Rozprawy doktorskie na temat „Expression”
Kliknij ten link, aby zobaczyć inne rodzaje publikacji na ten temat: Expression.
Utwórz poprawne odniesienie w stylach APA, MLA, Chicago, Harvard i wielu innych
Sprawdź 50 najlepszych rozpraw doktorskich naukowych na temat „Expression”.
Przycisk „Dodaj do bibliografii” jest dostępny obok każdej pracy w bibliografii. Użyj go – a my automatycznie utworzymy odniesienie bibliograficzne do wybranej pracy w stylu cytowania, którego potrzebujesz: APA, MLA, Harvard, Chicago, Vancouver itp.
Możesz również pobrać pełny tekst publikacji naukowej w formacie „.pdf” i przeczytać adnotację do pracy online, jeśli odpowiednie parametry są dostępne w metadanych.
Przeglądaj rozprawy doktorskie z różnych dziedzin i twórz odpowiednie bibliografie.
1
Testa, Rafael Luiz. "Síntese de expressões faciais em fotografias para representação de emoções". Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/100/100131/tde-31012019-165605/.
Pełny tekst źródłaStreszczenie:
O processamento e a identificação de emoções faciais constituem ações essenciais para estabelecer interação entre pessoas. Alguns transtornos psiquiátricos podem limitar a capacidade de um indivíduo em reconhecer emoções em expressões faciais. De modo a contribuir com a solução deste problema, técnicas computacionais podem ser utilizadas para compor ferramentas destinadas ao diagnóstico, avaliação e treinamento no reconhecimento de tais expressões. Com esta motivação, o objetivo deste trabalho é definir, implementar e avaliar um método para sintetizar expressões faciais que representam emoções em imagens de pessoas reais. Nos trabalhos encontrados na literatura a principal ideia é que a expressão facial da imagem de uma pessoa pode ser reconstituída na imagem de outra pessoa. Este estudo difere-se das abordagens apresentadas na literatura ao propor uma técnica que considera a similaridade entre imagens faciais para escolher aquela que será empregada como origem para a reconstituição. Desta maneira, pretende-se aumentar o realismo das imagens sintetizadas. A abordagem sugerida para resolver o problema, além de buscar as faces mais similares em banco de imagens, faz a deformação dos componentes faciais e o mapeamento das diferenças de iluminação na imagem destino. O realismo das imagens geradas foi mensurado de forma objetiva e subjetiva usando imagens disponíveis em bancos de imagens públicos. Uma análise visual mostrou que as imagens sintetizadas com base em faces similares apresentaram um grau de realismo adequado, principalmente quando comparadas com imagens sintetizadas a partir de faces aleatórias. Além de constituir uma contribuição para a geração de imagens a serem aplicadas em ferramentas de auxílio ao diagnóstico e terapia de distúrbios psiquiátricos, oferece uma contribuição para a área de Ciência da Computação, por meio da proposição de novas técnicas de síntese de expressões faciaisThe ability to process and identify facial emotions are essential factors for an individual\'s social interaction. Some psychiatric disorders can limit an individual\'s ability to recognize emotions in facial expressions. This problem could be confronted by using computational techniques in order to develop learning environments for diagnosis, evaluation, and training in identifying facial emotions. With this motivation, the objective of this work is to define, implement and evaluate a method to synthesize realistic facial expression that represents emotions in images of real people. The main idea of the studies found in the literature is that a facial expression of one persons image can be reenacted in an another persons image. The study differs from the approaches presented in the literature when proposing a technique that considers the similarity between facial images to choose the one that will be used as the origin for reenactment. As a result, we intend to increase the realism of the synthesized images. Our approach to solve the problem, besides searching for the most similar facial components in the image dataset, also deforms the facial elements and maps the differences of illumination in the target image. A visual analysis showed that the images synthesized on the basis of similar faces presented an adequate degree of realism, especially when compared with images synthesized from random faces. The study will contribute to the generation of the images applied to tools for the diagnosis and therapy of psychiatric disorders, and also contribute to the computational field, through the proposition of new techniques for facial expression synthesis
2
Kane, Matthew Patrick. "A Comparison of Micro-Expression Training Methods". ScholarWorks, 2018. https://scholarworks.waldenu.edu/dissertations/5885.
Pełny tekst źródłaStreszczenie:
Micro-expressions are brief facial expressions that last for 500 milliseconds or less and show the true emotional state of an individual when he or she is displaying a false emotional state. There are currently 2 different methods to train individuals to recognize micro-expressions-picture-based and video-based. Numerous organizations use micro-expression training as part of a deception detection program, but little research has been conducted on training outcomes, and no research has investigated the difference between the methods. In this quantitative study based on Darwin's theory of the universality of emotional expression, a control group experimental design was used to determine if there is a difference in training outcomes, as measured by post-training accuracy rates of overall and emotion-specific micro-expression identification, between the 2 current micro-expression training methods and no training. A total of 196 participants recruited from Amazon's Mechanical Turk community were randomly assigned to a picture-based training, video-based training, or no training control group. The online training and post-training test were delivered via a computer-based training platform. MANOVA, ANOVA and t-tests were run to determine the differences between the groups. Results indicated that participants in both picture-based and video-based training groups showed a significant increase in their ability to recognize micro-expressions compared to those in the no training group, but did not differ from each other. The study provides an increased understanding of micro-expression training outcomes that may contribute to the training of numerous law enforcement, security, and human resources professionals.
3
Nascimento, Helvia. "Caracterização da expressão genica de celulas tumorais de pacientes com adenocarcinoma esporadico do colon". [s.n.], 2007. http://repositorio.unicamp.br/jspui/handle/REPOSIP/310956.
Pełny tekst źródłaStreszczenie:
Orientador: Carmen Silvia Passos Lima, Fernando Ferreira CostaTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas
Made available in DSpace on 2018-08-12T11:53:50Z (GMT). No. of bitstreams: 1 Nascimento_Helvia_D.pdf: 1845231 bytes, checksum: 16f227072d706aacb01f06165bd8a553 (MD5) Previous issue date: 2007
Resumo: Os mecanismos moleculares envolvidos na origem do adenocarcinoma de cólon esporádico (ACE) ainda não estão completamente elucidados. Recentemente, o método da análise seriada da expressão gênica (SAGE) foi descrito como eficaz para identificar a expressão total de genes de tipos celulares diversos, mas esta análise não foi realizada em células epiteliais purificadas do ACE moderadamente diferenciado. Nós caracterizamos pelo método SAGE a expressão gênica total de células epiteliais neoplásicas do cólon de um paciente com ACE moderadamente diferenciado (SAGE CC) e de células epiteliais normais do cólon de um paciente com megacólon chagásico (SAGE CN). Foram geradas, após o seqüenciamento automático, 44.004 e 43.570 tags totais das bibliotecas SAGE CC e SAGE CN, representando 16.484 e 13.479 tags únicas, respectivamente. Na comparação entre as bibliotecas, 171 transcritos diferencialmente expressos foram identificados (P< 0,001; expressão diferencial = 5), incluindo 10% de transcritos que podem representar genes não descritos. As expressões de 10 genes diferencialmente expressos foram quantificadas pela reação em cadeia da polimerase em tempo real (qPCR) na amostra de células epiteliais neoplásicas (SAGE CC), com o intuito de validar os resultados obtidos pelo SAGE, e, posteriormente, em amostras de células epiteliais de outros cinco pacientes com o mesmo tipo de doença. As expressões foram concordantes em 80% dos genes (CEACAM6, KLK6, LYZ, PFN1, S100A8, S100A9, VIL2 e ZFHX1B) e discordantes nos demais 20% (PLA1A e ZNF277). As expressões dos genes de interesse, quantificadas pelos dois métodos, foram similares na amostra SAGE CC e nas amostras dos demais pacientes com a doença. Foram observadas expressões anormais de genes envolvidos com a proliferação e diferenciação celular e com a resposta ao stress em células epiteliais neoplásicas. Foram também visualizadas expressões anormais de genes não relacionados com a doença e de genes ainda não identificados. Em conjunto, os nossos resultados podem contribuir para a identificação de genes relacionados com a origem ou a progressão do ACE moderadamente diferenciado e, ainda, para a descoberta de agentes terapêuticos específicos que controlem a proliferação anormal das células neoplásicas.
