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Latimer, Christine. "The invisible view betwixt and between : exegesis submitted in partial fulfilment of requirements for the degree of Masters of Art & Design, AUT University, November, 2008". Click here to access this resource online, 2008. http://hdl.handle.net/10292/456.
Pełny tekst źródłaPark, Adam C. "Performing as mapping : an examination of the role of site-specific performance practice as a methodology to map and/or re-imagine sites of urban regeneration". Thesis, University of Sheffield, 2014. http://etheses.whiterose.ac.uk/7718/.
Pełny tekst źródłaHuber, Florian Gerhard. "Site-Resolved Imaging with the Fermi Gas Microscope". Thesis, Harvard University, 2014. http://dissertations.umi.com/gsas.harvard:11595.
Pełny tekst źródłaPhysics
Levenderis, Leandra Paula Rosa. "Site Specific Opera : a Re-imagined Magic Flute as a Catalyst to the Narration of Fort Daspoortrand Heritage". Diss., University of Pretoria, 2018. http://hdl.handle.net/2263/63627.
Pełny tekst źródłaMini-dissertation Mint(Prof)--University of Pretoria, 2018.
Architecture
MInt(Prof)
Unrestricted
Held, Christoph. "Creating 3D models of cultural heritage sites with terrestrial laser scanning and 3D imaging". Master's thesis, University of Cape Town, 2012. http://hdl.handle.net/11427/12076.
Pełny tekst źródłaIncludes bibliographical references.
The advent of terrestrial laser-scanners made the digital preservation of cultural heritage sites an affordable technique to produce accurate and detailed 3D-computermodel representations for any kind of 3D-objects, such as buildings, infrastructure, and even entire landscapes. However, one of the key issues with this technique is the large amount of recorded points; a problem which was even more intensified by the recent advances in laser-scanning technology, which increased the data acquisition rate from 25 thousand to 1 million points per second. The following research presents a workflow for the processing of large-volume laser-scanning data, with a special focus on the needs of the Zamani initiative. The research project, based at the University of Cape Town, spatially documents African Cultural Heritage sites and Landscapes and produces meshed 3D models, of various, historically important objects, such as fortresses, mosques, churches, castles, palaces, rock art shelters, statues, stelae and even landscapes.
Fahringer, Peter E. "Geophysical investigations of near-surface mine sites in northern West Virginia". Morgantown, W. Va. : [West Virginia University Libraries], 1999. http://etd.wvu.edu/templates/showETD.cfm?recnum=1087.
Pełny tekst źródłaTitle from document title page. Document formatted into pages; contains ix, 130 p. : ill. (some col.), maps (some col.). Includes abstract. Includes bibliographical references (p. 128-130).
Papachristou, Maria, George Kastis, Petros Stavrou, Stavros Xanthopoulos, Lars Furenlid, Ioannis Datseris i Penelope Bouziotis. "Radiolabeled methotrexate as a diagnostic agent of inflammatory target sites: A proof-of-concept study". SPANDIDOS PUBL LTD, 2017. http://hdl.handle.net/10150/626570.
Pełny tekst źródłaConnell, John J. "Selective permeabilisation of the blood-brain barrier at sites of metastasis". Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:8c027208-8ea6-4de4-be78-ccead5121509.
Pełny tekst źródłaTickhill, Justin David. "The Virtual Pig Head: Digital Imaging in Cephalic Anatomy". Ohio University / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1187634238.
Pełny tekst źródłaSoebbing, Samantha Lynn. "Incorporation of histidine-rich metal-binding sites onto small protein scaffolds implications for imaging, therapeutics, and catalysis /". Diss., University of Iowa, 2008. http://ir.uiowa.edu/etd/37.
Pełny tekst źródłaSun, Ruixue. "Near Surface High Resolution Seismic Imaging of Glacial Deposits in Sweden at the Heby and Marsta Sites". Licentiate thesis, Uppsala universitet, Institutionen för geovetenskaper, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-417104.
Pełny tekst źródłaFlores, Orozco Adrián [Verfasser]. "Characterization of contaminated sites and monitoring of processes accompanying bioremediation using spectral induced polarization imaging / Adrián Flores Orozco". Bonn : Universitäts- und Landesbibliothek Bonn, 2012. http://d-nb.info/1044082607/34.
