Gotowe bibliografie tematyczne / Faecol sterols

Gotowa bibliografia na temat „Faecol sterols”

Autor: Grafiati
Data publikacji: 4 czerwca 2021
Data aktualizacji: 20 lutego 2023

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Artykuły w czasopismach na temat "Faecol sterols"

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Nakamura, T., H. Kikuchi, K. Takebe, K. Kudoh, A. Terada, Y. Tan-Doh i N. Yamada. "Faecal Lipid Excretion Levels in Normal Japanese Females on an Unrestricted Diet and a Fat-Restricted Diet Measured by Simultaneous Analysis of Faecal Lipids". Journal of International Medical Research 20, nr 6 (listopad 1992): 461–66. http://dx.doi.org/10.1177/030006059202000603.

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Faecal lipid excretion was determined in 16 females on an unrestricted diet and on a fat-restricted diet using a chromatographic method for the simultaneous analysis of faecal lipids. The fat-restricted diet reduced the total quantity of faeces and the amounts of fatty acids, neutral sterols and bile acids excreted were almost halved compared with when on an unrestricted diet. This indicates that dietary fat, fibre and cholesterol affect the amount of faecal bile acid, neutral sterol and fatty acid excretion. The amount of cholesterol/animal sterols excreted and the percentage of primary bile acids were, however, similar for both the fat-restricted and unrestricted diets.
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Lerch, Marcel, Tobias Bromm, Clemens Geitner, Jean Nicolas Haas, Dieter Schäfer, Bruno Glaser i Michael Zech. "Human and livestock faecal biomarkers at the prehistorical encampment site of Ullafelsen in the Fotsch Valley, Stubai Alps, Austria – potential and limitations". Biogeosciences 19, nr 4 (23.02.2022): 1135–50. http://dx.doi.org/10.5194/bg-19-1135-2022.

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Abstract. The Ullafelsen at 1869 m above sea level (a.s.l.) in the Tyrolean Stubai Alps next to Innsbruck is an important (geo)archeological reference site for the Mesolithic period. Buried fireplaces on the Ullafelsen plateau were dated at 10.9 to 9.5 ka cal BP and demonstrate together with thousands of flint stone artifacts the presence of hunter-gatherers during the Early Holocene. Grazing livestock has been a predominant anthropozoological impact in the Fotsch Valley presumably since the Bronze Age (4.2–2.8 ka). In order to study the human and/or livestock faeces input on the Ullafelsen, we carried out steroid analyses on 2 modern ruminant faeces samples from cattle and sheep, 37 soil samples from seven archeological soil profiles, and 9 soil samples from five non-archeological soil profiles from the Fotsch Valley used as reference sites. The dominance of 5β-stigmastanol and deoxycholic acid in modern cattle and sheep faeces can be used as markers for the input of ruminant faeces in soils. The OAh horizons, which have accumulated and developed since the Mesolithic, revealed high contents of steroids (sterols, stanols, stanones and bile acids); the eluvial light layer (E (LL)) horizon coinciding with the Mesolithic living floor is characterized by medium contents of steroids. By contrast, the subsoil horizons Bh, Bs and BvCv contain low contents of faecal biomarkers, indicating that leaching of steroids into the podsolic subsoils is not an important factor. High content of 5β-stigmastanol and deoxycholic acid in all soil samples gives evidence for faeces input of ruminants. The steroid patterns and ratios indicate a negligible input of human faeces on the Ullafelsen. In conclusion, our results reflect a strong faeces input by livestock, rather than by humans as found for other Anthrosols such as Amazonian dark earths. Further studies need to focus on the question of the exact timing of faeces deposition.
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Suprihatin, I., H. Fallowfield, R. Bentham i N. Cromar. "Determination of faecal pollutants in Torrens and Patawalonga catchment waters in South Australia using faecal sterols". Water Science and Technology 47, nr 7-8 (1.04.2003): 283–89. http://dx.doi.org/10.2166/wst.2003.0700.

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This research investigated faecal pollution by sterol profiling in two Adelaide metropolitan catchments in winter 2000 and summer 2001. Water was sampled from five sites on the River Torrens and two sites on Patawalonga river catchment. The steroids detected were coprostanol, epicoprostanol, cholesterol, cholestanol, stigmasterol, betasitosterol, and stigmastanol. The concentrations range from 5 ng/L (of coprostanol) to 34 μmg/L (of stigmasterol), with cholesterol detected more frequently at relatively high concentrations (approximately 2 μmg/L). Sterol profiling suggested two sites on two occasions may have been impacted by human faecal contamination. Other steroid profiles suggested that sources of pollution consistently included dogs and birds. The steroid concentrations in the Torrens catchment were higher in winter, however, this was not observed in the Patawalonga catchment.
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Reddy, Sheela, T. A. B. Sanders, R. W. Owen i M. H. Thompson. "Faecal pH, bile acid and sterol concentrations in premenopausal Indian and white vegetarians compared with white omnivores". British Journal of Nutrition 79, nr 6 (czerwiec 1998): 495–500. http://dx.doi.org/10.1079/bjn19980087.

