Gotowe bibliografie tematyczne / ELISA

Gotowa bibliografia na temat „ELISA”

Autor: Grafiati
Data publikacji: 4 czerwca 2021
Data aktualizacji: 6 lipca 2024

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Artykuły w czasopismach na temat "ELISA"

1

Veling, J., F. G. van Zijderveld, A. M. van Zijderveld-van Bemmel, H. W. Barkema i Y. H. Schukken. "Evaluation of Three Newly Developed Enzyme-Linked Immunosorbent Assays and Two Agglutination Tests for Detecting Salmonella enterica subsp. enterica Serovar Dublin Infections in Dairy Cattle". Journal of Clinical Microbiology 38, nr 12 (2000): 4402–7. http://dx.doi.org/10.1128/jcm.38.12.4402-4407.2000.

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In this study test characteristics of three newly developed enzyme-linked immunosorbent assays (ELISAs) for Salmonella enterica subsp. enterica serovar Dublin were evaluated and compared with two agglutination tests. The ELISAs involved were an indirect ELISA with serovar Dublin lipopolysaccharide (LPS ELISA), an indirect ELISA with serovar Dublin flagellar antigen (GP ELISA), and a double-antibody sandwich blocking ELISA that uses monoclonal antibodies against S. enterica subsp.enterica serovar Enteritidis flagellin (GM-DAS ELISA). The agglutination tests involved were two routine serum agglutination tests with either somatic (O) or flagellar (H) antigen. Diagnostic specificity of the three ELISAs was determined using 840 serum samples from seven dairy herds without any history of serovar Dublin infection. Cutoff values at a titer of 100, 100, and 10, respectively, for the LPS ELISA, GP ELISA, and GM-DAS blocking ELISA resulted in a specificity of 99.3, 100, and 100%, respectively. Using these cutoff values the LPS ELISA, GP ELISA, and GM-DAS ELISA were able to detect, respectively, 30, 46, and 38% of 50 fecal culture-positive animals from 13 herds with a recent serovar Dublin infection. With the same cutoff values, active carriers (n = 18) were detected for 94.4% with the LPS ELISA and for 100% with the GP and GM-DAS ELISAs. Kappa values determined on the results of all tests from 8 of the 13 serovar Dublin-infected herds and the 7 control herds demonstrated a good correlation between the results of all ELISAs and the H-agglutination test. The results of the O-agglutination test failed to correlate with those of the other tests. Using a set of sera from 170 aborting cows (with 25 abortions due to serovar Dublin), test results of the ELISAs and the H-agglutination test were comparable. The H-agglutination test may be used successfully for single sample testing, especially to diagnose abortion due to serovar Dublin. It is concluded that the ELISAs are useful diagnostic tools in serovar Dublin control programs and that they are preferred to agglutination tests for reasons of automation and costs.
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Tenter, A. M., C. Vietmeyer, A. M. Johnson, K. Janitschke, M. Rommel i W. Lehmacher. "ELISAs based on recombinant antigens for sero-epidemiological studies onToxoplasma gondiiinfections in cats". Parasitology 109, nr 1 (lipiec 1994): 29–36. http://dx.doi.org/10.1017/s0031182000077738.

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SUMMARYTwo recombinantToxoplasma gondiipolypeptides, H4 and H11, were tested as diagnostic antigens in enzyme-linked immunosorbent assays (ELISAs). The results obtained by ELISAs based on single H4 (H4-ELISA), on single H11 (H11-ELISA) and on a mixture of H4 and H11 (H4/H11-ELISA) were compared with results obtained by an ELISA based on traditional ELISA antigen (TEA-ELISA), an indirect fluorescent antibody test (IFAT), the Sabin-Feldman dye test (SFDT) and a direct agglutination test (DAT). A total of 306 cats from a suburban cat population were tested of which about 45% showed serological evidence ofT. gondiiinfection. Infection rates varied from about 32% for cats kept indoors to about 55% for stray cats. Specificities > 99% were observed for all ELISAs based on the recombinant antigens (H4-ELISA, H11-ELISA and H4/H11-ELISA). The H4/H11-ELISA also reached a sensitivity of 95% which compared very favourably with those observed for the TEA-ELISA (98%) and for the IFAT (94%). Negative and positive predictive values for the H4/H11-ELISA were 96 and < 99%, respectively. Antibody titres measured by the H4/H11-ELISA also correlated well with those measured by the SFDT and the DAT. Hence, the H4/H11-ELISA appears to be a very suitable test for sero-epidemiological studies onT. gondiiinfections in cats.
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McKenna, S. L. B., D. C. Sockett, G. P. Keefe, J. McClure, J. A. VanLeeuwen i H. W. Barkema. "Comparison of Two Enzyme-Linked Immunosorbent Assays for Diagnosis of Mycobacterium Avium Subsp. Paratuberculosis". Journal of Veterinary Diagnostic Investigation 17, nr 5 (wrzesień 2005): 463–66. http://dx.doi.org/10.1177/104063870501700510.

