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O'Hagan, Derek Thomas. "Pharmaceutical formulations as immunological adjuvants". Thesis, University of Nottingham, 1987. http://eprints.nottingham.ac.uk/13211/.
Pełny tekst źródłaAlmeida, Antonio J. L. "Particulate carriers as immunological adjuvants". Thesis, Aston University, 1993. http://publications.aston.ac.uk/12609/.
Pełny tekst źródłaWhite, Karen Louise, i n/a. "Modified liposomes as adjuvants". University of Otago. School of Pharmacy, 2005. http://adt.otago.ac.nz./public/adt-NZDU20070126.131417.
Pełny tekst źródłaSvelander, Lena. "Studies on immunological mechanisms of induced arthritis in the rat /". Stockholm, 2002. http://diss.kib.ki.se/2002/91-7349-349-X/.
Pełny tekst źródłaFong, Long-yan, i 方朗茵. "Immunomodulatory properties of probiotic bacteria". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/208173.
Pełny tekst źródłapublished_or_final_version
Biological Sciences
Doctoral
Doctor of Philosophy
Gursel, Mayda. "Use of liposomes and cytokines as immunological adjuvants in vaccines". Thesis, University College London (University of London), 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338640.
Pełny tekst źródłaChan, Wing-keung. "The immunomodulatory effects of purified b-glucans and b-glucan containing herbs". Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/HKUTO/record/B39557996.
Pełny tekst źródłaChan, Wing-keung. "The immunomodulatory effects of purified [beta]-glucans and [beta]-glucan containing herbs /". View the Table of Contents & Abstract, 2007. http://sunzi.lib.hku.hk/hkuto/record/B38724674.
Pełny tekst źródłaCheung, Ka-wai, i 張嘉瑋. "The immunomodulatory effect of Brazilian green propolis and its uniquecompound Artepillin C". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45900231.
Pełny tekst źródłaRoßmann, Laura [Verfasser]. "In-depth immunological analysis of prototypic adjuvants for vaccine formulations / Laura Roßmann". Mainz : Universitätsbibliothek der Johannes Gutenberg-Universität Mainz, 2020. http://d-nb.info/1223379299/34.
Pełny tekst źródłaLee, Cheuk-lun. "Immunomodulatory properties of polysaccharopeptide derived from Coriolus versicolor and its combined effect with Cyclosporine a in activated human T-cells". Click to view the E-thesis via HKUTO, 2005. http://sunzi.lib.hku.hk/hkuto/record/B36199497.
Pełny tekst źródłaLee, Cheuk-lun, i 李卓倫. "Immunomodulatory properties of polysaccharopeptide derived from Coriolus versicolor and its combined effect with Cyclosporine a inactivated human T-cells". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2005. http://hub.hku.hk/bib/B36199497.
Pełny tekst źródłaChan, Wing-keung, i 陳永強. "The immunomodulatory effects of purified {221}-glucans and {221}-glucan containing herbs". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B39557996.
Pełny tekst źródłaHeath, Andrew. "Assessment of anti- CD40 monoclonal antibodies as novel immunological adjuvants in conjugate vaccines". Thesis, University of Sheffield, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.500260.
Pełny tekst źródłaPrasad, Vijay Narayana Sharma Bhagawati. "Assessment of Anti-CD40 Monoclonal Antibodies as Novel Immunological Adjuvants in Conjuate Vaccines". Thesis, University of Sheffield, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.500680.
Pełny tekst źródłaLima, Fernanda Aparecida de. "Estudo das propriedades adjuvantes da vacina celular pertussis em combinação com o antígeno PspA de Streptococcus pneumoniae". Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-19092012-164854/.
Pełny tekst źródłaStreptococcus pneumoniae (pneumococcus) is a pathogen that causes respiratory infections leading to the death of 1 million children per year, worldwide. Here, the adjuvant property of the whole cell pertussis vaccine (wP) in combination with the PspA5 antigen from S. pneumoniae was evaluated in animal models. Combinations of PspA5 with wP, wPlow (whole cell pertussis vaccine with low amount of LPS) or with the DTPlow vaccine were inoculated in mice by nasal or subcutaneous routes. The protective response induced by nasal immunization with PspA5-wP and PspA5-wPlow against the lethal challenge with pneumococci was characterized by increased systemic and mucosal anti-PspA5 antibodies and influx of TCD4+, TCD8+ and B cells in the lungs of mice. The subcutaneous inoculation of PspA5-DTPlow also protected against the lethal challenge with pneumococci and the response was characterized by increased levels of systemic and mucosal anti-PspA5 IgG. There was no prejudice to the responses against diphtheria, tetanus or pertussis when PspA5 was combined with DTPlow.
