Gotowe bibliografie tematyczne / HSQC experiments

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Gotowa bibliografia na temat „HSQC experiments”

Autor: Grafiati

Data publikacji: 6 września 2023

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Artykuły w czasopismach na temat "HSQC experiments"

Krishnamurthy, V. V. "Sensitivity-Enhanced 3D HSQC-TOCSY Experiments". Journal of Magnetic Resonance, Series B 106, nr 2 (luty 1995): 170–77. http://dx.doi.org/10.1006/jmrb.1995.1028.

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Xia, Youlin, Kong Hung Sze, Ning Li, Pang Chui Shaw i Guang Zhu. "Protein Dynamics Measurements by 3D HNCO Based NMR Experiments". Spectroscopy 16, nr 1 (2002): 1–13. http://dx.doi.org/10.1155/2002/828353.

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Protein dynamics can be characterized by relaxation parameters obtained from traditional 2D HSQC based NMR experiments. This approach is hampered when applied to proteins with severe spectral overlap. In the present work, several novel 3D TROSY-HNCO and 3D HSQC-HNCO based NMR experiments were applied for measuring15NT1,T2and1H-15N NOE with improved spectral dispersion by introducing a third13C dimension. The number of phase cycling steps in these 3D pulse sequences was restricted to two in order to minimize the time required to perform the dynamics measurements. For a uniformly 100%15N, 100%13C, and 70%2H-labelled trichosanthin sample (~27 kDa, 1.0 mM) at 30°C, the sensitivity of 3D TROSY-HNCO based experiment was, on the average, enhanced by 72% compared to that of 3D HSQC-HNCO based experiments. However, the 3D HSQC-HNCO based experiments should be more effective for non-deuterated proteins with smaller molecular weights and seriously overlapped 2D HSQC spectra. Results from the 3D TROSY-HNCO and 3D HSQC-HNCO based experiments were in good agreement with those obtained from traditional 2D HSQC based experiments.

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Weitzel, Alexander, Claudia Samol, Peter J. Oefner i Wolfram Gronwald. "Robust Metabolite Quantification from J-Compensated 2D 1H-13C-HSQC Experiments". Metabolites 10, nr 11 (7.11.2020): 449. http://dx.doi.org/10.3390/metabo10110449.

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The spectral resolution of 2D 1H-13C heteronuclear single quantum coherence (1H-13C-HSQC) nuclear magnetic resonance (NMR) spectra facilitates both metabolite identification and quantification in nuclear magnetic resonance-based metabolomics. However, quantification is complicated by variations in magnetization transfer, which among others originate mainly from scalar coupling differences. Methods that compensate for variation in scalar coupling include the generation of calibration factors for individual signals or the use of additional pulse sequence schemes such as quantitative HSQC (Q-HSQC) that suppress the JCH-dependence by modulating the polarization transfer delays of HSQC or, additionally, employ a pure-shift homodecoupling approach in the 1H dimension, such as Quantitative, Perfected and Pure Shifted HSQC (QUIPU-HSQC). To test the quantitative accuracy of these three methods, employing a 600 MHz NMR spectrometer equipped with a helium cooled cryoprobe, a Latin-square design that covered the physiological concentration ranges of 10 metabolites was used. The results show the suitability of all three methods for the quantification of highly abundant metabolites. However, the substantially increased residual water signal observed in QUIPU-HSQC spectra impeded the quantification of low abundant metabolites located near the residual water signal, thus limiting its utility in high-throughput metabolite fingerprinting studies.

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Rouger, Laetitia, Serge Akoka i Patrick Giraudeau. "Optimized decoupling schemes in ultrafast HSQC experiments". Journal of Magnetic Resonance 283 (październik 2017): 89–95. http://dx.doi.org/10.1016/j.jmr.2017.08.015.

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Yang, Linyao, Jiangchao Du, Rongtao Li, Fei Yu i Jindong Zhong. "Bodiniosides S–Y, Seven New Triterpenoid Saponins from Elsholtzia bodinieri and Their Anti-Influenza Activities". Molecules 26, nr 21 (29.10.2021): 6535. http://dx.doi.org/10.3390/molecules26216535.

