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Carrillo-Lopez, Luis M., Bianka Y. Cruz-Garibaldi, Mariana Huerta-Jimenez, Ivan A. Garcia-Galicia i Alma D. Alarcon-Rojo. "The Physicochemical, Microbiological, and Structural Changes in Beef Are Dependent on the Ultrasound System, Time, and One-Side Exposition". Molecules 27, nr 2 (15.01.2022): 541. http://dx.doi.org/10.3390/molecules27020541.
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The effect of high-intensity ultrasound (HIU) system (bath, 37 kHz and 90 W/cm2; or probe, 24 kHz and 400 W) and application time (25 or 50 min, one-side exposition) on the properties of bovine Longissimus lumborum after 7 d of storage at 4 °C was studied. The bath system significantly increased the lightness of the muscle, while other color parameters (a*, b*, hue, and chroma) were not different from the control. The water holding capacity and shear force decreased significantly (3.1–5% and 0.59–0.72 kgf, respectively) in sonicated meat independently of the system, favoring the tenderization of the muscle after storage. Microstructural changes observed in the HIU-exposed surface provided evidence of a higher area of interfibrillar spaces (1813 vs. 705 µm2 in the control), producing tenderization of the muscle, compared with the control. HIU significantly increased counts of total aerobic and coliform bacteria, especially after 50 min of ultrasonication. HIU also increased lactic acid bacterial counts in the bath system. Single-sided muscle exposition to ultrasound may produce sufficient significant changes in muscle properties, which could decrease long treatment times that would be needed for the exposition of both sides. HIU in bath systems increases tenderness by modifying meat ultrastructure, with no significant changes in physicochemical parameters. Nevertheless, microbiological quality may need to be considered during the process due to a slight increase in bacterial counts.
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Schnelle, Daniel, Frank-Jürgen Weinreich, Janek Kibat i Marc A. Reymond. "A new ex vivo model for optimizing distribution of therapeutic aerosols: the (inverted) bovine urinary bladder". Pleura and Peritoneum 2, nr 1 (1.03.2017): 37–41. http://dx.doi.org/10.1515/pp-2017-0006.
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AbstractBackgroundDevelopment of Pressurized IntraPeritoneal Aerosol Chemotherapy (PIPAC) requires adequate preclinical models.MethodsThe model should be easy to use, reproducible and cost-effective. It should have a volume similar to the human abdominal cavity, and an oval shape. The inner surface should be lined with serosa. The model should allow pharmacological and biological analysis, including histology. No living animals should be used.ResultsThe fresh urinary bladder is explanted from an adult bovine in the slaughterhouse. A 4-cm incision is performed into the bladder neck. The bladder can be inverted through the incision, which allows exposition of the serosa on its inner side. A balloon trocar is inserted through the incision and a ligature placed, ensuring full tightness. The therapeutic capnoperitoneum is installed. The bovine bladder has a volume somewhat smaller (2–3 L) than the human abdominal cavity (3–5 L). Costs are minimal. There is no significant bacteriological contamination. Manipulation is simple.ConclusionsThe (inverted) bovine urinary bladder is an innovative and versatile ex vivo model for optimizing drug delivery with therapeutic aerosols both onto the mucosa or the serosa. This model can be used for pharmaceutical Quality-by-Design approaches and will replace a large number of experiments in the animal.
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Grigoryan, Svetlana V. "STUDY OF SPECTRAL CHARACTERISTICS OF BOVINE SERUM ALBUMIN UNDER THE EFFECT OF MILLIMETER RANGE ELECTROMAGNETIC WAVES". Proceedings of the YSU B: Chemical and Biological Sciences 58, nr 2 (264) (24.06.2024): 126–32. http://dx.doi.org/10.46991/pysu:b.2024.58.2.126.
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The study of the effect of millimeter range electromagnetic waves (MM EMW) on bovine serum albumin (BSA) structure at the frequencies both resonant (51.8 GHz) and non-resonant (41.8 GHz) for water has been conducted. The absorption and fluorescence spectra of non-irradiated and irradiated samples of physiological solution and BSA with exposition 60 min were obtained. It was revealed that the spectral characteristics of physiological solution did not change under the effect of MM EMW, while the analogous characteristics of BSA change relevantly. It was also shown that the absorption spectra of the irradiated samples significantly differ from those of non-irradiated protein.
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Rios, G. L., G. G. Kaiser, N. C. Mucci i R. H. Alberio. "111 EFFECT OF ETHYLENE GLYCOL IN VITRIFICATION MEDIUM ON BOVINE OOCYTE ACTIVATION AND IN VITRO EMBRYO PRODUCTION". Reproduction, Fertility and Development 22, nr 1 (2010): 214. http://dx.doi.org/10.1071/rdv22n1ab111.
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In this study the effect of increasing ethylene glycol (EG) concentrations in the vitrification media (VM) and its relation with oocyte activation and in vitro embryo production were evaluated. Cumulus-oocytes complexes (COC) were matured in vitro for 22 h and then partially denuded by gently pipetting with hyaluronidase, and randomly assigned to 4 treatments: T1 = control group; T2 = COC exposed to 10% EG + 10% DMSO (VM1), 20% EG + 20% DMSO (VM2); T3 = 15% EG + 5% DMSO (VM1), 30% EG + 10% DMSO (VM2); T4 = 20% EG + 0% DMSO (VM1), 40% EG + 0% DMSO (VM2). Exposition to VM1 and VM2 lasted 3 min and 30 s, respectively. After treatment COC were incubated in maturation medium up to 24 h. In Experiment 1 COC were cultured for 24 h in fertilization TALP supplemented with 50 μg mL-1 of heparin, then completely denuded and cultured 24 h in CR1-aa. After this, oocytes were stained with bisbenzimide (Hoechst 33342) and the number of nuclei and cells were recorded. Structures presenting 2 cells or 2 nuclei were considered as activated oocytes. In Experiment 2 matured COC exposed to cryoprotectants as in Experiment 1 were fertilized and cultured for 6 days as previously described (Mucci et al. 2006). Variables analyzed included oocyte activation, cleavage rate at 48 h, and percentage of viable embryos at Day 7. Data were analyzed by PROC GENMOD (SAS Institute Inc., Cary, NC, USA). Activated oocytes percentage did not differ between EG concentrations in VM and were higher (T2, 24.7%, n = 101; T3, 25.0%, n = 96; T4, 30.2%, n = 119) compared with controls (T1, 9.8%, n = 61). In Experiment 2 no differences were found in cleavage rates (T1, 81.9%, n = 68; T2, 87%, n = 67; T3, 85.9%, n = 61; T4, 84.2%, n = 64) and Day 7 percentage of viable embryos (T1, 34.9%, n = 29; T2, 28.6%, n = 22; T3, 26.8%, n = 19; T4, 27.6%, n = 21) in treated COC. The exposition of COC to cryoprotectants per se could trigger oocyte activation in the range of 10 to 40%. Thanks toAdriana Cano (Instituto Nacional de Tecnología Agropecuaria) for contributions in statistical analysis.
