Rozprawy doktorskie na temat „Glycolysis”
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Graham, James William Alexander. "Mitochondrial glycolysis in plants". Thesis, University of Oxford, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.445769.
Pełny tekst źródłaHale, R. D. "Glycolysis in Crithidia fasciculata". Thesis, University of Liverpool, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.234866.
Pełny tekst źródłaRobinson, Andrew James Cave. "Pyruvate kinase & glycolysis in potato". Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335799.
Pełny tekst źródłaPearce, Amanda K. "Regulation of glycolysis in Saccharomyces cerevisiae". Thesis, University of Aberdeen, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301297.
Pełny tekst źródłaTandon, Preeti. "S6K1 mediates oncogenic glycolysis in Pten deficient leukemia". University of Cincinnati / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1320682200.
Pełny tekst źródłaXintaropoulou, Chrysi. "Targeting aerobic glycolysis in breast and ovarian cancer". Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/29525.
Pełny tekst źródłaStefansson, G. "Effects of gases on post-mortem glycolysis in meats". Thesis, University of Leeds, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.371847.
Pełny tekst źródłaLong, Heidi Sarah. "Glycolysis and the control of pulmonary tone during hypoxia". Thesis, King's College London (University of London), 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.250174.
Pełny tekst źródłaShen, Qingwu. "AMP-activated protein kinase, postmortem glycolysis, and PSE meat". Laramie, Wyo. : University of Wyoming, 2007. http://proquest.umi.com/pqdweb?did=1338871331&sid=1&Fmt=2&clientId=18949&RQT=309&VName=PQD.
Pełny tekst źródłaNairn, Jacqueline. "Phosphoglycerate mutases from microorganisms". Thesis, University of Stirling, 1992. http://hdl.handle.net/1893/22851.
Pełny tekst źródłaRhoades, Ryan D. "Postmortem regulation of glycolysis by 6-phosphofructokinase in bovine muscle". Texas A&M University, 2004. http://hdl.handle.net/1969.1/1204.
Pełny tekst źródłaFiske, Brian Prescott. "Coordinated regulation of glycolysis and the folate one-carbon cycle". Thesis, Massachusetts Institute of Technology, 2015. http://hdl.handle.net/1721.1/98628.
Pełny tekst źródłaCataloged from PDF version of thesis.
Includes bibliographical references.
Rapid cell proliferation is characteristic of many biological systems, including cancer, normal development, and immune responses. At a basic level, proliferation requires that a cell synthesize a new copy of itself, with cell metabolism supplying the building blocks for new proteins, nucleic acids and lipids. Cancer and other proliferating cell types exhibit "aerobic glycolysis" characterized by elevated glucose uptake and conversion of glucose to lactate even in the presence of oxygen. Aerobic glycolysis is associated with anabolic reactions to generate new cellular material, but how aerobic glycolysis supports proliferative metabolism is not well understood. Pyruvate kinase (PK) catalyzes the last step in glycolysis, and while there is no evidence that PK activity is limiting for glycolysis, all proliferating cells express the PKM2 isoform of PK that is unique in having regulated activity which is decreased in the context of cellular proliferation. The simultaneous requirement for increased aerobic glycolysis and expression of the PKM2 isoform that is inhibited by growth signaling is a paradox, and it is unclear how elevated glucose metabolism enables rapid proliferation yet also requires decreased PKM2 activity. This thesis will explore two potential explanations for this paradox. The first hypothesizes the existence of an undiscovered enzyme that catalyzes a PK-like reaction, resolving the paradox by aligning increased flux through glycolysis with increased or unchanged activity of the PK step. The second hypothesis explores how PKM2 expression and PK inhibition supports proliferation by increasing serine synthesis from upstream glycolytic intermediates. This diverts one-carbon units into the folate pool to generate nucleotides via phosphoserine inhibition of SHMT1-mediated serine synthesis and one-carbon "wasting" in some cancer cells. We will also consider the reciprocal question of how folate one-carbon pool status in turn may regulate both PKM2 activity and glycolytic serine biosynthesis. The ability of PKM2 regulation to control folate metabolism for nucleotide synthesis explains for the first time at a mechanistic level one way that aerobic glycolysis promotes proliferative metabolism.
by Brian Prescott Fiske.
Ph. D.
Du, Preez Franco B. "Comparative cross-species analysis of detailed kinetic models of glycolysis". Thesis, Stellenbosch : University of Stellenbosch, 2009. http://hdl.handle.net/10019.1/1208.
