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Pearl, Christopher A. "Demonstration of pheromonal activity in the breeding glands of dwarf African clawed frogs (Hymenochirus sp.)". Scholarly Commons, 2000. https://scholarlycommons.pacific.edu/uop_etds/529.
Pełny tekst źródłaMadison, Amanda L. "Effects of male breeding gland in hymenochirus on female reproductive output". Scholarly Commons, 2005. https://scholarlycommons.pacific.edu/uop_etds/610.
Pełny tekst źródłaMcDearmid, Jonathan R. "Noradrenergic control of spinal motor circuitry in two related amphibian species, Xenopus laevis and Rama temporaria". Thesis, University of St Andrews, 1998. http://hdl.handle.net/10023/15058.
Pełny tekst źródłaPascarelli, Erica S. "Behavioral evidence for pheromonal communication : female discrimination of androgen status in male Xenopus laevis"". Scholarly Commons, 1995. https://scholarlycommons.pacific.edu/uop_etds/2289.
Pełny tekst źródłaWord, James Mabry. "Physiological adjustments to aestivation and activity in the cocoon-forming frogs Cyclorana platycephala and Cyclorana maini". University of Western Australia. School of Animal Biology, 2008. http://theses.library.uwa.edu.au/adt-WU2008.0254.
Pełny tekst źródłaAllen, Leon Akila Glynne. "Effect of temperature on the physiology of two exotic frogs: possible causes of distribution". Thesis, University of Canterbury. Biological Sciences, 2015. http://hdl.handle.net/10092/10822.
Pełny tekst źródłaPearl, Christopher A. "Demonstration of pheromonal activity in the breeding glands of dwarf African clawed frogs (Hymenochirus sp.) : a thesis". Scholarly Commons, 2001. https://scholarlycommons.pacific.edu/uop_etds/529.
Pełny tekst źródłaWang, Yu. "HPLC method development for the evaluation of pheromones from the dwarf African clawed frog Hymenochirus". Scholarly Commons, 2003. https://scholarlycommons.pacific.edu/uop_etds/587.
Pełny tekst źródłaContreras, Heidy Lorena. "Effects of natural history on osmoregulatory behaviors in two stream-dwelling frogs (Pseudacris cadaverina and P. regilla)". CSUSB ScholarWorks, 2007. https://scholarworks.lib.csusb.edu/etd-project/3253.
Pełny tekst źródłaWang, Yu. "HPLC method development for the evaluation of pheromones from the dwarf African clawed frog Hymenochirus : a thesis". Scholarly Commons, 2001. https://scholarlycommons.pacific.edu/uop_etds/587.
Pełny tekst źródłaSadowski, Leslie M. "The influence of lipids in the epidermis and cocoons on cutaneous water loss in Australian hylid frogs, Cyclorana spp". The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1273708738.
Pełny tekst źródłaBuckler, K. J. "Actions of adrenergic agonists on transmembrane ion exchanges in skeletal and heart muscle". Thesis, University of Newcastle Upon Tyne, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.380754.
Pełny tekst źródłaMason, M. J. "Mechanisms of entry of L-lactate into frog skeletal muscle : A micro-electrode study". Thesis, University of Bristol, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375026.
Pełny tekst źródłaCrawford, Niall Alexander. "The biomechanics of tree frog adhesion under challenging conditions". Thesis, University of Glasgow, 2016. http://theses.gla.ac.uk/7102/.
Pełny tekst źródłaBeecher, Nancy A. "Frog declines exploring connections among climate change, immunity and disease /". [Bloomington, Ind.] : Indiana University, 2006. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3243772.
Pełny tekst źródłaTitle from PDF t.p. (viewed Nov. 18, 2008). Source: Dissertation Abstracts International, Volume: 67-12, Section: B, page: 6837. Advisers: Greg E. Demas; Craig Nelson.
Gramolini, Anthony Orlando. "The effect of modulating ATP-sensitive potassium channels in frog skeletal muscle, in vitro, during fatigue and metabolic inhibition". Thesis, University of Ottawa (Canada), 1996. http://hdl.handle.net/10393/9473.
Pełny tekst źródłaChou, Kuang-Yi. "Sodium channel regulatory mechanisms : current fluctuation analysis on frog skin epithelium". Doctoral thesis, University of Cape Town, 1994. http://hdl.handle.net/11427/27148.