Nader, Oriana Mayorga. "Análise de diferentes adjuvantes associados ao peptídeo P10 usados no tratamento de camundongos BALB/c infectados com Paracoccidioides brasiliensis". Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-18062013-105357/.
Pełny tekst źródłaParacoccidioidomycosis (PCM) is a systemic granulomatous disease caused by the dimorphic fungus Paracoccidioides brasiliensis. The gp43 is the major diagnostic antigen that contains the peptide P10. This peptide has 15 amino acids and develops a Th1- dependent IFN-g immune response. In this work, we compare the effectiveness of different adjuvants in BALB/c mice infected intratracheally with virulent isolate Pb18. Alum, Complete Adjuvant and Incomplete Freund\'s adjuvant, fliC flagellin from Salmonella enterica and cationic lipid were tested in combination or not with P10. After 52 days of infection, the cationic lipid showed the best results with the least number of colony forming units, reduction of fibrosis, low number of fungal cells in lung tissue and the significant increase of IFN-g and TNF-a with reduced levels of IL-4 and IL-10. These results suggest that, the interaction of peptide P10 with cationic lipids can lead to a better immune response mediated by Th1 type cells by avoiding the rapid spread of experimental PCM.
Santos, Fernanda Lucio dos. "Avaliação da qualidade e potencial atividade adjuvante do lipídio A de Bordetella pertussis". Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-14092011-164106/.
Pełny tekst źródłaCurrently a number of substances are being evaluated for possible adjuvant activity, with high power but also high toxicity, which prevents them from being introduced into clinical routine, such as lipopolysaccharide (LPS), cell wall component of Gram negative bacteria. The aim of this study was to characterize a byproduct of Bordetella pertussis LPS (BpLipídioA), through the development and standardization of quality control tests in order to later use this sub-product as an adjuvant vaccine. The knowledge about the product during all phases of research and development led to the selection of optimal physico-chemical, biological and microbiological tests as well as its acceptable range and detection limits. The adjuvant activity of this product was performed with the recombinant hepatitis B vaccine, which showed promissory results. Using this product in emulsion or suspension forms had to be possible to reduce the amount of antigen in the vaccine dose, which increases the installed production capacity of vaccines.
Garcia-Garbi, Natalio. "Interaction of immunostimulants and stress on innate defence mechanisms of rainbow trout, Oncorhynchus mykiss". Thesis, University of Stirling, 1998. http://hdl.handle.net/1893/26672.
Pełny tekst źródłaDurairajan, Siva Sundara Kumar. "Biological screening and isolation of immunomodulatory compounds from endophytic fungi from Tripterygium wilfordii". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B31245274.
Pełny tekst źródłaFu, Hoi-man Kelvin. "The immunomodulatory effects of Chinese medicinal products Yun Zhi and Danshen : flow cytometric studies /". Click to view the E-thesis via HKUTO, 2000. http://sunzi.lib.hku.hk/hkuto/record/B42575916.
Pełny tekst źródłaShankar, Sucharita P. "Glycoprotein-mediated interactions of dendritic cells with surfaces of defined chemistries". Diss., Atlanta, Ga. : Georgia Institute of Technology, 2007. http://hdl.handle.net/1853/29790.
Pełny tekst źródłaCommittee Chair: Julia Babensee ; Committee Members: Barbara Boyan, John Brash, Andres Garcia, and Niren Murthy. Part of the SMARTech Electronic Thesis and Dissertation Collection.
Connell, David James. "Silica nanoparticles as adjuvants for novel vaccines : a combined immunological, simulation and physical characterisation study". Thesis, University of Strathclyde, 2016. http://digitool.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=27908.
Pełny tekst źródłaGomes, Priscila Aparecida Dal Pozo. "Desenvolvimento de novas abordagens vacinais contra a Síndrome Hemolítica Urêmica (SHU) baseadas em variantes atóxicos da toxina Stx2 de Eschirichia coli enterohemorrágica (EHEC)". Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-19032014-104714/.
