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Artykuły w czasopismach na temat "Eagles minimal essential medium"
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Cinatl, J., J. Cinatl, V. Gerein, B. Kornhuber i H. W. Doerr. "The establishment and characterization of mouse L-929 cells in protein-free Eagle's Minimal Essential Medium". Journal of Biological Standardization 16, nr 4 (styczeń 1988): 249–57. http://dx.doi.org/10.1016/0092-1157(88)90012-1.
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Hnat, Michael, i Roger E. Bawdon. "Transfer of Meropenem in the ex Vivo Human Placenta perfusion Model". Infectious Diseases in Obstetrics and Gynecology 13, nr 4 (2005): 223–27. http://dx.doi.org/10.1155/2005/961356.
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Objectives.To determine maternal-fetal transplacental passage of meropenem in the ex vivo human perfusion model.Study design.Term placentae (n= 6) were collected immediately after delivery. A single cotyledon was localized, perfused and stabilized with physiologic Eagles minimal essential medium containing 3% bovine albumin and heparin as described by Chalier (Chalier JC. Criteria for evaluating perfusion experiments and presentation results. Contrib Gynecol Obstet 1985; 13:32–39). Meropenem was added to the maternal medium in concentrations similar to maternal serum peak and trough levels, then perfused through the maternal circulation of the cotyledon. To assess transfer and accumulation, fluid aliquots from both the maternal and fetal compartments were collected over an hour at defined intervals in an open and closed system. AntipyrineC14was added to the medium in order to calculate the transport fraction and clearance indexes. Meropenem and antipyrineC14concentrations were determined by High-pressure Liquid Chromatography and liquid scintillation, respectively.Results.Mean antipyrine transport fraction was 2.33 + 0.25. Maternal and fetal mean meropenem peak concentrations were 54.3 + 3.3μg/ml and 2.2 + 0.18μg/ml, respectively. Whereas, maternal and fetal mean trough concentrations were 12.7 + 1.3μg/ml and 0.41 + 0.10μg/ml, respectively. Mean peak clearance index was 0.077 + 0.007 and the mean trough was 0.052 + 0.015. Mean accumulation for the peak and trough concentrations of meropenem were 0.9 and 2.95μg/ml, respectively.Conclusions.Transplacental passage of meropenem was incomplete in the ex vivo human placental perfusion model. Accumulation was also noted in the fetal compartment
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Seino, Satoshi, Yasuo Imoto, Tomoya Kosaka, Tomoki Nishida, Takashi Nakagawa i Takao A. Yamamoto. "Antiviral Activity of Silver Nanoparticles Immobilized onto Textile Fabrics Synthesized by Radiochemical Process". MRS Advances 1, nr 11 (2016): 705–10. http://dx.doi.org/10.1557/adv.2016.43.
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ABSTRACTAntiviral activity of metallic Ag nanoparticles immobilized on textile fabrics were investigated. The Ag nanoparticles synthesized by radiochemical process are firmly immobilized on the surface of support textile fabrics of cotton. Small Ag particles of about 2–4 nm were observed together with relatively large particles of more than 10 nm. The Ag nanoparticles showed antiviral activity against Influenza A and Feline Calicivirus. The antiviral activity significantly depended on the concentration of the Eagle’s minimal essential medium. It was implied that the surface passivation by inhibitory agent lead to the deactivation of metallic Ag nanoparticles.
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Tung, L. C. "Immunocytochemistrical Studies on Cytoskeletal Change in Cultured Cardiomyocyte of Tilapia". Microscopy and Microanalysis 6, S2 (sierpień 2000): 888–89. http://dx.doi.org/10.1017/s143192760003693x.