Pełny tekst źródłado, Amaral Maria Clara Figueirinhas. "Geographical variation of freeze tolerance in the wood frog, Rana sylvatica: the role of hepatic glycogen metabolism". Miami University / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=miami1406900958.
Pełny tekst źródłaBethany, Williams. "Nitric oxide metabolites in hypoxia, freezing, and hibernation of the wood frog, Rana sylvatica". Miami University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=miami1525202133341325.
Pełny tekst źródłaSandusky, Paula. "The biomechanics of tongue projection in the frog _Rana pipiens_: dynamics and temperature effects". Scholar Commons, 2012. http://scholarcommons.usf.edu/etd/4219.
Pełny tekst źródłaRosendale, Andrew J. "Characterization and physiological regulation of glucose transporter 2 in the liver of the wood frog, Rana sylvatica: implications for freeze tolerance". Miami University / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=miami1403172987.
Pełny tekst źródłaParker, Mark. "Independent Acoustic Stimulation of the Amphibian and Basilar Papillae of Rana pipiens". PDXScholar, 1995. https://pdxscholar.library.pdx.edu/open_access_etds/4956.
Pełny tekst źródła"Molecular cloning of growth hormone and growth hormone receptor in lower vertebrates". 2000. http://library.cuhk.edu.hk/record=b5895794.
Pełny tekst źródłaThesis (M.Phil.)--Chinese University of Hong Kong, 2000.
Includes bibliographical references (leaves 148-155).
Abstracts in English and Chinese.
Abstract --- p.i
摘要 --- p.iii
Acknowledgments --- p.v
Contents --- p.vi
List of figures --- p.xii
List of table --- p.xiv
Abbreviations --- p.xv
Chapter Chapter 1 --- General Introduction
Chapter 1.1. --- Growth hormone (GH) --- p.1
Chapter 1.1.1. --- Introduction to GH --- p.1
Chapter 1.1.2. --- Actions of GH --- p.2
Chapter 1.1.3. --- Structure of GH --- p.3
Chapter 1.1.4. --- The sequence of GH --- p.5
Chapter 1.2. --- Growth hormone receptor (GHR) --- p.6
Chapter 1.2.1 --- Introduction to GHR --- p.6
Chapter 1.2.2. --- Structure of the extracellular domain of GHR --- p.9
Chapter 1.2.3. --- The regulation of GHR --- p.12
Chapter 1.2.4. --- GHR biosynthesis --- p.13
Chapter 1.2.5. --- Tissue distribution of GHR --- p.14
Chapter 1.3. --- Signal transduction mechanisms of GHR --- p.15
Chapter 1.3.1. --- Dimerization of GH and GHR complex --- p.15
Chapter 1.3.2. --- The Jak and Stat pathway --- p.18
Chapter 1.3.3. --- The ras and other signaling pathways --- p.20
Chapter 1.4. --- Project aim --- p.22
Chapter Chapter 2 --- Material and Methods
Chapter 2.1. --- Preparation of ribonuclease free reagents and apparatus --- p.23
Chapter 2.2. --- Isolation of total RNA --- p.23
Chapter 2.3. --- Isolation of mRNA --- p.24
Chapter 2.4. --- Spectrophotometric quantification and qualification of DNA and RNA --- p.24
Chapter 2.5. --- First strand cDNA synthesis --- p.25
Chapter 2.6. --- Agarose gel electrophoresis of DNA --- p.25
Chapter 2.7. --- Formaldehyde agarose gel electrophoresis of RNA --- p.26
Chapter 2.8. --- Vacuum transfer of DNA to a nylon membrane --- p.26
Chapter 2.9. --- Nucleic acids purification by MicroSpin (S-200HR) columns --- p.27
Chapter 2.10. --- DNA radioactive labeling by nick translation --- p.27
Chapter 2.11. --- Southern blot analysis --- p.28
Chapter 2.12. --- Autoradiography and molecular imager --- p.28
Chapter 2.13 . --- Linearization and dephosphorylation of plasmid DNA --- p.29