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Enzyme-linked immunosorbent assays (ELISAs) are used in Johne's disease (JD) control programs as a first screening for presence of the disease in a herd. A high sensitivity of the ELISA is therefore important, yet the commonly used ELISAs have relatively low sensitivity. The inclusion of an absorption phase, although improving specificity, potentially decreases sensitivity. Sera and feces of 383 adult dairy cows in 8 herds were used to compare the test characteristics of an absorbed and a nonabsorbed indirect ELISA for the detection of JD. The absorbed ELISA is based on a protoplasmic antigen, whereas the nonabsorbed uses a lipoarabinomannan-based antigen. The potential advantage of the nonabsorbed ELISA is that it may be less specific and more sensitive. Two herds certified free of JD were used to compare the specificity of the ELISAs. The other herds used to compare sensitivity were either infected with Mycobacterium avium subsp. paratuberculosis or had unknown status. Using fecal culture as a gold standard, the diagnostic specificity for the absorbed and nonabsorbed ELISAs were 98.4% and 87.9%, respectively. The diagnostic sensitivity was 72.4% and 65.5% for the absorbed and the nonabsorbed ELISA, respectively. Furthermore, a comparison using a fecal DNA probe as the comparison standard resulted in both ELISAs having a sensitivity of 61.9%. Agreement between the 2 ELISAs was moderate, with a kappa statistic of 0.58. The nonabsorbed ELISA did not have a higher sensitivity and had a lower specificity than the absorbed ELISA. Therefore, in this population, there was no advantage gained with using the nonabsorbed ELISA.
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Kande, Sylvie. "Elisa". Callaloo 20, nr 3 (1997): 646–50. http://dx.doi.org/10.1353/cal.1998.0076.

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Fereig, Ragab M., Sarah A. Altwaim i Caroline F. Frey. "Evaluation of a Commercial Serum Competitive Enzyme-Linked Immunosorbent Assay for Detection of Neospora caninum-Specific Antibodies in Raw Milk of Ruminants". Parasitologia 4, nr 2 (27.03.2024): 91–98. http://dx.doi.org/10.3390/parasitologia4020008.

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Bovine neosporosis is an infection caused by the protozoan parasite Neospora caninum and has substantial veterinary hazards. Neosporosis cannot be controlled by vaccination or chemotherapy. Thus, accurate diagnosis followed by isolation and culling of infected animals is regarded as the most efficient method of control. In vivo diagnosis often relies on serologic testing of the animals, and milk represents a non-invasive and easy-to-collect sample matrix. However, indirect enzyme-linked immunosorbent assay (ELISA) specifically designed for antibody detection in milk are sometimes not easily available and it is tempting to use ELISA kits that are originally designed for use in serum in milk samples instead. Herein, we evaluated a widely used commercial ELISA (ID Screen® Neospora caninum competition Multispecies ELISA (ID. Vet, Grabels, France)), developed for detection of N. caninum antibodies in serum samples, for its performance on milk samples. Milk samples from dairy ruminants (cows, buffaloes, sheep, and goats; n = 149) were tested in parallel with the serum ELISA and a commercial milk ELISA as a standard test (Neospora caninum Milk Competitive ELISA, ID. Vet, Grabels, France). The detected prevalence values were 28.2% (42/149), 17.4% (26/149), and 17.4% (26/149) using milk ELISA, serum ELISA, and both ELISAs, respectively. Sensitivity, specificity, positive predictive value, and negative predictive value for the serum ELISA used with milk samples were 61.9%, 100%, 100%, and 87%, respectively. The agreement and kappa value between the two ELISAs were 89.3% and 0.70, respectively, suggesting substantial agreement. High values of Pearson correlation coefficient (0.904, p ≥ 0.0001) and area under the receiver operating characteristic (ROC) curve (0.789, p ≥ 0.0001) demonstrated the high diagnostic performance of the serum ELISA in milk samples. Also, a Bland–Altman Plot and histogram describing the frequency of distribution of ELISA optical densities confirmed the high agreement of both serum and milk ELISAs. The current results revealed the high specificity but moderate sensitivity of the serum ELISA used for milk samples compared with the milk ELISA. However, the excellent positive predictive value of the serum ELISA makes it an alternative option in case of the unavailability of milk ELISAs. With this study, we provided additional evidence that a widely used serum ELISA test kit may also be used for the detection of N. caninum antibodies in milk samples.
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6

Nielsen, Flemming A. J. "Litterær shamanisme i Kongebøgerne – sagaen om Elias og Elisa". Dansk Teologisk Tidsskrift 78, nr 3 (10.10.2015): 185–201. http://dx.doi.org/10.7146/dtt.v78i3.105812.

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The Saga of Elijah and Elisha (1 Kgs 16:29 – 2 Kgs 13:25) deals with the history of the cult of Ba’al in Biblical Israel. Its nucleus is a mosaic of 35 episodes containing several versions of the biographies of Elijah and Elisha who are atypical Old Testament prophets belonging to a select circle of “men of God”. Their saga supplements and comments on the greater story of Biblical Israel, and it is argued that there is an affinity between them and the shamans known in particular from the Arctic world and the aboriginal peoples of the Americas. Definitions of prophets and shamans are briefly discussed, and the kind of shamanism associated with Elijah and Elisha is described and named literary shamanism.
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Morenkov, O. S., Nadja Fodor i I. Fodor. "Indirect Elisas Based on Recombinant and Affinity-Purified Glycoprotein E of Aujeszky's Disease Virus to Differentiate Between Vaccinated and Infected Animals". Acta Veterinaria Hungarica 47, nr 1 (1.01.1999): 137–50. http://dx.doi.org/10.1556/avet.47.1999.1.15.

