Gotowe bibliografie tematyczne / Electrophoresis microchip devices

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Gotowa bibliografia na temat „Electrophoresis microchip devices”

Autor: Grafiati
Data publikacji: 6 września 2023

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Artykuły w czasopismach na temat "Electrophoresis microchip devices"

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Munro, Nicole J., Karen Snow, Jeffrey A. Kant i James P. Landers. "Molecular Diagnostics on Microfabricated Electrophoretic Devices: From Slab Gel- to Capillary- to Microchip-based Assays for T- and B-Cell Lymphoproliferative Disorders". Clinical Chemistry 45, nr 11 (1.11.1999): 1906–17. http://dx.doi.org/10.1093/clinchem/45.11.1906.

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Abstract Background: Current methods for molecular-based diagnosis of disease rely heavily on modern molecular biology techniques for interrogating the genome for aberrant DNA sequences. These techniques typically include amplification of the target DNA sequences followed by separation of the amplified fragments by slab gel electrophoresis. As a result of the labor-intensive, time-consuming nature of slab gel electrophoresis, alternative electrophoretic formats have been developed in the form of capillary electrophoresis and, more recently, multichannel microchip electrophoresis. Methods: Capillary electrophoresis was explored as an alternative to slab gel electrophoresis for the analysis of PCR-amplified products indicative of T- and B-cell malignancies as a means of defining the elements for silica microchip-based diagnosis. Capillary-based separations were replicated on electrophoretic microchips. Results: The microchip-based electrophoretic separation effectively resolved PCR-amplified fragments from the variable region of the T-cell receptor-γ gene (150–250 bp range) and the immunoglobulin heavy chain gene (80–140 bp range), yielding diagnostically relevant information regarding the presence of clonal DNA populations. Although hydroxyethylcellulose provided adequate separation power, the need for a coated microchannel for effective resolution necessitated additional preparative steps. In addition, preliminary data are shown indicating that polyvinylpyrrolidone may provide an adequate matrix without the need for microchannel coating. Conclusions: Separation of B- and T-cell gene rearrangement PCR products on microchips provides diagnostic information in dramatically reduced time (160 s vs 2.5 h) with no loss of diagnostic capacity when compared with current methodologies. As illustrated, this technology and methodology holds great potential for extrapolation to the abundance of similar molecular biology-based techniques.
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Ha, Ji Won. "Acupuncture Injection Combined with Electrokinetic Injection for Polydimethylsiloxane Microfluidic Devices". Journal of Analytical Methods in Chemistry 2017 (2017): 1–6. http://dx.doi.org/10.1155/2017/7495348.

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We recently reported acupuncture sample injection that leads to reproducible injection of nL-scale sample segments into a polydimethylsiloxane (PDMS) microchannel for microchip capillary electrophoresis. The advantages of the acupuncture injection in microchip capillary electrophoresis include capability of minimizing sample loss and voltage control hardware and capability of introducing sample plugs into any desired position of a microchannel. However, the challenge in the previous study was to achieve reproducible, pL-scale sample injections into PDMS microchannels. In the present study, we introduce an acupuncture injection technique combined with electrokinetic injection (AICEI) technique to inject pL-scale sample segments for microchip capillary electrophoresis. We carried out the capillary zone electrophoresis (CZE) separation of FITC and fluorescein, and the mixture of 10 μM FITC and 10 μM fluorescein was separated completely by using the AICEI method.
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Fister, Julius C., Stephen C. Jacobson i J. Michael Ramsey. "Ultrasensitive Cross-Correlation Electrophoresis on Microchip Devices". Analytical Chemistry 71, nr 20 (październik 1999): 4460–64. http://dx.doi.org/10.1021/ac990853d.

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Chen, Yu-Hung, Wei-Chang Wang, Kung-Chia Young, Ting-Tsung Chang i Shu-Hui Chen. "Plastic Microchip Electrophoresis for Analysis of PCR Products of Hepatitis C Virus". Clinical Chemistry 45, nr 11 (1.11.1999): 1938–43. http://dx.doi.org/10.1093/clinchem/45.11.1938.

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Abstract Background: Electrophoresis on polymeric rather than glass microstructures is a promising separation method for analytical chemistry. Assays on such devices need to be explored to allow assessment of their utility for the clinical laboratory. Methods: We compared capillary and plastic microchip electrophoresis for clinical post-PCR analysis of hepatitis C virus (HCV). For capillary electrophoresis (CE), we used a separation medium composed of 10 g/L hydroxypropyl methyl cellulose in Tris-borate-EDTA buffer and 10 μmol/L intercalating dye. For microchip electrophoresis, the HCV assay established on the fused silica tubing was transferred to the untreated polymethylmethacrylate microchip with minimum modifications. Results: CE resolved the 145-bp amplicon of HCV in 15 min. The confidence interval of the migration time was <3.2%. The same HCV amplicon was resolved by microchip electrophoresis in <1.5 min with the confidence interval of the migration time <1.3%. Conclusion: The polymer microchip, with advantages that include fast processing time, simple operation, and disposable use, holds great potential for clinical analysis.
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Rodríguez, Isabel, Lian Ji Jin i Sam F. Y. Li. "High-speed chiral separations on microchip electrophoresis devices". Electrophoresis 21, nr 1 (1.01.2000): 211–19. http://dx.doi.org/10.1002/(sici)1522-2683(20000101)21:1<211::aid-elps211>3.0.co;2-d.