Abstract: The molecular mechanisms involved in sporadic colon adenocarcinoma (SCA) are still not completely elucidated. Recently, the serial analysis of gene expression (SAGE) method has allowed the global analysis of genes expressed in diverse cellular types but there are no studies in purified epithelial cells of SCA moderately differenciated. We have characterized through SAGE the global gene expression of neoplastic epithelial cells from a SCA moderately differenciated patient (SAGE CC) and normal epithelial cells from a megacolon patient (SAGE CN). After automatic sequencing, a total of 44.004 tags from SAGE CC and 43.570 tags from SAGE CN profiles were generated, representing 16.484 and 13.479 unique tags, respectively. Comparing both profiles, 171 differentially expressed transcripts were identified (P< 0.001; fold = 5), including 10.0% that may represent novel transcripts. The expression of 10 selected genes was further investigated by realtime polymerase chain reaction (qPCR) in the SCA moderately differenciated epithelial cells sample (SAGE CC), with the purpose of to validate the results obtained by the SAGE method, and also in five epithelial cells samples from the same type of SCA patients. Similar expressions were seen in 80% (CEACAM6, KLK6, LYZ, PFN1, S100A8, S100A9, VIL2 e ZFHX1B) and discordant expressions were seen in 20% (PLA1A e ZNF277) of analysed genes. On SAGE CC sample and samples of the SCA patients, all genes presented similar expressions measured by both methods. We observed abnormal expression of genes involved with cell proliferation and differentiation, and with response to stress in neoplastic epithelial cells. Also, were found abnormal expressions of genes not related with the disease and not identified genes. Together, our results may contribute for the identification of genes involved in the origin or progression of SCA moderately differenciated, as well as for the discovery of new therapeutical agents, with specific action on abnormal proliferation of the neoplastic cells.
Doutorado
Ciencias Basicas
Doutor em Clínica Médica
4
Nelson, Nicole L. "A Facial Expression of Pax: Revisiting Preschoolers' "Recognition" of Expressions". Thesis, Boston College, 2011. http://hdl.handle.net/2345/2458.
Pełny tekst źródłaStreszczenie:
Thesis advisor: James A. RussellPrior research showing that children recognize emotional expressions has used a choice-from-array style task; for example, children are asked to find the fear face in an array of several expressions. However, these choice-from-array tasks allow for the use of a process of elimination strategy in which children could select an expression they are unfamiliar with when presented a label that does not apply to other expressions in the array. Across six studies (N = 144), 80% of 2- to 4-year-olds selected a novel expression when presented a target label and performed similarly when the label was novel (such as pax) or familiar (such as fear). In addition, 46% of children went on to freely label the expression with the target label in a subsequent task. These data are the first to show that children extend the process of elimination strategy to facial expressions and also call into question the findings of prior choice-from-array studies
Thesis (PhD) — Boston College, 2011
Submitted to: Boston College. Graduate School of Arts and Sciences
Discipline: Psychology
5
Miao, Yu. "A Real Time Facial Expression Recognition System Using Deep Learning". Thesis, Université d'Ottawa / University of Ottawa, 2018. http://hdl.handle.net/10393/38488.
Pełny tekst źródłaStreszczenie:
This thesis presents an image-based real-time facial expression recognition system that is capable of recognizing basic facial expressions of several subjects simultaneously from a webcam. Our proposed methodology combines a supervised transfer learning strategy and a joint supervision method with a new supervision signal that is crucial for facial tasks. A convolutional neural network (CNN) model, MobileNet, that contains both accuracy and speed is deployed in both offline and real-time frameworks to enable fast and accurate real-time output.
Evaluations for both offline and real-time experiments are provided in our work. The offline evaluation is carried out by first evaluating two publicly available datasets, JAFFE and CK+, and then presenting the results of the cross-dataset evaluation between these two datasets to verify the generalization ability of the proposed method. A comprehensive evaluation configuration for the CK+ dataset is given in this work, providing a baseline for a fair comparison. It reaches an accuracy of 95.24% on JAFFE dataset, and an accuracy of 96.92% on 6-class CK+ dataset which only contains the last frames of image sequences. The resulting average run-time cost for recognition in the real-time implementation is reported, which is approximately 3.57 ms/frame on an NVIDIA Quadro K4200 GPU. The results demonstrate that our proposed CNN-based framework for facial expression recognition, which does not require a massive preprocessing module, can not only achieve state-of-art accuracy on these two datasets but also perform the classification task much faster than a conventional machine learning methodology as a result of the lightweight structure of MobileNet.
6
Carminati, Patricia de Oliveira. "Respostas celulares aos danos causados pelo antitumoral cisplatina em linhagens de fibroblastos humanos normais (MRC-5) e astrocítica (U343 MG-a)". Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/17/17135/tde-21082007-103139/.
Pełny tekst źródłaStreszczenie:
Uma variedade de agentes antitumorais é capaz de induzir danos no material genético e estimular respostas como o bloqueio do ciclo celular, reparo do DNA ou apoptose. A resposta inicial das células é o bloqueio no ciclo em uma tentativa de reparar o dano, no entanto, se esse dano for muito extenso ou comprometer o metabolismo celular, uma cascata de sinalização aciona mecanismos alternativos que inibem a proliferação das células e acionam vias de morte. Os astrocitomas malignos são os tumores cerebrais mais comuns que afetam o sistema nervoso central, compreendendo mais de 60% dos tumores cerebrais primários. O tratamento padrão é a radioterapia seguida de quimioterapia, no entanto, o prognóstico para pacientes portadores desse tipo de câncer ainda continua desanimador. A cisplatina é um agente genotóxico largamente empregado no tratamento de gliomas, além de outros tipos de câncer. Essa droga liga-se ao DNA, formando aductos, os quais levam a um bloqueio na duplicação e na transcrição, podendo induzir apoptose nas células dependendo da extensão do dano. No presente trabalho foram avaliadas as respostas celulares ao tratamento com a cisplatina em linhagem de glioma (U343 MG-a) e em fibroblastos normais transformados por SV40 (MRC-5). Foram avaliadas as respostas em termos de sobrevivência celular, indução de apoptose e expressão gênica em larga escala pela técnica de micro-arranjos de cDNA, sendo esta última realizada somente para a linhagem U343. A cisplatina causou uma redução acentuada na sobrevivência das células MRC-5 (~1%) e U343 (< 1%) após cinco dias de tratamento (teste de sobrevivência celular) com concentrações que variavam de 12,5 a 300 ?M. O tratamento por 24 h com iguais concentrações de cisplatina reduziu a sobrevivência das linhagens em cerca de 20-80% (teste de citotoxicidade). Ambas as linhagens sofreram apoptose após o tratamento com diversas concentrações de cisplatina (12,5, 25 e 50 ?M). A linhagem U343 apresentou uma freqüência máxima de apoptose de 20,4% após o tratamento com 25 ?M de cisplatina por 72 h, enquanto a linhagem MRC-5 apresentou 11,0% de apoptose após 50 ?M de cisplatina por 48 h. Os dados de expressão gênica analisados pelo método de micro-aranjos de cDNA, obtidos 48 h após o tratamento das células U343 com 25 ?M de cisplatina, mostraram genes significativamente (p ?0,05) reprimidos relacionados principalmente com alterações no citoesqueleto (TBCD, RHOA, LIMK2 e MARK1), apoptose ou sobrevivência celular (BCL2-XL, ING1, RHOA, VDP, TIMP2, DYRK3 e NFKBIE), invasão celular ou metástase (LIMK2, TIMP2 e CALU), reparo de DNA (SMC1L1) e metabolismo celular (DYRK3, MARK1, TBCD, LIMK2, VDP e P4HB), entre outros processos. Esses dados apontam para um sério comprometimento da maquinaria celular como um todo após os danos induzidos pela cisplatina. Embora o mecanismo de apoptose justifique cerca de 20% da extensão de morte celular, conforme foi comprovado nos ensaios de apoptose (induzida por 25 ?M de cisplatina), a maior parte das células são eliminadas em conseqüência da ação da droga em vários níveis do metabolismo e manutenção da integridade celular, visto o elevado grau de citotoxicidade da cisplatina, demonstrado nos testes de sobrevivência.A variety of antitumoral agents is capable of inducing DNA damage and eliciting cell cycle arrest, DNA repair or apoptotic responses. The initial response is a cell cycle arrest in an attempt to repair the DNA damage, but under