Rozprawy doktorskie na temat „Human Biophysics”
Utwórz poprawne odniesienie w stylach APA, MLA, Chicago, Harvard i wielu innych
Sprawdź 50 najlepszych rozpraw doktorskich naukowych na temat „Human Biophysics”.
Przycisk „Dodaj do bibliografii” jest dostępny obok każdej pracy w bibliografii. Użyj go – a my automatycznie utworzymy odniesienie bibliograficzne do wybranej pracy w stylu cytowania, którego potrzebujesz: APA, MLA, Harvard, Chicago, Vancouver itp.
Możesz również pobrać pełny tekst publikacji naukowej w formacie „.pdf” i przeczytać adnotację do pracy online, jeśli odpowiednie parametry są dostępne w metadanych.
Przeglądaj rozprawy doktorskie z różnych dziedzin i twórz odpowiednie bibliografie.
Ogilvie, Julian Andrew. "Functional specialisation in human visual processing". Thesis, Imperial College London, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.286444.
Pełny tekst źródłaSchluter, Nathaniel D. "Human premotor cortex : imaging and interference". Thesis, University of Oxford, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.284344.
Pełny tekst źródłaWalker, Lloyd T. "The biomechanics of the human foot". Thesis, University of Strathclyde, 1991. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=21131.
Pełny tekst źródłaSchechtman, Helio. "Mechanical characterisation of fatigue failure in human tendons". Thesis, Queen Mary, University of London, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297451.
Pełny tekst źródłaAlexopoulos, Evangelos Demetrios. "Extracellular matrix associated with human luteinizing granulosa cells". Thesis, University of Southampton, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.369867.
Pełny tekst źródłaScase, Mark O. "Studies on normal and impaired human colour vision". Thesis, Keele University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.254775.
Pełny tekst źródłaTha, Susan P. L. "Interaction forces between human red cells aggutinated by antibody". Thesis, McGill University, 1987. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=75421.
Pełny tekst źródłaMicropipet aspiration was applied to the same red cell-antibody system. Separation forces were $ sim2{1 over2}$ fold greater than for normal forces of the traveling microtube technique. Non-uniformity of red cell adhesiveness was also demonstrated.
Moran, Carmel Mary. "The physical basis for ultrasonic investigation of human skin". Thesis, Institute of Cancer Research (University Of London), 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.246108.
Pełny tekst źródłaQuantock, Andrew James. "The stromal ultrastructure of normal and pathologic human corneas". Thesis, Open University, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293665.
Pełny tekst źródłaJones, Simon Richard. "Non-invasive analysis of human retinal and cortical visual function". Thesis, Imperial College London, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312379.
Pełny tekst źródłaMansfield, John Stephen. "The influence of spatial contrast processing on human stereoscopic vision". Thesis, University of Oxford, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.291325.
Pełny tekst źródłaBell, David N. "Physical factors governing the aggregation of human platelets in sheared suspensions". Thesis, McGill University, 1988. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=75873.
Pełny tekst źródłaPrince, Simon J. D. "Measurement and encoding of binocular disparity in the human visual system". Thesis, University of Oxford, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.299231.
Pełny tekst źródłaHo, Joseph Daniel. "Heterologous expression of human membrane protein drug targets and the X-ray crystallographic determination of the human aquaporin 4 structure". Diss., Search in ProQuest Dissertations & Theses. UC Only, 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3378489.
Pełny tekst źródłaRoberts, Stephen John. "Analysis of the human sleep electroencephalogram using a self-organising neural network". Thesis, University of Oxford, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302898.
Pełny tekst źródłaYu, Zhongbo. "Mechanochemistry of Human DNA G-quadruplexes Revealed by Single-molecule Optical Tweezers". Kent State University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=kent1369366966.
Pełny tekst źródłaMeadley, Stacey. "Investigation of the structure of healthy and diseased human ascending aorta by multiphoton microscopy". Thesis, McGill University, 2009. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=66789.
