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Najim, Najim Hadi. "Survival Enterohaemorrhagic E.coli O157in locally produce soft cheese in Baghdad city and studying the effect of some physical factors on its viability". Iraqi Journal of Veterinary Medicine 32, nr 2 (31.12.2008): 108–19. http://dx.doi.org/10.30539/iraqijvm.v32i2.744.
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A total of 24 samples of three different kinds of locally produced softcheese manufactured from raw milk of Cows , Buffaloes and Ewes( 8 samples of each) were collected at weekly intervals from differentareas of Baghdad city in the period from the beginning of December2007 till the end of January 2008.The main objective of this study was to isolats , identify andenumerate Enterohaemorrhagic E.coli O157from locally produced softcheese , besides that , to study the effect of cheese storage temperature(4 C) and different salt concentration solutions on the viability andsurvival of E.coli O157 in stored cheese .Data demonstrate that 11 out 24 of samples 45.84% were found tohave Enterohaemorrhagic E.coli O157 and their average counts were1.6× 104 CFU/gm.The result of statistical analysis demonstrate that cheese samplesmanufactured from cows raw milk had significantly ( P> 0.05) higherE.coli O157 counts and isolation rates over both cheese samples thatwere manufactured from Ewes and buffaloes raw milk .the average count of Enterohaemorrhagic E.coli O157 in cheesemanufactured from cows milk were 3.8× 104 CFU/gm and the isolationpercentage was 75% while their average counts in cheese manufacturedfrom Ewes and buffaloes milk were 7.3 ×103 & 2.4 ×103 CFU/gmrespectively and the isolation percentages were 37.5 % & 12.5%respectively.Data revealed that all soft cheese samples that were found to haveEnterohaemorrhagic E.coli O157 and stored at refrigeration temperature(4 C) had non significant ( P<0.05) reduction in the average counts ofE.coli O157after 3 weeks of storage and this indicate that the refrigerationstorage temperature (4 C) had no significant effect on the viabilityand survival of E.col O157 .All soft cheese samples that were found tohave Enterohaemorrhagic E.coli O157 were stored in brinesolutions at different salt concentrations such as 2.5%, 5%,7% and10% for 24 hr,1 week, 2weeks & 3weeks . Data revealed that brinesolution at high concentration of salt such as 10% had nonsignificant effect on the viability and survival of E.coli O157after 3weeks of storage.
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Jayanti, Devi Dwi, R. Susanti, Ari Yuniastuti i I. Wayan Suardana. "Deteksi Escherichia coli O157 pada air minum di Kelurahan Sekaran Gunungpati Semarang". Jurnal Biologi Udayana 24, nr 2 (25.12.2020): 55. http://dx.doi.org/10.24843/jbiounud.2020.v24.i02.p01.
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Penelitian bertujuan untuk mendeteksi adanya bakteri Escherichia coli O157 pada air minum kemasan, air minum isi ulang, dan air sumur di Kelurahan Sekaran Gunungpati Semarang. Sampel yang diambil sebanyak 20 sampel yang terdiri atas 4 merk air minum kemasan, 8 sampel air minum isi ulang, dan 8 sampel air sumur. Penelitian diawali dengan tahap isolasi E.coli pada medium Eosin Methylen Blue Agar (EMBA), yang dilanjutkan ke medium Sorbitol MacConkey Agar (SMAC) untuk identifikasi E.coli O157 dilanjutkan uji lateks aglutinasi (OXOID) dan diakhiri dengan uji konfirmasi gen rfbE menggunakan teknik Polymerase Chain Reaction (PCR). Hasil penelitian menunjukkan 8 sampel yang positif E.coli pada medium SMAC menunjukkan positif E.coli O157 (colorless). Uji lateks aglutinasi juga menunjukkan 8 sampel positif E.coli O157 seperti kontrol ATCC 43894. E.coli ATCC 43894 dan 8 sampel E.coli dari berbagai air minum di Kelurahan Sekaran Gunungpati Semarang menunjukkan positif E.coli O157.
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Iijima, A., T. Meguro i I. Yamato. "Thermostability of E.coli trprepressor". Seibutsu Butsuri 41, supplement (2001): S159. http://dx.doi.org/10.2142/biophys.41.s159_4.