Pełny tekst źródłaShiga toxin (Stx)-producing Escherichia coli strains (STEC) cause the Hemolytic Uremic Syndrome (HUS), a severe illness. The Stx is an AB5 toxin and comprises a single catalytic A subunit, endowed with protein synthesis inhibitory effect, and five B subunits, involved in the binding to glycolipid receptors on the surface of target cells. The protein Stx2DAB was administered to mice combined with different adjuvants: a heat-labile toxin (LT), derived from enterotoxigenic E. coli (ETEC) strains, the flagellin FliCi of S. Typhimurium, alum or Freund\'s adjuvant. Additionally mice were immunized with denatured toxin. The results showed that sera from mice immunized with recombinant Stx2DAB plus Freund adjuvant displayed the highest anti-Stx2 antibody titers with maximum protection of 77% to a lethal challenge with the Stx2 holotoxin, however animals immunized with denatured toxin showed no protection, despite high serum titers achieved. The results show the potential protector of vaccine antigen as a vaccine subunit and the importance of conformation in the protection.
Lee, Kin-wah Terence, i 李建華. "FTY720, a novel pharmaceutical therapy for hepatocellular carcinoma". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B29797858.
Pełny tekst źródłaParker, Todd Avery. "Elucidating the immunoactivity of a goat serum peptide". Diss., Mississippi State : Mississippi State University, 2002. http://library.msstate.edu/etd/show.asp?etd=etd-03072002-141009.
Pełny tekst źródłaQuaid, Padraic Joseph. "Synthesis of novel chemical adjuvants for the modulation and study of CD1-d mediated immunological processes". Thesis, University of Birmingham, 2017. http://etheses.bham.ac.uk//id/eprint/7861/.
Pełny tekst źródłaSverdrup, Berit. "Aspects of the role of mineral oil as immunological adjuvant in rheumatoid arthritis /". Stockholm, 2007. http://diss.kib.ki.se/2007/978-91-7357-112-8/.
Pełny tekst źródłaAbdalla, Amir Osman. "Immunological and clinical long-term effects of idiotype vaccination in multiple myeloma patients /". Stockholm, 2007. http://diss.kib.ki.se/2007/978-91-7357-114-2/.
Pełny tekst źródłaLendemans, Dirk G., i n/a. "Novel cationic preparations of iscoms as vaccine carriers". University of Otago. School of Pharmacy, 2006. http://adt.otago.ac.nz./public/adt-NZDU20060810.141916.
Pełny tekst źródła傅凱文 i Hoi-man Kelvin Fu. "The immunomodulatory effects of Chinese medicinal products Yun Zhi andDanshen: flow cytometric studies". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2000. http://hub.hku.hk/bib/B42575916.
Pełny tekst źródłaTeixeira, Aline Florencio. "Avaliação terapêutica da formulação vacinal baseada no peptídeo P10 derivado da glicoproteína (gp43) de Paracoccidioides brasiliensis e na flagelina de Salmonella (FliCd)". Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-10022012-144710/.
Pełny tekst źródłaParacoccidioidomycosis (PCM) is a systemic granulomatous disease caused by the dimorphic fungus Paracoccidioides brasiliensis. Approximately 10 million people living in endemic areas may be infected with this fungus and up to 2% of them may develop the disease. Extended periods of chemotherapy are often necessary to treat PCM and relapses occur frequently. PCM vaccines are not yet available for use in humans, but in recent years tests in animal models showed that vaccine strategies are viable. Vaccine formulations based in protein gp43 and a derived peptide P10, representing a CD4+ T cell-specific epitope, have showed promising results as vaccine antigens. In the present work, we tested the properties of a vaccine formulation based on the P10 peptide admixed with the S. enterica flagellin, in combination or not with chemotherapy, as a PCM therapeutic treatment. Treated mice, combined or not with chemotherapy, showed no significant reduction in lung fungal burden. Nonetheless, more organized lung granulomas were observed in animals that received the vaccine formulation.
Lino, Ciro Novaes Rosa. "Caracterização da atividade biológica da serpina salivar AET-7393 de Aedes aegypti". Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/42/42133/tde-18032014-181542/.