Pełny tekst źródłaPeu d'études portent sur la microstructure de l'aorte ascendante humaine et aucune relative à la composition biochimique et à la mécanique des tissus ont été réalisées. Cette étude utilise la microscopie multiphotonique, un nouvel outil employé en imagerie biologique, afin d'étudier la structure de deux protéines, l'élastine et le collagène, dans l'aorte ascendante. Des tissus en santé d'aortes ascendantes dilatées ont été examinés dans deux régions, la courbure intérieure et extérieure. Les aortes dilatées ont été classées par type de valve, bicuspide ou tricuspide. La morphologie des fenestrations dans les lames élastiques de l'aorte ainsi que les fibres de collagène ont été quantifiées. Les résultats démontrent une différence dans les divers types de valve aortique et également dans les différentes régions analysées. Les corrélations entre la microstructure et la composition biochimique ainsi que la mécanique des tissus suggèrent que la microscopie multiphotonique pourrait être utilisée pour la détermination rapide de la biochimie et la biomécanique de l'aorte ascendante humaine lors de chirurgies aortiques. Cette étude démontre que le remodelage de la paroi se produit simultanément avec la dilatation de l'aorte ascendante.
Breen, Alan Clark. "The measurement of the kinematics of the human spine using videofluoroscopy and image processing". Thesis, University of Southampton, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303090.
Pełny tekst źródłaAbbott, Heather Elizabeth. "Comparisons of the factors influencing intrinsic radiosensitivity between two isogenic human ovarian carcinoma cell lines". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0021/MQ57080.pdf.
Pełny tekst źródłaLevesque, Ives. "Quantitative magnetic resonance imaging of magnetization transfer and T2 relaxation in human white matter pathology". Thesis, McGill University, 2009. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=66751.
Pełny tekst źródłaL'objectif principal de cette thèse est la réconciliation de deux techniques quantitatives d'imagerie par résonance magnétique, en apparence difféerentes, utilisées pour la caractérisation de la susbtance blanche du cerveau humain en santé ou affectée par la maladie. Les techniques d'imagerie quantitative par transfert de magnétisation (QTM) et d'analyse de la relaxation T2 par de multiples composantes (QT2) proposent toutes deux des mesures in vivo de la quantitée de myéline, mais à l'aide de modèles fondamentalement différents. D'un côté, l'imagerie QTM sonde la composante macro-moléculaire des tissues à l'aide d'un modèle à deux réservoirs pour le transfert de magnétisation. De l'autre, l'imagerie QT2 sépare les signaux acqueux provenant de compartiments micro-anatomiques distincts. Plus spécifiquement, cet ouvrage cherche à mieux comprendre l'interdépendance des mesures de ces deux techniques dans le contexte pathologique de la sclérose en plaques (SEP), pour ensuite les appliquer à l' étude de lésions aigues de SEP. En premier lieu, des simulations ont été effectuées pour évaluer la sensibilité de chaque technique aux caractéristiques d'un modèle plus complet de la substance blanche, qui découle de résultats in vitro publiés et incorpore quatre réservoirs de magnétisation. Ensuite, la reproductibilité de chacune des techniques a été évaluée; de plus, quelques variations élémentaires des méthodes d'acquisition et d'analyse des données examinées. En dernier lieu, les deux techniques ont été utilisées in vivo afin de mesurer les changements dynamiques des lésions aigues de SEP, présentant un hyper-signal rehaussée par un agent de contraste. Les résultats des simulations démontrent d'un point de vue théorique la sensibilité et les limites de chacune de ces technique aux changements dans la substance blanche. Ces résultats apportent égalem
Cohalan, Claire. "Cerebral blood volume changes during human neuronal activation: a comparative study of VASO and VERVE". Thesis, McGill University, 2009. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=66871.