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Kleanthous, C. "Suicidal proteins in E.coli". Biochemical Society Transactions 29, nr 3 (1.06.2001): A48. http://dx.doi.org/10.1042/bst029a048b.
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Khafizov, Rustem G., Alexander G. Kozlov, Timothy M. Lohman i Yann R. Chemla. "E.coli SSB Under Tension". Biophysical Journal 98, nr 3 (styczeń 2010): 270a. http://dx.doi.org/10.1016/j.bpj.2009.12.1470.
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Wiggins, Paul, Joshua Martin i Jane Kondev. "Chromatin Organization in E.coli". Biophysical Journal 96, nr 3 (luty 2009): 20a. http://dx.doi.org/10.1016/j.bpj.2008.12.1006.
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Wihansah, R. R. S., M. Yusuf, M. Arifin, A. Y. Oktaviana, Rifkhan Rifkhan, J. K. Negara i A. K. Sio. "Pengaruh Pemberian Glukosa yang Berbeda terhadap Adaptasi Escherichia coli pada Cekaman Lingkungan Asam". Jurnal Sain Peternakan Indonesia 13, nr 1 (10.03.2018): 29–35. http://dx.doi.org/10.31186/jspi.id.13.1.29-35.
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Setiap makhluk hidup termasuk bakteri memiliki kemampuan untuk beradaptasi pada kondisi lingkungan yang tidak sesuai dengan zona nyamannya. Penelitian ini bertujuan untuk melihat respon bakteri E.coli terhadap kondisi asam atau pH rendah sebagai bentuk adaptasinya. Pada penelitian ini terdapat 5 perlakuan yaitu E.coli yang ditumbuhkan pada media Nutrien Broth sebagai kontrol (P0), E.coli yang ditumbuhkan pada media Nutrien Broth dengan kondisi asam (pH 4) (P1), E.coli yang ditumbuhkan pada media Nutrien Broth dengan kondisi asam (pH 4) dan diberi glukosa 10 % (P2), selanjutnya diberi glukosa 30 % (P3) dan 50 % (P4). Pada setiap perlakuan diulang sebanyak 3 kali. Jumlah populasi dihitung dengan Metode Turbidimetri menggunakan spektrofotometer melalui nilai kekeruhan (OD). Hasil penelitian ini menunjukkan bahwa media dengan pH rendah (pH 4) dapat menghambat pertumbuhan bakteri E.coli, sedangkan penambahan glukosa mampu mempertahankan pertumbuhan bakteri E.coli. Penambahan glukosa sampai 50 % terbukti mampu meningkatkan populasi sampai jam ke 29, sedangkan pada pemberian glukosa 10 dan 30 % sudah terjadi penurunan populasi bakteri E.coli pada jam ke 29.Kata kunci: E.coli, adaptasi, asam, populasi bakteri
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Сэргэлэн, H., C. Лхагвасүрэн i Д. Алтангэрэл. "“ЭМГЭГ ТӨРӨГЧ E.COLI-ИЙН ХОРУУ ЧАНАРЫН ЗАРИМ СУДАЛГАА”". Mongolian Journal of Agricultural Sciences 12, nr 1 (7.12.2014): 3–7. http://dx.doi.org/10.5564/mjas.v12i1.244.
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Манай оронд өргөн дэлгэр тархсан (ундны ус, хүнс тэжээл, мал, ахуйд. г.м) E.coli бүлгийн нянгийн эмгэг төрүүлэгч хэв шинжийг тодорхойлох, оношлогооны технологийг боловсронгуй болгох нь хүнсний халдвар хордлогоос сэргийлэх арга зам юм. Энэхүү судалгаагаар хүн, мал эмнэлэгийн лабораторийн шинжилгээ, судалгааны ажлын үр дүнгээр олдсон E.coli-ийн 24 өсгөвөрийн 79.1 % (19), E.coli-ийн Mal B генд эерэг, 7 % (5) нь eae генийн өвөрмөц дараалалд эерэг байна. Молекул эпидемиологийн судалгаагаар мах түүнд хамаарагдах гадаад орчноос ялгасан E.coli-ийн 16,7% хоолны хордлоготой өвчтнөөс ялгасан E.coli-ийн 25% нь EHEC байна.DOI: http://dx.doi.org/10.5564/mjas.v12i1.244
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Silverman, Lewis B., Donna Neuberg, Jeffrey Supko, Mary Relling, Christina Woodward i Stephen E. Sallan. "Pharmacodynamics and Tolerability of Twice-Weekly Erwinia Asparaginase after E. coli Asparaginase Allergy in Children with ALL." Blood 108, nr 11 (16.11.2006): 1857. http://dx.doi.org/10.1182/blood.v108.11.1857.1857.