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Investigation of the n-BuOH extract of the aerial parts of Elsholtzia bodinieri led to the isolation of seven new triterpenoid saponins, Bodiniosides S–Y (1–7, resp.). Their strictures were elucidated on the basis of spectroscopic techniques, including HSQC, HSBC, and HSQC–TOCSY experiments, together with acid hydrolysis and GC analysis. The anti-influenza activities of compounds 1–7 were evaluated against A/WSN/33/2009 (H1N1) virus in MDCK cells. The results showed that compounds 2 and 5 exhibited moderate anti-influenza activities against A/WSN/33/2009 (H1N1), with inhibition rates of 35.33% and 24.08%, respectively.

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Kiraly, Peter, Mathias Nilsson i Gareth A. Morris. "Practical aspects of real-time pure shift HSQC experiments". Magnetic Resonance in Chemistry 56, nr 10 (12.01.2018): 993–1005. http://dx.doi.org/10.1002/mrc.4704.

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Gschwind, Ruth M., Xiulan Xie i Pattuparambil R. Rajamohanan. "Gs-HSQC-NOESY versus gs-NOESY-HSQC experiments: signal attenuation due to diffusion; application to symmetrical molecules". Magnetic Resonance in Chemistry 42, nr 3 (12.02.2004): 308–12. http://dx.doi.org/10.1002/mrc.1344.

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Hu, Haitao, i Krish Krishnamurthy. "Doubly compensated multiplicity-edited HSQC experiments utilizing broadband inversion pulses". Magnetic Resonance in Chemistry 46, nr 7 (2008): 683–89. http://dx.doi.org/10.1002/mrc.2221.

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Charlier, Cyril, Neil Cox, Sophie Martine Prud'homme, Alain Geffard, Jean-Marc Nuzillard, Burkhard Luy i Guy Lippens. "Virtual decoupling to break the simplification versus resolution trade-off in nuclear magnetic resonance of complex metabolic mixtures". Magnetic Resonance 2, nr 2 (10.08.2021): 619–27. http://dx.doi.org/10.5194/mr-2-619-2021.

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Abstract. The heteronuclear single quantum correlation (HSQC) experiment developed by Bodenhausen and Ruben (1980) in the early days of modern nuclear magnetic resonance (NMR) is without a doubt one of the most widely used experiments, with applications in almost every aspect of NMR including metabolomics. Acquiring this experiment, however, always implies a trade-off: simplification versus resolution. Here, we present a method that artificially lifts this barrier and demonstrate its application towards metabolite identification in a complex mixture. Based on the measurement of clean in-phase and clean anti-phase (CLIP/CLAP) HSQC spectra (Enthart et al., 2008), we construct a virtually decoupled HSQC (vd-HSQC) spectrum that maintains the highest possible resolution in the proton dimension. Combining this vd-HSQC spectrum with a J-resolved spectrum (Pell and Keeler, 2007) provides useful information for the one-dimensional proton spectrum assignment and for the identification of metabolites in Dreissena polymorpha (Prud'homme et al., 2020).

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Nolis, Pau, Kumar Motiram-Corral, Míriam Pérez-Trujillo i Teodor Parella. "Broadband homodecoupled time-shared 1H-13C and 1H-15N HSQC experiments". Journal of Magnetic Resonance 298 (styczeń 2019): 23–30. http://dx.doi.org/10.1016/j.jmr.2018.11.005.

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Rozprawy doktorskie na temat "HSQC experiments"

Castañar, Acedo Laura. "Development and application of modern pure shift NMR techniques and improved HSQC/HSQMBC experiments". Doctoral thesis, Universitat Autònoma de Barcelona, 2015. http://hdl.handle.net/10803/300751.

