Gotowe bibliografie tematyczne / Genetic technology

Gotowa bibliografia na temat „Genetic technology”

Autor: Grafiati
Data publikacji: 4 czerwca 2021
Data aktualizacji: 20 lutego 2023

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Artykuły w czasopismach na temat "Genetic technology"

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EDWARDS, A., i C. CASKEY. "Genetic marker technology". Current Opinion in Biotechnology 2, nr 6 (grudzień 1991): 818–22. http://dx.doi.org/10.1016/s0958-1669(05)80113-2.

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Lesser, William H., i Anatole F. Krattiger. "What is 'Genetic Technology'?" Biodiversity Letters 2, nr 2 (marzec 1994): 31. http://dx.doi.org/10.2307/2999665.

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Jinjin, Chen, i Li Raojuan. "Patent in genetic technology". International Journal of Liability and Scientific Enquiry 1, nr 4 (2008): 402. http://dx.doi.org/10.1504/ijlse.2008.018287.

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Grossman, Margaret Rosso. "Genetic Technology and Food Security". American Journal of Comparative Law 62, nr 1 (1.07.2014): 273–302. http://dx.doi.org/10.5131/ajcl.2013.0025.

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Tabor, John M. "Principles of Genetic Engineering Technology". Drug Development and Industrial Pharmacy 11, nr 5 (styczeń 1985): 1073–88. http://dx.doi.org/10.3109/03639048509055598.

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Gibson, R. K. "The technology of genetic manipulation". Journal of Applied Bacteriology 63 (grudzień 1987): 7s—19s. http://dx.doi.org/10.1111/j.1365-2672.1987.tb03607.x.

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LAYMAN, PATRICIA. "Swiss voters endorse genetic technology". Chemical & Engineering News 76, nr 24 (15.06.1998): 8–9. http://dx.doi.org/10.1021/cen-v076n024.p008a.

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Skodbo, Sara. "Enrolling genetic technology in regulation". Focaal 2005, nr 46 (1.12.2005): 91–106. http://dx.doi.org/10.3167/092012906780786825.

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This article addresses the need to overcome theoretical weaknesses of both technologically and socially deterministic accounts of technological development. Technology does not simply 'impact' on local contexts, but nor does it act as a tabula rasa, subject to the free attribution of meaning by local social actors. Expanding on theoretical developments in the anthropology of art (Gell 1998) and gender and technology (Strathern 1988, 1999, 2001), the essay seeks to explore genetic technology as a social agent and as a technological 'index'. Examining a case of genetic technology regulation and innovation in Norway, the article argues that technology is best understood as an agent that is engaged with on an affective basis by those who interact with it.
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Nicholas, F. W. "Genetic improvement through reproductive technology". Animal Reproduction Science 42, nr 1-4 (kwiecień 1996): 205–14. http://dx.doi.org/10.1016/0378-4320(96)01511-4.

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Gentry, Deborah B. "Genetic technology and family conflict". Mediation Quarterly 18, nr 1 (wrzesień 2000): 5–17. http://dx.doi.org/10.1002/crq.3890180103.

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Rozprawy doktorskie na temat "Genetic technology"

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Yu, Bin, i 于斌. "Study of recombineering technology in Salmonella and its applications". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hdl.handle.net/10722/211555.