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Abstract BACKGROUND: E.coli L-asparaginase (ASP) is an important component of multiagent chemotherapy for childhood ALL; however, allergic symptoms develop in 25–30% of patients (pts). Erwinia ASP is an alternative preparation which has been used after E.coli ASP allergy; however, it has not been previously shown how well E.coli ASP-allergic pts tolerate Erwinia ASP or whether they achieve therapeutic ASP levels. METHODS: On DFCI ALL Consortium Protocol 00–01 (2000–2005), newly diagnosed children with ALL (aged 1–18 years) received 30 weeks (wks) of IM ASP during consolidation beginning 7 weeks after diagnosis. All pts initially received weekly E.coli ASP. Nadir serum ASP concentrations were measured every 3 wks by a validated biochemical assay and antibodies to E.coli and Erwinia ASP were measured by ELISA every 6 wks. If E.coli ASP allergy developed, children received Erwinia ASP (25000 IU/m2/dose twice weekly) until 2003, when Erwinia ASP became unavailable. RESULTS: We analyzed the data of all 44 patients treated between 2000–2002 who received Erwinia ASP after E.coli ASP-allergy. Median age at diagnosis was 5.5 years. Median duration of E.coli ASP prior to development of allergy was 5 wks (range 1–23). 26 (59%) pts were positive for E.coli ASP antibodies. Erwinia ASP toxicities included: allergy in 15 (34%) pts (which occurred a median 8 wks after starting Erwinia, range 2–15.5), pancreatitis in 1 (2%) and insulin-requiring hyperglycemia in 1 (2%) pt. 18 (41%) pts became positive for Erwinia ASP antibodies, 4 of whom developed Erwinia ASP allergy. Highest nadir ASP concentrations observed after Erwinia ASP in the 44 E.coli-allergic pts are displayed in the Table (below). Prior data has suggested that a nadir serum ASP level of >=0.1 IU/mL is associated with asparagine depletion (therapeutic level). Nadir Serum ASP Levels after Erwinia ASP in E.coli ASP-allergic pts N Median (range) ASP Level (IU/mL) % with ASP Level >= 0.1 IU/mL All E.coli ASP allergic pts 44 0.231(0.00–3.33) 84% Subsequent allergy to Erwinia Yes 15 0.418 (0.00–3.33) 80% No 29 0.215 (0.00–0.93) 86% E.coli ASP antibody positive 26 0.171 (0.00–3.33) 73% negative 18 0.318 (0.10–0.93) 100% Erwinia ASP antibody Positive 18 0.171 (0.00–3.33) 72% negative 26 0.322 (0.00–0.93) 92% Highest ASP concentration with E.coli ASP prior to allergy Subtherapeutic(<0.1 IU/mL) 20 0.221 (0.00–1.04) 85% Therapeutic(>=0.1 IU/mL) 24 0.323 (0.00–3.33) 83% Excluding pts who switched to PEG when Erwinia became unavailable in 2003 (N=11), pts remained on Erwinia ASP for a median of 16 wks (range 2–28). 31 pts (70%) ultimately completed all planned 30 wks of ASP consolidation. CONCLUSIONS: We conclude that twice-weekly Erwinia ASP is well-tolerated and achieves detectable and potentially therapeutic serum ASP levels in the majority of E.coli ASP-allergic pts, including those with ASP antibodies (E.coli and/or Erwinia), pts who developed subsequent allergy to Erwinia ASP and pts who never achieved therapeutic nadir levels with prior E.coli ASP. Erwinia ASP should be considered as alternative therapy for pts with ALL who develop E.coli ASP allergy.