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Kumar, Suresh, Vishal Sahore, Chad I. Rogers i Adam T. Woolley. "Development of an integrated microfluidic solid-phase extraction and electrophoresis device". Analyst 141, nr 5 (2016): 1660–68. http://dx.doi.org/10.1039/c5an02352a.

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Vrouwe, Elwin X., Regina Luttge, Istvan Vermes i Albert van den Berg. "Microchip Capillary Electrophoresis for Point-of-Care Analysis of Lithium". Clinical Chemistry 53, nr 1 (1.01.2007): 117–23. http://dx.doi.org/10.1373/clinchem.2007.073726.

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Abstract Background: Microchip capillary electrophoresis (CE) is a promising method for chemical analysis of complex samples such as whole blood. We evaluated the method for point-of-care testing of lithium. Methods: Chemical separation was performed on standard glass microchip CE devices with a conductivity detector as described in previous work. Here we demonstrate a new sample-to-chip interface. Initially, we took a glass capillary as a sample collector for whole blood from a finger stick. In addition, we designed a novel disposable sample collector and tested it against the clinical standard at the hospital (Medisch Spectrum Twente). Both types of collectors require &lt;10 μL of test fluid. The collectors contain an integrated filter membrane, which prevents the transfer of blood cells into the microchip. The combination of such a sample collector with microchip CE allows point-of-care measurements without the need for off-chip sample treatment. This new on-chip protocol was verified against routine lithium testing of 5 patients in the hospital. Results: Sodium, lithium, magnesium, and calcium were separated in &lt;20 s. The detection limit for lithium was 0.15 mmol/L. Conclusions: The new microchip CE system provides a convenient and rapid method for point-of-care testing of electrolytes in serum and whole blood.
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Ludwig, Martin, i Detlev Belder. "Coated microfluidic devices for improved chiral separations in microchip electrophoresis". ELECTROPHORESIS 24, nr 15 (sierpień 2003): 2481–86. http://dx.doi.org/10.1002/elps.200305498.

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Kricka, Larry J. "Miniaturization of analytical systems". Clinical Chemistry 44, nr 9 (1.09.1998): 2008–14. http://dx.doi.org/10.1093/clinchem/44.9.2008.

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Abstract Miniaturization has been a long-term trend in clinical diagnostics instrumentation. Now a range of new technologies, including micromachining and molecular self-assembly, are providing the means for further size reduction of analyzers to devices with micro- to nanometer dimensions and submicroliter volumes. Many analytical techniques (e.g., mass spectrometry and electrophoresis) have been successfully implemented on microchips made from silicon, glass, or plastic. The new impetus for miniaturization stems from the perceived benefits of faster, easier, less costly, and more convenient analyses and by the needs of the pharmaceutical industry for microscale, massively parallel drug discovery assays. Perfecting a user-friendly interface between a human and a microchip and determining the realistic lower limit for sample volume are key issues in the future implementation of these devices. Resolution of these issues will be important for the long-term success of microminiature analyzers; in the meantime, the scope, diversity, and rate of progress in the development of these devices promises products in the near future.
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Gibson, Larry R., i Paul W. Bohn. "Non-aqueous microchip electrophoresis for characterization of lipid biomarkers". Interface Focus 3, nr 3 (6.06.2013): 20120096. http://dx.doi.org/10.1098/rsfs.2012.0096.

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In vivo measurements of lipid biomarkers are hampered by their low solubility in aqueous solution, which limits the choices for molecular separations. Here, we introduce non-aqueous microchip electrophoretic separations of lipid mixtures performed in three-dimensional hybrid nanofluidic/microfluidic polymeric devices. Electrokinetic injection is used to reproducibly introduce discrete femtolitre to picolitre volumes of charged lipids into a separation microchannel containing low (100 μM–10 mM) concentration tetraalkylammonium tetraphenylborate background electrolyte (BGE) in N -methylformamide, supporting rapid electro-osmotic fluid flow in polydimethylsiloxane microchannels. The quality of the resulting electrophoretic separations depends on the voltage and timing of the injection pulse, the BGE concentration and the electric field strength. Injected volumes increase with longer injection pulse widths and higher injection pulse amplitudes. Separation efficiency, as measured by total plate number, N , increases with increasing electric field and with decreasing BGE concentration. Electrophoretic separations of binary and ternary lipid mixtures were achieved with high resolution ( R s ∼ 5) and quality ( N > 7.7 × 10 6 plates m −1 ). Rapid in vivo monitoring of lipid biomarkers requires high-quality separation and detection of lipids downstream of microdialysis sample collection, and the multilayered non-aqueous microfluidic devices studied here offer one possible avenue to swiftly process complex lipid samples. The resulting capability may make it possible to correlate oxidative stress with in vivo lipid biomarker levels.
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Rozprawy doktorskie na temat "Electrophoresis microchip devices"

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Roychoudhury, Appan. "Biosensors and capillary electrophoresis microchip devices for analytical applications". Thesis, IIT, Delhi, 2019. http://eprint.iitd.ac.in:80//handle/2074/8069.