Pełny tekst źródłaENGLISH ABSTRACT: With the recent advances in the field of molecular biology, there is an increased need to integrate data on the various constituents of the cell in kinetic models that can predict and describe cellular behavior. When working towards a description of the entire cell using such kinetic models, the question arises: How do we compare different models for a given biological network? This is the central question addressed in my thesis and I developed and applied mathematical and computational methods for comparing dozens of existing models of erythrocyte and yeast glycolysis. To compare the steady-state behavior in models of erythrocyte glycolysis, I focussed on the function of the pathway, which is to supply the cell with Gibbs-free energy (γ- phosphate of ATP). I used supply-demand analysis in the framework of metabolic control analysis to make this comparison, which revealed that the ATP concentrations were homeostatically buffered at varying supply rates. I also applied this approach to compare steady-state behavior in models of yeast glycolysis, finding that they were not necessarily optimized for homeostatic maintenance of the ATP concentration and that in models for this organism the rate of ATP production is often determined by the supply reactions of glycolysis. In addition, I tested whether a kinetic model can describe novel behavior if it is adjusted to conditions different from those for which the model was originally constructed. More specifically, using a model of steady-state yeast glycolysis, I showed that small adjustments to the original enzyme concentrations are enough to obtain an oscillating model, which shows a remarkable resemblance to the experimentally observed oscillations. Importantly, some of these enzyme concentrations changes are known to occur during the pre-treatment of the cells which is necessary to obtain oscillatory behavior. To the best of my knowledge, the resulting model is the first detailed kinetic model that describes the experimentally observed strong synchronization of glycolytic oscillations in yeast populations. To analyze the dynamic behavior of yeast glycolytic models and to compare different models in terms of dynamics, I introduced a framework used in physics and engineering to create a vector based, two dimensional graphical representation of the oscillating metabolites and reactions of glycolysis. Not only was it possible to make a concise comparison of the set of models, but with the method I could also quantify the contribution of the interactions in the network to the transduction of the oscillations. Furthermore I could distinguish between different mechanisms of oscillation for each of the models, and demonstrated how the framework can be used to create such representations for experimental data sets.
AFRIKAANSE OPSOMMING: Met die onlangse vooruitgang in die veld van molekulere biologie, is daar ?n toenemende behoefte om data rakende die verskeie komponente van die sel in kinetiese modelle te integreer, om sodanig selgedrag te voorspel en te beskryf. As daar gepoog word om ’n beskrywing van die sel as geheel te verkry d.m.v. sulke kinetiese modelle, onstaan die vraag: Hoe vergelyk ons verskillende modelle van ’n gegewe biologiese netwerk? Dit is die sentrale vraag wat my tesis aanspreek en ek het wiskundige en numeriese metodes ontwikkel en toegepas om talle bestaande modelle van gis- en eritrosietglikolise te vergelyk. Om die bestendige-toestand gedrag in modelle van eritrosietglikolise te vergelyk, het ek gefokus op die funksie van die padweg, naamlik om die sel met Gibbs-vrye energie (γ-fosfaat van ATP) te voorsien. Ek het vraag-aanbod analiese in die raamwerk van metaboliese kontrole analiese gebruik om hierdie vergelyking te maak, wat getoon het dat die ATP konsentrasies homeostaties gebuffer was by verskillende aanbod tempos. Ek het ook hierdie aanpak gebruik om die bestendige-toestand gedrag in modelle van gisglikolise te vergelyk, en het bevind dat hulle nie noodwendig geoptimiseer is om ?n homeostatiese balans in die ATP konsentrasie te handhaaf nie, en dat in modelle vir hierdie organisme, die tempo van ATP produksie dikwels bepaal word deur die aanbod reaksies van glikoliese. Ek het verder ook bepaal of so ?n kinetiese model nuwe soorte gedrag kan beskryf, as dit aangepas word aan omstandighede wat verskil van dié waarvoor die model oorspronklik gekonstrueer was. Meer spesifiek, deur ?n model van bestendige-toestand gisglikolise te gebruik, kon ek wys dat klein veranderinge aan die oorspronkline ensiem konsentrasies genoeg was om ?n ossilerende model te verkry, wat opmerklik ooreenstem met die eksperimenteel waargenome ossilasies. Let ook daarop dat sommige van hierdie ensiem konsentrasie veranderinge plaasvind tydens die voorafbehandeling van die selle, wat essensieel is om die ossilasies waar te neem. Tot die beste van my kennis is die model wat ek met hierdie prosedures verkry het, die eerste gedetaileerde kinetiese model wat die eksperimenteel waargenome sterk sinkronisasie in ossilerende gis populasies voorspel. Om gis glikolitiese modelle te vergelyk in terme van hul dinamiese gedrag, het ek ?n raamwerk wat in fisika en ingeneurswese gebruik word, ingespan om ?n vektor-gebasseerde, twee dimensionele grafiese voorstelling van die ossilerende metaboliete en reaksies te maak. Hierdie raamwerk het dit nie net moontlik gemaak om ?n kompakte vergelyking van ?n stel modelle te maak nie, maar ek kon ook die bydrae van interaksies in die netwerk tot transduksie van die ossilasies kwantifiseer. Ek kon verder onderskeid tref tussen die verskillende ossilasiemeganismes vir elk van die modelle, en het ook gedemonstreer hoe die raamwerk gebruik kan word om sulke voorstellings vir eksperimentele datastelle te skep.
