgroundSildenafil is used as an off-label treatment for bronchopulmonary dysplasia (BPD) associated pulmonary hypertension in prematurely born infants. As there is only limited information on the pharmacokinetics (PK) of sildenafil in this population, the aim of this study is to investigate the PK of sildenafil in prematurely born infants with BPD associated pulmonary hypertension.MethodIn this multicentre study, a population PK model for sildenafil in prematurely born infants was developed based on samples obtained using opportunistic sampling during clinical use of sildenafil. Data of seven subjects (42 plasma samples) were analysed by nonlinear mixed-effect modelling (NONMEM®7.3). Median (range) gestational age (GA) was 26.1 (24.1–27.6) weeks, current bodyweight 1960 (632–3157) grams, birthweight 635 (465–1125) grams and postnatal age (PNA) at start of therapy 64.9 (10.9–89) days. The median (range) treatment duration was 4.9 (1.6–13.1) weeks, with six subjects receiving oral doses of median 2.6 mg/kg/day (1.5–5.3) in three doses and one subject receiving oral and intravenous doses of 6.7 mg/kg/day in two doses.ResultsThe plasma concentration time profiles of sildenafil were best described by a one compartment model. Clearance (CL) and volume of distribution (Vd) for a child with a PNA of 64.9 days and bodyweight of 1.96 kg was 4.42 L/h ((RSE) 11%) and 29.5 L (32%), respectively. PNA was found to significantly influence CL, resulting in an increase of 10.7% in a week, and 43% in a month for a 65-day old infant. No other covariates (i.e. bodyweight, birthweight, GA, postmenstrual age and sex) were identified for CL or Vd.ConclusionIn this PK study, we characterised the pharmacokinetics of sildenafil in prematurely born infants and found that clearance increases with PNA. Due to the limited sample size in this study, further research in a larger population is needed to extend these findings.Disclosure(s)Nothing to disclose
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Edwards, C. A., C. Dieguez i M. F. Scanlon. "Effects of hypothyroidism, tri-iodothyronine and glucocorticoids on growth hormone responses to growth hormone-releasing hormone and His-d-Trp-Ala-Trp-d-Phe-Lys-NH2". Journal of Endocrinology 121, nr 1 (kwiecień 1989): 31–36. http://dx.doi.org/10.1677/joe.0.1210031.
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ABSTRACT The aim of this study was to investigate the role of thyroid hormones and glucocorticoids on GH secretion. Secretion of GH in response to GH-releasing hormone (GHRH) (5 μg/kg) was markedly (P < 0·001) decreased in hypothyroid rats in vivo (peak GH responses to GHRH, 635 ± 88 μg/l in euthyroid rats vs 46 ±15 μg/l in hypothyroid rats). Following treatment with tri-iodothyronine (T3; 20 μg/day s.c. daily for 2 weeks) or cortisol (100 pg/day s.c. for 2 weeks) or T3 plus cortisol, a marked (P <0·01) increase in GH responses to GHRH was observed in hypothyroid rats (peak GH responses, 326 ±29 μg/l after T3 vs 133+19 μg/l after cortisol vs 283 ± 35 μg/l after cortisol plus T3). In contrast, none of these treatments modified GH responses to GHRH in euthyroid animals. Hypothyroidism was also associated with impaired GH responses to the GH secretagogue, Hisd-Trp-Ala-Trp-d-Phe-Lys-NH2 (GHRP-6). Secretion of GH in response to GHRP-6 in vivo was reduced (P <0·01) in hypothyroid rats (peak GH responses, 508 ± 177 μg/l in euthyroid rats vs 203 ± 15 μg/l in hypothyroid rats). In-vitro studies carried out using monolayer cultures of rat anterior pituitary cells derived from euthyroid and hypothyroid rats showed a marked impairment of somatotroph responsiveness to both GHRP-6 and somatostatin in cultures derived from hypothyroid rats. In summary, our data suggest that thyroid hormones and glucocorticoids influence GH secretion by modulating somatotroph responsiveness to different GH secretagogues. Journal of Endocrinology (1989) 121, 31–36
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Rivas, L. J., i K. W. Hinchcliff. "Effect of furosemide and subsequent intravenous fluid administration on right atrial pressure of splenectomized horses". American Journal of Veterinary Research 58, nr 6 (1.06.1997): 632–35. http://dx.doi.org/10.2460/ajvr.1997.58.06.632.
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Abstract Objective To investigate the effect of IV administration of fluids on the furosemide-induced reduction in right atrial pressure (RAP) and relative change in blood volume (BV) of splenectomized mares. Animals 5 splenectomized mares. Procedure RAP was measured by use of a micromanometer placed in the right atrium. Jugular venous blood was collected for measurement of hematocrit, plasma total protein concentration, and hemoglobin concentration. Right atrial pressure was recorded and blood samples were collected immediately before furosemide (1 mg/kg of body weight, IV) administration, then every 15 minutes for 240 minutes. Beginning 120 minutes after furosemide administration, polyionic fluids (lactated Ringer's solution) were administered (2 L q 15 min) for 120 minutes. Results Furosemide induced a significant (P < 0.05) decrease in mean RAP (7.6 ± 1.5 and 3.2 ± 1.2 mm of Hg before and 15 minutes after furosemide administration, respectively), and BV (8.4 ± 1.1 % by 15 minutes). Polyionic fluid administration restored RAP and BV The volume of polyionic fluids administered (32 ± 2 ml/kg) was not significantly different from the volume of urine produced (38 ± 7.8 ml/kg). Difference was not apparent in the relation between change in BV and RAP before or after fluid administration. Conclusions The effect of furosemide on RAP of horses is mediated in large part by furosemide-induced reduction in BV. However, an effect of furosemide on venous compliance cannot be excluded as contributing to the reduction in RAP. (Am J Vet Res 1997;58:632–635)
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ARIFFIN, BADRUL KHALID, i FAZILAH ARIFFIN. "BIODEGRADATION OF AZO DYE (REACTIVE GREEN 19) BY Pseudomonas aeruginosa ISOLATED FROM TEXTILE EFFLUENT". Malaysian Applied Biology 49, nr 4 (19.07.2021): 1–8. http://dx.doi.org/10.55230/mabjournal.v49i4.1560.
