Gotowe bibliografie tematyczne / Gene technology

Gotowa bibliografia na temat „Gene technology”

Autor: Grafiati
Data publikacji: 4 czerwca 2021
Data aktualizacji: 20 lutego 2023

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Artykuły w czasopismach na temat "Gene technology"

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Ezzell, Carol. "For gene technology". Nature 336, nr 6198 (grudzień 1988): 500–501. http://dx.doi.org/10.1038/336500a0.

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SCHIBECI, RENATO, i IAN BARNS. "Gene Technology Communication". Science Communication 20, nr 2 (grudzień 1998): 204–26. http://dx.doi.org/10.1177/1075547098020002003.

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Ekker, Stephen C., i Jon D. Larson. "Morphant technology in model developmental systems". genesis 30, nr 3 (2001): 89–93. http://dx.doi.org/10.1002/gene.1038.

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Perrons, Robert K., i Lew Watts. "The Selfish Technology Gene". Journal of Petroleum Technology 60, nr 04 (1.04.2008): 20–22. http://dx.doi.org/10.2118/0408-0020-jpt.

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Zinkernagel, R. M. "Gene Technology and Democracy". Science 278, nr 5341 (14.11.1997): 1207. http://dx.doi.org/10.1126/science.278.5341.1207.

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Cavalli, Franco. "Gene Technology and Democracy". Science 279, nr 5348 (9.01.1998): 155.4–155. http://dx.doi.org/10.1126/science.279.5348.155d.

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Ban, Elizabeth. "Australian gene technology survey". Nature Medicine 1, nr 9 (wrzesień 1995): 857. http://dx.doi.org/10.1038/nm0995-857b.

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Doi, Nobuhide, i Hiroshi Yanagawa. "Insertional gene fusion technology". FEBS Letters 457, nr 1 (20.08.1999): 1–4. http://dx.doi.org/10.1016/s0014-5793(99)00991-6.

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Gershon, Diane. "Tools for gene technology". Nature 349, nr 6307 (styczeń 1991): 353–54. http://dx.doi.org/10.1038/349353a0.

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Prosser, Jane. "Gene technology for all". Trends in Biotechnology 13, nr 8 (sierpień 1995): 312. http://dx.doi.org/10.1016/s0167-7799(00)88973-8.

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Rozprawy doktorskie na temat "Gene technology"

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Busby, Michele Anne. "Measuring Gene Expression With Next Generation Sequencing Technology". Thesis, Boston College, 2012. http://hdl.handle.net/2345/3145.

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Thesis advisor: Gabor Marth
While a PhD student in Dr. Gabor Marth's laboratory, I have had primary responsibility for two projects focused on using RNA-Seq to measure differential gene expression. In the first project we used RNA-Seq to identify differentially expressed genes in four yeast species and I analyzed the findings in terms of the evolution of gene expression. In this experiment, gene expression was measured using two biological replicates of each species of yeast. While we had several interesting biological findings, during the analysis we dealt with several statistical issues that were caused by the experiment's low number of replicates. The cost of sequencing has decreased rapidly since this experiment's design and many of these statistical issues can now practically be avoided by sequencing a greater number of samples. However, there is little guidance in the literature as to how to intelligently design an RNA-Seq experiment in terms of the number of replicates that are required and how deeply each replicate must be sequenced. My second project, therefore, was to develop Scotty, a web-based program that allows users to perform power analysis for RNA-Seq experiments. The yeast project resulted in a highly accessed first author publication in BMC Genomics in 2011. I have structured my dissertation as follows: The first chapter, entitled General Issues in RNA-Seq, is intended to synthesize the themes and issues of RNA-Seq statistical analysis that were common to both papers. In this section, I have discussed the main findings from the two papers as they relate to analyzing RNA-Seq data. Like the Scotty application, this section is designed to be "used" by wet-lab biologists who have a limited background in statistics. While some background in statistics would be required to fully understand the following chapters, the essence of this background can be gained by reading this first chapter. The second and third chapters contain the two papers that resulted from the two RNA-Seq projects. Each chapter contains both the original manuscript and original supplementary methods and data section. Finally, I include brief summaries of my contributions to the two papers on which I was a middle author. The first was a functional analysis of the genomic regions affected by mobile element insertions as a part of Chip Stewart's paper with the 1000 Genome Consortium. This paper was published in Plos Genetics. The second was a cluster analysis of microarray gene expression in Toxoplasma gondii, which was included as part of Alexander Lorestani et al.'s paper, Targeted proteomic dissection of Toxoplasma cytoskeleton sub-compartments using MORN1. This paper is currently under review. The yeast project was a collaborative effort between Jesse Gray, Michael Springer, and Allen Costa at Harvard Medical School, Jeffery Chuang here at Boston College, and members of the Marth lab. Jesse Gray conceived of the project. While I wrote the draft for the manuscript, many people, particularly Gabor Marth, provided substantial guidance on the actual text. I conceived of and implemented Scotty and wrote its manuscript with only editorial assistance from my co-authors. I produced all figures for the two manuscripts. Chip Stewart provided extensive guidance and mentorship to me on all aspects of my statistical analyses for both projects
Thesis (PhD) — Boston College, 2012
Submitted to: Boston College. Graduate School of Arts and Sciences
Discipline: Biology
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Heywood, Jacqualine, i n/a. "'Talking' and 'doing' gene technology politics: a policy analysis". Griffith University. Australian School of Environmental Studies, 2004. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20041029.100010.

