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Artykuły w czasopismach na temat „Fluorescent Bioimaging”

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Data publikacji: 7 września 2023

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1

ZHU, MING-QIANG, GUO-FENG ZHANG, CHONG LI, YA-JING LI, MATTHEW P. ALDRED i ALEXANDER D. Q. LI. "PHOTOSWITCHABLE NANOFLUOROPHORES FOR INNOVATIVE BIOIMAGING". Journal of Innovative Optical Health Sciences 04, nr 04 (październik 2011): 395–408. http://dx.doi.org/10.1142/s1793545811001423.

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Photosensitive fluorescent probes have become powerful tools in chemical biology and molecular biophysics, which are used to investigate cellular processes with high temporal and spatial resolution. Accordingly, photosensitive fluorescent probes, including photoactivatable, photoconvertible, and photoswitchable fluorophores, have been extensively developed during the past decade. The photoswitchable fluorophores have received much attention because they highlight cellular events clearly. This minireview summarizes recent advances of using reversibly photoswitchable fluorophores and their applications in innovative bioimaging. Photoswitchable fluorophores include photoswitchable fluorescent proteins, photoswitchable fluorescent organic molecules (dyes), and photoswitchable fluorescent nanoparticles. Several strategies have been developed to synthesize photoswitchable fluorophores, including engineering combination proteins, chemical synthesis, polymerization, and self-assembly. Here we concentrate on polymer nanoparticles with optically switchable emission properties: either fluorescence on/off or dual-alternating-color fluorescence photoswitching. The essential mechanisms of fluorescence photoswitching enable different types of photoswitchable fluorophores to change emission intensity or wavelength (color) and thus validating the basis of the fluorescence on/off or dual-color photoswitching design. Generally the possible applications of any fluorophores are to label biological targets, followed by specific imaging. The newly developed photoswitchable fluorophores enable super-resolution fluorescence imaging because of their photosensitive emission. Finally, we summarize the important area regarding future research and development on photoswitchable fluorescent nanoparticles.

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Gedara, Sriyani Menike Korale, Zi-You Ding, Iresha Lakmali Balasooriya, Yingchao Han i Merita Nirmali Wickramaratne. "Hydrothermal Synthesis and In Vivo Fluorescent Bioimaging Application of Eu3+/Gd3+ Co-Doped Fluoroapatite Nanocrystals". Journal of Functional Biomaterials 13, nr 3 (29.07.2022): 108. http://dx.doi.org/10.3390/jfb13030108.

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In this study, Eu3+/Gd3+ co-doped fluoroapatitååe (Eu/Gd:FAP) nanocrystals were synthesized by the hydrothermal method as a fluorescent bioimaging agent. The phase composition, morphology, fluorescence, and biosafety of the resulting samples were characterized. Moreover, the in vivo fluorescent bioimaging application of Eu/Gd:FAP nanocrystals was evaluated in mice with subcutaneously transplanted tumors. The results showed that the Eu/Gd:FAP nanocrystals were short rod-like particles with a size of 59.27 ± 13.34 nm × 18.69 ± 3.32 nm. With an increasing F substitution content, the Eu/Gd:FAP nanocrystals displayed a decreased size and enhanced fluorescence emission. Eu/Gd:FAP nanocrystals did not show hemolysis and cytotoxicity, indicating good biocompatibility. In vivo fluorescent bioimaging study demonstrated that Eu/Gd:FAP nanocrystals could be used as a bioimaging agent and displayed stable fluorescence emitting in tumors, indicating an accumulation in tumor tissue due to the passive targeting ability. In addition, any adverse effects of Eu/Gd:FAP nanocrystals on major organs were not observed. This study shows that biocompatible rare earth co-doped FAP nanocrystals have the potential to be used as a bioimaging agent in vivo.

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Wang, Xinyue, Dandan Sang, Liangrui Zou, Shunhao Ge, Yu Yao, Jianchao Fan i Qinglin Wang. "Multiple Bioimaging Applications Based on the Excellent Properties of Nanodiamond: A Review". Molecules 28, nr 10 (12.05.2023): 4063. http://dx.doi.org/10.3390/molecules28104063.

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Nanodiamonds (NDs) are emerging as a promising candidate for multimodal bioimaging on account of their optical and spectroscopic properties. NDs are extensively utilized for bioimaging probes due to their defects and admixtures in their crystal lattice. There are many optically active defects presented in NDs called color centers, which are highly photostable, extremely sensitive to bioimaging, and capable of electron leap in the forbidden band; further, they absorb or emit light when leaping, enabling the nanodiamond to fluoresce. Fluorescent imaging plays a significant role in bioscience research, but traditional fluorescent dyes have some drawbacks in physical, optical and toxicity aspects. As a novel fluorescent labeling tool, NDs have become the focus of research in the field of biomarkers in recent years because of their various irreplaceable advantages. This review primarily focuses on the recent application progress of nanodiamonds in the field of bioimaging. In this paper, we will summarize the progress of ND research from the following aspects (including fluorescence imaging, Raman imaging, X-ray imaging, magnetic modulation fluorescence imaging, magnetic resonance imaging, cathodoluminescence imaging, and optical coherence tomography imaging) and expect to supply an outlook contribution for future nanodiamond exploration in bioimaging.