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Faecal bulk, pH, water content, the concentrations of neutral sterols and bile acids and dietary intakes were measured in twenty-two Indian vegetarian, twenty-two white omnivorous and eighteen white vegetarian premenopausal women. Faecal bulk and water content were greater and pH lower in the Indian vegetarians. Total faecal animal sterol and coprostanol concentrations expressed on a dry-weight basis were lower in the vegetarians compared with the omnivores. The faecal sterol concentrations were correlated with dietary cholesterol intake. Primary bile acids were detected in six Indian vegetarians, two white vegetarians and two white omnivores; secondary bile acids were detected in all the white omnivore and vegetarian subjects but not in two of the Indian vegetarians. Total faecal free bile acid and conjugated bile acid concentrations were lower in the white vegetarians compared with the omnivores. Faecal lithocholic acid concentrations were lower in both Indian and white vegetarians. The lithocholic: deoxycholic acid ratio and coprostanol: total animal sterols ratio were significantly lower in the Indian vegetarians compared with the omnivores. Both ratios were positively correlated with faecal pH. Stepwise multiple regression analyses were undertaken in order to identify which nutrients influenced faecal pH, lithocholic and deoxycholic acid concentrations. The intakes of starch and dietary fibre were negatively associated with faecal concentrations of lithocholic and deoxycholic acid. Starch intake alone was negatively associated with faecal pH. The results of this study confirm that diets high in dietary fibre decrease faecal bile acid concentrations and suggest that the complex carbohydrates present in Indian vegetarian diets influence faecal pH and inhibit the degradation of faecal steroids.
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Huanling, Yu, Li Yong, Wang Junbo, Zheng Liping i Yan Weixing. "Chinese soft-shelled turtle egg powder lowers serum cholesterol, increases faecal neutral steroids and bile acid excretion, and up-regulates liver cytochrome P450 mRNA level in rats". British Journal of Nutrition 94, nr 3 (wrzesień 2005): 315–20. http://dx.doi.org/10.1079/bjn20051485.

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The aim of the present study was to investigate the effect of Chinese soft-shelled turtle whole egg powder (TE) on cholesterol metabolism in Sprague–Dawley rats to determine whether it has a cholesterol-lowering effect. Forty male Sprague–Dawley rats were fed a high-fat diet supplemented with TE (0, 0·75, 1·50 or 3·00 g/kg body weight) administrated by gavage for 24 weeks. Serum total cholesterol (TC), HDL-cholesterol (HDL-C), LDL-cholesterol (LDL-C) and faecal total bile acids levels were determined by enzymatic methods. Faecal steroid concentrations were measured by GC. Means and standard deviations were calculated where appropriate for values, and the data were tested by one-way ANOVA. After 24 weeks of feeding a high-fat diet with TE supplementation, serum TC and LDL-C levels, liver cholesterol and liver lipid levels were reduced in rats. TE supplementation did not affect the faecal output, but significantly increased steroid concentrations in faeces, indicating increased steroids excretion. The faecal bile acid excretion was also increased as evidence by elevated mRNA level of liver cytochrome P450, family 7, subfamily A, polypeptide 1. Our results demonstrated that the TE does have a cholesterol-lowering effect by increasing the excretion of total bile acids and neutral steroids.
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Sullivan, D., P. Brooks, N. Tindale, S. Chapman i W. Ahmed. "Faecal sterols analysis for the identification of human faecal pollution in a non-sewered catchment". Water Science and Technology 61, nr 5 (1.03.2010): 1355–61. http://dx.doi.org/10.2166/wst.2010.227.

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In this study, faecal sterols were used to identify human faecal pollution in a non-sewered catchment in Southeast Queensland, Australia. In all, 36 water samples were collected from six sites on six occasions and the concentration of sterols were determined using gas chromatography and mass spectrometry. The stanols concentration in water samples generally increased with increased catchment runoff. After moderate rainfall, high coprostanols levels found in water samples indicated human faecal pollution via defective septic systems. In contrast, it appears that during dry weather human faecal pollution is not occurring in the study catchment. Sterol profiles also pointed to a cattle farm polluting during modest catchment runoff. The method used in this study was able to identify the sources of faecal pollution to the catchment due to rainfall.
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Leeming, R., N. Bate, R. Hewlett i P. D. Nichols. "Discriminating faecal pollution: a case study of stormwater entering Port Phillip Bay, Australia". Water Science and Technology 38, nr 10 (1.11.1998): 15–22. http://dx.doi.org/10.2166/wst.1998.0369.

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This investigation was designed to provide preliminary information to the Environment Protection Authority concerning the input of faecal matter to stormwater drains in the Rippleside area of Geelong, Victoria. Results derived from the combined use of sterol biomarkers (e.g. coprostanol and 24-ethylcoprostanol) and four sub-groups of bacterial indicators (e.g. thermotolerant coliforms, E. coli, faecal streptococci and enterococci) indicated that during wet weather, all sites sampled were affected by significant human faecal contamination. Ratios of coprostanol to bacterial indicators were similar to those for samples collected from nearby sewer mains. During dry weather, there were still severely elevated levels of faecal contamination based on bacterial indicators, but correspondingly low concentrations of faecal sterols suggesting minimal human or herbivore faecal contamination. The origin of the majority of the faecal pollution in dry weather therefore remains to be fully explained. It is clear from this and related studies that the combined measurement of faecal sterols and bacterial indicators can greatly assist distinguishing sources of faecal pollution. It is also shown for aquatic environments that the measurement of coprostanol or other single indicators alone, is inadequate to fully discern faecal contamination from human sources.
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Green, Graham, i Peter D. Nichols. "Hydrocarbons and sterols in marine sediments and soils at Davis Station, Antarctica: a survey for human-derived contaminants". Antarctic Science 7, nr 2 (czerwiec 1995): 137–44. http://dx.doi.org/10.1017/s0954102095000198.

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A survey of hydrocarbons and sterols in marine and shoreline sediments was undertaken adjacent to Davis Station in Princess Elizabeth Land, Prydz Bay, Eastern Antarctica to determine the impact of a human settlement, including a sewage outfall on the local marine environment. Soil samples from selected locations onshore were also analysed to ascertain the extent of hydrocarbon contamination emanating from fuel storage facilities and other potential sources. The faecal sterol coprostanol was detected at 13.2 μg g−1 (60% of total sterols) in sediment adjacent to the Davis sewage outfall and up to 5.0 μg g−1 on the shoreline at Davis Beach. These concentrations indicate significant faecal contamination. The absence of coprostanol in faeces from the local wildlife confirms a human origin for this sewage biomarker. Hydrocarbons on the shoreline near Davis were present at up to 5.5 μg g−1 (dry weight of sediment). Biomarker profiles indicate an anthropogenic origin for these hydrocarbons. Onshore, degraded hydrocarbons derived from Special Antarctic Blend distillate were found at relatively high levels in soils at the fuel storage depot (up to 220 μg g−1). The source of these hydrocarbons appeared to be spillage from fuel storage tanks with possible contributions from fuel pipeline leakage and vehicle useage. Concentrations of polycyclic aromatic hydrocarbons in the soils were very low, generally below 1 ng g−1 (dry weight of sediment) for individual compounds.
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Leeming, Rhys, Jonathan S. Stark i James J. Smith. "Novel use of faecal sterols to assess human faecal contamination in Antarctica: a likelihood assessment matrix for environmental monitoring". Antarctic Science 27, nr 1 (25.06.2014): 31–43. http://dx.doi.org/10.1017/s0954102014000273.