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Two indirect ELISAs for the detection of antibodies against glycoprotein E (gE) of Aujeszky's disease virus (ADV) in sera have been developed. The rec-gE-ELISA is based on theE. coli-expressed recombinant protein containing the N-terminal sequences of gE (aa 1-125) fused with the glutathione S-transferase fromSchistosoma japonicum. The affi-gE-ELISA is based on native gE, which was purified from virions by affinity chromatography. The tests were optimised and compared with each other, as well as with the recently developed blocking gE-ELISA (Morenkov et al., 1997b), with respect to specificity and sensitivity. The rec-gE-ELISA was less sensitive in detecting ADV-infected animals than the affi-gE-ELISA (sensitivity 80% and 97%, respectively), which is probably due to the lack of conformation-dependent immunodominant epitopes on the recombinant protein expressed inE. coli. The specificity of the rec-gE-ELISA and affi-gE-ELISA was rather moderate (90% and 94%, respectively) because it was necessary to set such cut-off values in the tests that provided a maximum level of sensitivity, which obviously increased the incidence of false positive reactions. Though the indirect ELISAs detect antibodies against many epitopes of gE, the blocking gE-ELISA, which detects antibodies against only one immunodominant epitope of gE, showed a better test performance (specificity 99% and sensitivity 98%). This is most probably due to rather high dilutions of the sera used in the indirect gE-ELISAs (1:30) as compared to the serum dilution in the blocking gE-ELISA (1:2). We conclude that the indirect gE-ELISAs are sufficiently specific and sensitive to distinguish ADV-infected swine from those vaccinated with gE-negative vaccine and can be useful, in particularly affi-gE-ELISA, as additional tests for the detection of antibodies to gE.
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Veling, J., F. G. van Zijderveld, A. M. van Zijderveld-van Bemmel, Y. H. Schukken i H. W. Barkema. "Evaluation of Two Enzyme-Linked Immunosorbent Assays for Detecting Salmonella enterica subsp.enterica Serovar Dublin Antibodies in Bulk Milk". Clinical Diagnostic Laboratory Immunology 8, nr 6 (1.11.2001): 1049–55. http://dx.doi.org/10.1128/cdli.8.6.1049-1055.2001.

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ABSTRACT Two enzyme-linked immunosorbent assays (ELISAs) for the detectingSalmonella enterica subsp. entericaserovar Dublin antibodies in bulk milk were developed and evaluated for potential use in control programs. The ELISAs were based on either lipopolysacharide (LPS ELISA) or flagellar antigen (GP ELISA). Sensitivity was determined with 79 case herds with a wide range of clinical signs. Specificity was determined with 125 Dutch and 200 Swedish control herds. The relation between antibodies in bulk milk, antibodies in serum, and the level of milk production of individual cows was studied with 61 case herds. The optimal optical density (OD) values of the LPS ELISA and the GP ELISA were determined to be 0.2 and 0.5, respectively. The sensitivities of the LPS ELISA and the GP ELISA were 54 and 63%, respectively, with a specificity of 98% for both ELISAs with samples from the Dutch control herds. The specificities for samples from the Swedish herds were 100% for the LPS ELISA and 95% for the GP ELISA. The sensitivity of the combination of tests was 65% when samples were run in parallel, and the specificity was 100% when samples were run in series, irrespective of whether the samples came from Dutch or Swedish control herds. The variance (R 2) in the OD value for bulk milk samples could be explained by the percentage of seropositive lactating cows in a herd with the LPS ELISA for 51% of the samples and with the GP ELISA for 72%. The variance in the OD value was best explained by the combination of the percentage of seropositive lactating cows in the herd and the mean log10 serum antibody titer for that herd (R 2 = 62% for the LPS ELISA andR 2 = 75% for the GP ELISA). Case herds more often tested negative by the ELISA with bulk milk when the percentage of seropositive lactating cows was less than 5%. It is concluded that both ELISAs with bulk milk can be used in control programs to distinguish between infected and noninfected herds. Specificity can be increased by using the two tests in combination. Sensitivity was relatively low for both single tests and both tests combined.
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Prieto, José M., Ana Balseiro, Rosa Casais, Naiara Abendaño, Liam E. Fitzgerald, Joseba M. Garrido, Ramon A. Juste i Marta Alonso-Hearn. "Sensitive and Specific Enzyme-Linked Immunosorbent Assay for Detecting Serum Antibodies against Mycobacterium avium subsp. paratuberculosis in Fallow Deer". Clinical and Vaccine Immunology 21, nr 8 (28.05.2014): 1077–85. http://dx.doi.org/10.1128/cvi.00159-14.