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Yang, Yufei, Fucheng Gao, Yandong Wang, Hui Li, Jie Zhang, Zhiwei Sun i Yanyan Jiang. "Fluorescent Organic Small Molecule Probes for Bioimaging and Detection Applications". Molecules 27, nr 23 (1.12.2022): 8421. http://dx.doi.org/10.3390/molecules27238421.

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The activity levels of key substances (metal ions, reactive oxygen species, reactive nitrogen, biological small molecules, etc.) in organisms are closely related to intracellular redox reactions, disease occurrence and treatment, as well as drug absorption and distribution. Fluorescence imaging technology provides a visual tool for medicine, showing great potential in the fields of molecular biology, cellular immunology and oncology. In recent years, organic fluorescent probes have attracted much attention in the bioanalytical field. Among various organic fluorescent probes, fluorescent organic small molecule probes (FOSMPs) have become a research hotspot due to their excellent physicochemical properties, such as good photostability, high spatial and temporal resolution, as well as excellent biocompatibility. FOSMPs have proved to be suitable for in vivo bioimaging and detection. On the basis of the introduction of several primary fluorescence mechanisms, the latest progress of FOSMPs in the applications of bioimaging and detection is comprehensively reviewed. Following this, the preparation and application of fluorescent organic nanoparticles (FONPs) that are designed with FOSMPs as fluorophores are overviewed. Additionally, the prospects of FOSMPs in bioimaging and detection are discussed.

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5

Luo, Xiaofeng, Jiaqi Meng, Baolin Li, Aidong Peng i Zhiyuan Tian. "Development of fluorescent nanoparticles with aggregation-induced delayed fluorescence features, improved brightness and photostability for living cells imaging". New Journal of Chemistry 43, nr 27 (2019): 10735–43. http://dx.doi.org/10.1039/c9nj01945f.

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Lin, Zhong, Hong Wang, Maolin Yu, Xiang Guo, Chonghua Zhang, Haitao Deng, Peisheng Zhang i in. "Photoswitchable ultrahigh-brightness red fluorescent polymeric nanoparticles for information encryption, anti-counterfeiting and bioimaging". Journal of Materials Chemistry C 7, nr 37 (2019): 11515–21. http://dx.doi.org/10.1039/c9tc04054d.

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Péresse, Tiphaine, i Arnaud Gautier. "Next-Generation Fluorogen-Based Reporters and Biosensors for Advanced Bioimaging". International Journal of Molecular Sciences 20, nr 24 (5.12.2019): 6142. http://dx.doi.org/10.3390/ijms20246142.

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Our ability to observe biochemical events with high spatial and temporal resolution is essential for understanding the functioning of living systems. Intrinsically fluorescent proteins such as the green fluorescent protein (GFP) have revolutionized the way biologists study cells and organisms. The fluorescence toolbox has been recently extended with new fluorescent reporters composed of a genetically encoded tag that binds endogenously present or exogenously applied fluorogenic chromophores (so-called fluorogens) and activates their fluorescence. This review presents the toolbox of fluorogen-based reporters and biosensors available to biologists. Various applications are detailed to illustrate the possible uses and opportunities offered by this new generation of fluorescent probes and sensors for advanced bioimaging.

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8

Song, Chenxi, Shubiao Zhang, Quan Zhou, Hua Hai, Defeng Zhao i Yunze Hui. "Upconversion nanoparticles for bioimaging". Nanotechnology Reviews 6, nr 2 (1.04.2017): 233–42. http://dx.doi.org/10.1515/ntrev-2016-0043.

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AbstractFluorescent labeling is a widely used indispensable tool in biology. Conventional downconversion fluorescence labels with ultraviolet or short-wavelength excitation suffer from autofluorescence, low signal-to-noise ratio, and incident photo damage to living organisms. However, upconverting fluorescent nanoparticles emit detectable photons of higher energy in the near-infrared (NIR) or visible range upon irradiation with a NIR light in a process termed upconversion. They overcome some of the disadvantages faced by conventional downconversion labels with the advantages including very low autofluorescence, absence of photo damage to living organisms, high detection sensitivity, and high depth of light penetration, thus making them an ideal fluorescent label for bioimaging. The present review focuses on the features of upconversion nanoparticles, the applications of upconversion nanoparticles in bioimaging, and the bioimaging equipments and methods and discusses the obstacles and development trend of upconversion nanoparticles in bioimaging; we hope this work will provide insights into the study of relevant fields. Upconversion nanoparticles have special photoluminescent properties. Substantial advancements have been made in the field of upconversion nanoparticles for bioimaging. A large number of modifications of upconversion nanoparticles are studied to make them more hydrophilic and biocompatible. At the same time, the safety and toxicity of nanoparticles have caused wide public concern.

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9

Terai, Takuya, i Tetsuo Nagano. "Fluorescent probes for bioimaging applications". Current Opinion in Chemical Biology 12, nr 5 (październik 2008): 515–21. http://dx.doi.org/10.1016/j.cbpa.2008.08.007.

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Pu, Kan-Yi, i Bin Liu. "Fluorescent Conjugated Polyelectrolytes for Bioimaging". Advanced Functional Materials 21, nr 18 (15.08.2011): 3408–23. http://dx.doi.org/10.1002/adfm.201101153.

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Fryer, Claudia, Patricia Murray i Haifei Zhang. "Evaluation of Cytotoxicity and Bioimaging of Nitrogen-Vacancy Nanodiamonds". Nanomaterials 12, nr 23 (25.11.2022): 4196. http://dx.doi.org/10.3390/nano12234196.