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Pagaduan, Jayson Virola. "Immunoassays of Potential Cancer Biomarkers in Microfluidic Devices". BYU ScholarsArchive, 2015. https://scholarsarchive.byu.edu/etd/5772.

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Laboratory test results are important in making decisions regarding a patient's diagnosis and response to treatment. These tests often measure the biomarkers found in biological fluids such blood, urine, and saliva. Immunoassay is one type of laboratory test used to measure the level of biomarkers using specific antibodies. Microfluidics offer several advantages such as speed, small sample volume requirement, portability, integration, and automation. These advantages are motivating to develop microfluidic platforms of conventional laboratory tests. I have fabricated polymer microfluidic devices and developed immunoassays on-chip for potential cancer markers. Silicon template devices were fabricated using standard photolithographic techniques. The template design was transferred to a poly(methyl methacrylate) (PMMA) piece by hot embossing and subsequently bonded to another PMMA piece with holes for reservoirs. I used these devices to perform microchip immunoaffinity electrophoresis to detect purified recombinant thymidine kinase 1 (TK1). Buffer with 1% methylcellulose acted as a dynamic coating that minimized nonspecific adsorption of protein and as sieving matrix that enabled separation of free antibody from antibody-TK1 complexes. Using this technique, I was able to detect TK1 concentration >80 nM and obtained separation results within 1 minute using a 5 mm effective separation length. Detection of endogenous TK1 in serum is difficult because TK1 is present at the pM range. I compared three different depletion methods to eliminate high abundance immunoglobulin and human serum albumin. Cibacron blue columns depleted abundant protein but also nonspecifically bound TK1. I found that ammonium sulfate precipitation and IgG/albumin immunoaffinity columns effectively depleted high abundance proteins. TK1 was salted out of the serum with saturated ammonium sulfate and still maintained activity. To integrate affinity columns in microfluidic devices, I have developed a fast and easy strategy for initial optimization of monolith affinity columns using bulk polymerization of multiple monolith solutions. The morphology, surface area, and porosity, were qualitatively assessed using scanning electron microscopy. This method decreased the time, effort, and resources compared to in situ optimization of monoliths in microfluidic devices. This strategy could be used when designing novel formulations of monolith columns. I have also integrated poly(ethylene glycol dimethacrylate-glycidyl methacrylate) monolith affinity columns in polymer microfluidic devices to demonstrate the feasibility of extracting human interleukin 8 (IL8), a cancer biomarker, from saliva. Initial results have shown that the affinity column (~3 mm) was successfully integrated into the devices without prior surface modification. Furthermore, anti-IL8 was immobilized on the surface of the monolith. Electrochromatograms showed that 1 ng/mL of IL8 can be detected when in buffer while 10 ng/mL was detected when IL8 was spiked in saliva. Overall, these findings can be used to further develop immunoassays in microfluidic platforms, especially for analyzing biological fluids.
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Jiang, Yutao. "A multi-reflection cell for enhanced absorbance detection in microchip-based capillary electrophoresis devices". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ40064.pdf.

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Beauchamp, Michael J. "3D Printed Microfluidic Devices for Bioanalysis". BYU ScholarsArchive, 2019. https://scholarsarchive.byu.edu/etd/8566.

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This work presents the development of 3D printed microfluidic devices and their application to microchip analysis. Initial work was focused on the development of the printer resin as well as the development of the general rules for resolution that can be achieved with stereolithographic 3D printing. The next stage of this work involved the characterization of the printer with a variety of interior and exterior resolution features. I found that the minimum positive and negative feature sizes were about 20 μm in either case. Additionally, micropillar arrays were printed with pillar diameters as small as 16 μm. To demonstrate one possible application of these small resolution features I created microfluidic bead traps capable of capturing 25 μm polystyrene particles as a step toward capturing cells. A second application which I pioneered was the creation of devices for microchip electrophoresis. I separated 3 preterm birth biomarkers with good resolution (2.1) and efficiency (3600 plates), comparable to what has been achieved in conventionally fabricated devices. Lastly, I have applied some of the unique capabilities of our 3D printer to a variety of other device applications through collaborative projects. I have created microchips with a natural masking monolith polymerization window, spiral electrodes for capacitively coupled contactless conductivity detection, and a removable electrode insert chip. This work demonstrates the ability to 3D print microfluidic structures and their application to a variety of analyses.
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Weldegebriel, Amos. "A UV detector for microfluidic devices". Thesis, Kansas State University, 2014. http://hdl.handle.net/2097/17626.