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ABSTRACTThe enzyme-linked immunosorbent assay (ELISA) is the diagnostic test most commonly used in efforts to control paratuberculosis in domestic ruminants. However, commercial ELISAs have not been validated for detecting antibodies againstMycobacterium aviumsubsp.paratuberculosisin wild animals. In this study, we compared the sensitivities and specificities of five ELISAs using individual serum samples collected from 41 fallow deer with or without histopathological lesions consistent with paratuberculosis. Two target antigenic preparations were selected, an ethanol-treated protoplasmic preparation obtained from a fallow deerM. aviumsubsp.paratuberculosisisolate (ELISAs A and B) and a paratuberculosis protoplasmic antigen (PPA3) (ELISAs C and D). Fallow deer antibodies bound to the immobilized antigens were detected by using a horseradish peroxidase (HRP)-conjugated anti-fallow deer IgG antibody (ELISAs A and C) or HRP-conjugated protein G (ELISAs B and D). A commercially available assay, ELISA-E, which was designed to detectM. aviumsubsp.paratuberculosisantibodies in cattle, sheep, and goats, was also tested. Although ELISAs A, C, and E had the same sensitivity (72%), ELISAs A and C were more specific (100%) for detecting fallow deer with lesions consistent with paratuberculosis at necropsy than was the ELISA-E (87.5%). In addition, the ELISA-A was particularly sensitive for detecting fallow deer in the latent stages of infection (62.5%). The antibody responses detected with the ELISA-A correlated with both the severity of enteric lesions and the presence of acid-fast bacteria in gut tissue samples. In summary, our study shows that the ELISA-A can be a cost-effective diagnostic tool for preventing the spread of paratuberculosis among fallow deer populations.
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van Hoeven, Karen H., Connie Dale, Phil Foster i Barbara Body. "Comparison of Three Enzyme-Linked Immunosorbent Assays for Detection of Immunoglobulin G Antibodies to Tetanus Toxoid with Reference Standards and the Impact on Clinical Practice". Clinical and Vaccine Immunology 15, nr 12 (8.10.2008): 1751–54. http://dx.doi.org/10.1128/cvi.00254-08.

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ABSTRACT Accurate determination of the concentrations of immunoglobulin G (IgG) antibody to tetanus toxoid is important in order to evaluate the immunogenicity of tetanus toxoid vaccines, determine immune competence in individual patients, and measure the prevalence of immunity in populations. The performance of three commercially available enzyme-linked immunosorbent assays (ELISAs) for IgG antibodies to tetanus toxoid were evaluated. Serially diluted NIBSC 76/589 and TE-3 human tetanus IgG immunoglobulin international reference standards were analyzed in quadruplicate using ELISAs manufactured by The Binding Site, Inc. (VaccZyme); Scimedx; and Euroimmun. In addition, IgG antibodies to tetanus toxoid were measured in 83 deidentified serum specimens using each manufacturer's ELISA. Each ELISA provided linear results when evaluated with the reference preparations. The Binding Site ELISA provided results that closely corresponded to the reference preparations (y = 1.09x − 0.08), whereas the Scimedx ELISA gave results that were consistently lower (y = 0.21x − 0.07) and the Euroimmun ELISA gave results that were consistently higher (y = 1.5x + 0.30) than the reference preparation concentrations. Using the recommended cutoff for each ELISA (<0.10 IU/ml), the overall agreement of all of the ELISA methods was 78%. Three of eighty-three (3.6%) human serum samples demonstrated inadequate immunity with all three assays. The Binding Site ELISA yielded nonprotective antibody concentrations in only these 3 samples, whereas 19 samples (22.9%) according to the Scimedx ELISA and 6 samples (7.2%) according to the Euroimmun ELISA demonstrated nonprotective concentrations. The performance characteristics of ELISAs for tetanus immunoglobulin titers were manufacturer dependent, and the differences translated into important disparities in reported results.
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Rozprawy doktorskie na temat "ELISA"

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Kornemann, Elisa [Verfasser]. "Entwicklung einer Röntgenzoomlinse / Elisa Kornemann". Karlsruhe : KIT Scientific Publishing, 2019. http://www.ksp.kit.edu.

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Degner, Elisa [Verfasser]. "Vorstandsinnenhaftung nach Kartellrechtsverstößen / Elisa Degner". Baden-Baden : Nomos Verlagsgesellschaft mbH & Co. KG, 2021. http://d-nb.info/123310926X/34.

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Pohl, Johanna Sophie [Verfasser]. "iFGF-23-ELISA versus cFGF-23-ELISA als kardiovaskuläre Prädiktoren bei chronischer Nierenerkrankung / Johanna Sophie Pohl". Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2020. http://d-nb.info/1235399591/34.

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Brüning, Anke. "Serodiagnosis of equine babesiosis by ELISA". Thesis, Imperial College London, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.306891.

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Johnson, Maureen Jane. "Coproantigen capture ELISA for GI nematodes". Thesis, University of Nottingham, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.240993.

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MASSON, JEFERSON ALVES. "ELISA LISPECTOR: RECORDS OF A MEETING". PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2015. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=25567@1.

