Rozprawy doktorskie na temat „Ionic imaging by mass spectrometry”
Utwórz poprawne odniesienie w stylach APA, MLA, Chicago, Harvard i wielu innych
Sprawdź 50 najlepszych rozpraw doktorskich naukowych na temat „Ionic imaging by mass spectrometry”.
Przycisk „Dodaj do bibliografii” jest dostępny obok każdej pracy w bibliografii. Użyj go – a my automatycznie utworzymy odniesienie bibliograficzne do wybranej pracy w stylu cytowania, którego potrzebujesz: APA, MLA, Harvard, Chicago, Vancouver itp.
Możesz również pobrać pełny tekst publikacji naukowej w formacie „.pdf” i przeczytać adnotację do pracy online, jeśli odpowiednie parametry są dostępne w metadanych.
Przeglądaj rozprawy doktorskie z różnych dziedzin i twórz odpowiednie bibliografie.
Rowland, Tyson G. "Accurate ionic bond energy measurements with TCID mass spectrometry and imaging PEPICO spectroscopy". Scholarly Commons, 2012. https://scholarlycommons.pacific.edu/uop_etds/809.
Pełny tekst źródłaYuen, Wei Hao. "Ion imaging mass spectrometry". Thesis, University of Oxford, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.564395.
Pełny tekst źródłaGoodwin, Lee. "Capillary electrophoresis-mass spectrometry and tandem mass spectrometry studies of ionic agrochemicals". Thesis, University of York, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.398906.
Pełny tekst źródłaCobice, Diego Federico. "Mass spectrometry imaging of steroids". Thesis, University of Edinburgh, 2015. http://hdl.handle.net/1842/21032.
Pełny tekst źródłaPalmer, Andrew D. "Information processing for mass spectrometry imaging". Thesis, University of Birmingham, 2014. http://etheses.bham.ac.uk//id/eprint/5472/.
Pełny tekst źródłaStryffeler, Rachel Bennett. "New analytical approaches for mass spectrometry imaging". Diss., Georgia Institute of Technology, 2015. http://hdl.handle.net/1853/54892.
Pełny tekst źródłaJung, Seokwon. "Surface characterization of biomass by imaging mass spectrometry". Diss., Georgia Institute of Technology, 2012. http://hdl.handle.net/1853/45906.
Pełny tekst źródłaHenderson, Fiona. "Mass spectrometry imaging of lipid profiles in disease". Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/mass-spectrometry-imaging-of-lipid-profiles-in-disease(f1b202b1-2a6e-416e-ab81-321ef4f0e24d).html.
Pełny tekst źródłaGuo, Ang. "Improving the performance of microscope mass spectrometry imaging". Thesis, University of Oxford, 2018. http://ora.ox.ac.uk/objects/uuid:aa94a7f6-00ee-4b56-ba65-f6946799d5f2.
Pełny tekst źródłaNakata, Yoshihiko. "Imaging Mass Spectrometry with MeV Heavy Ion Beams". 京都大学 (Kyoto University), 2009. http://hdl.handle.net/2433/124537.
Pełny tekst źródłaHulme, Heather E. "Mass spectrometry imaging to investigate host-microbe interactions". Thesis, University of Glasgow, 2018. http://theses.gla.ac.uk/8930/.
Pełny tekst źródłaFu, Tingting. "3D and High Sensitivity Micrometric Mass Spectrometry Imaging". Thesis, Université Paris-Saclay (ComUE), 2017. http://www.theses.fr/2017SACLS218/document.