conditions of extensive DNA lesions and high drug cytotoxicity, a signaling cascade triggers alternative mechanisms that inhibit cell proliferation and activate cell death pathways. Astrocytomas are the most common neoplasm of the central nervous system, comprising more than 60% of primary brain tumors. The standard treatment for theses tumors are radiotherapy followed by chemotherapy, however, the prognostic for these patients is still very discouraging. Cisplatin is an efficient DNA-damaging antitumor agent employed for the treatment of various human cancers, including gliomas. This drug binds to DNA, producing diverse types of adducts, which can block replication, transcription, and lead to apoptosis induction. In the present work, we analyzed cellular responses to treatments with the anticancer agent cisplatin in MRC-5 (normal human fibroblasts SV40 transformed) and U343 MG-a (glioma cell line). The responses were evaluated in terms of cell survival, apoptosis induction and profiles of gene expression by the cDNA microarrays method (only for U343 cell line). Cisplatin treatment resulted in a pronounced reduction in MRC-5 cell survival (~ 1%) and U343 (< 1%) after five days of treatment (cell survival test) with several concentrations of cisplatin, ranging from 12.5 to 300 ?M. Following 24h of treatment under similar cisplatin concentrations the survival was reduced at about 20-80% (cytotoxicity test). Both cell lines underwent apoptosis after treatment with different concentrations of cisplatin (12.5; 25 and 50 ?M), but U343 cells presented a maximal frequency of 20.4% apoptosis (25 ?M cisplatin treatment for 72h), while MRC-5 cells presented 11.0% (50 ?M cisplatin treatment for 48h). Analysis of gene expression performed for U343 cells treated with 25 ?M cisplatin for 48h showed several genes that were found significantly (p ? 0,05) down-regulated, most of them related with cytoskeleton alterations (TBCD, RHOA, LIMK2 and MARK1), apoptosis or cell survival (BCL2-XL, ING1, RHOA, VDP, TIMP2, DYRK3 and NFKBIE), cell invasion or metastasis (LIMK2, TIMP2 and CALU), DNA repair (SMC1L1), and cell metabolism (DYRK3, MARK1, TBCD, LIMK2, VDP and P4HB), among others. As a whole, these data demonstrate a serious commitment of the cell machinery after cisplatin-induced cellular damage. About 20% of the cell death corresponds to apoptosis, as was showed by the present assays. However, most of the cells are eliminated by the action of the drug in various levels of the metabolism and maintenance of cell integrity, due to the elevated degree of cisplatin citotoxicity, as demonstrated in cell survival tests.
7
Bunkenburg, Alexander. "Expression refinement". Thesis, University of Glasgow, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360175.
Pełny tekst źródła
8
Santos, Priscila Karla Ferreira dos. "Perfil da expressão gênica de larvas de Tetrapedia diversipes (Hymenoptera: Apidae) em diapausa". Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/41/41131/tde-29032016-135049/.
Pełny tekst źródłaStreszczenie:
A diapausa é um fenômeno amplamente presente nos artrópodes e é considerada como primordial para o sucesso evolutivo da Classe Insecta, pois possibilita a sobrevivência em condições adversas, como estações frias e secas. Sabe-se que durante a diapausa ocorre o silenciamento de muitos genes e que outros são unicamente expressos nesta fase. Embora existam evidências de que o processo da diapausa tenha se mantido conservado durante a evolução das espécies, ainda há lacunas no conhecimento sobre o nível de conservação dos padrões metabólicos. Um bom modelo para se estudar a diapausa é Tetrapedia diversipes, uma espécie bivoltina de abelha solitária. Os indivíduos que nascem na primeira geração seguem o desenvolvimento desde ovo até adulto em tempo bem menor do que aqueles que nascem na segunda geração; estes retardam o desenvolvimento na fase larval. Além disso, essa espécie é de fácil obtenção no seu ambiente natural, pois apresenta alta taxa de nidificação em ninhos-armadilha. O objetivo deste trabalho foi comparar o perfil de expressão de genes entre as larvas da 1ª geração (que não entram em diapausa), larvas da 2ª geração (que entrariam em diapausa) e das larvas em diapausa. Foram identificados 196 genes diferencialmente expressos, destes 87 foram anotados. Muitos destes genes já foram descritos na literatura como relacionados à diapausa em outras espécies, no entanto, o padrão de expressão não é conservado. Os genes aqui identificados foram divididos em cinco grupos: relacionados à desintoxicação celular, cutícula e citoesqueleto, metabolismo de lipídeos e esteróis, ciclo celular e outros genes relacionados à diapausaThe diapause is broadly distributed among the arthropods and has had an important role for the evolutionary success of the Class Insecta, mainly because this process permits insects to explore adverse conditions, such as cold and dry seasons. It is known that there are many genes being silenced and others being uniquely expressed during diapause. And although there are evidences that the diapause process has remained conserved during the evolution of species, it is still not clear how conserved are the metabolic patterns involved in this behavior. Tetrapedia diversipes is a solitary bee and a good model to study diapause. Individuals from the first generation do not enter in diapause and develop faster than individuals from the second generation, which enter in diapause during the winter. Moreover, this species is easy to capture in natural conditions due to the high rate of nesting in trap nests. The aim of this work was to compare the gene expression profile among non-diapause larvae from first and second generation (about to enter diapause) and larvae already in diapause, trough transcriptome data. One hundred ninety-four genes were identified as differentially expressed and 87 of them were annotated. Many of these genes have already been described as related to diapause in others species, but the expression pattern was not conserved. These genes were divided in five groups: related to cellular detoxification, cuticle and cytoskeleton, lipids and steroids metabolism, cell cycle and other genes related to diapause
9
Pucci, Ferdinando. "Gene expression signature and pro-angiogenic function of Tie2-expressing macrophages". Thesis, Open University, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.527443.
Pełny tekst źródła
10
Robinson, Susan Rebecca. "Expression out of expressive physiognomy : physiognomic perception, aesthetic attribution, and art". Thesis, University of Edinburgh, 1998. http://hdl.handle.net/1842/21498.
Pełny tekst źródłaStreszczenie:
This thesis accepts the assumption underlying most theories of expression in art and music in recent decades, that the problem of expression in art is one most adequately handled as a problem of expressive attribution. However, the thesis rejects another assumption these same theories make, that the problem of accounting for descriptions of the form 'the music is sad' forms an isolated study. Instead, the thesis tackles the problem of accounting for expressive qualities attributed to art as part of a wider problem of aesthetic attribution. Chapters One and Two use Fank Sibley's classic distinction between aesthetic and nonaesthetic concepts as the departure point for a wider discussion of aesthetic attribution. Chapter One gives Sibley's discussion an unusually sympathetic reading, defending Sibley against an argument Roger Scruton raises against Sibley's view in the early part of Art and Imagination. Having made the most sympathetic case possible for Sibley's theory, Chapters Two through Five launch into a wider enquiry into the nature of aesthetic perception and aesthetic judgement. In Chapter Two, a final concession is made to Sibley's views when it is suggested that aesthetic judgements employing Sibley's taste concepts constitute strongly perceptual judgements: judgements formed in the course of perception as opposed to judgements reflecting upon the data of perception. Chapter Three continues the enquiry into the nature of aesthetic perception by examining the view expressed sometimes in the writings of Virgil C. Aldrich, that aesthetic awareness amounts to the operation of a distinctive mode of perception, one excluding a physical object awareness of things. The evidence Aldrich provides for his view is examined, and Aldrich's account of representational perception is compared and contrasted with Richard Wollheim's better-known view.
11
Richards, Kevin Stephen. "Insect expression systems : improving intracellular and regulated heterologous gene expression". Thesis, Oxford Brookes University, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.407367.
Pełny tekst źródła
12
Embleton-Lake, Nicola. "Interaction and expression". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ31568.pdf.
Pełny tekst źródła
13
Gotzsch, Josiena. "Managing product expression". Thesis, Brunel University, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274889.