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La presente tesis doctoral está centrada en el diseño y aplicación de nuevas metodologías de Resonancia Magnética Nuclear (RMN), con especial interés en (i) la obtención de espectros de RMN de protón homonuclearmente desacoplados (denominados “pure shift”) y (ii) la determinación de las constantes de acoplamiento homo- y heteronucleares en medio isotrópico y anisotrópico a través de nuevos experimentos HSQC y HSQMBC. La tesis se presenta como compendio de artículos, los cuales han sido ya publicados en revistas científicas internacionales de reconocido prestigio, de modo que todos los resultados aquí expuestos han sido previamente evaluados y analizados por investigadores expertos en el campo de la RMN y de la química. Durante esta tesis doctoral se han diseñado una gran variedad de nuevos experimentos “pure shift”. La mayoría de ellos están basados en la técnica HOBS (Homodecoupled Band-Selective) mediante la cual es posible obtener espectros “pure shift” con una elevadísima resolución espectral manteniendo la máxima sensibilidad. El método HOBS ha sido incorporado en una gran variedad de experimentos 1D/2D convencionales los cuales han sido aplicados con éxito en la medida simple y precisa de tiempos de relajación T1 y T2 en áreas congestionadas, en la medida de contantes de acoplamiento heteronuclear a partir de multipletes simplificados, en la determinación de pequeñas diferencias de desplazamiento químico en estudios de enantiodiferenciación y en el análisis de mezclas complejas. Por otro lado, la medición y el uso práctico de las constantes de acoplamiento ha sido un importante tema de estudio en el campo de la RMN durante muchos años, pero todavía hay una serie de problemas sin resolver. Durante esta tesis doctoral se han desarrollado nuevos experimentos HSQC y HSQMBC, que permiten llevar a cabo la medida precisa y exacta de las constantes de acoplamientos a través del análisis simple y directo de los picos y sin la necesidad de utilizar complejos post-procesamientos.
The present doctoral thesis is framed within the Nuclear Magnetic Resonance (NMR) spectroscopy field, more specifically in the design of modern NMR methodologies. The research work carried out is focused on the design and application of new and modern NMR methodologies (i) to perform efficient broadband 1H homodecoupling in 1D/2D NMR experiments and (ii) to accurately determine homo- and heteronuclear coupling constants in isotropic and anisotropic conditions through improved HSQC and HSQMBC-type experiments. The thesis is presented as a compendium of publications, which have been published in prestigious peer-reviewed international scientific journals. Therefore, all the results here exposed have been previously evaluated by expert researchers in the fields of the NMR spectroscopy and chemistry. Several 1H homodecoupled NMR experiments have been developed along this doctoral thesis. Most of them are based on the Homodecoupled Band-Selective (HOBS) experiment through which it is possible to obtain homodecoupled NMR spectra with full sensitivity. HOBS methods offer a powerful and simple way to obtain high-resolved 1D and 2D NMR spectra, as efficiently demonstrated for the accurate, simple and direct measurement of T1/T2 relaxation times in overlapped regions, the determination of heteronuclear coupling constants from simplified multiplets, the determination of very small chemical shift differences with success application in enantiodifferentation studies or the analysis of highly complex mixtures. In addition, the concept of ultra-high-resolution NMR spectroscopy has been tested by combining several resolution-enhanced techniques into a single NMR experiment. On the other hand, for many years the measurement and practical use of coupling constants have been a timely topic but there are still a number of unresolved issues. Along this doctoral thesis a suite of robust HSQC and HSQMBC experiments have been developed which provide accurate and precise measurements of couplings constants through simple and direct analysis of cross-peaks without involving complex post-processing data manipulation.

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Plensa, Nebot Isabel. "Hiperplasia suprarrenal congénita por defecto en la enzima 21-hidroxilasa: caracterización por el sistema HLA y aportación de la biología molecular, La". Doctoral thesis, Universitat de Barcelona, 2003. http://hdl.handle.net/10803/2982.

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La hiperplasia suprarrenal congénita (HSC) comprende un conjunto de trastornos hereditarios de la esteroidogénesis, causados por la deficiencia de alguna de las enzimas necesarias para la conversión del colesterol en cortisol. El más frecuente es el déficit en 21-hidroxilasa, responsable de cerca del 90% de los casos de HSC. El déficit en 21-hidroxilasa es una enfermedad hereditaria autosómica recesiva, causado por una alteración genética en el gen CYP21B que codifica la enzima.

Esta enfermedad presenta un espectro muy amplio de manifestaciones clínicas; desde formas severas que se presentan al nacimiento, en las que la virilización puede ir o no acompañada de un síndrome pierde sal, hasta formas más leves en que los signos de virilización se presentan más tardíamente. La incidencia de las formas clásicas de este déficit, oscila alrededor de 1:10.000-15.000 nacimientos, mientras la de las formas no clásicas se ha estimado en 1:1.000 en la población blanca, con marcadas variaciones étnicas.

El objetivo ha sido la caracterización clínica, biológica y genética de la HSC por déficit en 21-hidroxilasa de una población de la Comunitat Autònoma de Catalunya, con el fin de poder realizar un diagnóstico etiológico concluyente y establecer el consejo genético familiar completo.