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Hussain, Saman ,., Shaista Alam, Shahina Mumtaz, Ihsan Ullah, Momena Ali i Noor Rehman. "Antibiogram and Frequency of BLA-NDM-1 Gene in E.Coli Isolated from Carbapenem Resistant Cases of UTI Patients". Pakistan Journal of Medical and Health Sciences 16, nr 1 (31.01.2022): 1470–72. http://dx.doi.org/10.53350/pjmhs221611470.
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Background: Urinary tract infections (UTI) are very common in all the population around the world. Women are the usual victims of the super bug Escherichia coli (E.coli) and usually suffer from UTI once in a lifetime. The multi-drug resistant (MDR) E.coli is very intelligent creature and turned resistant to the antibiotics through transposons, mutations and plasmids. Most of the E.coli harbors the gene bla-NDM-1 due to which they have become highly resistant to Carbapenem antibiotics. Objectives: To find the bla-NDM-1 gene in Carbapenem resistant E.coli isolated from UTI patients. To determine the frequency of Carbapenem resistant E.coli in patients attending tertiary care hospital of Peshawar. Methodology: This study was conducted in Khyber Teaching Hospital Peshawar from January 2019 to June 2019. we collected 87 urinary E.coli isolates which were resistant to Carbapenem by disc diffusion method on Mueller Hinton Agar. Polymerase chain reaction (PCR) was done to unyield the presence of bla-NDM-1 gene in these 87 cases. Results: According to this study E.coli was the most common causative agent for UTI and females suffered more than male patients. The antibiotics Tigecycline and Colistin showed 100% sensitivity against E.coli. Some of the antibiotics like Ampicillin; Meropnem etc were 100% resistant to E.coli. The gene bla-NDM-1 turned out to be positive in 26.43% of the cases. Conclusion: This study concluded that all the E.coli isolated from UTI patients having bla-NDM-1 gene exhibit resistance to Carbapenem. These isolates are very difficult to treat and limited therapeutic options are available. Keywords: Multi- drug resistant Escherichia coli, Carbapenemases, Antibiogram, Plasmids, Polymerase chain reaction, Transposons and Mutations.
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Al-Khyat, Fadia Abd AL-Muhsin. "Enterohaemorrhagic E.coli O157 in locally produced soft cheese". Iraqi Journal of Veterinary Medicine 32, nr 1 (30.06.2008): 89–99. http://dx.doi.org/10.30539/iraqijvm.v32i1.770.
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Escherichia coli is consedered to be one of the normal flora of the humanand animal intestinal tract, but there are some serotypes which are diagnosedas a pathogenic such as Enteroheamorrhagic E.coli O157 that cause diseaseranging in severity from mild to lethal and it's significant as a major foodbornepathogen and as a public health problem. The purpose of this studywas to provide the prevalence of E.coli and E.coli O157 in locally producedsoft cheeses, detection and enumeration were carried out using specificdifferential media (CT.SMAC,E.M.B.), serological test (Latex O157) andbiochemical test.A total of 50 samples of locally produced soft cheeses were collected fromsupermarket in Baghdad City during the period from April till the end ofMay 2007. The result revealed that there was a significant (P<0.05)difference in the isolation percentage, E.coli was isolated from 47 samples(94%) while E.coli O157 isolated from 40 samples (80%). The data alsorevealed that there was a significant (P<0.05) difference in the mean ofmicrobial counts E.coli and E.coli O157 recorded (4.7x106 , 2.4x105CFU/g) respectively. These result suggest that the serotype O157 isprevalent within dairy product and processing environment.
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Saad, Susana M. I., i Bernadette D. G. M. Franco. "Influence of raw meat natural background flora on growth of Escherichia coli O157: H7 in ground beef". Revista de Microbiologia 30, nr 3 (lipiec 1999): 272–77. http://dx.doi.org/10.1590/s0001-37141999000300015.