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AbstractWastewater containing human sewage is often discharged with little or no treatment into the Antarctic marine environment. Faecal sterols (primarily coprostanol) in sediments have been used for assessment of human sewage contamination in this environment, butin situproduction and indigenous faunal inputs can confound such determinations. Using gas chromatography with mass spectral detection profiles of both C27and C29sterols, potential sources of faecal sterols were examined in nearshore marine sediments, encompassing sites proximal and distal to the wastewater outfall at Davis Station. Faeces from indigenous seals and penguins were also examined. Faeces from several indigenous species contained significant quantities of coprostanol but not 24-ethylcoprostanol, which is present in human faeces.In situcoprostanol and 24-ethylcoprostanol production was identified by co-production of their respectiveepi-isomers at sites remote from the wastewater source and in high total organic matter sediments. A C29sterols-based polyphasic likelihood assessment matrix for human sewage contamination is presented, which distinguishes human from local fauna faecal inputs andin situproduction in the Antarctic environment. Sewage contamination was detected up to 1.5 km from Davis Station.
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Volkman, John K., Teresa O'Leary, Rhys Leeming, Peter D. Nichols i John K. Volkman. "Assessment of the sources, transport and fate of sewage-derived organic matter in Port Phillip Bay, Australia, using the signature lipid coprostanol". Marine and Freshwater Research 50, nr 6 (1999): 547. http://dx.doi.org/10.1071/mf98051.

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To evaluate the distribution of faecal material in Port Phillip Bay, surface sediments from sites throughout the bay and inflowing water courses were analysed for fatty acids and sterols. Water samples were also collected to identify possible sources of faecal contamination. Bay sediments had total fatty acid concentrations between 4 and 183 µg g–1 (dry weight) and total sterol concentrations between 0.6 and 39.2 µg g–1. Creek sediments contained more sterols, ranging from 22.8–148 µg g–1. These lipid distributions suggest that the bulk of the labile organic matter derives from marine microalgae, primarily diatoms. Coprostanol (5β-cholestan-3β-ol), a sterol often used as an indicator of faecal contamination, was also present. Concentrations ranged from <0.01–0.55 µg g–1 in surface sediments, with values over 0.25 µg g–1 and 5β-/5α-C27 stanol ratios greater than 0.4, indicative of sewage inputs. A clearer picture of distributions of sewage-derived organic matter was obtained when coprostanol was normalized to total organic matter rather than sediment dry weight. Areas showing higher coprostanol concentrations included those adjacent to the main sewage treatment plant and several low-volume drains and creeks, indicating localized problems of sewage contamination.
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Rozprawy doktorskie na temat "Faecol sterols"

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Pratt, Catherine, i n/a. "Investigations into Faecal Sterols and E.Coli as Indicators of Sewage and Non-Sewage Inputs into a Subtropical Estuarine Embayment System in South Eastern QLD, Australia". Griffith University. School of Environmental and Applied Science, 2006. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20070124.111827.

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Sewage pollution from humans, animal and domestic sources (land and agricultural run-off) are recognized as a major cause of deteriorating water quality along Australia's coastline. Management of water quality has primarily relied on the use of bacterial indicator methods. However the validity and source-specificity of these methods have been met with increasing reservations for several years now. A relatively recent methodology uses a different chemical biomarker approach using 'sterols', a group of compounds related to the common bio-membrane lipid cholesterol and its derivatives. Sterols can offer an additional diagnostic tool to distinguish and discriminate between sources of faecal contamination in marine, freshwater and estuarine environments in both sediments and the water column. This study investigates for the first time, the degradation of coprostanol and selected faecal sterols in 'natural' sediments from a highly mixed (marine and estuarine) sub-tropical environment following a simulated pollution event (primary effluent); the use of faecal sterols as an additional indicator for determining non-point source sewage discharges at popular anchorages in the Moreton Bay and Gold Coast Broadwater system; and the use of sterol ratios in the determination of the fate and transportation of nutrients from a Sewage Treatment Plant (STP) point-source outlet pipe during plant malfunction. The microcosm degradation experiment revealed that faecal and selected sterols are continually synthesised and degraded over time by auto- and hetero trophic organisms within the sediment matrix. Coprostanol was the only sterol to degrade continually, with only minor fluctuations over a time period of two months. Results from this degradation experiment further revealed a sharp decline of coprostanol within the first week. From this it could be concluded that, without any further addition, external inputs of coprostanol are reduced to background levels within this time period. Therefore, removal of coprostanol after six days was 94% and 73% in mud and sand, respectively. The removal of coprostanol was much higher in mud than sand, reflecting a higher level of microbial activity in muddy sediments for assimilation of sterols. The field study undertaken at popular anchorages in Moreton Bay and the Gold Coast Broadwater revealed extremely low levels of sterols and bacterial indicators over both a spatial and temporal scale consistent with a shallow, oligotrophic, highly dynamic sand dominated system. Even though sterols analysed were found at extremely low levels (mostly in the nano-gram range), they were found to be highly correlated and were successful in identifying an unexpected once off pollution event from a point source at Moreton Bay Island. Other than this one incident, both sterol and bacterial levels were consistently low even when anchorages were at full capacity. Thus, sewage from recreational vessels was found to have very little, if any, effect on the water quality at anchorages in Moreton Bay and Gold Coast Broadwater. The point-source study conducted during a local sewage treatment plant malfunction revealed that even though absolute concentrations of sterols did not change during this event, the distribution of sterols within the samples changed, hence changing the sterol ratios. Further, nutrients (mainly nitrogen) can be transported several kilometres by currents, flocculate out of the water column and settle out into the sediment in areas with low tidal and hydrological flushing. There, the nutrients can cause in situ production of sterols in sediments changing sterol ratios. Overall, this study revealed that analyses of sterol biomarkers have the potential to indicate nutrient inputs (such as nitrogen) as well as sewage, post-hoc pollution events at extremely low levels/high dilutions in coastal sediments.
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Pratt, Catherine. "Investigations into Faecal Sterols and E.Coli as Indicators of Sewage and Non-Sewage Inputs into a Subtropical Estuarine Embayment System in South Eastern QLD, Australia". Thesis, Griffith University, 2006. http://hdl.handle.net/10072/366621.