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Nanodiamonds, due to their chemical inertness and biocompatibility, have found extensive uses in drug delivery and biomedical applications. Fluorescent nanodiamonds with fluorescent properties generated by nitrogen-vacancy defects have been intensively investigated for bioimaging, due to their high quantum yield and high photobleaching stability. In addition, the surface properties and particle size of nanodiamonds have significant impacts on cellular uptake and imaging quality. In this study, nitrogen-vacancy nanodiamonds with different particle sizes (40 nm and 90 nm) have been physicochemically characterised and investigated for their cytotoxicity and potential in fluorescence imaging. The nanodiamonds (with concentrations up to 100 µg/mL) showed cell viability >70% with mesenchymal stromal cells. The number of nanodiamonds was observed to have a larger impact on cell viability than the mass of nanodiamonds. Larger nanodiamonds (90 nm) exhibited a lower level of cytotoxicity, higher cellular uptake and fluorescence intensity. The results indicate the potential of using fluorescent nanodiamonds as a nanoprobe for effective bioimaging and cell tracking.

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Lu, Yanjiao, Bicheng He, Zhuo Gao, Jie Li, Jie Shen, Wantai Yang i Meizhen Yin. "One-Pot Synthesis of Cy5-Encapsulated Photostable Fluorescent Silica Nanoparticles for Bioimaging". Nano LIFE 05, nr 03 (wrzesień 2015): 1540007. http://dx.doi.org/10.1142/s1793984415400073.

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A new type of Cy5-encapsulated photostable fluorescent silica nanoparticles (FSNPs) bearing positive charges have been successfully fabricated by a reverse microemulsion synthesis in one-pot. The Cy5 dye containing four primary amines are embedded into silica via covalent bonds through a silane coupling agent (GPTMS), followed by co-condensation with tetraethylorthosilicate. The uniform-sized, spherical and monodispersed FSNPs have high fluorescence intensity and photostability. The FSNPs exhibit high stability, good biocompatibility as well as low cytotoxicity. These FSNPs can be internalized into live cells and thus fluorescently label the cells. This study provides a simple synthesis approach that can be applied to other water-soluble and amino-modified organic dye molecules for biological targeting and fluorescent cell imaging.

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13

Sun, Zhiyong, Yangxue Li, Xingang Guan, Tingting Sun, Li Chen, Zhigang Xie i Xiabin Jing. "A single-step emulsion approach to prepare fluorescent nanoscale coordination polymers for bioimaging". RSC Adv. 4, nr 28 (2014): 14803–6. http://dx.doi.org/10.1039/c4ra00214h.

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A facile and convenient microemulsion method is demonstrated to prepare fluorescent nanoscale coordination polymers. And the nanoscale coordination polymers exhibited bright blue fluorescence and good biocompatibility, thus giving them the ability for bioimaging.

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14

Li, Li, Jianbo Liu, Xiaohai Yang, Zhihong Peng, Wei Liu, Jianguo Xu, Jinlu Tang, Xiaoxiao He i Kemin Wang. "Quantum dot/methylene blue FRET mediated NIR fluorescent nanomicelles with large Stokes shift for bioimaging". Chemical Communications 51, nr 76 (2015): 14357–60. http://dx.doi.org/10.1039/c5cc06258f.

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We present a novel large Stokes shifting NIR fluorescent nanomicelle through the encapsulation of a quantum dot/methylene blue FRET pair, which is employed as an excellent contrast reagent for NIR fluorescence bioimaging.

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15

Zuo, Jun, Tao Jiang, Xiaojing Zhao, Xiaohong Xiong, Saijin Xiao i Zhiqiang Zhu. "Preparation and Application of Fluorescent Carbon Dots". Journal of Nanomaterials 2015 (2015): 1–13. http://dx.doi.org/10.1155/2015/787862.

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Fluorescent carbon dots (CDs) are a novel type of fluorescent nanomaterials, which not only possess the specific quantum confinement effects of nanomaterials due to the small size of nanomaterials, but also have good biocompatibility and high fluorescence. Meanwhile, fluorescence CDs overcome the shortcomings of high toxicity of traditional nanomaterials. Moreover, the preparation procedure of fluorescent CDs is simple and easy. Therefore, fluorescent CDs have great potential applied in photocatalysis, biochemical sensing, bioimaging, drug delivery, and other related areas. In this paper, recent hot researches on fluorescent CDs are reviewed and some problems in the progress of fluorescent CDs are also summarized. At last, a future outlook in this direction is presented.

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Esthar, Selvaraj, Raman Dhivya, U. Ramesh, Jegathalaprathaban Rajesh, Thomas J. Webster, Jamespandi Annaraj i Guruswamy Rajagopal. "Biocompatible, Biodegradable, and Improved Fluorescent Silicon Quantum Dots for Zebrafish Imaging". Journal of Biomedical Nanotechnology 18, nr 12 (1.12.2022): 2740–49. http://dx.doi.org/10.1166/jbn.2022.3436.