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Escherichia coli O157:H7 is a foodborne pathogen of increasing importance. It has been involved in several threatening outbreaks, most of them associated with meat products. In this study, the influence of some bacteria from the natural background flora of raw meat over E.coli O157:H7 in ground beef stored under refrigeration and at room temperature was evaluated. Different levels of E.coli O157:H7 (101-102, 103-104 and 106-107 CFU/g), inoculated in ground beef samples, were challenged with strains of non-pathogenic E.coli, Pseudomonas putida or Leuconostoc sp. Growth of the pathogen was monitored using standard cultural methods and an ELISA-type rapid method. Non-pathogenic E.coli, Pseudomonas putida and Leuconostoc sp. did not affect growth of E.coli O157:H7 in ground beef, both under refrigeration and at room temperature. Based on these findings, the low occurrence of E.coli O157:H7 in raw meat may not be attributed to antagonistic effects of bacteria from the natural background flora.
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Abdullah, S. M., Sahar H. Abdulmaged i S. M. Hamad. "COMPARATIVE STUDY BETWEEN PHYTOGENIC AND ESCHERICHIA COLI VACCINE IN BROILERS". IRAQI JOURNAL OF AGRICULTURAL SCIENCES 53, nr 1 (23.02.2022): 157–63. http://dx.doi.org/10.36103/ijas.v53i1.1520.
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The present study carried out to comparative between usage of killed vaccine of Escherichia coli with aromatic oils or alone against infection of broiler with pathogenic E.coli for this purpose two hundred broiler chicks were collected and randomly divided into four groups. Each group contained fifty chicks, food and drink for all groups ad libitium. The experimental groups were treated as follows: G1 vaccinated with the killed E. coli vaccine through subcutaneous injection dose 0.5 ml (3×107 CFU/ml )in day five old and received Digestarom® P.E.P sol. in drinking water 6 ml/100L from one day old until end of trial. G2 vaccinated with killed E. coli only through subcutaneous injection dose 0.5 ml (3×107 CFU/ml) in day five old. G3 received Digestarom® P.E.P sol. in drinking water 6 ml/100Lonly one day old until end of trial. G4 control negative. The experimental birds in day 25 were challenged intratracheally inculated with Escherichia coli of 0.5 ml was LD50(3 ×108 CFU/ml) in the experiment. Blood samples were taken at day 20 and 35 for assessment of different parameters, mortality and lesions score were determined . The results showed that the use of aromatic oils and received killed vaccine of E.coli has a good effect on the immune response , decreasing the mortality rate and lesion score of E. coli infected chickens. It can be concluded from the current study that the use digestarom enhancement the immune response after vaccination with E.coli vaccine against pathogenic E.coliIt also the use E.coli vaccine with the digestarom in broiler chickens is more effective than using the vaccine alone
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Qurrata'yuni Pratiwi, Eustachius Hagni Wardoyo i Eva Triani. "UJI AKTIVITAS ANTI KOLONISASI BAKTERI ASAM LAKTAT DARI KEFIR SUSU KAMBING TERHADAP BAKTERI Escherichia coli PENGHASIL Extended Spectrum Beta Lactamase PADA USUS MENCIT BALB/c". Unram Medical Journal 10, nr 4 (22.01.2022): 613–19. http://dx.doi.org/10.29303/jku.v10i4.634.
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Escherichia coli (E.coli) adalah salah satu jenis spesies bakteri Gram negatif yang merupakan flora normal pada saluran pencernaan. Namun, berpotensi dapat menjadi patogen apabila jumlahnya dalam saluran pencernaan meningkat. Terinfeksi E.coli dapat menimbulkan beragam penyakit radang usus seperti IBD, Crohn dan kolitis ulserativA. Beberapa studi menemukan bahwa probiotik dapat berperan sebagai agen pencegahan dan terapeutik untuk mengeradikasi bakteri patogen melalui penghambatan adhesi dan kolonisasi. Penelitian ini dilakukan bertujuan untuk mengetahui potensi BAL dalam menghambat kolonisasi E.coli penghasil ESBL secara in-vivo pada mencit BALB/c. Hasil penelitian uji kolonisasi secara in vivo menunjukkan bahwa BAL memiliki aktivitas penghambatan kolonisasi yang baik terhadap E.coli penghasil ESBL yang ditunjukkan melalui penurunan jumlah koloni E.coli penghasil ESBL pada mencit BALB/c yang di induksi pelet dan CFS BAL.