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In the present study, the myofibril regeneration in the long-term cultured fish cardiomyocytes was studied with immunocytochemistry.Adult Tilapia heart was dissociated into a single-cell suspension with collagenase and protease-minced tissue method. The culture medium was Eagle's minimal essential medium (MEM) with Earle's salts, supplemented with 10% fetal calf serum, 1 x nonessential amino acid mixture, 100 IU/ml penicillin G, and 100 μg/ml streptomycin. The cultured cells were grown in a humidified CO2 incubator at 28°Cand in a medium without glutamine for eliminating fibroblast contamination. In the initial 24 h culture, the elongated-shape cells gradually shortened from their both ends and rounded up. Over 5 to 6 days postcultivation, the cells attached to the bottom of the culture flask and began to protrude pseudopodia. The cells could not be subcultured and also proliferated indefinitely. The life span of cells in culture was 30 to 60 days.
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Heredia, Nelly Solfania, Ann Sabrina Ávila i Luz Elena Velásquez. "In vitro culture of L3 larvae of nematodes obtained from the African giant snail Lissachatina fulica (Mollusca: Gastropoda) in Santa Fe de Antioquia". Biomédica 38 (1.08.2018): 24–29. http://dx.doi.org/10.7705/biomedica.v38i3.3408.
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Introducción. Más de 170 municipios colombianos están invadidos por Lissachatina fulica, caracol africano que puede portar larvas de nematodos de interés en salud humana y veterinaria. Los parásitos entran al caracol huésped intermediario en el estadio de larva L1, y allí cambian a L2 y L3, formas estas capaces de infectar a vertebrados.Objetivo. Estandarizar el cultivo in vitro de las L3 portadas por especímenes de L. fulica recolectados en Santa Fe de Antioquia.Materiales y métodos. Entre julio y noviembre de 2014 se recolectaron 10 caracoles, se sacrificaron y se digirieron con ácido clorhídrico al 0,7 %. Las larvas se recuperaron mediante la técnica de Baermann; se cultivaron 36 días en los medios Schneider, mínimo esencial de Eagle modificado por Dulbecco (Dulbecco’s Modified Eagles Minimal Essential Medium, DMEM), y Roswell Park Memorial Institute (RPMI), con suero fetal bovino (SFB) al 20 % y sin este, y agua destilada con SFB al 20 %. Los medios de cultivo se cambiaron cada 36 horas. Las larvas se midieron con el microscopio utilizando reglilla ocular, y se evaluaron la supervivencia, la longitud y el ancho. Se calcularon datos estadísticos de resumen y se hicieron gráficos de cajas y bigotes, así como la prueba t de Student. El nivel de significación (p) se estableció como menor de 0,05.Resultados. El 50 % de las larvas sobrevivió, 85 % en DMEM con SFB al 20 %, el 70 % con RPMI más SFB al 20 %, el 60 % en RPMI, el 50 % en Schneider más SFB al 20 %, el 45 % en Schneider y el 40 % en DMEM. El control sobrevivió diez días. Hubo diferencias significativas entre la longitud inicial promedio de las larvas y la longitud final promedio en los medios con suplementos: inicial, 645,83 μm; final en DMEM más SFB al 20 %, 732,65 μm (p<0,001); en RPMI más SFB al 20 %, 718,79 μm (p<0,001), y en Schneider más SFB al 20 %, 696,12 μm (p<0,01). No hubo diferencias significativas entre la anchura inicial promedio, de 24,99 μm, y la final.Conclusiones. El mejor medio para cultivar las L3 de L. fulica fue el DMEM más SFB al 20 %. En la evaluación del crecimiento larval, la longitud fue más informativa que la anchura. Las larvas estudiadas no correspondieron a Angiostrongylus cantonensis, A. costaricensis ni Aelurostrongylus abstrusus.
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Mouat, M. F., A. C. Cantrell i K. L. Manchester. "Membrane potential of rat hepatoma cells in culture: Influence of factors affecting amino acid transport". Bioscience Reports 15, nr 4 (1.08.1995): 173–84. http://dx.doi.org/10.1007/bf01540451.