Pełny tekst źródłaSaakana, Amon Saba. "Sites of conflict : identity, sexuality, reproduction; European mythological imaging of the African on the London stage, 1908-1939". Thesis, Goldsmiths College (University of London), 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321613.
Pełny tekst źródłaSaleem, Muhammad Umar. "2D Reflection Seismic Imaging at a Quick-clay Landslide Site, in Southwest Sweden". Thesis, Uppsala universitet, Geofysik, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-186988.
Pełny tekst źródłaAlcalde, Martín Juan. "3D seismic imaging and geological modeling of the Hontomin CO2 storage site, Spain". Doctoral thesis, Universitat de Barcelona, 2014. http://hdl.handle.net/10803/284824.
Pełny tekst źródłaEsta tesis tiene como objetivo la caracterización geológica 3D de la Planta de Desarrollo Tecnológico para el Almacenamiento Geológico de CO2 de Hontomín (Burgos). Esta caracterización se ha llevado a cabo mediante el procesado y la interpretación de datos de sísmica de reflexión 3D adquiridos para ese propósito en verano de 2010.
Slattery, Kristen Elizabeth. "A GROUND-PENETRATING RADAR IMAGING OF SCHOENBRUNN VILLAGE ARCHAEOLOGICAL SITE, TUSCARAWAS COUNTY, OHIO". Kent State University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=kent1321896945.
Pełny tekst źródłaBalasubramaniam, Aswin. "Applications of Small Unmanned Aerial Systems (sUAS) and Photogrammetry to Monitor and Inspect Structural Health and Construction Sites". University of Cincinnati / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1592133796045396.
Pełny tekst źródłaMatsui, Joy Tamiko. "Development of image processing tools and procedures for analyzing multi-site longitudinal diffusion-weighted imaging studies". Diss., University of Iowa, 2014. https://ir.uiowa.edu/etd/4690.
Pełny tekst źródłaAhlgren, Sara. "Molecular Radionuclide Imaging Using Site-specifically Labelled Recombinant Affibody Molecules : Preparation and Preclinical Evaluation". Doctoral thesis, Uppsala universitet, Institutionen för medicinska vetenskaper, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-122177.
Pełny tekst źródłaSchumacher, Dominik. "Site-specific functionalization of antigen binding proteins for cellular delivery, imaging and target modulation". Doctoral thesis, Humboldt-Universität zu Berlin, 2017. http://dx.doi.org/10.18452/18547.
Pełny tekst źródłaAntibodies and antigen binding proteins conjugated to fluorophores, tracers and drugs are powerful molecules that enabled the development of valuable diagnostic and therapeutic tools. However, the conjugation itself is highly challenging and despite intense research efforts remains a severe bottleneck. In addition to that, antibodies and antigen binding proteins are often not functional within cellular environments and unable to penetrate the cellular membrane. Therefore, their use is limited to extracellular targets leaving out a vast number of important antigens. Both limitations are core aspects of the presented thesis. With Tub-tag labeling, a novel and versatile method for the site-specific functionalization of biomolecules and antigen binding proteins was developed expanding the toolbox of protein functionalization. The method is based on the microtubule enzyme tubulin tyrosine ligase. Tub-tag labeling was successfully applied for the site-specific functionalization of different proteins including antigen binding nanobodies which enabled confocal microscopy, protein enrichment and super-resolution microscopy. In addition to that, cell permeable antigen binding nanobodies have been generated constituting a long thought goal of tracking and manipulating intracellular targets by in vitro functionalized antigen binding proteins. To achieve this goal, two different nanobodies were functionalized at their C-terminus with linear and cyclic cell-penetrating peptides using expressed protein ligation. These peptides triggered the endocytosis independent uptake of the nanobodies with immediate bioavailability. Taken together, Tub-tag labeling and the generation of cell-permeable antigen binding nanobodies strongly add to the functionalization of antibodies and their use in biochemistry, cell biology and beyond.
Tanguy, Quentin. "Design and fabrication of a MEMS scanner for OCT imaging endo-microscopic probe". Thesis, Bourgogne Franche-Comté, 2018. http://indexation.univ-fcomte.fr/nuxeo/site/esupversions/8d6ab7ed-eac5-4e11-997b-f9e9af4e8808.