Han colaborado 53 familias (106 cromosomas no emparentados) con al menos un hijo diagnosticado de HSC por déficit en 21-hidroxilasa, siendo el total de individuos estudiados de 214. De este total, 71 son pacientes que según los síntomas clínicos fueron clasificados en: 13 SW; 5 SV y 53 NC; 102 son progenitores y 41 son hermanos de dichos pacientes, ninguno de ellos clínicamente afecto.

Para la determinación de la función hormonal se practicó una prueba de estimulación con corticotropina (test de ACTH), con valoración de las concentraciones plasmáticas de 17-hidroxiprogesterona antes y tras 30 minutos de la inyección. La determinación de los antígenos de Clase I y Clase II del Sistema HLA, se realizó mediante la técnica de Microlinfocitotoxicidad. El análisis genético consistió en la detección de grandes reordenamientos mediante la técnica de "Southern blot", y la detección de nueve de las mutaciones puntuales más comunes mediante el diseño de una nueva metodología en la que se emplean dos estrategias según la mutación a detectar: ganancia o pérdida de al menos una diana de restricción en el producto de PCR amplificado a partir de DNA genómico o creación de una diana de restricción por amplificación (ACRS-PCR) y posterior digestión con una enzima de restricción apropiada.

El test de ACTH se confirma como fiable, ya que no se observaron falsos positivos ni falsos negativos en los valores obtenidos de 17-hidroxiprogesterona post estimulación. El nomograma de 17-hidroxiprogesterona proporciona un patrón hormonal que facilita, en la mayoría de los casos, el diagnóstico de las distintas formas de presentación clínica del déficit. No se observó la presencia del antígeno HLA-Bw47 en ninguno de los grupos analizados. Las formas no clásicas se encuentran significativamente asociadas al haplotipo HLA-B14-DR1, mientras que las formas clásicas al antígeno HLA-B5(w51).

La metodología utilizada para el análisis molecular del gen ha permitido detectar la mutación causal en el 89,62% de los cromosomas afectos, evaluados globalmente, consolidándose ésta como altamente efectiva en el diagnóstico de esta patología. Las mutaciones más frecuentes observadas han sido: Intrón 2 (50%) en SW; Intrón 2 (50%) y I172N (16,6%) en SV y V281L (61,5%) en NC. El análisis de los pedigrees ha permitido diagnosticar veintiséis formas crípticas y revelar en dos casos la presencia de mutaciones originadas de novo. La concordancia hallada entre el genotipo y el fenotipo ha sido del 97,2%, de ahí, que las predicciones desde el genotipo han de ser hechas todavía con gran cautela.

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Veleba, Ondřej. "Studium vlivu složení na mechanické vlastnosti vysokohodnotného betonu". Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2008. http://www.nusl.cz/ntk/nusl-216217.

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This work is devoted to study the influence of the composition on mechanical properties of high performance concret based on portland cement. 29 samples of high performance concrete (HPC) warying in composition were prepared. The constituents used for HPC preparation were: cement Aalborg White, silica fume, finelly ground blast furnace slag, finelly ground silica, calcinated bauxite and polycarboxylate based superplasticizer. The mechanical parameters (flexural and compressive strength) of the samples were observed after 7 and 28 days of moist curing. Compressive strength values after 28 days were in the range of 92 to 194 MPa and the flexural strength values were in the range of 7 to 23 MPa (without using of fiber reinforcement). The graphs showing mechanical parameters depending on the mixture composition were constructed and consequently evaluated.

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Portolés, Flaj José Manuel. "Estudio experimental y numérico de soportes tubulares circulares de acero esbeltos rellenos de hormigón de alta resistencia". Doctoral thesis, Universitat Jaume I, 2010. http://hdl.handle.net/10803/669145.