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Sewage pollution from humans, animal and domestic sources (land and agricultural run-off) are recognized as a major cause of deteriorating water quality along Australia's coastline. Management of water quality has primarily relied on the use of bacterial indicator methods. However the validity and source-specificity of these methods have been met with increasing reservations for several years now. A relatively recent methodology uses a different chemical biomarker approach using 'sterols', a group of compounds related to the common bio-membrane lipid cholesterol and its derivatives. Sterols can offer an additional diagnostic tool to distinguish and discriminate between sources of faecal contamination in marine, freshwater and estuarine environments in both sediments and the water column. This study investigates for the first time, the degradation of coprostanol and selected faecal sterols in 'natural' sediments from a highly mixed (marine and estuarine) sub-tropical environment following a simulated pollution event (primary effluent); the use of faecal sterols as an additional indicator for determining non-point source sewage discharges at popular anchorages in the Moreton Bay and Gold Coast Broadwater system; and the use of sterol ratios in the determination of the fate and transportation of nutrients from a Sewage Treatment Plant (STP) point-source outlet pipe during plant malfunction. The microcosm degradation experiment revealed that faecal and selected sterols are continually synthesised and degraded over time by auto- and hetero trophic organisms within the sediment matrix. Coprostanol was the only sterol to degrade continually, with only minor fluctuations over a time period of two months. Results from this degradation experiment further revealed a sharp decline of coprostanol within the first week. From this it could be concluded that, without any further addition, external inputs of coprostanol are reduced to background levels within this time period. Therefore, removal of coprostanol after six days was 94% and 73% in mud and sand, respectively. The removal of coprostanol was much higher in mud than sand, reflecting a higher level of microbial activity in muddy sediments for assimilation of sterols. The field study undertaken at popular anchorages in Moreton Bay and the Gold Coast Broadwater revealed extremely low levels of sterols and bacterial indicators over both a spatial and temporal scale consistent with a shallow, oligotrophic, highly dynamic sand dominated system. Even though sterols analysed were found at extremely low levels (mostly in the nano-gram range), they were found to be highly correlated and were successful in identifying an unexpected once off pollution event from a point source at Moreton Bay Island. Other than this one incident, both sterol and bacterial levels were consistently low even when anchorages were at full capacity. Thus, sewage from recreational vessels was found to have very little, if any, effect on the water quality at anchorages in Moreton Bay and Gold Coast Broadwater. The point-source study conducted during a local sewage treatment plant malfunction revealed that even though absolute concentrations of sterols did not change during this event, the distribution of sterols within the samples changed, hence changing the sterol ratios. Further, nutrients (mainly nitrogen) can be transported several kilometres by currents, flocculate out of the water column and settle out into the sediment in areas with low tidal and hydrological flushing. There, the nutrients can cause in situ production of sterols in sediments changing sterol ratios. Overall, this study revealed that analyses of sterol biomarkers have the potential to indicate nutrient inputs (such as nitrogen) as well as sewage, post-hoc pollution events at extremely low levels/high dilutions in coastal sediments.
Thesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Environmental and Applied Science
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Leeming, Rhys, i n/a. "Coprostanol and related sterols as tracers for feacal contamination in Australian aquatic environments". University of Canberra. School of Resource, Environmental & Heritage Sciences, 1996. http://erl.canberra.edu.au./public/adt-AUC20060816.172519.