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One of the greatest benefits of nanomedicine elucidated to date includes the non-invasive tracking and monitoring of living organisms by the selective uptake of harmless metallic nanoparticles. Several nanoscale probes have been employed for biomolecular imaging. Among them, fluorescent nanoscale silicon materials have been recently established with a strong and safe potential for bioimaging and biosensing applications due to their bright fluorescence coupled with strong photostability, biocompatibility and negligible toxicity. Herein, we developed high-quality silicon nanomaterials (4–5 nm; SiNPs) as biological fluorescent probes for bioimaging of living organisms through an easy aquatic synthesis method with a quantum yield of ∼8%. In this regard, we report that the presently synthesized SiNPs-based sensors/probes are attractive materials for solvent-based fluorescence measurements and are biocompatible, non-toxic, highly photo-stable and pH stable. Most importantly, their fluorescence lifetime is much longer than that of native probes in living cells. Thus, these presently formulated SiNPs are improved fluorescent probes for in vivo biological imaging in zebra fish embryos as well as numerous other living organisms and, thus, should be further studied.

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An, Dongdong, Linlin Shi, Tianyu Li, Hong-Yu Zhang, Yahong Chen, Xin-Qi Hao i Mao-Ping Song. "Tailored Supramolecular Cage for Efficient Bio-Labeling". International Journal of Molecular Sciences 24, nr 3 (21.01.2023): 2147. http://dx.doi.org/10.3390/ijms24032147.

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Fluorescent chemosensors are powerful imaging tools used in a broad range of biomedical fields. However, the application of fluorescent dyes in bioimaging still remains challenging, with small Stokes shifts, interfering signals, background noise, and self-quenching on current microscope configurations. In this work, we reported a supramolecular cage (CA) by coordination-driven self-assembly of benzothiadiazole derivatives and Eu(OTf)3. The CA exhibited high fluorescence with a quantum yield (QY) of 38.57%, good photoluminescence (PL) stability, and a large Stokes shift (153 nm). Furthermore, the CCK-8 assay against U87 glioblastoma cells verified the low cytotoxicity of CA. We revealed that the designed probes could be used as U87 cells targeting bioimaging.

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Ahmmed, Ejaj, Somenath Lohar, Sumanta Ghatak, Sumit Kumar Hira, Partha Pratim Manna i Pabitra Chattopadhyay. "Development of a selective reaction-based turn-on fluorosensor and biomarker for hypochlorite ions in aqueous media". Analytical Methods 11, nr 18 (2019): 2415–21. http://dx.doi.org/10.1039/c9ay00325h.

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A new structurally characterised non-fluorescent non-cytotoxic probe (LH) selectively senses trace level hypochlorite ions in aqueous medium at pH 7.4 by turn-on ICT based fluorescence through thioacetyl cleavage applicable in bioimaging.

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Lu, Yanjiao, Bicheng He, Jie Shen, Jie Li, Wantai Yang i Meizhen Yin. "Multifunctional magnetic and fluorescent core–shell nanoparticles for bioimaging". Nanoscale 7, nr 5 (2015): 1606–9. http://dx.doi.org/10.1039/c4nr06104g.

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Magnetic and fluorescent core–shell nanoparticles exhibit superparamagnetic behavior and emit strong near-infrared fluorescence. The nanoparticles are highly biocompatible and accumulate in cell nuclei via strong interaction with nucleic acids.

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Wan, Jiafeng, Xiaoyuan Zhang, Kai Zhang i Zhiqiang Su. "Biological nanoscale fluorescent probes: From structure and performance to bioimaging". Reviews in Analytical Chemistry 39, nr 1 (1.01.2020): 209–21. http://dx.doi.org/10.1515/revac-2020-0119.

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Abstract In recent years, nanomaterials have attracted lots of attention from researchers due to their unique properties. Nanometer fluorescent materials, such as organic dyes, semiconductor quantum dots (QDs), metal nano-clusters (MNCs), carbon dots (CDs), etc., are widely used in biological imaging due to their high sensitivity, short response time, and excellent accuracy. Nanometer fluorescent probes can not only perform in vitro imaging of organisms but also achieve in vivo imaging. This provides medical staff with great convenience in cancer treatment. Combined with contemporary medical methods, faster and more effective treatment of cancer is achievable. This article explains the response mechanism of three-nanometer fluorescent probes: the principle of induced electron transfer (PET), the principle of fluorescence resonance energy transfer (FRET), and the principle of intramolecular charge transfer (ICT), showing the semiconductor QDs, precious MNCs, and CDs. The excellent performance of the three kinds of nano fluorescent materials in biological imaging is highlighted, and the application of these three kinds of nano fluorescent probes in targeted biological imaging is also introduced. Nanometer fluorescent materials will show their significance in the field of biomedicine.

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Cheruku, Srujan, Lien D’Olieslaeger, Nick Smisdom, Joeri Smits, Dirk Vanderzande, Wouter Maes, Marcel Ameloot i Anitha Ethirajan. "Fluorescent PCDTBT Nanoparticles with Tunable Size for Versatile Bioimaging". Materials 12, nr 15 (6.08.2019): 2497. http://dx.doi.org/10.3390/ma12152497.

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Conjugated polymer nanoparticles exhibit very interesting properties for use as bio-imaging agents. In this paper, we report the synthesis of PCDTBT (poly([9-(1’-octylnonyl)-9H-carbazole-2,7-diyl]-2,5-thiophenediyl-2,1,3-benzothiadiazole-4,7-diyl-2,5-thiophene-diyl)) nanoparticles of varying sizes using the mini-emulsion and emulsion/solvent evaporation approach. The effect of the size of the particles on the optical properties is investigated using UV-Vis absorption and fluorescence emission spectroscopy. It is shown that PCDTBT nanoparticles have a fluorescence emission maximum around 710 nm, within the biological near-infrared “optical window”. The photoluminescence quantum yield shows a characteristic trend as a function of size. The particles are not cytotoxic and are taken up successfully by human lung cancer carcinoma A549 cells. Irrespective of the size, all particles show excellent fluorescent brightness for bioimaging. The fidelity of the particles as fluorescent probes to study particle dynamics in situ is shown as a proof of concept by performing raster image correlation spectroscopy. Combined, these results show that PCDTBT is an excellent candidate to serve as a fluorescent probe for near-infrared bio-imaging.