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Lenehan, Dearbhla, Alyson Murray, Stephen Smith, Bernt Eric Uhlin i Jennifer Mitchell. "Characterisation of E. coli Lipopolysaccharide Adherence to Platelet Receptors". Blood 128, nr 22 (2.12.2016): 4906. http://dx.doi.org/10.1182/blood.v128.22.4906.4906.
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Abstract Introduction The interaction of bacteria with platelets is vital in the pathogenesis of sepsis and other fatal infections. Sepsis is an inflammatory response to bloodstream infections with high mortality rates and Escherichia coli (E.coli) is the most frequently isolated gram-negative organism in these infections. Platelets are a part of our innate immune system and interact with invading pathogens in our blood stream. Recently, it has been shown that E.coli interacts directly with platelets and it is believed that Lipopolysaccharide (LPS) plays a role in this interaction. Understanding this adhesive process and identification of the platelet receptors involved in bacterial adherence is vital in explaining the underlying mechanisms utilized in infections. Methods Competition assays assessing E.coli adherence to platelets in the presence of antibodies to platelet surface receptors and soluble recombinant forms of these receptors were conducted. Competition assays were used to assess E.coli binding to platelets in the presence of E.coli K12 LPS and H.pylori LPS. Differences between the affinity of human and murine receptors to E.coli were investigated by assessing E.coli binding to recombinant human and murine CD36. E.coli strains expressing LPS structural variations were assessed to identify the LPS moiety that promotes binding to platelets and CD36. Results This study demonstrates for the first time that the scavenger receptor CD36 and the Toll-like receptors; TLR2 and TLR4/MD2 are platelet receptors for the direct adherence of E.coli LPS to platelets. LPS is a large molecule found on the outer membrane of gram-negative bacteria that elicits a strong immune response in humans and animals. It comprises of three main components; the O-antigen, a core polysaccharide and the lipid A region. Our work reveals that the minimum LPS components required for E.coli binding to platelets and CD36 must contain the Lipid A and inner core regions of LPS. Identifying this interaction to host receptors is important if we want to design effective anti-sepsis drugs. In addition to this, analysis of the adherence of epidemic strains of pathogenic antibiotic resistant strains of E.coli to platelets and recombinant platelet receptors showed that the O-antigen portion of LPS may conceal LPS from recognition by these receptors as these strains bind significantly less to CD36 compared to an E. coli strain that does not express an O-antigen. This information helps explain how these pathogenic E.coli strains can evade immune recognition and therefore, continue to infect patients. Interestingly, murine CD36 has a significantly reduced affinity for E.coli compared to human CD36. This may help to explain why the human and mouse immune response to E.coli infection is different and perhaps explain the failure of anti-sepsis drugs developed in mouse models of infection like Eritoran in human clinical trials. This evidence suggests that mouse models may not be optimal models for E. coli septicemia. Conclusion This research provides a new insight into the mechanisms of direct E.coli platelet interactions that can cause life-threatening diseases. We have identified the LPS components involved in the direct interaction of E.coli with platelets and that pathogenic strains can utilize the O-antigen region of their LPS structure to evade immune recognition so they can continue to infect patients. Understanding how these strains interact with our immune cells or evade them will be invaluable in designing effective treatments to combat infections. Disclosures No relevant conflicts of interest to declare.
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Alwan, Ali Hussein, i Noor Naeem Khwen. "DetectionOf genesResponsible for BiofilmsFormedby Klebsiella pneumoniaeandEsherichia coliand their effect on innate immunity". Al Mustansiriyah Journal of Pharmaceutical Sciences 17, nr 1 (13.03.2018): 12. http://dx.doi.org/10.32947/ajps.v17i1.75.