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Azo dyes are widely used in the textile industry. The release of these undesirable dye effluents to the environment can be toxic and carcinogenic towards humans and other organisms. The removal of these dyestuff wastes using the biodegradation method is to consider eco-friendlier compared to physical and chemical methods. Thus, this study was conducted to identify and analyze the biodegradation activity of the azo dye by the potential bacteria isolated from textile dye effluent. For decolorization analysis, the isolated bacteria were inoculated into flasks containing mineral salt medium added with Reactive Green 19 dye (50 mg/L) and was incubated for 9 days at 37°C. Spectrophotometry analysis was carried out at 635 nm to analyze the level of azo dye degradation. From the analysis, we found that the optimum pH, temperature, and dye concentration for the decolorization were detected at pH 7, 37°C, and 50 mg/L, respectively. The identified bacteria Pseudomonas aeruginosa showed 94% decolorization of Reactive Green 19 dye after 9 days of incubation. The result of decolorization activity by this bacterial strain can be used in the biological treatment of textile effluent.
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Reshetnyk, K. S., i Yu G. Prysedsky. "Growth, cultural and morphological characteristics of strains of Laetiporus sulphureus (Polyporales, Basidiomycota) under the influence of laser irradiation". Ukrainian Botanical Journal 77, nr 6 (24.12.2020): 472–79. http://dx.doi.org/10.15407/ukrbotj77.06.472.
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The article provides growth, cultural and morphological characteristics of the vegetative mycelium on agar nutrient medium under the influence of laser irradiation for three strains of Laetiporus sulphureus from the Collection of basidiomycete cultures of the Department of Botany and Ecology of Vasyl' Stus Donetsk National University. The study was performed on potatoglucose agar (PGA) at a temperature of 26 ± 1 °C. It has been found that cultural and morphological characteristics of the colonies and the radial rate of their growth depend on the duration of irradiation (5 and 10 s) and the wavelength of light – green (532 nm), blue (405 nm) and red light (635 nm). For all studied strains of L. sulphureus, the most effective irradiation is that with green light (irradiation energy 51.1 mJ/cm2) lasting 10 s. Under the influence of this regime, the rate of radial mycelium growth increased from 23.4% to 66.7%, respectively, and the inoculum and the central zone of the surrounding colony formed a denser and higher mycelium of a pale sandy color. In general, the cultural and morphological features of the mycelial colonies of L. sulphureus strains under different conditions were somewhat different, but they were typical for this species.
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Hein, Sebastian, i Heinrich Spiecker. "Comparative analysis of occluded branch characteristics for Fraxinus excelsior and Acer pseudoplatanus with natural and artificial pruning". Canadian Journal of Forest Research 37, nr 8 (sierpień 2007): 1414–26. http://dx.doi.org/10.1139/x06-308.
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The aim of this study was to develop models on branch characteristics for Fraxinus excelsior L. (common ash) and Acer pseudoplatanus L. (sycamore maple) based on 44 and 25 sample trees, respectively. A total of 635 ash and 334 maple branches were sampled. The data set on artificial pruning was pooled among the two species with a total of 71 branches from 16 trees. The material was used to predict (i) the time for a complete occlusion, (ii) the total radius of the occluded branch inside the trunk, (iii) the branch insertion angle, and (iv) the dead branch portion of the occluded branch. In addition, the effects of species and natural versus artificial pruning were assessed. Generalized hierarchical mixed models with univariate or multivariate approaches were used in this analysis. The diameter of the occluded branch and the stem radial increment played a dominant role as predictors. Artificial pruning led to a significant reduction in occlusion time and a shorter occluded branch radius. Only few species-specific differences were found. Simulations showed a reasonable overall behaviour of the models. The residual variation was tolerable for integrating the models into a growth simulation system.
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Murai, Masayuki, Birendra Bahadur Rana, Itsuro Takamure, Haruki Nakazawa i Mukunda Bhattarai. "Lateness Gene Concerning Photosensitivity Increases Yield, by Applying Low to High Levels of Fertilization, in Rice, a Preliminary Report". Journal of Nepal Agricultural Research Council 6 (17.03.2020): 136–45. http://dx.doi.org/10.3126/jnarc.v6i0.28125.
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Various genes controlling heading time have been reported in rice. An isogenic-line pair of late and early lines “L” and “E” were developed from progenies of the F1 of Suweon 258 × an isogenic line of IR36 carrying Ur1 gene. The lateness gene for photosensitivity that causes the difference between L and E was tentatively designated as “Ex(t)”, although it’s chromosomal location is unknown. The present study was conducted to examine the effects of Ex(t) on yield and related traits in a paddy field in two years. Chemical fertilizers containing N, P2O5 and K2O were applied at the nitrogen levels of 4.00, 9.00 and 18.00 g/m2 in total, being denoted by "N4", "N9" and "N18", respectively, in 2014. L was later in 80%-heading by 18 or 19 days than E. Regarding total brown rice yield (g/m2), L and E were 635 and 577, 606 and 548, and 590 and 501, respectively, at N18, N9 and N4, indicating that Ex(t) increased this trait by 10 to 18%. Ex(t) increased yield of brown rice with thickness above 1.5mm (g/m2), by 9 to 15%. Ex(t) increased spikelet number per panicle by 16 to 22% and spikelet number per m2 by 11 to 18%. Thousand-grain weight (g) was 2 to 4% lower in L than in E. L was not significantly different from E in ripened-grain percentage. Hence, Ex(t) increased yield by increasing spikelet number per panicle. It is suggested that Ex(t) could be utilized to develop high yielding varieties for warmer districts of the temperate zone.
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LI, Chunying, Wei HUANG, M. Brennan HARRIS, Jonathan M. GOOLSBY i Richard C. VENEMA. "Interaction of the endothelial nitric oxide synthase with the CAT-1 arginine transporter enhances NO release by a mechanism not involving arginine transport". Biochemical Journal 386, nr 3 (8.03.2005): 567–74. http://dx.doi.org/10.1042/bj20041005.