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Xu, Xiao Qing. "Methylthiouracil-Templated Formation of the Fluorescent Silver Nanocluster and its Application to Bioimaging". Advanced Materials Research 937 (maj 2014): 256–59. http://dx.doi.org/10.4028/www.scientific.net/amr.937.256.

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Herein, we report a novel approach to Methylthiouracil-templated formation of fluorescent Ag nanoclusters and its application to bioimaging. We used fluorescence assay and TEM to characterize the silver nanocluster. The complex can be directly employed into bioimaging HeLa cells. The cell toxicity (3-[4,5-dimethylthiazolyl-2]-2,5-diphenyltetrazolium bromide, MTT) assay demonstrated that the silver nanocluster has only little affect on the cytotoxicity to the cells, which further proves the applicability of the method in tumor cell imaging. This work successfully develops the capping Methylthiouracil scaffolds of silver nanoclusters.

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Nifontova, Galina, Victor Krivenkov, Mariya Zvaigzne, Anton Efimov, Evgeny Korostylev, Sergei Zarubin, Alexander Karaulov, Igor Nabiev i Alyona Sukhanova. "Nanoparticle-Doped Hybrid Polyelectrolyte Microcapsules with Controlled Photoluminescence for Potential Bioimaging Applications". Polymers 13, nr 23 (24.11.2021): 4076. http://dx.doi.org/10.3390/polym13234076.

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Fluorescent imaging is widely used in the diagnosis and tracking of the distribution, interaction, and transformation processes at molecular, cellular, and tissue levels. To be detectable, delivery systems should exhibit a strong and bright fluorescence. Quantum dots (QDs) are highly photostable fluorescent semiconductor nanocrystals with wide absorption spectra and narrow, size-tunable emission spectra, which make them suitable fluorescent nanolabels to be embedded into microparticles used as bioimaging and theranostic agents. The layer-by-layer deposition approach allows the entrapping of QDs, resulting in bright fluorescent microcapsules with tunable surface charge, size, rigidity, and functional properties. Here, we report on the engineering and validation of the structural and photoluminescent characteristics of nanoparticle-doped hybrid microcapsules assembled by the deposition of alternating oppositely charged polyelectrolytes, water-soluble PEGylated core/shell QDs with a cadmium selenide core and a zinc sulfide shell (CdSe/ZnS), and carboxylated magnetic nanoparticles (MNPs) onto calcium carbonate microtemplates. The results demonstrate the efficiency of the layer-by-layer approach to designing QD-, MNP-doped microcapsules with controlled photoluminescence properties, and pave the way for the further development of next-generation bioimaging agents based on hybrid materials for continuous fluorescence imaging.

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Phan, Le Minh Tu, i Sungbo Cho. "Fluorescent Carbon Dot-Supported Imaging-Based Biomedicine: A Comprehensive Review". Bioinorganic Chemistry and Applications 2022 (10.04.2022): 1–32. http://dx.doi.org/10.1155/2022/9303703.

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Carbon dots (CDs) provide distinctive advantages of strong fluorescence, good photostability, high water solubility, and outstanding biocompatibility, and thus are widely exploited as potential imaging agents for in vitro and in vivo bioimaging. Imaging is absolutely necessary when discovering the structure and function of cells, detecting biomarkers in diagnosis, tracking the progress of ongoing disease, treating various tumors, and monitoring therapeutic efficacy, making it an important approach in modern biomedicine. Numerous investigations of CDs have been intensively studied for utilization in bioimaging-supported medical sciences. However, there is still no article highlighting the potential importance of CD-based bioimaging to support various biomedical applications. Herein, we summarize the development of CDs as fluorescence (FL) nanoprobes with different FL colors for potential bioimaging-based applications in living cells, tissue, and organisms, including the bioimaging of various cell types and targets, bioimaging-supported sensing of metal ions and biomolecules, and FL imaging-guided tumor therapy. Current CD-based microscopic techniques and their advantages are also highlighted. This review discusses the significance of advanced CD-supported imaging-based in vitro and in vivo investigations, suggests the potential of CD-based imaging for biomedicine, and encourages the effective selection and development of superior probes and platforms for further biomedical applications.

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Gonçalves, Raquel C. R., Efres Belmonte-Reche, João Pina, Milene Costa da Silva, Sónia C. S. Pinto, Juan Gallo, Susana P. G. Costa i M. Manuela M. Raposo. "Bioimaging of Lysosomes with a BODIPY pH-Dependent Fluorescent Probe". Molecules 27, nr 22 (20.11.2022): 8065. http://dx.doi.org/10.3390/molecules27228065.