Pełny tekst źródłaThe work presented in this manuscript consists in the conception of an electro-thermo-mechanical optical micro-scanner. In a first time, a design and a model are proposed and the micro-device was fabricated, characterized and a draft of integration onto a Mirau micro-interferometer was carried out in order to build an endoscopic probe for the detection of early stage gastrointestinal cancers using an imaging technique called Optical Coherence Tomography (OCT). The very contribution of the thesis is the development and the fabrication of micro actuators driven electrothermally to control the angular position a micro mirror plate conceived in this purpose. Different architectures of micro-scanners are proposed to best comply with the specifications determined by both the optical expectations and the medical standards requirements of the final apparatus emph{in vivo}.This work, part of a project called textquote{DEMO4} was financed by the French LabEx Action funding source in cooperation between the departments AS2M and MN2S of the French research institute Femto-ST as well as the university of Florida in the USA to deliver a first demonstrator of endoscopic OCT probe.Several batches of fabrication were realized my means of techniques of ac{CMOS}-based microfabrication in exchange between Femto-ST and the university of Florida before being assembled (for lack of monolithic integration capability) onto a micro-interferometer using automated processes specifically developed in this purpose.Finally, an experimental setup is proposed to characterize in order to evaluate the performances achieved and initiate the first tests of optical imaging using the micro-system
Valliant, John Fitzmaurice. "Synthesis and NMR spectroscopy of tripeptide derived biomolecules for site specific radiopharmaceuticals". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0021/NQ30178.pdf.
Pełny tekst źródłaSom, Subhojit. "Topics in Sparse Inverse Problems and Electron Paramagnetic Resonance Imaging". The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1282135281.
Pełny tekst źródłaUttamapinant, Chayasith. "Cellular delivery and site-specific targeting of organic fluorophores for super-resolution imaging in living cells". Thesis, Massachusetts Institute of Technology, 2013. http://hdl.handle.net/1721.1/79263.
Pełny tekst źródłaVita. Cataloged from PDF version of thesis.
Includes bibliographical references.
Recent advances in super-resolution fluorescence microscopy have pushed the spatial resolution of biological imaging down to a few nanometers. The key element to the development of such imaging modality is synthetic organic fluorophores with suitable brightness and photostability. However, organic fluorophores are very difficult to use in live cells because of their chemical compositions. Many excellent fluorophores, such as cyanine and Alexa Fluor dyes, are highly charged with sulfonate groups and do not cross the plasma membrane. Even if the fluorophores get inside cells, there exist few methods that can be used to target these nongenetically encoded probes to specific cellular proteins with high specificity and minimal interference. We describe herein the development of new methods for cellular delivery and sitespecific targeting of organic fluorophores to proteins in living cells. Building on our lab's previous work on engineering new substrate specificity for E. coli lipoic acid ligase (LplA), we created a mutant ligase that catalyzes covalent conjugation of a 7-hydroxycoumarin fluorophore onto a 13-amino acid peptide substrate, called LAP. We showed that enzymatic fluorophore ligation is compatible with the living cell interior and is highly specific for LAP fusion proteins. To extend the repertoire of fluorophores targetable by LplA inside cells, we devised a two-step labeling approach based on enzymatic azide ligation, followed by chemoselective derivatization with any membrane-permeable fluorophore via strain-promoted cycloaddition. As an auxiliary tool for enzymatic probe ligation, we also developed a very efficient and biocompatible variant of copper-catalyzed azide-alkyne cycloaddition that can be used for modification of cell-surface proteins. To overcome the lack of membrane permeability of sulfonated fluorophores, we identified a chemical reaction that efficiently masks charged sulfonate groups as esterase-labile sulfonate esters. Such masked sulfonated fluorophores enter cells readily and can be sitespecifically targeted to intracellular proteins. Our efforts in developing protein labeling and fluorophore delivery methods culminated in their application to super-resolution imaging of cellular proteins in living cells.
by Chayasith Uttamapinant.
Ph.D.