Pełny tekst źródłaDeux techniques visant à mesurer les changements de volume sanguin cérébral durant l'activité neuronale sont étudiées. La première, Vascular Space Occupancy (VASO), mesure l'augmentation de l'ensemble du sang en mesurant la baisse du signal provenant de la matière grise, dans une image où la magnétisation du sang est nulle. La deuxième, Venous Refocusing for Volume Estimation (VERVE), mesure en particulier l'augmentation du volume sanguin veineux en exploitant la dépendance du T2 du sang partiellement deoxygéné sur l'intervalle de refocalisation τ180. Avec une tâche à la fois motrice et visuelle, un (ΔCBV/CBVrepos)totale de 25,0 ± 13,9 % et un (ΔCBV/CBVrepos)¬veineux de 3,9 ± 1,6 % ont été mesurés par VASO et VERVE, respectivement. La méthode VASO est facile à instrumenter, et jouit d'un ratio contraste-bruit plus élevé que VERVE, mais plusieurs compartiments autres que la matière grise contribuent à son signal. Moins d'effets gênants contribuent au signal de VERVE, mais celui-ci souffre d'un taux de puissance déposé élevé, parfois atteignant les limites imposées par la Commission Fédérale des Communications. Le volume activé de VERVE correspond mieux que le volume activé de VASO au volume activé de BOLD. Ce fait, et celui que VERVE mesure spécifiquement le volume veineux, prônent l'utilisation de cette technique dans une analyse de la contribution du volume sanguin au signal BOLD.
Sheppard, Steven James. "Diagnosis from single photon emission tomography images of the human brain using artificial neural networks". Thesis, University of Warwick, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307348.
Pełny tekst źródłaRatnasinghe, Duminda D. "Unusual Structure of a Human Middle Repetitive DNA". Digital Commons @ East Tennessee State University, 1993. https://dc.etsu.edu/etd/2767.
Pełny tekst źródłaDumoulin, Serge O. "Motion mechanisms and cortical areas in human vision : psychophysics and fMRI". Thesis, McGill University, 2003. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=82860.
Pełny tekst źródłaUsing psychophysical methods, a directional anisotropy was found for second-order but not first-order motion in peripheral vision. This anisotropy is interpreted as a functional dissociation implicating the second-order mechanism in optic flow processing.
Identification of early visual cortical areas is a prerequisite to any functional assessment of these visual areas. To this aim a novel human brain mapping method has been developed which automatically segments early human retinotopic visual areas. Unlike previous methods this procedure does not depend on a cortical surface reconstruction and thereby greatly simplifies the analysis.
In a combined psychophysical and fMRI study, distinct cortical regions, in occipital and parietal lobes, were preferentially activated by either first- or second-order motion. These results provide evidence for the idea that first-order motion is computed in V1 and second-order motion in later occipital visual areas. In addition the results suggest a functional dissociation of the two kinds of motion beyond the occipital lobe consistent with a role for the second-order mechanism in optic flow analysis.
Taylor, David W. Jr. "Structural Basis for RNA Processing by Human Dicer". Thesis, Yale University, 2014. http://pqdtopen.proquest.com/#viewpdf?dispub=3578460.
Pełny tekst źródłaDicer plays a central role in RNA interference pathways by cleaving double-stranded RNAs (dsRNAs) to produce small regulatory RNAs. Human Dicer can process long double-stranded and hairpin precursor RNAs to yield short interfering RNAs (siRNAs) or microRNAs (miRNAs), respectively. In humans, Argonaute2 (AGO2) assembles with the guide RNA-generating enzyme Dicer and either the RNA-binding protein TRBP or PACT to form a RISC-loading complex (RLC), which is necessary for efficient transfer of nascent siRNAs from Dicer to AGO2. Here, I have used electron microscopy and single particle analysis of human Dicer-RNA complexes and the RLC to gain insight into the structural basis for human Dicer's substrate preference and RISC-loading. My studies show that Dicer traps pre-siRNAs in a non-productive conformation, while interactions of Dicer with pre-miRNAs and dsRNA binding proteins induce structural changes in the enzyme that enable productive substrate recognition in the central catalytic channel. The RLC Dicer's N-terminal DExH/D domain, located in a short base branch, interacts with TRBP, whereas its C-terminal catalytic domains in the main body are proximal to AGO2. A model generated by docking the available atomic structures of Dicer and Argonaute homologs into the RLC reconstruction suggests a mechanism for siRNA transfer from Dicer to AGO2.
Vera, Carlos D. "Functional Characterization of Disease-Causing Mutations in Human Myosin Heavy Chain Genes". Thesis, University of Colorado at Boulder, 2018. http://pqdtopen.proquest.com/#viewpdf?dispub=10981347.