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PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO
COORDENAÇÃO DE APERFEIÇOAMENTO DO PESSOAL DE ENSINO SUPERIOR
PROGRAMA DE SUPORTE À PÓS-GRADUAÇÃO DE INSTS. DE ENSINO
Esta pesquisa partiu das leituras da obra de Elisa Lispector e da seguinte indagação do pesquisador: por que Elisa, publicada, considerada pela crítica e premiada na segunda metade do século XX no Brasil, caiu no esquecimento? A proposta é resgatar os motivos de interesse do trabalho da escritora. Dentre tais motivos, destacam-se -- em construção autônoma, mas inter-relacionada -- a busca da memória de uma família judaica expatriada, que fugiu da Ucrânia para o Brasil, e o questionamento constante da identidade e das relações interpessoais no contexto da dimensão temporal. As personagens, quer evidenciem ou não traços (auto)biográficos, delineiam-se, ao longo dos contos e romances, sempre empenhadas na construção de sua subjetividade. A tarefa projetada desenvolveu-se com o apoio das entrevistas feitas pelo autor da dissertação e de sua coleção de críticas referentes aos livros de Elisa, desenhos e pinturas do seu acervo, bem como fotos da família Lispector. O enfoque da situação da escritora e de sua obra teve, como principal referência teórica, os princípios da crítica biográfica, orientando não apenas a leitura de registros da vida de Elisa em contraponto à análise de amostras escolhidas de seus escritos, como também a inserção das experiências do pesquisador para a construção da figura da escritora. O objetivo maior é evidenciar a importância do resgate da obra de Elisa Lispector para a literatura brasileira.
This research started from readings of Elisa Lispector and the question: why has her work, published, well regarded by critics and awarded prizes in the second half of the twentieth century in Brazil, fallen into neglect? The idea is to highlight the points of interest in Lispector s work. Foremost among these are the following (each a good reason in its own right, but all interconnected): the memories of an expatriate Jewish family who fled Ukraine for Brazil, and the constant questioning of identity and interpersonal relations over time. The characters—whether or not they contain (auto) biographical features—in her short stories and novels are always involved with the construction of their own subjectivity. This study relied on the interviews made by the author and on his collection of criticism related to Elisa Lispector s books, drawings and paintings belonging to her, as well as photographs of the Lispector family. The basic theoretical underpinnings of this study of a writer s life and work are the principles of biographical criticism. They provide guidelines not only for the reading of the records of a writer s life in the light of analyses of samples chosen from her writings, but also for an understanding of the researcher s own experiences in his construction of the figure of Elisa Lispector. The major goal here is to underscore the importance of rediscovering her work for Brazilian literature.
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Medeiros, Carla Leite. "Vacina de DNA utilizando genes sintéticos derivados do peptídeo SBm7462 contra o carrapato Rhipicephalus (Boophilus) microplus e avaliação da resposta imune em camundongos Balb/c". Universidade Federal de Viçosa, 2008. http://locus.ufv.br/handle/123456789/4956.

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Made available in DSpace on 2015-03-26T13:46:35Z (GMT). No. of bitstreams: 1 texto completo.pdf: 729175 bytes, checksum: d8be6fdfabfabf889bd81cc7f09142f9 (MD5) Previous issue date: 2008-05-16
The Rhipicephalus (Boophilus) microplus is one of the most important arthropods in veterinary medicine due economic losses and health problems caused in cattle production, mainly in Central and South America, as well as in Australia. As an alternative to chemical control, immunization of bovines with Bm86 antigen to induce a protective immune response. A synthetic peptide, SBm 7462, derived from Bm86, has been shown great results in control of ticks. The construction and synthesis of one nucleotide sequence based on this peptide might be useful for design a DNA vaccine that has many advances than peptide vaccine. A gene, called seq1, was constructed with a three repetition of nucleotide sequence of SBm 7462. It was cloned into a pCIneo vector expression in mammals, transfected in VERO cells and injected in BALB/c mouse. Two methods were used to analysis of peptide expression in vitro: DOT ELISA and PAP. In both, the results showed that the nucleotide sequence (seq1) had not been express in VERO cells. In vivo, when mice were inoculated with the expression cassette they did not response in ELISA. They elevated antibody titles only when vaccinated with the syntetic peptide SBm 7462. And, the best titles of immunoglobulins were seen when the SBm 7462 was administered subcutaneously. After that, we insert a mutation at the begging of seq1, but, the sequenciament demonstrated that any initiation codon (ATG) had been inserted.
O Rhipicephalus (Boophilus) microplus é um dos mais importantes artrópodes em medicina veterinária devido perdas econômicas e problemas de saúde causados na produção de gado na América Central e do Sul, bem como na Austrália. Como alternativa ao controle químico, a imunização de bovinos com antígeno proteíco Bm86 induz uma resposta imune protetora. Um peptídeo sintético, SBm 7462, derivado da Bm86, tem obtido excelentes resultados no controle de carrapatos. A construção e síntese de uma sequência nucleotidíca baseada neste peptídeo podem ser útil para o desenho de uma vacina de DNA que tem muitas vantagens sob uma vacina sintética. Um gene, denominado seq1, foi construído repetindo três vezes a sequência nucleotidídica do SBm 7462. Ele foi clonado no vetor de expressão em mamíferos, pCIneo, transfectado em células VERO e injetado em camundongos BALB/c. Dois métodos foram usados para análise da expressão do peptídeo in vitro: DOT ELISA e PAP. Em ambos, os resultados demonstraram que a seqüência nucleotídica (seq1) não havia sido expressa em células VERO. In vivo, quando camundongos foram inoculados com o cassete de expressão eles não responderam ao ELISA. Eles elevaram os títulos de anticorpos, apenas, quando inoculados com o peptídeo sintético SBm 7462. Os melhores títulos de imunoglobulinas foram vistos quando o SBm 7462 foi administrado subcutâneamente. Após isso, inserimos uma mutação no início do seq 1, porém, o sequenciamento demonstrou que nenhum códon de iniciação (ATG) tinha sido inserido.
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Wolf, Elisa Maria [Verfasser]. "Druckkündigungen mit diskriminierendem Hintergrund / Elisa Maria Wolf". Frankfurt : Peter Lang GmbH, Internationaler Verlag der Wissenschaften, 2012. http://d-nb.info/104242232X/34.

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Gruber, Elisa [Verfasser], i Werner [Gutachter] Lang. "Vaskuläre Malformationen / Elisa Gruber ; Gutachter: Werner Lang". Erlangen : Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 2017. http://d-nb.info/1140917102/34.