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This thesis explores the environmental politics surrounding agricultural biotechnology innovations and diffusion. Recent developments in agricultural biotechnology are accompanied by growing social concerns that such innovations pose risks to the environment and to human health. Biosafety is a term used to discuss the possibility of such risks. Currently, the regulation of agricultural gene-technology and biosafety are contentious environmental issues for national and international policy communities. However, detailed studies of the conflicts and complexities generated by biotechnology for environmental governance are scarce. In particular, little is understood of the ways in which biotechnology issues emerge on regulatory agendas, and research gaps remain on how differing perspectives of biotechnological risks impact on policy outcomes. This thesis makes a significant contribution to these outstanding research issues. My contribution is a new analytical framework that unearths the discursive role biotechnology plays in constructing international environmental policy regimes. I develop this framework on the understanding that the use of language resources like storylines, metaphors and other rhetorical devices are critical in shaping environmental policy in general and biotechnology governance in particular. This analytical framework couples a language analysis to an investigation of the practices of institutional power. The result is a discourse analysis that provides important and useful insights into the theory and practice of biosafety policy. In other words, my thesis explores both the ‘talking’ and the ‘doing’ of policymaking and thereby provides new insights into the contested and uncertain environmental policy area of international gene-technology regulation. Specifically, I undertake a discourse analysis of international biosafety politics within the Convention on Biological Diversity. I apply my discourse analysis to a case study: the Cartagena Protocol on Biosafety to the Convention on Biological Diversity, 2000. My research provides a different reading of international gene-technology politics, one that questions the constructed nature of biotechnology as a policy problem and reveals the power relations involved in producing particular policy options and outcomes on biosafety. There are a number of key research findings that emerged from the application of my discursive analytical framework to the Cartagena Protocol on Biosafety. I find that biosafety is a highly fluid concept. It can enlarge or contract depending on the way in which language resources are mobilised by policy actors and interest groups to secure definitions and generate consensus around their preferred understandings of biosafety. Moreover, my research indicates that the more radical texts for biosafety can be recast by dominant interest groups into scripts for shallow reform agendas. Institutionalised policy practices also effect policy outcomes. My research finds that the use of Expert Panels, for example, is important in shaping international policy communities’ understanding of the policy problems posed by biotechnology risks. In the light of these findings, my thesis argues that the ability of interest groups and policy actors to win language games within institutional settings also enables them to secure their preferred policy outcomes. I import the concept of authorship as a new policy concept to discuss the ways in which such groups exercise social power to secure their understanding of biosafety, which thereby effect the ‘writing’ of the dominant accounts of what constitutes an acceptable international biosafety standard within the Cartagena Protocol. In short, my thesis is a new account of biosafety politics that fills some of the current knowledge gaps about how biotechnology is emerging onto regulatory agendas. It also demonstrates the mechanisms of power and the language struggles that determine biosafety policy outcomes within multi-lateral environmental agreements such as the Convention on Biological Diversity.
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Naujoks, Daniel. "Developing a gene targeting technology for Anopheles gambiae mosquitoes". Thesis, Imperial College London, 2012. http://hdl.handle.net/10044/1/9864.

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Studying gene function in the human malaria vector Anopheles gambiae is the key to understanding its biology and vector-parasite interactions. Existing tools to study gene function do not include gene targeting, which would allow insights into gene function by permitting a range of specific modifications to any gene of choice. Based on developments in Drosophila (RONG and GOLIC 2000) this PhD project proposes to establish a method for gene targeting in Anopheles by homologous recombination, using a linear targeting construct generated in vivo. To this end, a transgenic strain was generated that expresses FLP recombinase and I-SceI endonuclease under the control of the germline-specific vasa promoter. Together they were to excise and linearise a targeting molecule from a transgenic “donor” locus. Homology in the targeting construct would enable integration, via recombination in the germline, at a gene of interest, thereby permitting its targeted modification. Expression of vasa-driven I-SceI resulted in high cleavage activity in the germline. A systematic analysis using a variety of transgenic target loci revealed that homologous repair, rather than non-homologous end joining, was the predominant mechanism employed to repair the double stranded breaks generated by I-SceI. These findings offer encouraging prospects for population genetic engineering using homing endonuclease genes (BURT 2003). FLP activity was shown in Anopheles cell culture, yet in vivo excision via FLP at two independent loci in the germline was not observed, precluding the obtainment of a knock-out. After eliminating many possible sources of error, likely causes include antagonistic interference between I-SceI and FLP or unfavourable reaction kinetics of FLP. Examination of the ortholog of Drosophila yellow-g1 as a target gene suggests it is required somatically for female fertility, making it a good candidate for vector population control with a genetic drive system based on homing endonuclease genes. Furthermore, the above finding that recombination is promoted by endonuclease activity driven by the vasa promoter augurs well for its use in mediating efficient drive in such a system.
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Heywood, Jacqualine. "'Talking' and 'doing' gene technology politics: a policy analysis". Thesis, Griffith University, 2004. http://hdl.handle.net/10072/365762.