Pełny tekst źródłaBiophysical and biochemical imbalance of mechanisms relevant to muscle function, can result in morphological changes to the tissue. While the purpose of activities involving exercise is to modify the shape and size of skeletal muscle, and the length of these muscles allows wide ranges of stiffness and stretch to be applied, cardiac tissue is not meant to change much. However, stressful extrinsic factors (poor diet, chemotherapy, etc) or intrinsic factors like inherited mutations in muscle functioning genes can result in a myopathy or a disease of the muscle. In fact, another biological process that requires much compliance of many molecules is embryogenesis. Although the timeline of an embryonic structure is limited, compared to an adult heart and muscle composition, continuous and coordinated movement is essential, but cumulative, prolonged disruptions can be harmful. At the core of muscle biology is the myosin molecule which is a motor protein that hydrolyzes ATP, binds to actin, and the spatial dynamics of its function (contraction-relaxation) alter the length of muscle. Myosin cyclically follows specific steps and undertakes well-defined structural conformations during these events, but mutations can alter the time and stability of any of these aspects. In this thesis I did a comprehensive analysis of the ATPase cycle parameters for both embryonic and cardiac myosin and studied the effects of specific associated or linked mutations have on function. The multiple mutations were in the interest of cataloging common features and defects to identify mechanistic patterns. In a collaborative effort I also used these wet-lab measurements to simulate the cycle using a working kinetic model for the myosin ATPase cycle. We have found distinct differences between three different myopathies that will be discussed in the following chapters.
Niese, Brandon A. "Fabrication of microfluidic devices to probe cell mechanical properties of MDA-MB-231 human breast cancer cells". Ohio University Honors Tutorial College / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1556626033175996.
Pełny tekst źródłaRoy, David Michael. "Gestural human-machine interaction using neural networks for people with severe speech and motor impairment due to cerebral palsy". Thesis, City University London, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307917.
Pełny tekst źródłaBeck, Jennifer 1968. "Effects of chest wall configuration and electrode positioning on human diaphragmatic EMG". Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=22536.
Pełny tekst źródłaFive normal male subjects performed static contractions of the diaphragm at seven predetermined chest wall configurations. The EMGdi was measured with an array of eight steel rings mounted on a catheter, forming seven sequential pairs of electrodes, with an interelectrode distance of 10 mm. EMGdi signal quality was evaluated by computer algorithms. The pair of electrodes whose EMGdi signals (and power spectrums) were the least influenced by the MEdist filter was assumed to be closest to the diaphragm.
The results of the study indicated (1) EMGdi power spectrums and their associated CF values were strongly affected by the position of the diaphragm with respect to the multiple array esophageal electrode. CF decreased by approximately 1 Hz per mm displacement away from the diaphragm. (2) By controlling for the MEdist filter, there was no relationship found between changes in chest wall configuration and CF values. These data demonstrate that changes in chest wall configuration, and hence diaphragm length, do not influence the CF values of the EMGdi, if the distance between the electrodes and the diaphragm and signal quality are controlled for.
Carter, Richard John. "Small-angle scattering studies of human growth hormone and its complexes with antibody fragments and X-ray crystallographic studies of chromatin". Thesis, Liverpool John Moores University, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.337899.
Pełny tekst źródłaOursler, Stephen Mark. "A proposed mechanical-metabolic model of the human red blood cell". Thesis, University of Maryland, College Park, 2014. http://pqdtopen.proquest.com/#viewpdf?dispub=1561025.
Pełny tekst źródłaThe theoretical modeling and computational simulation of human red blood cells is of interest to researchers for both academic and practical reasons. The red blood cell is one of the simplest in the body, yet its complex behaviors are not fully understood. The ability to perform accurate simulations of the cell will assist efforts to treat disorders of the cell. In this thesis, a computational model of a human red blood cell that combines preexisting mechanical and metabolic models is proposed. The mechanical model is a coarse-grained molecular dynamics model, while the metabolic model considers the set of chemical reactions as a system of first-order ordinary differential equations. The models are coupled via the connectivity of the cytoskeleton with a novel method. A simulation environment is developed in MATLAB® to evaluate the combined model. The combined model and the simulation environment are described in detail and illustrated in this thesis.
Seckler, James Malcolm. "The Structural Dynamics of Human Immunodeficiency Virus Type I Reverse Transcriptase". Case Western Reserve University School of Graduate Studies / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=case1298562809.