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In the past few years, in vivo recombination technologies have emerged to improve the efficiency and simplicity of genetic engineering in Escherichia coli, Salmonella enterica serovar, and other gram-negative bacteria. Phage λ Red homologous recombination system is used to mediate the accurate replacement of target DNA with PCR-generated ?targeting cassettes? that contain flanking regions of shared homologous DNA sequence. However, the efficiency of λ Red-mediated recombineering in Salmonella is far lower than that in Escherichia coli. In this study, I firstly improved the recombineering-based strategy by using linear DNA targeting cassettes that contain long flanking ?arms? of sequence (ca. 1,000 base pairs) homologous to the chromosomal target. This reliable and efficient method enables multiple gene targeting procedures to be performed on a single Salmonella enterica serovar typhi Ty21a (Ty21a) chromosome in a straightforward, sequential manner with high efficiency. Secondly, I applied this improved strategy in construction of Salmonella to be live attenuated oral vaccine and tumor targeting vector. In the first part of this thesis, I describe an improved method in Ty21a. Using this strategy, I inserted three different influenza antigen expression cassettes as well as a green fluorescent protein reporter gene into four different loci on the Ty21a chromosome with high efficiency and accuracy. Fluorescent microscopy and Western blotting analysis confirmed that strong inducible expression of all four heterologous genes could be achieved. The immune response of this vaccine was also evaluated by ELISA and ELIspots. In the second part of this thesisi, I use this improved recombineering strategy to engineer bacteria of Salmonella typhimurium (S. typhimurium) as therapeutic agents against solid tumor. In current study, a major challenge for bacterial therapy of cancer is avoiding damage to normal tissues. Consequently the virulence of bacteria must be adequately attenuated for therapeutic use. An alternative approach was developed here. By placing an essential gene under a hypoxia conditioned promoter, S. typhimurium strain SL7207 was engineered to generate strain YB1 that survives only in anaerobic conditions without otherwise affecting its functions. In breast and liver tumor bearing mice models, YB1 grew within tumor, retarding its growth, while being rapidly eliminated from normal tissues. Mice treated with SL7207 were killed by infection within short period time. Inhibition of tumor growth by YB1 was significant and was enhanced by the addition of 5-FU in breast cancer model. The development of an “obligate” anaerobic Salmonella provides a much safer bacterial vector for further development of anti-tumor therapies without compromising the other functions or tumor fitness of the bacterium as attenuation methods normally do. In summary, I have developed an efficient, robust and versatile method in genome-wide Salmonella genetic manipulation. Furthermore, I used this method to construct a recombinant Ty21a antigen-expressing vaccine strain and a tumor targeting YB1 strain.
published_or_final_version
Biochemistry
Doctoral
Doctor of Philosophy
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Hultin, Emilie. "Genetic Sequence Analysis by Microarray Technology". Doctoral thesis, Stockholm : School of Biotechnology, Royal Institute of Technology, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4330.

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Lindroos, Katarina. "Accessing Genetic Variation by Microarray Technology". Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2002. http://publications.uu.se/theses/91-554-5251-5/.

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Freethy, Randy J. "The ethics of genomic technology". Theological Research Exchange Network (TREN), 2005. http://www.tren.com/search.cfm?p001-1054.

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Weston, Delys E. "Democracy and political economy of genetic engineering /". Access via Murdoch University Digital Theses Project, 2007. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20070327.143205.

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Neadeau, Joseph Francis. "Comparing Genetic Modification and Genetic Editing Technolgies: Minimal Required Acreage". Thesis, North Dakota State University, 2018. https://hdl.handle.net/10365/29878.

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There are many technologies being developed for crop breeding. Two interesting technologies are genetic modification and genetic editing. Competitive pressures and changing consumer preferences are forcing organizations to invest heavily in these two technologies. Organizations must decide which traits they want to target and must commit significant time a money to the project. Traditionally, firms would decide which project to embark on if the project is net present value positive. Throughout the research and development process managers have flexibility to abandon the project once new information is received. That flexibility has value and real option analysis must be performed to value that flexibility. Once the value of a GM and GE project is determined, how might an organization decide which project to do? The concept of minimum required acreage (MRA) is developed in this study, allowing organizations to compare GM and GE technologies and decide which project to invest it.
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Zhuang, Nan. "Logic synthesis and technology mapping using genetic algorithms". Thesis, Imperial College London, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.286760.

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Asadi, Romisa. "Development of genetic control technology for Tephritid pests". Thesis, Cardiff University, 2015. http://orca.cf.ac.uk/72611/.

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The olive fly, Bactrocera oleae, is the single most important pest in olive plantations. Currently, control of olive fly relies on the heavy use of chemical pesticides. The sterile insect technique (SIT) is a highly effective, species-specific and environmentally non-polluting method of pest control that involves the mass-release of sterilised insects. SIT is considered a potentially valuable method for the control of olive fly. Previous olive fly SIT attempts failed due to an inability to produce large numbers of flies, low egg production rates and lack of a method to separate the sexes. RIDL (Release of Insects carrying a Dominant Lethal) is a biotechnology-based variant of SIT. This could potentially overcome several problems of classical SIT, including the radiation damage to insects. To develop fly male sterility, we have identified and tested several different germline specific promoters and several potential effector genes. These have been linked to the ‘tet-off’ expression system, which is suppressed by dietary tetracycline, and were initially tested in the Mediterranean fruit fly (Ceratitis capitata) for practicality. In the absence of tetracycline, tTAV binds to its target sequence, tetO, and activates expression of downstream genes. Flies carrying a promoter construct (topi-tTAV or β2-tubulin-tTAV) in medfly were crossed to flies carrying effector constructs (tetO-I-ppoI, tetO-3zincfinger or tetO-ProtamineFokI). A combination of β2-tubulin-tTAV and tetO-ProtamineFokI gave the best male sterility in medfly. A construct containing both elements was designed, and transposon-based germline transformation was used to generate and test ten olive fly strains. Progeny assessment off tetracycline indicates high penetrance of the male-sterile phenotype in all strains, with only 0.0-2.4% viable progeny; this sterile phenotype appears to be completely suppressed by provision of dietary tetracycline.
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Veikondis, Rene. "Genetic characterisation of fungal disease resistance genes in grapevine using molecular marker technology". Thesis, Stellenbosch : Stellenbosch University, 2014. http://hdl.handle.net/10019.1/96090.