Pasupuleti, Vinay. "Role of Glycolysis and Respiration in Sperm Metabolism and Motility". Kent State University / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=kent1195536178.
Pełny tekst źródłaKhatri, Shikha. "FOXO3a Regulates Glycolysis via Transcriptional Control of Tumor Suppressor TSC1". University of Cincinnati / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1282570293.
Pełny tekst źródłaABDALI, ANAHITA. "INHIBITION OF ANGIOGENESIS USING GLYCOLYSIS INHIBITORS: AN IN VITRO STUDY". Doctoral thesis, Università degli Studi di Milano, 2019. http://hdl.handle.net/2434/640647.
Pełny tekst źródłaDoyen, Jérôme. "Rôle des protéines de régulation du pH intracellulaire et du métabolisme énergétique dans les carcinomes du sein triple négatif". Thesis, Nice, 2013. http://www.theses.fr/2013NICE4147/document.
Pełny tekst źródłaAgressive cancers often harbor an exacerbated glycolytic metabolism with overexpression of proteins that maintain intracellular pH by extruding metabolic acid waste (via CAIX, CAXII, MCT1 and MCT4 among others). The "triple-negative" breast cancers (with no expression of estrogen, progesteron and Her-2 receptors) have an increased consumption of glucose and worse prognosis in comparison with other breast cancers. Immunohistochemical analysis of glycolytic proteins among 159 patients with TNEG breast cancer, showed that MCT4 was predictive for metastasis and death occurence. In vitro targeting of MCT4 by Zinc Finger Nuclease (ZFN) technique demonstrated an anti-proliferative effect. However, the maximal anti-proliferative effect was observed with the combination of MCT4/MCT1 (by pharmacological inhibition) and mitochondrial respiration by phenformine in the Hs578t TNEG cell line. This study demonstrated that targeting glycolytic protein could have a therapeutic effect in TNEG breast cancers. Another study also use targeting of glycolytic protein such as CAIX and CAXII to increase in vitro and in vivo radiosensitivity of colorectal cell lines while demonstrating an original mechanism of radiosensitization by increasing intracellular acidosis. Finally we demonstrated that the hypoxamiR miR210 was involved in the radioresistance of lung cancer cell line with a stronger impact than the oxygen effect
Keon, Claudia Anne. "Myocardial energy transduction in the isolated working rat heart". Thesis, University of Oxford, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244563.
Pełny tekst źródłaRayner, Mark. "Purification anad characterisation of pig heart 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase". Thesis, University of Liverpool, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.261842.
Pełny tekst źródłaYang, Min. "Synthesis of 5 thioglucose derivatives in the carbohydrate metabolic pathways in lactic acid bacteria". Thesis, University of Huddersfield, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274227.
Pełny tekst źródłaHernandez, Mark J. "Caveolin-1 A scaffold for microcompartmental organization of membrane-associated glycolysis". Diss., Columbia, Mo. : University of Missouri-Columbia, 2007. http://hdl.handle.net/10355/6007.
Pełny tekst źródłaThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Vita. "August 2007" Includes bibliographical references.
Anderson, Roxette Dianne. "Improving breast cancer therapy through oestrone analogue and glycolysis inhibitor synergism". Diss., University of Pretoria, 2017. http://hdl.handle.net/2263/63050.