Pełny tekst źródłaSaqar, Wedad Ali. "Characterization of Small Conductance Calcium-Activated Potassium Channels in a Human Lens Epithelium Cell Line (B3)". Wright State University / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=wright1401279427.
Pełny tekst źródłaMercier, Jean-Francois. "New modelling tools for the human genome project: 1 A study of the Ogston regime for small analytes and 2 Models for solid phase DNA amplification". Thesis, University of Ottawa (Canada), 2004. http://hdl.handle.net/10393/29141.
Pełny tekst źródłaSankeralli, Marcel John. "Investigation of the chromatic postreceptoral detection mechanisms of human colour vision using noise masking in cone contrast space". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape11/PQDD_0020/NQ44571.pdf.
Pełny tekst źródłaBuzan, Jonathan R. "Quantifying Human Heat Stress in Working Environments, and Their Relationship to Atmospheric Dynamics, Due to Global Climate Change". Thesis, Purdue University, 2018. http://pqdtopen.proquest.com/#viewpdf?dispub=10809312.
Pełny tekst źródłaHeat stress is a global issue that crosses socioeconomic status. Heat stress leads to reduced worker capacity on seasonal scales, and weekly to sub-daily timescales, incapacitation, morbidity, and mortality. This dissertation focuses on 2 distinct parts: quantification methods of heat stress, and heat stress applications.
Quantification methods of heat stress: Chapters 1–3 focus on historical analysis of heat stress. Chapter 1 is a detailed assessment of previous work in heat stress—methods, history, and future research out- look. Chapter 2 focuses on the implementation and quantification of a battery of heat stress metrics within the global circulation model framework. The ultimate outcome is a Fortran module, the HumanIndexMod [1], that may be run independently on individual datasets, or used with the Community Earth System Model 1, Community Land Model Version 5 (released February 2018 w/HumanIndexMod). Chapter 3 is an analysis of a battery of heat stress metrics with the focus on showing their differences in global circulation models, and thermodynamic predictability and scalability.
Heat stress applications: Chapters 4 and 5 focus on applications for physical impact modeling and economic outcomes. Chapter 4 quantifies labor impacts from heat stress due to the covariance or temperature, humidity, and radiation. My predictions of labor productivity losses from heat stress are amenable to Integrated Assessment Modeling. Chapter 5 is a preliminary economic impacts analysis–a 1st order sensitivity perturbation study for labor impacts–which will guide a flagship application for the Purdue University Big Idea Project, GLASS: Global to Local Analysis of Systems Sustainability. My labor productivity losses from heat stress will become a boundary condition for a series of sensitivity assessments intended to inform the policy making process to help achieve the United Nations Sustainability Development Goals.
Mao, Suifang. "Motile cilia of human airway epithelia mediate noncanonical hedgehog signaling". Diss., University of Iowa, 2018. https://ir.uiowa.edu/etd/6195.
Pełny tekst źródłaTrikha, Saurabh. "Unraveling the Molecular Mechanisms of Human Amylin Binding, Turnover and Toxicity in Pancreatic Cells". Thesis, The George Washington University, 2013. http://pqdtopen.proquest.com/#viewpdf?dispub=3590360.
Pełny tekst źródłaIslet amyloid polypeptide or amylin is a recently discovered 37 amino acid residue signaling protein (hormone) that is produced and co-secreted along with insulin by pancreatic beta-cells. In late onset of diabetes, amylin readily aggregates forming protein deposits or plaques that are toxic to beta-cells, resulting in beta-cell apoptosis. Given the well-known role of cholesterol and lipids in etiology of diabetes, I explored whether these two essential PM components regulate amylin assembly and aggregation on artificial (synthetic) membranes. Using high resolution imaging and spectroscopic approaches, I demonstrated that amylin undergoes facilitated aggregation and conformational changes in the presence of membranes composed of anionic lipids such as phosphatidylserine (PS). The presence of cholesterol on the other hand inhibited lipid-induced aggregation of amylin in solution and on model planar membranes. However, the patho-physiological consequence of cholesterol-regulated amylin polymerization on membranes, and biochemical mechanisms that protect beta-cells from amylin toxicity are poorly understood. Hence, in my subsequent study, I reported that PM cholesterol plays a key role in molecular recognition, sorting and internalization of toxic amylin oligomers but not monomers in pancreatic rat insulinoma and human islet cells. Depletion of PM cholesterol or the disruption of the cytoskeleton network inhibited internalization of amylin oligomers, which in turn enhanced extracellular oligomer accumulation and potentiated amylin toxicity. In contrast to oligomers, amylin monomers followed clathrin-dependent endocytosis, which was not sensitive to cholesterol depletion. Our studies identified an actin-mediated and cholesterol-dependent mechanism for selective uptake and clearance of amylin oligomers, impairment of which greatly potentiated amylin toxicity.