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Fluorescence-based probes represent a powerful tool for noninvasive imaging of living systems in real time and with a high temporal and spatial resolution. Amongst several known fluorophores, 3-difluoroborodipyrromethene (BODIPY) derivatives have become a cornerstone for innovative fluorescent labelling applications, mainly due to their advantageous features including their facile synthesis, structural versatility and exceptional photophysical properties. In this context, we report a BODIPY-based fluorescent probe for imaging of lysosomes in living cells. The BODIPY derivative displayed a remarkable fluorescence enhancement at low pH values with a pKa* of 3.1. In vitro studies by confocal microscopy in HeLa cells demonstrated that the compound was able to permeate cell membrane and selectively label lysosome whilst remaining innocuous to the cell culture at the maximum concentration tested. Herein, the BODIPY derivative holds the promise of investigating lysosomal dynamics and function in living cells through fluorescence imaging.

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Qu, Xiaochao, Yichen Li, Lei Li, Yanran Wang, Jingning Liang i Jimin Liang. "Fluorescent Gold Nanoclusters: Synthesis and Recent Biological Application". Journal of Nanomaterials 2015 (2015): 1–23. http://dx.doi.org/10.1155/2015/784097.

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Fluorescent gold nanoclusters (AuNCs) have been extensively studied due to their unique construction and distinctive properties, which place them between single metal atoms and larger nanoparticles. The dimension of AuNCs is comparable to the Fermi wavelength of electrons, which lead to size-dependent fluorescence and other molecule-like properties. In this review, we summarize various synthesis strategies of fluorescent AuNCs and recent advances of biological applications such as biosensing, biolabeling, and bioimaging. The synthetic methods are considered as two routes: “Atoms to Clusters” and “Nanoparticles to Clusters.” The surface functionalization of AuNCs is described as the precondition for making future bioapplications possible, which can eventually influence their stability, biocompatibility, and other properties. And then we focus on the recent advances of AuNCs-based applications in biological sensing, biolabeling, and bioimaging and finally discuss the current challenges of AuNCs in controllable synthesis and biological application.

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Song, Yubin, Shoujun Zhu i Bai Yang. "Bioimaging based on fluorescent carbon dots". RSC Advances 4, nr 52 (2014): 27184. http://dx.doi.org/10.1039/c3ra47994c.

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Yao, Sheng, i Kevin D. Belfield. "Two-Photon Fluorescent Probes for Bioimaging". European Journal of Organic Chemistry 2012, nr 17 (7.05.2012): 3199–217. http://dx.doi.org/10.1002/ejoc.201200281.

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Jańczewski, Dominik, Yan Zhang, Gautom Kumar Das, Dong Kee Yi, Parasuraman Padmanabhan, Kishore K. Bhakoo, Timothy Thatt Yang Tan i Subramanian Tamil Selvan. "Bimodal magnetic-fluorescent probes for bioimaging". Microscopy Research and Technique 74, nr 7 (23.08.2010): 563–76. http://dx.doi.org/10.1002/jemt.20912.

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Torelli, Marco D., Nicholas A. Nunn i Olga A. Shenderova. "A Perspective on Fluorescent Nanodiamond Bioimaging". Small 15, nr 48 (19.06.2019): 1902151. http://dx.doi.org/10.1002/smll.201902151.

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Pu, Kan-Yi, i Bin Liu. "Bioimaging: Fluorescent Conjugated Polyelectrolytes for Bioimaging (Adv. Funct. Mater. 18/2011)". Advanced Functional Materials 21, nr 18 (20.09.2011): 3407. http://dx.doi.org/10.1002/adfm.201190076.

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Li, Weitao, Ningjia Jiang, Bin Wu, Yuan Liu, Luoman Zhang i Jianxin He. "Chlorine Modulation Fluorescent Performance of Seaweed-Derived Graphene Quantum Dots for Long-Wavelength Excitation Cell-Imaging Application". Molecules 26, nr 16 (18.08.2021): 4994. http://dx.doi.org/10.3390/molecules26164994.

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Biological imaging is an essential means of disease diagnosis. However, semiconductor quantum dots that are used in bioimaging applications comprise toxic metal elements that are nonbiodegradable, causing serious environmental problems. Herein, we developed a novel ecofriendly solvothermal method that uses ethanol as a solvent and doping with chlorine atoms to prepare highly fluorescent graphene quantum dots (GQDs) from seaweed. The GQDs doped with chlorine atoms exhibit high-intensity white fluorescence. Thus, their preliminary application in bioimaging has been confirmed. In addition, clear cell imaging could be performed at an excitation wavelength of 633 nm.

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Bai, Yunlong, Tong Shu, Lei Su i Xueji Zhang. "Fluorescent Gold Nanoclusters for Biosensor and Bioimaging Application". Crystals 10, nr 5 (30.04.2020): 357. http://dx.doi.org/10.3390/cryst10050357.

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With the rapid development of materials technology, fluorescent gold nanoclusters (AuNCs) are emerging as novel functional materials for diagnostic applications including the detection of biomarkers and bioimaging due to the advantages of their ultra-small size, tunable emissions, size-dependent fluorescence and excellent biocompatibility. In this review, we introduced the synthetic methods, and physical and chemical properties of AuNCs. Subsequently, we described the AuNCs-based design strategies for the detection of biomarkers including small molecules, DNA and proteins. The applications of AuNCs for tumor imaging in vitro and in vivo were also presented. Finally, we discussed the challenges and potential solutions of AuNCs-based nanosensors.