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Master of Science
Department of Chemistry
Christopher T. Culbertson
Chemical separation involves selective movement of a component out of a region shared by multiple components into a region where it is the major occupant. The history of the field of chemical separations as a concept can be dated back to ancient times when people started improving the quality of life by separation of good materials from bad ones. Since then the field of chemical separation has become one of the most continually evolving branches of chemical science and encompasses numerous different techniques and principles. An analytical chemist’s quest for a better way of selective identification and quantification of a component by separating it from its mixture is the cause for these ever evolving techniques. As a result, today there are numerous varieties of analytical techniques for the separation of complex mixtures. High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Capillary Electrophoresis (CE) and Gel Electrophoresis are a few out of a long list. Each these techniques manipulates the different physical and chemical properties of an analyte to achieve a useful separation and thus certain techniques will be suited for certain molecules. This work primarily focuses on the use of Capillary Electrophoresis as a separation technique. The mechanism of separation in Capillary Zone Electrophoresis and principles of UV detection will discussed in chapter one. Chapter two contains a discussion about the application of Capillary Electrophoresis (CE) on microfluidc devices. This will include sections on: microfabrication techniques of PDMS and photosensitized PDMS (photoPDMS), a UV detector for microfluidic devices and its application for the detection of wheat proteins. In Chapter three we report the experimental part of this project which includes; investigations on the effect of UV exposure time and thermal curing time on feature dimensions of photoPDMS microfluidic device, investigations on the injection and separation performances of the device, characterization of a UV detector set up and its application for the separation and detection of wheat gliadin proteins. The results of these investigations are presented in chapter four.
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Du, Fuying, i 杜富滢. "Microchip-capillary electrophoresis devices with dual-electrode detectors for determination of polyphenols, amino acids andmetabolites in wine and biofluids". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B48521693.

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The electrochemical detector provides a promising detection mode for capillary electrophoresis (CE) due to its excellent sensitivity, good portability, high selectivity, easy miniaturization, low capital and running cost. To widen its scope for determining trace analytes in complex samples, three dual-electrode detectors were fabricated to enable the determination of electro-inactive analytes, to assess co-eluted peaks and to give a large enhancement of the detection sensitivity by modifying electrode surface using multi-walled carbon nanotubes (MWNTs). To determine trace non-electroactive amino acids present in human tears, a serial dual-electrode detector was developed using an upstream on-capillary Pt film electrode to oxidize bromide to bromine at +1.0 V and a downstream Pt disk electrode to detect the residual bromine at +0.2 V after their reaction with amino acids eluted out from the separation capillary. The bromide reagent was introduced after CE separation by a newly designed coaxial post-column reactor fabricated onto the PMMA chip. Using optimized CE buffer containing 20 mM borate, 20 mM SDS at pH 9.8, L-glutamine, L-alanine and taurine were baseline separated with detection limits ranging from 0.56-0.65 μM and a working range of 2-200 μM for L-glutamine and of 2-300 μM for both L-alanine and taurine. Method reliability was established by close to 100% recoveries for spiked amino acids and good agreement between the measured and the literature reported amino acid concentrations in tears. For the determination of polyphenols in wine, a microchip-CE device was fabricated with a dual-opposite carbon fiber microelectrode operated in a parallel mode to assess peak purity. Under optimized conditions, (+)-catechin, trans-resveratrol, quercetin, (-)-epicatechin and gallic acid were baseline separated within 16 min with detection limits ranging from 0.031- 0.21 mg/L and repeatability of 2.0-3.3 % (n=5). The use of an opposite dual-electrode enables the simultaneous determination of peaks and measurement of their current ratios at +0.8 V and +1.0 V vs Ag/AgCl. The capability of using current ratio to identify the presence of co-migrating impurities was demonstrated in a mixed standard solution with overlapping (+)-catechin and (-)-epicatechin peaks and in a commercial red wine with interfering impurities. Matching of both the migration time and the current ratio reduce false positive and validate polyphenol quantitation in red wine. Lastly, a dual-opposite MWNTs modified carbon fiber microelectrode (CFME) was developed to determine the biomarkers (4-nitrophenol, 4-nitrophenyl-glucuronide and 4-nitrophenyl-sulfate) needed to assess exposure to methyl parathion. Use of the MWNTs modified CFME showed a much higher sensitivity than bare CFME, with a detection limit of 0.46 μM for 4-nitrophenol. Baseline separation of all three biomarkers was obtained within 31 min by a 45 cm long capillary under 12 kV in a 20 mM phosphate buffer at pH 7.0. The method developed was successfully utilized to determine low levels of biomarkers in human urine without using complex pretreatment steps and delivered recoveries ranging from 95.3 - 97.3% and RSDs within 5.8% (n=3). Using a parallel dual-electrode detector was shown to deliver reliable results with matching current ratios and comparable migration time to those obtained from biomarker standards.
published_or_final_version
Chemistry
Doctoral
Doctor of Philosophy
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Kumar, Suresh. "Design, Fabrication, and Optimization of Miniaturized Devices for Bioanalytical Applications". BYU ScholarsArchive, 2015. https://scholarsarchive.byu.edu/etd/5979.