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This thesis explores the environmental politics surrounding agricultural biotechnology innovations and diffusion. Recent developments in agricultural biotechnology are accompanied by growing social concerns that such innovations pose risks to the environment and to human health. Biosafety is a term used to discuss the possibility of such risks. Currently, the regulation of agricultural gene-technology and biosafety are contentious environmental issues for national and international policy communities. However, detailed studies of the conflicts and complexities generated by biotechnology for environmental governance are scarce. In particular, little is understood of the ways in which biotechnology issues emerge on regulatory agendas, and research gaps remain on how differing perspectives of biotechnological risks impact on policy outcomes. This thesis makes a significant contribution to these outstanding research issues. My contribution is a new analytical framework that unearths the discursive role biotechnology plays in constructing international environmental policy regimes. I develop this framework on the understanding that the use of language resources like storylines, metaphors and other rhetorical devices are critical in shaping environmental policy in general and biotechnology governance in particular. This analytical framework couples a language analysis to an investigation of the practices of institutional power. The result is a discourse analysis that provides important and useful insights into the theory and practice of biosafety policy. In other words, my thesis explores both the ‘talking’ and the ‘doing’ of policymaking and thereby provides new insights into the contested and uncertain environmental policy area of international gene-technology regulation. Specifically, I undertake a discourse analysis of international biosafety politics within the Convention on Biological Diversity. I apply my discourse analysis to a case study: the Cartagena Protocol on Biosafety to the Convention on Biological Diversity, 2000. My research provides a different reading of international gene-technology politics, one that questions the constructed nature of biotechnology as a policy problem and reveals the power relations involved in producing particular policy options and outcomes on biosafety. There are a number of key research findings that emerged from the application of my discursive analytical framework to the Cartagena Protocol on Biosafety. I find that biosafety is a highly fluid concept. It can enlarge or contract depending on the way in which language resources are mobilised by policy actors and interest groups to secure definitions and generate consensus around their preferred understandings of biosafety. Moreover, my research indicates that the more radical texts for biosafety can be recast by dominant interest groups into scripts for shallow reform agendas. Institutionalised policy practices also effect policy outcomes. My research finds that the use of Expert Panels, for example, is important in shaping international policy communities’ understanding of the policy problems posed by biotechnology risks. In the light of these findings, my thesis argues that the ability of interest groups and policy actors to win language games within institutional settings also enables them to secure their preferred policy outcomes. I import the concept of authorship as a new policy concept to discuss the ways in which such groups exercise social power to secure their understanding of biosafety, which thereby effect the ‘writing’ of the dominant accounts of what constitutes an acceptable international biosafety standard within the Cartagena Protocol. In short, my thesis is a new account of biosafety politics that fills some of the current knowledge gaps about how biotechnology is emerging onto regulatory agendas. It also demonstrates the mechanisms of power and the language struggles that determine biosafety policy outcomes within multi-lateral environmental agreements such as the Convention on Biological Diversity.
Thesis (PhD Doctorate)
Doctor of Philosophy (PhD)
Australian School of Environmental Studies
Full Text
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Fält, Susann. "Analysis of global gene expression in complex biological systems using microarray technology /". Stockholm, 2006. http://diss.kib.ki.se/2006/91-7140-612-3/.

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He, Huiqi. "Miniaturized electroporation system for gene transfer using bio-MEMS technology /". View abstract or full-text, 2007. http://library.ust.hk/cgi/db/thesis.pl?BIEN%202007%20HE.

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White, Adam. "Microfluidic technology for high-throughput single cell gene expression analysis". Thesis, University of British Columbia, 2010. http://hdl.handle.net/2429/27838.

