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Thomas, Lee. "Genetic methods for Rapid Detection of Medically Important Nosocomial Bactera". Thesis, The University of Sydney, 2007. http://hdl.handle.net/2123/3575.
Pełny tekst źródłaThomas, Lee. "Genetic methods for Rapid Detection of Medically Important Nosocomial Bactera". University of Sydney, 2007. http://hdl.handle.net/2123/3575.
Pełny tekst źródłaThe role of the microbiology laboratory is (1) to provide infection control information, so that highly transmissible isolates may be identified and appropriate control measures instigated as rapidly as possible and (2) to provide adequate information to the clinician enabling correct antibiotic choices to be made, particularly in the critically ill. Microbiological data is by definition slow as it is culture dependent: this study focused on the development of genetic, culture-independent methods for detection of resistance in nosocomial pathogens that could be introduced into the routine microbiology department and would fit into the routine workflow with a consequent reduction in time to result. Initially a duplex real-time polymerase chain reaction was developed for the rapid identification and detection of S. aureus and methicillin-resistance. This was optimised for immediate as-needs testing of positive blood cultures signalling with “Gram positive cocci, possibly staphylococcus” evident on Gram stain, on a random access real-time PCR platform. This technology, allowing early identification of S. aureus and its susceptibility to methicillin, by simple automated methodology, may soon become the standard for all microbiology laboratories servicing the critically ill. The second part of the study involved the development of a selective broth and multiplex PCR for detection of three important nosocomial isolates at this institution, methicillin-resistant S. aureus (MRSA), carbapenem-resistant Enterobacteriaceae, and multi-resistant Acinetobacter baumannii (MRAB). A multiplex PCR using four primer sets was designed to detect low colonisation levels of these isolates after overnight incubation in selective broth, significantly reducing the time to result and associated costs. This potentially useful epidemiological screening tool is practical, reproducible and sensitive with the potential of moving to an automated test (using real-time PCR, for example) in the future. The availability of early negative results judged by simple visual scanning (or by densitometry), means that the result is less operator-dependent, potentially reducing error rate. The last part of the study dealt with an important resistance phenotype, aminoglycoside resistance. There had been no recent comprehensive local surveys performed to determine the frequency of aminoglycoside resistance amongst the Enterobacteriaceae, or to identify the genetic determinants and their transmissibility. The isolates collected for the study were all resistant to at least one of gentamicin, tobramycin or amikacin. Identification of integron cassette arrays and use of specific internal primers identified at least one genetic determinant for gentamicin and tobramycin resistance in 22 of 23 isolates. Three isolates had two aminoglycoside resistance genes, and three isolates had three aminoglycoside resistance genes identified (Table 6.1). Transferable gentamicin-resistant plasmids were predominant amongst Klebsiella spp., but less so amongst Enterobacter spp. and E. coli. Gentamicin-resistant Klebsiella spp. were often ESBL positive, the genetic determinants of which were typically co-transferred on a conjugative plasmid. The importance of screening at a local level was demonstrated by the unexpected predominance of aac(6')-IIc amongst Enterobacter spp. and the detection of a new gene (aac(6')-LT). This part of the study has provided an understanding of the primary aminoglycoside resistance genes present in the local setting and their association with other resistances. This knowledge will allow development of assays for patient screening (clinical isolates and colonising flora), to better understand the epidemiology of aminoglycoside resistance and to allow better choice of antibiotic therapy related to presence or absence of these genes.
Robberts, Frans Jacob Lourens. "Genetic investigations of pneumocystis jirovecii : detection, cotrimoxazole resistance and population structure". Thesis, Link to the online version, 2005. http://hdl.handle.net/10019.1/1304.
Pełny tekst źródłaThomas, Lee Carolyn. "Genetic methods for rapid detection of medically important nosocomial bacteria". Connect to full text, 2007. http://hdl.handle.net/2123/3575.
Pełny tekst źródłaTitle from title screen (viewed 15 October 2008). Submitted in fulfilment of the requirements for the degree of Master of Science in Medicine to the Discipline of Medicine, Faculty of Medicine. Includes bibliographical references. Also available in print form.