Lampinen, Björn. "Protocol optimization of the filter exchange imaging (FEXI) sequence and implications on group sizes : a test-retest study". Thesis, Uppsala universitet, Institutionen för informationsteknologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-196327.
Pełny tekst źródłaWhite, Katharine Alice. "Rational design and directed evolution of probe ligases for site-specific protein labeling and live-cell imaging". Thesis, Massachusetts Institute of Technology, 2012. http://hdl.handle.net/1721.1/78438.
Pełny tekst źródłaCataloged from PDF version of thesis.
Includes bibliographical references.
Chemical fluorophores have superior photophysical properties to fluorescent proteins and are much smaller. However, in order to use these probes for live-cell protein imaging, highly specific labeling methods are required. Here, we will describe three efforts to re-engineer the E. coli enzyme, lipoic acid ligase (LplA), to catalyze the ligation of small-molecule probes onto recombinant proteins. We call this collection of methods the PRIME (PRobe Incorporation Mediated by Enzymes) methodologies. First, we describe the structure-guided mutagenesis of LplA and the identification of an LplA variant that can ligate a blue coumarin fluorophore onto a 13-amino acid LplA acceptor peptide (LAP2). This "coumarin ligase" can be used to image cellular proteins with high specificity, sensitivity, and minimal perturbation of the biology of the protein of interest. We also demonstrate how subpopulations of a protein of interest can be labeled using genetically targeted coumarin ligase. Second, we describe our attempts to use yeast display evolution and fluorescence activated cell sorting (FACS) to evolve a truncated LplA enzyme. The original truncated enzyme had severely decreased activity for LplA's natural substrate, lipoic acid. We created a 107 library of LplA mutants and, after four rounds of selection, produced a truncated LplA mutant with lipoylation activity equivalent to full-length LplA. We next sought to evolve activity for an unnatural small molecule probe, but found that this strategy was limited by both increased hydrophobic probe sticking when using the truncated enzyme and some enzyme-dependent nonspecificity. Finally, from a library of 107 LplA mutants, we evolved a full-length LplA capable of ligating an unnatural picolyl azide (pAz) substrate. We demonstrated improved activity of the "pAz ligase" in the secretory pathway and cell surface, two regions where coumarin ligase is inactive. This enzyme can also be used to image cell surface protein-protein interactions as well as label proteins as they are trafficked through the endoplasmic reticulum. These probe ligases will be useful tools for cell biologists interested in studying protein function or protein-protein interactions in the context of living cells.
by Katharine Alice White.
Ph.D.
Miyachi, Shigeru, Takashi Izumi, Noriaki Matsubara, Osamu Hososhima, Yuko Tsurumi i Arihito Tsurumi. "Virtual Histology Analysis of Carotid Atherosclerotic Plaque: Plaque Composition at the Minimum Lumen Site and of the Entire Carotid Plaque". Wiley-Blackwell, 2013. http://hdl.handle.net/2237/17694.
Pełny tekst źródłaPerols, Anna. "Site-specific labeling of affinity molecules for in vitro and in vivo studies". Doctoral thesis, KTH, Proteinteknologi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-152349.
Pełny tekst źródłaQC 20140929
Yang, Yidong. "Monitoring cell infiltration into the myocardial infarction site using micrometer-sized iron oxide particles-enhanced magnetic resonance imaging". Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/41151.
Pełny tekst źródłaKampmeier, Florian [Verfasser]. "Site directed modification of recombinant antibody fragments for in vivo fluorescence imaging and targeted drug delivery / Florian Kampmeier". Aachen : Hochschulbibliothek der Rheinisch-Westfälischen Technischen Hochschule Aachen, 2011. http://d-nb.info/1014263816/34.
Pełny tekst źródłaChen, Juan [Verfasser], Thomas [Akademischer Betreuer] Arentz i Amir S. [Akademischer Betreuer] Jadidi. "Extent and spatial distribution of left atrial arrhythmogenic sites, late gadolinium enhancement at magnetic resonance imaging, and low-voltage areas in patients with persistent atrial fibrillation: comparison of imaging vs. electrical parameters of fibrosis and arrhythmogenesis". Freiburg : Universität, 2019. http://d-nb.info/1194312691/34.