Pełny tekst źródłaIn order to successfully feed, hematophagous mosquitoes possess salivary components capable of regulating hemostasis and modulate the host immunity. However, the evaluation of the biological activities of the salivary molecules in the host still needs further investigation. In this study, we intend to characterize the biological activities of the AET-7393, a serpin that is present in the saliva of the females Aedes aegypti mosquitoes. Our data show that the recombinant AET-7393 serpin increases bleeding when inoculated in mice, but apparently this effect is not due to its interference on the coagulation cascade. In addition, AET-7393 is able to inhibit proteinase 3 and enhance the production of IL-1b. Finally, we observed the absence of modulatory effect on macrophage activation or inflammation, and that the presence of host anti-AET-7393 antibodies does not interfere in the life cycle of the mosquitoes.
Pereira, Paulo Vitor Soeiro. "BAY 41-2272: uma ferramenta farmacológica com potencial para o tratamento de infecções". Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/42/42133/tde-18042013-104537/.
Pełny tekst źródłaWe investigated the soluble guanylate cyclase agonist, BAY 41-2272, as an alternative to compensate for failures in of monocytes function. We evaluated the in vitro effect of the drug on human cells lines and peripheral blood cells. The BAY increased expression of CD11b, CD18, CD14, TLR4, TLR2 and CD163 and induce the production of TNF-a, IL-1b, IL-6 and IL-12p70 in THP-1 cells and monocytes. Furthermore, the drug increased NADPH oxidase gene (CYBB, CYBA and NCF2) and protein (gp91phox and p67phox) expression. Also, BAY activates the PKG-dependent NF-kB pathway (p65). More importantly, the drug increased microbicidal activity against S. aureus and C. albicans of monocytes from patients with CGD and MPO deficiency. In animals, BAY 41-2272 induced intense influx of macrophages to the peritoneum and inflammation. BAY potentiated the spreading, phagocytic activity, microbicidal activity, spontaneous production of nitric oxide and PMA-induced hydrogen peroxide release by peritoneal macrophages, increasing host protection against C. albicans. Taken together, our results confirm the potential of BAY 41-2272, or its pathway (sGC / PKG), as an alternative for the development of therapies against infections.
Yoshida, Mutsumi. "Mechanism of biomaterial adjuvant effect phenotype of dendritic cells upon biomaterial contact /". Diss., Available online, Georgia Institute of Technology, 2005, 2005. http://etd.gatech.edu/theses/available/etd-07152005-141108/.
Pełny tekst źródłaBabensee, Julia, Committee Chair ; Andres Garcia, Committee Member ; Mary Marovich, Committee Member ; Barbara Boyan, Committee Member ; Elliot Chaikof, Committee Member ; Cheng Zhu, Committee Member.
Mota, Jennifer A. "The Potential of Coelomocyte Chemotaxis as an Immune Biomarker in the Earthworm, Lumbricus terrestris". Thesis, University of North Texas, 1999. https://digital.library.unt.edu/ark:/67531/metadc2272/.
Pełny tekst źródłaPrestes, Ana Fabíola Rollo de Oliveira. "Avaliação de adjuvantes em novas formulações de vacina tríplice bacteriana". Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-28042009-111641/.
Pełny tekst źródłaThe whole cell pertussis vaccines present some reactogenicity and the acellular, less reactogenic, have prohibitive use due to its high cost. Instituto Butantan developed a less reactogenic whole cell pertussis vaccine (Plow), with low lipopolysaccharide content and an acellular vaccine (Pa), by simple and economic methodology. These preparations, combined to diphtheria and tetanus toxoids (DTPlow and DTPa, respectively), were compared with the traditional DTP, with different adjuvants: vitamin A, pulmonary surfactant, BCG, monophosphoryl lipid A (MPL) and Al(OH)3. The humoral immune response induced in mice by the different vaccine formulations, was similar and independent of the adjuvant used. The vaccines induced balanced levels of IgG1/IgG2a anti-pertussis and DTPlow showed to be less rectogenic, inducing lower levels of serum IL-6. The use of MPL suggested to induce higher protection against nasotracheal colonization in DTPa group and induced IFN-g in the DTP and DTPa groups, suggesting a possible immunemodulatory activity for Th1.
Myschik, Julia, i n/a. "Immunostimulatory lipid implants as delivery systems for model antigen". University of Otago. School of Pharmacy, 2008. http://adt.otago.ac.nz./public/adt-NZDU20080806.114447.
Pełny tekst źródłaJin, Jing, i 金晶. "Proteomic profiling of mycelial extract derived from coriolus versicolor and analysis of their anti-tumor effects in human leukemiccells HL-60". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B41897110.