Pełny tekst źródłaMass spectrometry imaging has been shown of great interest in addressing biological questions by providing simultaneously chemical and spatial information. Particularly, TOF-SIMS is well recognized for its high spatial resolution (< 1 µm) which is essential in disclosing chemical information within a submicron area. The increasing use of TOF-SIMS in characterizing biological samples has greatly benefited from the introduction of new cluster ion sources. However, the ionization/desorption of the analytes under impacts of large clusters is still poorly understood. On the other hand, technically, current commercial TOF-SIMS instruments generally cannot provide sufficient mass resolution or mass accuracy for molecular identification, making analyses of complex biological systems especially challenging when no MS/MS fragmentation is available. Thus this thesis is aimed to get a better understanding of ion production under cluster impacts, to explore the MS/MS capability of the parallel imaging MS/MS Spectrometer (PHI nanoTOF II), as well as to apply TOF-SIMS to map important wood metabolites with high spatial resolution.In order to understand ion production under impacts of massive argon clusters, internal energy distributions of secondary ions were measured using survival yield method which involves the analyses of a series of benzylpyridinium ions. Investigation of various impacting conditions (energy, velocity, cluster size) suggested that velocity of the clusters play a major role in internal energy distribution and molecular fragmentation in the low energy per atom regime (E/n < 10 eV). The MS/MS fragmentation and parallel imaging capabilities of the newly designed PHI nanoTOF II spectrometer were evaluated by in situ MS/MS mapping of bioactive metabolites rubrynolide and rubrenolide in Amazonia wood species Sextonia rubra. Then this parallel imaging MS/MS technique was applied to perform in situ identification of related precursor metabolites in the same tree species. 2D and 3D TOF-SIMS imaging were carried out to target the plant cells that biosynthesize rubrynolide and rubrenolide. The results led to the proposal of a possible biosynthesis pathway of these two metabolites. In addition, to expand the application of TOF-SIMS imaging in wood chemistry analysis, radial distribution of wood extractives in the heartwood of European larch was also investigated
DONG, YONGHUI. "Mass spectrometry imaging: looking fruits at molecular level". Doctoral thesis, country:IT, 2014. http://hdl.handle.net/10449/24270.
Pełny tekst źródłaDong, Yonghui. "Mass Spectrometry Imaging: Looking Fruits at Molecular Level". Doctoral thesis, Università degli studi di Trento, 2014. https://hdl.handle.net/11572/368984.
Pełny tekst źródłaDong, Yonghui. "Mass Spectrometry Imaging: Looking Fruits at Molecular Level". Doctoral thesis, University of Trento, 2014. http://eprints-phd.biblio.unitn.it/1286/1/Thesis_Yonghui_Dong.pdf.
Pełny tekst źródłaFornai, L. "Molecular Imaging of the heart by mass spectrometry". Doctoral thesis, Università degli studi di Padova, 2011. http://hdl.handle.net/11577/3421675.
Pełny tekst źródłaIntroduzione Le malattie cardiovascolari rappresentano nel mondo la prima causa di morte, contando 17.1 milioni di morti ogni anno. Attualmente i meccanismi fisiopatologici alla base delle patologie sono in larga parte ancora sconosciuti. Capire la natura dei complessi processi biologici in atto sia nel miocardio cardiaco sano che malato richiede l’identificazione e la localizzazione degli stessi elementi molecolari coinvolti. METODO Utilizzando tecniche complementari di spettrometria di massa d’immagine (SMI) quali la spettrometria di massa a ioni secondari (Secondary Ion Mass Spectrometry, SIMS) e la spettrometria di massa a desorbimento /ionizzazione laser assistita da matrice (Matrix-assisted laser desorption/ionization, MALDI) abbiamo analizzato le principali componenti del cuore del ratto: il pericardio, il miocardio, l’endocardio, le valvole e i grandi vasi al fine di studiare ed identificare l’originale distribuzione di atomi, lipidi, peptici e proteine nel tessuto cardiaco normale. Quaranta sezioni di tessuto cardiaco sono state acquisite e ricostruite ottenendo un database tridimensionale. RISULTATI L’analisi della superficie delle sezione di tessuto cardiaco ha generato molteplici ioni secondari con un intervallo di massa che raggiunge i 1500 m/z. Utilizzando la modalita’ negativa abbiamo identificato il colesterolo e gli ioni relativi ad esso che mostrato un alta intensita’ in entrambi gli atri, l’aorta, l’arteria polmonare e pericardio. La colina corrispondente a 105 m/z di massa molecolare risulta essere localizzata in entrambi gli atri, aorta, arteria polmonare, valvole atrioventricolari e valvole semilunari ma non risulta essere