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Hoven, Elisa [Verfasser]. "Rechtsstaatliche Anforderungen an völkerstrafrechtliche Verfahren. / Elisa Hoven". Berlin : Duncker & Humblot, 2012. http://d-nb.info/1238427928/34.

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Książki na temat "ELISA"

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Crowther, John R. ELISA. New Jersey: Humana Press, 1995. http://dx.doi.org/10.1385/0896032795.

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Ferrero, Ernesto. Elisa. Palermo: Sellerio, 2002.

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Hnasko, Robert, red. ELISA. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2742-5.

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Matson, Robert S., red. ELISA. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2903-1.

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Molins, Manuel. Elisa. Tarragona: Arola Editors, 2003.

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Delvalle, Alcibíades González. Elisa. Asunción, Paraguay: CID, 1986.

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Molins, Manuel. Elisa. Tarragona [Spain]: Arola Editors, 2003.

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Elisa. Cognac: Le Temps qu'il fait, 2003.

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Elisa. Ospedaletto, Pisa: Pacini, 2009.

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Elisa: Roman. [Montréal, Québec]: Stanké, 1995.

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Części książek na temat "ELISA"

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Brewer, William Dean, i Alfredo Tiomno Tolmasquim. "Elisa". W Jayme Tiomno, 93–109. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-41011-7_7.

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Schwab, Manfred. "ELISA". W Encyclopedia of Cancer, 1. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-642-27841-9_1849-2.

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Sataloff, Johnathan, i Robert T. Sataloff. "ELISA". W Encyclopedia of Otolaryngology, Head and Neck Surgery, 743. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-23499-6_200189.

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Lasseter, Benjamin F. "Elisa". W Biochemistry in the Lab, 31–35. Names: Lasseter, Benjamin F., author. Title: Biochemistry in the lab : a manual for undergraduates / by Benjamin F. Lasseter. Description: Boca Raton, Florida : CRC Press, [2020]: CRC Press, 2019. http://dx.doi.org/10.1201/9780429491269-4.

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Meyer, Torsten. "Competitive ELISA". W Antibody Engineering, 739–42. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-01144-3_47.

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Schewe, Christiane, Wilko Weichert i Manfred Dietel. "PCR-ELISA". W Guidelines for Molecular Analysis in Archive Tissues, 117–20. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-17890-0_24.

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Lin, Alice V. "Indirect ELISA". W Methods in Molecular Biology, 51–59. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2742-5_5.

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Lin, Alice V. "Direct ELISA". W Methods in Molecular Biology, 61–67. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2742-5_6.

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Jain, Aakanchha, Richa Jain i Sourabh Jain. "ELISA Reader". W Basic Techniques in Biochemistry, Microbiology and Molecular Biology, 33–34. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-4939-9861-6_15.

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Makarananda, Kittima, Lucinda R. Weir i Gordon E. Neal. "Competitive ELISA". W Immunochemical Protocols, 155–60. Totowa, NJ: Humana Press, 1998. http://dx.doi.org/10.1007/978-1-59259-257-9_15.

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Streszczenia konferencji na temat "ELISA"

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Fitzsimons, E. D., N. Brandt, U. Johann, S. Kemble, H. R. Schulte, D. Weise i T. Ziegler. "Elisa technology consolidation study overview". W International Conference on Space Optics 2014, redaktorzy Bruno Cugny, Zoran Sodnik i Nikos Karafolas. SPIE, 2017. http://dx.doi.org/10.1117/12.2304251.

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Ogrič, M., P. Žigon, K. Lakota, S. Praprotnik, D. Drobne, B. Štabuc, S. Sodin-Semrl i S. Čučnik. "SAT0192 Competitive elisa and bridging elisa with acid dissociation detect anti-drug antibodies in a greater proportion of patients treated with tnf-Αlpha inhibitors than classical bridging elisa". W Annual European Congress of Rheumatology, EULAR 2018, Amsterdam, 13–16 June 2018. BMJ Publishing Group Ltd and European League Against Rheumatism, 2018. http://dx.doi.org/10.1136/annrheumdis-2018-eular.6486.

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Tan, Xiaotian, Qiushu Chen, Hongbo Zhu, Yuan Gong, Yu-Cheng Chen, Xuzhou Li, Xiaoqin Wu, Maxwell W. Li, Wenyi Liu i Xudong Fan. "A fast and reproducible ELISA laser platform". W Frontiers in Biological Detection: From Nanosensors to Systems XI, redaktorzy Benjamin L. Miller, Sharon M. Weiss i Amos Danielli. SPIE, 2019. http://dx.doi.org/10.1117/12.2507517.

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YANG, XIAOLIN. "LUMINESCENT PCR-ELISA ASSAY TO DETECT TB". W Proceedings of the 11th International Symposium. WORLD SCIENTIFIC, 2001. http://dx.doi.org/10.1142/9789812811158_0090.

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Kohn, Anna, Maudi de Jong, Merlijn van den Berg, Arjan Kwakernaak, Ester van Leeuwen, Arianne Brandsma i Dieneke Schonenberg-Meinema. "P12 Sensitivity differences in ELISA versus EliA for detecting anti-dsDNA antibodies: awareness for the clinician". W 14th European Lupus Meeting, Bruges, Belgium, March 19–22, 2024. Lupus Foundation of America, 2024. http://dx.doi.org/10.1136/lupus-2024-el.66.