Pełny tekst źródła
14
Rudd, Anthony John. "Scepticism and expression". Thesis, University of Bristol, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.245393.
Pełny tekst źródła
15
Ridley, Aaron. "Expression in music". Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305623.
Pełny tekst źródła
16
Laithier, Gilles. "Expression et sensibilité". Paris 8, 2012. http://www.theses.fr/2012PA084199.
Pełny tekst źródła
17
Preuten, Tobias. "Organellar gene expression". Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2010. http://dx.doi.org/10.18452/16142.
Pełny tekst źródłaStreszczenie:
Zusätzlich zu der eubakteriellen RNA-Polymerase (RNAP) der Plastiden sind im Zellkern von Arabidopsis thaliana drei weitere, phagentypische RNAP kodiert, die jeweils aus nur einer Einheit aufgebaut sind. Die Enzyme RpoTp und RpoTm werden in die Plastiden, bzw. die Mitochondrien transportiert, während RpoTmp in beiden Organellen zu finden ist. Um die Lichtabhängigkeit der RpoT-Gene zu untersuchen, wurde die lichtinduzierte Akkumulation ihrer Transkripte in 7-Tage alten Keimlingen, sowie 3- bzw. 9-Wochen alten Rosettenblättern mittels quantitativer real-time PCR ermittelt. Die entwicklungsabhängige Regulation der RpoT-Transkript-Akkumulation wurde außerdem während der Blattentwicklung analysiert. Zusätzlich wurde der Einfluss des circadianen Rhythmus untersucht. Es stellte sich heraus, dass die Transkriptakkumulation aller drei RpoT-Gene stark lichtinduziert war und nur marginalen circadianen Schwankungen unterlag. In weiteren Versuchen mit verschiedenen Lichtrezeptor-Mutanten und unterschiedlichen Lichtqualitäten wurde der Einfluss multipler Rezeptoren auf den Prozess der Lichtinduktion gezeigt. In den Zellen höherer Pflanzen finden sich drei Genome. Die Biogenese von Chloroplasten und Mitochondrien, sowie lebenswichtige Prozesse, wie Atmung und Photosynthese setzen oftmals die Aktivität von Genen auf mindestens zwei dieser Genome voraus. Eine intrazelluläre Kommunikation zwischen den verschiedenen Genomen ist daher unumgänglich für einen funktionierenden Stoffwechsel der Pflanze. In dieser Arbeit wurde herausgestellt, dass die Zahl mitochondrialer Genkopien in photosynthetisch inaktiven Arabidopsis-Keimlingen drastisch erhöht ist. Bei der Untersuchung des DNA-Gehaltes in Proben, die Altersstufen von 2-Tage alten Keimblättern bis hin zu 37-Tage alten, seneszenten Rosettenblättern umfassten, fand sich ein deutlicher Anstieg der Kopienzahlen in älteren Rosettenblättern. Außerdem unterschieden sich die Kopienzahlen der untersuchten Gene zum Teil erheblich voneinander.In addition to eubacterial-like multi-subunit RNA polymerases (RNAP) localized in plastids and the nucleus, Arabidopsis thaliana contains three phage-like single-unit, nuclear-encoded, organellar RNAPs. The enzymes RpoTp and RpoTm are imported into plastids and mitochondria, respectively, whereas RpoTmp shows dual targeting properties into both organelles. To investigate if expression of the RpoT genes is light-dependent, light-induced transcript accumulation of RpoTm, RpoTp and RpoTmp was analyzed using quantitative real-time-PCR in 7-day-old seedlings as well as in 3- and 9-week-old rosette leaves. To address the question whether RpoT transcript accumulation is regulated differentially during plant development transcript abundance was measured during leaf development. Additionally, effects of the plants circadian rhythm on RpoT transcript accumulation were analyzed. Transcripts of all three RpoT genes were found to be strongly light-induced even in senescent leaves and only marginally influenced by the circadian clock. Further analyses employing different photoreceptor mutants and light qualities revealed the involvement of multiple receptors in the light-induction process. The biogenesis of mitochondria and chloroplasts as well as processes like respiration and photosynthesis require the activity of genes residing in at least two distinct genomes. There have to be ways of intracellular communication between different genomes to control gene activities in response to developmental and metabolic needs of the plant. In this study, it was shown that gene copy numbers drastically increased in photosynthetically inactive Arabidopsis seedlings. Mitochondrial DNA contents in cotyledons and leaves ranging in age from 2-day-old cotyledons to 37-day-old senescent rosette leaves were examined. A common increase in senescing rosette leaves and drastic differences between individual genes were found, revealing the importance of an integrative chondriome in higher plant cells.
18
Sousa, Polyana Ataliba Vasconcelos Medeiros de. "Utilização do sistema SFV em macrófagos". Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-04092015-131041/.
Pełny tekst źródłaStreszczenie:
A raiva é uma antropozoonose e causa a morte de 55 mil pessoas anualmente no mundo. Neste trabalho foi utilizado um sistema genético derivado do Vírus da Floresta de Semliki (SFV) carregando RNA para expressão gênica da glicoproteína do vírus da raiva (RVGP) e da proteína verde fluorescente (GFP), a título de controle do experimento. Lotes de partículas virais de SFV-RVGP e SFV-GFP foram obtidos em células BHK-21 e quantificados através de qRT-PCR. Foram realizados ensaios de infecção tanto com vírus ativado e não ativado em duas linhagens de macrófagos murinos IC-21 e J774A-1. A expressão da proteína RVGP foi avaliada pelo teste de Imunofluorescência Indireta (IFI) e a expressão da proteína GFP analisada por Microscopia de Fluorescência e Citometria de Fluxo em ambas as linhagens. A entrada do vírus SFV em células de mamíferos ocorre após 2 horas da infeção e de acordo com o experimento no qual analisamos a titulação viral do sobrenadante celular, indicaram que até as 72 horas pós infecção a titulação viral se manteve com o mesmo valor inicial, sugerindo que não houve entrada dos vírus na célula por transdução ou por fagocitose.Rabies is a anthropozoonosis and causes the death of 55,000 people worldwide each year. In this work we used a genetic system derived from the Semliki forest virus (SFV) carrying RNA for gene expression of the rabies virus glycoprotein (RVGP) and green fluorescent protein (GFP), by way of experiment control. Plots of viral particles of SFV-RVGP and SFV-GFP were obtained in BHK-21 cells and quantified by qRT-PCR. Infection assays were performed both with activated and not activated in two virus strains of murine macrophages IC-21 and J774A-1. The expression of RVGP protein was assessed by indirect immunofluorescence test (IFI) and the expression of GFP protein analyzed by fluorescence microscopy and flow cytometry in both strains. The SFV entry of viruses into mammalian cells occurs after 2 hours of infection and according to the experiment in which we analyzed the viral titer of the cell supernatant showed that by 72 hours post-infection viral titer remained at the same initial value, suggesting no entry of virus into the cell by transduction or by phagocytosis.
19
Bellintani, Neide Cortizo Andion. "Arte em exposição: um pacto entre a imagem poética e a imagem plástica na obra de Carlos Drummond de Andrade". Programa de Pós-Graduação em Letras e Linguística da UFBA, 2007. http://www.repositorio.ufba.br/ri/handle/ri/11004.