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Biofilms as a major virulence factor of bacteria ,therelationship between bacteria persistence in the urinary tract and genes responsible for biofilms production was studied. The aim is to detect the presence,frequency of the,fimA, fimH, mrkA and mrkD, genes and biofilms production in two species isolated from UTI patients and their effect on some innate immunity aspects. Sixty five isolates of E.coli and fifty of K.pneumoniae isolates were collected. All isolates were initially diagnosed as genus Esherichiacoli andKlebsiella pneumonia. Final identification for the isolates was done by Vitek 2 compact system. The ability to form biofilm was carried out by using Tissue culture plate method (TCP).Adhesion average to epithelial cells of E.coli and K.pneumoniae was studied. Detection of genes were done to by using PCR technique . Immunological experiments were done by using bactericidal activity and opsonization factor. UTI infection were more common in female than male. 75% (49/65) females, 25%(16/65) males ofE.coli isolates and 88% (44/50) females,12%(6/50) males of K.pneumoniaeisolates. Patients ages in children between (2 months -18years) in UTI caused by E.coli was 35%(23/65) while in k.pneumoniae was 74%(37/50),Ages between (18 years-50 years) in E.coli was 37%(24/65) while in K.pneumoniae was 18%(9/50) finally,Ages between (50 years-72years) in E.coli was 28%(18/65) while in K.pneumoniae was 8%(4/50).69% of E.coli isolates and 72% of k.pneumoniae isolates were higher biofilm producers. . Adhesion average to epithial cells of E.coli and K.pneumoniae was 42.9% and 44% respectively. Both E.coli and K.pneumoniae showed that 100% 0f the 12 isolate harbored the fimA, fimH, mrkA and mrkD genes. The bactericidal average was higher in E.coli than K.pneumoniae , P- value( 0.01) for mice injected with fimbriae, Opsonization factor was higher in E.coli than K.pneumoniae the P value (0.05) . Conclusion: A strong relationship between biofilms production and presence of genes of different species isolated from same clinical source that has effected on resistance of bacteria to innate immunity.
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Trisdayanti, Ni Putu Eka, Anak Agung Sagung Sawitri i I. Nengah Sujaya. "Higiene Sanitasi dan Potensi Keberadaan Gen Virulensi E.Coli pada Lawar di Kuta: Tantangan Pariwisata dan Kesehatan Pangan di Bali". Public Health and Preventive Medicine Archive 3, nr 2 (1.12.2015): 99. http://dx.doi.org/10.15562/phpma.v3i2.99.
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Latar belakang dan tujuan: Kejadian luar biasa (outbreak) terkait makanan telah berulangkali terjadi pada wisatawan maupun penduduk setempat dan mungkin terkait dengan keberadaan E.coli pathogen. Karena lawar (makanan tradisional Bali) digemari penduduk setempat dan wisatawan, diperlukan studi untuk mengetahui keberadaan E.coli patogen pada lawar dan kaitannya dengan higiene sanitasi warung makan di Kuta.Metode: Penelitian crossectional analitik dilakukan pada 43 warung yang menjual lawar putih di Kuta Utara, Kuta, dan Kuta Selatan beserta pengolahnya. Observasi dan wawancara dilakukan untuk menilai penerapan higiene perperorangan, sanitasi warung, sanitasi peralatan dan fasilitas sanitasi. Sampel lawar putih diambil dari setiap warung dan diuji di laboratorium. Indikator kualitas makanan yang diuji adalah jumlah koloni bakteri dengan Total Plate Count (TPC), keberadaan E.coli dengan teknik pemupukan dan deteksi gen virulen E.coli dengan teknik PCR. Data dianalisis secara univariat, bivariat dan multivariat.Hasil: Sebanyak 44,2% lawar memiliki jumlah koloni bakteri >106 CFU/gr; 46,5% lawar positif E.coli, dan 20% dari sampel yang positif E.coli menunjukkan gambaran band dengan ukuran mirip Shiga Like Toxin Type I (SLT-I). Lawar yang terkontaminasi lebih banyak di wilayah Kuta Utara. Higiene penjual yang kurang baik lebih berisiko terhadap keberadaan E.coli dibandingkan higiene penjual yang baik (adjusted OR 7,29; 95%CI: 1,473-36,088).Simpulan: Kualitas lawar di Kuta masih kurang baik dan disertai penerapan higiene sanitasi yang rendah
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Kleinheinz, Gregory T., Tabitha Zehms i Kathryn Koenig. "Evaluation of Stormwater Biofilter Media for Escherichia coli Removal in a Laboratory Microcosm". Air, Soil and Water Research 1 (styczeń 2008): ASWR.S825. http://dx.doi.org/10.4137/aswr.s825.