Cohen, Joseph Paul. "Crater detection via genetic search methods to reduce image features". Thesis, University of Massachusetts Boston, 2014. http://pqdtopen.proquest.com/#viewpdf?dispub=1550596.
Pełny tekst źródłaRecent approaches to crater detection have been inspired by face detection's use of gray-scale texture features. Using gray-scale texture features for supervised machine learning crater detection algorithms provides better classification of craters in planetary images than previous methods. When using Haar features it is typical to generate thousands of numerical values from each candidate crater image. This magnitude of image features to extract and consider can spell disaster when the application is an entire planetary surface. One solution is to reduce the number of features extracted and considered in order to increase accuracy as well as speed. Feature subset selection provides the operational classifiers with a concise and denoised set of features by reducing irrelevant and redundant features. Feature subset selection is known to be NP-hard. To provide an efficient suboptimal solution, four genetic algorithms are proposed to use greedy selection, weighted random selection, and simulated annealing to distinguish discriminate features from indiscriminate features. Inspired by analysis regarding the relationship between subset size and accuracy, a squeezing algorithm is presented to shrink the genetic algorithm's chromosome cardinality during the genetic iterations. A significant increase in the classification performance of a Bayesian classifier in crater detection using image texture features is observed.
Konstantinidis, Michalis. "Preimplantation genetic diagnosis : new methods for the detection of genetic abnormalities in human preimplantation embryos". Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:28611f65-7729-4293-9c3f-4fc3f0cc39d7.
Pełny tekst źródłaUygun, Sahra. "Development Of Analysis Methods For Cry1ac And Sam-k Gene Lines In Tomato Using Pcr And Real-time Pcr". Master's thesis, METU, 2010. http://etd.lib.metu.edu.tr/upload/3/12611991/index.pdf.
Pełny tekst źródłaPecetti, Luciano. "Genetic resources and selection methods for drought and salinity resistance in durum wheat". Thesis, Bangor University, 1994. https://research.bangor.ac.uk/portal/en/theses/genetic-resources-and-selection-methods-for-drought-and-salinity-resistance-in-durum-wheat(119af68a-9751-4451-a54e-6c16fdb941ed).html.
Pełny tekst źródłaJurstrand, Margaretha. "Detection of Chlamydia trachomatis and Mycoplasma genitalium by genetic and serological methods". Doctoral thesis, Örebro : Universitetsbiblioteket : Örebro University, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-793.
Pełny tekst źródłaTreuerne, Balazs Krisztina Eszter. "Methods for the surveillance of urgent drug-resistant bacterial threats". Thesis, University of Sydney, 2020. https://hdl.handle.net/2123/24498.
Pełny tekst źródłaDufresne, Philippe J. "Development and validation of molecular markers for the detection of disease resistance alleles in Lactuca sativa". Thesis, McGill University, 2002. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=78352.
Pełny tekst źródłaHerrera-Foessel, Sybil A. "Enhancing the genetic diversity and durability of leaf rust resistance in durum wheat /". Uppsala : Dept. of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, 2007. http://epsilon.slu.se/200701.pdf.
Pełny tekst źródłaWong, Kam Cheung. "Intelligent methods of power system components monitoring by artificial neural networks and optimisation using evolutionary computing techniques". Thesis, University of Sunderland, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285580.
Pełny tekst źródłaHolmans, Peter Alan. "Maximum likelihood methods for the detection of genetic linkage and association using affected sibling pairs". Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320004.
Pełny tekst źródłaKokozidou, Maria. "Evaluation of PCR methods for detection, species identification and determination of genetic variation in L. infantum". [S.l.] : [s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=968411525.
Pełny tekst źródłaÖstholm, Balkhed Åse. "Extended-Spectrum ß-Lactamase-Producing Enterobacteriaceae : Antibiotic consumption, Detection and Resistance Epidemiology". Doctoral thesis, Linköpings universitet, Avdelningen för mikrobiologi och molekylär medicin, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-104216.
Pełny tekst źródłaCiampa, Julia Grant. "Multilocus approaches to the detection of disease susceptibility regions : methods and applications". Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:8f82a624-7d80-438c-af3e-68ce983ff45f.
Pełny tekst źródłaTom, Bryan Matthew. "A COMPARISON OF NONINVASIVE SURVEY METHODS FOR MONITORING MESOCARNIVORE POPULATIONS IN KENTUCKY". UKnowledge, 2012. http://uknowledge.uky.edu/forestry_etds/10.