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My dissertation work integrates the techniques of microfabrication, micro/nanofluidics, and bioanalytical chemistry to develop miniaturized devices for healthcare applications. Semiconductor processing techniques including photolithography, physical and chemical vapor deposition, and wet etching are used to build these devices in silicon and polymeric materials. On-chip micro-/nanochannels, pumps, and valves are used to manipulate the flow of fluid in these devices. Analytical techniques such as size-based filtration, solid-phase extraction (SPE), sample enrichment, on-chip labeling, microchip electrophoresis (µCE), and laser induced fluorescence (LIF) are utilized to analyze biomolecules. Such miniaturized devices offer the advantages of rapid analysis, low cost, and lab-on-a-chip scale integration that can potentially be used for point-of-care applications.The first project involves construction of sieving devices on a silicon substrate, which can separate sub-100-nm biostructures based on their size. Devices consist of an array of 200 parallel nanochannels with a height step in each channel, an injection reservoir, and a waste reservoir. Height steps are used to sieve the protein mixture based on size as the protein solution flows through channels via capillary action. Proteins smaller than the height step reach the end of the channels while larger proteins stop at the height step, resulting in separation. A process is optimized to fabricate 10-100 nm tall channels with improved reliability and shorter fabrication time. Furthermore, a protocol is developed to reduce the electrostatic interaction between proteins and channel walls, which allows the study of size-selective trapping of five proteins in this system. The effects of protein size and concentration on protein trapping behavior are evaluated. A model is also developed to predict the trapping behavior of different size proteins in these devices. Additionally, the influence of buffer ionic strength, which can change the effective cross-sectional area of nanochannels and trapping of proteins at height steps, is explored in nanochannels. The ionic strength inversely correlates with electric double layer thickness. Overall, this work lays a foundation for developing nanofluidic-based sieving systems with potential applications in lipoprotein fractionation, protein aggregate studies in biopharmaceuticals, and protein preconcentration. The second project focuses on designing and developing a microfluidic-based platform for preterm birth (PTB) diagnosis. PTB is a pregnancy complication that involves delivery before 37 weeks of gestation, and causes many newborn deaths and illnesses worldwide. Several serum PTB biomarkers have recently been identified, including three peptides and six proteins. To provide rapid analysis of these PTB biomarkers, an integrated SPE and µCE device is assembled that provides sample enrichment, on-chip labeling, and separation. The integrated device is a multi-layer structure consisting of polydimethylsiloxane valves with a peristaltic pump, and a porous polymer monolith in a thermoplastic layer. The valves and pump are fabricated using soft lithography to enable pressure-based sample actuation, as an alternative to electrokinetic operation. Porous monolithic columns are synthesized in the SPE unit using UV photopolymerization of a mixture consisting of monomer, cross-linker, photoinitiator, and various porogens. The hydrophobic surface and porous structure of the monolith allow both protein retention and easy flow. I have optimized the conditions for ferritin retention, on-chip labelling, elution, and µCE in a pressure-actuated device. Overall functionality of the integrated device in terms of pressure-controlled flow, protein retention/elution, and on-chip labelling and separation is demonstrated using a PTB biomarker (ferritin). Moreover, I have developed a µCE protocol to separate four PTB biomarkers, including three peptides and one protein. In the future, an immunoaffinity extraction unit will be integrated with SPE and µCE to enable rapid, on-chip analysis of PTB biomarkers. This integrated system can be used to analyze other disease biomarkers as well.
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Sonker, Mukul. "Electrokinetically Operated Integrated Microfluidic Devices for Preterm Birth Biomarker Analysis". BYU ScholarsArchive, 2017. https://scholarsarchive.byu.edu/etd/7001.

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Microfluidics is a vibrant and expanding field that has the potential for solving many analytical challenges. Microfluidics shows promise to provide rapid, inexpensive, efficient, and portable diagnostic solutions that can be used in resource-limited settings. Microfluidic devices have gained immense interest as diagnostic tools for various diseases through biomarker analysis. My dissertation work focuses on developing electrokinetically operated integrated microfluidic devices for the analysis of biomarkers indicative of preterm birth risk. Preterm birth (PTB), a birth prior to 37 weeks of gestation, is the most common complication of pregnancy and the leading cause of neonatal deaths and newborn illnesses. In this dissertation, I have designed, fabricated and developed several microfluidic devices that integrate various sample preparation processes like immunoaffinity extraction, preconcentration, fluorescent labeling, and electrophoretic separation of biomarkers indicative of PTB risk. I developed microchip electrophoresis devices for separation of selected PTB biomarkers. I further optimized multiple reversed-phase porous polymer monoliths UV-polymerized in microfluidic device channels for selective retention and elution of fluorescent dyes and PTB biomarkers to facilitate on-chip labeling. Successful on-chip fluorescent labeling of multiple PTB biomarkers was reported using these microfluidic devices. These devices were further developed using a pH-mediated approach for solid-phase extraction, resulting in a ~50 fold enrichment of a PTB biomarker. Additionally, this approach was integrated with microchip electrophoresis to develop a combined enrichment and separation device that yielded 15-fold preconcentration for a PTB peptide. I also developed an immunoaffinity extraction device for analyzing PTB biomarkers directly from a human serum matrix. A glycidyl methacrylate monolith was characterized within microfluidic channels for immobilization of antibodies to PTB biomarkers. Antibody immobilization and captured analyte elution protocols were optimized for these monoliths, and two PTB biomarker proteins were successfully extracted using these devices. This approach was also integrated with microchip electrophoresis for combined extraction and separation of two PTB biomarkers in spiked human serum in <30 min. In the future, these optimized microfluidic components can be integrated into a single platform for automated immunoaffinity extraction, preconcentration, fluorescent labeling, and separation of PTB biomarkers. This integrated microfluidic platform could significantly improve human health by providing early diagnosis of PTBs.
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Agostinelli, Simone. "A compartmentalised microchip platform with charged hydrogel to study protein diffusion for Single Cell Analysis". Master's thesis, Alma Mater Studiorum - Università di Bologna, 2020. http://amslaurea.unibo.it/20333/.