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The effect has been studied of various media, hormones and of amino acids on the membrane potential of rat hepatoma cells in culture measured by microelectrode impalement. Cells in Eagle's minimal essential medium plus 5% serum had a value which varied daily from about 5–8 mV, inside negative. The membrane potential of rat hepatocytes was measured to be 8.7 ± 0.2mV, inside negative. The membrane potential of the hepatoma cells was decreased by insulin and increased by glucagon. Membrane potential was unaffected by change of medium to Hanks' or Earle's balanced salt solutions or deprivation of serum. It was, however, reduced in cells in phosphate-buffered saline and by reduction of pH. The former effect was shown to be due to the higher [Na+] of phosphat-buffered saline as opposed to the other media. Addition of alanine, glycine, serine, proline and methylaminoisobutyrate all reduced membrane potential by 2–3 mV. Smaller decreases were seen with methionine, leucine and phenylalanine, but none with glutamine, threonine, BCH (2-aminonorborane-2-carboxylic acid) and D-alanine. The results are compared with the effects of similar conditions on aminoisobutyrate uptake. Whilst there was a correlation under some conditions there was not under others. It is concluded that for the hepatoma cells factors additional to the membrane potential must exert some influence on the capacity for amino acid transport.
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Al-Nazhan, Saad, i Afaf Al-Nasser. "Viability of Human Periodontal Ligament Fibroblasts in Tissue Culture After Exposure to Different Contact Lens Solutions". Journal of Contemporary Dental Practice 7, nr 4 (2006): 37–44. http://dx.doi.org/10.5005/jcdp-7-4-37.
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Abstract Aim The viability of the periodontal ligament (PDL) cells is critical for successful healing of replanted avulsed teeth. Viability is primarily dependent on the duration of the extra-alveolar time and storage medium used to preserve teeth. Several storage media have been suggested but milk ranks highest. It would be desirable to evaluate other media as a suitable alternative for milk. The purpose of this study was to determine the viability of human PDL fibroblasts and their morphology after storage in different types of contact lens solutions. Methods and Materials PDL fibroblasts were cultured from a healthy extracted impacted human tooth and exposed to Bausch and Lomb (Renu), Ciba Vision (Titmus), and Alcon (Opti-free) contact lens solutions. Eagle's minimal essential medium served as control. The experiment was performed in plastic tissue culture clusters containing 24 wells. The PDL fibroblasts were grown in each well for three days. On the day of the experiment the culture medium was decanted, the cells were washed with phosphate buffered saline solution (PBS), and 1 ml of the tested solution was placed in each culture well. All tissue culture clusters were incubated at 37°C in 5% CO2 and 95% air for one, four, and 24 hrs. At the end of the incubation period, the cells were fixed and prepared for scanning electron microscope (SEM) examination. Results The results indicated Renu and Opti-free solutions were superior to Titmus solution in terms of their capacity to maintain the viability and normal morphology of PDL fibroblasts. Conclusion Contact lens solution is a good storage medium to maintain the viability of PDL fibroblasts for a short-term period. Citation Al-Nazhan S, Al-Nasser A. Viability of Human Periodontal Ligament Fibroblasts in Tissue Culture After Exposure to Different Contact Lens Solutions. J Contemp Dent Pract 2006 September;(7)4:037-044.
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Kellermayer, Miklós S. Z., Tamás Henics, György Szücs i Gerald H. Pollack. "Electron microscopic analysis of the microtubular structure of HEp-2 cells". Proceedings, annual meeting, Electron Microscopy Society of America 48, nr 3 (12.08.1990): 470–71. http://dx.doi.org/10.1017/s0424820100159898.