Pełny tekst źródłaDissertation (MSc)--University of Pretoria, 2017.
Pharmacology
MSc
Unrestricted
Yuan, Fang. "DIFFERENTIAL EFFECTS OF GROWTH FACTORS ON GLYCOLYSIS IN OVARIAN CANCER CELLS". VCU Scholars Compass, 2013. http://scholarscompass.vcu.edu/etd/466.
Pełny tekst źródłaBorges, Figueiredo Ana Leonor. "Control of cell specification and migration during early frog development by PFKFB4, a key glycolysis regulator". Thesis, Paris 11, 2015. http://www.theses.fr/2015PA112107.
Pełny tekst źródłaEmbryonic ectoderm becomes specified into non-neural ectoderm, neural plate and neural border at the end of gastrulation. Neural border cells are the progenitors of the neural crest and placodes. The neural crest is a transient population of multipotent cells, which forms during neurulation. As the neural border elevates to form the neural tube, neural crest cells undergo an epithelial to mesenchymal transition, migrate extensively into the whole body to reach their final destinations and differentiate. Neural crest gives rise to multiple derivatives such as neurons and glia, facial cartilage, bones, melanocytes and sympatho-adrenal cells. A complex interplay of signaling and transcriptional regulations orchestrates these early patterning events. However, the first steps leading to NC formation and early specification at the NB are less understood. We analysed the NC transcriptome of frog embryos, to look for novel regulators of the early steps of NC formation. We found that the well-known glycolysis regulator PFKFB4, is expressed in early gastrula dorsal ectoderm, and in neurula neural crest cells. Here, we demonstrate that PFKFB4 regulates ectoderm specification via Akt signaling independently of glycolysis, thus demonstrating the first non-glycolytic function of PFKFB enzymes. Moreover, this regulation is essential to allow ectoderm embryonic progenitors to be patterned into neural plate, neural crest, placodes and definitive ectoderm, highlighting a novel developmental checkpoint. Moreover, we also demonstrate that PFKFB4 regulates later steps of neural crest formation. Our work highlights that regulators of cell metabolism accumulate non-metabolic related functions to control developmental steps during embryonic development
Benton, Geoffrey Marsing. "Aerobic glycolysis: A novel signature of premalignancy in disease-free breast tissue". Diss., Search in ProQuest Dissertations & Theses. UC Only. Search in ProQuest Dissertations & Theses. UC Only, 2010. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3390032.
Pełny tekst źródłaNowicki, Matthew William. "Development of lead compoundsfor trypanocidal drugs based on inhibitors of parasite glycolysis". Thesis, University of Edinburgh, 2005. http://hdl.handle.net/1842/15540.
Pełny tekst źródłaKelly, Annemarie. "The developmental potential of embryos and cells that are deficient in glycolysis". Thesis, University of Edinburgh, 1996. http://hdl.handle.net/1842/20602.
Pełny tekst źródłaAlbers, Renee Elizabeth. "Glycolytic Metabolism and Pregnancy Parameters in the Murine Placenta". Wright State University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=wright1513781057460423.
Pełny tekst źródłaSaeedi, Ramesh. "AMP-activated protein kinase and hypertrophic remodeling of heart muscle cells". Thesis, University of British Columbia, 2009. http://hdl.handle.net/2429/4065.
Pełny tekst źródłaRypniewski, W. R. "The structure of unliganded E. coli phosphofructokinase". Thesis, University of Cambridge, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233316.
Pełny tekst źródłaDancer, Jane Elizabeth. "The role of pyrophosphate:fructose 6-phosphate-1-phosphotransferase in plants". Thesis, University of Cambridge, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.330176.
Pełny tekst źródłaElgin, Jennifer May. "Determining the Underlying Factors of Fresh Ham Color Variation". Thesis, Virginia Tech, 2019. http://hdl.handle.net/10919/91406.
Pełny tekst źródłaMaster of Science
Baltan, Selva. "Long-term potentiation induced by temporary block of glycolysis in CA1 hippocampal neurons". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0016/NQ44609.pdf.
Pełny tekst źródłaCrimmins, Kay. "The significance of genetic regulation in the control of glycolysis in Saccharomyces cerevisiae". Thesis, University of Aberdeen, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320258.
Pełny tekst źródłaEicher, Johann Josef. "Understanding glycolysis in Escherichia coli : a systems approach using nuclear magnetic resonance spectroscopy". Thesis, Stellenbosch : Stellenbosch University, 2013. http://hdl.handle.net/10019.1/85730.