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Thesis (MSc)--Stellenbosch University, 2014.
ENGLISH ABSTRACT: The aim of this study on grapevine was to genetically characterise, validate and map the reported fungal disease resistance genes of Pölöskei Muskotály (PM), Kishmish Vatkana (KV) and Villard Blanc (VB) in South Africa using QTL analysis. These fungal resistant parents were crossed with other varieties that have desirable fruit qualities in an effort to combine fungal disease resistance with desirable fruit qualities in a single variety. The genetic basis of PM’s resistance to downy and powdery mildew has not been investigated before. It does however have VB in its pedigree so the assumption was made that the same QTL/genes present in VB contribute to this resistance. KV’s resistance to powdery mildew reportedly originates from the REN1 gene located on chromosome 13. VB’s powdery and downy mildew resistance is conferred by QTL present on chromosome 15 and chromosome 18 respectively and has been reported in numerous studies. The study populations comprised of 124 F1 PM x Regal Seedless plants, 16 F1 PM x G4-3418 plants, 14 F1 PM x Sunred Seedless plants, 158 F1 Sunred Seedless x KV plants and 250 F1 VB x G1-6604 plants. DNA was extracted from the leaves and all plants were screened using microsatellite markers. Phenotypic evaluations of downy and/or powdery mildew resistance were performed on the appropriate populations. The molecular data was used to generate linkage maps and combined with phenotypic data to perform QTL analysis. From the molecular data generated for the three PM populations it was determined that the F1 progeny inherited almost exclusively maternal alleles, and could not be used in a mapping study. These populations were eliminated from the study and PM will be used as a pollen donor in future. Molecular data from the Sunred Seedless x KV cross was used to generate a linkage map for chromosome 13 comprising eight markers and spanning 45.6 cM. When combined with the data from two powdery mildew phenotypic screens a QTL peak spanning the REN1 gene on chromosome 13 of KV was identified. This locus explains between 44.8% and 57.7% of the phenotypic variance observed. The molecular data from the VB x G1-6604 cross was used to generate partial linkage maps for chromosome 15 and 18. Eleven markers were mapped on chromosome 15 spanning 56.4 cM, and ten markers were mapped on chromosome 18 spanning 101.8 cM. When the chromosome 15 linkage map was combined with the data from two powdery mildew phenotypic screens a QTL associated with powdery mildew resistance was identified on chromosome 15 that explains between 18.9% and 23.9% of the phenotypic variance observed. Likewise a QTL associated with downy mildew resistance was identified on chromosome 18 when the chromosome 18 linkage map was combined with data from two downy mildew phenotypic screens. This QTL explains between 19.1% and 21.2% of the phenotypic variance observed. This study succeeded in genetically characterising the fungal disease resistance genes of two different sources of grapevine and provided exclusionary information on a third resistance source for future breeding applications.
AFRIKAANSE OPSOMMING: Die doel van hierdie studie in wingerd was om die genetiese komponent van die swamweerstandsgene van Pölöskei Muskotály (PM), Kishmish Vatkana (KV) and Villard Blanc (VB) in Suid-Afrika te karakteriseer en die teenwoordigheid daarvan te bevestig deur ʼn Kwantitatiewe Eienskap Lokus (KEL) benadering te volg. In ʼn poging om swamweerstand en goeie vrugeienskappe te kombineer in ʼn enkel variëteit is die weerstandige variëteite met vatbare variëteite gekruis wat goeie vrugeienskappe besit. Die genetiese basis van PM se weerstand teen donsskimmel en witroes is nog nie vantevore bestudeer nie. VB is een van sy voorgeslagte en daar is aangeneem dat dieselfde KEL/gene waarskynlik verantwoordelik is vir die weerstand. Dit is gerapporteer dat KV se witroesweerstand afkomstig is van die REN1 geen op chromosoom 13. Vele publikasies rapporteer VB se weerstand teen witroes en donsskimmel Beide die witroes- en donsskimmelweerstand word oorgedra deur KEL teenwoordig op chromosome 15 en 18 onderskeidelik. Die populasies gebruik in hierdie studie het bestaan uit 124 F1 PM x Regal Seedless plante, 16 F1 PM x G4-3418 plante, 14 F1 PM x Sunred Seedless, 158 F1 Sunred Seedless x KV plante en 250 F1 VB x G1-6604 plante onderskeidelik. Blare is versamel vir DNS isolasie en genotipering met mikrosatellietmerkers. Al drie populasies se weerstand teen donsskimmel en/of witroes is fenotipies geëvalueer. Die molekulêre data is gebruik om genetiese koppelingskaarte op te stel en gekombineer met die fenotipiese data om KEL analise uit te voer. Die molekulêre data van die drie PM populasies het daarop gedui dat die F1 nageslag amper uitsluitlik moederlike allele geërf het en kon gevolglik nie gebruik word in die studie nie. Die PM populasies is uitgesluit uit hierdie studie en PM sal voortaan as stuifmeelskenker gebruik word. Molekulêre data van die Sunred Seedless x KV kruising is gebruik om ʼn koppelingskaart vir chromosoom 13 op te stel wat 45.6 cM lank is en agt merkers bevat. Die KEL analise van die koppelingskaart en twee fenotipiese datastelle vir witroes het ʼn KEL piek geïdentifiseer wat oor die lengte van die REN1 geen-interval strek. Hierdie lokus is verantwoordelik vir 44.8% tot 57.7% van die fenotipiese variasie wat waargeneem word. Molekulêre data van die VB x G1-6604 kruising is gebruik om gedeeltelike koppelingskaarte vir chromosome 15 en 18 op te stel. Elf merkers karteer op die chromosoom 15 kaart van 56.4 cM en tien merkers karteer op die chromosoom 18 kaart van 101.8 cM. KEL analise van chromosoom 15 se koppelingskaart en twee witroes fenotipiese datastelle het ʼn KEL geïdentifiseer wat 18.9% tot 23.9% van die fenotipiese variasie verduidelik. ʼn KEL is ook op chromosoom 18 geïdentifiseer wat 19.1% tot 21.2% van die fenotipiese variasie verduidelik met die gekombineerde analise van chromosoom 18 se koppelingskaart en twee donsskimmel fenotipiese datastelle. Hierdie studie het die genetiese komponent van die swamweerstandsgene van twee Vitis variëteite suksesvol gekarakteriseer en bevestig. Waardevolle telingsinligting oor die derde variëteit is ook onthul.
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Johansson, Magnus. "Financial application of genetic programming". Thesis, Linköping University, Department of Computer and Information Science, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-17091.