Chapter 2.14. --- Purification of DNA from agarose using QIAEX II kit --- p.29
Chapter 2.15. --- 3'End modification of PCR amplified DNA --- p.30
Chapter 2.16. --- Ligation of DNA fragments to linearized vector --- p.30
Chapter 2.17. --- Preparation of Escherichia coli competent cells --- p.31
Chapter 2.18. --- Transformation --- p.31
Chapter 2.19. --- Mini preparation of plasmid DNA --- p.32
Chapter 2.20. --- Maxi preparation of plasmid DNA --- p.34
Chapter 2.21 . --- PCR sequencing --- p.35
Chapter 2.22. --- cDNA library screening --- p.36
Chapter 2.23. --- Preparation and sterilization of culture medium --- p.38
Chapter 2.24. --- Preparation of frozen stock of culture cells --- p.39
Chapter 2.25. --- Cell passage of CHO-Kl --- p.39
Chapter 2.26. --- Counting of cells --- p.40
Chapter 2.27. --- Proliferation assay performed on CHO-K1 cells (MTT method) --- p.40
Chapter 2.28. --- Luciferase assay --- p.41
Chapter 2.29. --- SDS-PAGE preparation --- p.42
Chapter 2.30. --- SDS-PAGE analysis of proteins --- p.42
Chapter 2.31 . --- Recombinant protein expression --- p.43
Chapter 2.32. --- Small scale purification of recombinant proteins --- p.44
Chapter 2.33. --- Restriction digestion of DNA --- p.45
Chapter 2.34. --- Purification of PCR product using QIAquick PCR purification kit --- p.45
Chapter 2.35. --- TA cloning of PCR fragment --- p.45
Chapter 2.36. --- Transfection of plasmid into CHO-K1 cells --- p.46
Chapter 2.37. --- Sources of hormones --- p.46
Chapter 2.38. --- Buffer and reagents --- p.47
Chapter Chapter 3 --- "Cloning, expression and tissue distribution of Xenopus laevis GHR"
Chapter 3.1. --- Introduction --- p.50
Chapter 3.2. --- Materials and methods --- p.51
Chapter 3.2.1. --- Molecular cloning of xGHR cDNA
Chapter 3.2.1.1. --- Animals and tissues --- p.51
Chapter 3.2.1.2. --- Reverse transcribed´ؤpolymerase chain reaction (RT-PCR) --- p.51
Chapter 3.2.1.3. --- Subcloning of PCR amplified DNA fragment --- p.53
Chapter 3.2.1.4. --- Library screening of xGHR --- p.53
Chapter 3.2.1.5. --- 5 'Rapid amplification of cDNA end (5' RACE) --- p.55
Chapter 3.2.2. --- Tissue distribution of xGHR
Chapter 3.2.2.1. --- Animals and tissues --- p.56
Chapter 3.2.2.2. --- RT-PCR and Southern blot --- p.56
Chapter 3.2.3. --- Eukarytoic expression of xGHR and functional assay of xGHR
Chapter 3.2.3.1. --- Subcloning ofxGHR into pRc/CMV --- p.57
Chapter 3.2.3.2. --- Expression of xGHR in CHO-K1 cell --- p.58
Chapter 3.2.3.3. --- Proliferation assay --- p.58
Chapter 3.3. --- Results --- p.60
Chapter 3.3.1. --- RT-PCR of the partial fragment --- p.60
Chapter 3.3.2. --- Library screening of xGHR cDNA library --- p.61
Chapter 3.3.3. --- 5' RACE --- p.64
Chapter 3.3.4. --- The full-length cDNA sequence of xGHR --- p.65
Chapter 3.3.5. --- Tissue distribution of xGHR mRNA --- p.69
Chapter 3.3.6. --- Functional assay of xGHR in CHO-K1 cells --- p.71
Chapter 3.4. --- Discussion --- p.74
Chapter Chapter 4 --- Cloning and expression of Xenopus laevis GH-A and GH-B
Chapter 4.1. --- Introduction --- p.78
Chapter 4.2. --- Materials and Methods --- p.79
Chapter 4.2.1. --- PCR amplification of xGH-A and xGH-B partial fragments --- p.79
Chapter 4.2.2. --- cDNA library screening of xGH-A and xGH-B --- p.80
Chapter 4.2.3. --- Rapid amplification of cDNA ends of xGH-B
Chapter 4.2.3.1. --- 3'RACE --- p.80
Chapter 4.2.3.2. --- 5'RACE --- p.81
Chapter 4.2.4. --- Expression of xGH-A and xGH-B