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eNOS (endothelial nitric oxide synthase) catalyses the conversion of L-arginine into L-citrulline and NO. Evidence has been presented previously that eNOS is associated with the CAT (cationic amino acid transporter)-1 arginine transporter in endothelial caveolae, and it has been proposed that eNOS–CAT-1 association facilitates the delivery of extracellular L-arginine to eNOS. Definitive proof of a protein–protein interaction between eNOS and CAT-1 is lacking, however, and it is also unknown whether the two proteins interact directly or via an adaptor protein. In the present study, we raised a polyclonal antibody against CAT-1, and show using reciprocal co-immunoprecipitation protocols that eNOS and CAT-1 do indeed form a complex in BAECs (bovine aortic endothelial cells). In vitro binding assays with GST (glutathione S-transferase)–CAT-1 fusion proteins and eNOS show that the two proteins interact directly and that no single CAT-1 intracellular domain is sufficient to mediate the interaction. Overexpression of CAT-1 in BAECs by adenoviral-mediated gene transfer results in significant increases in both L-arginine uptake and NO production by the cells. However, whereas increased L-arginine transport is reversed completely by the CAT-1 inhibitor, L-lysine, increased NO release is unaltered, suggesting that NO production in this in vitro model is independent of CAT-1-mediated transport. Furthermore, eNOS enzymic activity is increased in lysates of CAT-1-overexpressing cells accompanied by increased phosphorylation of eNOS at Ser-1179 and Ser-635, and decreased association of eNOS with caveolin-1. Taken together, these data suggest that direct interaction of eNOS with CAT-1 enhances NO release by a mechanism not involving arginine transport.
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Lowe, C. "Kinematics and critical swimming speed of juvenile scalloped hammerhead sharks". Journal of Experimental Biology 199, nr 12 (1.12.1996): 2605–10. http://dx.doi.org/10.1242/jeb.199.12.2605.
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Kinematics and critical swimming speed (Ucrit) of juvenile scalloped hammerhead sharks Sphyrna lewini were measured in a Brett-type flume (635 l). Kinematic parameters were also measured in sharks swimming in a large pond for comparison with those of sharks swimming in the flume. Sharks in the flume exhibited a mean Ucrit of 65±11 cm s-1 (± s.d.) or 1.17±0.21 body lengths per second (L s-1), which are similar to values for other species of sharks. In both the flume and pond, tailbeat frequency (TBF) and stride length (LS) increased linearly with increases in relative swimming speed (Urel=body lengths traveled per second). In the flume, tailbeat amplitude (TBA) decreased with increasing speed whereas TBA did not change with speed in the pond. Differences in TBF and LS between sharks swimming in the flume and the pond decreased with increases in Urel. Sharks swimming at slow speeds (e.g. 0.55 L s-1) in the pond had LS 19 % longer and TBF 21 % lower than sharks in the flume at the same Urel. This implies that sharks in the flume expended more energy while swimming at comparable velocities. Comparative measurements of swimming kinematics from sharks in the pond can be used to correct for effects of the flume on shark swimming kinematics and energetics.
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Duff, David G. "Taxing Inherited Wealth: A Philosophical Argument". Canadian Journal of Law & Jurisprudence 6, nr 1 (styczeń 1993): 3–62. http://dx.doi.org/10.1017/s0841820900001788.
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Tax reform is, as it should be, a major issue on the political agenda. Wealth taxation should form part of the basis of that agenda if any of the goals, particularly that of equality of opportunity, which we cherish as a civilized and democratic society, are to be realized.Maureen A. Maloney,“Distributive Justice: That is the Wealth Tax Issue,”(1988) 20 Ottawa L. Rev. 601 at 635.After two decades of decline and indifference in the Anglo-American world, the subject of taxing inherited wealth has recently begun to resurface. In the past five years, the number of books and articles on the topic has shown a noticeable increase. And in Ontario, Canada’s most populous and industrialized province, the unexpected election of the New Democratic Party in September 1991, promising to reintroduce a provincial succession duty, has put the issue back on the political agenda.
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Zaitseva, Yu A., A. Ya Bedrov, A. A. Moiseev, Yu P. Kovalchuk, M. I. Kadinskaia, G. I. Popov, G. I. Martynenko, G. V. Rybakov i G. G. Khubulava. "The role of preoperative D-dimer blood level in assessing the risk of open abdominal aortic surgery (statistical study)". Regional blood circulation and microcirculation 23, nr 2 (28.06.2024): 24–29. http://dx.doi.org/10.24884/1682-6655-2024-23-2-24-29.
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Introduction. Changes in the hemostasis and fibrinolysis system in patients with aortoiliac segment lesion can cause the development of specific thromboembolic and hemorrhagic complications, accompanied by a high level of mortality. Preoperative laboratory analysis of the D-dimer blood level may help to objectify the risk assessment of open surgery in this category of patients. Objective. Assessment of the significance of preoperative blood D-dimer level in predicting the development of morbidity and mortality after open surgery on the aortoiliac segment. Materials and methods. The study included 108 patients who were determined the D-dimer blood level before planned reconstructive intervention on the aortoiliac segment at the Pavlov University from 2013 to 2021. The first group consisted of 56 patients with aneurysmal aortoiliac segment lesions, and the second group included 52 patients with occlusive-stenotic lesions of the aortoiliac segment. Results. 92 (85%) patients had an increase of D-dimer level above 500 μg/L, among them 18 patients developed early postoperative complications, and 4 cases were fatal. In 16 (15%) patients with normal D-dimer levels, the postoperative the period was uneventful. Both groups showed statistically significant differences in preoperative blood D-dimer levels depending on the presence or absence of early postoperative complications. In the first group, the median of D-dimer level in the smooth course of the postoperative period was 2035 μg/L, and in patients with complicated course – 3770 μg/L (p=0.04), in the second group these indicators were 635 μg/L and 1231.5 μg/L (p=0.02), respectively. The threshold value of D-dimer level in predicting the risk of death in the first group was 4150 μg/L (p=0.001). Conclusion. The blood D-dimer level may be an additional laboratory risk factor for adverse outcome of open surgery in patients with aortoiliac occlusive disease.
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Park, Young-Jun, i Tomotaro Nishikawa. "Rapid identification of Amaranthus caudatus and Amaranthus hypochondriacus by sequencing and PCR–RFLP analysis of two starch synthase genes". Genome 55, nr 8 (sierpień 2012): 623–28. http://dx.doi.org/10.1139/g2012-050.