Pełny tekst źródłaStreszczenie:
Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2013-05-14T12:19:29Z
No. of bitstreams: 2
Neide Cortizo Andion Bellintani p2.pdf: 1840181 bytes, checksum: f4264ca2c72c223aef2bd351cd09ee26 (MD5)
Neide Cortizo Andion Bellintani p1.pdf: 456844 bytes, checksum: f9cf97a669af08f22b572e3ecb2d0288 (MD5)Approved for entry into archive by Alda Lima da Silva(sivalda@ufba.br) on 2013-05-16T17:46:17Z (GMT) No. of bitstreams: 2 Neide Cortizo Andion Bellintani p2.pdf: 1840181 bytes, checksum: f4264ca2c72c223aef2bd351cd09ee26 (MD5) Neide Cortizo Andion Bellintani p1.pdf: 456844 bytes, checksum: f9cf97a669af08f22b572e3ecb2d0288 (MD5)
Made available in DSpace on 2013-05-16T17:46:17Z (GMT). No. of bitstreams: 2 Neide Cortizo Andion Bellintani p2.pdf: 1840181 bytes, checksum: f4264ca2c72c223aef2bd351cd09ee26 (MD5) Neide Cortizo Andion Bellintani p1.pdf: 456844 bytes, checksum: f9cf97a669af08f22b572e3ecb2d0288 (MD5) Previous issue date: 2007
Esta pesquisa trata de duas diferentes expressões da Arte, a poética e a plástica. Tem como referência principal a obra Arte em Exposição de Carlos Drummond de Andrade, a qual é constituída de poemas criados pelo autor a partir da observação de reproduções de trabalhos plásticos. Objetiva-se realizar um diálogo entre poemas desta obra com outros do autor, com a sua fortuna crítica e com trabalhos que refletem sobre Poesia e Artes Plásticas. São analisados seis pares de poesias/obras plásticas que fazem parte de Arte em Exposição, buscando situar os poemas em relação a outros da obra de Carlos Drummond de Andrade e comparando as imagens plásticas com os versos que elas motivaram. Cada análise é precedida do poema e da reprodução da obra plástica que o inspirou e encerra-se com algumas informações sobre estas obras e seus autores. Busca-se, através destes instrumentos, delinear uma visão que contemple, as duas expressões – a plástica e a poética, considerando as diferenças entre ambas, mas reconhecendo, nas duas manifestações, importantes contatos. A conjugação das duas expressões artísticas em Arte em Exposição propõe ao observador uma leitura diversa. A mobilidade do olhar (que passeia de uma forma de expressão para a outra) pode torná-lo mais abrangente e as duas imagens, uma vez confrontadas, parecem acrescidas de novos conteúdos. Acredita-se que o exercício de compará-las permite à nossa percepção leituras mais variadas.
Salvador
20
Arcuri, Mariana de Lara Campos [UNESP]. "Identificação e caracterização molecular das lacases de eucalipto (Eucalyptus grandis)". Universidade Estadual Paulista (UNESP), 2017. http://hdl.handle.net/11449/151197.
Pełny tekst źródłaStreszczenie:
Submitted by Mariana de Lara Campos Arcuri null (mariana.arcuri@aluno.ibb.unesp.br) on 2017-07-24T23:48:37Z
No. of bitstreams: 1
Dissertação Final Ficha Catalográfica.pdf: 1462289 bytes, checksum: 6374b4edc59dd6c0ef4dab57c0176073 (MD5)Approved for entry into archive by Luiz Galeffi (luizgaleffi@gmail.com) on 2017-07-26T16:41:02Z (GMT) No. of bitstreams: 1 arcuri_mlc_me_bot.pdf: 1462289 bytes, checksum: 6374b4edc59dd6c0ef4dab57c0176073 (MD5)
Made available in DSpace on 2017-07-26T16:41:02Z (GMT). No. of bitstreams: 1 arcuri_mlc_me_bot.pdf: 1462289 bytes, checksum: 6374b4edc59dd6c0ef4dab57c0176073 (MD5) Previous issue date: 2017-05-24
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
As lacases, p-diphenol-O2-oxidoredutases, são enzimas que desempenham papel fundamental na oxidação de monolignóis durante a biossíntese de lignina, estando, portanto, associadas a processos de crescimento e tolerância a alguns tipos de estresses abióticos. As lacases podem ser encontradas em bactérias, fungos, plantas e insetos. Estudos apontam que as lacases vegetais apresentam comportamento similar às de origem fúngica, atuando de formar complementar à rota de lignificação, em resposta ao estresse oxidativo, promovendo a detoxificação celular. As lacases são geralmente codificadas por famílias multigênicas. Através de análises in silico no genoma de eucalipto, o presente estudo identificou 54 genes codificadores de lacases (denominados EgLAC) que, filogeneticamente, se distribuem em seis diferentes subgrupos. Com base em dados de RNA-Seq, padrões distintos de expressão das lacases identificadas foram observados, sendo algumas enriquecidas em um dado órgão/tecido e outras com expressão não detectável pelo método. Análises de RT–qPCR de alguns genes selecionados com base em um banco de sequências expressas confirmaram, por exemplo, a expressão raiz-específica do gene EgLAC52 bem como as expressões preferenciais em raiz e folha dos genes EgLAC4 e EgLAC32, respetivamente. Em paralelo, a expressão de alguns destes genes em reposta a estresses foi investigado, e alterações na expressão relativa em resposta aos estresses oxidativo e osmótico foram constatadas, sugerindo a participação destas lacases em respostas a estresses abióticos.
Laccases are p-diphenol-O2-oxidoreductases encoded by multigene families widely distributed throughout the plant kingdom. They exhibit important roles in the oxidation of monolignols during lignin biosynthesis and are reported to be functionally involved in plant development, tolerance and response to stress. Apart from plants, laccases can be also found in bacteria, fungi and insects. Here, a genome-wide survey of the eucalyptus genome revealed the presence of 88 putative laccases genes. However, after meticulous analyzes using different approaches, the redundant sequences were discarded and 54 laccases genes (referred as EgLAC) were retrieved. These genes were phylogenetically distributed in six different subgroups. Based on RNA-Seq data, distinct organ/tissue expression patterns of the identified EgLAC genes were ascertained. The vast majority showed organ/tissue-enriched expression, while certain genes exhibited no detectable expression. RT-qPCR analyzes confirmed the organ/tissue expression patterns of a representative set of genes such as, for example, the rootspecific expression of EgLAC52 and the root and leaf preferential expressions of genes EgLAC4 and EgLAC32. Further expression profiling of selected EgLAC genes in response to oxidative and osmotic stresses revealed differences in their relative expression, with some genes being stress-induced. These results suggest that certain laccases might be implicated in abiotic stress responses.
21
Morgan, Daniel Colin. "A Gene Co-Expression Network Mining Approach for Differential Expression Analysis". The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1416989632.
Pełny tekst źródła
22
RODRIGUES, PAULA SALGADO LUCENA. "EXPRESSIVE TALKING HEADS: EXPRESSIVE TALKING HEADS: A STUDY ON SPEECH AND FACIAL EXPRESSION IN VIRTUAL CHARACTERS". PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2002. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=6525@1.
Pełny tekst źródłaStreszczenie:
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFUNDAÇÃO PADRE LEONEL FRANCA
A face humana é interessante e desafiadora acima de tudo pela sua familiaridade. Essencialmente, é a parte do corpo utilizada para reconhecer indivíduos. Assim como a face, a fala é um importante instrumento na forma de comunicação do ser humano. Através da fala é possível externar pensamentos e, muitas vezes, ela indica o estado de ânimo em que uma pessoa se encontra. Juntos, fala e face são os principais elementos de interatividade entre os seres humanos. Contudo, reproduzir com naturalidade e fidelidade as peculiaridades destes dois elementos no universo computacional não é uma tarefa simples, constituindo-se em tópicos de pesquisa em diversas áreas, em particular na animação facial. Entre os diversos tipos de sistemas de animação facial, destacam-se como diretamente relacionados a este trabalho aqueles que envolvem a sincronização da fala de um personagem com a animação da sua face. Sistemas desse tipo são conhecidos como talking head ou talking face. Para o desenvolvimento de um sistema talking head, é necessário identificar as possíveis abordagens para a modelagem dos dois elementos básicos: fala e face. Os modelos utilizados irão influenciar não apenas a maneira como a animação é conduzida, mas a própria forma de interatividade do sistema. Uma contribuição importante deste trabalho é o estudo das possíveis abordagens e a proposta de uma taxonomia para a classificação de sistemas talking head. A partir da taxonomia proposta e fazendo uso de uma determinada abordagem para cada parâmetro analisado, foi desenvolvida uma aplicação que recebe como entrada um texto contendo a fala e anotações de expressividade e gera como saída, em tempo real, a animação de um personagem virtual enunciando o texto de entrada com o áudio e os movimentos faciais sincronizados. O sistema desenvolvido, denominado Expressive Talking Heads, explora a naturalidade da animação facial e ao mesmo tempo busca oferecer ao usuário uma interface com interatividade em tempo real. O Expressive Talking Heads pode ser executado tanto no modo isolado (stand alone) como acoplado a navegadores para a web, tendo sido projetado e desenvolvido com a preocupação de oferecer uma solução independente da plataforma e do sistema operacional utilizados.