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Das, Gobinda, Farah Benyettou, Sudhir Kumar Sharama, Thirumurugan Prakasam, Felipe Gándara, Victor A. de la Peña-O’Shea, Na'il Saleh i in. "Covalent organic nanosheets for bioimaging". Chemical Science 9, nr 44 (2018): 8382–87. http://dx.doi.org/10.1039/c8sc02842g.

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Li, Dong, Jie Pan, Shuyu Xu, Shiying Fu, Chengchao Chu i Gang Liu. "Activatable Second Near-Infrared Fluorescent Probes: A New Accurate Diagnosis Strategy for Diseases". Biosensors 11, nr 11 (2.11.2021): 436. http://dx.doi.org/10.3390/bios11110436.

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Recently, second near-infrared (NIR-II) fluorescent imaging has been widely applied in biomedical diagnosis, due to its high spatiotemporal resolution and deep tissue penetration. In contrast to the “always on” NIR-II fluorescent probes, the activatable NIR-II fluorescent probes have specific targeting to biological tissues, showing a higher imaging signal-to-background ratio and a lower detection limit. Therefore, it is of great significance to utilize disease-associated endogenous stimuli (such as pH values, enzyme existence, hypoxia condition and so on) to activate the NIR-II probes and achieve switchable fluorescent signals for specific deep bioimaging. This review introduces recent strategies and mechanisms for activatable NIR-II fluorescent probes and their applications in biosensing and bioimaging. Moreover, the potential challenges and perspectives of activatable NIR-II fluorescent probes are also discussed.

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Chen, Zixu, Shengyu Feng i Dengxu Wang. "Organosilicon Fluorescent Materials". Polymers 15, nr 2 (9.01.2023): 332. http://dx.doi.org/10.3390/polym15020332.

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In the past few decades, organosilicon fluorescent materials have attracted great attention in the field of fluorescent materials not only due to their abundant and flexible structures, but also because of their intriguing fluorescence properties, distinct from silicon-free fluorescent materials. Considering their unique properties, they have found broad application prospects in the fields of chemosensor, bioimaging, light-emitting diodes, etc. However, a comprehensive review focusing on this field, from the perspective of their catalogs and applications, is still absent. In this review, organosilicon fluorescent materials are classified into two main types, organosilicon small molecules and polymers. The former includes fluorescent aryl silanes and siloxanes, and the latter are mainly fluorescent polysiloxanes. Their synthesis and applications are summarized. In particular, the function of silicon atoms in fluorescent materials is introduced. Finally, the development trend of organosilicon fluorescent materials is prospected.

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Ganguly, Sayan, i Shlomo Margel. "Bioimaging Probes Based on Magneto-Fluorescent Nanoparticles". Pharmaceutics 15, nr 2 (17.02.2023): 686. http://dx.doi.org/10.3390/pharmaceutics15020686.

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Novel nanomaterials are of interest in biology, medicine, and imaging applications. Multimodal fluorescent-magnetic nanoparticles demand special attention because they have the potential to be employed as diagnostic and medication-delivery tools, which, in turn, might make it easier to diagnose and treat cancer, as well as a wide variety of other disorders. The most recent advancements in the development of magneto-fluorescent nanocomposites and their applications in the biomedical field are the primary focus of this review. We describe the most current developments in synthetic methodologies and methods for the fabrication of magneto-fluorescent nanocomposites. The primary applications of multimodal magneto-fluorescent nanoparticles in biomedicine, including biological imaging, cancer treatment, and drug administration, are covered in this article, and an overview of the future possibilities for these technologies is provided.

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Kapil, Kriti, Shirley Xu, Inseon Lee, Hironobu Murata, Seok-Joon Kwon, Jonathan S. Dordick i Krzysztof Matyjaszewski. "Highly Sensitive Detection of Bacteria by Binder-Coupled Multifunctional Polymeric Dyes". Polymers 15, nr 12 (18.06.2023): 2723. http://dx.doi.org/10.3390/polym15122723.

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Infectious diseases caused by pathogens are a health burden, but traditional pathogen identification methods are complex and time-consuming. In this work, we have developed well-defined, multifunctional copolymers with rhodamine B dye synthesized by atom transfer radical polymerization (ATRP) using fully oxygen-tolerant photoredox/copper dual catalysis. ATRP enabled the efficient synthesis of copolymers with multiple fluorescent dyes from a biotin-functionalized initiator. Biotinylated dye copolymers were conjugated to antibody (Ab) or cell-wall binding domain (CBD), resulting in a highly fluorescent polymeric dye-binder complex. We showed that the unique combination of multifunctional polymeric dyes and strain-specific Ab or CBD exhibited both enhanced fluorescence and target selectivity for bioimaging of Staphylococcus aureus by flow cytometry and confocal microscopy. The ATRP-derived polymeric dyes have the potential as biosensors for the detection of target DNA, protein, or bacteria, as well as bioimaging.

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De Leo, Vincenzo, Francesco Milano, Aurora Paiano, Roberta Bramato, Livia Giotta, Roberto Comparelli, Silvia Ruscigno, Angela Agostiano, Cecilia Bucci i Lucia Catucci. "Luminescent CdSe@ZnS nanocrystals embedded in liposomes: a cytotoxicity study in HeLa cells". Toxicol. Res. 6, nr 6 (2017): 947–57. http://dx.doi.org/10.1039/c7tx00172j.