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The HEp-2 cell line was first established by Moore, et al. and today it serves as a widely used subject for a large variety of microbiological and cell-biological experiments. A tubular structure of unknown origin and function has been described first in virally infected, and later in uninfected HEp-2 cells. However, this tubular structure has not been further analyzed. We performed transmission (TEM) and whole cell mount electron microscopic studies of monolayer HEp-2 cells to morphologically describe the structure and relate it to other organelles of the cell.For embedding, HEp-2 cells were grown on glass coverslips in Eagle's minimal essential medium. 24 hours after passage, three types of procedures were carried out: (1) The cells were fixed with 2.5% glutaraldehyde in 0.14 M Na-cacodylate buffer. (2) The cells were treated for 10 min. with Hank's solution containing 0.2% Brij-58 and 2.5% glutaraldehyde. Fixation was completed with 2.5% glutaraldehyde in 0.14 M Na-cacodylate. (3) Cells were treated with 0.2% Brij-58 in Hank’s solution for 10 min. Subsequently, they were fixed with 2.5% glutaraldehyde in 0.14 M Na-cacodylate.
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Tifrea, Delia F., Pooja Ralli-Jain, Sukumar Pal i Luis M. de la Maza. "Vaccination with the Recombinant Major Outer Membrane Protein Elicits Antibodies to the Constant Domains and Induces Cross-Serovar Protection against Intranasal Challenge with Chlamydia trachomatis". Infection and Immunity 81, nr 5 (11.03.2013): 1741–50. http://dx.doi.org/10.1128/iai.00734-12.
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ABSTRACTTo determine the ability of the major outer membrane protein (MOMP) to elicit cross-serovar protection, groups of mice were immunized by the intramuscular (i.m.) and subcutaneous (s.c.) routes with recombinant MOMP (rMOMP) fromChlamydia trachomatisserovars D (UW-3/Cx), E (Bour), or F (IC-Cal-3) orChlamydia muridarumstrain Nigg II using CpG-1826 and Montanide ISA 720 VG as adjuvants. Negative-control groups were immunized i.m. and s.c. withNeisseria gonorrhoeaerecombinant porin B (Ng-rPorB) or i.n. with Eagle's minimal essential medium (MEM-0). Following vaccination, the mice developed antibodies not only against the homologous serovar but also against heterologous serovars. The rMOMP-vaccinated animals also mounted cell-mediated immune responses as assessed by a lymphoproliferative assay. Four weeks after the last immunization, mice were challenged i.n. with 104inclusion-forming units (IFU) ofC. muridarum. The mice were weighed for 10 days and euthanized, and the number of IFU in their lungs was determined. At 10 days postinfection (p.i.), mice immunized with the rMOMP ofC. muridarumorC. trachomatisD, E, or F had lost 4%, 6%, 8%, and 8% of their initial body weight, respectively, significantly different from the negative-control groups (Ng-rPorB, 13%; MEM-0, 19%;P< 0.05). The median number of IFU recovered from the lungs of mice immunized withC. muridarumrMOMP was 0.13 × 106. The median number of IFU recovered from mice immunized with rMOMP from serovars D, E, and F were 0.38 × 106, 7.56 × 106, and 11.94 × 106IFU, respectively. All the rMOMP-immunized animals had significantly less IFU than theNg-rPorB (40 × 106)- or MEM-0 (70 × 106)-immunized mice (P< 0.05). In conclusion, vaccination with rMOMP can elicit protection against homologous and heterologousChlamydiaserovars.
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Reader, S. C. J., B. Davison, J. G. Ratcliffe i W. R. Robertson. "Measurement of low concentrations of bovine thyrotrophin by iodide uptake and organification in porcine thyrocytes". Journal of Endocrinology 106, nr 1 (lipiec 1985): 13—NP. http://dx.doi.org/10.1677/joe.0.1060013.