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The objective of this study was to develop a PCR–RFLP method to identity the cultivated species of grain amaranth based on variations in the sequences of their starch synthase genes. We sequenced the SSSI and GBSSI loci in 126 accessions of cultivated grain amaranth collected from diverse locations around the world. We aligned the gene sequences and searched for restriction enzyme cleavage sites specific to each species for use in the PCR–RFLP analysis. Our analyses indicated that EcoRI would recognize the sequence 5′-GAATT/C-3′ in the SSSI gene from Amaranthus caudatus L., and TaqI would recognize the sequence 5′-T/CGA-3′ in the GBSSI gene from Amaranthus hypochondriacus L. The PCR products obtained using gene-specific primers were 423 bp (SSSI) and 627 or 635 bp (GBSSI) in length. These products were cut with different restriction enzymes resulting in species-specific RFLP patterns that could be used to distinguish among the cultivated grain amaranths. The results clearly showed that A. caudatus and A. hypochondriacus were easily differentiated at the species level using this method. Therefore, the PCR–RFLP method targeting amaranth starch synthase genes is simple and rapid, and it will be a useful tool for the identification of cultivated species of grain amaranth.
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Garadah, Taysir S., Khalid Bin Thani, Leena Sulibech, Ahmed A. Jaradat, Mohamed E. Al Alawi i Haytham Amin. "Risk Stratification and in Hospital Morality in Patients Presenting with Acute Coronary Syndrome (ACS) in Bahrain". Open Cardiovascular Medicine Journal 12, nr 1 (21.02.2018): 7–17. http://dx.doi.org/10.2174/1874192401812010007.
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Background: Risk factors and short-term mortality in patients presented with Acute Coronary Syndrome (ACS) in Bahrain has not been evaluated before. Aim: In this prospective observational study, we aim to determine the clinical risk profiles of patients with ACS in Bahrain and describe the incidence, pattern of presentation and predictors of in-hospital clinical outcomes after admission. Methods: Patients with ACS were prospectively enrolled over a 12 month period. The rate of incidence of risk factors in patients was compared with 635 non-cardiac patient admissions that matched for age and gender. Multiple logistic regression analysis was used to predict poor outcomes in patients with ACS. The variables were ages >65 years, body mass index (BMI) >28 kg/m2, GRACE (Global Registry of Acute Coronary Events) score >170, history of diabetes mellitus (DM), systolic hypertension >180 mmHg, level of creatinine >160 μmol/l and Heart Rate (HR) on admission >90 bpm, serum troponin rise and ST segment elevation on the ECG. Results: Patients with ACS (n=635) were enrolled consecutively. Mean age was 61.3 ± 13.2 years, with 417 (65.6%) male. Mean age for patients with ST-segment elevation myocardial infarction (STEMI, n=156) compared with non-STEMI (NSTEMI, n=158) and unstable angina (UA, n=321) was 56.5± 12.8 vs 62.5±14.0 years respectively. In-hospital mortality was 5.1%, 3.1% and 2.5% for patients with STEMI, NSTEMI, and UA, respectively. In STEMI patients, thrombolytic therapy was performed in 88 (56.5%) patients and 68 (43.5%) had primary coronary angioplasty (PCI). The predictive value of different clinical variables for in-hospital mortality and cardiac events in the study were: 2.8 for GRACE score >170, 3.1 for DM, 2.2 for SBP >180 mmHg, 1.4 for age >65 years, 1.8 for BMI >28, 1.7 for creatinine >160 μmol/L, 2.1 for HR >90 bpm, 2.2 for positive serum troponin and 2.3 for ST elevation. Conclusion: Patients with STEMI compared with NSTEMI and UA were of younger age. There was higher in-hospital mortality in STEMI compared with NSTEMI and UA patients. The most significant predictors of death or cardiac events on admission in ACS were DM, GRACE Score >170, systolic hypertension >180 mmHg, positive serum troponin and HR >90 bpm.
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Crosby, S. R., C. D. Anderton, M. Westwood, J. M. P. Holly, S. C. Cwyfan Hughes, M. Gibson, C. A. Morrison, R. J. Young i A. White. "Measurement of insulin-like growth factor-II in human plasma using a specific monoclonal antibody-based two-site immunoradiometric assay". Journal of Endocrinology 137, nr 1 (kwiecień 1993): 141–50. http://dx.doi.org/10.1677/joe.0.1370141.
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ABSTRACT An immunoradiometric assay (IRMA) for the measurement of insulin-like growth factor-II (IGF-II) in human plasma has been developed, optimized and evaluated clinically in normal subjects and patients with disorders of the GH/IGF-I axis. Six monoclonal antibodies (MAbs) to recombinant human IGF-II (rhIGF-II) were produced, all of which had low cross-reactivity with rhIGF-I (< 0·01%) and insulin (< 0·01%). Compatibility of pairs of MAbs was tested in two-site IRMAs using three radioiodinated MAbs and three MAbs linked to Sephacryl S-300 (with separation of bound and free radiolabelled MAb by sucrose layering). Seven pairs of MAbs bound rhIGF-II and the combination of 125I-labelled W3D9 and W2H1 linked to solid phase was selected. The optimized assay had a completion time of 4 h, a minimum detection limit of 30 ng/ml (2·5 standard deviations from the zero standard) and detected a single peak of endogenous IGF-II in normal plasma which co-eluted with rhIGF-II after acid gel chromatography. IGF-II was measured in formic acid/acetone extracts of plasma from 16 normal subjects (mean 685, range 516–1008 μg/l), four acromegalic patients (mean 637, range 553–700 μg/l), fourteen patients with type-1 diabetes (mean 635, range 247–753 μg/l), nine patients with uraemia (mean 423, range 78–850 μg/l), and three patients with Laron-type GH insensitivity (75, 35 and 36 μg/l). No significant fluctuations were detected between samples obtained hourly from 08.00 to 19.00 h in normal subjects. Low levels of IGF-binding proteins (IGFBPs) remaining in plasma extracts may interfere with the measurement of IGF-II and give rise to falsely elevated IGF-II levels in radioimmunoassays or falsely suppressed levels in IRMAs. Such interference did not occur with the IRMA when used to measure IGF-II in extracts from normal subjects, acromegalic patients and patients with type-1 diabetes, and the addition of excess rhIGF-I in order to displace IGF-II from residual IGFBPs had no effect on IGF-II measurements in these samples. However, levels of IGF-II measured in extracts from patients with Laron-type GH insensitivity and patients with uraemia increased markedly after preincubation with excess rhIGF-I. The accurate measurement of IGF-II by IRMA in extracts from these subjects therefore requires the displacement of IGF-II from IGFBPs prior to assay. We conclude that, in contrast to radioimmunoassays, the two-site IRMA developed here provides a practical, rapid and specific method for the measurement of IGF-II in human plasma. Journal of Endocrinology (1993) 137, 141–150
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Emmanouilidis, Nikos, Jörg A. Müller, Mark D. Jäger, Stephan Kaaden, Fabian A. Helfritz, Zeynep Güner, Holger Kespohl i in. "Surgery and radioablation therapy combined: introducing a 1-week-condensed procedure bonding total thyroidectomy and radioablation therapy with recombinant human TSH". European Journal of Endocrinology 161, nr 5 (listopad 2009): 763–69. http://dx.doi.org/10.1530/eje-08-0641.