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Lentini, Alessandro, Laura Falasca, Francesco Autuori i Luciana Dini. "The simultaneous exposition of galactose and mannose-specific receptors on rat liver macrophages is developmentally regulated". Bioscience Reports 12, nr 6 (1.12.1992): 453–61. http://dx.doi.org/10.1007/bf01122033.
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We studied the simultaneous binding of galactose and mannose-exposing ligands in sinusoidal rat liver cells during development and aging. The galactose-specific receptors were visualized using 17 nm diameter colloidal gold particles coupled with Lactosylated bovine serum albumine (LacBSA), while mannose-specific receptors were localized by means of 5 nm diameter particles adsorbed with mannan. We observed the presence of four different classes of Kupffer cells in relation to the ligands bound. The percentage of each group of Kupffer cells varied in relation to the age of the subject from which the sample was taken. There were few double-labelled cells in the livers from newborn rats, with numbers increasing with age to adulthood, and decreasing again in the older animals. Cells without labelling were in the majority after birth, but they decreased in number up to adulthood and increased again during subsequent aging. The numbers of single-labelled cells did not change significantly during liver maturation. We hypothesize that the exposition of galactose and mannose-specific receptorial systems is regulated by developmental conditions.
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Gorza, Filipe D. S., Graciela C. Pedro, Tarquin F. Trescher, Romário J. da Silva, Josmary R. Silva i Nara C. de Souza. "Morphological Analysis and Interaction of Chlorophyll and BSA". BioMed Research International 2014 (2014): 1–6. http://dx.doi.org/10.1155/2014/872701.
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Interactions between proteins and drugs, which can lead to formation of stable drug-protein complexes, have important implications on several processes related to human health. These interactions can affect, for instance, free concentration, biological activity, and metabolism of the drugs in the blood stream. Here, we report on the UV-Visible spectroscopic investigation on the interaction of bovine serum albumin (BSA) with chlorophyll (Chl) in aqueous solution under physiological conditions. Binding constants at different temperatures—obtained by using the Benesi-Hildebrand equation—were found to be of the same order of magnitude (~104 M−1) indicating low affinity of Chl with BSA. We have found a hyperchromism, which suggested an interaction between BSA and Chl occurring through conformational changes of BSA caused by exposition of tryptophan to solvent. Films from BSA and Chl obtained at different Chl concentrations showed fractal structures, which were characterized by fractal dimension calculated from microscopic image analysis.
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García-Galicia, Iván A., Christopher Estepp, Mariana Huerta-Jiménez, Maria F. Melchor-Ramírez, Luis M. Carrillo-López, Einar Vargas-Bello-Pérez i Alma D. Alarcón-Rojo. "Physicochemical Properties and Young Adult Consumer Preference of Dry-Aged Beef after High-Intensity Ultrasonication". Processes 10, nr 10 (21.10.2022): 2145. http://dx.doi.org/10.3390/pr10102145.
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This study aimed to determine the effect of high-intensity ultrasound (HIU. F = 37 kHz, I = 28W/cm2, bath for 30 min, 5 °C) on physicochemical characteristics and sensorial preference of seven aged (23 d ageing) bovine muscles (L. dorsi lumborum, L. dorsi thoracis, Psoas major, Semimembranosus, Biceps femoris, Rectus femoris, and Gluteus medius). Muscles were randomly distributed in two treatments: with and without ultrasonication. Colour (L*, a*, b* and C*), water-holding capacity (WHC), and shear force (N) were determined before and after simulated retail display (SRD) in modified atmosphere packing (MAP; 75% O2: 25% CO2, 3 °C, 13 h led light exposition) for 5 d. Sensorial toughness was also evaluated at the end of the SRD. Ultrasonication slightly reduces 6–9% WHC of beef. HIU did not affect (p ˃ 0.05) water loss, meat colour, shear force and sensorial toughness of the meat. The Semimembranosus was the toughest muscle. Ultrasonication of 23-day-aged beef did not show improvements on quality characteristics, and despite minor changes in water loss and slight increase in shear force, consumers did not detect differences.
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Viter, Roman, Viktoriia Fedorenko, Inga Gabriunaite, Irina Tepliakova, Simonas Ramanavicius, Viktoriia Holubnycha, Arunas Ramanavicius i Aušra Valiūnienė. "Electrochemical and Optical Properties of Fluorine Doped Tin Oxide Modified by ZnO Nanorods and Polydopamine". Chemosensors 11, nr 2 (2.02.2023): 106. http://dx.doi.org/10.3390/chemosensors11020106.
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Various forms of zinc oxide (ZnO) are frequently used in the design of optical and electrochemical sensors. However, the optical and electrochemical properties of ZnO should be properly adjusted depending on the application area. Therefore, in this work, we have investigated changing/tuning the properties of ZnO by depositing a layer of polydopamine (PDA) on its surface. In order to perform this investigation, the surface of fluorine-doped tin oxide (FTO) was modified with the layer of ZnO nanorods and PDA. ZnO nanorods were synthesized by hydrothermal synthesis technique, and after the synthesis, they were coated with polydopamine exploiting the self-polymerization of dopamine. The nanostructures were investigated by using electrochemical and optical methods. Electrochemical impedance spectroscopy measurements showed that electrochemical properties of FTO-ZnO and FTO-ZnO-PDA nanostructures could be changed by the variation of both—applied electrical potential and/or exposition towards lighting. Interaction between ZnO-PDA and bovine serum albumin (BSA) molecules has been investigated by (photo)electrochemical and photoluminescence methods. A mechanism of possible interaction between BSA and the ZnO-PDA surface has been proposed.