Pełny tekst źródłaYordkayhun, Sawasdee. "2D and 3D Seismic Surveying at the CO2SINK Project Site, Ketzin, Germany: The Potential for Imaging the Shallow Subsurface". Doctoral thesis, Uppsala University, Department of Earth Sciences, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-9273.
Pełny tekst źródłaSeismic traveltime inversion, traveltime tomography and seismic reflection techniques have been applied for two dimensional (2D) and three dimensional (3D) data acquired in conjunction with site characterization and monitoring aspects at a carbon dioxide (CO2) geological storage site at Ketzin, Germany (the CO2SINK project). Conventional seismic methods that focused on investigating the CO2 storage and caprock formations showed a poor or no image of the upper 150 m. In order to fill this information gap, an effort on imaging the shallow subsurface at a potentially risky area at the site is the principal goal of this thesis.
Beside this objective, a seismic source comparison from a 2D pilot study for acquisition parameter testing at the site found a weight drop source suitable with respect to the signal penetration, frequency content of the data and minimizing time and cost for 3D data acquisition.
For the Ketzin seismic data, the ability to obtain high-quality images is limited by the acquisition geometry, source-generated noise and time shifts due to near-surface effects producing severe distortions in the data. Moreover, these time shifts are comparable to the dominant periods of the reflections and to the size of structures to be imaged. Therefore, a combination of seismic refraction and state-of-the-art processing techniques, including careful static corrections and more accurate velocity analysis, resulted in key improvements of the images and allowed new information to be extracted. The results from these studies together with borehole information, hydrogeologic models and seismic modeling have been combined into an integrated interpretation. The boundary between the Quaternary and Tertiary unit has been mapped. The internal structure of the Quaternary sediments is likely to be complicated due to the shallow aquifer/aquitard complex, whereas the heterogeneity in the Tertiary unit is due to rock alteration associated with fault zones. Some of the major faults appear to project into the Tertiary unit. These findings are important for understanding the potentially risky anticline crest and can be used as a database for the future monitoring program at the site.
Piras, Monica. "Design of novel αvβ3 ligands as probes for imaging of tumour angiogenesis and site-directed delivery of cytotoxic drugs". Thesis, University of Aberdeen, 2014. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=225672.
Pełny tekst źródłaFader, Amelia Erin. "Integration of surface seismic waves, laboratory measurements, and downhole acoustic televiewer imaging, in geotechnical characterization: Ogden, KS". Thesis, Kansas State University, 2012. http://hdl.handle.net/2097/15118.
Pełny tekst źródłaDepartment of Geology
Abdelmoneam Raef
Geotechnical site characteristics are a function of the subsurface elastic moduli and the geologic structures. This study integrates borehole, surface and laboratory measurements for a geotechnical investigation that is focused on investigating shear-wave velocity (Vs) variation and its implication to geotechnical aspects of the Ogden test site in eastern Kansas. The area has a potential of seismicity due to the seismic zone associated with the Nemaha formation where earthquakes pose a moderate hazard. This study is in response to recent design standards for bridge structures require integrating comprehensive geotechnical site characterization. Furthermore, evaluation of dynamic soil properties is important for proper seismic response analysis and soil modeling programs. In this study, near surface geophysical site characterization in the form of 2D shear-wave velocity (Vs) structure that is compared with laboratory measurements of elastic moduli and earth properties at simulated in situ overburden pressure conditions and synergy with downhole Acoustic Televiewer time and amplitude logs, proved very robust “validated” workflow in site characterization for geotechnical purposes. An important component of a geotechnical site characterization is the evaluation of in-situ shear modulus, Poisson’s ratio and reliable and accurate elastic modulus ([lambda]) and shear modulus ([mu]) estimates are important in a good geotechnical site characterization. The geophysical site characterization, undertaken in this study, will complement and help in extrapolating drilling and core-based properties deduced by the geotechnical engineers interested at the test site.
Lescure, Robin. "Développement d’azaBODIPYs fonctionnalisables pour la conception de sondes d’imagerie bimodale et d’agents théranostiques". Thesis, Bourgogne Franche-Comté, 2020. https://nuxeo.u-bourgogne.fr/nuxeo/site/esupversions/21e2771a-9c75-46d7-9377-50e8f2536c32.