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Transcription measurements with single cell resolution are critical to understanding variable responses in immunity, measuring stochastic noise in gene expression, and assessing the disease and developmental state of heterogeneous populations. The latter is particularly important in stem cell science, developmental biology, and cancer, where minority cells may be most significant. To see these populations requires the quick and cost-effective measurement of hundreds to thousands of individual cells. Quantitative real-time polymerase chain reaction (RT-qPCR) is a sensitive method for quantitative analysis of transcript levels that provides excellent sensitivity and dynamic range in the detection of transcripts. However, the use of RT-qPCR is generally limited to ensemble measurements of bulk cells or plasma, and is blind to minority cell populations. This aggregation obscures the underlying biological response and variability. To address this limitation, we exploit recent advances in scalable microfluidics to develop robust lab-on-chip technology capable of highly parallel and cost-effective measurements of transcript levels from single cells. The microfluidic device integrates single-cell capture, lysis, reverse transcription of contained RNA, and precise measurement of cDNA using RT-qPCR. We demonstrate this system in the study of microRNA expression in a cell line representing chronic myelogenous leaukemia, pluripotency markers in differentiating human embryonic stem cells, and the detection of somatic mutations in a primary breast cancer sample. The ability to screen isolated cells by simultaneously measuring the fraction of cells expressing a specific gene and quantifying the abundance of expression, may provide a new modality for the early detection of disease such as cancer.
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Kotnik, Katarina [Verfasser]. "Gene knockdown in transgenic rats by shRNA technology / Katarina Kotnik". Berlin : Freie Universität Berlin, 2008. http://d-nb.info/1023169665/34.

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Catteruccia, Flaminia. "Systematic attempts to develop gene transfer technology for anopheline mosquitoes". Thesis, Imperial College London, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323867.

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Alete, Julia. "Safe, site-specific gene delivery using ultrasound and microbubble technology". Thesis, Imperial College London, 2008. http://hdl.handle.net/10044/1/4256.

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The following study investigates the use of diagnostic ultrasound in combination with microbubbles (ultrasound contrast agents) as a physical enhancer for non-viral gene delivery. The aim of this work was firstly, to demonstrate that ultrasound exposure using settings within the range of diagnostic ultrasound, in combination with microbubbles can improve gene delivery, and secondly, to show that it is a safe, site-specific technique which mitigates the risk of tissue damage often seen with other physical enhancers of gene delivery such as, electroporation. Initially, a feasibility study was carried out to test the efficiency and safety of microbubble ultrasound (MBUS) in a reporter gene setting. Experiments using intravenous injections of a luciferase reporter gene established that MBUS is a safe, site-specific technique which improved levels of the luciferase expression in the organ targeted by MBUS. Luciferase was successfully delivered to the liver and heart, showing significantly higher levels compared to injections without MBUS, and with no detectable expression in other non-target organs. A therapeutic application of MBUS was tested using the mdx mouse, an animal model for Duchenne Muscular Dystrophy (DMD), a genetic disorder caused by the lack of functional dystrophin in muscle fibres due to premature termination of translation. The most successful treatment approach in the mdx mouse thus far had been the injection of Phosphorodiamidate Morpholino Oligomers (PMOs), which by inducing exon skipping, re-introduced dystrophin expression in most muscles in the body, with the exception of the heart. Injections of PMOs with MBUS to the heart successfully re-introduced dystrophin expression in cardiomyocytes. Furthermore, treatment parameters were investigated in more detail in order to optimize PMO delivery to the heart. Finally, an investigation into different types of commercially available microbubbles compared the efficiencies (with respect to gene delivery) of the different bubbles, in order to understand why different microbubbles show different results when used for MBUS, potentially enabling the design of microbubbles specifically for gene delivery.
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Książki na temat "Gene technology"

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Jarvis, Sue. Gene technology. Christchurch, N.Z: NZ Institute for Crop & Food Research Ltd., 1995.

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Zander, Axel R., Wolfram Ostertag, Boris V. Afanasiev i Frank Grosveld, red. Gene Technology. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-61122-3.

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Fiechter, Armin, i Christof Sautter, red. Green Gene Technology. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71323-4.

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Parliament, Tasmania. Report on gene technology. [Hobart, Tas: Government Printer], 2001.

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Susanne, Lundin, i Åkesson Lynn, red. Gene technology and economy. Lund: Nordic Academic Press, 2002.

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Kahl, Günter. Dictionary of gene technology. Weinheim: VCH, 1995.

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Winnacker, Ernst-L. From genes to clones: Introduction to gene technology. Weinheim: VCH, 1987.

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Winnacker, Ernst-L. From genes to clones: Introduction to gene technology. Weinheim: VCH Verlagsgesellschaft, 1987.

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Winnacker, Ernst L. From genes to clones: Introduction to gene technology. Weinheim, Federal Republic of Germany: VCH, 1987.

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Gene technology: Confronting the issues. New York: F. Watts, 1990.

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Części książek na temat "Gene technology"

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Mitsuhashi, Jun. "Gene Technology". W Invertebrate Tissue Culture Methods, 379–86. Tokyo: Springer Japan, 2002. http://dx.doi.org/10.1007/978-4-431-67875-5_41.