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Tetschke, Florian, Jonas Golde, Tobias Rosenauer, Sabine Basche, Julia Walther, Lars Kirsten, Edmund Koch i Christian Hannig. "Correlation between Lesion Progression and Depolarization Assessed by Polarization-Sensitive Optical Coherence Tomography". Applied Sciences 10, nr 8 (24.04.2020): 2971. http://dx.doi.org/10.3390/app10082971.
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The detection of early stages of caries is still one of the major challenges in preservative dentistry. Since it is known from polarized light microscopy (PLM) that intrinsic enamel birefringence is affected by demineralization, polarization-sensitive optical coherence tomography (PSOCT) could facilitate the noninvasive detection and assessment of early carious lesions. The present study aims to correlate enamel lesion progression and depolarization measurements based on PSOCT in an artificial demineralization model. A total of 18 enamel slabs were prepared from bovine incisor teeth and demineralized in an acetic buffer solution for up to 49 days. The degree of polarization (DOP)—indicating depolarization and thus, demineralization—was calculated from PSOCT measurements and compared to lesion depth which was measured from PLM images. Artificial lesions showed characteristic zones of natural enamel demineralization in PLM images. DOP representations showed no depolarization for sound, nondemineralized enamel, whereas significant changes were found after 15 days of acid-exposition. The linear regression analysis of the DOP and the measured lesion depth showed a substantial correlation ( R 2 = 0.71 ). The results indicate that PSOCT-based depolarization imaging provides an unambiguous contrast for initial enamel demineralization which is correlated to the lesion progression.
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Hammad Aziz, Muhammad, Mahvish Fatima, Muhammad Waseem, Muhammad Fakhar-E-Alam, Muhammad Afzal i Muhammad Nadeem Shakoor. "Tumoricidal Effect of Hematite (α-Fe2O3) and SiO2 Nanoparticles in Human Rhabdomyosarcoma Cell Line". Advanced Materials Research 974 (czerwiec 2014): 235–40. http://dx.doi.org/10.4028/www.scientific.net/amr.974.235.
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Nanotechnology provides the opportunity for the development of new materials in the nanometer size range with many potential applications in biological sciences and clinical medicine. RD (muscle cancer cell line) was seeded out in 25 cm2plastic tissue culture flasks (NuncWiesbaden Germany) individually, in Minimum Essential Medium (MEM) with Hanks salts, containing 10% fetal bovine serum (FBS) and 2 mM L-Glutamine along with some nonessential amino acids and were incubated for 24 h for proper attachment to the substratum and kept at a 96 wells plate, incubated at 37°C and 5% CO2. SEM was employed to the nanoparticles and size of α-Fe2O3and SiO2nanoparticles were about 66 nm and 250 nm. Moreover 10-80μg/mL of Hematite (α-Fe2O3) and SiO2nanoparticles dispersed solution were labeled for each row of 96 wells plate. The present study evaluates the different parameters, e.g. time of incubation, cytotoxicity and cellular viability of the Human Rhabdomyosarcoma cell line (RD) as an experimental model. The viability of cells was determined by means of neutral red assay (NRA) after the cell-exposition to different concentrations of Hematite (α-Fe2O3) and SiO2nanoparticles into mentioned tumoricidal cells
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Arrigo, Federica, Francesca Arfuso, Federica Impellitteri, Claudia Giannetto, Giuseppe Piccione i Caterina Faggio. "Blood from Horses and Cows In Vitro Exposed to Quaternium-15 and Thiacloprid: Haematology and Erythrocyte Osmotic Fragility Alterations". Applied Sciences 13, nr 7 (30.03.2023): 4413. http://dx.doi.org/10.3390/app13074413.
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Blood cells are constantly exposed to several pollutants, including xenobiotics, and they can be considered a useful target for pollution exposition of the animal. The aim of this study was to investigate the effects of two xenobiotics (Quaternium-15, a preservative used in personal care products, and Thiacloprid, a neonicotinoid pesticide) on the haematological profile and erythrocyte osmotic fragility (EOF) of equine and bovine blood samples. Ten blood samples from horses and cows were exposed for 24 h to Quaternium-15 at a concentration of 0.1 and 1 mg/L and to Thiacloprid at a concentration of 30 and 60 µg/mL. A decrease in the values of the red blood cells, white blood cells, haematocrit, haemoglobin, mean corpuscular volume, and platelets, and an increase of EOF were found in blood samples exposed to xenobiotics compared to the control. According to the results gathered in the current study, the two xenobiotic compounds herein tested negatively affect the haematological indices causing haemolysis both in cattle and horse blood. This study, despite being preliminary, emphasizes the concept that blood cells are an excellent target for evaluating the effects of xenobiotics.
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Alves de Matos, A. P., P. Lehours, A. Timóteo, M. Roxo-Rosa i F. F. Vale. "Comparison of induction of B45 Helicobacter pylori prophage by acid and UV radiation". Microscopy and Microanalysis 19, S4 (sierpień 2013): 27–28. http://dx.doi.org/10.1017/s1431927613000755.