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Bertelà, Federica, Chiara Battocchio, Giovanna Iucci, Simona Ceschin, Dario Di Lernia, Flaminia Mariani, Andrea Di Giulio, Maurizio Muzzi i Iole Venditti. "Dye-Doped Polymeric Microplastics: Light Tools for Bioimaging in Test Organisms". Polymers 15, nr 15 (30.07.2023): 3245. http://dx.doi.org/10.3390/polym15153245.

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Ecosystems around the world are experiencing a major environmental impact from microplastic particles (MPs 0.1 µm–1 mm). Water, sediments, and aquatic biota show the widespread presence of this pollutant. However, MPs are rarely used in laboratory studies as they are scarcely available for purchase or expensive, especially if one wishes to trace the particle with a dye or fluorescent. Furthermore, existing preparation techniques have limited application in biological studies. In this work, we propose a new, easy, and cheap way to prepare fluorescent MPs. The protocol is based on the osmosis method in order to obtain spherical polymeric particles of P(S-co-MMA), with 0.7–9 micron diameter, made fluorescent because dye-doped with rhodamine B isothiocyanate (RITC) or fluorescein isothiocyanate (FITC). The dye loading was studied and optimized, and the MPs–dye conjugates were characterized by UV-vis FTIR and XPS spectrometry and scanning electron microscopy (SEM). Furthermore, preliminary tests on aquatic organisms demonstrated the possible use of these fluorescent MPs in bioimaging studies, showing their absorption/adsorption by duckweeds (Lemna minuta) and insect larvae (Cataclysta lemnata).

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41

Selvaraj, Joicy, Arun Mahesh, Vaseeharan Baskaralingam, Arunkumar Dhayalan i Thangadurai Paramasivam. "Organic-to-water dispersible Mn:ZnS–ZnS doped core–shell quantum dots: synthesis, characterization and their application towards optical bioimaging and a turn-off fluorosensor". New Journal of Chemistry 43, nr 30 (2019): 11912–25. http://dx.doi.org/10.1039/c9nj02222h.

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Fan, Zetan, Shuhua Li, Fanglong Yuan i Louzhen Fan. "Fluorescent graphene quantum dots for biosensing and bioimaging". RSC Advances 5, nr 25 (2015): 19773–89. http://dx.doi.org/10.1039/c4ra17131d.

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Prandi, Cristina, Giovanni Ghigo, Ernesto G. Occhiato, Dina Scarpi, Stefano Begliomini, Beatrice Lace, Gabriele Alberto, Emma Artuso i Marco Blangetti. "Tailoring fluorescent strigolactones for in vivo investigations: a computational and experimental study". Org. Biomol. Chem. 12, nr 18 (2014): 2960–68. http://dx.doi.org/10.1039/c3ob42592d.

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NAGANO, Tetsuo. "Development of fluorescent probes for bioimaging applications". Proceedings of the Japan Academy, Series B 86, nr 8 (2010): 837–47. http://dx.doi.org/10.2183/pjab.86.837.

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Dias, Gleiston G., Pamella V. B. Pinho, Hélio A. Duarte, Jarbas M. Resende, Andressa B. B. Rosa, José R. Correa, Brenno A. D. Neto i Eufrânio N. da Silva Júnior. "Fluorescent oxazoles from quinones for bioimaging applications". RSC Advances 6, nr 79 (2016): 76056–63. http://dx.doi.org/10.1039/c6ra14701a.

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This work describes a synthetic strategy for the syntheses of four new fluorescent excited state intramolecular proton transfer (ESIPT) prone oxazole derivatives synthesized from lapachol, a naturally occurring naphthoquinone isolated from the Tabebuia species (ipe tree).

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Koktysh, Dmitry, Vanessa Bright i Wellington Pham. "Fluorescent magnetic hybrid nanoprobe for multimodal bioimaging". Nanotechnology 22, nr 27 (20.05.2011): 275606. http://dx.doi.org/10.1088/0957-4484/22/27/275606.

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Girija Aswathy, Ravindran, Balasubramanian Sivakumar, Dhandayudhapani Brahatheeshwaran, Tomofumi Ukai, Yasuhiko Yoshida, Toru Maekawa i Sakthi D. Kumar. "Biocompatible Fluorescent Jelly Quantum Dots for Bioimaging". Materials Express 1, nr 4 (1.12.2011): 291–98. http://dx.doi.org/10.1166/mex.2011.1045.

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Li, Zhen, Qiao Sun, Yian Zhu, Bien Tan, Zhi Ping Xu i Shi Xue Dou. "Ultra-small fluorescent inorganic nanoparticles for bioimaging". J. Mater. Chem. B 2, nr 19 (2014): 2793–818. http://dx.doi.org/10.1039/c3tb21760d.

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The recent advances of ultra-small fluorescence inorganic nanoparticles including quantum dots, metal nanoclusters, carbon and graphene dots, up-conversion nanocrystals, and silicon nanoparticles have been comprehensively reviewed.

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Merchant, Kelsie, i Susanta K. Sarkar. "Fluorescent Nanodiamonds for Molecular and Cellular Bioimaging". IEEE Journal of Selected Topics in Quantum Electronics 22, nr 3 (maj 2016): 235–45. http://dx.doi.org/10.1109/jstqe.2015.2512981.

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Shedden, Kerby, i Gus R. Rosania. "Chemical address tags of fluorescent bioimaging probes". Cytometry Part A 9999A (2010): NA. http://dx.doi.org/10.1002/cyto.a.20847.

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