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McBride, Lincoln, Ken Livak, Mike Lucero, Federico Goodsaid, Dane Carlson, Junko Stevens, Traci Allen i in. "Quantitative PCR Technology". W Gene Quantification, 97–110. Boston, MA: Birkhäuser Boston, 1998. http://dx.doi.org/10.1007/978-1-4612-4164-5_6.

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Hole, Nicholas, Roland Leung, Lobat Doostdar, Ursula Menzel, Kay Samuel, Janice Murray, Helen Taylor, Gerry Grahaml i John Ansell. "Hematopoietic Differentiation of Embryonal Stem Cells in vitro." W Gene Technology, 3–10. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-61122-3_1.

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Spritz, Richard A. "Molecular Basis of Genetic Disorders of Pigmentation in Humans and Mice". W Gene Technology, 145–74. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-61122-3_10.

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Stuif, I., A. Sominskaya, T. Bykova, O. Frolova i A. Zaritskey. "MDR-1 Gene Expression in Chronic Myelogenous Leukemia: Prognostic Marker of the Disease". W Gene Technology, 175–79. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-61122-3_11.

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Gerrard, Ann. "Towards Gene Therapy for Haemophilia B". W Gene Technology, 183–88. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-61122-3_12.

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Hatzfeld, Jacques, Béatrice Panterne, Pascal Batard, Patricia Sansilvestri, Ma-Lin Li, Angelo A. Cardoso i Antoinette Hatzfeld. "Inhibition of autocrine TGF-ß in CD34+ human progenitor cells reveals their potentiality to engraft adults and improves gene transfer efficiency". W Gene Technology, 189–92. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-61122-3_13.

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Heslop, Helen E., Cliona M. Rooney, Donna R. Rill i Malcolm K. Brenner. "Gene Transfer into Hemopoietic Progenitors: Implications for Bone Marrow Transplantation". W Gene Technology, 193–202. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-61122-3_14.

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Just, Ursula, Norbert Ahlers, Gökhan Arman, Nicholas Hunt, Wolfram Ostertag i Joachim Nowock. "Erythropoietin and the ENV gp55 of the Spleen Focus Forming Virus (SFFV) Interact Differently with Erythroid Cells in the Mouse and in Tissue Culture". W Gene Technology, 203–18. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-61122-3_15.

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Baum, Christopher, Hans-Georg Eckert i Wolfram Ostertag. "Contribution of the Retroviral Leader to Gene Transfer and Expression in Packaging Cells and Myeloid Stem and Progenitor Cells". W Gene Technology, 219–28. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-61122-3_16.

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Streszczenia konferencji na temat "Gene technology"

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Gumerova, G., A. Chemeris i B. Kuluev. "Biolistic transformation of plants using CRISPR/Cas9 technology". W 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.098.

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Experiments on CRISPR/Cas9 mediated knock-in approaches in the PDS gene of various genes of interest were planned. Biolistic bombardment mediated delivery of target vectors to plant explants was suggested.
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Kjeldaas, S., i T. Antonsen. "49. Visions of gene technology". W EurSafe 2021. The Netherlands: Wageningen Academic Publishers, 2021. http://dx.doi.org/10.3920/978-90-8686-915-2_49.

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Hazrina Yusof Hamdani i Siti Rohkmah Mohd Shukri. "Gene prediction system". W 2008 International Symposium on Information Technology. IEEE, 2008. http://dx.doi.org/10.1109/itsim.2008.4631728.

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Li, Ling. "CRISPR/Cas9-based editing of OsNF-YC4/GmNF-YC4 promoter yields high-protein crops". W 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/qsgt8379.

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Genome editing is a new breeding technology widely touted for transgene-free crop improvement; however, to date, the majority of derived traits are created through gene knockout. We describe a novel approach using gene editing to upregulate gene expression by removing negative repressor binding motifs. Our previous work demonstrated that ectopic expression of NF-YC4 increases protein content of leaves and seeds at the expense of carbohydrates. We detected several conserved motifs predicted to be bound by repressors in the promoter of rice and soybean NF-YC4 genes. Using CRISPR/Cas9 to edit the promoters of rice and soybean NF-YC4 genes, we deleted promoter fragments harboring repressor binding motifs. Those deletions resulted in decreased repressor binding, increased NF-YC4 expression, increased protein and decreased carbohydrates. Gene-edited plants showed up to 48% higher leaf protein and 15% increased seed protein. Moreover, we exemplify a general approach for upregulating gene expression through targeted genomic deletions.
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Robbins, Jeff. "Technology and the Gene for Minimizing Effort". W 2006 IEEE International Symposium on Technology and Society. IEEE, 2006. http://dx.doi.org/10.1109/istas.2006.4375895.