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Książki na temat "Genetic technology"

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Using genetic technology. Oxford: Heinemann Library, 2009.

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Solway, Andrew. Using genetic technology. Chicago: Heinemann Library, 2008.

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Smith, Terry L. Modern genetic science: New technology, new decisions. New York: Rosen, 2009.

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Newton, David E. DNA technology: A reference handbook. Santa Barbara, Calif: ABC-CLIO, 2010.

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Newton, David E. DNA technology: A reference handbook. Santa Barbara, Calif: ABC-CLIO, 2009.

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Newton, David E. DNA technology: A reference handbook. Santa Barbara, Calif: ABC-CLIO, 2009.

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Newton, David E. DNA technology: A reference handbook. Santa Barbara, Calif: ABC-CLIO, 2009.

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DNA technology: A reference handbook. Santa Barbara, Calif: ABC-CLIO, 2010.

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Norer, Roland, red. Genetic Technology and Food Safety. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-23995-8.

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Miami Bio/Technology Winter Symposium (1994). Advances in gene technology: Molecular biology and human disease : proceedings of the 1994 Miami Bio/Technology Winter Symposium. Oxford: IRL Press at Oxford University Press, 1994.

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Części książek na temat "Genetic technology"

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Fost, Norman. "Regulating Genetic Technology". W Genetics and the Law III, 15–21. Boston, MA: Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-4952-5_2.