presente sulla superficie ventricolare. Sono state identificate molecole appartenenti al diacilglicerolo come acido Oleico, Linoleico [OL]+ corrispondenti alla massa molecolare di 602 m/z e [OO]+ (Oleico,Oleico) con massa molecolare di 604 m/z. Le immagini ottenute dalla ricostruzione tridimensionale mostrano una specifica localizzazione complementare tra il sodio, il potassio e gli ioni con massa molecolare di 145 m/z e 667 m/z. Il sodio e’maggiormente localizzato nelle regioni cardiache corrispondenti agli atri, mentre il potassio e’ maggiormente localizzato nelle regioni corrispondenti alla superficie ventricolari. Lo ione con massa molecolare di 667 m/z e’ localizzato con molta precisione all’interno della parete dell’aorta e lo ione con massa molecolare di 145 m/z e’ localizzato a livello delle regioni atriali. CONCLUSIONI Al fine di promuovere un’ulteriore ricerca in patologia cardiovascolare, riportiamo l’identificazione delle caratteristiche molecole che mappano l’organizzazione spaziale delle strutture cardiache del cuore del ratto. Una serie di immagini ottenute da sezioni successive del cuore potrebbero inizialmente essere utilizzate come un atlante molecolare e similmente, ad un atlante basato sulle immagini ottiche, essere ampiamente utilizzato come referente sia dal punto di vista fisiologico che anatomico. L’aiuto apportato da questo progetto e’ l’ottimizzazione dei dati ottenuti dall’analisi SIMS e lo sviluppo della tecnica per la ricostruzione tridimensionale al fine di investigare e visualizzare le differenti molecole localizzate nelle strutture del cuore di ratto. I risultati qui riportati rappresentano la prima ricostruzione tridimensionale ottenuta con immagini SIMS, del cuore di ratto.
Arribard, Yann. "Analyse de matière extraterrestre primitive par imagerie hyperspectrale infrarouge et spectrométrie de masse TOF-SIMS". Electronic Thesis or Diss., université Paris-Saclay, 2023. http://www.theses.fr/2023UPASP005.
Pełny tekst źródłaSo-called primitive extraterrestrial matter is characterized by its low chemical evolution since its formation. It is found in particular as one of the constituents of the fragments of small bodies of the Solar system, such as asteroids. The study of samples from these bodies can thus make it possible to better understand its origin and its evolution.In this thesis, my work focused on the analysis of primitive matter and more particularly on the study of carbonaceous chondrites having undergone aqueous alteration. The first part of my thesis focuses on the analysis of mineral and organic phases within petrological type 2 CM chondrites using infrared and Raman spectroscopy techniques as well as time-of-flight secondary ionization mass spectrometry. (TOF-SIMS). These techniques benefit from a good complementarity in the characterization of the different phases that interest us. They are also coupled with imagery, which makes it possible to study the link that may exist between the different mineral and organic phases. I used a new unsupervised process for analyzing infrared hyperspectral data, which made it possible to determine spectral parameters characterizing the state of progress of the aqueous alteration of the samples, in particular of their mineral phase, while relating to their chemical evolution. Raman spectroscopy made it possible to highlight differences in the structure of the polyaromatic organic matter within the different samples. Finally, the TOF-SIMS also highlighted a difference in the structure of the organic matter while confirming and clarifying the differences in co-localization between organic matter and mineral phase observed by hyperspectral imaging between the samples.The second part of my thesis focused on the study of the effectiveness of a new linear accelerator - Andromeda (IJCLab) - as a primary source for TOF-SIMS on analogues of primitive chondrite matter. I produced these organic analogues in the laboratory to simulate insoluble organic matter, the majority of organic matter in chondrites. I checked the characteristics of these analogues by infrared spectroscopy, X-ray spectroscopy and TOF-SIMS. They remain different from CM organic matter in terms of poly-aromatic structure, but similar in terms of elemental composition and insoluble character. I have produced mineral analogues from earth rocks similar to minerals found in CM chondrite. The measurements that I carried out on these analogues and on chondrites show both the potential and the current limits of TOF-SIMS coupled to Andromede, and suggest areas for improvement with a view to increasing, in particular, the masse resolution
Zabet, Moghaddam Masoud. "MALDI mass spectrometry and ionic liquids applications in functional protein analysis /". [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=982074360.