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En los últimos años la utilización de perfiles tubulares de acero rellenos de hormigón (CFT) se ha incrementado debido a su excelente comportamiento frente al sismo, alta resistencia, alta ductilidad y la gran capacidad de absorción de energía. Aunque España y en particular la Comunidad Valenciana no son zonas de riesgo sísmico elevado, el uso de este tipo de pilares ofrece también otras ventajas, como por ejemplo el incremento en la velocidad del proceso de construcción, la posibilidad de estandarización de las uniones y lo que es muy importante, una mayor resistencia al fuego. Por otra parte, el uso del hormigón de alta resistencia (HSC) en la construcción de estructuras de hormigón se está generalizando gracias al abaratamiento de su tecnología. La utilización de este material presenta enormes ventajas, sobre todo en elementos sometidos a axiles de compresión importantes, como ocurre en el caso de soportes de edificación o de pilas de puente. Sin embargo, el Eurocódigo 4, con el que de diseñan estas estructuras mixtas, queda limitado a hormigones de hasta 60 MPa. Dada la mayor resistencia seccional de las columnas CFT rellenas de HSC, es posible reducir su sección, para un mismo nivel de carga. De esta forma la esbeltez y los efectos de segundo orden cobran más importancia. En esta tesis doctoral se estudia el comportamiento de las columnas de acero, esbeltas, de sección circular, rellenas de hormigón de alta resistencia. Para ello se ha desarrollado una campaña experimental que ha sido ampliada con modelos numéricos. Se ha comprobado la validez del Eurocódigo 4 y se ha realizado un estudio paramétrico de las variables que influyen en la respuesta de estas columnas.

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Części książek na temat "HSQC experiments"

Longin, Teresa. "Using the HSQC Experiment to Teach 2D NMR Spectroscopy in Physical Chemistry". W ACS Symposium Series, 175–89. Washington, DC: American Chemical Society, 2021. http://dx.doi.org/10.1021/bk-2021-1376.ch012.

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Nik, Sara, Joshua T. Weinreb i Teresa V. Bowman. "Developmental HSC Microenvironments: Lessons from Zebrafish". W Advances in Experimental Medicine and Biology, 33–53. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-69194-7_4.

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Bouzid, Haytham, Benferhat Rabia i Tahar Hassaine Daouadji. "Curvature Ductility of Confined HSC Columns". W Lecture Notes in Civil Engineering, 253–62. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-99-1748-8_21.

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AbstractTo avoid brittle collapse of reinforced concrete (RC) structures, RC elements such as beams, columns, and shear walls are invited to ensure a minimum level of ductility. In this paper, an analytical method for predicting the curvature ductility factor of confined RC columns is developed. The stress–strain model of confined concrete provided by Eurocode 2 is adopted, and the effective lateral confining pressure is calculated according to the Eurocode 8. The curvature ductility factor is defined by the ratio of ultimate to yield curvature. In this context, a new hypothesis is adopted to calculate the yield curvature while the ultimate curvature is calculated based on axial load and the mechanical ratios of tension, web, and compression reinforcement. The results showed that the developed method has an excellent performance compared to the experimental results collected from previous researches, where the mean value and the standard deviation of the ratios predicted to experimental factors are equal to 1.02 and 0.17, respectively. Moreover, the calculated coefficient of determination is very close to 1.

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Ratajczak, Mariusz Z., Marcin Wysoczynski, Ryan Reca, Wu Wan, Ewa K. Zuba-Surma, Magda Kucia i Janina Ratajczak. "A Pivotal Role of Activation of Complement Cascade (CC) in Mobilization of Hematopoietic Stem/Progenitor Cells (HSPC)". W Advances in Experimental Medicine and Biology, 45–58. New York, NY: Springer US, 2008. http://dx.doi.org/10.1007/978-0-387-78952-1_4.

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Nagasawa, Takashi. "The Chemokine CXCL12 and Regulation of Hsc and B Lymphocyte Development in the Bone Marrow Niche". W Advances in Experimental Medicine and Biology, 69–75. Boston, MA: Springer US, 2007. http://dx.doi.org/10.1007/978-0-387-72009-8_9.

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Guthrie, Roderick I. L., i Mihaiela Isac. "Computational Fluid Dynamics and Experimental Results for the Horizontal Single Belt Casting (HSBC) of Aluminum Alloy Strips". W PRICM, 2859–66. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2013. http://dx.doi.org/10.1002/9781118792148.ch354.

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Guthrie, Roderick I. L., i Mihaiela Isac. "Computational Fluid Dynamics and Experimental Results for the Horizontal Single Belt Casting (HSBC) of Aluminum Alloy Strips". W Proceedings of the 8th Pacific Rim International Congress on Advanced Materials and Processing, 2859–66. Cham: Springer International Publishing, 2013. http://dx.doi.org/10.1007/978-3-319-48764-9_354.

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Castañar, Laura, i Teodor Parella. "Recent Advances in Small Molecule NMR: Improved HSQC and HSQMBC Experiments". W Annual Reports on NMR Spectroscopy, 163–232. Elsevier, 2015. http://dx.doi.org/10.1016/bs.arnmr.2014.10.004.