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ObjectiveThe objective of this study was to determine whether the use of recombinant human TSH (rhTSH) to stimulate radioiodine uptake after thyroidectomy is as efficacious as a period of withholding thyroid hormones, while at the same time avoiding hypothyroidism, reducing sick leave time and shortening the hospital stay.DesignOur aim was to compare the standard procedure of differentiated thyroid cancer treatment, which consists of thyroidectomy followed by 4 weeks of hypothyroidism and a conclusive ablative activity of 131iodine, with a new shortened treatment in which l-thyroxine (T4) medication is initiated a day after thyroidectomy, followed by application of rhTSH stimulation and subsequent ablation a few days after surgery. We presumed our treatment to represent the most sophisticated strategy for the reduction in sick leave days overall without any reduction in safety or the efficacy of ablative therapy.MethodsPatients (n=25) were randomized either for surgery and rhTSH stimulation or surgery and l-T4 abstinence before the first application of radioiodine. Ablation success was determined by neck ultrasound and serum thyroglobulin during follow-up. RhTSH receivers were monitored for an average of 635 days (s.d.±289) and patients in l-T4 abstinence for an average of 624 days (s.d.±205). Both groups were statistically compared for significant differences in treatment efficacy, safety and overall time of sick leave.Results and conclusionsOur shortened treatment proved to be equally efficacious and safe in comparison with the conventional therapy regimen. At the same time, it showed economic advantages through the reduction in average sick leave time from ∼29 days (l-T4 abstinence) down to ∼6 days (rhTSH stimulation) as well as sustaining the patient's quality of life by the complete avoidance of hypothyroidism.
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Geng, Tao, Mark T. Morgan i Arun K. Bhunia. "Detection of Low Levels of Listeria monocytogenes Cells by Using a Fiber-Optic Immunosensor". Applied and Environmental Microbiology 70, nr 10 (październik 2004): 6138–46. http://dx.doi.org/10.1128/aem.70.10.6138-6146.2004.
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ABSTRACT Biosensor technology has a great potential to meet the need for sensitive and nearly real-time microbial detection from foods. An antibody-based fiber-optic biosensor to detect low levels of Listeria monocytogenes cells following an enrichment step was developed. The principle of the sensor is a sandwich immunoassay where a rabbit polyclonal antibody was first immobilized on polystyrene fiber waveguides through a biotin-streptavidin reaction to capture Listeria cells on the fiber. Capture of cells on the fibers was confirmed by scanning electron microscopy. A cyanine 5-labeled murine monoclonal antibody, C11E9, was used to generate a specific fluorescent signal, which was acquired by launching a 635-nm laser light from an Analyte 2000 and collected by a photodetector at 670 to 710 nm. This immunosensor was specific for L. monocytogenes and showed a significantly higher signal strength than for other Listeria species or other microorganisms, including Escherichia coli, Enterococcus faecalis, Salmonella enterica, Lactobacillus plantarum, Carnobacterium gallinarum, Hafnia alvei, Corynebacterium glutamicum, Enterobacter aerogenes, Pseudomonas aeruginosa, and Serratia marcescens, in pure or in mixed-culture setup. Fiber-optic results could be obtained within 2.5 h of sampling. The sensitivity threshold was about 4.3 × 103 CFU/ml for a pure culture of L. monocytogenes grown at 37°C. When L. monocytogenes was mixed with lactic acid bacteria or grown at 10°C with 3.5% NaCl, the detection threshold was 4.1 × 104 or 2.8 × 107 CFU/ml, respectively. In less than 24 h, this method could detect L. monocytogenes in hot dog or bologna naturally contaminated or artificially inoculated with 10 to 1,000 CFU/g after enrichment in buffered Listeria enrichment broth.
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Munguti, J., H. Charo-Karisa, MA Opiyo, EO Ogello, E. Marijani, L. Nzayisenga i D. Liti. "Nutritive value and availability of commonly used feed ingredients for farmed nile tilapia (Oreochromis niloticus L.) and African catfish (Clarias gariepinus, Burchell) in Kenya, Rwanda and Tanzania". African Journal of Food, Agriculture, Nutrition and Development 12, nr 51 (28.05.2012): 6135–55. http://dx.doi.org/10.18697/ajfand.51.11085.
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Commonly utilized feed ingredients for culture of Nile tilapia ( Oreochromis niloticus ) and Africa n catfish ( Clarias gariepinus ) from Kenya , Tanzania and Rwanda were collected over a period of six months (January - June 2010) and evaluated for their nutritive composition through proximate analysis. Most of the fish feed ingredients were found in all the three countries except a few that were unique to one or two countries as detailed in the results . Feed ingredients of animal origin exhibited significantly (P <0.05) higher crude protein levels (407 - 635 g/kg DM ) compared to the feed ingredients of plant based origin . Freshwater shrimp s ( Caridina nilotic a ) contained the highest crude protein levels of 635 g/kg DM. Feed ingredients of animal origin had low ether extracts (EE) with an exception of Omena fish ( Rastrineobola argentea ) . Crude fibre (CF) was generally higher on feed ingredients of plant origin and ranged between 55 - 368 g/kg DM while Nitrogen Free Extract s (Nf E) and ash content were higher in the feedstuff s of plant origin with an exception of maize bran which had the lowest value. Omena fish and freshwater shrimp had higher ash content of 182 and 228 g/kg DM compared to other feed ingredients of animal origin, which may indicate contamination with soil particles during drying process . Based on the feed ingredient availability, potential competition with other human uses, content of crude protein and crude fibre and the feasibility of removal of anti -nutritional factors, C . nilotica , blood meal, meat and bone meal , were identified as most promising sources of animal based protein. Cotton (Gossypium spp) and sunflower ( Helianthus annuus ) seed cakes provided the best option as source of processed plant based protein. L eaves of gallant soldier ( Galiso nga parviflora ) , sweet potato ( Ipomoea batatus ), cassava ( Manihot esculenta ) and papaya ( Papaya carica ) were identified as high potential feedstuffs of plant origin either processed or in raw form for small -scale fish farming . We recommend that in well fertilized semi- intensive ponds , maize ( Zea mays ) , wheat ( Triticum aestivum ) and rice ( Oryza sativa ) bran may be utilized where available to supplement natural pond food
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Sipilä, Laura, Heli Ruotsalainen, Raija Sormunen, Naomi L. Baker, Shireen R. Lamandé, Miia Vapola, Chunguang Wang, Yoshikazu Sado, Attila Aszodi i Raili Myllylä. "Secretion and Assembly of Type IV and VI Collagens Depend on Glycosylation of Hydroxylysines". Journal of Biological Chemistry 282, nr 46 (14.09.2007): 33381–88. http://dx.doi.org/10.1074/jbc.m704198200.