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Pollution from human and animal faecal waste is a major cause of deteriorating water quality and increased nutrient loads in coastal and inland waterways. Management of this problem depends on knowing which sources of faecal matter are the cause and what is the degree and extent of the pollution. Bacterial indicator organisms have long been the principal method used to test water samples for faecal contamination. However, none of the currently used bacterial indicators on their own are source specific enough to distinguish different sources of faecal matter. The use of faecal sterol biomarkers in conjunction with existing bacterial indicators offers a new way to distinguish sources of faecal contamination. This study investigates the sources of faecal sterols, the relationship of coprostanol to existing bacterial indicators of faecal pollution, the degradation of faecal sterols and the problem of determining the sources of faecal contamination and the distribution of faecal contamination using faecal sterol biomarkers. 5p-Stanols (i.e. faecal sterols) were found to be significant constituents of human, herbivore (i.e. cows, sheep etc.) and pig and cat faeces. Human faeces contained 73 ± 4% coprostanol in relation to the sum of coprostanol and 24-ethylcoprostanol and primary treated effluent contained 86 ± 0.4% coprostanol. Herbivore faeces contained 38 ± 4% coprostanol and 62 ± 4% 24-ethylcoprostanol whereas pig faeces contained 50 � 5% of each compound. Both birds and dogs faeces contained either trace amounts of 5B-stanols or they could not be detected. Notable differences were observed in the abundance of Closthdium perfringens spores between the faeces of birds and domestic pets such as cats and dogs. The above differences were subsequently exploited to distinguish faecal contamination in Lake Tuggerah. An examination of the relationships between coprostanol and bacterial indicator concentrations from several environments revealed that 60 and 400 ng L of coprostanol corresponded to currently defined primary and secondary contact limits for bacteria measured as either thermotolerant coliforms or enterococci in the environment. Four degradation experiments showed faecal sterols and related sterols such as cholesterol decay at similar rates. An induction period was observed in all experiments which meant that simple exponential equations to describe the rate of decay of coprostanol were inadequate; a complimentary log - log transformation of the data was used and the equation: Y = l-Exp(-Exp(time x -0.01 + temp x -0.158 + 3.33)) x 100 was derived where Y equals the predicted percentage of coprostanol remaining over time at a given temperature. In terms of persistence in the environment, Clostridium perfringens spores > coprostanol > enterococci > thermotolerant coliforms. Two field studies were undertaken to highlight the use of faecal sterols. In the Lake Tuggerah study, the results indicated that faecal contamination of receiving waters in the Tuggerah Lakes during rain events was significant, but was not derived from human faecal matter; rather it appears to be principally derived from native birds and, to a lesser extent, domestic pets. In the Derwent Estuary study, based on the distribution of the faecal biomarker coprostanol, the mid estuary and parts of the upper estuary (from Newtown Bay to Taroona), were found to be severely contaminated by sewage. In summary, the use of faecal sterols to trace faecal contamination were found to be an invaluable addition to the tools water managers use to investigate faecal pollution.
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Bull, Ian David. "New molecular methods for tracing natural and anthropogenic inputs to soils and sediments". Thesis, Online version, 1997. http://ethos.bl.uk/OrderDetails.do?did=1&uin=uk.bl.ethos.389168.

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Bishop, C. M. "Monitoring of reproductive condition in birds utilising the noninvasive technique of faecal steroid analysis". Thesis, University of Bristol, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.234591.

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Ogle, Sharron. "Techniques to assist conservation breeding of the babirusa (Babyrousa celebensis)". Thesis, University of Edinburgh, 2010. http://hdl.handle.net/1842/4437.

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With the current rate of loss of biological diversity worldwide estimated at 100- 1000x the natural background rate, solutions are urgently needed to avoid a catastrophic and irreversible loss of species. Conservation breeding is a tool now widely used to assist in maintaining populations in a safe environment until such time as they can be released back into their natural habitat. The babirusa (Babyrousa celebensis) is a threatened species for which conservation breeding is an integral part of its conservation management. Native to a few Indonesian islands, the babirusa is under threat from habitat destruction and hunting and is estimated to have a wild population of only a few thousand. Conservation breeding was established in 1972 with the translocation of animals from Sulawesi to the Surabaya Zoo in Java, Indonesia. It is thought that 1.2 animals founded what is now a worldwide ex-situ population of around 200 individuals. With concerns mounting regarding likely levels of inbreeding and reduced reproductive success, particularly within the European population, this thesis aimed to utilise a number of techniques to assist the conservation breeding programme. Extensive studbook analysis was used to assess typical reproductive parameters for the babirusa in a captive setting; genetic analysis using microsatellite and mitochondrial DNA markers was used to clarify some aspects of the studbook and to estimate the level of variation within and between populations in different geographical areas; and faecal steroid analysis has been used to shed light on the physiological processes underpinning female reproduction. The combined output from these studies has added to our knowledge of this species and its performance in an ex-situ setting, and has now been used to inform future management of the conservation breeding programme.
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Ottosson, Jakob. "Hygiene Aspects of Greywater and Greywater Reuse". Licentiate thesis, KTH, Land and Water Resources Engineering, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-1551.

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Greywater is domestic household wastewater without inputfrom the toilet, i.e. wastewater from sinks, the shower,washing machine and dishwasher in a home. Source separation ofgreywater can be a strategy to enhance recirculation of plantnutrients and/or improve water use. The risk for transmissionof disease when reusing greywater is largely dependent on thecross-contamination by faeces. High levels of faecalindicators, mainly thermotolerant coliform bacteria, have beenreported in greywater, indicating substantial faecal pollution.However, growth of indicator bacteria within the system leadsto an overestimation of thefaecal input and thus the hygienerisk. The faecal input of the greywater in Vibyåsen,Sollentuna, North of Stockholm, was estimated to be 0.04 ±0.02 g faeces person-1 day-1 from the quantification of thefaecal sterol coprostanol, compared to 65 g, 5.2 g and 0.22 gp-1 d-1 using E. coli, enterococci and cholesterolrespectively.

Prevalence of pathogens in the population and the faecalload based on coprostanol concentrations were used to form thebasis of a screening-level quantitative microbial riskassessment (QMRA) that was undertaken for rotavirus, Salmonellatyphimurium, Campylobacter jejuni, Giardia intestinalis andCryptosporidium parvum, looking at the treatment required to bebelow an acceptable level of risk (10-3) for reuse or dischargeof the greywater. The different exposure scenarios simulated–groundwater recharge, direct contact, irrigation andrecreational water–showed that a reduction of 0.7–3.7 log was needed for rotavirus, with the measured level offaecal load in Vibyåsen. The other pathogen of concern wasCampylobacter, where a 2.2 log reduction was needed forgroundwater recharge. The infectious dose of Salmonella is highand the excretion numbers of Giardia cysts and Cryptosporidiumoocysts low, resulting in no treatment requirements for theseorganisms under these circumstances. Pathogen input fromcontaminated food via the kitchen sink had a minor effect onthe microbiological quality of the greywater. Studies on virusoccurrence in greywater as well as validation of the faecalload of greywater at another site would give valuable input forfuture QMRAs.