Pełny tekst źródłaSargison, Neil Donald. "Development of genetic crossing methods to identify genes associated with macrocyclic lactone resistance in the sheep nematode parasite, Haemonchus contortus". Thesis, University of Edinburgh, 2009. http://hdl.handle.net/1842/4395.
Pełny tekst źródłaPreston, Jessica. "Overcoming the Current Limitations of Next-Generation Sequencing with New Methods for Local Assembly of Genomes and High-Specificity Rare Mutation Detection". Thesis, University of Oregon, 2016. http://hdl.handle.net/1794/19700.
Pełny tekst źródłaMontanari, Sara. "Identification and mapping of genomic regions controlling fire blight and psylla resistance and hybrid necrosis in pear". Thesis, Angers, 2015. http://www.theses.fr/2015ANGE0063/document.
Pełny tekst źródłaThe goal of this PhD project was to study the genetic architecture of pear resistance to two of its most significant diseases and pests, fire blight and psylla, which cause severe yield losses in all the main pear production regions worldwide. The development of new pear varieties with resistance against these two biotic stresses is of major interest for Integrated Pest Management. This project was designed in a joint collaboration among Fondazione Edmund Mach (Italy), Institut de Recherches en Horticulture et Semences (France) and Plant & Food Research (New-Zealand). The interspecific pear F1 progeny T003 x ‘Moonglow’ was developed with the purpose of cumulating resistances to fire blight and psylla deriving from Asian and European pear cultivars. Single nucleotide polymorphism (SNP) and simple sequence repeat (SSR)-based genetic maps were built for T003 and ‘Moonglow’. Quantitative Trait Loci (QTLs) were detected for the resistances, demonstrating their polygenic nature. Marker-assisted selection (MAS) can now be applied for these two traits. Furthermore, the segregating population exhibited genetic incompatibilities, and the genomic regions associated with hybrid necrosis were mapped for the first time in pear. Development of molecular markers linked to the lethal genes should allow breeders to avoid crosses leading to incompatible combinations that could affect the expression of important agronomic traits co-segregating with these genes
Sahin, Fikrettin. "Detection, identification and characterization of strains of Xanthomonas campestris pv. vesicatoria by traditional and molecular methods, and resistance in Capsicum species to Xanthomonas campestris pv. vesicatoria pepper race 6 /". The Ohio State University, 1997. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487945320758679.
Pełny tekst źródłaTibbo, Markos. "Productivity and health of indigenous sheep breeds and crossbreds in the central Ethiopian highlands /". Uppsala : Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, 2006. http://epsilon.slu.se/200651.pdf.
Pełny tekst źródłaSANTOS, Silas Garrido Teixeira de Carvalho. "Avaliação criteriosa dos algoritmos de detecção de concept drifts". Universidade Federal de Pernambuco, 2015. https://repositorio.ufpe.br/handle/123456789/17310.
Pełny tekst źródłaMade available in DSpace on 2016-07-11T12:33:28Z (GMT). No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) silas-dissertacao-versao-final-2016.pdf: 1708159 bytes, checksum: 6c0efc5f2f0b27c79306418c9de516f1 (MD5) Previous issue date: 2015-02-27
FACEPE
A extração de conhecimento em ambientes com fluxo contínuo de dados é uma atividade que vem crescendo progressivamente. Diversas são as situações que necessitam desse mecanismo, como o monitoramento do histórico de compras de clientes; a detecção de presença por meio de sensores; ou o monitoramento da temperatura da água. Desta maneira, os algoritmos utilizados para esse fim devem ser atualizados constantemente, buscando adaptar-se às novas instâncias e levando em consideração as restrições computacionais. Quando se trabalha em ambientes com fluxo contínuo de dados, em geral não é recomendável supor que sua distribuição permanecerá estacionária. Diversas mudanças podem ocorrer ao longo do tempo, desencadeando uma situação geralmente conhecida como mudança de conceito (concept drift). Neste trabalho foi realizado um estudo comparativo entre alguns dos principais métodos de detecção de mudanças: ADWIN, DDM, DOF, ECDD, EDDM, PL e STEPD. Para execução dos experimentos foram utilizadas bases artificiais – simulando mudanças abruptas, graduais rápidas, e graduais lentas – e também bases com problemas reais. Os resultados foram analisados baseando-se na precisão, tempo de execução, uso de memória, tempo médio de detecção das mudanças, e quantidade de falsos positivos e negativos. Já os parâmetros dos métodos foram definidos utilizando uma versão adaptada de um algoritmo genético. De acordo com os resultados do teste de Friedman juntamente com Nemenyi, em termos de precisão, DDM se mostrou o método mais eficiente com as bases utilizadas, sendo estatisticamente superior ao DOF e ECDD. Já EDDM foi o método mais rápido e também o mais econômico no uso da memória, sendo superior ao DOF, ECDD, PL e STEPD, em ambos os casos. Conclui-se então que métodos mais sensíveis às detecções de mudanças, e consequentemente mais propensos a alarmes falsos, obtêm melhores resultados quando comparados a métodos menos sensíveis e menos suscetíveis a alarmes falsos.