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Most lysines in type IV and VI collagens are hydroxylated and glycosylated, but the functions of these unique galactosylhydroxylysyl and glucosylgalactosylhydroxylysyl residues are poorly understood. The formation of glycosylated hydroxylysines is catalyzed by multifunctional lysyl hydroxylase 3 (LH3) in vivo, and we have used LH3-manipulated mice and cells as models to study the function of these carbohydrates. These hydroxylysine-linked carbohydrates were shown recently to be indispensable for the formation of basement membranes (Ruotsalainen, H., Sipilä, L., Vapola, M., Sormunen, R., Salo, A. M., Uitto, L., Mercer, D. K., Robins, S. P., Risteli, M., Aszodi, A., Fässler, R., and Myllylä, R. (2006) J. Cell Sci. 119, 625–635). Analysis of LH3 knock-out embryos and cells in this work indicated that loss of glycosylated hydroxylysines prevents the intracellular tetramerization of type VI collagen and leads to impaired secretion of type IV and VI collagens. Mice lacking the LH activity of LH3 produced slightly underglycosylated type IV and VI collagens with abnormal distribution. The altered distribution and aggregation of type VI collagen led to similar ultrastructural alterations in muscle to those detected in collagen VI knockout and some Ullrich congenital muscular dystrophy patients. Our results provide new information about the function of hydroxylysine-linked carbohydrates of collagens, indicating that they play an important role in the secretion, assembly, and distribution of highly glycosylated collagen types.
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Arancibia-Bravo, María P., Alejandro López-Valdivieso, Luís F. Flores i Luís A. Cisternas. "Effects of Potassium Propyl Xanthate Collector and Sodium Sulfite Depressant on the Floatability of Chalcopyrite in Seawater and KCl Solutions". Minerals 10, nr 11 (9.11.2020): 991. http://dx.doi.org/10.3390/min10110991.
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This study demonstrates the effects of a potassium propyl xanthate (PPX) collector and sodium sulfite (Na2SO3) depressant on pure chalcopyrite in synthetic seawater (SSW) and potassium chloride (KCl) solutions. SSW solutions with 35 g/L of salt and 0.01-M KCl were used for microflotation and zeta potential tests. Particles sized between 200# and 100# (75–150 µm) were used, and the pH was between 8.0 and 8.5. The surface of the mineral and its interaction with the collector were characterized using Raman spectrometry. The zeta potential of the chalcopyrite was measured in KCl solution at a pH range of 3–12 using the collector and depressant at a monodispersed particle size of 635# (20 µm). The results indicate that the floatability of chalcopyrite is not affected by the presence of PPX collectors in SSW solutions. SSW provides better recoveries than KCl solutions with values of 91.42% and 88.15%, respectively. The Na2SO3 depressant does not hinder the mineral floatability throughout the entire concentration range used; however, special care must be taken when adjusting the pH range to avoid increasing the zeta potential.
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Saha Ray, S., B. P. Poddar i R. K. Bera. "Analytical Solution of a Dynamic System Containing Fractional Derivative of Order One-Half by Adomian Decomposition Method". Journal of Applied Mechanics 72, nr 2 (1.03.2005): 290–95. http://dx.doi.org/10.1115/1.1839184.
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The fractional derivative has been occurring in many physical problems, such as frequency-dependent damping behavior of materials, motion of a large thin plate in a Newtonian fluid, creep and relaxation functions for viscoelastic materials, the PIλDμ controller for the control of dynamical systems, etc. Phenomena in electromagnetics, acoustics, viscoelasticity, electrochemistry, and materials science are also described by differential equations of fractional order. The solution of the differential equation containing a fractional derivative is much involved. Instead of an application of the existing methods, an attempt has been made in the present analysis to obtain the solution of an equation in a dynamic system whose damping behavior is described by a fractional derivative of order 1/2 by the relatively new Adomian decomposition method. The results obtained by this method are then graphically represented and compared with those available in the work of Suarez and Shokooh [Suarez, L. E., and Shokooh, A., 1997, “An Eigenvector Expansion Method for the Solution of Motion Containing Fraction Derivatives,” ASME J. Appl. Mech., 64, pp. 629–635]. A good agreement of the results is observed.
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Paine, Thomas E., i Mark A. Bennett. "SEED TREATMENT EFFECTS ON VOLATILE ALDEHYDE PRODUCTION IN GERMINATING sh2 SWEET CORN." HortScience 28, nr 5 (maj 1993): 529d—529. http://dx.doi.org/10.21273/hortsci.28.5.529d.
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Seeds of two shrunken-2 (sh2) sweet corn (Zea mays L.) cultivars, `Crisp'n Sweet 710' and `Camelot', were used to relate volatile production to seed quality and seedling establishment. The five seed treatments evaluated were a hydration and drying cycle, a biological control of Pythium, a hydration and drying cycle followed by a biological control, a fungicide treatment, and an untreated check. For the aldehyde assays, 50 seeds were germinated in a flask for 24 h with a test tube of a 3-methyl-2-benzo-thiazolinone hydrazone (MBTH) solution. One ml of the solution was then reacted with FeCl3, diluted with acetone, and absorbance read at 635 nm. A field study was run under cool soil conditions (10-16°C) for seedling emergence and growth. Aldehyde production from the seeds did not correlate with field results for seed treatments, but did show a distinct difference between cultivars. The biological control with the hydration and drying cycle significantly reduced aldehyde production. This may contribute to the effectiveness of biological treatments since Pythium spores can be stimulated by volatile compounds released from germinating seeds.