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Helicobacter pylori is a Gram-negative microorganism that grows on microaerophilic conditions and has only one known natural reservoir: the gastric mucosa. The infection by H. pylori is very common worldwide and this bacterium is associated with the development of gastritis, peptic ulcer gastric cancer or gastric Mucosa Associated Lymphoid Tissue (MALT) lymphoma. Although its natural habitat is the acidic gastric mucosa, H. pylori is considered to be a neutralophile. The bacterium survives brief exposure to pHs of <4, but growth occurs only at the relatively narrow pH range of 5.5 to 8.0, with optimal growth at neutral pH. Recently we have identified a prophage sequence (prophage phiHP33) in the strain B45, isolated from a patient diagnosed with gastric MALT lymphoma. This prophage revealed to be very difficult to induce. In fact, only few phage particles were observed on electron microscopy micrographs after exposure to UV radiation.In the present work we have compared the exposure to UV and to acidic environment in the induction of the prophage into a lytic cycle. We have tested two strains, the strain B45 carrying the prophage phiHP33 and a clinical strain 1152, isolated from a patient with peptic ulcer, that was revealed to be negative for the presence of integrase gene (a prophage gene essential for genome integration of prophage) by PCR, as negative control. Since the H. pylori reservoir is the human stomach the exposition to acid is very common, and with this experiment we intended to test if acid can trigger a phage lytic cycle.The induction using UV radiation has been previously described. For acid induction we have used a protocol adapted from Karita and Blaser. A 48 hours culture of H. pylori was grown in Brucella broth (Oxoid) supplemented with 10% of fetal bovine serum (Gibco) and 1% of Polivitex (BioMérieaux) in microaerophilic conditions at 37ºC. The liquid culture was centrifuged and the cell pellet ressuspended in citrate-phosphate buffer pH 6 and incubated 15 minutes, centrifuged again and ressuspended in citrate-phosphate buffer pH 3 and incubated for 30 minutes. After centrifugation the supernatant was recovered and incubated for 3 hours in phage precipitant (33% polyethylene glycol [PEG], 3M NaCl). After centrifugation at 10000 rpm for 10 minutes at 4ºC the pellet was ressuspended in phage buffer. These samples were analysed by transmission electron microscopy (TEM) after negative staining with 1% aqueous uranyl acetate, using a JEOL 100SX electron microscope.For B45 strain the induction using UV radiation (previously reported in Lehours, 2011) and acid exposure produced similar results (Figure 1 and Figure 2) showing numerous phage-like particles of about 100 nm diameter, apparently lacking a tail, after UV or acid exposition, respectively. These particles were not observed in the control strain 1152. Currently we are analysing the samples using molecular biology techniques and fixation embedding followed by ultrathin sectioning for TEM analysis, to detect the presence of phages.These preliminary results suggest that acid also appears to induce the H. pylori prophage phiHP33. However, since the number of phage particles observed is small, we can not rule out that the observed particles were released spontaneously. The exposition to the natural acidic environment of the human stomach may induce H. pylori prophage into a lytic cycle and to the propagation of phages among different H. pylori strains colonizing the same individual. Although highly speculative, transduction may be another form of horizontal gene transfer, which has not been described for this bacterium yet.Financial support received from the Portuguese Science and Technology foundation under the contract PTDC/EBB-EBI/119860/2010.
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Landeo-Villanueva, Guillermo Ernesto, María Elena Salazar-Salvatierra, Julio Reynaldo Ruiz-Quiroz, Noemi Zuta-Arriola, Benjamín Jarama-Soto, Oscar Herrera-Calderon, Josefa Bertha Pari-Olarte i Eddie Loyola-Gonzales. "Inhibitory Activity of Essential Oils of Mentha spicata and Eucalyptus globulus on Biofilms of Streptococcus mutans in an In Vitro Model". Antibiotics 12, nr 2 (10.02.2023): 369. http://dx.doi.org/10.3390/antibiotics12020369.
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The aim of this study was to evaluate the inhibitory activity of the commercially available essential oils of Mentha spicata (spearmint) and Eucalyptus globulus (eucalyptus) on Streptococcus mutans ATCC 25175 biofilms in vitro, emulating dental plaque conditions. The composition of the essential oils (EOs) was determined using gas chromatography coupled with mass spectrometry (GC-MS), with the main metabolites being Carvone (57.93%) and Limonene (12.91%) for Mentha spicata and 1,8-Cineole (Eucalyptol) (65.83%) for Eucalyptus globulus. The inhibitory activity was evaluated using the methods of agar-well diffusion and colorimetric microdilution. The inhibition halos were 18.3 ± 0.47 mm and 27.0 ± 0.82 mm, and the MICs were 1.8484 mg/mL and 1.9168 mg/mL for the EOs of Mentha spicata and Eucalyptus globulus, respectively. The activity against the biofilms was evaluated on a substrate of bovine enamel pieces using a basal mucin medium (BMM) in anaerobic conditions with daily sucrose exposition cycles in order to emulate oral cavity conditions. The EOs were applied in a concentration of 0.5% in a sterile saline vehicle with 1% polysorbate 20. After 72 h of cultivation, a significant reduction was observed (p < 0.001%) on the biofilm biomass, which was evaluated by its turbidity in suspension and using a count of the recoverable organisms with regards to the control. The effects of the Eos were not significantly distinct from each other. The EOs showed antimicrobial activity against both the Streptococcus mutans planktonic and biofilm cultures. Thus, EOs may have great potential for the development of pharmaceutical and sanitary products for oral health.
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Flemming, Jasmin, Clara Theres Meyer-Probst, Kristin Hille, Sabine Basche, Karl Speer, Isabelle Kölling-Speer, Christian Hannig i Matthias Hannig. "Olive Oil as a Transport Medium for Bioactive Molecules of Plants?—An In Situ Study". Molecules 28, nr 9 (28.04.2023): 3803. http://dx.doi.org/10.3390/molecules28093803.
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(1) Caries and erosions still remain a challenge for preventive dentistry. Certain plant extracts have shown beneficial effects in preventive dentistry. The aim of this study was to evaluate the antibacterial, anti-adherent and erosion-protective properties of ellagic acid (EA) as a polyphenolic agent. The combination with olive oil was investigated additionally to verify a possible improved bioactive effect of EA. (2) An in situ study was carried out with six subjects. Individual splints were prepared with bovine enamel specimens. The splints were worn for 1 min (pellicle formation time). Thereafter, 10 min rinses were performed with EA in water/in oil. Bacterial adherence was evaluated by fluorescence microscopy (DAPI, ConA, BacLight) after an 8 h oral exposition time. Additionally, the splints were worn for 30 min to quantify demineralization processes. The ultrastructure of the pellicle was investigated after an oral exposure time of 2 h under a transmission electron microscope. Statistical analysis was performed by Kruskal–Wallis tests, Mann–Whitney U tests and Bonferroni–Holm correction. (3) Rinsing with EA led to a significant reduction of adherent vital and dead bacteria. The combination with olive oil did not improve these outcomes. The assessment of glucan structures after rinsing with EA in water showed significant effects. Significant differences were observed for both rinses in calcium release at pH 3.0. After rinsing with EA in oil, significantly less calcium was released compared to rinsing with EA in water (pH = 3.0). (4) Olive oil is not suitable as a transport medium for lipophilic polyphenols. EA has anti-adherent and antibacterial properties in situ. EA also shows erosion-protective effects, which can be enhanced in combination with olive oil depending on the pH value. Ellagic acid has a neutral pH and could be an opportunity in the treatment of specific patient groups (xerostomia or mucositis).
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Saraiva, N. Z., C. S. Oliveira, T. A. D. Tetzner, M. R. de Lima, S. C. Méo i J. M. Garcia. "15 EFFECTS OF DEMECOLCINE ON THE MEIOTIC CELL-CYCLE AND MICROTUBULAR KINETICS OF ACTIVATED BOVINE OOCYTES SUBMITTED TO CHEMICAL ENUCLEATION". Reproduction, Fertility and Development 24, nr 1 (2012): 119. http://dx.doi.org/10.1071/rdv24n1ab15.