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Streszczenia konferencji na temat "HSQC experiments"

Zhang, Changqing, Yeqinq Liu, Yue Liu, Qinghua Hu, Xinwang Liu i Pengfei Zhu. "FISH-MML: Fisher-HSIC Multi-View Metric Learning". W Twenty-Seventh International Joint Conference on Artificial Intelligence {IJCAI-18}. California: International Joint Conferences on Artificial Intelligence Organization, 2018. http://dx.doi.org/10.24963/ijcai.2018/424.

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This work presents a simple yet effective model for multi-view metric learning, which aims to improve the classification of data with multiple views, e.g., multiple modalities or multiple types of features. The intrinsic correlation, different views describing same set of instances, makes it possible and necessary to jointly learn multiple metrics of different views, accordingly, we propose a multi-view metric learning method based on Fisher discriminant analysis (FDA) and Hilbert-Schmidt Independence Criteria (HSIC), termed as Fisher-HSIC Multi-View Metric Learning (FISH-MML). In our approach, the class separability is enforced in the spirit of FDA within each single view, while the consistence among different views is enhanced based on HSIC. Accordingly, both intra-view class separability and inter-view correlation are well addressed in a unified framework. The learned metrics can improve multi-view classification, and experimental results on real-world datasets demonstrate the effectiveness of the proposed method.

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Wang, Xinguo, David Moulton i Mirjam Fürth. "The Development of a Planning Boat Model and Environmental Measurements for Free Running Model Tests". W SNAME 14th International Marine Design Conference. SNAME, 2022. http://dx.doi.org/10.5957/imdc-2022-266.

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For decades, the performance and efficiency of High Speed Craft (HSC) have been tested and evaluated in towing tanks; these tests are performed under ideal conditions. During towed testing the scaled model is often only free to move in heave and pitch and the effect of surge cannot be evaluated. Self propelled tests are rare meaning the drag, noise and turbulence of the thruster propellers are not considered. This paper presents a detailed step by step workflow of designing and manufacturing a self-propelled (HSC) to be tested in a calm water free running test in the Offshore Technology Research Center (OTRC) wave basin in Texas A&M University. The paper shows the hull parametric design, thruster and propeller selection and the data acquisition system design. The study includes a quantitative and qualitative comparison for the effect of the propeller hull interaction, results are compared with exciting towing tank data for the same hull geometry. An experimental straight forward motion step response is implemented to identify the vehicle speed transfer function for closed loop forward speed controller design. The paper also presents the use of computer vision applications in the experiment measurements such as the Visual-SLAM algorithm for the accurate 6-DOF HSC motion tracking and the use of the stereo-vision for studying the wave pattern downstream of the hull quantitatively and qualitatively.

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Wang, Xinguo, Omar Sallam, David Moulton, Jared Richardson, Rory Sunderland, Benjamin McKeig i Mirjam Furth. "High-Speed Craft Model Design, Data Acquisition and Environment Monitoring for Free-Running Tests". W SNAME Maritime Convention. SNAME, 2022. http://dx.doi.org/10.5957/smc-2022-085.

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The majority of experimental research conducted on the hydrodynamics of High-Speed Crafts (HSC) has been done in towing tanks. This presents a couple of issues; towing facilities are expensive, and the ideal conditions of the tank create a disparity between experiment and open water performance. Free-running models tested in open water can overcome some of these limitations. This paper presents the detailed design of a free-running model for open water experimental application. The model development includes hull construction, design and integration of a propulsion & steering system, data acquisition system (DAQ) design and testing, as well as environmental monitoring methods.

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Hamada, Ahmed A., Omar Khaled Sallam, Colton R. Bonnaure, Patricia Itzel Rodriguez, Jack Stason i Mirjam Furth. "Design of a Free Running High Speed Craft Model for Testing Off the Coast of Galveston". W SNAME 26th Offshore Symposium. SNAME, 2021. http://dx.doi.org/10.5957/tos-2021-20.