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Within one tumor, cancer cells exist as different sub-populations due to the variations in expression of crucial bio-markers. The prevalence of even minor cell sub-populations can determine overall cancer progression and treatment response. Single-cell protein analysis is a way to identify these cell sub-populations; therefore we developed a microfluidic platform with ultrahigh-sensitivity for single-cell protein analysis. As the key step to develop such a platform, protein migration under the application of an electric field has to be understood. COMSOL multi-physics software is used as a tool to understand the protein migration in microfluidic channels, which contain ion-selective hydrogels as the separation matrix. The objective of this thesis work, is to minimize the protein losses to diffusion and to maximize the fluorescent signal in order to quantify the protein expression in single cells. The novelty of this work lies in the use of ion-selective hydrogels to eliminate the diffusional losses and separate the proteins based on their mass and charge. This thesis project has been performed thanks to an Erasmus fellowship at MCS Department of the University of Twente.
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Lo, Chih-Cheng. "Dna electrophoresis in photopolymerized polyacrylamide gels on a microfluidic device". [College Station, Tex. : Texas A&M University, 2008. http://hdl.handle.net/1969.1/ETD-TAMU-2685.

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Książki na temat "Electrophoresis microchip devices"

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Fung, Ying Sing, Fuying Du, Wenpeng Guo, Tongmei Ma i Qidan Chen. Microfluidic Chip-Capillary Electrophoresis Devices. Taylor & Francis Group, 2015.

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Microfluidic Chip-Capillary Electrophoresis Devices. Taylor & Francis Group, 2015.

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Fung, Ying Sing, Fuying Du, Wenpeng Guo, Tongmei Ma i Qidan Chen. Microfluidic Chip-Capillary Electrophoresis Devices. Taylor & Francis Group, 2019.

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Fung, Ying Sing, Fuying Du, Wenpeng Guo, Tongmei Ma i Qidan Chen. Microfluidic Chip-Capillary Electrophoresis Devices. Taylor & Francis Group, 2015.

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Części książek na temat "Electrophoresis microchip devices"

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Shiddiky, Muhammad J. A., i Yoon-Bo Shim. "Microchip and Capillary Electrophoresis Using Nanoparticles". W Microfluidic Devices in Nanotechnology, 213–53. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2010. http://dx.doi.org/10.1002/9780470622551.ch6.

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Nielsen, Anna V., i Adam T. Woolley. "Device Fabrication and Fluorescent Labeling of Preterm Birth Biomarkers for Microchip Electrophoresis". W Methods in Molecular Biology, 175–84. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9213-3_12.

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Wu, Ruige, Zhiping Wang i Ying Sing Fung. "Multidimensional Microchip-Capillary Electrophoresis Device for Determination of Functional Proteins in Infant Milk Formula". W Methods in Molecular Biology, 111–18. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2353-3_10.

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Kwok, Yien C., Braden C. Giordano, Jerome P. Ferrance i James P. Landers. "Infrared-Mediated Thermocycling for DNA Amplification and Electrophoretic Separation on an Integrated Microchip Device". W Micro Total Analysis Systems 2002, 193–94. Dordrecht: Springer Netherlands, 2002. http://dx.doi.org/10.1007/978-94-010-0295-0_64.

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Schappler, Julie, Jean-Luc Veuthey i Serge Rudaz. "18 Coupling CE and microchip-based devices with mass spectrometry". W Capillary Electrophoresis Methods for Pharmaceutical Analysis, 477–521. Elsevier, 2008. http://dx.doi.org/10.1016/s0149-6395(07)00018-9.

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"Implementing Sample Preconcentration in Microfluidic Devices". W Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, 1397–440. CRC Press, 2007. http://dx.doi.org/10.1201/9781420004953-59.

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"Microfluidic Devices with Mass Spectrometry Detection". W Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, 1481–528. CRC Press, 2007. http://dx.doi.org/10.1201/9781420004953-62.

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"Implementing Sample Preconcentration in Microfluidic Devices". W Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, 1397–440. CRC Press, 2007. http://dx.doi.org/10.1201/9781420004953-59.

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"Microfluidic Devices with Mass Spectrometry Detection". W Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, 1481–528. CRC Press, 2007. http://dx.doi.org/10.1201/9781420004953-62.

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Legendre, Lindsay, James Landers i Jerome Ferrance. "Microfluidic Devices for Electrophoretic Separations". W Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition, 335–58. CRC Press, 2007. http://dx.doi.org/10.1201/9781420004953.ch10.

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Streszczenia konferencji na temat "Electrophoresis microchip devices"

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Jacobson, Stephen C., Christopher T. Culbertson i J. Michael Ramsey. "High Speed Microchip Electrophoresis". W Laser Applications to Chemical and Environmental Analysis. Washington, D.C.: Optica Publishing Group, 1998. http://dx.doi.org/10.1364/lacea.1998.lmb.3.