The human face is interesting and challenging mainly because of its familiarity. Essentially, it is the part of the human body that is used to recognize individuals. As well as the face, the speech is an important instrument for human communication, allowing the exteriorization of thoughts and the definition of emotions. Together, speech and face are the main elements of interactivity among human beings. However, the natural and faithful reproductions of the pecularities of these elements in the computational universe is not a simple task, constituting topics of the research in the diverse areas, particularly in facial animation. Among the diverse types of facial animation systems developed, those that involve the facial animation of the virtual character combined with speech synchronization are distinguished as directly related to this work. These kinds of systems are known as talking head or talking face. Fot the development of a talking head system, it is necessary to identify the possible approaches for the speech and face modeling. The models used will influence not only the way that the animation is performed, but it will also affect the system´s interactivity. An important contribution of the present master thesis is the study of several possible approaches for the main elements and the proposal of taxonomy for the classification of the talking head systems. From the proposed taxonomy and making use of one approach for each analyzed paramenter, an application was developed that receives as input a text composed by the character´s speech and genus, language and emotion parameters, and it generates as output, in real time, the animation of a virtual character uttering the input text with speech synchronization and expressiveness. The system developed, called Expressive Talking Heads, explores the naturalness of facial animation and it seeks to offer the user a real- time interactivity interface. The Expressive Talking Heads system can run as a stand-alone applicattion or connected to web browsers. It was designed and developed to provide a platform and operating system independent solution.
23
Azevedo, Diego Soares. "Caracterização do perfil de expressão de actina em Aedes aegypti". Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/42/42135/tde-30092014-140546/.
Pełny tekst źródłaStreszczenie:
Nosso estudo se caracterizou em descrever o perfil de expressão de nove genes de actina presentes no genoma do Aedes aegypti ao longo do ciclo de vida do mosquito. Experimentos de qRT-PCR confirmaram a expressão estágio-espécifico de dois genes já caracterizados anteriormente como genes com picos de indução em pupas macho (AeAct-3) e pupas fêmeas (AeAct-4). Outros 2 genes já caracterizados também tiveram seus perfis de expressão confirmados. AeAct-1 se mostrou em baixa expressão e AeAct-2 apresentou picos de indução em larvas de quarto instar. Os demais genes de actina se mostraram expressos em todos os intervalos analisados. Os picos de indução para todos os genes se encontravam ou em pupas ou em larvas de quarto instar. Essa característica se deve ao fato da actina ser uma proteína estrutural, justificando a alta transcrição em larvas tardias e principalmente pupas, fase onde ocorre a metamorfose completa do inseto.Our study was characterized to describe the expression profile of nine actin genes present in the genome of Aedes aegypti throughout the mosquito\'s life cycle. QRT-PCR experiments confirmed the stage-specific expression of two genes as already previously characterized genes with induction peaks of male pupae (AeAct-3) and female pupae (AeAct-4). Two other genes already characterized, also had their expression profiles confirmed. The AeAct-1 showed in low expression and AeAct-2 showed induction peaks in larvae of fourth instar. The remaining actin genes showed expressed in all the analyzed intervals. The induction peaks for all genes were found in pupae or larvae of fourth instar. This characteristic is due to the fact that actin is a structural protein, justifying the high transcription in late larvae and mainly in pupae, stage where the complete metamorphosis of the insect occurs.
24
Silva, Luiz Guilherme Soares da. "Modelo binário para expressão gênica em um embrião de Drosophila melanogaster". Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/100/100132/tde-05092017-163540/.
Pełny tekst źródłaStreszczenie:
Esta dissertação faz uma análise dos resultados de diversas simulações de transcrição usando o modelo binário da expressão gênica regulado externamente. Foram consideradas como variáveis estocásticas o número de moléculas de mRNA e o estado do gene. Neste modelo o gene pode estar no estado ativo (ON) ou reprimido (OFF) e a mudança de estado do gene ocorre a taxas deteminadas para a ativação e para a repressão em cada simulação. O número de moléculas de mRNA é alterado pela transcrição de novas moléculas e pela degradação das moléculas existentes, sendo que esses processos também ocorrem a taxas determinadas. Nas simulações foi utilizado o algoritmo de Gillespie para simulação estocástica exata de reações químicas acopladasThis dissertation analyzes the results of several transcription simulations using the binary model of externally regulated gene expression. The number of mRNA molecules and the state of the gene were considered as stochastic variables. In this model the gene may be in the active state (ON) or repressed state (OFF) and the change of gene state occurs at determined rates in each simulation for activation and for repression. The number of mRNA molecules is changed by the transcription of new molecules and by the degradation of existing molecules at determined rates. These simulations used the Gillespie algorithm for exact stochastic simulation of coupled chemical reactions was used
25
Rodrigues, Gerard A. "Expression of pp60C-SRC in insect cells using a baculovirus expression vector". Thesis, McGill University, 1990. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=59971.
Pełny tekst źródłaStreszczenie:
The early region of polyomavirus encodes three proteins capable of causing tumors in vivo as well as cellular transformation in vitro. Polyomavirus middle T antigen has an associated tyrosine kinase activity due to its interaction with the cellular tyrosine kinase pp60$ sp{ rm c-src}$. To more closely characterize the interaction of these two molecules I have sought to express polyomavirus middle T antigen and pp60$ sp{ rm c-src}$ in insect cells.A variety of transfer vectors have been designed to allow the introduction of foreign genes into the genome of baculoviruses. cDNAs encoding polyomavirus middle T antigen and pp60$ sp{ rm c-src}$ have been cloned into two such vectors that differ in their non-coding upstream sequences. Putative recombinant viruses were selected and examined for the expression of these two gene products. Although permanent recombinant viral stocks were not established, it was demonstrated that pp60$ sp{ rm c-src}$ can be produced in insect cells following transient transfection. Furthermore, this protein was shown to be biologically active as measured in the in vitro immune complex kinase assay. pp60$ sp{ rm c-src}$ produced in insect cells is capable to phosphorylating an exogenous substrate, enolase, in addition to undergoing autophosphorylation.
26
Comber, Julie. "Altered gene expression in glucocorticoid receptor knockout mice : characterization of midkine expression". Thesis, McGill University, 2002. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=33736.
Pełny tekst źródłaStreszczenie:
Glucocorticoid (GC) stimulation of both branching morphogenesis and cytodifferentiation in the developing lung is mediated by the Glucocorticoid Receptor (GR). Seventy-five percent of mice homozygous for a targeted disruption of the GR gene (GR hypo) die shortly after birth due to respiratory failure. Surviving GRhypo mice are normal and fertile. We used Differential Display Reverse Transcription PCR (DD-PCR) and then cDNA microarrays to identify differentially expressed genes in GR hypo compared to wildtype mice. The retinoic acid responsive gene, midkine (MK) was up-regulated in GRhypo mice at fetal day 18 and at neonatal day 1 (in both survivors and non-survivors). At fetal day 16.5, MK was down-regulated in GRhypo mice compared to wildtype. GC treatment up-regulated MK expression in fetal day 20 rat lung Epi cells in primary culture. These results indicate that in the absence of GR, MK is disregulated in the lung: in the pseudoglandular and canalicular stage, MK is down-regulated, but by the saccular stage, and then shortly after birth, the absence of GR results in up-regulation of MK expression. Our findings suggest that down-regulation of the growth factor MK in late gestation is essential to normal terminal differentiation of the fetal lung.
27
Wallace, Carrie T. "The influence of endogenous expression of Tal-1 on apoptotic gene expression". Muncie, Ind. : Ball State University, 2008. http://cardinalscholar.bsu.edu/390.
Pełny tekst źródła
28
Lemons, Derek Scott. "Gene expression and evolution". Diss., [La Jolla] : University of California, San Diego, 2010. http://wwwlib.umi.com/cr/ucsd/fullcit?p3397172.
Pełny tekst źródłaStreszczenie:
Thesis (Ph. D.)--University of California, San Diego, 2010.Title from first page of PDF file (viewed March 23, 2010). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 98-111).
29
Chung, Hweawon. "The expression of emotions /". Link to online version, 2006. https://ritdml.rit.edu/dspace/handle/1850/2476.