Pełny tekst źródłaThe in vivo use of optical imaging is still limited by the lack of near infrared emitting probes. This thesis work focuses on the optimization and valorization of a water-soluble fluorescent platform whose optical properties enable an in vivo use. Two distinct applications were investigated for this WazaBY (Water-soluble azaBODIPY) platform: use as a PET (or SPECT) / optical bimodal probe, and as a theranostic agent. Concerning the first project, we were able to develop a targetted SPECT/optical bimodal probe, which was radiometallated with indium 111. Using xenografted murine models, we were able to show a clear accumulation of the probe in the tumor 24 hours after injection. Moreover, the probe was validated as a contrast agent for fluorescence guided surgery experiment. The second project of this thesis began by the synthesis of a first generation of gold based theranostic agents. The goal was to develop a new therapeutic complex, which can be tracked in vitro and in vivo thanks to optical imaging. In vitro preliminary results showed that the theranostics displayed a cytotoxicity comparable to auranofin on the tested cell lines (4T1, MDA MB 231, CT26 and SW480). A second part of this project focused on the develoment of « smart » probes for a theranostic use. Those probes are designed to undergo photophysical properties changes, when their metallic centre, responsible for the therapeutic role, is released. Two molecules were synthesized, both displaying an on/off behavior
Winkelmann, Kay Hasko. "On the applicability of imaging spectrometry for the detection and investigation of contaminated sites with particular consideration given to the detection of fuel hydrocarbon contaminants in soil". kostenfrei, 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=976701782.
Pełny tekst źródłaCanovas, Coline. "Développement de stratégies de bioconjugaison innovantes : Application à l’élaboration d’agents d’imagerie moléculaire". Thesis, Bourgogne Franche-Comté, 2018. http://www.theses.fr/2018UBFCK058.
Pełny tekst źródłaThe preparation of targeted imaging agents suitable for cancer diagnostic involves the coupling of an imaging probe to a vector, generally a protein, able to specifically target cancerous tissues. Unfortunately, this bioconjugation step is often problematic. Indeed, proteins present a large variety of reactive functions that can potentially interfere with the coupling reaction. The purpose of this work is the design of innovative selective bioconjugation strategies that can facilitate the synthesis of molecular imaging agents.Our research led to the development of a simple and efficient approach allowing the modification of a protein with a fluorophore by using a site-specific bioconjugation reaction. Thanks to this technique, it is possible to obtain near infrared fluorescent agents in a single step.We also focused our work on the design of bimodal nuclear/fluorescent imaging tracers based on protein vectors. These bimodal imaging agents show high potential for numerous medical applications. However, their synthesis requires the coupling of two distinct imaging molecules to the protein, which makes the bioconjugation step even more challenging. In this context, we developed different strategies suitable for the site-specific dual-modification of proteins. These approaches are based on a highly modular trifunctional platform, dichlorotetrazine. Several bimodal nuclear/optical imaging agents were obtained and shown convenient in vivo diagnostic properties.This thesis also presents the elaboration of a new PSMA-specific radiotracer able to detect prostate cancer. The promising results obtained during the preclinical evaluation of this compound labeled with copper-64, make it an interesting candidate for the diagnosis of prostate cancer by positron emission tomography in human
Lin, Chi-Wang. "Methodology development for imaging histone modifications and for site-specific protein labeling in vitro and on the surface of living cells". Thesis, Massachusetts Institute of Technology, 2006. http://hdl.handle.net/1721.1/37605.
Pełny tekst źródłaThis electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.
Includes bibliographical references (leaves 187-207).
A new methodology for monitoring post-translational modifications of histone H3 in living cells was developed using genetically encoded fluorescent reporters. These reporters were constructed for sensing histone phosphorylation and methylation by fluorescence resonance energy transfer (FRET). These reporters are four-part chimeric proteins with the domains from N- to C-terminus as follows: a cyan fluorescent protein (CFP), a phosphoserine recognition domain (14-3-3 tau) or a methyllysine recognition domain (HP1 or Pc chromodomain), a peptide whose sequence corresponds to the Nterminus of histone H3, and a yellow fluorescent protein. The histone phosphorylation reporter exhibited a 25 % YFP/CFP emission ratio increase upon phosphorylation in vitro by Msk-1 kinase. Site-directed mutagenesis studies suggested that Ser28 phosphorylation gave rise to the reporter FRET response. When tested in living HeLa cells, the reporter exhibited a rapid increase in the emission ratio 5-15 min prior to the nuclear membrane breakdown and the FRET peaked during cell division. Another reporter, in which the 14-3-3 tau and the H3 peptide were swapped, exhibited the FRET response to both Ser10 and Ser28 phosphorylation. Two methylation reporters, K9 and K27, were constructed for sensing H3-lysine9 and H3-lysine27 methylation.