Knowledge extraction from data streams is an activity that has been progressively receiving an increased demand. Examples of such applications include monitoring purchase history of customers, movement data from sensors, or water temperatures. Thus, algorithms used for this purpose must be constantly updated, trying to adapt to new instances and taking into account computational constraints. When working in environments with a continuous flow of data, there is no guarantee that the distribution of the data will remain stationary. On the contrary, several changes may occur over time, triggering situations commonly known as concept drift. In this work we present a comparative study of some of the main drift detection methods: ADWIN, DDM, DOF, ECDD, EDDM, PL and STEPD. For the execution of the experiments, artificial datasets were used – simulating abrupt, fast gradual, and slow gradual changes – and also datasets with real problems. The results were analyzed based on the accuracy, runtime, memory usage, average time to change detection, and number of false positives and negatives. The parameters of methods were defined using an adapted version of a genetic algorithm. According to the Friedman test with Nemenyi results, in terms of accuracy, DDM was the most efficient method with the datasets used, and statistically superior to DOF and ECDD. EDDM was the fastest method and also the most economical in memory usage, being statistically superior to DOF, ECDD, PL and STEPD, in both cases. It was concluded that more sensitive change detection methods, and therefore more prone to false alarms, achieve better results when compared to less sensitive and less susceptible to false alarms methods.
Ferro, Isabelle Dangui. "Avaliação de diferentes métodos para a detecção e contagem de Campylobcter spp. isolado de carcaças de frango e determinação da prevalência e resistência antimicrobiana dos isolados = Evaluation of different methods for the detection and counting Campylobcter spp. isolated from chicken carcass in the state of Paraná and determination of the prevalence and antimicrobial resistance of isolated / Isabelle Dangui Ferro ; orientadora, Renata Ernlund Freitas de Macedo ; co-orientador Wanda Moscalewsli Abraão". reponame:Biblioteca Digital de Teses e Dissertações da PUC_PR, 2012. http://www.biblioteca.pucpr.br/tede/tde_busca/arquivo.php?codArquivo=2286.
Pełny tekst źródłaBibliografia: f. 102-107
Os produtos de origem animal são reconhecidos como fontes primárias de contaminação por vários enteropatógenos. Entre eles, nas últimas décadas, o gênero Campylobacter vem se destacando como o maior causador de surtos de gastrenterites humanas nos países
] The products of animal origin are recognized as primary sources of contamination by various pathogens. Among them the genus Campylobacter has emerged as the major cause of human gastroenteritis outbreaks in developed countries. Due to its importance in
Harrysson, Mattias. "Fault Location Algorithms in Transmission Grids". Thesis, Högskolan i Halmstad, Sektionen för ekonomi och teknik (SET), 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-26314.
Pełny tekst źródłaWeinstein, Zohar. "Substandard antimicrobial drugs: detection methods and their contributions to antibiotic resistance". Thesis, 2018. https://hdl.handle.net/2144/32951.
Pełny tekst źródła2020-10-24T00:00:00Z
Hsiao, Hsiangyu, i 蕭翔宇. "Development of molecular serotyping, detection methods and analysis of antibiotic resistance of Salmonella spp. in environmental water". Thesis, 2011. http://ndltd.ncl.edu.tw/handle/91370292514198624448.