Pełny tekst źródłaRace, Alan M. "Investigation and interpretation of large mass spectrometry imaging datasets". Thesis, University of Birmingham, 2016. http://etheses.bham.ac.uk//id/eprint/6718/.
Pełny tekst źródłaHalford, Edward. "Microscope-mode imaging mass spectrometry with the PImMS camera". Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:bf2d51d4-5037-4be4-8e3b-29861f2ae005.
Pełny tekst źródłaSun, Xiaobo. "Forensic Applications of Gas Chromatography/Mass Spectrometry, High Performance Liquid Chromatography--Mass Spectrometry and Desorption Electrospray Ionization Mass Spectrometry with Chemometric Analysis". Ohio University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1329517616.
Pełny tekst źródłaLum, Tsz Shan. "Elemental imaging and speciation for bioanalysis". HKBU Institutional Repository, 2016. https://repository.hkbu.edu.hk/etd_oa/328.
Pełny tekst źródłaGray, Andrew Peter. "Exploring gas-phase ionic liquid aggregates by mass spectrometry and computational chemistry". Thesis, University of Edinburgh, 2012. http://hdl.handle.net/1842/5861.
Pełny tekst źródłaHerman, Stephanie. "Automatic detection of protein degradation markers in mass spectrometry imaging". Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-275072.
Pełny tekst źródłaHamilton, Jason S. "Disease Tissue Imaging and Single Cell Analysis with Mass Spectrometry". Thesis, University of North Texas, 2017. https://digital.library.unt.edu/ark:/67531/metadc984137/.
Pełny tekst źródłaDhunna, Manan. "Desorption Electrospray Ionization Mass Spectrometry Imaging: Instrumentation, Optimization and Capabilities". BYU ScholarsArchive, 2014. https://scholarsarchive.byu.edu/etd/3969.
Pełny tekst źródłaAtkinson, Sally Jayne. "Fundamental aspects of imaging matrix assisted laser desorption/ionisation mass spectrometry". Thesis, Sheffield Hallam University, 2008. http://shura.shu.ac.uk/19293/.
Pełny tekst źródłaChen, Hongwen. "Investigations of ionic and neutral species in the gas phase by tandem mass spectrometry". Thesis, University of Ottawa (Canada), 1992. http://hdl.handle.net/10393/7647.
Pełny tekst źródłaTang, Ho-wai, i 鄧浩維. "Studies on surface-assisted laser desorption/ionization and its analytical application in imaging mass spectrometry". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B47145559.
Pełny tekst źródłapublished_or_final_version
Chemistry
Doctoral
Doctor of Philosophy
Balluff, Benjamin. "MALDI imaging mass spectrometry in clinical proteomics research of gastric cancer tissues". Diss., lmu, 2013. http://nbn-resolving.de/urn:nbn:de:bvb:19-155986.
Pełny tekst źródłaWinter, Benjamin. "Novel methods in imaging mass spectrometry and ion time-of-flight detection". Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:43db5039-0490-4f97-8519-4d3ed4e30ca3.