Pełny tekst źródłaJin, Jing. "Proteomic profiling of mycelial extract derived from coriolus versicolor and analysis of their anti-tumor effects in human leukemic cells HL-60". Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B41897110.
Pełny tekst źródłaJunior, Luiz Antonio Gerardi. "Avaliação da atividade imunomoduladora da microplusina sobre macrófagos murinos in vitro". Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-01122010-110647/.
Pełny tekst źródłaAntimicrobial host defense peptides are components of the innate immune system. They are expressed constitutively or induced by bacterial components, cytokines or the combination of both are expressed constitutively or induced by bacterial components, cytokines, or a combination of both. These peptides have direct action on microorganisms and are potent immunomodulators in mammalian cells. Microplusin is a copper II-chelating antimicrobial peptide from the Tick Rhipicephalus ( Boophilus) microplus, with a molecular mass of 10 204 Da. This peptide has a bacteriostatic activity against Micrococcus luteus, but no activity against E. coli and Candida albicans. The aim of the present work was to analyze the direct effect of microplusin in the culture of murine bone marrow derivated macrophages (BDM) treated or not with LPS or IFN-γ. Our data showed that microplusin had no cytotoxic effect on macrophages, did not stimulate the synthesis of NO and IL-1β, but increased the production of TNF-α and IL-6 after stimulation. The pre-treatment of macrophages with microplusin before LPS addition did not change the level of nitrite, TNF-α or IL-6 in response to LPS. If the microplusin pre-treated macrophages were incubated with IFN-γ there was evidence that microplusin led to significant increase of NO and TNF-α production confirming the pro-inflammatory characteristic of microplusin and its potential against pathogenic microorganisms. This indicates microplusin to be a potential immunomodulating agent of innate immunity and may help to control infections by pathogenic microorganisms.
Santos, Camila Mathias dos. "Avaliação das propriedades imunomoduladoras de toxinas termo-lábeis do tipo II produzidas por Escherichia coli enterotoxigênica (ETEC) administradas por via transcutânea". Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-23102009-113942/.
Pełny tekst źródłaHeat-labile toxins expressed by enterotoxigenic Escherichia coli (LT-I, LT-IIa and LT-IIb) are potent systemic and mucosal adjuvants. These proteins have low identity (<14%) in their B subunits and bind to different receptors, which may result in differential biological properties. The objective of this work was to evaluate the immune response induced by LT-IIa toxin and its pentameric B subunit (LT-IIaB) delivered by transcutaneous and intradermic routes. Initially the proteins of interest were expressed in recombinant E. coli strains, purified and tested for functionality in vitro. In a second moment, the immunomodulatory properties of LT-IIa and LT-IIaB, immunogenicity and adjuvant activity, were evaluated in mouse model. The humoral and cellular immune responses induced against ovalbumin (OVA) used as antigen were determined. The results show that the adjuvant activity of LT-IIa and its B pentamer depends on the route of inoculation and that LT-I and LT-IIa differ both on induction of inflammation and activation of immune responses in mice.
Monaris, Denize. "Avaliação do potencial adjuvante da flagelina FliCi de Salmonella enterica sorovar Thyphimurium no desenvolvimento de uma vacina contra leptospirose". Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-22032011-164924/.
Pełny tekst źródłaLeptospirosis is a global zoonotic disease caused by pathogenic spirochetes of the genus Leptospira. In the present study, we evaluated the adjuvant activity of Salmonella enterica FliCi flagellin in the protective immunity induced by the LigAc and also by six other novel recombinant leptospiral outer membrane lipoproteins (OMP) of Leptospira interrogans serovar Copenhageni. Immunization of hamsters with LigAc or LigAc coadministered with OMPs cocktail, both with FliCi or Al(OH)3, induced robust antibody responses against recombinant proteins, and conferred protection after challenge (86-100%). Moreover, only groups inoculated with the commercial vaccine, bacterin or LigAc coadministered with OMPs cocktail and FliCi as adjuvant showed reduced bacterial load in kidneys (0-28%) with significant enhancement of gene expression of both Th1 and Th2 cytokines. Taken together, our data pave the way for the development of novel vaccine formulations against leptospirosis, using recombinant proteins and FliCi as adjuvant.