Greywater treatment efficiency studies, especially on virusremoval, are scarce and more investigations are warranted.Active sludge may not be a suitable technique for greywater dueto the low carbon content in this flow. Chemical precipitationhas the advantage of removing phosphorus as well as virusesefficiently and it is suggested as one possible method fortreating greywater. Otherwise the most common practice forgreywater treatment in Sweden is soil infiltration. However, itis suggested that the recommendations for wastewaterinfiltration also be observed for greywater, despite the lowfaecal load, due to the simulated results on virus reductionneeded.

Key words:greywater, greywater reuse, greywatertreatment, microbial risk assessment, groundwater recharge,irrigation, recreational water, faecal contamination, indicatorbacteria, index organisms, faecal sterols, bacteriophages,enteric pathogens, rotavirus, Salmonella, Campylobacter,Giardia, Cryptosporidium, Legionella

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Oliveira, Luis Fabiano Joaquim de. "Estudo da qualidade do sedimento através de teste de toxicidade e marcadores de esgoto na Enseada de Ubatuba-SP, Brasil. Influência do aumento da população de veraneio". Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/21/21131/tde-13052010-093024/.

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Este estudo teve como objetivo avaliar a toxicidade do sedimento da Enseada de Ubatuba através de testes de toxicidade, utilizando-se fase aquosa e sedimento total antes e depois do aumento da população de veraneio e a contaminação por esgoto urbano. Realizaram-se testes de toxicidade com a água intersticial e interface água/sedimento e larvas de L. variegatus. No testes com sedimento total utilizou-se anfípodo T. viscana. Utilizaram-se as análises de esteróis fecais para verificar a contaminação por esgoto. Foram coletadas em 17 estações. Uma estação do canto direito da Praia do Itaguá foi a única que obteve toxicidade nos três testes na campanha após o aumento da população de veraneio. Para os testes com a fase aquosa, uma estação próxima ao Instituto de Pesca e duas na Praia do Perequê-Açú foram tóxicas nas duas campanhas. Apresentaram contaminação por esgoto e toxicidade para os testes de fase aquosa três estações do canto direito da Praia do Itaguá, uma próxima ao Rio Acaraú, outra em frente ao Rio Grande e também uma na Praia do Perequê-Açú. Uma estação próxima a saída do Rio Grande apresentou indício de contaminação por esgoto, mas não obteve toxicidade na fase aquosa. O aumento da população de veraneio mostrou uma maior toxicidade para os testes com a fase aquosa e uma diminuição do número de sobreviventes de anfípodos. A Enseada de Ubatuba apresentou toxicidade nos testes com fase aquosa em quase todas as estações após o aumento da população, assim deve ser monitorada com freqüência e melhorar a infra-estrutura de saneamento.
The goal of this study was to evaluate the toxicity of the sediment in Ubatuba small Bay through toxicity tests using liquid phase and the total sediment before and after the increase of the floating population and the sewage contamination. The liquid phase was evaluated through toxicity test with interstitial water and water/sediment interface, both with embryos of L. variegatus. The tests with total sediment were utilized amphipod T. viscana. The analyses with chemical faecal steroids marks was utilized for the sewage contamination in the sediment. It was realized samples in 17 stations. One station localized in the right corner of Itaguá beach, was the only one that showed toxicity in the three of the tests in the campaign after the increase of the floating population, two tests with de liquid phase in one station, localized near to Pesca Institute, two stations, localized in the Perequê-Açu beach, was toxic in both campaigns. Contamination by urban sewage and toxicity tests of liquid phase was observed in three stations localized in the right corner of Itaguá beach, other near the mouth of the Acaraú river, and another in front of Grande river and as well one station in the Perequê-Açu beach. One station near the mouth of Grande river had showed indication of contamination by urban sewage, but the liquid phase showed no sign of toxicity. The increase of the floating population showed a high toxicity for the test with liquid phase and a decrease in the number of survives in the amphipods tests. The small bay of Ubatuba showed toxicity in the liquid phase tests in almost of all stations after the increase in the floating population, then the region must be monitored with frequency and it is necessary to improve the structure of the sanitation in the area.
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Höglund, Caroline. "Evaluation of microbial health risks associated with the reuse of source-separated humna urine". Doctoral thesis, KTH, Biotechnology, 2001. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3090.

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Human excreta contain plant nutrients and have the potentialto be used as a fertiliser in agriculture. Urine contributesthe major proportion of the nutrients (N, P and K) in domesticwastewater whereas faeces contribute a smaller amount andinvolves greater health risks if reused due to the possiblepresence of enteric pathogens. Human urine does not generallycontain pathogens that can be transmitted through theenvironment.

Source-separation of urine and faeces is possible by usingurine-separating (or urine-diverting) toilets, available assimple dry toilets or porcelain flush toilets with dividedbowls. The risk for transmission of disease when handling andreusing the urine is largely dependent on thecross-contamination by faeces. In this research, the presenceof human faeces in urine samples was successfully determined byanalysing for faecal sterols. Cross-contamination was evidentin 22% of the samples from urine collection tanks, and in thesequantified to an average (± SD) of 9.1 ± 5.6 mgfaeces per litre urine. Testing for indicator bacteria wasshown to be an unsuitable method for determining faecalcontamination in human urine sinceE. colihad a rapid inactivation in the urine and faecalstreptococci were found to grow within the system.

The fate of any enteric pathogens present in urine iscrucial for the risk for transmission of infectious diseases.Gram-negative bacteria (e.g.SalmonellaandE. coli) were rapidly inactivated (time for 90%reduction, T90<5 days) in source-separated urine at itsnatural pH-value of 9. Gram-positive faecal streptococci weremore persistent with a T90of approximately 30 days. Clostridia sporenumbers were not reduced at all during 80 days. Similarly,rhesusrotavirus andSalmonella typhimuriumphage 28B were not inactivated inurine at low temperature (5°C), whereas at 20°C theirT90-values were 35 and 71 days, respectively.Cryptosporidiumoocysts were less persistent with a T90of 29 days at 4°C. Factors that affect thepersistence of microorganisms in source-separated human urineinclude temperature, pH, dilution and presence of ammonia.