Pełny tekst źródłaDjidja, Marie-Claude. "Examination of tumour tissues by direct MALDI-mass spectrometry imaging and profiling". Thesis, Sheffield Hallam University, 2009. http://shura.shu.ac.uk/20662/.
Pełny tekst źródłaEndres, Kevin J. "Mass Spectrometry Methods For Macromolecules: Polymer Architectures, Cross-Linking, and Surface Imaging". University of Akron / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=akron1553096604194835.
Pełny tekst źródłaVelarde, Luis Antonio. "Photoinitiated Dynamics of Cluster Anions via Photoelectron Imaging and Photofragment Mass Spectrometry". Diss., The University of Arizona, 2008. http://hdl.handle.net/10150/195042.
Pełny tekst źródłaDENTI, VANNA. "Development of multi-omic mass spectrometry imaging approaches to assist clinical investigations". Doctoral thesis, Università degli Studi di Milano-Bicocca, 2022. http://hdl.handle.net/10281/365169.
Pełny tekst źródłaThe field of spatial omics defines the gathering of different techniques that allow the detection of significant alterations of biomolecules in the context of their native tissue or cellular structures. As such, they extend the landscape of biological changes occurring in complex and heterogeneous pathological tissues, such as cancer. However, additional molecular levels, such as lipids and glycans, must be studied to define a more comprehensive molecular snapshot of disease and fully understand the complexity and dynamics beyond pathological condition. Among the spatial-omics techniques, matrix-assisted laser desorption/ionisation (MALDI)-mass spectrometry imaging (MSI) offers a powerful insight into the chemical biology of pathological tissues in a multiplexed approach where several hundreds of biomolecules can be examined within a single experiment. Thus, MALDI-MSI has been readily employed for spatial omics studies of proteins, peptides and N-Glycans on clinical formalin-fixed paraffin-embedded (FFPE) tissue samples. Conversely, MALDI-MSI analysis of lipids has always been considered not feasible on FFPE samples due to the loss of a great amount of lipid content during washing steps with organic solvents, with the remaining solvent-resistant lipids being involved in the formalin cross-links. In this three-year thesis work, novel MALDI-MSI approaches for spatial multi-omics analysis on clinical FFPE tissue samples were developed. The first three publications reported in this thesis focused on the development of protocols for MALDI-MSI of lipids in FFPE samples. In particular, two of them describe a sample preparation method for the detection of positively charged phospholipids ions, mainly phosphatidylcholines (PCs), in clinical clear cell Renal Cell Carcinoma (ccRCC) samples and in a xenograft model of breast cancer. The third publication reports the possibility to use negatively charged phospholipids ions, mainly phosphatidylinositols (PIs), to define lipid signatures able to distinguish colorectal cancers with different amount of tumour infiltrating lymphocytes (TILs). The final work proposes a unique multi-omic MALDI-MSI method for the sequential analysis of lipids, N-Glycans and tryptic peptides on a single FFPE section. Specifically, the method feasibility was first established on murine brain technical replicates. The method was consequently used on ccRCC samples, as a proof of concept, assessing a more comprehensive characterisation of the tumour tissue when combining the multi-level molecular information. Altogether, these findings pave the way for new MSI-based spatial multi-omics approach aiming at an extensive and more precise molecular portrait of disease.
Zheng, Jun. "Supercritical Fluid Chromatography of Ionic Compounds". Diss., Virginia Tech, 2005. http://hdl.handle.net/10919/29519.
Pełny tekst źródłaPh. D.
Asogan, Dhinesh. "A non-contact laser ablation cell for mass spectrometry". Thesis, Loughborough University, 2011. https://dspace.lboro.ac.uk/2134/11014.
Pełny tekst źródłaXu, Yang. "Multimodal Spectral Microscopy and Imaging Mass Spectrometry of Biomolecules in Cells and Tissues". University of Toledo / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1333769758.