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Qian, Zhao, Zhang Yan i Lin Zhengkui. "Mining of Gene Modules and Identification of Key Genes in Hepatocellular Carcinoma based on Gene Co-expression Network Analysis". W ICBBT 2020: 2020 12th International Conference on Bioinformatics and Biomedical Technology. New York, NY, USA: ACM, 2020. http://dx.doi.org/10.1145/3405758.3405762.

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Babichev, S., V. Lytvynenko, J. Skvor, M. Korobchynskyi i M. Voronenko. "Information Technology of Gene Expression Profiles Processing for Purpose of Gene Regulatory Networks Reconstruction". W 2018 IEEE Second International Conference on Data Stream Mining & Processing (DSMP). IEEE, 2018. http://dx.doi.org/10.1109/dsmp.2018.8478452.

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Qofrani, Elnaz, Mehrdad Jalali i Mohamad Reza Kalani. "The improvement of accuracy of gene expression data classification with gene ontology". W 2014 International Congress on Technology, Communication and Knowledge (ICTCK). IEEE, 2014. http://dx.doi.org/10.1109/ictck.2014.7033532.

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Deng, Xiaozheng, i Yu Chen. "Inference of Gene Regulations Between Multiple Activators/Inhibitors and Singular Genes". W 2018 9th International Conference on Information Technology in Medicine and Education (ITME). IEEE, 2018. http://dx.doi.org/10.1109/itme.2018.00051.

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Ananth, Alaka, i K. Chandra Sekaran. "Service optimization in cloud using family gene technology". W 2014 International Conference on Advances in Computing, Communications and Informatics (ICACCI). IEEE, 2014. http://dx.doi.org/10.1109/icacci.2014.6968491.

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Raporty organizacyjne na temat "Gene technology"

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Marcelo Bento Soares. Technology development for gene discovery and full-length sequencing. Office of Scientific and Technical Information (OSTI), lipiec 2004. http://dx.doi.org/10.2172/825961.

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Conklin, Douglas S. Use of Novel Stable Gene Silencing Technology to Determine the Contribution of the Receptor. Fort Belvoir, VA: Defense Technical Information Center, październik 2003. http://dx.doi.org/10.21236/ada419821.

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Young, Erin, Cem Kuscu, Christine Watkins i Murat Dogan. Using CRISPR Gene Editing to Prevent Accumulation of Lipids in Hepatocytes. University of Tennessee Health Science Center, styczeń 2022. http://dx.doi.org/10.21007/com.lsp.2022.0007.

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CRISPR gene editing is a molecular technology that can be used to silence gene expression. In this experiment, genes that are known to play a role in lipid accumulation in hepatocytes were targeted. Specifically, levels of fatty acid transport proteins 2 and 5 (FATP2 & 5) have been shown to be elevated in cases of non-alcoholic fatty liver disease. The goal of this experiment was to reduce expression of these genes by using a dead Cas9 (dCas9) protein with an attached inhibitory domain (KRAB) that acts on the promotor region. When measuring the mRNA expression, it was determined that the levels of the CRISPR-modified gene products were significantly reduced compared to the control. However, the same extent of inhibition was not consistently observed when conducting flow cytometry. Current work is aimed at discovering why lipid accumulation is not inhibited to the expected degree based on the results of mRNA expression.
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Lerman, L. S. [Cloning-idependent mapping technology for genomic fidelity, contig linking, C-DNA site analysis, and gene detection]. Final report. Office of Scientific and Technical Information (OSTI), grudzień 1994. http://dx.doi.org/10.2172/10111141.

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Reisch, Bruce, Avichai Perl, Julie Kikkert, Ruth Ben-Arie i Rachel Gollop. Use of Anti-Fungal Gene Synergisms for Improved Foliar and Fruit Disease Tolerance in Transgenic Grapes. United States Department of Agriculture, sierpień 2002. http://dx.doi.org/10.32747/2002.7575292.bard.