Pełny tekst źródła國立中正大學
應用地球物理研究所
99
Salmonella spp. are widely distributed in the environment, which are found in livestock, wild animals and water. It can cause serious diseases in both human beings and animals, and infections with non-typhoidal Salmonella are a significant cause of illness and death worldwide.Furthermore, resistance to various classes of antimicrobial agents has been encountered in many bacteria of medical relevance. Particularly, attention has been paid to zoonotic bacteria such as Salmonella. In our study, we combined traditional culture method and molecular method to detect Salmonella in environmental water and to analyze its antimicrobial resistance. The aim of this study is to detect the occurrence of Salmonella from environmental water of Puzih stream and Kaupin River in Taiwan by polymerase chain reaction (PCR), then identify the serovar of Salmonella by PFGE and Multiplex PCR. At last, we use PCR and Disk Difussion Method to evaluate the antibiotic resistance of Salmonella. The occurrence frequency of Salmonella from Puzih stream was 21% (21/100) and Kaupin River was 40.35 % (23/57). The 44 positive water samples by culture method were further identified as S. Typhimurium (1/44), S. Bareilly (13/44), S. Isangi (3/44), S. Choleraesuis (2/44), S. Potsdam (1/44), S. Albany (10/44), S. Derby (4/44), S. Kedougou(3/44), S. Potsdam(1/44), S. Mbandaka(1/44), S. Oranienburg(1/44), S. Weltevreden(1/44), S. Agona(1/44) 以及 S. Newport (3/44)by biochemical testing, serological identification and PFGE. On the other hand, antibiotic resistance bacteria do exist in environmental water, and Salmonella spp. in environmental water also have the same trend of antibiotic resistance with clinical samples, including multidrug resistance Salmonella. Studies are needed to determine the sources of different multidrug-resistant Salmonella serotypes. Continued surveillance is needed to monitor changes in resistance trends and to detect further emergence of resistant Salmonella serotypes in Taiwan.
Sousa, Ana Catarina da Silva e. "Development of rapid methods for the detection of pathogenic microorganisms, based on NAM-FISH technology". Master's thesis, 2018. http://hdl.handle.net/10773/25528.
Pełny tekst źródłaO acesso de microrganismos patogénicos ao corpo humano pode comprometer a saúde do indivíduo, provocando variadas manifestações clínicas. Helicobacter pylori e Campylobacter são dois importantes patógenos gastrointestinais. A infeção por H. pylori é uma das infeções humanas mais comuns, cujo tratamento inclui a administração de antibióticos, nomeadamente fluoroquinolonas (FQ). Contudo, tem-se verificado um aumento da resistência de H. pylori a FQ, o que pode resultar em falhas no tratamento, tornando-se importante não só a deteção da bactéria, mas também a definição do seu perfil de resistência. Campylobacter é atualmente considerada a principal causa de doenças transmitidas por alimentos, encontrando-se normalmente associada ao consumo de carne crua. A deteção de microrganismos patogénicos pode ser alcançada por técnicas de cultura convencionais ou por métodos moleculares, nomeadamente testes imunológicos ou de deteção de ácidos nucleicos. A Biomode é uma empresa inovadora que desenvolve e comercializa métodos de diagnóstico rápidos baseados na tecnologia de hibridação fluorescente in situ (FISH) de ácidos nucleicos mímicos (NAM), NAM-FISH, que possibilita a deteção rápida de microrganismos, através da hibridação de sondas fluorescentes complementares com sequências específicas presentes no microrganismo alvo. Neste contexto, o presente trabalho teve como foco duas aplicações do NAM-FISH. Na área clínica, o objetivo principal foi o desenvolvimento de um método para a deteção de H. pylori e da resistência a FQ. Para tal, procedeu-se ao desenho de sondas de Peptide Nucleic Acid (PNA) e Locked Nucleic Acid (LNA)/2’OMe para a deteção de mutações causadoras da resistência. De forma a cobrir as mutações mais prevalentes, bem como o fenótipo wild-type, foram selecionadas 5 sondas de LNA/2’OMe. Na área da segurança alimentar, foi objetivo a otimização de um método de PNA-FISH para a deteção de Campylobacter em amostras alimentares. Um ensaio preliminar da inclusividade/exclusividade da sonda Campylobacter resultou na deteção de dois microrganismos não-alvo, H. cinaedi e H. pamatensis. Para a otimização do procedimento, foram utilizadas