By using Quantitative Microbial Risk Assessment (QMRA), therisks for bacterial and protozoan infections related tohandling and reuse of urine were calculated to be<10-3for all exposure routes independent of the urinestorage time and temperature evaluated. The risk for viralinfection was higher, calculated at 0.56 for accidentalingestion of 1 ml of unstored urine. If the urine was stored at20°C for 6 months the risk for viral infection was reducedto 5.4 × 10-4.

By following recommendations for storage and reuse, whichare dependent on the type of crop to be fertilised, it ispossible to significantly decrease the risk for infections. Sofar, the level of risk that is acceptable is unknown. Theacceptable risk will be one of the main factors determining thefuture utilisation of source-separated human urine inagriculture.

Keywords:urine-separation, urine, wastewater systems,wastewater reuse, recycling, enteric pathogens, faecal sterols,indicator bacteria, hygiene risks, microbial persistence,microbial risk assessment, QMRA, fertiliser, crop.

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Swanepoel, Chantel. "Determination of the quality of environmental water using GC-MS based faecal sterol analysis / Chantel Swanepoel". Thesis, 2014. http://hdl.handle.net/10394/15592.

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Faecal indicator bacteria have traditionally been used in the detection of faecal pollution in water, but due to concerns about the lack of reliability of these indicators, alternative methods have been developed. One of which is the detection of sterols present in human and animal excreta via GC-MS analysis of water in this study. The Szűcs method was used to detect six target sterols (coprostanol, cholesterol, dehydrocholesterol, stigmasterol, β-sitosterol, and stigmastanol) in environmental water samples. An initial study was done by analysing raw sewage and effluent (human faecal sterol biomarkers) and water samples were spiked with excreta from cattle, chickens, horses, pigs, and sheep to determine faecal sterol fingerprints. The method was evaluated for quantitation and differences between the water samples from each species. Following liquid-liquid extraction, silylation and derivatization, samples were analysed by GC-MS. Standard curve assays were linear up to 160ng and the limit for quantification was 20ng. The human faecal sterol biomarker was coprostanol, while herbivore profiles were dominated by terrestrial sterol biomarkers (stigmasterol and stigmastanol). Sterol fingerprints and differences in concentrations of sterols between various animals and between animals and humans occurred, providing the opportunity to determine whether faecal pollution was from humans or from animals. The method proved sensitive enough to evaluate faecal contamination in environmental water. Groundwater was collected from bore-holes and surface water samples were collected from the Baberspan Inland Lake. Physico-chemical parameters analysed indicated that pH for surface water samples was above 6.9. The total dissolved solids (TDS) in groundwater indicated that the water was not suitable for human consumption, but could be used for livestock watering. Surface water electrical conductivity (EC) and inorganic nitrates was too high to be used for irrigational purposes. Nitrates in groundwater were too high to be consumed by humans. In groundwater, the total coliform target water quality range (TWQR) was exceeded at 53% of sites analysed and faecal coliform TWQR were exceeded at 77% sites. Surface water samples complied with TWQR with regards to faecal coliforms for full contact recreational activities and livestock watering. The TWQR for E. coli, with regards to full contact recreational activities, was within a safe range for surface water. Faecal streptococci were found in 85% of groundwater sampling sites. And surface water faecal streptococci counts exceeded the TWQR for full contact recreational activities. There is no TWQR for faecal sterols in water, but concentrations of cholesterol and coprostanol was found at three of the groundwater sites analysed. This indicates faecal contamination from possible animal and human origin. Surface water samples analysed showed that the Harts River water is clean and free of faecal sterols, while the water analysed from the inflow, hotel and outflow, cholesterol eluted, which showed faecal contamination, possibly from animals. Faecal sterol markers could be detected in groundwater and surface water, adding an extra dimension to determining the quality of water systems. An optimization and sensitivity study of the method was done on waste water treatment plant (WWTP) raw sewage and effluent. The WWTP sample analysed form Potchefstroom and Carletonville WWTP yielded all six target sterols in the raw sewage water samples, but no sterols eluted in the effluent samples. The raw sewage water sample taken from the Fochville WWTP yielded all six target sterols as well, however, the effluent yielded an unknown compound as well as cholesterol. An alternative study was done where the effluent sample volume was increased. By increasing the volume of water, one can possibly increase the amount (“load”) of sterols extracted and analysed, resulting in a higher abundance of target sterols. By using the target qualifier ions of the six target sterols, and the GC-TOF/MS software, the target sterols could still be qualitatively determined. Optimal volume for raw sewage is 300 ml water sample as this is enough to yield all 6 target sterols. For optimum water quality monitoring via faecal sterol analysis of effluent and other environmental samples, at least 1L sample volume needs to be collected and analysed. The methods described here can be applied to the analysis of environmental water samples. The technical advantages also make it suitable for routine environmental monitoring of faecal pollution.
MSc (Environmental Sciences), North-West University, Potchefstroom Campus, 2015
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Książki na temat "Faecol sterols"

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Soluble fibre-induced changes in faecal sterol output: A hypocholesterolemic mechanism. Ottawa: National Library of Canada, 1994.

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Części książek na temat "Faecol sterols"

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Knott, Cheryl D., Amy M. Scott, Caitlin A. O’Connell, Tri Wahyu Susanto i Erin E. Kane. "Field and laboratory analysis for non-invasive wildlife and habitat health assessment and conservation". W Conservation Technology, 129–56. Oxford University Press, 2021. http://dx.doi.org/10.1093/oso/9780198850243.003.0007.