(cont.) The reporters gave 60 % (K9) and 28 % (K27) emission ratio changes after in vitro methylation, catalyzed by the histone methyltransferase vSET. Applying the K9 reporter in cells showed different levels of reporter FRET in fibroblasts either expressing or lacking methyltransferases Suv39h1 and Suv39h2. Site-specific incorporation of biophysical probes onto cell surface proteins is critical for the study of protein trafficking. One general solution to achieve labeling specificity is the use of enzymes for the ligation of probes to a substrate peptide tagged onto a protein of interest. Transglutaminase incorporates amine probes to cell surface proteins expressing a 6- or 7-amino acid consensus sequence (Q-tag). A variety of probes such as biotin cadaverine and fluorescein cadaverine were incorporated to Q-tag-CFP and Q-tag-EGF receptor constructs expressed on the surface of living HeLa and HEK cells. The NF?B p50 transcription factor fused to a Q-tag was labeled with a benzophenone photo-affinity probe in vitro. Upon UV irradiation, elevated levels of p50 homodimerization were observed in the presence of DNA or the interacting protein myotrophin.
by Chi-Wang Lin.
Ph.D.
Bookjans, Eva M. "Relative number squeezing in a Spin-1 Bose-Einstein condensate". Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/37148.
Pełny tekst źródłaLarivière, Mélusine. "Nanoparticles functionalized with human antibodies for multimodal molecular imaging of atherosclerosis". Thesis, Bordeaux, 2016. http://www.theses.fr/2016BORD0389/document.
Pełny tekst źródłaBecause cardiovascular diseases are the leading cause of death in the world, providing clinicians with reliable and straightforward imaging techniques to identify "vulnerable" patients from the general population appears like the Holy Grail of the cardiovascular field. Atherosclerosis, identified as the underlying condition for most acute cardiovascular events, is characterized by the constitution of a lipidrich atheroma plaque, driven both by excess cholesterol and inflammation, which eventual rupture triggers clotting into the blood flow. It involves a wealth of cellular and molecular actors, which are so many potential markers for molecular imaging, aiming at deciphering how to warn clinicians about the possible occurrence of myocardial infarction or stroke. Here, human antibodies (HuAbs) selected by phage-display for their recognition of over-expressed biomarkers of the pathology are proposed as targeting ligands. They were further engineered for site-specific grafting, either by introducing Cysteine or Sortase recognition tags, and used to target contrast agents for MRI, fluorescence, or PET imaging. In vitro and ex vivo validation studies were carried out on atheroma sections of animal models. In vivo studies in the ApoE-/- mouse model were realized with the anti-platelet TEG4 HuAb using MRI, which provided insights on the biological relevance and feasibility to detect platelets-rich, high-risk atheroma plaques. The development of contrast agents useful in multi-modality imaging, and multi-functionalized with HuAbs is underway. It should serve as an accurate molecular imaging method for atherosclerosis, further more easily translated into the clinical arena
Patruno, Jolanda. "Polarimetric RADARSAT-2 and ALOS PALSAR multi-frequency analysis over the archaeological site of Gebel Barkal (Sudan)". Phd thesis, Université Rennes 1, 2014. http://tel.archives-ouvertes.fr/tel-01061287.
Pełny tekst źródłaSchumacher, Dominik [Verfasser], Christian P. R. [Gutachter] Hackenberger, Dorothea [Gutachter] Fiedler i Heinreich [Gutachter] Leonhardt. "Site-specific functionalization of antigen binding proteins for cellular delivery, imaging and target modulation / Dominik Schumacher ; Gutachter: Christian P. R. Hackenberger, Dorothea Fiedler, Heinreich Leonhardt". Berlin : Humboldt-Universität zu Berlin, 2017. http://d-nb.info/1185578390/34.