Chapter 4.2.4.1 --- Construction of the expression vector --- p.84
Chapter 4.2.4.2. --- Protein expression of xGH-A and xGH-B --- p.85
Chapter 4.2.5. --- Purification of recombinant xGH-A and xGH-B --- p.85
Chapter 4.3. --- Results --- p.87
Chapter 4.3.1. --- PCRof xGH-A and xGH-B partial fragment --- p.87
Chapter 4.3.2. --- Library screening of xGH-A --- p.87
Chapter 4.3.3. --- 5' RACE and 3' RACE of xGH-B --- p.91
Chapter 4.3.4. --- Sequence analysis of xGH-A and xGH-B --- p.93
Chapter 4.3.5. --- Protein expression and purification of recombinant xGH-A and xGH-B --- p.100
Chapter 4.4. --- Discussion --- p.102
Chapter Chapter 5 --- Molecular cloning and function expression of goldfish GHR
Chapter 5.1. --- Introduction --- p.105
Chapter 5.2. --- Materials and methods --- p.106
Chapter 5.2.1. --- Molecular cloning of the partial fragment of gfGHR
Chapter 5.2.1.1. --- Primer design --- p.106
Chapter 5.2.1.2. --- Library PCR of gfGHR partial fragment --- p.108
Chapter 5.2.2. --- Library PCR of gfGHR cDNA sequence --- p.110
Chapter 5.2.3. --- Determination of 3' End and 5' End sequences of gfGHR cDNA --- p.112
Chapter 5.2.4. --- Tissue distribution of gfGHR
Chapter 5.2.4.1. --- Animals and tissues --- p.115
Chapter 5.2.4.2. --- Semi-quantitative R T-PCR --- p.115
Chapter 5.2.5. --- Functional expression of gfGHR in CHO-K1 cell
Chapter 5.2.5.1. --- Construction of an expression vector containing gfGHR --- p.116
Chapter 5.2.5.2. --- Functional assay of gfGHR expression on CHO-K1 cells --- p.117
Chapter 5.2.5.3. --- Proliferation assay --- p.118
Chapter 5.2.5.4. --- Spi luciferase assay --- p.118
Chapter 5.3. --- Results --- p.120
Chapter 5.3.1. --- PCR amplification of the partial sequence of gfGHR --- p.120
Chapter 5.3.2. --- The library PCR of gfGHR cDNA sequence --- p.122
Chapter 5.3.3. --- The sequence of gfGHR --- p.124
Chapter 5.3.4. --- Tissue distribution of gfGHR --- p.131
Chapter 5.3.5. --- Proliferation assay --- p.133
Chapter 5.3.6. --- Spi luciferase assay --- p.135
Chapter 5.4. --- Discussion --- p.137
Chapter Chapter 6 --- General discussion and future works --- p.145
References --- p.148
Appendix --- p.156
Yang, Sungchil. "Intrinsic biophysical properties of frog central auditory neurons /". 2008. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3337970.
Pełny tekst źródłaSource: Dissertation Abstracts International, Volume: 69-11, Section: B, page: 6623. Adviser: Albert S. Feng. Includes bibliographical references. Available on microfilm from Pro Quest Information and Learning.
Li, Zheng. "GABAergic organization in the visual system of the leopard frog, Rana pipiens". 1996. https://scholarworks.umass.edu/dissertations/AAI9619407.
Pełny tekst źródłaKralian, Susan M. "Stabilization of the frog neuromuscular junction: Terminal Schwann cells and the actin cytoskeleton". 2003. https://scholarworks.umass.edu/dissertations/AAI3110513.
Pełny tekst źródłaChen, Te-Chih, i 陳德治. "A comparative study of the reproduction and tadpole thermal physiology between the cold water and hot spring populations of Japanese buerger''s frog (Buergeria japonica)". Thesis, 1999. http://ndltd.ncl.edu.tw/handle/31063760894171992530.
Pełny tekst źródła"Interleukin-1(beta) as a potential biomarker of methylmercury exposure in developing neural circuits of the frog, Xenopus laevis". Tulane University, 1998.
Znajdź pełny tekst źródłaacase@tulane.edu