Pełny tekst źródłaMason, Kyle. "Stepwise Solvation of Organic Radical Cations by Ionic Hydrogen and Halogen Bonding in the Gas Phase". VCU Scholars Compass, 2019. https://scholarscompass.vcu.edu/etd/6024.
Pełny tekst źródłaOlivero, Daniel. "Traumatic brain injury biomarker discovery using mass spectrometry imaging of 3D neural cultures". Thesis, Georgia Institute of Technology, 2011. http://hdl.handle.net/1853/41102.
Pełny tekst źródłaAboulmagd, Khodier Sarah. "Analysis of Lipids in Kidney Tissue Using High Resolution MALDI Mass Spectrometry Imaging". Doctoral thesis, Humboldt-Universität zu Berlin, 2018. http://dx.doi.org/10.18452/19443.
Pełny tekst źródłaMass spectrometry imaging is indispensable for studying the spatial distribution of molecules within a diverse range of biological samples. Since its introduction, MALDI has become a dominant imaging method, which proved useful to sort out the complexity of lipid structures in biological tissues. The role of cisplatin in the treatment of human malignancies is well-established. However, nephrotoxicity is a limiting side effect that involves an acute injury of the proximal tubule and alterations in the renal lipid profile. This evolved the motivation to study the spatial distribution of lipids in the kidney tissue of cisplatin-treated rats to shed light on the lipid signaling pathways involved. A method for mapping of lipid distributions in kidney sections using MALDI-LTQ-Orbitrap was developed, utilizing the high performance of orbitrap detection. The distribution of kidney lipids in cisplatin-treated samples revealed clear differences with respect to control group, which could be correlated to the proximal tubule injury. The findings highlight the usefulness of MALDI MSI as complementary tool for clinical diagnostics. Furthermore, assessment of the ion images of lipids in cisplatin-treated kidney mostly considered qualitative aspects. Relative quantitative comparisons were limited by the variable influence of experimental and instrumental conditions. Hence, the necessity developed to establish a normalization method allowing comparison of lipid intensity in MALDI imaging measurements of different samples. The method employed an inkjet printer to apply a mixture of the MALDI matrix and dual lipid-metal internal standards. Using ICP-MS, the metal internal standard allowed to confirm the consistency of the matrix and internal standards application. Applying the method to normalize ion intensities of kidney lipids demonstrated excellent image correction and successfully enabled relative quantitative comparison of lipid images in control and cisplatin-treated samples.
PIGA, ISABELLA. "Proteomics tools and mass spectrometry imaging techniques for the molecular characterization of pancreas". Doctoral thesis, Università di Siena, 2016. http://hdl.handle.net/11365/1007351.
Pełny tekst źródłaVillacob, Raul A. "Development of a Primary Ion Column for Mass Spectrometry-Based Surface Analysis". FIU Digital Commons, 2016. http://digitalcommons.fiu.edu/etd/2561.
Pełny tekst źródłaBerrueta, Razo Irma. "Molecular imaging of mouse brain tissue using Cluster Time-of-Flight Secondary Ion Mass Spectrometry". Thesis, University of Manchester, 2015. https://www.research.manchester.ac.uk/portal/en/theses/molecular-imaging-of-mouse-brain-tissue-using-cluster-timeofflight-secondary-ion-mass-spectrometry(a350dc50-5337-4d32-a95c-24c617bbba97).html.
Pełny tekst źródłaSui, Ping. "Molecular Signatures of Neuropathic Pain : Revealing Pain-Related Signaling Processes in Spinal Cord Using Mass Spectrometric Methodologies". Doctoral thesis, Uppsala universitet, Analytisk kemi, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-251334.
Pełny tekst źródłaLiu, Qiang. "Fundamental Study and Method Development for Surface-based Laser Desorption Ionization Imaging Mass Spectrometry". NCSU, 2009. http://www.lib.ncsu.edu/theses/available/etd-02262009-143514/.