amostras de carne de frango crua, inoculadas artificialmente com C. jejuni. Antes do PNAFISH, foi introduzido um novo passo, no qual as amostras enriquecidas são sujeitas a uma centrifugação (10 000 g), seguida de ressuspensão em 0.1% Triton X-100, com o objetivo de reduzir a autofluorescência forte visualizada em amostras sem qualquer tratamento. Testou-se também a possibilidade de um enriquecimento das amostras em dois passos, no entanto, esta abordagem não demonstrou vantagens comparativamente ao procedimento em um passo. Efetuou-se ainda um teste de robustez, requerido pela AOAC International para a obtenção de certificação de produto, que revelou que a variação dos parâmetros do tempo e temperatura de hibridação influenciam a performance do método, pelo que as condições do PNA-FISH devem ser rigorosamente controladas. Os resultados obtidos neste estudo mostraram que o método PNAFISH é adequado para a rápida deteção de Campylobacter em amostras alimentares. Com este trabalho conclui-se que, embora os dois métodos baseados em NAM-FISH sejam uma opção promissora para a deteção de H. pylori e Campylobacter, ambos necessitam de otimização futura.
Mestrado em Biotecnologia
Kokozidou, Maria [Verfasser]. "Evaluation of PCR methods for detection, species identification and determination of genetic variation in L. infantum / submitted by Maria Kokozidou". 2003. http://d-nb.info/968411525/34.
Pełny tekst źródłaYang, Man-Hsia, i 楊滿霞. "Statistical Methods for Genome-wide Detection of QTL Hotspots toward Understanding the Complex Genetic Architecture of Quantitative Traits Using Public Databases with Application to Rice". Thesis, 2018. http://ndltd.ncl.edu.tw/handle/7782ff.
Pełny tekst źródła國立臺灣大學
農藝學研究所
107
Genome-wide detection of quantitative trait loci (QTL) hotspots underlying variation in many molecular and phenotypic traits has been a key step in various biological studies since the QTL hotspots are highly informative and can be linked to the genes for the quantitative traits. Several statistical methods have been proposed to detect QTL hotspots. These hotspot detection methods rely heavily on permutation tests performed on summarized QTL data or individual-level data (with genotypes and phenotypes) from the genetical genomics experiments. In this article, I proposed a statistical procedure for QTL hotspot detection by using the summarized QTL (interval) data collected in public web-accessible databases. First, a simple statistical method based on the uniform distribution is derived to convert the QTL interval data into the expected QTL frequency (EQF) matrix. And then, to account for the correlation structure among traits, the QTLs for correlated traits are grouped together into the same categories to form a reduced EQF matrix. Furthermore, a permutation algorithm on the EQF elements or on the QTL intervals is developed to compute a sliding scale of EQF thresholds, ranging from strict to liberal, for assessing the significance of QTL hotspots. With grouping, much stricter thresholds can be obtained to avoid the detection of spurious hotspots. Real example analysis and simulation study were carried out to illustrate our procedure, evaluate the performances and compare with other methods. It showed that our procedure can control the genome-wide error rates at the target levels, provide appropriate thresholds for correlated data and be comparable to the methods using individual-level data in hotspot detection. Depending on the thresholds used, more than 100 hotspots are detected in GRAMENE rice database. I also performed a genome-wide comparative analysis of the detected hotspots and the known genes collected in the Rice Q-TARO database. The comparative analysis revealed that the hotspots and genes were conformable in the sense that they co-localize closely and were functionally related to relevant traits. Our statistical procedure can provide a framework for exploring the networks among QTL hotspots, genes and quantitative traits in biological studies. The R package QHOT that produce both numerical and graphical outputs of QTL hotspot detection in the genome are available on the worldwide web http://www.stat.sinica.edu.tw/chkao/ and has been submitted to Comprehensive R Archive Network (CRAN).
"Optimising aspects of a soybean breeding programme". Thesis, 2008. http://hdl.handle.net/10413/738.
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