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Artykuły w czasopismach na temat "ECF σ factors"

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Guzina, Jelena, та Marko Djordjevic. "Promoter Recognition by Extracytoplasmic Function σ Factors: Analyzing DNA and Protein Interaction Motifs". Journal of Bacteriology 198, № 14 (2016): 1927–38. http://dx.doi.org/10.1128/jb.00244-16.

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ABSTRACTExtracytoplasmicfunction (ECF) σ factors are the largest and the most diverse group of alternative σ factors, but their mechanisms of transcription are poorly studied. This subfamily is considered to exhibit a rigid promoter structure and an absence of mixing and matching; both −35 and −10 elements are considered necessary for initiating transcription. This paradigm, however, is based on very limited data, which bias the analysis of diverse ECF σ subgroups. Here we investigate DNA and protein recognition motifs involved in ECF σ factor transcription by a computational analysis of canon
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Fang, Chengli, Lingting Li, Liqiang Shen, et al. "Structures and mechanism of transcription initiation by bacterial ECF factors." Nucleic Acids Research 47, no. 13 (2019): 7094–104. http://dx.doi.org/10.1093/nar/gkz470.

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Abstract Bacterial RNA polymerase (RNAP) forms distinct holoenzymes with extra-cytoplasmic function (ECF) σ factors to initiate specific gene expression programs. In this study, we report a cryo-EM structure at 4.0 Å of Escherichia coli transcription initiation complex comprising σE—the most-studied bacterial ECF σ factor (Ec σE-RPo), and a crystal structure at 3.1 Å of Mycobacterium tuberculosis transcription initiation complex with a chimeric σH/E (Mtb σH/E-RPo). The structure of Ec σE-RPo reveals key interactions essential for assembly of E. coli σE-RNAP holoenzyme and for promoter recognit
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Thakur, Krishan Gopal, Anagha Madhusudan Joshi та B. Gopal. "Structural and Biophysical Studies on Two Promoter Recognition Domains of the Extra-cytoplasmic Function σ Factor σC from Mycobacterium tuberculosis". Journal of Biological Chemistry 282, № 7 (2006): 4711–18. http://dx.doi.org/10.1074/jbc.m606283200.

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σ factors are transcriptional regulatory proteins that bind to the RNA polymerase and dictate gene expression. The extracytoplasmic function (ECF) σ factors govern the environment dependent regulation of transcription. ECF σ factors have two domains σ2 and σ4 that recognize the -10 and -35 promoter elements. However, unlike the primary σ factor σA, the ECF σ factors lack σ3, a region that helps in the recognition of the extended -10 element and σ1.1, a domain involved in the autoinhibition of σA in the absence of core RNA polymerase. Mycobacterium tuberculosis σC is an ECF σ factor that is ess
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Ho, Theresa D., та Craig D. Ellermeier. "PrsW Is Required for Colonization, Resistance to Antimicrobial Peptides, and Expression of Extracytoplasmic Function σ Factors in Clostridium difficile". Infection and Immunity 79, № 8 (2011): 3229–38. http://dx.doi.org/10.1128/iai.00019-11.

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ABSTRACTClostridium difficileis an anaerobic, Gram-positive, spore-forming, opportunistic pathogen that is the most common cause of hospital-acquired infectious diarrhea. In numerous pathogens, stress response mechanisms are required for survival within the host. Extracytoplasmic function (ECF) σ factors are a major family of signal transduction systems, which sense and respond to extracellular stresses. We have identified threeC. difficileECF σ factors. These ECF σ factors, CsfT, CsfU, and CsfV, induce their own expressions and are negatively regulated by their cognate anti-σ factors, RsiT, R
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Luo, Yun, Kei Asai, Yoshito Sadaie та John D. Helmann. "Transcriptomic and Phenotypic Characterization of a Bacillus subtilis Strain without Extracytoplasmic Function σ Factors". Journal of Bacteriology 192, № 21 (2010): 5736–45. http://dx.doi.org/10.1128/jb.00826-10.

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ABSTRACT Bacillus subtilis encodes seven extracytoplasmic function (ECF) σ factors. Three (σM, σW, and σX) mediate responses to cell envelope-active antibiotics. The functions of σV, σY, σZ, and σYlaC remain largely unknown, and strong inducers of these σ factors and their regulons have yet to be defined. Here, we define transcriptomic and phenotypic differences under nonstress conditions between a strain carrying deletions in all seven ECF σ factor genes (the Δ7ECF mutant), a ΔMWX triple mutant, and the parental 168 strain. Our results identify >80 genes as at least partially dependent on
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Ho, Theresa D., Jessica L. Hastie, Peter J. Intile та Craig D. Ellermeier. "The Bacillus subtilis Extracytoplasmic Function σ Factor σVIs Induced by Lysozyme and Provides Resistance to Lysozyme". Journal of Bacteriology 193, № 22 (2011): 6215–22. http://dx.doi.org/10.1128/jb.05467-11.