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Bloom, J. W., J. A. Berkner i G. Mitra. "FACTOR VIII:C, 80 KD AND 90-210 KD POLYPEPTIDE QUANTITATION BY SLISA". W XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644042.

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Purified Factor VIII:c is generally found proteolyzed into nultiple polypeptides. An 80 kD polypeptide appears to form a metal-linked complex with each of a series of 90-210 kD polypeptides and separation of these complexes results in loss af Factor VIII:c activity. To quantitate total Factor VIII:c content - active and inactive - three sandwich ELISA's were developed using an immunopurified polyclonal antibody as the capture antibody. This antibody was shown by a Western blot of Factor VIII:c to interact with all the 80-210 kD polypeptides. Utilizing biotinylated polyclonal antibody, an ELISA was developed that detected both the purified carboxy terminal (80 kD) and amino terminal (90-210 kD) polypeptides. Thrombin treated 80 kD polypeptide and Factor VIII:c were also detected by this ELISA. A second ELISA was developed with a biotinylated monoclonal, designated C8, that detected purified 90-210 kD polypeptides, but not the 80 kD polypeptide. A third ELISA was developed with a biotinylated monoclonal, designated C7F7, that detected only the purified 80 kD functional subunit of Factor VIII:c. Thrombin treated 80 kD polypeptide and Factor VIII:c were not detected by this ELISA. These three ELISA methods combined allow estimates of amounts of denatured or thrombin degraded Factor VIII:c to be determined, information not obtainable by the coagulation activity assays.
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Ingerslev, J., S. Stenbjerg, A. Bukh, NPH Møller i J. Zeuthen. "EVIDENCE FOR AN ABNORMAL EXPRESSION OF THE COLLAGEN BINDING DOMAIN IN VON WILLEBRAND'S DISEASE TYPE II". W XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644644.

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A recently developed new series of monoclonal antibodies (MAbs) against the von Willebrand factor (vWf) included antibodies strongly inhibiting ( Mab vWf-41) and partly inhibiting ( Mab vWf-33) the collagen binding of vWf. We also characterized two Mabs with interacting properties against the ristocetin induced platelet aggregation (MAbs vWf-21 and vWf-39). These antibodies were conjugated with horse-radish peroxidase (HRP) and examined in different constructions forming two-site MAb ELISA's for plasma vWf:Ag and compared with polyclonal antibody ELISA. Symmetrical MAb-ELISA ( i.e. same Mab for extraction and detection) gave practical no dose-response in the standard assay, whereas any different combination of Mabs gave favourable dose-response relationships in sensitive ELISA's for vWf:Ag. Two different sandwiches were chosen using MAb vWf-33 and Mab vWf-41 at either side of the ELISA. These two assay models gave results of plasma from normal persons almost identical to those obtained with polyclonal antibody ELISA. Also in type I von Willebrand's disease these three assays performed very uniformly. In subtypes II plasma ( IIA: n=7; IIB: n=3, IIC: n=l, IID: n=i) . the assay using vWf-33 for coating and vWf-41-HRP for detection measured considerably lower than the polyclonal ELISA and the Mab-ELISA based on the opposite combination. We believe, that our results are indicative of a molecular defect in the collagen binding domain of vWf in subtype II plasma.
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Liu, X. Y., C. M. Cheng, A. W. Martinez, K. A. Mirica, X. J. Li, S. T. Phillips, M. Mascarenas i G. M. Whitesides. "A portable microfluidic paper-based device for ELISA". W 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2011. http://dx.doi.org/10.1109/memsys.2011.5734365.

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Ogirala, Tejaswi, Ashley Eapen, Katrina G. Salvante, Pablo A. Nepomnaschy i M. Ash Parameswaran. "Adaptive illumination backlight panel for ELISA imaging systems". W 2016 IEEE Canadian Conference on Electrical and Computer Engineering (CCECE). IEEE, 2016. http://dx.doi.org/10.1109/ccece.2016.7726838.

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Váradi, K., J. Kárpáti i S. Elödi. "ENZYME LINKED IMMUNOASSAY (ELISA) FOR FACTOR VIII ANTIGEN". W XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644033.

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A two site ELISA test was developed for measuring factor VIII antigen (FVIIIsAg). The assay is based on two antibodies developed in a non-haemophilic and in a severe haemophilia-A patient, respectively. The IgG fraction prepared from the non-haemophilic plasma was used for coating, and the IgG isolated from the haemophilia-A plasma was labelled with horse-radish peroxidaseFVIII:Ag and FVIII activity was measured in 28 healthy blood donors and in 41 haemophilia-A patients. The normal range for FVIIIcAg was 40 - 180 %, the correlation coefficient between FVIII:Ag and FVIII activity assays was 0.8. The sensitivity of the assay ranges between 0.005 - 0.2 U/ml FVIII:Ag. In 18 cases of severe haemophilia-A FVIII:Ag was not detectable. In 3 out of 23 mild haemophilia-A cases FVIIIrAg was significantly higher, then FVIII activity, indicating CRM variants of the disease. Due to the high sensitivity of FVIII:Ag detection, the assay appears to be suitable for prenatal diagnosis
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Raporty organizacyjne na temat "ELISA"

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Perk, Simon, Egbert Mundt, Alexander Panshin, Irit Davidson, Irina Shkoda, Ameera AlTori i Maricarmen Garcia. Characterization and Control Strategies of Low Pathogenic Avian Influenza Virus H9N2. United States Department of Agriculture, listopad 2012. http://dx.doi.org/10.32747/2012.7697117.bard.