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Enucleation in traditional nuclear transfer (NT) is an invasive procedure for which alternative protocols have been sought. The present study was designed to explore the time course effects of demecolcine, a microtubule-depolymerizing agent, in bovine-activated oocytes submitted to induced chemical enucleation. For that purpose, after 26 h of in vitro maturation, the oocytes were parthenogenetically activated (5 μM ionomycin for 5 min and 10 μg mL–1 cycloheximide for 4 h) and treated with demecolcine (0.05 μg mL–1 for 2 h) 2 h after activation. Three groups were established: control (untreated oocytes), activated (oocytes exposed to activation) and deme (oocytes activated and treated with demecolcine). Then the nuclear and microtubular dynamics of the oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 59, 537–545). In each one of 3 replicates, 15 to 30 oocytes were evaluated per group. Oocytes were classified according to microtubule (MT) patterns as follows: present (evident MT), reduced (MT with reduced density), or absent. The results in percentage were submitted to ANOVA and means were compared by Tukey test, with a significance level of 5%. Effects of activation were observed after 2 h, when higher rates of oocytes presenting second polar body (2nd PB) extrusion were observed in the groups activated and deme (49.3% in both groups) compared with control (1.7%). At the end of activation treatment (4 h), the activated group presented 81.8% of oocytes with 2nd PB extrusion, whereas it was observed only in 37.8% of oocytes in the deme group. Effects of demecolcine on the microtubules initiated after only 0.5 h of treatment, when an increase (P < 0.05) of oocytes with reduced MT was observed in the deme group (26%; control – 3%; activated – 0%). After 6 h of culture in demecolcine-free medium, the deme group displayed ∼50% of oocytes with reduced MT (control – 0%; activated – 39%) and absence of MT in 23% of oocytes, which was superior to other groups (control – 0%; activated – 2%). Therefore, we detected a reduction of MT density after exposition of activated oocytes to demecolcine. However, MT were not completely absent in most of the evaluated oocytes, as previously described for bovine oocytes submitted to chemically assisted enucleation (Saraiva et al. 2009 Cloning Stem Cells 11, 141–152; Meng et al. 2011 Cell. Reprogram., in press). Apparently, there was no immediate repolymerization of MT after culture in demecolcine-free medium and this could result in negative consequences for subsequent embryo development. Moreover, demecolcine impaired the second PB extrusion during the activation process, probably due to inhibition of spindle rotation caused by the MT-disrupting drug. Nonetheless, considering the higher cytoplasmatic volume obtained with chemical enucleation and the lesser extent of injuries suffered by recipient oocytes, further studies focusing on the potential of embryo development and the quality of embryos are advisable. Financial support: FAPESP.
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Castangia, Ines, Federica Fulgheri, Matteo Perra, Gianluigi Bacchetta, Laura Fancello, Francesco Corrias, Iris Usach, Josè Esteban Peris, Maria Letizia Manca i Maria Manconi. "A Cocktail-Based Formula for the Design of Nanosized Cosmeceuticals as Skincare and Anti-Age Products". Nanomaterials 13, nr 17 (4.09.2023): 2485. http://dx.doi.org/10.3390/nano13172485.
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Nasco and Bovale grape pomace extracts, alone or in association, were loaded in nanoemulsions tailored for cosmetic application, using Kolliphor®RH40 (kolliphor) as the synthetic surfactant, Olivem®1000 (olivem) as the natural one, and lecithin as the cosurfactant. Pink transparent or milky dispersions, as a function of the used extract and surfactant, were obtained to be used as cosmeceutical serum or milk. The sizes of the nanoemulsion droplets were small (≈77 nm with kolliphor and ≈141 nm with olivem), homogenously dispersed (~0.24 with kolliphor and ~0.16 with olivem), highly negatively charged (≈−43 mV irrespective of the used surfactant) and their stability either on storage or under stressing conditions was affected by the used extract and surfactant. Formulations protected the extracts from the degradation caused by UV exposition, were biocompatible against keratinocytes, protected them against oxidative damages induced using hydrogen peroxide and inhibited the release of nitrite induced in macrophages using the lipopolysaccharide inflammatory stimulus. The overall results underlined the key role played by the composition of the formula to achieve a suitable cosmeceutical for skin care but even for the prevention of premature aging and chronic damages caused by the stressing conditions.
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Campos, Luana Vieira, Helena Roland Rogrigues Lima, Paula Cardoso Ferah, Luciana Tavares Perdigão i Neuza Rejane Wille Lima. "CONSTRUÇÃO COLABORATIVA DO CONHECIMENTO SOBRE O CONCEITO DE PLASTICIDADE FENOTÍPICA DO BOLDO BRASILEIRO QUANTO AO CRESCIMENTO E À REPRODUÇÃO". Revista Ciências & Ideias ISSN: 2176-1477 11, nr 1 (28.04.2020): 282. http://dx.doi.org/10.22407/2176-1477/2020.v11i1.1172.
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A plasticidade fenotípica é a capacidade de um genótipo expressar diferentes fenótipos em função do ambiente. O objetivo do presente estudo foi promover a coconstrução do conhecimento sobre a plasticidade fenotípica de plantas em uma turma de Engenharia Agrícola e Ambiental, aplicando duas aulas teórico-práticas com duas horas cada. Quarenta estacas apicais do Boldo-Brasileiro (Plectranthus barbatus Andrews) cresceram nas seguintes condições: i) cerca de 40 dias de cultivo; ii) sob 10%, 30%, 50% ou 100% de insolação; iii) em solos que diferem quanto ao tipo de adubo [(solo 1) húmus de minhoca + esterco bovino na proporção de 1:1 ou (solo 2) composto vegetal + esterco avícola na proporção de 1:3)]. Após aula expositiva de 30 minutos sobre plasticidade fenotípica em plantas, os 48 estudantes realizaram a biometria dos ramos principais do Boldo-Brasileiro para acessar comprimento, número de folhas, área foliar e número de brácteas nas inflorescências. Somente nas condições de maiores insolação o boldo expressou inflorescência. Ele expressou mais folhas quando cultivado no solo tipo 2 a 50% de insolação. O solo tipo 2 e a insolação de 10% favoreceu o crescimento em altura. As distâncias entre nós foram menores para os maiores graus de insolação e solo tipo 1. A área foliar foi maior para os menores graus de insolação e solo tipo 2. As fotos das plantas realizadas pelos estudantes, as comparações que estes fizeram para os resultados biométricos, as redações realizadas em grupo e seus relatos obtidos durante a roda de conversa revelaram que estes coconstruíram seus conhecimentos sobre estratégia fisiológica das plantas frente às variáveis ambientais. As interações entre professor, mediadora e estudantes estabelecidas durante as duas aulas teórico-práticas atingiram o que havia sido proposto. Assim, todo processo serviu como metodologia que pode auxiliar na desburocratização do ensino de Ciências.