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Bacteria encounter numerous environmental stresses which can delay or inhibit their growth. Many bacteria utilize alternative σ factors to regulate subsets of genes required to overcome different extracellular assaults. The largest group of these alternative σ factors are the extracytoplasmic function (ECF) σ factors. In this paper, we demonstrate that the expression of the ECF σ factor σVinBacillus subtilisis induced specifically by lysozyme but not other cell wall-damaging agents. A mutation insigVresults in increased sensitivity to lysozyme killing, suggesting that σVis required for lysozym
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Luo, Yun, та John D. Helmann. "Extracytoplasmic Function σ Factors with Overlapping Promoter Specificity Regulate Sublancin Production in Bacillus subtilis". Journal of Bacteriology 191, № 15 (2009): 4951–58. http://dx.doi.org/10.1128/jb.00549-09.

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ABSTRACT Bacillus subtilis harbors seven extracytoplasmic function (ECF) σ factors. At least three ECF σ factors (σM, σW, and σX) are induced by, and provide resistance to, antibiotics and other agents eliciting cell envelope stress. Here, we report that ECF σ factors also contribute to antibiotic production. B. subtilis 168 strains that are lysogenic for the SPβ bacteriophage produce sublancin, which inhibits the growth of other, nonlysogenic strains. Genetic studies demonstrate that synthesis of sublancin is largely dependent on σX, with a smaller contribution from σM. A sigM sigX double mut
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Wecke, Tina, Birgit Veith, Armin Ehrenreich, and Thorsten Mascher. "Cell Envelope Stress Response in Bacillus licheniformis: Integrating Comparative Genomics, Transcriptional Profiling, and Regulon Mining To Decipher a Complex Regulatory Network." Journal of Bacteriology 188, no. 21 (2006): 7500–7511. http://dx.doi.org/10.1128/jb.01110-06.

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ABSTRACT The envelope is an essential structure of the bacterial cell, and maintaining its integrity is a prerequisite for survival. To ensure proper function, transmembrane signal-transducing systems, such as two-component systems (TCS) and extracytoplasmic function (ECF) σ factors, closely monitor its condition and respond to harmful perturbations. Both systems consist of a transmembrane sensor protein (histidine kinase or anti-σ factor, respectively) and a corresponding cytoplasmic transcriptional regulator (response regulator or σ factor, respectively) that mediates the cellular response t
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Mascher, Thorsten, Anna-Barbara Hachmann та John D. Helmann. "Regulatory Overlap and Functional Redundancy among Bacillus subtilis Extracytoplasmic Function σ Factors". Journal of Bacteriology 189, № 19 (2007): 6919–27. http://dx.doi.org/10.1128/jb.00904-07.

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ABSTRACT Bacillus subtilis encodes seven extracytoplasmic function (ECF) σ factors that regulate partially overlapping regulons related to cell envelope homeostasis and antibiotic resistance. Here, we investigated their physiological role by constructing a mutant set of single, double, triple, and quadruple ECF σ factor deletions in the undomesticated B. subtilis strain NCIB3610. This mutant set was subsequently screened for defects in motility, multicellular differentiation, and sensitivity to more than 200 chemicals by using Phenotype MicroArrays. A quadruple mutant strain, harboring deletio
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de Dios, Rubén, Eduardo Santero та Francisca Reyes-Ramírez. "Extracytoplasmic Function σ Factors as Tools for Coordinating Stress Responses". International Journal of Molecular Sciences 22, № 8 (2021): 3900. http://dx.doi.org/10.3390/ijms22083900.

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The ability of bacterial core RNA polymerase (RNAP) to interact with different σ factors, thereby forming a variety of holoenzymes with different specificities, represents a powerful tool to coordinately reprogram gene expression. Extracytoplasmic function σ factors (ECFs), which are the largest and most diverse family of alternative σ factors, frequently participate in stress responses. The classification of ECFs in 157 different groups according to their phylogenetic relationships and genomic context has revealed their diversity. Here, we have clustered 55 ECF groups with experimentally stud
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