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Ritter, A., C. M. Regalado i G. Aschan. "Fog Water Collection in a Subtropical Elfin Laurel Forest of the Garajonay National Park (Canary Islands): A Combined Approach Using Artificial Fog Catchers and a Physically Based Impaction Model". Journal of Hydrometeorology 9, nr 5 (1.10.2008): 920–35. http://dx.doi.org/10.1175/2008jhm992.1.
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Abstract Fog precipitation has long been assumed as an additional water source in the relic laurel ecosystems of the Canary Islands, located at 500–1400 m MSL. However, to what extent fog water can contribute to the laurel forest water balance is not yet clear. Combining data from artificial fog catchers and a physically based impaction model, the authors evaluated the potential contribution of fog water captured by needle-leafed Erica arborea L. trees in a selected watershed of the Garajonay National Park (La Gomera Island) for a 2-yr period (February 2003–January 2005). Fog water collection was measured with artificial catchers at four micrometeorological stations placed at 1145, 1185, 1230, and 1270 m MSL. Average fog water collection was only significant at the highest measurement site (one order of magnitude greater than at lower altitudes), totaling 496 L m−2 yr−1 during the 2-yr period. The average fog water yield in the first and second annual periods ranged between 0.2–5.0 and 0.1–2.1 L m−2 day−1, respectively. Rainfall exhibited seasonality, distinguishing between rainy and dry seasons, while fog water collection was distributed more evenly throughout the year. Regarding fog water captured by the vegetation, the impaction model predicted a significant amount of fog water potentially collected by a single E. arborea tree, on the order of 1810–2090 L yr−1. Taking tree population density into account, the yearly average water contribution to the soil surface by wind-driven fogs was 251–281 mm, whereas annual rainfall was 635 and 1088 mm, respectively. The hourly course of micrometeorological variables shows a 58% reduction in global radiation under foggy conditions and a concomitant 3°–6°C mean temperature decrease compared to fog-free periods. Thus, limiting evapotranspiration may also be a relevant effect of fog in this subtropical elfin cloud forest.
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Wright, Trevor. "Pharmaceuticals: classes, therapeutic agents, areas of application. John L. McGuire (ed.). Wiley -VCH, Weinheim, 2001. vi?+?2315 pages. �635. ISBN 3-527-29874-6". Applied Organometallic Chemistry 15, nr 9 (2001): 773. http://dx.doi.org/10.1002/aoc.215.
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Girard, I., J. L. Aalhus, J. A. Basarab, I. L. Larsen i H. L. Bruce. "Modification of muscle inherent properties through age at slaughter, growth promotants and breed crosses". Canadian Journal of Animal Science 91, nr 4 (grudzień 2011): 635–48. http://dx.doi.org/10.4141/cjas2011-058.
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Girard, I., Aalhus, J. L., Basarab, J. A., Larsen, I. L. and Bruce, H. L. 2011. Modification of muscle inherent properties through age at slaughter, growth promotants and breed crosses. Can. J. Anim. Sci. 91: 635–648. A 24 factorial experiment tested the interactions of slaughter age (12–13 or 18–20 mo), growth hormone use, β-adrenergic agonist (β-AA) use and breed cross [Hereford–Aberdeen Angus (HAA) or Charolais–Red Angus (CRA)] on the composition, fibre types, and connective tissue characteristics of m. semitendinosus (ST) and m. gluteus medius (GM) from 112 crossbred steers. Muscle weights increased with slaughter age, implantation and CRA genetics (P<0.05), but were not affected by ractopamine hydrochloride (RAC) (P>0.10).Animal age increased fast glycolytic (FG) and decreased fast oxidative glycolytic (FOG) fibre percentages by 7.2 and 6.6%, respectively, in the ST and increased slow oxidative (SO) and FOG fibre areas in both muscles (P<0.05). Cross-sectional areas of all fibre types were increased in the ST with implantation. In the GM, implantation increased SO (3.1%) and reduced FOG (3.2%) fibre percentages, while RAC reduced the SO (3.8%) and increased the FG (6.1%) fibre percentages (P<0.05).Only GM total collagen content increased with slaughter age (P<0.05),but collagen solubility decreased with slaughter age for both muscles (P<0.05). CRA genetics increased FG percentage in the GM of yearling-fed steers and increased moisture and protein and reduced fat contents of both muscles (P<0.05). In the muscles studied, IMP, slaughter age and animal genetics induced greater changes in muscle inherent properties than RAC.
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van Meeteren, Uulke, i Annie van Gelder. "The Role of Leaves in Photocontrol of Flower Bud Abscission in Hibiscus rosa-sinensis L. `Nairobi'". Journal of the American Society for Horticultural Science 125, nr 1 (styczeń 2000): 31–35. http://dx.doi.org/10.21273/jashs.125.1.31.
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When compared with exposure to darkness, exposing Hibiscus rosa-sinensis L. `Nairobi' plants to red light (635 to 685 nm, 2.9 μmol·m-2·s-1) delayed flower bud abscission, while exposure to far-red light (705 to 755 nm, 1.7 μmol·m-2·s-1) accelerated this process. Flower bud abscission in response to light quality appears to be controlled partly by the presence of leaves. The delay of bud abscission was positively correlated to the number of leaves being exposed to red light. Excluding the flower buds from exposure to red or far-red light, while exposing the remaining parts of the plants to these light conditions, did not influence the effects of the light exposure on bud abscission. Exposing only the buds to red light by the use of red light-emitting diodes (0.8 μmol·m-2·s-1) did not prevent dark-induced flower bud abscission. Exposing the whole plants, darkness or far-red light could only induce flower bud abscission when leaves were present; bud abscission was totally absent when all leaves were removed. To prevent flower bud abscission, leaves had to be removed before, or at the start of, the far-red light treatment. These results suggest that in darkness or far-red light, a flower bud abscission-promoting signal from the leaves may be involved.
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Cazzola, Mario, Gaetano Bergamaschi, Laura Tonon, Eloisa Arbustini, Maurizia Grasso, Elena Vercesi, Giovanni Barosi, Paolo Emilio Bianchi, Gaetano Cairo i Paolo Arosio. "Hereditary Hyperferritinemia-Cataract Syndrome: Relationship Between Phenotypes and Specific Mutations in the Iron-Responsive Element of Ferritin Light-Chain mRNA". Blood 90, nr 2 (15.07.1997): 814–21. http://dx.doi.org/10.1182/blood.v90.2.814.