Pełny tekst źródłaENGLISH ABSTRACT: This dissertation explores the behaviour and regulation of central carbon metabolism in Escherichia coli K12 W3110 under fermentative microaerobic conditions. To achieve this, an integrative systems modelling approach was adopted, which is introduced in Chapter 1 along with a review of metabolism in E. coli. An open-source software suite NMRPy, developed using the Python programming language, is presented in Chapter 2. NMRPy provides a host functions for basic processing, analysis and visualisation of Nuclear Magnetic Resonance (NMR) spectroscopy data. In addition to this, NMRPy offers specialised functions for the deconvolution of arrayed reaction time series, which proved indispensable to the research presented in this dissertation. NMRPy presents an easy to use, extensible tool for both routine and advanced use. In Chapter 3, a novel methodology is presented which was developed for the effective and comprehensive determination of enzyme kinetic parameters for systems biology using NMR. In contrast to traditional enzyme kinetic assay methods, this new methodology is less labour-intensive and yields significantly more information per experiment. By fitting kinetic equations to real time NMR data, dynamic changes in substrates, products and allosteric modifiers are quantified and allowed to inform the parameter fitting procedure. These data contain information on cooperative substrate binding, reversibility, product inhibition and allosteric effects. The proposed methodology is applied to the study of the first two enzymes of the glycolytic pathway. In Chapter 4, the construction, parameterisation and validation of a number of kinetic models of glycolysis in E. coli under microaerobic conditions is detailed. To model the lower half of glycolysis, a similar technique was adopted as in Chapter 3, in which models representing the reactions from triosephosphate isomerase to pyruvate kinase were parameterised by fitting them to a collection of 31P NMR reaction time series. This approach extends the methodology to enzyme sub-networks, yielding data that encompass the full complexity of the network regulatory interactions. The verified kinetic models were subjected to scrutiny, the results of which are presented in Chapter 5. The value of the modelling approach is demonstrated by the ease with which cumbersome in vivo experiments can be performed in silico. A structural analysis of the model topology was conducted, elucidating the elementary flux modes of fermentative glycolysis in E. coli, and identifying a futile cycle around PEP carboxylase and PEP carboxykinase. Model steady-state behaviour and control properties were explored in silico under various degrees of ATP demand and oxygen availability and a number of hypotheses are presented, explaining the regulation of free energy in E. coli, and the metabolic responses of E. coli to changing redox demands. Amongst other things, the results demonstrated that the glucose importing phosphoenolpyruvate: phosphotransferase pathway controlled glycolytic flux, and that under microaerobic conditions E. coli is able to regulate redox balance not only by balancing flux between acetate and ethanol, but also by altering the balance of flux between acetate and lactate at the pyruvate formate lyase/lactate dehydrogenase branch point. This study demonstrates the value of an integrated computational and experimental systems approach to exploring biological phenomena.