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High speed planing craft (HSC) are used in a wide variety of marine applications, such as search and rescue, defense and surveillance, recreation, and as support and supply vessels to the offshore industry. Their design, improvement, and optimization mainly depend on a deep understanding of the dynamic interaction between the vehicle and the surrounding environment. Traditionally, measuring the dynamic behavior and response of the HSC due to waves has been performed in towing tanks, where measurements are recorded under ideal conditions. To get a more realistic idea of the performance of HSC, we aim to perform free-running tests in the Gulf of Mexico at the Texas A&M Galveston campus. The aim of the free running tests is to measure the behavior of the model under real-life conditions, such as realistic incident waves, wind, current speeds, and variable water depth regions. Designing such an experiment far from lab facilities is especially challenging. In this paper, a detailed step-by-step HSC experiment design workflow is presented to tackle the open sea testing challenges. The paper presents a design of a free running model to be built during the spring of 2021, which includes the basic geometric parameters selection, stability study, drag force prediction, required propulsion system's power estimation, manufacturing techniques, and sensors' data acquisition system design.

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Li, Changyong, Zhaofei Chen, Xinxin Ding i Shunbo Zhao. "Experimental Study on Crack Width of Reinforced HSC-on-SFRFLC Superposed Beams". W 2015 International Conference on Architectural, Civil and Hydraulics Engineering. Paris, France: Atlantis Press, 2015. http://dx.doi.org/10.2991/icache-15.2015.3.

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Li, Hongxia, Charles C. Okaeme, Weilin Yang i TieJun Zhang. "Effect of Surface Wettability and Gas/Liquid Velocity Ratio on Microscale Two-Phase Flow Patterns". W ASME 2016 5th International Conference on Micro/Nanoscale Heat and Mass Transfer. American Society of Mechanical Engineers, 2016. http://dx.doi.org/10.1115/mnhmt2016-6383.

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Predicting and controlling the flow regime transition of multiphase fluids in microchannels is essential for various energy applications, such as flow boiling, de-emulsification and oil recovery processes. This in turn requires a better understanding of multiphase flow behaviors in microchannels with various channel surface wettability, fluid interfacial tension and flow rates. In this paper, experiments and Lattice Boltzmann method (LBM) simulations are carried out to study complicated multiphase flow at micro or meso scales. With the Shan-Chen multiphase LBM model, the flow pattern transitions of adiabatic two phase flow in a microchannel were investigated. The effects of surface wettability and liquid/gas velocity ratio on the flow regime transition were further studied. A series of two-phase flow experiments were conducted on a PDMS microfluidic device under different gas/oil velocity ratios. Under various surface wettability conditions, our simulation results agree well with the flow visualization experiments equipped with a high speed camera (HSC). Our finding shows that the cross-section meniscus curve width, corresponding to the shadow in the HSC photo, increases with decreasing contact angle, which was confirmed by the simulated liquid/gas distribution. Besides the influence of surface wettability, the role of gas/liquid velocity ratio on two-phase flow regime transition was discussed in detail. The proposed approach paves the way to probe complicated physics of multiphase flows in microporous media.

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Wang, Jing, Feng Tian, Xiao Wang, Hongchuan Yu, Chang Hong Liu i Liang Yang. "Multi-Component Nonnegative Matrix Factorization". W Twenty-Sixth International Joint Conference on Artificial Intelligence. California: International Joint Conferences on Artificial Intelligence Organization, 2017. http://dx.doi.org/10.24963/ijcai.2017/407.

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Real data are usually complex and contain various components. For example, face images have expressions and genders. Each component mainly reflects one aspect of data and provides information others do not have. Therefore, exploring the semantic information of multiple components as well as the diversity among them is of great benefit to understand data comprehensively and in-depth. However, this cannot be achieved by current nonnegative matrix factorization (NMF)-based methods, despite that NMF has shown remarkable competitiveness in learning parts-based representation of data. To overcome this limitation, we propose a novel multi-component nonnegative matrix factorization (MCNMF). Instead of seeking for only one representation of data, MCNMF learns multiple representations simultaneously, with the help of the Hilbert Schmidt Independence Criterion (HSIC) as a diversity term. HSIC explores the diverse information among the representations, where each representation corresponds to a component. By integrating the multiple representations, a more comprehensive representation is then established. A new iterative updating optimization scheme is derived to solve the objective function of MCNMF, along with its correctness and convergence guarantees. Extensive experimental results on real-world datasets have shown that MCNMF not only achieves more accurate performance over the state-of-the-arts using the aggregated representation, but also interprets data from different aspects with the multiple representations, which is beyond what current NMFs can offer.