Tyavambiza, Caroline. "The antimicrobial and immunomodulatory effects of Cotyledon Orbiculata extracts". Thesis, Cape Peninsula University of Technology, 2018. http://hdl.handle.net/20.500.11838/2721.
Pełny tekst źródłaThe challenge of antimicrobial resistance has increased drastically over the years as more microorganisms are becoming resistant to the available conventional treatments. The burden of antimicrobial resistant infections is intensified by the increase in immunocompromising conditions such as HIV/AIDS and cancer. Due to this challenge, pharmaceutical companies, health sectors and researches are in search of new antimicrobial agents that can solve the problem at hand. Medicinal plants are a reliable source for drug discovery as it is estimated that 25% of modern medicine originated from plants. They have also been used traditionally as sources of medicine in the treatment of many human ailments. Plants can also be applied in the field of nanotechnology. Nanotechnology is a promising field in medicine as it has the potential to offer improved methods for disease diagnostics and therapeutics. The use of plants in nanotechnology brings about biologically friendly nanomaterials. Cotyledon orbiculata is one of the well-known and common plants of South Africa that is used in traditional medicinal practices. The nanotechnology applications as well as the antimicrobial and immunomodulatory effects of this plant were evaluated. The ability of C. orbiculata to synthesize silver nanoparticles was determined. Optimisation of silver nanoparticle synthesis using water extract of C. orbiculata was done at different conditions. The conditions evaluated include, reaction temperature (25 and 70°C), silver nitrate concentration (1 and 3mM), plant extract concentration (1.5, 3 and 6mg/ml) and reaction time. The synthesis of silver nanoparticles using this plant was successful. The optimal conditions for the synthesis of silver nanoparticles using C. orbiculata were 3mg/ml of the C. orbiculata extract, 3mM silver nitrate at a reaction temperature of 70°C for 2 hours. Under these conditions, spherical, crystalline nanoparticles with sizes of 20-40nm were produced. The antimicrobial and immunomodulatory properties of C. orbiculata extracts and silver nanoparticles were evaluated. Antimicrobial activity was evaluated against Staphylococcus aureus, Staphylococcus epidermidis, Methicillin resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Candida albicans, using the micro-dilution assay to determine the minimum inhibitory concentration (MIC). The results obtained revealed that all extracts of C. orbiculata have antimicrobial properties against all the microorganisms tested. The MICs of the extracts ranged from 3.13 to 50mg/ml and the MBC/MFC from 6.25 to >100mg/ml. The methanol extract exhibited better antimicrobial activity in comparison to the others extracts whereas the water extract had better antifungal properties. The chloroform extract showed the lowest activity in both antibacterial and antifungal studies. Silver nanoparticles also exhibited antimicrobial activity against all the microorganisms tested. It’s MICs against these microorganisms ranged from 5–80μg/ml and MBC/MFC from 20-160μg/ml. The silver nanoparticles were highly active than the water extract against both the bacteria and the fungi. Immunomodulatory effects of the plant extracts and silver nanoparticles were determined by evaluating cytokine production using the enzyme linked immunoassay (ELISA) assay. All the extracts and silver nanoparticles of C. orbiculata were found to have anti-inflammatory properties. The water extracts showed more anti-inflammatory activity against the cytokines than the other extracts. However the silver nanoparticles were more active than the water extract. The findings from this study confirmed that C. orbiculata have antimicrobial and immunomodulatory effects. This provided scientific evidence of the traditional use of this plant in the treatment of skin infections and inflammatory conditions.
Santos, Camila Mathias dos. "Toxinas termo-lábeis (LTs) do tipo II de Escherichia coli enterotoxigênica (ETEC): efeito adjuvante e atividade inflamatória". Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-15122014-125225/.
Pełny tekst źródłaThis work has made significant advances in the understanding of the potential of type II heat-labile toxins (LT-IIs) as vaccine adjuvants by intradermal and transcutaneous route. The generated data indicate that native forms of LT-IIb and LT-IIc act as potent vaccine adjuvants when intradermally injected inducing antigen-specific immune responses, as measured by the generation of systemic serum antibody (IgG) and activation of cytotoxic CD8+ T lymphocytes. Besides the adjuvant effects, LT-IIs show reduced side effects, measured by the lesser edema formation and reduced leukocytes migration to the site of injection, in comparison to LT-I. The results also indicate that the adjuvant activity of LTs applied transcutaneously may be related to the the ganglioside GM1 binding property since the LT-IIb toxin, unable to interact with this receptor, has no adjuvant effect by this route. The presented data set opens prospects for the employment of native LT-IIs as parenteral adjuvants in vaccines for animals and humans.