AFRIKAANSE OPSOMMING: In hierdie proefskrif word die gedrag en regulering van die sentrale koolstofmetabolisme in Escherichia coli K12 W3110 onder fermenterende mikro-a¨erobiese toestande ondersoek. Dit is moontlik gemaak deur ’n ge¨ıntegreerde stelsel-modelleringsbenadering, wat in Hoofstuk 1 bekendgestel word. D´ıe hoofstuk verskaf ook ’n oorsig van die metabolisme in E. coli. ’n Oopbron-kodepakket NMRPy, wat in die programmeringstaal Python ontwikkel is, word in Hoofstuk 2 beskryf. NMRPy verskaf ’n aantal funksies vir die basiese verwerking, analise en visualisering van Kern-Magnetiese Resonansie (KMR) spektroskopiese data, sowel as gespesialiseerde funksies vir die dekonvolusie van opeenvolgende reaksie-tydreekse. Hierdie funksionaliteit was onontbeerlik vir die verdere navorsing in hierdie proefskrif. Hoofstuk 3 beskryf die ontwikkeling van ’n nuwe metodiek vir die omvangryke bepaling van ensiem-kinetiese parameters vir sisteembiologie, deur van KMR gebruik te maak. In teenstelling tot tradisionele ensiem-kinetiese essai-metodes, is hierdie nuwe metodologie minder arbeidsintensief en lewer dit beduidend meer inligting per eksperiment. Deur die kinetiese vergelykings op tydsafhanklike KMR data te pas, word dinamiese veranderinge in substrate, produkte en allosteriese effektors gekwantifiseer en hierdie inligting gebruik in die passingsprosedure. Die data bevat inligting oor ko¨operatiewe substraatbinding, omkeerbaarheid, produkinhibisie en allosteriese effekte. Die voorgestelde metodologie word toegepas op die karakterisering van die eerste twee glikolitiese ensieme. In Hoofstuk 4 word die konstruksie, parameterisering en validering van ’n aantal kinetiese modelle van glikolise in E. coli onder mikro-a¨erobiese toestande uiteengesit. Die waarde van die modelleringsbenadering lˆe in die gemak waarmee omslagtige in vivo eksperimente in silico uitgevoer kan word. Om die onderste helfte van die glikolitiese pad te modelleer word ’n soortgelyke tegniek as in Hoofstuk 3 gebruik. Modelle van die reaksies vanaf triosefosfaat-isomerase tot by pirovaat-kinase is geparameteriseer deur dit op ’n versameling 31P KMR-tydreekse te pas. Hierdie benadering brei bostaande metodologie uit tot ensiem-subnetwerke en genereer data wat die volle kompleksiteit van regulerende interaksies in die netwerk insluit. Die geverifieerde modelle word in Hoofstuk 5 noukeurig ondersoek. ’n Strukturele analise van die modeltopologie word onderneem om die elementˆere fluksie-modes van fermentatiewe glikolise in E. coli te verklaar, sowel as om ’n futiele siklus rondom fosfo¨enolpirovaat karboksilase en fosfo¨enolpirovaat karboksikinase te identifiseer. Die bestendige-toestandsgedrag en kontrole-eienskappe word in silico ondersoek onder toestande van verskeie ATP beladings en suurstofbeskikbaarheid. ’n Aantal hipoteses word voorgelˆe, wat die regulering van vry energie in E. coli, sowel as die metaboliese reaksies van E. coli onder veranderende redoks-vereistes kan verklaar. Onder andere dui die resultate daarop dat die fosfo¨enolpirovaat:fosfotransferase sisteem (wat verantwoordelik is vir glukose-opname in die sel) die glikolitiese fluksie beheer en dat E. coli onder mikro-a¨erobiese toestande die redoksbalans nie net tussen asetaat en etanol kan reguleer nie, maar ook die deur wysiging van die fluksie-balans tussen asetaat en laktaat rondom die pirovaat-formiaat-liase/laktaatdehidrogenase vertakkingspunt. Hierdie studie toon die waarde van ’n ge¨ıntegreerde rekenaarmatige en eksperimentele sisteembenadering om biologiese verskynsels te ondersoek.
Bawazir, Nada Sami. "Tuning of Plasma Membrane PI(4,5)P2 Charge Regulates Cell Migration and Glycolysis". Thesis, University of the Sciences in Philadelphia, 2020. http://pqdtopen.proquest.com/#viewpdf?dispub=27666361.
Pełny tekst źródłaVettraino, Marina Eleonora <1983>. "The inhibition of aerobic glycolysis as a therapeutic approach to improve cancer chemotherapy". Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2014. http://amsdottorato.unibo.it/6195/1/Vettraino_Marina_Eleonora_tesi.pdf.
Pełny tekst źródłaVettraino, Marina Eleonora <1983>. "The inhibition of aerobic glycolysis as a therapeutic approach to improve cancer chemotherapy". Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2014. http://amsdottorato.unibo.it/6195/.
Pełny tekst źródłaPegoraro, Caterina. "Finding novel Neural Crest regulators : Pfkfb4, a key glycolysis partner, controls Neural Crest early patterning in Xenopus laevis". Thesis, Paris 11, 2012. http://www.theses.fr/2012PA112374.