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Laboratory methods are increasingly being used in remote field camps, or during mobile field surveys, to aid in wildlife conservation. This chapter explains how field laboratories have allowed for technological advances in sample preparation and preservation, and for both low and high-tech on-site analysis. It highlights how field samples can be used for health and physiological analyses, including measuring the nutritional content of plant foods, assessing hormones, C-peptide, and ketones from urine, examining faecal parasites, and using genetic techniques to determine individual identity, relatedness, and population genetic diversity. We explain how measurements of physiology and health promise to greatly improve our understanding of the relationship between disease prevalence in wild animals and anthropogenic disturbances. The authors’ research with critically endangered wild orangutans in Indonesia provides an illustrative case study, using field laboratories for rapid analysis of orangutan health status, such as assessing indicators of energy balance from urine and parasite prevalence from faeces. In addition, the chapter shows how new information can be gained from field-collected samples by taking advantage of technological advances in laboratory methodology and equipment to determine the nutrient content of foods, measure steroid hormones, and C-peptide and reveal genetic relationships. Understanding how these variables impact wildlife health and viability provides a critical tool for species conservation and helps make long-term research sustainable, provides capacity building opportunities, and forges relationships with local partners. Continued technological advances in the near future should bring more capability into field laboratories, providing data to aid conservation that is easier to obtain and more accessible.
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Juntunen, Kai. "The meaning of stercus in Roman military papyri - dung or human faeces?" W Latrinae: Roman Toilets in the Northwestern Provinces of the Roman Empire, 143–53. Archaeopress Publishing Ltd, 2018. http://dx.doi.org/10.2307/j.ctvndv579.19.

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Rosmarin, Caryn. "The Host-Parasite (Microbe) Relationship". W Tutorial Topics in Infection for the Combined Infection Training Programme. Oxford University Press, 2019. http://dx.doi.org/10.1093/oso/9780198801740.003.0011.

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No and yes. The skin, oropharynx, upper airways, gastrointestinal tract, and lower female genital tract are full of bacteria, with the highest concentration being in the colon and in dental plaque. Overall, humans are made up of slightly more bacterial cells than human cells; about 40 versus 30 trillion respectively. Although much less prominent, fungi and viruses are also present. In addition to these endogenous microbes, humans come into contact with numerous others on a daily basis—they are inhaled, ingested with food and drink, and picked up on the skin from the environment. Some of these remain in and on the human body for periods of time, while others slough off or die. In contrast to this, there are certain areas of the body where microbial agents are not expected to occur under normal circumstances. These are called sterile sites and include: major organs and their surrounding fluids and capsules; blood and body fluids other than faeces and saliva (yes, including urine!); bone, bone marrow, and joint fluid; subcutaneous tissue, fat, muscle, and tendons; the lower respiratory tract; and some of the genital tract. Microbes only enter these protected sterile sites through various breaches in physical and immunological defences. Again— no and yes. This is a question that has posed much debate over the centuries and seems to evolve as understanding of both humans and microbes expands. Early understanding of infectious diseases was based on the idea that the microbe was an aggressor and the host a passive victim. Currently there is a better understanding of the relationship between microbe and host, which is more of a dance than a war. In order to express an understanding of the relationships between host humans and microbes, a language is required that describes this confusing and complex interaction, especially considering that knowledge in this field is still evolving. The bacteria that reside in or on human bodies on a semi- permanent basis are called normal flora, or indigenous microbiome. Each person has a relatively unique set of fairly stable microbes likely determined by early experience, and continued exposures and diets.
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Dolphin, Heather, i Fatima Ahmad. "Bacteriology Diagnostic Methods". W Tutorial Topics in Infection for the Combined Infection Training Programme. Oxford University Press, 2019. http://dx.doi.org/10.1093/oso/9780198801740.003.0015.

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This is summarized in Table 8.1. a) Microscopy— A cell count is performed on sterile fluids and CSF samples using the Neubauer chamber or a similar device. The number of white blood cells (WBC) and red blood cells seen under the microscope are reported as well as the differential WBC count (i.e. the number or percentage of lymphocytes and neutrophils in the sample). A Gram stain is then done and the presence of any organism reported. b) Culture samples are plated onto the appropriate media and streaked out for single colonies as shown. Blood agar is normally used; however, other media are used depending on the site of the specimen, e.g. chocolate agar is used if a fastidious organism is a potential pathogen such as Haemophilus sp.; anaerobic agar for anaerobes; selective agar such as MacConkey can be used on non-sterile specimens to differentiate between the colony types. Plates are incubated for eighteen to forty-eight hours at the correct conditions; most plates being CO2, others at O2 and anaerobically. c) Identification plates are examined for growth. Potentially significant isolates are identified either by MALDI-TOF MS, by API, or other biochemical tests. d) Sensitivities are performed on significant organisms by manual and automated methods. This is summarized in Table 8.2. Selective agar is necessary when isolating pathogens from faeces, although further confirmatory tests are needed. ● Black or colourless colonies on xylose lysine deoxycholate (XLD) or other chromogenic agar plates are tested with oxidase reagent. ● Oxidase negative isolates are identified by MALDI-TOF, API and or biochemically using triple-sugar iron (TSI) tubes. ● Serology is then performed on suspicious isolates and sent to a reference laboratory for confirmation. ● Campylobacter is confirmed by testing grey flat colonies on campylobacter agar with oxidase reagent. Oxidase positive samples are Gram stained and if ‘seagull’-shaped gram-negative bacteria are observed under the microscope, campylobacter is confirmed. The catalase test is a simple biochemical test to differentiate between Staphylococcus species and Streptococcus species, with the use of hydrogen peroxide (H2O2). It tests for the presence of the enzyme catalase which is found in Staphylococcus species.
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Streszczenia konferencji na temat "Faecol sterols"

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Mahajan, Ramit, Vandana Midha, Varun Mehta, Arshdeep Singh, Husanpreet Khattar, Yogesh Gupta, Vikram Narang i Ajit Sood. "IDDF2018-ABS-0218 Efficacy of faecal microbiota therapy in patients with steroid dependent active ulcerative colitis". W International Digestive Disease Forum (IDDF) 2018, Hong Kong, 9–10 June 2018. BMJ Publishing Group Ltd and British Society of Gastroenterology, 2018. http://dx.doi.org/10.1136/gutjnl-2018-iddfabstracts.161.

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