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Wu, Josephine, Tao Feng, Ruliang Xu, Fei Ye, Bruce E. Petersen, Liang Cheng i David Y. Zhang. "Diagnostic Methodology and Technology". W Molecular Genetic Pathology, 65–131. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-405-6_3.

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Baig, Hasan, i Jan Madsen. "Technology Mapping of Genetic Circuits". W Genetic Design Automation, 81–101. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-52355-8_6.

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Wilfried, Dalemans. "Gene Therapy and Genetic Vaccination: Two Hallmarks of Genetic Medicine". W Animal Cell Technology, 33–35. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5404-8_5.

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Hiatt, William R., Matthew Kramer i Raymond E. Sheehy. "The Application of Antisense RNA Technology to Plants". W Genetic Engineering, 49–63. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4615-7084-4_4.

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Baran, George R., Mohammad F. Kiani i Solomon Praveen Samuel. "Genetic Engineering". W Healthcare and Biomedical Technology in the 21st Century, 383–416. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4614-8541-4_12.

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Neumann, Karl-Hermann, Ashwani Kumar i Jafargholi Imani. "Genetic Problems and Gene Technology". W Plant Cell and Tissue Culture – A Tool in Biotechnology, 337–435. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-49098-0_13.

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Kennedy, B. W., i L. R. Schaeffer. "Reproductive Technology and Genetic Evaluation". W Advances in Statistical Methods for Genetic Improvement of Livestock, 507–32. Berlin, Heidelberg: Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-74487-7_23.

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Neumann, Karl-Hermann, Jafargholi Imani i Ashwani Kumar. "Genetic Problems and Gene Technology". W Plant Cell and Tissue Culture - A Tool in Biotechnology, 249–85. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-93883-5_13.

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Lucca, Paolo, i Ingo Potrykus. "Genetic engineering technology against malnutrition". W Plant Tissue Culture, 167–74. Vienna: Springer Vienna, 2003. http://dx.doi.org/10.1007/978-3-7091-6040-4_10.

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Streszczenia konferencji na temat "Genetic technology"

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Schwarz, Tobias, i Christian Hochberger. "Technology Mapping of Genetic Circuits". W ICCAD '22: IEEE/ACM International Conference on Computer-Aided Design. New York, NY, USA: ACM, 2022. http://dx.doi.org/10.1145/3508352.3549344.

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Pandey, Hari Mohan, Anurag Dixit i Deepti Mehrotra. "Genetic algorithms". W the CUBE International Information Technology Conference. New York, New York, USA: ACM Press, 2012. http://dx.doi.org/10.1145/2381716.2381766.

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Neubauer, A. "Genetic algorithms in automatic fire detection technology". W Second International Conference on Genetic Algorithms in Engineering Systems. IEE, 1997. http://dx.doi.org/10.1049/cp:19971177.

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Suzuki, Masaki, Taro Matsumaru, Setsuo Tsuruta, Rainer Knauf, Takaaki Motomura i Yoshitaka Sakurai. "A case based approach for an intelligent route optimization technology". W GECCO '14: Genetic and Evolutionary Computation Conference. New York, NY, USA: ACM, 2014. http://dx.doi.org/10.1145/2598394.2605676.

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Galvan, Edgar, i Richard Malak. "A Genetic Algorithm Approach for Technology Characterization". W ASME 2012 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/detc2012-70465.

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It is important for engineers to understand the capabilities and limitations of the technologies they consider for use in their systems. Several researchers have investigated approaches for modeling the capabilities of a technology with the aim of supporting the design process. In these works, the information about the physical form is typically abstracted away. However, the efficient generation of an accurate model of technical capabilities remains a challenge. Pareto frontier based methods are often used but yield results that are of limited use for subsequent decision making and analysis. Models based on parameterized Pareto frontiers—termed Technology Characterization Models (TCMs)—are much more reusable and composable. However, there exists no efficient technique for modeling the parameterized Pareto frontier. The contribution of this paper is a new algorithm for modeling the parameterized Pareto frontier to be used as a model of the characteristics of a technology. The proposed algorithm uses fundamental concepts from multiobjective genetic optimization and machine learning to generate a model of the technology frontier.
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Ben Ameur, Mohamed sadek, Anis Sakly i Abdellatif Mtibaa. "Implementation of genetic algorithms using FPGA technology". W the Annual FPGA Conference. New York, New York, USA: ACM Press, 2012. http://dx.doi.org/10.1145/2451636.2451639.