Yang, Xiaotong, i 楊曉彤. "The anticancer mechanisms of polysaccharide peptide (PSP) derived fromthe Chinese medicinal fungus coriolus versicolor". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B31246229.
Pełny tekst źródłaFerreira, Líllian das Neves. "Capacidade imunomoduladora de extratos etanólico de própolis verde e aquoso de própolis marrom em camundongos inoculados com antígenos múltiplos". Universidade Federal de Pelotas, 2009. http://repositorio.ufpel.edu.br/handle/ri/2549.
Pełny tekst źródłaThe vaccines with multiple antigens have represented a major advance in the immunization of pets and production animals, since they represent economies of time, cost, and stress. However, the efficiency of these vaccines have sometimes been questioned and the choice of an appropriate adjuvant can mean a breakthrough in multiple immunizations. In this context, propolis, which is drawing the attention of many researchers due to its bioactive properties, emerges as a possible candidate, because many studies have demonstrated its stimulating activity on the humoral and cellular immune system. As studies have shown adjuvant activity in inactivated and monovalent vaccines, but there are no reports on its action on multiple and attenuated vaccines, this study aimed at evaluating the immunomodulatory capacity of ethanolic extract of green propolis and aqueous extract of brown propolis, against attenuate parvovirus canine (CPV) and inactivated canine coronavirus (CCoV), when associated with a vaccine composed of multiple antigens. Green propolis stimulated the production of antibodies against CPV in animals inoculated with the highest concentration of antigen (3.0 x106 TCID50) co-administered with 400 mg/dose of its ethanolic extract. The addition of 400 mg/dose of aqueous extract of brown propolis to different concentrations of commercial antigens (0.75, 1.5 e 3.0 x 106 TCID50) increased the humoral immune response against CPV in all groups. Regarding CCoV, green propolis showed no adjuvant activity. Already titration of the antibodies showed an increase in animals inoculated with 1.5 and 3.0 x106 TCID50 of antigens co-administered with aqueous extract of brown propolis. The cellular response was assessed by the expression of messenger RNA (mRNA) for interferon gamma (INFı), revealing that the green propolis showed no adjuvant activity, while the brown propolis increased the production of this cytokine against CPV in animals inoculated with 0.75 and 3.0 x106 TCID50 of antigens. The production of INF-ı specific to CCoV revealed properties immunostimulating agent of green propolis in concentrations of 0.75 and 3.0 x106 TCID50 of antigens and of brown propolis at a dose of vaccine of 3.0 x106 TCID50. The results presented in this study demonstrated that the ethanol extract of green propolis increased humoral response against CPV and cellular response against CCoV.And the aqueous extract of brown propolis has adjuvant activity on the humoral and cellular immune responses against the attenuated CPV and inactivated CCoV, when incorporated into a multiple vaccine. The use of propolis may contribute to the effectiveness of multiple vaccines due its stimulating activity to both the humoral and cellular immune responses, which justifies more research in that area.