Pełny tekst źródła
30
Tekin, Elif. "Dynamic Expression Of Three". Master's thesis, METU, 2011. http://etd.lib.metu.edu.tr/upload/12613685/index.pdf.
Pełny tekst źródłaStreszczenie:
RNA-binding proteins (RBP) shuttle between cellular compartments either constitutively or in response to stress and regulate localization, translation and turn over of mRNAs. In our laboratory, cytosolic proteome map of Phanerochaete chrysosporium was established and upon Pb exposure, the changes in cytosolic protein expressions were determined. The identified RBPs were a newly induced polyadenylate-binding protein (RRM superfamily) as well as two up-regulated proteins, namely splicing factor RNPS1 and ATP-dependent RNA helicase, all being very important candidates of post-transcriptional control in response to stress. This finding inspired us to conduct Real Time PCR studies in order to have a better understanding of the changes in the expression of corresponding genes at mRNA level in response to Pb exposure, thus the present study aims at examining the effect of lead exposure on the transcript levels of the genes coding for ATP-dependent RNA helicase, splicing factor RNPS1 and polyadenylate binding protein. As shown via expression analysis based on Real Time PCR, the mRNA level of splicing factor RNPS1 showed 2.68, 2.62 and 4.86 fold increases in a dose-dependent manner when the cells were grown for 40 h in the presence of 25, 50 and 100 µM Pb, repectively. ATP-dependent RNA helicase mRNA level showed no significant increase in response to 25 µ
M Pb exposure while increased 2 and 1.84 fold in response to 50 and 100 µ
M Pb, respectively. Polyadenylate binding protein mRNA levels revealed no significant increase when exposed to 25, 50 and 100 µ
M Pb. As to the mRNA dynamics as a function of duration of lead exposure, the mRNA level of this protein showed 2.54-fold increase upon 1 h exposure to 100 µ
M Pb. Splicing factor RNPS1 mRNA level showed a significant increase of 19.22 fold at 2nd h of 50 µ
M Pb exposure. Expression level of ATP-dependent RNA helicase was not affected by the time of exposure to Pb.
31
Baltrušaitis, Tadas. "Automatic facial expression analysis". Thesis, University of Cambridge, 2014. https://www.repository.cam.ac.uk/handle/1810/245253.
Pełny tekst źródłaStreszczenie:
Humans spend a large amount of their time interacting with computers of one type or another. However, computers are emotionally blind and indifferent to the affective states of their users. Human-computer interaction which does not consider emotions, ignores a whole channel of available information. Faces contain a large portion of our emotionally expressive behaviour. We use facial expressions to display our emotional states and to manage our interactions. Furthermore, we express and read emotions in faces effortlessly. However, automatic understanding of facial expressions is a very difficult task computationally, especially in the presence of highly variable pose, expression and illumination. My work furthers the field of automatic facial expression tracking by tackling these issues, bringing emotionally aware computing closer to reality. Firstly, I present an in-depth analysis of the Constrained Local Model (CLM) for facial expression and head pose tracking. I propose a number of extensions that make location of facial features more accurate. Secondly, I introduce a 3D Constrained Local Model (CLM-Z) which takes full advantage of depth information available from various range scanners. CLM-Z is robust to changes in illumination and shows better facial tracking performance. Thirdly, I present the Constrained Local Neural Field (CLNF), a novel instance of CLM that deals with the issues of facial tracking in complex scenes. It achieves this through the use of a novel landmark detector and a novel CLM fitting algorithm. CLNF outperforms state-of-the-art models for facial tracking in presence of difficult illumination and varying pose. Lastly, I demonstrate how tracked facial expressions can be used for emotion inference from videos. I also show how the tools developed for facial tracking can be applied to emotion inference in music.
32
Humphries, Clare Ruth. "Gene expression in schizophrenia". Thesis, Imperial College London, 1997. http://hdl.handle.net/10044/1/7770.
Pełny tekst źródła
33
Mikheeva, Olga. "Perceptual facial expression representation". Thesis, KTH, Robotik, perception och lärande, RPL, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-217307.
Pełny tekst źródłaStreszczenie:
Facial expressions play an important role in such areas as human communication or medical state evaluation. For machine learning tasks in those areas, it would be beneficial to have a representation of facial expressions which corresponds to human similarity perception. In this work, the data-driven approach to representation learning of facial expressions is taken. The methodology is built upon Variational Autoencoders and eliminates the appearance-related features from the latent space by using neutral facial expressions as additional inputs. In order to improve the quality of the learned representation, we modify the prior distribution of the latent variable to impose the structure on the latent space that is consistent with human perception of facial expressions. We conduct the experiments on two datasets and the additionally collected similarity data, show that the human-like topology in the latent representation helps to improve the performance on the stereotypical emotion classification task and demonstrate the benefits of using a probabilistic generative model in exploring the roles of latent dimensions through the generative process.Ansiktsuttryck spelar en viktig roll i områden som mänsklig kommunikation eller vid utvärdering av medicinska tillstånd. För att tillämpa maskininlärning i dessa områden skulle det vara fördelaktigt att ha en representation av ansiktsuttryck som bevarar människors uppfattning av likhet. I det här arbetet används ett data-drivet angreppssätt till representationsinlärning av ansiktsuttryck. Metodologin bygger på s. k. Variational Autoencoders och eliminerar utseende-relaterade drag från den latenta rymden genom att använda neutrala ansiktsuttryck som extra input-data. För att förbättra kvaliteten på den inlärda representationen så modifierar vi a priori-distributionen för den latenta variabeln för att ålägga den struktur på den latenta rymden som är överensstämmande med mänsklig perception av ansiktsuttryck. Vi utför experiment på två dataset och även insamlad likhets-data och visar att den människolika topologin i den latenta representationen hjälper till att förbättra prestandan på en typisk emotionsklassificeringsuppgift samt fördelarna med att använda en probabilistisk generativ modell när man undersöker latenta dimensioners roll i den generativa processen.
34
Fischer, Heléne. "Gene expression in carcinogenesis /". Stockholm, 2001. http://diss.kib.ki.se/2001/91-628-4961-1/.
Pełny tekst źródła
35
Murphy, Christopher Eric. "Reconstructing paradigms of expression". Click here to access thesis, 2007. http://www.georgiasouthern.edu/etd/archive/spring2007/christopher_e_murphy/Murphy_Christopher_E_200701_mfa.pdf.
Pełny tekst źródłaStreszczenie:
Thesis (M.F.A.)--Georgia Southern University, 2007."A thesis submitted to the Graduate Faculty of Georgia Southern University in partial fulfillment of the requirements for the degree Master of Fine Arts." In Art, under the direction of Patricia J. Walker. ETD. Electronic version approved: May 2007. Includes bibliographical references (p. 37-38)
36
Person, Gretchen E. "The expression of joy". Theological Research Exchange Network (TREN), 1990. http://www.tren.com.
Pełny tekst źródła
37
Chandrapati, Srivardhan. "Multi-modal expression recognition". Thesis, Manhattan, Kan. : Kansas State University, 2008. http://hdl.handle.net/2097/762.
Pełny tekst źródła
38
Hornan, Daniel Mark. "Human macular gene expression". Thesis, University College London (University of London), 2005. http://discovery.ucl.ac.uk/1444745/.
Pełny tekst źródłaStreszczenie:
The human macula is essential for precise vision. It contains many more cone photoreceptors than the peripheral retina, especially in the fovea. Cones are known to express specific opsins and other proteins that form part of the phototransduction cascade. However, relatively little is known about retinal macular gene expression compared with the rod-rich peripheral retina. I obtained human donor eyes and used foveo-macular and macular punches and sections of peripheral retina to study differential gene expression. I combined multiple microarray experiments with quantitative PCR, statistical, and bioinformatic analyses. I identified several known and previously unidentified retinal genes that are more abundant in the macula. I went on to characterize proteins encoded by histone deacetylase 9 and the morpheus gene family. Both were expressed in the human macula, especially in the photoreceptors. Several other genes also provided insight into the mechanisms of precise vision and its maintenance. Genes identified by this approach are excellent candidates for macular disease.
39
Tebbutt, Scott James. "Pollen-specific gene expression". Thesis, University of East Anglia, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334705.