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Tan, Chong, Ying Sun, Gongfa Li, Bo Tao, Shuang Xu i Fei Zeng. "Image Segmentation Technology Based on Genetic Algorithm". W the 2019 3rd International Conference. New York, New York, USA: ACM Press, 2019. http://dx.doi.org/10.1145/3316551.3318229.

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Mu, Dongzhou, Chao Xu i Hongmei Ge. "Hybrid Genetic Algorithm Based Image Enhancement Technology". W 2011 International Conference on Internet Technology and Applications (iTAP). IEEE, 2011. http://dx.doi.org/10.1109/itap.2011.6006336.

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Patel, Devesh. "Filter selection using genetic algorithms". W Electronic Imaging: Science & Technology, redaktorzy Nasser M. Nasrabadi i Aggelos K. Katsaggelos. SPIE, 1996. http://dx.doi.org/10.1117/12.234245.

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Chen, Yen-Wei, Zensho Nakao i Shinichi Tamura. "Blind deconvolution by genetic algorithms". W Electronic Imaging: Science & Technology, redaktorzy Edward R. Dougherty, Jaakko T. Astola i Harold G. Longbotham. SPIE, 1996. http://dx.doi.org/10.1117/12.235831.

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Raporty organizacyjne na temat "Genetic technology"

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Ann Holmes, Ann Holmes. Adapting genetic technology for marine conservation. Experiment, maj 2022. http://dx.doi.org/10.18258/26843.

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Kausch, Albert, i Richard Rhodes. Research and Technology Development for Genetic Improvement of Switchgrass. Office of Scientific and Technical Information (OSTI), maj 2017. http://dx.doi.org/10.2172/1357908.

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Ozcelik, Hilmi, i Julia A. Knight. Microarray Technology to Study the Role of Genetic Polymorphisms in Breast Cancer Risk. Fort Belvoir, VA: Defense Technical Information Center, lipiec 2002. http://dx.doi.org/10.21236/ada406970.

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Geller, Melissa A., Hee Y. Lee, Kristin Niendorf, Rachel I. Vogel i Heewon Lee. Mobile Phone Technology to Increase Genetic Counseling for Women with Ovarian Cancer and Their Families. Fort Belvoir, VA: Defense Technical Information Center, czerwiec 2015. http://dx.doi.org/10.21236/ada621258.

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Trottier, R. W., F. C. Hodgin, M. Imara, D. Phoenix, S. Lybrook, L. A. Crandall, R. E. Moseley i D. Armotrading. Impact of human genome initiative-derived technology on genetic testing, screening and counseling: Cultural, ethical and legal issues. Progress report. Office of Scientific and Technical Information (OSTI), marzec 1993. http://dx.doi.org/10.2172/10134803.

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Gera, Abed, Abed Watad, P. Ueng, Hei-Ti Hsu, Kathryn Kamo, Peter Ueng i A. Lipsky. Genetic Transformation of Flowering Bulb Crops for Virus Resistance. United States Department of Agriculture, styczeń 2001. http://dx.doi.org/10.32747/2001.7575293.bard.

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Objectives. The major aim of the proposed research was to establish an efficient and reproducible genetic transformation system for Easter lily and gladiolus using either biolistics or Agrobacterium. Transgenic plants containing pathogen-derived genes for virus resistance were to be developed and then tested for virus resistance. The proposal was originally aimed at studying cucumber mosaic virus (CMV) resistance in plants, but studies later included bean yellow mosaic virus (BYMV). Monoclonal antibodies were to be tested to determine their effectiveness in interning with virus infection and vector (aphid) transmission. Those antibodies that effectively interfered with virus infection and transmission were to be cloned as single chain fragments and used for developing transgenic plants with the potential to resist virus infection. Background to the topic. Many flower crops, as lily and gladiolus are propagated vegetatively through bulbs and corms, resulting in virus transmission to the next planting generation. Molecular genetics offers the opportunity of conferring transgene-mediated disease resistance to flower crops that cannot be achieved through classical breeding. CMV infects numerous plant species worldwide including both lilies and gladioli. Major conclusions, solutions and achievements. Results from these for future development of collaborative studies have demonstrated the potential transgenic floral bulb crops for virus resistance. In Israel, an efficient and reproducible genetic transformation system for Easter lily using biolistics was developed. Transient as well as solid expression of GUS reporter gene was demonstrated. Putative transgenic lily plantlets containing the disabled CMV replicase transgene have been developed. The in vitro ability of monoclonal antibodies (mAbs) against CMV to neutralize virus infectivity and block virus transmission by M. persicae were demonstrated. In the US, transgenic Gladiolus plants containing either the BYMV coat protein or antisense coat protein genes have been developed and some lines were found to be virus resistant. Long-term expression of the GUS reporter gene demonstrated that transgene silencing did not occur after three seasons of dormancy in the 28 transgenic Gladiolus plants tested. Selected monoclonal antibody lines have been isolated, cloned as single chain fragments and are being used in developing transgenic plants with CMV resistance. Ornamental crops are multi-million dollar industries in both Israel and the US. The increasing economic value of these floral crops and the increasing ban numerous pesticides makes it more important than ever that alternatives to chemical control of pathogens be studied to determine their possible role in the future. The cooperation resulted in the objectives being promoted at national and international meetings. The cooperation also enabled the technology transfer between the two labs, as well as access to instrumentation and specialization particular to the two labs.
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Комарова, Олена Володимирівна, i Альберт Армаїсович Азарян. Computer Simulation of Biological Processes at the High School. CEUR Workshop Proceedings (CEUR-WS.org), 2018. http://dx.doi.org/10.31812/123456789/2695.