As vacinas com antígenos múltiplos têm representado um grande avanço na imunização de animais de companhia e de produção, já que aliam economias de tempo, custo, manejo e estresse. No entanto, a eficiência dessas vacinas tem sido por vezes questionada e a escolha de um adjuvante adequado pode significar um avanço nas imunizações múltiplas. Nesse contexto, a própolis, que vem chamando a atenção de muitos pesquisadores devido as suas propriedades bioativas, surge como uma possível candidata, pois muitas pesquisas vêm demonstrando suas atividades moduladoras sobre o sistema imune humoral e celular. Como estudos já demonstraram atividade adjuvante em vacinas inativadas e monovalentes, mas ainda não existem relatos sobre sua ação em vacinas múltiplas e atenuadas, o objetivo desse trabalho foi avaliar a capacidade imunomoduladora de extratos etanólico de própolis verde e aquoso de própolis marrom, contra o parvovírus canino (CPV) atenuado e o coronavírus canino (CCoV) inativado, quando associados com uma vacina composta por antígenos múltiplos. A própolis verde estimulou à produção de anticorpos contra o CPV nos animais inoculados com a maior concentração dos antígenos (3,0x106 TCID50) co-administrados com 400 μg de seu extrato etanólico. A adição de 400 μg/dose de extrato aquoso de própolis marrom a diferentes concentrações dos antígenos comerciais (0,75, 1,5 e 3,0 x 106 TCID50) incrementou a resposta imune humoral contra o CPV em todos os grupos. Com relação ao CCoV, a titulação dos anticorpos não revelou atividade adjuvante da própolis verde, no entanto foi verificado aumento na resposta humoral nos animais inoculados com 1,5 e 3,0x106 TCID50 dos antígenos co-administrados com extrato aquoso de própolis marrom. A resposta celular foi avaliada pela expressão de RNA mensageiro (mRNA) para interferon gamma (INF-ı), revelando que a própolis verde não apresentou ação imunoestimulante, já a própolis marrom incrementou a produção dessa citocina contra CPV nos animais inoculados com 0,75 e 3,0x106 TCID50 dos antígenos. A produção de INF-ı específico para CCoV revelou propriedade imunoestimulante da própolis verde nas concentrações de 0,75 e 3,0 x106 TCID50 dos antígenos e da própolis marrom na dose de vacina de 3,0x106 TCID50. Os resultados apresentados nesse estudo demonstraram que o extrato etanólico de própolis verde incrementou a resposta humoral contra o CPV e a celular contra o CCoV. Já o extrato aquoso de própolis marrom apresentou atividade adjuvante sobre as respostas imunes humoral e celular, contra o CPV atenuado e o CCoV inativado, quando incorporado a uma vacina múltipla. O uso da própolis pode contribuir para a eficácia de vacinas múltiplas devido à atividade estimulante tanto sobre a resposta imune humoral como celular, justificando mais pesquisas nessa área.
Halpern, Melissa Dale. "The in vivo and in vitro effects of diethyldithiocarbamate on autoimmune New Zealand Black/White F₁ hybrid, MRL/Mp-lpr/lpr and related and normal murine strains". Diss., The University of Arizona, 1989. http://hdl.handle.net/10150/184940.
Pełny tekst źródłaCarvalho, Luciana Vieira. "Efeito da sílica nanoestruturada SBA-15 na apresentação antigênica e na resposta imune". Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/42/42133/tde-11012011-134449/.
Pełny tekst źródłaThe SBA-15 silica is a polymer that due to its physico-chemical and structural properties has shown adjuvant effect, carrying, protecting and delivering antigens. In this study was evaluated the effect of SBA15 in the phagocytic activity of macrophages; recruitment of cells to lymphoid organs; expression of MHC class II and co-stimulatory molecules by the APCs, and the influence of contact time between immunogen:SBA-15 in potential for absorption and generation of antibodies. In vitro experiments showed that different concentrations of SBA-15 do not affect the morphology or macrophage activity. SBA-15 induces the recruitment of macrophages, dendritic cells, CD4+, CD8+ and B220+ to the draining lymph nodes, increasing the expression of co-stimulatory molecules. Analyses of antibody production showed that the contact immunogen:silica is important for adsorption and improves the immune response. These results suggest that the adjuvanticity of SBA-15 is related to its ability in antigens adsorption and release to APCs, directly influencing the immune response.
Rivillas, Carolina Salcedo. "Caracterização dos efeitos imunomoduladores e adjuvanticidade da flagelina Hag de Bacillus subtilis". Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-19092012-091636/.
Pełny tekst źródłaFlagellin of gram negatives bacteria actives the innate immune system resulting in adjuvants effects against coadministrated antigens. However, there is not information about immunological properties of flagellins produced by gram positive bacteria. This study aimed the evaluation of immunomodulator effects of Hag flagellin from B. subtilis. Vaccine formulations based in purified Hag and Ovalbumine protein (OVA) were administrated intranasal (i.n.) and subcutaneously (s.c.) in Balb/C and C57BL/6 mice and then the population of B and T lymphocytes were monitored for production of antibodies and citocines and/or surface markers expression. Results showed the immunomodulatory properties of Hag in both mice lineages regarding the induction of antigen specific antibodies when immunized with OVA and Hag by i.n. and s.c. via. Most promising results concerning the adjuvant effects observed on T cells activation were found in C57BL/6 mice. The results obtained open future perspectives for the study of the potential use of B. subtilis Hag flagellin as adjuvant in vaccines.