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Abstract Recent reports have described families in whom a combination of elevated serum ferritin not related to iron overload and congenital nuclear cataract is transmitted as an autosomal dominant trait. We have studied the molecular pathogenesis of hyperferritinemia in two families showing different phenotypic expression of this new genetic disorder. Serum ferritin levels ranged from 950 to 1,890 μg/L in affected individuals from family 1, and from 366 to 635 μg/L in those from family 2. Cataract was clinically manifested in family 1 and asymptomatic in family 2. By using monoclonal antibodies specific for the H and L ferritin subunits, serum ferritin was found to be essentially L type in both normal and affected individuals. The latter also showed normal amounts of H-type ferritin in circulating mononuclear cells; on the contrary, L-type ferritin contents were 13 times normal in family 1 and five times normal in family 2 on average. Serum ferritin was glycosylated in both normal and affected individuals. There was a close relationship between mononuclear cell L-type ferritin content and serum ferritin concentration (r = 0.95, P < .00001), suggesting that the excess production of ferritin in cells was directly responsible for the hyperferritinemia. The dysregulated L-subunit synthesis was found to result from different point mutations in a noncoding sequence of genomic L-subunit DNA, which behaves as an mRNA cis-acting element known as iron regulatory element (IRE). Affected individuals from family 1 were heterozygous for a point mutation (a single G to A change) in the highly conserved, three-nucleotide motif forming the IRE bulge. Affected members from family 2 were heterozygous for a double point mutation in the IRE lower stem. Using a gel retardation assay, the observed molecular lesions were shown to variably reduce the IRE affinity for an iron regulatory protein (IRP), which inhibits ferritin mRNA translation. The direct relationship between the degree of hyperferritinemia and severity of cataract suggests that this latter is the consequence of excessive ferritin production within the lens fibers. These findings provide strong evidence that serum ferritin is a byproduct of intracellular ferritin synthesis and that the L-subunit gene on chromosome 19 is the source of glycosylated serum ferritin. From a practical standpoint, this new genetic disorder should be taken into account by clinicians when facing a high serum ferritin in an apparently healthy person.
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Cazzola, Mario, Gaetano Bergamaschi, Laura Tonon, Eloisa Arbustini, Maurizia Grasso, Elena Vercesi, Giovanni Barosi, Paolo Emilio Bianchi, Gaetano Cairo i Paolo Arosio. "Hereditary Hyperferritinemia-Cataract Syndrome: Relationship Between Phenotypes and Specific Mutations in the Iron-Responsive Element of Ferritin Light-Chain mRNA". Blood 90, nr 2 (15.07.1997): 814–21. http://dx.doi.org/10.1182/blood.v90.2.814.814_814_821.
Pełny tekst źródłaStreszczenie:
Recent reports have described families in whom a combination of elevated serum ferritin not related to iron overload and congenital nuclear cataract is transmitted as an autosomal dominant trait. We have studied the molecular pathogenesis of hyperferritinemia in two families showing different phenotypic expression of this new genetic disorder. Serum ferritin levels ranged from 950 to 1,890 μg/L in affected individuals from family 1, and from 366 to 635 μg/L in those from family 2. Cataract was clinically manifested in family 1 and asymptomatic in family 2. By using monoclonal antibodies specific for the H and L ferritin subunits, serum ferritin was found to be essentially L type in both normal and affected individuals. The latter also showed normal amounts of H-type ferritin in circulating mononuclear cells; on the contrary, L-type ferritin contents were 13 times normal in family 1 and five times normal in family 2 on average. Serum ferritin was glycosylated in both normal and affected individuals. There was a close relationship between mononuclear cell L-type ferritin content and serum ferritin concentration (r = 0.95, P < .00001), suggesting that the excess production of ferritin in cells was directly responsible for the hyperferritinemia. The dysregulated L-subunit synthesis was found to result from different point mutations in a noncoding sequence of genomic L-subunit DNA, which behaves as an mRNA cis-acting element known as iron regulatory element (IRE). Affected individuals from family 1 were heterozygous for a point mutation (a single G to A change) in the highly conserved, three-nucleotide motif forming the IRE bulge. Affected members from family 2 were heterozygous for a double point mutation in the IRE lower stem. Using a gel retardation assay, the observed molecular lesions were shown to variably reduce the IRE affinity for an iron regulatory protein (IRP), which inhibits ferritin mRNA translation. The direct relationship between the degree of hyperferritinemia and severity of cataract suggests that this latter is the consequence of excessive ferritin production within the lens fibers. These findings provide strong evidence that serum ferritin is a byproduct of intracellular ferritin synthesis and that the L-subunit gene on chromosome 19 is the source of glycosylated serum ferritin. From a practical standpoint, this new genetic disorder should be taken into account by clinicians when facing a high serum ferritin in an apparently healthy person.
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Bosedasgupta, Somdeb, Benu Brata Das, Souvik Sengupta, Agneyo Ganguly, Amit Roy, Gayatri Tripathi i Hemanta K. Majumder. "Amino acids 39–456 of the large subunit and 210–262 of the small subunit constitute the minimal functionally interacting fragments of the unusual heterodimeric topoisomerase IB of Leishmania". Biochemical Journal 409, nr 2 (21.12.2007): 481–89. http://dx.doi.org/10.1042/bj20071358.
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The unusual, heterodimeric topoisomerase IB of Leishmania shows functional activity upon reconstitution of the DNA-binding large subunit (LdTOPIL; or L) and the catalytic small subunit (LdTOPIS; or S). In the present study, we generated N- and C-terminal-truncated deletion constructs of either subunit and identified proteins LdTOPIL39–456 (lacking amino acids 1–39 and 457–635) and LdTOPIS210–262 (lacking amino acids 1–210) as the minimal interacting fragments. The interacting region of LdTOPIL lies between residues 40–99 and 435–456, while for LdTOPIS it lies between residues 210–215 and 245–262. The heterodimerization between the two fragments is weak and therefore co-purified fragments showed reduced DNA binding, cleavage and relaxation properties compared with the wild-type enzyme. The minimal fragments could complement their respective wild-type subunits inside parasites when the respective subunits were down-regulated by transfection with conditional antisense constructs. Site-directed mutagenesis studies identify Lys455 of LdTOPIL and Asp261 of LdTOPIS as two residues involved in subunit interaction. Taken together, the present study provides crucial insights into the mechanistic details for understanding the unusual structure and inter-subunit co-operativity of this heterodimeric enzyme.