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Abstract. Research goals: the necessity of study in high school of the law of Hardy – Weinberg as one of the fundamental genetic laws was justified. The peculiarities of using the method of model experiment in the study of the genetic and evolutionary processes in populations with the use of computer technology. Object of research: computer simulation of population genetic structure. Subject of research: computer simulation of genetic and evolutionary processes in ideal and real populations. Research methods: pedagogical experiment (survey), analysis of scientific publications on the use of the high school method of modelling genetic and evolutionary processes in populations, computer simulation. Results of the research: a web page for processing by the pupils of the modelling results of genetic and evolutionary processes in populations was created.
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Комарова, Олена Володимирівна, i Альберт Арамаїсович Азарян. Computer Simulation of Biological Processes at the High School. CEUR-WS.org, 2018. http://dx.doi.org/10.31812/123456789/2656.

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Research goals: the necessity of study in high school of the law of Hardy – Weinberg as one of the fundamental genetic laws was justified. The peculiarities of using the method of model experiment in the study of the genetic and evolutionary processes in populations with the use of computer technology. Object of research: computer simulation of population genetic structure. Subject of research: computer simulation of genetic and evolutionary processes in ideal and real populations. Research methods: pedagogical experiment (survey), analysis of scientific publications on the use of the high school method of modelling genetic and evolutionary processes in populations, computer simulation. Results of the research: a web page for processing by the pupils of the modelling results of genetic and evolutionary processes in populations was created.
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Dawson, William O., i Moshe Bar-Joseph. Creating an Ally from an Adversary: Genetic Manipulation of Citrus Tristeza. United States Department of Agriculture, styczeń 2004. http://dx.doi.org/10.32747/2004.7586540.bard.

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Citrus is one of the major agricultural crops common to Israel and the United States, important in terms of nutrition, foreign exchange, and employment. The economy of both citrus industries have been chronically plagued by diseases caused by Citrus tristeza virus (CTV). The short term solution until virus-resistant plants can be used is the use of mild strain cross-protection. We are custom designing "ideal" protecting viruses to immunize trees against severe isolates of CTV by purposely inoculating existing endangered trees and new plantings to be propagated as infected (protected) citrus budwood. We crossed the substantial technological hurdles necessary to accomplish this task which included developing an infectious cDNA clone which allows in vitro manipulation of the virus and methods to then infect citrus plants. We created a series of hybrids between decline-inducing and mild CTV strains, tested them in protoplasts, and are amplifying them to inoculate citrus trees for evaluation and mapping of disease determinants. We also extended this developed technology to begin engineering transient expression vectors based on CTV as tools for genetic improvement of tree crops, in this case citrus. Because of the long periods between genetic transformation and the ultimate assay of mature tree characteristics, there is a great need for an effective system that allows the expression or suppression of target genes in fruiting plants. Virus-based vectors will greatly expedite progress in citrus genetic improvement. We characterized several components of the virus that provides necessary information for designing virus-based vectors. We characterized the requirements of the 3 ’-nontranslated replication promoter and two 3 ’-ORF subgenomic (sg) mRNA controller elements. We discovered a novel type of 5’-terminal sgRNAs and characterized the cis-acting control element that also functions as a strong promoter of a 3 ’-sgRNA. We showed that the p23 gene controls negative-stranded RNA synthesis and expression of 3 ’ genes. We identified which genes are required for infection of plants, which are host range determinants, and which are not needed for plant infection. We continued the characterization of native dRNA populations and showed the presence of five different classes including class III dRNAs that consists of infectious and self-replicating molecules and class V dRNAs that contain all of the 3 ’ ORFs, along with class IV dRNAs that retain non-contiguous internal sequences. We have constructed and tested in protoplasts a series of expression vectors that will be described in this proposal.
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Gur, Amit, Edward Buckler, Joseph Burger, Yaakov Tadmor i Iftach Klapp. Characterization of genetic variation and yield heterosis in Cucumis melo. United States Department of Agriculture, styczeń 2016. http://dx.doi.org/10.32747/2016.7600047.bard.