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Toishibekov, Y. M., i H. D. Blackburn. "120 SUCCESSFUL LAMBING AFTER SUPER-COOLING ULTRARAPID VITRIFICATION SHEEP MORULAE". Reproduction, Fertility and Development 22, nr 1 (2010): 218. http://dx.doi.org/10.1071/rdv22n1ab120.
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The aim of this work was to establish alternative methods for sheep morulae cryopreservation by using vitrification by open pulled straw (OPS) methods and super-cooling ultra-rapid vitrification (SCURV). Both treatments used a vitrification solution (VS) of 20% (3.6 mol L-1) ethylene glycol (EG), 20% (2.4 mol L-1) dimethylsulfoxide (DMSO), 0.5 mol L-1 sucrose in DPBS with 10% BSA in both methods. In our experiment we used the Vit-Master™ (MTG, Germany). The super-cooled LN facilitates heat transmission between LN and the cryosolution interface, and this is efficient for bovine semen and blastocyst cryoconservation (Arav et al. 2002). By surgical flushing of 24 super stimulated ewes 121 transferrable morulae were harvested; 30 morulae were transferred fresh to synchronised recipients and the others were cryopreserved by OPS (n = 49) or SCURV (n = 42), respectively thawed or warmed, and transferred to recipients. Embryos were vitrified using the OPS method. They were first incubated in 50% VS for 2 min and then transferred for 30 s into 100% VS. Each embryo was loaded by touching a 1-μL drop with the straw, which was immediately submerged into and stored in liquid nitrogen. Warming was done by placing the narrow end of the straw into DPBS + 0.25M sucrose for 5 min. Embryos were then transferred into DPBS + 0.125 M sucrose for 3 min and finally to DPBS until transfer. The SCURV morulae were then exposed to 50 and 100% VS at 37°C for 2 min and 30 s, respectively. Embryos after saturation VS have been transferred by on a surface of a nylon loop (volume 20 μL, diameter 0.5 mm) and using negative pressure temperature of liquid nitrogen in the chamber for freezing with the VIT-Master. Thawing vitrified embryos was accomplished by placing the vitrified embryos in solutions of sucrose 0.25 and 0.125 with expositions of 2 and 3 min, accordingly. After embryos were thawed, only good quality embryos were transferred. Importantly, our data suggest that by using the SCURV method, the toxic elements contained in the cryopreservation solution can be reduced while maintaining a similar ability to produce viable morulae for implantation as the OPS method. Although further work on the developmental competence of embryos cryopreserved with the SCURV method are needed, these data suggest that the faster freeze rate and lower levels of cryoprotectants of SCURV are able to minimize ice crystal formation and should be further evaluated as a routine mechanism for cryopreserving sheep morulae. Table 1.Effect vitrification and ultra-rapid super-cooling vitrification on the viability and lambing of sheep morulae
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Valieva, G. A., M. M. Toishibekov, S. M. Askarov i B. B. Molzhigitov. "57 THE EFFECT OF VITRIFICATION FOR SHEEP EMBRYO VIABILITY". Reproduction, Fertility and Development 27, nr 1 (2015): 121. http://dx.doi.org/10.1071/rdv27n1ab57.
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This work evaluated different methods for sheep embryo cryopreservation by vitrification (V) and super-cooling ultra-rapid vitrification (SCURV). The vitrification method was applied according to the method described by Vajta et al. Both treatments used a vitrification solution (VS) containing 20% ethylene glycol, 20% dimethylsulfoxide (Me2SO), 0.5 mol L–1 sucrose in Dulbecco's phosphate buffered saline (DPBS) with 10% BSA. The super-cooled LN facilitates heat transmission between LN and the cryosolution interface, and this is efficient for bovine semen and blastocyst cryoconservation (Arav et al. 2002). By surgical flushing 25 super-stimulated ewes, 109 transferable morulae were harvested; 35 morulae were transferred fresh to synchronized recipients (control) and the others were cryopreserved by V (n = 36) or SCURV (n = 38), respectively, thawed or warmed, and transferred to recipients. Embryos were vitrified using the HSV Kit. They were first incubated in 50% VS for 2 min and then transferred for 30 s into 100% VS. Each embryo was loaded by HSV Kit, which was immediately submerged into and stored in LN. Warming was done by placing the narrow end of the straw into DPBS + 0.25 M sucrose for 5 min. Embryos were then transferred into DPBS + 0.125 M sucrose for 3 min and finally to DPBS until transfer. The SCURV morulae were then exposed to 50 and 100% VS at 37°C for 2 min and 30 s, respectively. Embryos after saturation in VS were transferred on a surface of a nylon loop (volume 20 μL, diameter 0.5 mm) and using negative pressure of LN in the chamber for freezing with the VIT-Master. Thawing vitrified embryos was accomplished by placing the vitrified embryos in solutions of sucrose 0.25 M and 0.125 M with expositions 2 and 3 min accordingly. After thawing embryos, only good-quality embryos were transferred. Statistical analyses were performed with Student's t-test. The lambing rate following transfer of fresh, frozen-thawed vitrification and SCURV methods were 18, 12, 14 lambs accordingly. No statistical difference was found for the percentage of does lambing following transfer thawed after vitrification (33.4 ± 5.2a%) and SCURV methods (36.8 ± 6.3b%). The survival rate following transfer of fresh embryos (51.4 ± 4.8c) was higher and in line with previous findings using VS. Differences were statistically significant (ac,bc P < 0.05). Importantly, our data suggest that the HSV Kit can be used to produce viable morulae for implantation as the SCURV, and to as vitrification method. Although further work on the developmental competence of embryos cryopreserved with the SCURV method are needed, these data suggest that with SCURV a faster freeze rate and lower level of cryoprotectants is able to minimize ice crystal formation and should be further evaluated as a routine mechanism for cryopreserving sheep embryos.