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Oguchi, Noritake, Junichi Sakai, Hiroyuki Inoue i Shigeru Komukai. "Critical Hardness for HSC in Deposit Welding on Steel Line Pipes Under Cathodic Protection". W 2006 International Pipeline Conference. ASMEDC, 2006. http://dx.doi.org/10.1115/ipc2006-10227.

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Repairing corrosion-damaged areas of operational steel line pipes by deposit welding performed without stopping the gas flowing inside is a common practice. However, this repair method tends to considerably increase the hardness of the weld metal and the HAZ, and it poses the risk of HSC under cathodic protection. In this study, the HSC susceptibility of the welding area was examined using welded full-scale C-ring specimens. The specimens were prepared from X42, X60, and X65 steel line pipes, whose outer diameter and longitudinal length were 610mm and 100 to 110mm, respectively, and whose nominal thickness was 10.3 to 15.1mm Deposit welding was applied to the middle of each specimen’s outer surface, and then a bolt through the specimen was tightened until the welded part was subjected to yield stress. The welded surface of the welding and its surrounding area were exposed to a test solution whose pH was adjusted to 7, and a cathodic current of 20mA/cm2 or 4mA/cm2 was applied for 500hours. Cracks appeared in the weld metals on the C-ring specimens to which a cathodic current of 20mA/cm2 had been applied. However, the propagation of the cracks ceased at the boundaries between the weld metal and the HAZ, and no cracks initiated in the HAZ. The maximum hardness of the HAZ was about 300 to 400Hv. These levels of hardness are far greater than 260Hv, which is the value obtained from experiments carried out using slow strain rate testing and is widely used in Japan as a criterion of hardness for avoiding HSC in the HAZ.

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KOWALSKI, D. "Nonlinear Analysis of Beams made of High Strength Concrete Prestressed with Unbonded Tendons". W Terotechnology XII. Materials Research Forum LLC, 2022. http://dx.doi.org/10.21741/9781644902059-12.

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Abstract. The paper presents numerical analysis methods of High-Strength Concrete (HSC) beams prestressed with unbonded tendons. Furthermore, it compares obtained results with experimental data from the literature. Prestressing tendons have been modelled in a discrete form, using one-dimensional finite elements. A temperature drop inflicted prestress force. Contact issues have been considered, i.e. friction and pressure at the interface between the cable and the duct wall. In the work, it was found that it is possible to obtain satisfactory accuracy of results with the model in use. Accurate P-Δ (load-deflection) curves were achieved matching experimental data.

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Santos, Erick de Matos, Thayse Batista Moreira, Ana Luiza de Freitas Magalhães Gomes, Ana Paula Alvares Ramos, Fábio Ribeiro Queiroz, Paulo Guilherme de Oliveira Salles i Letícia Conceição Braga. "STANDARDIZATION OF THE FICOLL GRADIENT TECHNIQUE FOR THE ISOLATION OF MONONUCLEAR CELLS FROM PERIPHERAL BLOOD". W Brazilian Breast Cancer Symposium 2022. Mastology, 2022. http://dx.doi.org/10.29289/259453942022v32s2030.

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Objective: The objective of this study was to standardize the Ficoll gradient technique for the circulating hematopoietic stem cell (HSC) isolation for the assembly of the peripheral blood mononuclear cell (PBMC) biorepository of breast cancer (BC) patients attended in the Clinical Oncology Service of Instituto Mário Penna. Methods: The study protocol was approved by the Ethics Committee of Instituto Mário Penna (CAEE 82703418.8.0000.5121). In recommended protocols, 15 mL of blood was used. At first, we adapted this volume due to the limited amounts of samples for research available. Blood was collected in a 9-mL sodium heparin tube. The experiments were performed in 50-mL conical tubes, but with reduced blood volume, and no PBMC ring was formed. It was necessary to change to 15 mL conical tubes. Finally, the remaining red blood cells were lysed with ammonium chloride. However, with the reduced volume, this solution lysed the PBMC too. Then, we decided to remove this step from the protocol. Results: We obtained 8.06×106 cells/mm3 with 80% viability. Data were confirmed by a Neubauer camera and an automatic cell counter. The HSCs were labeled with antibodies against CD34 and CD133 by flow cytometry. Conclusion: The characterization of HSCs is important to link tumor-associated HSCs with malignant and immunosuppressive phenotypes. Studies are in progress with this standardization, and they will permit us to perform the HSC characterization of BC patients with a better knowledge of tumor microenvironment.

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