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Open tubular separations in the liquid phase including capillary electrophoresis are conventionally practiced in capillary tubes with diameters of tens of micrometers and lengths of tens of centimeters. Structures having similar dimensions can be fabricated on planar substrates using micromachining techniques. Microfabricated separation devices have been demonstrated for capillary electrophoresis,1 2 synchronized cyclic electrophoresis,3 and free-flow electrophoresis.4 The separation performance hinges upon minimizing the spatial extent of the injection plug and detector observation region, and optimizing the separation field strength. The injection plug width can be minimized by fabricating narrow channel dimensions for the injection valve, and the detector observation region can be minimized by having a small excitation volume or tight spatial filtering for fluorescence detection. For high speed separations, the separation column does not have to be long, e.g., < 1 mm long. Unfortunately, the footprint of these microfluidic devices is usually > 200 mm2, but the channel manifold can be designed such that the potential drop is small in areas not contributing to the separation. This results in a maximum field strength in the separation column. Because the resistance in the channel is proportional to the length and inversely proportional to the cross-sectional area, thin channels are fabricated for the injection valve and separation column, and wide channels for all other sections of the channel manifold.
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Takarada, Tohru, Yuzo Hamaguchi, Masako Ogawa i Mizuo Maeda. "Novel Affinity Microchip Electrophoresis for Gene Mutation Assay". W 2002 International Conference on Solid State Devices and Materials. The Japan Society of Applied Physics, 2002. http://dx.doi.org/10.7567/ssdm.2002.lc-1-2.

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"MICROCHIP CAPILLARY ELECTROPHORESIS DEVICE FOR AMPEROMETRIC DETECTION OF DNA WITH REDOX INTERCALATION". W International Conference on Biomedical Electronics and Devices. SciTePress - Science and and Technology Publications, 2011. http://dx.doi.org/10.5220/0003290802840287.

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Pennathur, Sumita, Fabio Baldessari, Mike Kattah, Paul J. Utz i Juan G. Santiago. "Electrophoresis in Nanochannels". W ASME 2006 2nd Joint U.S.-European Fluids Engineering Summer Meeting Collocated With the 14th International Conference on Nuclear Engineering. ASMEDC, 2006. http://dx.doi.org/10.1115/fedsm2006-98558.

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Micro- and nanofabrication technology enables the application of electrokinetics as a method of performing chemical analyses and achieving liquid pumping in electronically-controlled microchip systems with no moving parts. We are studying and leveraging the unique separation modalities offered by nanoscale electrokinetic channels. We report analytical, numerical, and experimental investigations of nanochannel electrophoretic transport and separation dynamics of neutral and charged analytes. Our study includes continuum-theory-based analytical and numerical studies of nanofluidic electrophoretic separation dynamics, as well as experimental validation of these models. We have used 40, 100, and 1,560 nm deep channels etched in fused silica to independently measure mobility and valence of small ions. We also use these devices to separate 10 to 100 base pair DNA in the absence of a gel separation matrix. The effective free-solution mobilities of the ds-DNA oligonucleotides measured in 1560 nm deep channel are consistent with reported literature values, while smaller values of the mobility were measured for 4o nm deep channels for the same charge-species. The goal of our work is to explore and exploit electrokinetic flow regimes with extreme scales of length and charge density.
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"CAPILLARY ELECTROPHORESIS ELECTROCHEMICAL DETECTOR WITH NOBLE MICROCHANNEL STRUCTURE FOR MINIATURIZATION - Development of a Capillary Electrophoresis Microchip Format Electrochemical Detector for Endocrine Disruptors Sensing". W International Conference on Biomedical Electronics and Devices. SciTePress - Science and and Technology Publications, 2008. http://dx.doi.org/10.5220/0001046901300133.

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Shao, Zhanjie, Carolyn L. Ren i Gerry E. Schneider. "Multi-Step Dynamic Control for Enhanced Electrokinetic Transport Characteristics in Microchip Capillary Electrophoresis". W ASME 2008 International Mechanical Engineering Congress and Exposition. ASMEDC, 2008. http://dx.doi.org/10.1115/imece2008-68831.

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A numerical model has been developed and is used to study the loading and dispensing processes in on-chip cross-linked microchannels. The electrokinetic transport characteristics and the roles of species’ electrophoretic mobilities and diffusion coefficients on the electrokinetic flow are revealed. A study is also performed on an implementation of multi-stage injection. The study of conventional one-step injection and separation is performed and helps construct a distinct understanding of the processes. Species movement and sample plug development with diffusion are examined; results include concentration profiles and contour plots over a range of injection and separation time. Real-time monitoring of different species’ movements is performed for injection guidance. Some limitations of the separation process are presented with potential solutions, such as the removable tail effect and exceptional quick diffusion. Using innovative dynamic control, efforts are made to control the flow and species transport for improved sample plugs, which is key to achieving excellent electrophoretic separation. Through a series of multi-step injection schemes, four typical sample plugs are produced with specific attributes such as reduced dispersion leakage, desirable sample plug size, enhanced shape, etc. Comparisons of conventional and the proposed methods are performed. Typical resulting sample plugs are evaluated using the two developed parameters of resolution and detectability for numerically simulated separation processes. Depending on requirements, one can generate some specific sample plugs through this multi-step dynamic injection method. The resulting understanding will assist in the design of microfluidic devices for separation by providing insight into the process influences and controls and by identifying areas for further research.
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Ohara, T., i A. Majumdar. "Ratcheting Electrophoresis Microchip (REM) for Programmable Transport and Separation of Macromolecules". W ASME 2001 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2001. http://dx.doi.org/10.1115/imece2001/mems-23888.