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Ogbanya, K. C., i C. A. Eze. "Gross and radiographic assessment of foot with claw disorders in culled cattle at Nsukka abattoir". Nigerian Journal of Animal Production 47, nr 5 (31.12.2020): 58–65. http://dx.doi.org/10.51791/njap.v47i5.1326.
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Claw disorders are either infectious or non-infectious which have been found to be the cause of more than 90% of all lameness cases observed in dairy cattle. Against this background, descriptive and cross-sectional study was to determine the common radiographic features of claws with gross disorders using abattoir samples. A total of 40 claws were collected from Nsukka abattoir. The claws were examined for identification of different types of gross lesions. Two radiographic exposures, dorso-plantar and lateral views of each of the claws were taken and evaluated for radiographic changes. White line degeneration (WLD), sole erosion and horn heel erosion were the most prevalent gross lesions with 35%, 30% and 20% prevalent rates respectively. Bulb erosion and claw wall fissure were the least occurred claw disorders with 5% and 5% occurrence. Infectious claw disorders (55%) had a relatively higher occurrence than the non-infectious disorders (45%). The most prominent radiographic abnormalities were noted in the second (PII) and third phalanxes (PIII) region. The radiographic changes were sinking of PIII (10%) subluxation of PII (5%), foreign body penetration (15%), exostoses (5%) of the pastern (PII) bone. Laminitis occurred in 30% of the sampled claws. Concurrent appearance of more than one radiographic feature in a single claw was noted in the claw samples. In conclusion, radiographic examination revealed that typical changes of chronic laminitis were among the most common pathological features recorded. Les troubles des griffes sont soit infectieux, soit non infectieux, ce qui s'est avéré être la cause de plus de 90% de tous les cas de boiterie observés chez les bovins laitiers. Dans ce contexte, une étude descriptive et transversale visait à déterminer les caractéristiques radiographiques communes des griffes présentant des troubles macroscopiques à l'aide d'échantillons d'abattoir. Au total, 40 griffes ont été collectées à l'abattoir de Nsukka. Les griffes ont été examinées pour identifier différents types de lésions macroscopiques. Deux expositions radiographiques, vues dorso-plantaire et latérale de chacune des griffes ont été prises et évaluées pour les changements radiographiques. La dégénérescence de la ligne blanche (le 'WLD'), l'érosion de la sole et l'érosion du talon de la corne étaient les lesions macroscopiques les plus fréquentes avec respectivement 35%, 30% et 20% de taux de prévalence. L'érosion des bulbes et la fissure de la paroi des griffes ont été les moins affectées des griffes avec 5% et 5% d'occurrence. Les troubles infectieux des griffes (55%) avaient une fréquence relativement plus élevée que les troubles non infectieux (45%). Les anomalies radiographiques les plus importantes ont été notées dans la région de la deuxième (le 'PII') et de la troisième phalange (le 'PIII'). Les changements radiographiques étaient la descente de la subluxation 'PIII' (10%) du 'PII' (5%), la pénétration de corps étrangers (15%), les exostoses (5%) du paturon (le 'PII'). Une laminite est survenue dans 30% des griffes échantillonnées. L'apparition simultanée de plus d'une caractéristique radiographique dans une seule griffe a été notée dans les échantillons de griffe. En conclusion, l'examen radiographique a révélé que les changements typiques de la fourbure chronique faisaient partie des caractéristiques pathologiques les plus courantes enregistrées.
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Derceli, Juliana dos Reis, Juliana Jendiroba Faraoni, Pâmella Coelho Dias, Laís Lopes Machado de Matos i Regina Guenka Palma-Dibb. "Effect of gaseous hydrochloric acid on human and bovine enamel". Clinical and Laboratorial Research in Dentistry, 16.11.2020. http://dx.doi.org/10.11606/issn.2357-8041.clrd.2020.171222.
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Objective: To evaluate the action of gaseous hydrochloric acid on human and bovine enamel and compare the demineralization pattern of these substrates exposed to the gaseous erosive agent. Methods: Eight bovine enamel and eight human enamel specimens were obtained (4 × 4 × 2 mm), half surface was protected with composite resin and the other half was exposed to gaseous hydrochloric acid (gHCl), pH 2 at 37 °C, for 3 min, 8 times a day, for 12 days, and in intervals the specimens were maintained in artificial saliva. The specimens were analyzed according to wear profile, surface roughness and microhardness (before and after acid exposition). Data were statistically analyzed by one-way ANOVA. Results: Data showed no significant difference between bovine and human enamel for all properties analyzed – microhardness (98.1 ± 5.2, 96.9 ± 4.8), wear profile (11.5 ± 2.8, 11.4 ± 3.6) and roughness (2.6 ± 0.3, 3.3 ± 0.3), respectively. In images, we observed that gHCl could cause enamel erosion in both groups. Conclusion: Gaseous hydrochloric acid causes similar enamel erosion on bovine and humans.
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SIMONNEAUX, Laurence. "Une démarche pour élaborer des panneaux de préfiguration d'une exposition sur les biotechnologies de la reproduction bovine". Didaskalia, nr 11 (1997). http://dx.doi.org/10.4267/2042/23846.
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Kirsch, Jasmin, Matthias Hannig, Pia Winkel, Sabine Basche, Birgit Leis, Norbert Pütz, Anna Kensche i Christian Hannig. "Influence of pure fluorides and stannous ions on the initial bacterial colonization in situ". Scientific Reports 9, nr 1 (grudzień 2019). http://dx.doi.org/10.1038/s41598-019-55083-0.
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AbstractThe present clinical-experimental study aims to examine the effect of pure experimental fluoride solutions and stannous chloride on the initial oral bioadhesion under in situ conditions. After 1 min of pellicle formation on bovine enamel slabs, 12 subjects rinsed with 8 ml of the fluoride test solutions (NaF, Na2PO3F, AmF, SnF2,) with 500 ppm fluoride concentration each for 1 min. Additionally, rinsing without a solution (control) and rinsing with 1563 ppm SnCl2 solution took place for 1 min. Afterwards, fluorescence microscopy took place to visualize bacterial adhesion and glucan formation (8 h oral exposition) with DAPI and ConA and the BacLight method. TEM was performed to visualize the pellicle ultrastructure together with EDX to detect stannous ions. The rinsing solutions with pure SnF2 and SnCl2 reduced significantly the initial bacterial colonization (DAPI). While, NaF and Na2PO3F showed no significant effect compared to the control. There was no significant difference between AmF, SnF2 and SnCl2. All tested experimental solutions showed no reducing effect on the glucan formation. Considerable alterations of the pellicle ultrastructure resulted from rinsing with the Sn-containing solutions. SnF2 appears to be the most effective type of fluoride to reduce initial bacterial colonization in situ. The observed effects primarily have to be attributed to the stannous ions’ content.