Pełny tekst źródła
40
Al-Nbaheen, May Salem. "Liver specific gene expression". Thesis, University of Bath, 2002. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.760790.
Pełny tekst źródła
41
Herbert, John Matthew Jeff. "Endothelial cell gene expression". Thesis, University of Birmingham, 2013. http://etheses.bham.ac.uk//id/eprint/3803/.
Pełny tekst źródłaStreszczenie:
Tumour angiogenesis is a vital process in the pathology of tumour development and metastasis. Targeting markers of tumour endothelium provide a means of targeted destruction of a tumours oxygen and nutrient supply via destruction of tumour vasculature, which in turn ultimately leads to beneficial consequences to patients. Although current anti-angiogenic and vascular targeting strategies help patients, more potently in combination with chemo therapy, there is still a need for more tumour endothelial marker discoveries as current treatments have cardiovascular and other side effects. For the first time, the analyses of in-vivo biotinylation of an embryonic system is performed to obtain putative vascular targets. Also for the first time, deep sequencing is applied to freshly isolated tumour and normal endothelial cells from lung, colon and bladder tissues for the identification of pan-vascular-targets. Integration of the proteomic, deep sequencing, public cDNA libraries and microarrays, delivers 5,892 putative vascular targets to the science community. These analyses identify Endothelial Specific Molecule 1 as a pan vascular target and lysyl oxidase-like 2 as putative novel vascular target. It is envisioned vascular targets and angiogenesis genes in this data will destroy or inhibit tumour vessel growth without the side effects manifest with current clinical regimens.
42
Skelley, James P. (James Paul). "Experiments in expression recognition". Thesis, Massachusetts Institute of Technology, 2005. http://hdl.handle.net/1721.1/37063.
Pełny tekst źródłaStreszczenie:
Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2005.Includes bibliographical references (p. 39-41).
Despite the significant effort devoted to methods for expression recognition, suitable training and test databases designed explicitly for expression research have been largely neglected. Additionally, possible techniques for expression recognition within an Man-Machine-Interface (MMI) domain are numerous, but it remains unclear what methods are most effective for expression recognition. In response, this thesis describes the means by which an appropriate expression database has been generated and then enumerates the results of five different recognition methods as applied to that database. An analysis of the results of these experiments is given, and conclusions for future research based upon these results is put forth.
by James P. Skelley.
M.Eng.
43
Li, Yan 1978 July 15. "Gene expression array simulator". Thesis, Massachusetts Institute of Technology, 2002. http://hdl.handle.net/1721.1/87263.
Pełny tekst źródłaStreszczenie:
Thesis (M.Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, June 2002."May 10, 2002.
Includes bibliographical references (leaf 141).
by Yan Li.
M.Eng.
44
Riek, Laurel Dawn. "Expression synthesis on robots". Thesis, University of Cambridge, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609675.
Pełny tekst źródła
45
Garcia, Cristian. "A Vehicle of Expression". Scholarship @ Claremont, 2016. http://scholarship.claremont.edu/cmc_theses/1271.
Pełny tekst źródłaStreszczenie:
This senior thesis studies the evolution and ideals of several populations in Los Angeles through the lens of car culture. The automobile is a symbol of expression and upon analyzing it, a great deal can be revealed about its owner. Los Angeles is home to the hot rodding, lowriding, and import tuning car movements. All three major car cultures were born from a marginalized youth population. The three movements shed light on the sentiments and assimilation process of the various ethnic communities that created the car culture. This essay will show how each movement not only influenced one another, but also the car industry as a whole. Additionally, this essay examines how advancements in technology have led the current millennial generation to form a mass youth culture. The mass youth culture of the present day is much different than the young populations of past time periods, and that is reflected when analyzing modern day car culture.
46
Humphries, Carl. "Musical expression and performance". Thesis, University of Southampton, 2006. https://eprints.soton.ac.uk/192757/.
Pełny tekst źródłaStreszczenie:
This study examines the philosophical question of how it is possible to appreciate music aesthetically as an expressive art form. First it examines a number of general theories that seek to make sense of expressiveness as a characteristic of music that can be considered relevant to our aesthetic appreciation of the latter. These include accounts that focus on resemblances between music and human behaviour or human feelings, on music's powers of emotional arousal, and on various ways in which music may be imaginatively construed by listeners. It argues that none of these are entirely satisfactory. Then it proposes an alternative account, focusing on what is involved when our appreciation of music as an expressive art is informed by our awareness of it as something that is expressively interpreted in performance. It is claimed that this offers the basis for a better understanding of at least some aspects of expressiveness in music and its relevance to aesthetic appreciation.
47
Li, Jingting. "Facial Micro-Expression Analysis". Thesis, CentraleSupélec, 2019. http://www.theses.fr/2019CSUP0007.
Pełny tekst źródłaStreszczenie:
Les micro-expressions (MEs) sont porteuses d'informations non verbales spécifiques. Cependant, en raison de leur nature locale et brève, il est difficile de les détecter. Dans cette thèse, nous proposons une méthode de détection par reconnaissance d'un motif local et temporel de mouvement du visage. Ce motif a une forme spécifique (S-pattern) lorsque la ME apparait. Ainsi, à l'aide de SVM, nous distinguons les MEs des autres mouvements faciaux. Nous proposons également une fusion spatiale et temporelle afin d'améliorer la distinction entre les MEs (locaux) et les mouvements de la tête (globaux). Cependant, l'apprentissage des S-patterns est limité par le petit nombre de bases de données de ME et par le faible volume d'échantillons de ME. Les modèles de Hammerstein (HM) est une bonne approximation des mouvements musculaires. En approximant chaque S-pattern par un HM, nous pouvons filtrer les S-patterns réels et générer de nouveaux S-patterns similaires. Ainsi, nous effectuons une augmentation et une fiabilisation des S-patterns pour l'apprentissage et améliorons ainsi la capacité de différencier les MEs d'autres mouvements. Lors du premier challenge de détection de MEs, nous avons participé à la création d’une nouvelle méthode d'évaluation des résultats. Cela a aussi été l’occasion d’appliquer notre méthode à longues vidéos. Nous avons fourni le résultat de base au challenge.Les expérimentions sont effectuées sur CASME I, CASME II, SAMM et CAS(ME)2. Les résultats montrent que notre méthode proposée surpasse la méthode la plus populaire en termes de F1-score. L'ajout du processus de fusion et de l'augmentation des données améliore encore les performances de détectionThe Micro-expressions (MEs) are very important nonverbal communication clues. However, due to their local and short nature, spotting them is challenging. In this thesis, we address this problem by using a dedicated local and temporal pattern (LTP) of facial movement. This pattern has a specific shape (S-pattern) when ME are displayed. Thus, by using a classical classification algorithm (SVM), MEs are distinguished from other facial movements. We also propose a global final fusion analysis on the whole face to improve the distinction between ME (local) and head (global) movements. However, the learning of S-patterns is limited by the small number of ME databases and the low volume of ME samples. Hammerstein models (HMs) are known to be a good approximation of muscle movements. By approximating each S-pattern with a HM, we can both filter outliers and generate new similar S-patterns. By this way, we perform a data augmentation for S-pattern training dataset and improve the ability to differentiate MEs from other facial movements. In the first ME spotting challenge of MEGC2019, we took part in the building of the new result evaluation method. In addition, we applied our method to spotting ME in long videos and provided the baseline result for the challenge. The spotting results, performed on CASME I and CASME II, SAMM and CAS(ME)2, show that our proposed LTP outperforms the most popular spotting method in terms of F1-score. Adding the fusion process and data augmentation improve even more the spotting performance
48
Davydiuk, T. O. "Fashion as self-expression". Thesis, Київський національний університет технологій та дизайну, 2018. https://er.knutd.edu.ua/handle/123456789/11386.
Pełny tekst źródła
49
Noda, Ryuji. "Seeking ways of expression". The Ohio State University, 1995. http://rave.ohiolink.edu/etdc/view?acc_num=osu1322066048.
Pełny tekst źródła
50
Jang, Lee-Soo. "Search for self expression". The Ohio State University, 1997. http://rave.ohiolink.edu/etdc/view?acc_num=osu1327680796.
Pełny tekst źródła