However, the exact uptake mechanism and trafficking routes of these molecular forms and their significance for amylin toxicity are yet to be determined. Hence, in my further study, I observed that pancreatic cells employed different strategies to eliminate amylin's toxic and non-toxic molecular forms. My study also revealed that macropinocytosis serves a major cyto-protective role in these cells, by clearing of amylin molecular forms. The overreaching goal was to fully elucidate the internalization and trafficking pathways of human amylin monomers and toxic oligomers in pancreatic cells.
Zhu, Yueming. "Polychlorinated biphenyl (PCB)-induced oxidative stress mediates cytotoxicity in human breast and prostate epithelial cells". Diss., University of Iowa, 2011. https://ir.uiowa.edu/etd/3026.
Pełny tekst źródłaVittal, Rao Geeta. "Identifying Functional Epitopes In Site 1 Of Human Prolactin Hormone". The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1226014118.
Pełny tekst źródłaShikov, Sergei. "Structural Determinants for Heparin Binding in Human Coagulation Factor XI". Diss., Temple University Libraries, 2008. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/13460.
Pełny tekst źródłaPh.D.
Coagulation factor XI plays an important role in the consolidation phase of blood coagulation. Previous studies from our laboratory and others have demonstrated that zymogen factor XI (FXI) binds to heparin with moderate (KD~110 nM) affinity via residues (K252, K253 and K255) located in the apple 3 (A3) domain of the molecule. In contrast, the enzyme, factor XIa (FXIa), was shown to bind to heparin with significantly higher affinity (~1.5 nM by ELISA) via residues (K529, R530 and R532) within the catalytic domain (CD). The interaction between heparin and FXIa potentiates the inhibition of FXIa by protease nexin-2 by 10-fold. In addition, related polyanions heparin and dextran sulfate inhibit the catalytic activity of FXIa. The present study was designed to determine the relative contributions of positively charged residues as well as the dimeric structure of FXI in heparin binding. During this project, wtFXI, FXIR504A, FXIK505A, FXIR507A, FXIR529A, FXIR530A, FXIR532A, and FXIR586A have been expressed and purified. All mutants were homogenous and identical to wtFXI on SDS-PAGE, clotting assays and 1G5 monoclonal antibody binding studied by SPR. In addition, monomeric FXI C321S/K331A was expressed and purified. Utilizing an ELISA assay, no difference in the affinity for heparin between FXIa and FXI was found. Surface plasmon resonance (SPR) data collected for FXI clearly indicate a complex interaction which does not conform to a simple 1:1 Langmuir binding model making it difficult to obtain quantitative information. The complexity of FXI interactions with heparin is likely to arise from the multivalent nature of the binding, in which both protein and heparin have multiple binding sites. Two positively charged residues in the FXI catalytic domain, FXIR507A and FXIR532A, were found to be particularly important for interaction with heparin. The FXIR507A and FXIR532A mutants demonstrated ~ 65% and ~50% decreases respectively in total number of heparin binding sites based on ELISA. Also, the apparent dissociation constants for FXIR507A (KDapp ~13 nM) and FXIR532A (KDapp ~21 nM ) were 6 and 10-fold increased respectively compared with 2.1 nM for the wtFXI. Mutant FXIR586A also demonstrated a defect in affinity (KDapp ~ 13 nM) without an effect on the Bmax. The monomeric FXIC321S/R331A was also characterized for its ability to bind heparin compared with wtFXI. Surprisingly, the monomeric FXI displayed defective binding to heparin according to ELISA (KDapp ~ 30 nM) and SPR methods. Thus, the unique homodimeric structure of FXI in addition to the residues both in its catalytic and A3 domain chains are necessary for high-affinity heparin binding.