Pełny tekst źródłaNeural Crest (NC) is a transient population of multipotent cells that arises at the border between neural and non-neural ectoderm, in a region named the neural border (NB). As the neural border elevates to form the neural tube, NC cells undergo an Epithelial-To-Mesenchymal Transition (EMT), migrate extensively into the whole body to reach their final destinations and differentiate. They give rise to multiple derivatives: neurons and glia, head cartilage, bones and connective tissue, pigment cells, sympatho-adrenal cells. All these processes are regulated by the concerted actions of several genes that form a complex Gene Regulatory Network (GRN), in which many interactions have been elucidated, but even more relationships still need to be understood. Misregulation of genes normally involved in NC formation causes birth defects called neurocristopathies. Moreover, the EMT that NC cells undergo before migration also takes place when cancer cells become metastatic: the molecular events and many of the genes involved in EMT and migration are shared between NC development and cancer. The links with metastasis, neurocristopathies and the fact that still little is known about the earliest steps of NC formation, highlight the importance and the interest in understanding the Gene Regulatory Network (GRN) leading to NC formation and EMT.In the laboratory, we are interested in the early steps of NC induction and specification. In order to identify genes preferentially involved in early NC development compared to genes involved in neural and non-neural ectoderm formation, a transcriptome screen on different microdissected embryonic tissues has been performed. The validation of the results of the screen revealed several interesting genes with a potential function in NC formation. We focused particularly on two of them, due to their original function compared to the majority of the genes involved in NC development: serca1 and pfkfb4, a calcium homeostasis regulator and a glycolysis regulator respectively. We analysed the expression patterns of serca and pfkfb family genes during Xenopus laevis development. Then, due to its specific expression in NC, we studied more in details the role of pfkfb4 in NC formation. This analysis revealed that pfkfb4 is necessary for neural and neural crest specification. However, despite its known role in glycolysis, pfkfb4 morphant phenotype in Xenopus laevis embryos is not due to an alteration of the glycolytic pathway.In conclusion, our results reveal a novel extra-glycolytic role for Pfkfb4 during Xenopus laevis embryonic development
Ellingson, William J. "The effects of 3-phosphoglycerate and other metabolites on the activation of AMP-activated protein kinase by LKB1/STRAD/MO25 /". Diss., CLICK HERE for online access, 2006. http://contentdm.lib.byu.edu/ETD/image/etd1406.pdf.
Pełny tekst źródłaBaker, Jennifer Mary. "The effect of extracellular pH on human platelet metabolism". Thesis, University of Birmingham, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368422.
Pełny tekst źródłaQuinn, Gregory Bernard. "The recombinant expression and characterization of human neuron specific enolase". Thesis, University of Southampton, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.241344.
Pełny tekst źródłaCrowther, Gregory John. "An analysis of metabolic fluxes in contracting human skeletal muscle /". Thesis, Connect to this title online; UW restricted, 2002. http://hdl.handle.net/1773/10538.
Pełny tekst źródłaPoulain, Laury. "Etude du métabolisme du glucose dans les leucémies aigües myéloïdes et implication de la voie de signalisation mTORC1". Thesis, Sorbonne Paris Cité, 2016. http://www.theses.fr/2016USPCB028/document.
Pełny tekst źródłaAcute Myeloid Leukemia (AML) are heterogeneous hematological diseases with poor prognosis characterized by a clonal expansion of immature progenitors. Many deregulation of signaling pathways are found in leukemic cells and give them an advantage of proliferation and survival. The MTORC1 signaling pathway, which controls protein translation, autophagy and several metabolic pathways, is constitutively activated in leukemic cells. Metabolic reprogramming in particular the "Warburg effect" is a phenomenon well described in cancer cells. High rate of glycolysis has been considered to give tumour cells advantages through rapid production of ATP and intermediates for the synthesis of nucleotides, amino acids, and lipids. In this context, I studied glucose metabolism in AML cells and the involvement of the mTORC1 signaling pathway in the deregulation of this metabolism. First, I identified by a transcriptomic analysis in the MOLM-14 cell line that mTORC1 signaling controls several metabolic pathways including those for glucose utilization. This has been verified in several AML cell lines, since inhibition or over-activation of mTORC1 respectively induces a decrease or an increase in glucose consumption and lactate production. Interestingly, the level of activation of the mTORC1 signaling pathway determines the sensitivity of AML cells to the inhibition of glycolysis. Indeed, when mTORC1 is activated, the blockade of glycolysis induces autophagy and apoptosis of leukemic cells. Conversely, blocking mTORC1 induces metabolic reprogramming of leukemic cells, which then mainly use oxidative phosphorylation to produce ATP for their needs. AML cell survival become independent of glucose. Unlike primary AML cells, survival of normal immature hematopoietic cells CD34+ is only barely affected by the blockade of glycolysis. Thus, targeting the glucose metabolism may constitute an attractive therapeutic strategy in AML. I then investigated the anti-leukemic activity induced by the inhibition of the pentose phosphate pathway (PPP) and more particularly by the specific blockade of G6PD (glucose 6-phosphate dehydrogenase) with the 6-aminonicotinamide (6- AN) compound. Indeed, a metabolic flux analysis demonstrated that a significant proportion of glucose was directed towards the PPP. This result suggested that the addiction of leukemic cells toward glucose might be related to an increased use of PPP. I then observed that the 6-AN induced in vitro cytotoxicity including in primary AML cells from patients without effect on normal immature hematopoietic cells CD34+ and in vivo in a xenograft model of MOLM-14 cell line in the NUDE mouse. This study therefore demonstrated that the constitutive activation of mTORC1 makes AML cells survival dependent on glycolysis, and creates a specific vulnerability to the inhibition of G6PD. Given that deregulation of the mTORC1 signaling pathway is almost constant in AML, targeting G6PD may therefore represent an interesting therapeutic strategy
Ozawa, Shota. "Glycolysis, but not Mitochondria, responsible for intracellular ATP distribution in cortical area of podocytes". Kyoto University, 2017. http://hdl.handle.net/2433/217989.