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Project objectives: 1) Characterization of variation for yield heterosis in melon using Half-Diallele (HDA) design. 2) Development and implementation of image-based yield phenotyping in melon. 3) Characterization of genetic, epigenetic and transcriptional variation across 25 founder lines and selected hybrids. The epigentic part of this objective was modified during the course of the project: instead of characterization of chromatin structure in a single melon line through genome-wide mapping of nucleosomes using MNase-seq approach, we took advantage of rapid advancements in single-molecule sequencing and shifted the focus to Nanoporelong-read sequencing of all 25 founder lines. This analysis provides invaluable information on genome-wide structural variation across our diversity 4) Integrated analyses and development of prediction models Agricultural heterosis relates to hybrids that outperform their inbred parents for yield. First generation (F1) hybrids are produced in many crop species and it is estimated that heterosis increases yield by 15-30% globally. Melon (Cucumismelo) is an economically important species of The Cucurbitaceae family and is among the most important fleshy fruits for fresh consumption Worldwide. The major goal of this project was to explore the patterns and magnitude of yield heterosis in melon and link it to whole genome sequence variation. A core subset of 25 diverse lines was selected from the Newe-Yaar melon diversity panel for whole-genome re-sequencing (WGS) and test-crosses, to produce structured half-diallele design of 300 F1 hybrids (MelHDA25). Yield variation was measured in replicated yield trials at the whole-plant and at the rootstock levels (through a common-scion grafted experiments), across the F1s and parental lines. As part of this project we also developed an algorithmic pipeline for detection and yield estimation of melons from aerial-images, towards future implementation of such high throughput, cost-effective method for remote yield evaluation in open-field melons. We found extensive, highly heritable root-derived yield variation across the diallele population that was characterized by prominent best-parent heterosis (BPH), where hybrids rootstocks outperformed their parents by 38% and 56 % under optimal irrigation and drought- stress, respectively. Through integration of the genotypic data (~4,000,000 SNPs) and yield analyses we show that root-derived hybrids yield is independent of parental genetic distance. However, we mapped novel root-derived yield QTLs through genome-wide association (GWA) analysis and a multi-QTLs model explained more than 45% of the hybrids yield variation, providing a potential route for marker-assisted hybrid rootstock breeding. Four selected hybrid rootstocks are further studied under multiple scion varieties and their validated positive effect on yield performance is now leading to ongoing evaluation of their commercial potential. On the genomic level, this project resulted in 3 layers of data: 1) whole-genome short-read Illumina sequencing (30X) of the 25 founder lines provided us with 25 genome alignments and high-density melon HapMap that is already shown to be an effective resource for QTL annotation and candidate gene analysis in melon. 2) fast advancements in long-read single-molecule sequencing allowed us to shift focus towards this technology and generate ~50X Nanoporesequencing of the 25 founders which in combination with the short-read data now enable de novo assembly of the 25 genomes that will soon lead to construction of the first melon pan-genome. 3) Transcriptomic (3' RNA-Seq) analysis of several selected hybrids and their parents provide preliminary information on differentially expressed genes that can be further used to explain the root-derived yield variation. Taken together, this project expanded our view on yield heterosis in melon with novel specific insights on root-derived yield heterosis. To our knowledge, thus far this is the largest systematic genetic analysis of rootstock effects on yield heterosis in cucurbits or any other crop plant, and our results are now translated into potential breeding applications. The genomic resources that were developed as part of this project are putting melon in the forefront of genomic research and will continue to be useful tool for the cucurbits community in years to come.
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