Temple University--Theses
Everett, Judith Helen. "Structure of monolithic human insulin at 1.8A resolution and development of computer software for application in biophysics". Thesis, Liverpool John Moores University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.290739.
Pełny tekst źródłaMohanraj, Lathika. "REGULATION OF MDM2 MEDIATED NFκB2 PATHWAY IN HUMAN LUNG CANCER". VCU Scholars Compass, 2008. http://scholarscompass.vcu.edu/etd/1641.
Pełny tekst źródłaNguỹên, Quang. "Characterization of proteolytic agents involved in the degradation of human articular cartilage proteoglycan during aging and arthritis". Thesis, McGill University, 1990. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=74620.
Pełny tekst źródłaVenius, Jonas. "Fluorescence spectroscopy and imaging studies of functionally different human heart tissues". Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2013. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2013~D_20130124_081732-30187.
Pełny tekst źródłaTvarkingą širdies darbą užtikrina širdies laidžioji sistema (ŠLS). Ją pažeidus sutrinka širdies darbas. Pažeidimo rizika atsiranda operacijos metu, kadangi ŠLS yra raumeninės kilmės audinys ir vizualiai neatskiriamas nuo aplinkinių audinių. ŠLS pažeidimo galima būtų išvengti, jei būtų žinomas tikslus ŠLS išsidėstymas arba egzistuotų ŠLS vaizdinimo metodika. Deja, bet atskirų ŠLS dalių tikslus išsidėstymas vis dar tikslinamas, o patologijų atvejais apskritai nėra žinomas. ŠLS vaizdinimo metodikos, tinkančios in vivo taikymams, taipogi nėra. Atlikus širdies audinių tyrimus nustoviąja fluorescencine spektroskopija nustatyti charakteringi intensyvumų skirtumai. Remiantis šiais skirtumais sukurta ŠLS atskyrimo metodika, paremta intensyvumų santykių skaičiavimu. Suskaičiuota vertė R = I(330)/I(380) yra skirtinga ŠLS, miokardui (MK) ir jungiamąjam audiniui (JA).Tokia metodika yra nejautri tyrimo sąlygoms ir gali būti naudojama ŠLS nustatymui. Ištyrus širdies audinius laikinės skyros spektroskopija nustatyta, kad ŠLS ir MK fluorescencijos gyvavimo trukmė bei santykinė sudėtis reikšmingai nesiskiria, tuo tarpu JA ir ŠLS tiek fluorescencijos gyvavimo trukmės, tiek santykinė komponentinė sudėtis yra skirtinga. Ištyrus širdies audinius konfokaliniu atspindžio mikroskopu nustatyta, jog dėl skirtingų šviesą atspindinčių komponentų bei skirtingo jų išsidėstymo galima identifikuoti MK, JA, Purkinje ląsteles ir ŠLS pluoštus. Atlikus tyrimus in vivo nustatyta, jog operacijos metu... [toliau žr. visą tekstą]
Adams, Bret. "Double-Strand Break Repair Mechanisms in Human Embryonic Stem Cells". VCU Scholars Compass, 2010. http://scholarscompass.vcu.edu/etd/114.
Pełny tekst źródłaLloyd, Kenneth P. "Understanding Human Erythrocyte Glucose Transporter (GLUT1) Mediated Glucose Transport Phenomena Through Structural Analysis". eScholarship@UMMS, 2018. https://escholarship.umassmed.edu/gsbs_diss/962.
Pełny tekst źródłaLiu, Sheng. "NMR studies of RNA binding domains of human lysyl aminoacyl tRNA synthetase". University of Cincinnati / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1353343207.
Pełny tekst źródłaAmunugama, Ravindra Bandara. "Insights into Regulation of Human RAD51 Nucleoprotein Filament Activity During Homologous Recombination". The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1321984760.
Pełny tekst źródłaPeramo, Antonio. "Physical studies of glycosaminoglycans in relation to the adhesion properties of human cancer cells". [Tampa, Fla.] : University of South Florida, 2005. http://purl.fcla.edu/usf/dc/et/SFE0001414.
Pełny tekst źródła