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Original objectives . 1. Test anti-fungal gene products for activity against Uncinula necator, Aspergillus niger, Rhizopus stolonifer and Botrytis cinerea. 2. For Agrobacterium transformation, design appropriate vectors with gene combinations. 3. Use biolistic bombardment and Agrobacterium for transformation of important cultivars. 4. Characterize gene expression in transformants, as well as level of powdery mildew and Botrytis resistance in foliage of transformed plants. Background The production of new grape cultivars by conventional breeding is a complex and time-consuming process. Transferring individual traits via single genes into elite cultivars was proposed as a viable strategy, especially for vegetatively propagated crops such as grapevines. The availability of effective genetic transformation procedures, the existence of genes able to reduce pathogen stress, and improved in vitro culture methods for grapes, were combined to serve the objective of this proposal. Effective deployment of resistance genes would reduce production costs and increase crop quality, and several such genes and combinations were used in this project. Progress The efficacy of two-way combinations of Trichoderma endochitinase (CHIT42), synthetic peptide ESF12 and resveratrol upon the control of growth of Botrytis cinerea and Penicillium digitatum were evaluated in vitro. All pairwise interactions were additive but not synergistic. Per objective 2, suitable vectors with important gene combinations for Agrobacterium transformation were designed. In addition, multiple gene co-transformation by particle bombardment was also tested successfully. In New York, transformation work focused on cultivars Chardonnay and Merlot, while the technology in Israel was extended to 41B, R. 110, Prime, Italia, Gamay, Chardonnay and Velika. Transgenic plant production is summarized in the appendix. Among plants developed in Israel, endochitinase expression was assayed via the MuchT assay using material just 1-5 days after co-cultivation. Plants of cv. Sugraone carrying the gene coding for ESF12, a short anti-fungal lytic peptide under the control of the double 358 promoter, were produced. Leaf extracts of two plants showed inhibition zones that developed within 48 h indicating the inhibitory effect of the leaf extracts on the six species of bacteria. X fastidiosa, the causal organism of Pierce's disease, was very sensitive to leaf extracts from ESF12 transformed plants. Further work is needed to verify the agricultural utility of ESF12 transformants. In New York, some transformants were resistant to powdery mildew and Botrytis fruit rot. Major conclusions, solutions, achievements and implications The following scientific achievements resulted from this cooperative BARD project: 1. Development and improvement of embryogenesis and tissue culture manipulation in grape, while extending these procedures to several agriculturally important cultivars both in Israel and USA. 2. Development and improvement of novel transformation procedures while developing transformation techniques for grape and other recalcitrant species. 3. Production of transgenic grapevines, characterization of transformed vines while studying the expression patterns of a marker gene under the control of different promoter as the 35S CaMV in different part of the plants including flowers and fruits. 4. Expression of anti-fungal genes in grape: establishment of transgenic plants and evaluation of gene expression. Development of techniques to insert multiple genes. 5. Isolation of novel grape specific promoter to control the expression of future antimicrobial genes. It is of great importance to report that significant progress was made in not only the development of transgenic grapevines, but also in the evaluation of their potential for increased resistance to disease as compared with the non engineered cultivar. In several cases, increased disease resistance was observed. More research and development is still needed before a product can be commercialized, yet our project lays a framework for further investigations.
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Marton, L. Exploration of new perspectives and limitations in Agrobacterium-mediated gene transfer technology. Final report, June 1, 1992--May 31, 1995. Office of Scientific and Technical Information (OSTI), luty 1996. http://dx.doi.org/10.2172/184286.

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Marton, L. Exploration of new perspectives and limitations in Agrobacterium mediated gene transfer technology. Progress report, [June 1, 1992-- May 31, 1994]. Office of Scientific and Technical Information (OSTI), grudzień 1994. http://dx.doi.org/10.2172/197759.

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Savaldi-Goldstein, Sigal, i Todd C. Mockler. Precise Mapping of Growth Hormone Effects by Cell-Specific Gene Activation Response. United States Department of Agriculture, grudzień 2012. http://dx.doi.org/10.32747/2012.7699849.bard.

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Plant yield largely depends on a complex interplay and feedback mechanisms of distinct hormonal pathways. Over the past decade great progress has been made in elucidating the global molecular mechanisms by which each hormone is produced and perceived. However, our knowledge of how interactions between hormonal pathways are spatially and temporally regulated remains rudimentary. For example, we have demonstrated that although the BR receptor BRI1 is widely expressed, the perception of BRs in epidermal cells is sufficient to control whole-organ growth. Supported by additional recent works, it is apparent that hormones are acting in selected cells of the plant body to regulate organ growth, and furthermore, that local cell-cell communication is an important mechanism. In this proposal our goals were to identify the global profile of translated genes in response to BR stimulation and depletion in specific tissues in Arabidopsis; determine the spatio-temporal dependency of BR response on auxin transport and signaling and construct an interactive public website that will provide an integrated analysis of the data set. Our technology incorporated cell-specific polysome isolation and sequencing using the Solexa technology. In the first aim, we generated and confirmed the specificity of novel transgenic lines expressing tagged ribosomal protein in various cell types in the Arabidopsis primary root. We next crossed these lines to lines with targeted expression of BRI1 in the bri1 background. All lines were treated with BRs for two time points. The RNA-seq of their corresponding immunopurified polysomal RNA is nearly completed and the bioinformatic analysis of the data set will be completed this year. Followed, we will construct an interactive public website (our third aim). In the second aim we started revealing how spatio-temporalBR activity impinges on auxin transport in the Arabidopsis primary root. We discovered the unexpected role of BRs in controlling the expression of specific auxin efflux carriers, post-transcriptionally (Hacham et al, 2012). We also showed that this regulation depends on the specific expression of BRI1 in the epidermis. This complex and long term effect of BRs on auxin transport led us to focus on high resolution analysis of the BR signaling per se. Taking together, our ongoing collaboration and synergistic expertise (hormone action and plant development (IL) and whole-genome scale data analysis (US)) enabled the establishment of a powerful system that will tell us how distinct cell types respond to local and systemic BR signal. BR research is of special agriculture importance since BR application and BR genetic modification have been shown to significantly increase crop yield and to play an important role in plant thermotolerance. Hence, our integrated dataset is valuable for improving crop traits without unwanted impairment of unrelated pathways, for example, establishing semi-dwarf stature to allow increased yield in high planting density, inducing erect leaves for better light capture and consequent biomass increase and plant resistance to abiotic stresses.
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Conklin, Douglas S. Use of a Novel, Stable Gene Silencing Technology to Determine the Contribution of the Receptor Tyrosine Kinase to the Breast Cancer Phenotype. Fort Belvoir, VA: Defense Technical Information Center, październik 2004. http://dx.doi.org/10.21236/ada435144.