Pełny tekst źródłaCampanelli, John R. (John Richard). "The kinetics of hydrolysis and glycolysis of poly(ethylene terephthalate) melts at high temperatures". Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41312.
Pełny tekst źródłaAn end-group analysis method was developed involving the titration of carboxyl groups of hydrolysate samples dissolved in one of two solvent systems, dimethylphenol:chloroform or dimethylsulphoxide, depending on reaction extent.
A kinetic model was developed and found to provide a good fit with experimental data and with equilibrium concentrations. The activation energy of hydrolysis was calculated to be 56 kJ/mol. Zn$ sp{+2}$ catalysts increased the hydrolysis rate constant by about 20% at the same reaction temperature.
PET glycolysis was studied at the same conditions of temperature and initial reactor loading as for hydrolysis using a method based on the removal of unreacted ethylene glycol from the product mixture through careful drying. The activation energy of glycolysis was found to be 102 kJ/mol. Glycolysis rate constants were about 7 times greater than the corresponding hydrolysis rate constants. The difference between the sets of rate constants is attributed largely to the morphology of the respective reaction mixtures (solution/emulsion).
Doyen, Jérôme. "Rôle des protéines de régulation du pH intracellulaire et du métabolisme énergétique dans les carcinomes du sein triple négatif". Electronic Thesis or Diss., Nice, 2013. http://www.theses.fr/2013NICE4147.
Pełny tekst źródłaAgressive cancers often harbor an exacerbated glycolytic metabolism with overexpression of proteins that maintain intracellular pH by extruding metabolic acid waste (via CAIX, CAXII, MCT1 and MCT4 among others). The "triple-negative" breast cancers (with no expression of estrogen, progesteron and Her-2 receptors) have an increased consumption of glucose and worse prognosis in comparison with other breast cancers. Immunohistochemical analysis of glycolytic proteins among 159 patients with TNEG breast cancer, showed that MCT4 was predictive for metastasis and death occurence. In vitro targeting of MCT4 by Zinc Finger Nuclease (ZFN) technique demonstrated an anti-proliferative effect. However, the maximal anti-proliferative effect was observed with the combination of MCT4/MCT1 (by pharmacological inhibition) and mitochondrial respiration by phenformine in the Hs578t TNEG cell line. This study demonstrated that targeting glycolytic protein could have a therapeutic effect in TNEG breast cancers. Another study also use targeting of glycolytic protein such as CAIX and CAXII to increase in vitro and in vivo radiosensitivity of colorectal cell lines while demonstrating an original mechanism of radiosensitization by increasing intracellular acidosis. Finally we demonstrated that the hypoxamiR miR210 was involved in the radioresistance of lung cancer cell line with a stronger impact than the oxygen effect
Shao, Wei 1970. "Identification of caspase-1 and caspase-3 substrates and study on caspase-1 substrates in glycolytic pathway". Thesis, McGill University, 2007. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=100248.
Pełny tekst źródłaWilliams, Andrew C. "Glucose metabolism in human spermatozoa". Thesis, University of Bristol, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302101.
Pełny tekst źródłaPatterson, Bly Addison. "Pectoralis muscle of turkey displays divergent function as correlated with meat quality". Thesis, Virginia Tech, 2015. http://hdl.handle.net/10919/52929.
Pełny tekst źródłaMaster of Science