Pełny tekst źródłaRodrigues, Lívia Riberti 1988. "Análise de impurezas de formas farmacêuticas sólidas por MALDI Mass Spectrometry Imaging (MALDI-MSI)". [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/312437.
Pełny tekst źródłaTexto em português e inglês
Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas
Made available in DSpace on 2018-08-25T05:33:49Z (GMT). No. of bitstreams: 1 Rodrigues_LiviaRiberti_M.pdf: 1203619 bytes, checksum: a17baf81fa013532bd6c9d451b2336f2 (MD5) Previous issue date: 2014
Resumo: Atualmente, as doenças cardiovasculares constituem uma das primeiras causas de mortes no Brasil e no mundo. Neste cenário, as estatinas constituem uma notável classe de medicamentos redutores de colesterol e têm sido associadas com uma expressiva diminuição da morbidade e mortalidade cardiovascular para pacientes em prevenção primária ou secundária da doença coronariana. Elas agem inibindo competitivamente a enzima HMG-CoA redutase, através da afinidade destes fármacos pelo sítio ativo da enzima. Esta enzima é responsável por catalisar a conversão do substrato HMG-CoA em mevalonato, um dos precursores do colesterol. A crescente necessidade e busca por medicamentos cada vez mais efetivos traz a preocupação na segurança destes produtos para seus usuários. Neste sentido, o conhecimento das impurezas e produtos de degradação torna-se necessário para garantir sua qualidade. Uma técnica muito utilizada para análises de impurezas e degradantes é a espectrometria de massas, pois é uma técnica sensível e seletiva e permite elucidar as estruturas químicas presentes na formulação do medicamento. Sendo assim, amostras de Atorvastatina cálcica foram analisadas pela técnica de espectrometria de massas por imagem (MALDI-MSI), permitindo a quantificação de impurezas do medicamento através da imagem da distribuição dessa impureza no comprimido. Dessa forma, é possível minimizar o preparo de amostra e obter um melhor conhecimento da formulação
Abstract: Currently, cardiovascular diseases constitute one of the first causes of deaths in Brazil and in the world. In this scenario, the statins are a notable class of medicines and cholesterol reducers have been associated with a significant reduction in cardiovascular morbidity and mortality for patients in primary or secondary prevention of coronary heart disease. They act by inhibiting competitively the enzyme HMG-CoA reductase, through the affinity of these drugs by the active site of the enzyme. This enzyme is responsible for catalyzing the conversion of HMG-CoA to mevalonate substrate, one of the precursors of cholesterol. The growing need and search for increasingly effective drugs brings the concern on the safety of these drugs for their users. In this sense, the knowledge of the impurities and degradation products becomes necessary to ensure their quality. A widely used technique for analysis of impurities and degrading is mass spectrometry, because it is a sensitive and selective technique and allows elucidating the chemical structures of the present formulation of the medicinal product. Thus, samples of Atorvastatin calcium were analyzed by the technique of mass spectrometry imaging (MALDI-MSI), which allows the quantification of impurities from the medicine through the image of the distribution of impurity in the tablet. That way, it is possible minimize sample preparation and get a better understanding of the formulation
Mestrado
Ciencias Biomedicas
Mestra em Ciências Médicas
Gorishek, Emma Lee. "Laser Ablation Inductively Coupled Plasma Mass Spectrometry and Raman Spectroscopy Imaging of Biological Tissues". Thesis, University of North Texas, 2016. https://digital.library.unt.edu/ark:/67531/metadc849725/.
Pełny tekst źródłaGarrett, Timothy J. "Imaging small molecules in tissue by matrix-assisted laser desorption/ionization tandem mass spectrometry". [Gainesville, Fla.] : University of Florida, 2006. http://purl.fcla.edu/fcla/etd/UFE0013807.
Pełny tekst źródłaDooley, Patrick W. Corkum Paul B. "Molecular imaging using femtosecond laser pulses". *McMaster only, 2003.
Znajdź pełny tekst źródła