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ABSTRACT Thyrocytes isolated from porcine thyroids by mechanical and enzymatic dispersion and cultured in Eagle's minimal essential medium, supplemented with 5% (v/v) fetal calf serum, glutamine and cortisol, formed a continuous monolayer within 48 h. This monolayer was without cytochemical peroxidase and diaphorase (NADPH reoxidation) activity. In the presence of bovine thyrotrophin (bTSH; 50 mu./l) the cells developed a follicular-like architecture which was maximal at 4 days before reverting back to a uniform monolayer at 6 days. There were no detectable changes in the total DNA content over this period. The follicular structures had marked diaphorase and peroxidase activity, the latter being apically distributed. Concomitant with follicle formation bTSH induced uptake and organification of iodide presented to the cells during the last 6 h of culture. The extent of this process depended on the dose of bTSH and the duration of stimulation. The most sensitive effects for both iodide uptake and organification occurred with 1 mu. bTSH/1 and were maximal with 100 mu./l. Uptake and organification were increased 20±8-fold and 9·6±2- fold (n = 10) respectively over the control with 100 mu./l and the doses of bTSH at which a half maximal response was seen (ED50) were 15±2 and 7±1 (s.d) mu./l (n = 10) respectively. On changing the culture medium to a serum-free system using HB101 culture medium the stimulation time for the most sensitive bTSH effect was reduced to 2·5 days. Moreover the sensitivity of iodide uptake to bTSH was dramatically increased being significantly stimulated by 0·1 mu./l, saturated with 10 mu./l, and having an ED50 of 7 ± 0·2 mu. bTSH/1 (n = 7). Over the dose range 0·1–10 mu. bTSH/l intra- and interassay coefficients of variation were 7%±1·5 (n = 15) and 11% ± 2·5 (n = 15) respectively. Cyclic AMP production in cultures incubated under similar conditions (i.e. after chronic TSH stimulation) was also stimulated by bTSH doses in the range 15·6–125 mu./l. Cells stimulated for 2·5 days with dibutyryl cyclic AMP (range 1 μmol/1–2 mmol/l) also actively concentrated iodide in a dose-dependent fashion. The presence of normal human serum in the medium yielded a progressive and dose-related decrease in TSH-stimulated iodide uptake. This inhibitory effect occurred with serum concentrations as low as 0·1%. In conclusion, a porcine cell culture system is described in which iodide uptake is significantly stimulated by 0·1 mu. bTSH/l, a sensitivity inferior only to that reported with the cytochemical bioassay. J. Endocr. (1985) 106, 13–20
Rozprawy doktorskie na temat "Eagles minimal essential medium"
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Lei, Jie. "The role of antioxidants in the hydrogen peroxide-induced opacification of sheep lens". Master's thesis, Lincoln University. Agriculture and Life Sciences Division, 2006. http://theses.lincoln.ac.nz/public/adt-NZLIU20070517.162145/.
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The lens of the eye needs to be transparent with a high refractive index to focus images on the retina. In cataracts the lens becomes opaque, eventually leading to blindness. There are many possible causes of cataract but a lot of evidence implicates oxidative damage as contributing to opacification. This includes epidemiological studies showing that diets rich in antioxidants lowered the prevalence of cataract. This research tested the hypothesis that if cataracts were at least partially caused by oxidative damage then their progression would be slowed by application of antioxidants. The antioxidants used were two plant compounds found in the diet, resveratrol and quercetin. The system used was sheep lenses cultured in Eagles Minimal Essential Medium (EMEM). Lenses remained transparent for up to 7 days in EMEM but became opaque within 24 h when exposed to 1 mM hydrogen peroxide (H2O2). The lens is exposed to H2O2 in vivo as it is found in the aqueous humor. Prior Lenses pre-treated with quercetin reduced but did not prevent opacification. Lens cell death, as determined by measurement of leakage of lactate dehydrogenase, was found to increase with H2O2 and the increase was prevented by pre-treatment with antioxidants. The role of the endogenous antioxidant glutathione was also investigated. It was found that H2O2 decreased the amount of reduced glutathione in the lens cortex and increased the levels of oxidised glutathione but only at levels of 2 mM and above. Thus the results of this research indicate that H2O2 at low concentration (1 mM) is able to damage lens cells and cause opacification without affecting the reduced glutathione levels and that the exogenous antioxidants have some ability to protect the lens.