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The avian influenza virus, subtype H9N2 subtype, defined as having a low pathogenicity, causes extensive economical losses in commercial flocks, probably due to management and synergism with other pathogens. AIV H9N2 was first identified in Israel in the year 2000, and since then it became endemic and widespread in Israel. Control by vaccination of commercial flocks with an inactivated vaccine has been introduced since 2007. In face of the continuous H9N2 outbreaks, and the application of the vaccination policy, we aimed in the present study to provide a method of differentiating naturally infected from vaccinated animals (DIVA). The aim of the assay would be detect only antibodies created by a de-novo infection, since the inactivated vaccine virus is not reproducing, and might provide a simple tool for mass detection of novel infections of commercial flocks. To fulfill the overall aim, the project was designed to include four operational objectives: 1. Evaluation of the genetic evolution of AIV in Israel; 2. Assessment of the diagnostic value of an NS1 ELISA; 3. NS1 ELISA as evaluation criteria for measuring the efficacy of vaccination against H9N2 AIV; 4. Development of an AIV H9 subtype specific ELISA systems. Major conclusion and implications drawn from the project were: 1. A continuous genetic change occurred in the collection of H9N2 isolates, and new introductions were identified. It was shown thatthe differences between the HA proteins of viruses used for vaccine productionand local fieldisolatesincreasedin parallelwith the durationand intensity ofvaccine use, therefore, developing a differential assay for the vaccine and the wild type viruses was the project main aim. 2. To assess the diagnostic value of an NS1 ELISA we first performed experimental infection trials using representative viruses of all introductions, and used the sera and recombinant NS1 antigens of the same viruses in homologous and heterologous NS1 ELISA combination. The NS1 ELISA was evidently reactive in all combinations, and did not discriminate significantly between different groups. 3. However, several major drawbacks of the NS1 ELISA were recognized: a) The evaluation of the vaccination effect in challenged birds, showed that the level of the NS1 antibodies dropped due to the vaccination-dependent virus level drop; b) the applicability of the NS1-ELISA was verified on sera of commercial flocks and found to be unusable due to physico-chemical composition of the sera and the recombinant antigen, c) commercial sera showed non-reactivity that might be caused by many factors, including vaccination, uncertainty regarding the infection time, and possibly low antigen avidity, d) NS1 elevated antibody levels for less than 2 months in SPF chicks. Due to the above mentioned reasons we do not recommend the application of the DIVA NS1 ELISA assay for monitoring and differentiation AIV H9N2 naturally-infected from vaccinated commercial birds.
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Magtoto, Ronaldo L., John K. Johnson i Sheela Ramamoorthy. Lawsonia intracellularis ELISA: A New Test at the ISU-VDL. Ames (Iowa): Iowa State University, styczeń 2010. http://dx.doi.org/10.31274/ans_air-180814-645.

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Shope, Robert E., Ben-Ami Peleg i Jerry S. Walker. Detection of Rift Valley Fever ELISA Antibody and Antigen in Livestock. United States Department of Agriculture, czerwiec 1985. http://dx.doi.org/10.32747/1985.7598183.bard.

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Henderson, C. C., i A. K. Singh. LDRD final report on microencapsulated immunoreagents for development of one-step ELISA. Office of Scientific and Technical Information (OSTI), sierpień 1997. http://dx.doi.org/10.2172/554806.

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Durant, J. T. Evaluation of ELISA screening test for detecting aflatoxin in biogenic dust samples. Office of Scientific and Technical Information (OSTI), maj 1996. http://dx.doi.org/10.2172/573251.

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Little, S. F., W. M. Webster, S. L. Norris i G. P. Andrews. Evaluation of an Anti-rPA IgG ELISA for Measuring the Antibody Response in Mice. Fort Belvoir, VA: Defense Technical Information Center, styczeń 2004. http://dx.doi.org/10.21236/ada428618.

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Kaattari, Stephen L. ELISA-Based Segregation of Adult Spring Chinook Salmon for Control of Bacterial Kidney Disease: Annual Report 1991. Office of Scientific and Technical Information (OSTI), luty 1993. http://dx.doi.org/10.2172/920640.

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Minchev, Velko, Kalina Kamenova, Nadya Hristova-Avakumova, Slavina Surcheva i Rumen Nikolov. ihydropyrimidine Dehydrogenase (DPD) Screening in Blood Plasma of Cancer Patients Indicated to Fluoropyrimidine Chemotherapy by ELISA Method. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, maj 2021. http://dx.doi.org/10.7546/crabs.2021.05.15.

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Kaattari, Stephen L., i James R. Winton. ELISA-Based Segregation of Adult Spring Chinook Salmon for Control of Bacterial Kidney Disease, Annual Report FY 1989. Office of Scientific and Technical Information (OSTI), grudzień 1989. http://dx.doi.org/10.2172/920196.

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Winton, James R., i Stephen L. Kaattari. ELISA-Based Segregation of Adult Spring Chinook Salmon for Control of Bacterial Kidney Disease, Annual Report FY 1990. Office of Scientific and Technical Information (OSTI), grudzień 1990. http://dx.doi.org/10.2172/5208467.

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