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Wilson, Thomas E., Avraham A. Levy i Tzvi Tzfira. Controlling Early Stages of DNA Repair for Gene-targeting Enhancement in Plants. United States Department of Agriculture, marzec 2012. http://dx.doi.org/10.32747/2012.7697124.bard.

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Gene targeting (GT) is a much needed technology as a tool for plant research and for the precise engineering of crop species. Recent advances in this field have shown that the presence of a DNA double-strand break (DSB) in a genomic locus is critical for the integration of an exogenous DNA molecule introduced into this locus. This integration can occur via either non-homologous end joining (NHEJ) into the break or homologous recombination (HR) between the broken genomic DNA and the introduced vector. A bottleneck for DNA integration via HR is the machinery responsible for homology search and strand invasion. Important proteins in this pathway are Rad51, Rad52 and Rad54. We proposed to combine our respective expertise: on the US side, in the design of zincfinger nucleases (ZFNs) for the induction of DNA DSBs at any desired genomic locus and in the integration of DNA molecules via NHEJ; and on the Israeli side in the HR events, downstream of the DSB, that lead to homology search and strand invasion. We sought to test three major pathways of targeted DNA integration: (i) integration by NHEJ into DSBs induced at desired sites by specially designed ZFNs; (ii) integration into DSBs induced at desired sites combined with the use of Rad51, Rad52 and Rad54 proteins to maximize the chances for efficient and precise HR-mediated vector insertion; (iii) stimulation of HR by Rad51, Rad52 and Rad54 in the absence of DSB induction. We also proposed to study the formation of dsT-DNA molecules during the transformation of plant cells. dsT-DNA molecules are an important substrate for HR and NHEJ-mediatedGT, yet the mode of their formation from single stranded T-DNA molecules is still obscure. In addition we sought to develop a system for assembly of multi-transgene binary vectors by using ZFNs. The latter may facilitate the production of binary vectors that may be ready for genome editing in transgenic plants. ZFNs were proposed for the induction of DSBs in genomic targets, namely, the FtsH2 gene whose loss of function can easily be identified in somatic tissues as white sectors, and the Cruciferin locus whose targeting by a GFP or RFP reporter vectors can give rise to fluorescent seeds. ZFNs were also proposed for the induction of DSBs in artificial targets and for assembly of multi-gene vectors. We finally sought to address two important cell types in terms of relevance to plant transformation, namely GT of germinal (egg) cells by floral dipping, and GT in somatic cells by root and leave transformation. To be successful, we made use of novel optimized expression cassettes that enable coexpression of all of the genes of interest (ZFNs and Rad genes) in the right tissues (egg or root cells) at the right time, namely when the GT vector is delivered into the cells. Methods were proposed for investigating the complementation of T-strands to dsDNA molecules in living plant cells. During the course of this research, we (i) designed, assembled and tested, in vitro, a pair of new ZFNs capable of targeting the Cruciferin gene, (ii) produced transgenic plants which expresses for ZFN monomers for targeting of the FtsH2 gene. Expression of these enzymes is controlled by constitutive or heat shock induced promoters, (iii) produced a large population of transgenic Arabidopsis lines in which mutated mGUS gene was incorporated into different genomic locations, (iv) designed a system for egg-cell-specific expression of ZFNs and RAD genes and initiate GT experiments, (v) demonstrated that we can achieve NHEJ-mediated gene replacement in plant cells (vi) developed a system for ZFN and homing endonuclease-mediated assembly of multigene plant transformation vectors and (vii) explored the mechanism of dsTDNA formation in plant cells. This work has substantially advanced our understanding of the mechanisms of DNA integration into plants and furthered the development of important new tools for GT in plants.
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