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Abstract This paper introduces the concept of a ratcheting electrophoresis microchip (REM), a microfluidic device for electrophoretic separation of macromolecules such as DNA and proteins in aqueous solution using low applied voltages (∼ 1 V). The device consists of several thousands of parallel linear electrodes with a constant pitch of about 10 μm. A spatial saw-tooth like potential distribution generated by the electrode array causes local electrophoretic migration of charged molecules between adjacent electrodes. By cycling the potential distribution in a certain pattern, the spatio-temporal electrophoretic ratchet can be used to separate and manipulate macromolecules at speeds much faster than thermal ratchets or more traditional techniques such as capillary or gel electrophoresis. This paper describes results of two simulations: First, using a simple one-dimensional potential distribution for the ratchet, the basic device function is examined using a probabilistic approach that simulates the interplay between electrophoretic mobility and molecular diffusion. The results suggest that the REM can function as a molecular filter through which only molecules having mobility larger than a threshold can pass. The REM can also be programmed to separate molecules to create a molecular profile, much like conventional electrophoresis. Second, two-dimensional stochastic simulations based on molecular diffusion and transient Debye screening by mobile ions are used to demonstrate the feasibility of the REM. The results suggest that biomolecular separation can indeed be achieved within time and length scales much shorter than capillary and gel electrophoresis.
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Dunphy, Katherine, Veljko Milanovic, Samantha Andrews, Taku Ohara i Arun Majumdar. "Rapid Separation and Manipulation of DNA by a Ratcheting Electrophoresis Microchip (REM)". W ASME 2002 International Mechanical Engineering Congress and Exposition. ASMEDC, 2002. http://dx.doi.org/10.1115/imece2002-33564.

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The Ratcheting Electrophoresis Microchip (REM) is a microfluidic device for electrophoretic separation of biomolecules such as DNA and proteins. By using thousands of electrodes along the length of a microchannel, the REM separates molecules using low applied voltages (∼1 V) in short times (&lt; 1 minute). This paper describes the microfabriation of the REM and initial testing results. Parallel arrays of platinum electrodes are fabricated on a silicon chip with a pitch of 10 μm. Two types of channels are fabricated: silicon nitride channels fabricated on the chip and poly(dimelthylsiloxane) (PDMS) channels fabricated separately and attached to the chip. Initial testing shows partial success with the PDMS channels and promis ing results for the silicon nitride channels.
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Ramsey, J. Michael. "Lab-on-a-Chip Devices: Thinking Small About Chemical and Biochemical Measurements". W Laser Applications to Chemical and Environmental Analysis. Washington, D.C.: Optica Publishing Group, 1998. http://dx.doi.org/10.1364/lacea.1998.lmb.1.

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Demonstrations of microfabricated chemical instrumentation initially focused on chemical separations, first in the gas phase and later in condensed phase using capillary electrophoresis. It soon became clear that microfabrication could be extended to include a much larger portion of the chemical measurement problem and the concept of the Lab-on-a-Chip resulted[1,2,3]. The realization of the Lab-on-a-Chip requires the development of a number of functional elements or unit processes that can eventually be integrated to solve chemical and biochemical measurement problems. As the field of micromachined chemical instrumentation continues to grow, more sophisticated devices with increased functionality are being fabricated and tested. Functional elements that have been demonstrated for chemical and biochemical analysis include free solution electrophoresis [4,5,6,7,8,9], electrophoretic sizing of DNA fragments [11,12], and methods for isolation of neutral species [13,14,15]. In addition, initial levels of monolithic integration have been demonstrated by coupling chemical separations with chemical and enzymatic reactions [16,17,18,19,20]. Quantification tools used for detection of materials on or from microchips have primarily included fluorescence which is particularly applicable due to its extreme sensitivity[21] and electrospray ionization for off-chip analysis using mass spectrometry [22]. In addition to these more conventional chemical analysis methods, electrokinetically driven fluidics have been used to perform electrokinetically focused flow cytometry [23] and homogeneous enzyme reaction kinetics [24]. These latter two capabilities are described in greater detail below.
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Fister, J. C., L. M. Davis, S. C. Jacobson i J. M. Ramsey. "High Sensitivity Detection on Microchips". W Laser Applications to Chemical and Environmental Analysis. Washington, D.C.: Optica Publishing Group, 1996. http://dx.doi.org/10.1364/lacea.1996.lwd.6.

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High efficiency separations coupled with rapid response times have been demonstrated on capillary electrophoresis devices micromachined on glass substrates. [1-3] Although detection of single organic dyes has been achieved with fluorescence detection in capillaries, [4] such detection limits have not yet been demonstrated in micromachined channels. Efficient, single molecule detection is desirable for many potential applications such as rapid DNA sequencing. The structure of microfabricated separation channels, however, does not readily facilitate a 90° fluorescence excitation/collection geometry which has been used to achieve high sensitivities in capillaries. [4] This optical geometry allows efficient spatial rejection of scattering at the capillary solution interfaces. Confocal detection in which the excitation source is introduced through the collection optics provides a means of achieving both high axial resolution and high collection efficiency.
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