Pełny tekst źródłaAssaad, Jamal. "Modélisation des transducteurs piézoélectriques haute fréquence à l'aide de la méthode des éléments finis". Valenciennes, 1992. https://ged.uphf.fr/nuxeo/site/esupversions/daff1271-db25-4894-82dd-828d666c589c.
Pełny tekst źródłaParmentier, Alain. "Acquisition de cartes denses pour la génération et le contrôle de formes vestimentaires". Valenciennes, 1994. https://ged.uphf.fr/nuxeo/site/esupversions/96d52b8e-37f9-4146-8eaf-405f79a9426f.
Pełny tekst źródłaWilson, Louise Ann. "Emplacing, re-imaging and transforming 'missing' life-events : a feminine sublime approach to the creation of socially engaged scenography in site-specific walking-performance in rural landscapes". Thesis, Lancaster University, 2017. http://eprints.lancs.ac.uk/85071/.
Pełny tekst źródłaDebavelaere, Dorothée. "Caractérisation de progéniteurs osseux en culture, développement d'une technique ultrasonore d'analyse dynamique". Valenciennes, 1996. https://ged.uphf.fr/nuxeo/site/esupversions/155642e1-ddd5-4273-99c5-ea8f7bf9663f.
Pełny tekst źródłaDore, Nicole. "Polarimetric multi-incidence angle analysis over the archaeological site of Samarra by means of RADARSAT-2 and ALOS PALSAR satellites datasets". Phd thesis, Université Rennes 1, 2014. http://tel.archives-ouvertes.fr/tel-01060848.
Pełny tekst źródłaBerrou, Anne. "Subporphyrines et liposomes bimodaux ou théranostiques pour l’imagerie Cherenkov". Thesis, Bourgogne Franche-Comté, 2020. https://nuxeo.u-bourgogne.fr/nuxeo/site/esupversions/9533a12b-cbe8-4d29-ab6a-8e43c91995fe.
Pełny tekst źródłaThis work is relevant to Cherenkov Luminescence Imaging (CLI), an imaging approach that appeared in 2009 and relies on radionuclides that emit Cherenkov Radiation (CR).The study specifically developed molecular and nanoparticular tools aimed at addressing the association of CR with photodynamic therapy (PDT), optical imaging from fluorescence emission, and MRI.The molecular probes belong to the subporphyrin family, which are tripyrrolic porphyrin analogs, the advantages of which are four-fold : they absorb in an area of the electromagnetic spectrum that matches the CR maximum peak emission, their radiolabeling is potentially achievable, they are fairly stable and their concave shape prevents aggregation. The subporphyrin structure has been optimized to get them in the biological field, such as fluorophores in microscopy and photosensitizers in PDT. Subsequent study carried out in the presence of radiolabeled fluorodeoxyglucose [18F]-FDG and in the absence of exogenous light sources showed the relevance of using subporphyrins in PDT-Cherenkov.Nanoparticular probes are liposomes that embark two components in two dedicated sites among three available at the lipidic vesicle: outer surface, bilayer, lumen. Two couples of sites have been examined. The resulting simultaneous incorporation of hydrophilic and hydrophobic imaging agents allowed to achieve or envision the followings : a) biocompatibility of the latter (vectorization in aqueous media to skip the co-solvent addition (such as DMSO) in biological studies), b) simultaneous double cell labelling on two different sites (nucleus, cytoplasm), c) future liposomal CRET and CRET-FRET transfers, d) incorporation of MRI contrast agents and future radiolabelling leading to magneto-optical (nuclear) imaging agents are aimed at better addressing/narrowing the gap between both optical and magnetic imaging techniques
Delebarre, Christophe. "Caractérisation des milieux hétérogènes par traitement du signal ultrasonore rétrodiffusé". Valenciennes, 1987. https://ged.uphf.fr/nuxeo/site/esupversions/8a8ee375-5986-4f43-bd0c-148bf953452b.
Pełny tekst źródłaLin, Qiaojin. "Axonal translation and links to neuropathies". Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/273662.
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