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Dolcini, Jacopo, Elisa Ponzio, Marcello Mario D’Errico i Pamela Barbadoro. "Socioeconomic differences in dietary habits in Italy before and during COVID-19 pandemic: secondary analysis of a nationwide cross-sectional study". BMC Public Health 24, nr 1 (11.01.2024). http://dx.doi.org/10.1186/s12889-023-17530-6.
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Abstract Background Several socioeconomic conditions may influence subjects’ adherence to healthy eating habits. Food consumption may be influenced by external stress during crisis periods; however, the effects of these events on food habits are difficult to predict. Also, a pandemic crisis like the recent COVID-19 pandemic may have influenced dietary habits and food consumption. The objective of this study was to compare the dietary habits of Italian people before the COVID-19 pandemic with those belonging to the year 2020 in a nationwide population sample. Materials and methods Information on dietary habits has been obtained from the multi-purpose survey on families’ “Aspects of daily life”, carried out in Italy by the Italian National Statistics Institute (ISTAT). We analyzed data coming from 2016 and 2020 editions of the survey (43,000 subjects each year). We used population attributable fraction (PAF) adjusted for age, defined as the proportional reduction in unhealthy diet that would occur if all participants had had a higher education, assuming higher educated individuals as more socially advantaged. Prevalence association for each dietary exposition has been calculated through logistic regression. Results Looking at aggregated data from 2016 and 2020 both men and women showed a high prevalence of unhealthy dietary habits. Regarding men, excessive consumption of eggs, pork meat, and bovine meat was characterized by a PAF attributable to socioeconomic conditions to an extent greater than 30%. Women showed the same trend. Focusing on different years of investigation, in 2020, during the COVID-19 pandemic, men and women increased their consumption of eggs, cooked fats, snacks, and sweets, and reduced consumption of fruits and vegetables. Additionally, women increased the assumption of dietary products and meat. Both sexes registered an increase in overweight and obese subjects in 2020. Conclusions To our knowledge, this study was the first in our country to use a yearly, nationwide sample to analyze dietary habits by examining specific types of various foods on a nationwide scale and establishing a correlation between these habits and the COVID-19 pandemic. Our results showed unbalanced dietary habits of the Italian population with an excess of consumption of several foods like eggs, cooked fats snacks, and sweets with low consumption of fruits and vegetables. Socioeconomic differences influence food choices but in a complex way since they seemed to affect some wrong dietary habits but not others, especially regarding fruits and vegetables assumption where differences were less evident among social classes. Outside stressors like a crisis period such as the COVID-19 pandemic seem to have an important role in both men and women regarding the assumption of so-called “junk food”.
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Bulla-Castañeda, Diana María, Deisy Johana Lancheros-Buitrago, Diego Jose García-Corredor, Julio C. Giraldo-Forero i Martin Orlando Pulido-Medellin. "Seroprevalence and risk factors of bovine fasciolosis in the municipalities of Colombia". Veterinary World, czerwiec 2023, 1293–300. http://dx.doi.org/10.14202/vetworld.2023.1293-1300.
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Background and Aim: Bovine fasciolosis is a reemerging neglected disease with a worldwide distribution caused by the trematode Fasciola spp., which parasitize various hosts. Bovine fasciolosis is responsible for large economic losses in the bovine livestock sector. This study aimed to estimate the seroprevalence and risk factors of bovine fasciolosis in the municipalities of Colombia. Materials and Methods: This was a descriptive cross-sectional study with simple random sampling conducted on 1140 cattle from the municipalities of Chiquinquir´, San Miguel de Sema, and Ubaté for a duration of 3 months. Serum samples were processed using the commercial Fasciola hepatica Antibody Test Kit IDEXX® Fasciolosis Verification (IDEXX, United States), which identified immunoglobulin G antibodies for gf2 antigen purified from Fasciola extracts. The f2 antigen is extremely immunogenic and highly specific for F. hepatica. An epidemiological survey was performed to record variables related to the sampled animals and herd management practices. Data were processed using the statistical program Epi Info® (Centers for Disease Control and Prevention; Atlanta, Georgia). The prevalence ratio was estimated to evaluate the association between fasciolosis and the hypothesized causal factors and the significance of this association using Pearson’s Chi-square test. Finally, a logistic regression model was developed. Results: The overall seroprevalence was 72.3%. The seroprevalence was 83.9% (323/385) in Chiquinquirá, 68.17% (257/377) in Ubaté, and 64.55% (244/378) in San Miguel de Sema. The seroprevalence was higher in male animals in Chiquinquirá and in female animals in San Miguel de Sema and Ubaté. Similarly, sex showed a statistically significant association with disease prevalence in Ubaté. The highest prevalence was found in cattle aged >2 years. The Holstein breed showed maximum seroprevalence in Chiquinquirá (p ≤ 0.05) and San Miguel de Sema, whereas crossbreed showed higher seroprevalence in Ubaté. Similarly, in Chiquinquirá, the association between the seroprevalence of fasciolosis and the presence of other species was statistically significant (95% confidence interval [CI]: 0.9601–3.4944; p = 0.0448). In Ubaté, the disease presentation was also associated with pasture rental (95% CI: 0.4047–1.0023; p = 0.003) and attendance to livestock expositions (95% CI: 0.2313–1.0636; p = 0.044). However, in San Miguel de Sema, water from the stream showed a statistically significant association with disease presentation (95% CI: 0.5209–1.0985; p = 0.00649785). Female sex and diarrhea occurrence were considered risk factors for fasciolosis. Conclusion: A high seroprevalence of antibodies to Fasciola spp. was detected in cattle in the study municipalities, indicating a high parasite distribution in these areas. Female sex and diarrhea were established as risk factors associated with fasciolosis in Ubaté and San Miguel de Sema, respectively. Further, research is necessary to establish prevention and control programs against parasitosis. Keywords: bovine, fasciolosis, risk factors, seroprevalence.