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Recreational water sites such as beaches along lakes, rivers, or oceans, are one of the most popular activities in many parts of the world. Recently rainfall and runoff due to rainfall events has been associated with increasing microbial levels in recreational water. This runoff can lead to beach closures and potentially unsanitary conditions at popular swimming beaches. The impact of stormwater on beach water quality has led to a myriad of option for controlling stormwater. Some of these include grass buffer partitions, stormwater detention basins, media filters, catch basin inserts, and infiltration units. Biofilters, or infiltration units are gaining popularity as a treatment option for stormwater around the Great Lakes basin, but we are aware of no studies that have looked at the indicator organism (i.e. Escherichia coli, or E.coli) removal potential of these infiltration units and the media used in them. The overall objective of this study was to evaluate the performance of a stormwater biofilter medium in removing the indicator organism E.coli in a laboratory system. When several laboratory biofilter system were challenged with E.coli concentrations of 2.82E3 and 2.85E5 E.coli/100mL of simulated stormwater in a 1.25 cm rain event, the systems were able to remove between 83 and 100% of the E.coli in this influent. During a subsequent 1.25 cm rain event with E.coli-free water, the biofilter was able to retain 68%-100% of the E.coli originally inoculated into the system. The results of this study indicate that these systems hold promise for mitigation of E.coli from storm water near recreational beaches. These findings will assist beach managers, engineers, and municipal stake holders evaluate the usefulness of biofilter infiltration as a storm water management tool in order to decrease E.coli input into beach areas.
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Najim, Najm Hadi, i Amer Jebur Obayes AL-Isawi. "Effect power levels in microwave of E.Coli O 157:H7 from bovine milk and Soft Cheese samples in Babylon Province". Kufa Journal For Veterinary Medical Sciences 8, nr 1 (30.06.2017): 208–17. http://dx.doi.org/10.36326/kjvs/2017/v8i14297.
Pełny tekst źródłaStreszczenie:
Colonies of E.Coli O157:H7 were isolated from 80 locally produced Cow's and Buffalo's soft cheese samples that were collected randomly at weekly intervals (5 samples/week) from various retail markets in different locations of babylon province during two climatic periods(40 samples /species/season) where the first periad was extended from the begining of December 2015 to the end of February 2016 while the second period was extended from the begining of July to the end of September 2016.In addition,50 fresh cattle faecal samples were collected from different farms in babylon province for the isolation of E.Coli O157:H7.The identification of E.Coli O157:H7 isolates were confirmed based on their cultural, biochemical and seralogical characteristics using the commercial latex agglutination test kit .Data revealed that there was a significant (P≤ 0.05) seasonal variation in the prevalence of E.Coli O157:H7 where all Cow's and Buffalo's soft cheese samples had significantly (P≤ 0.05) higher prevalence of E.Coli O157:H7 in summer season (50% and 40% respectively) than in winter season (25% and 15% respectively).It was found that all of the 80 bovine soft cheese samples had significantly (p≤0.05) higher prevalence of E.Coli O157:H7 in summer (45%) than in winter (20%) seasons.In addition to that, all the 50 bovine faecal samples had significantly (P≤ 0.05) higher prevalence of E.Coli O157:H7 in summer (72%) than in winter (40%) seasons. Complete elimination of viable E.Coli O157:H7 was achieved when the inoculated milk was subjected to the microwave power level of 900 watts after 30 seconds of exposure. Complete elimination of viable E.Coli O157:H7 was acheived when the contaminated soft cheese samples were subjected to the microwave power level of either 300 or 450 watts for 60 seconds of exposure.
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Najim, Najm Hadi, i Amer Jebur Obayes AL-Isawi. "Effect power levels in microwave of E.Coli O 157:H7 from bovine milk and Soft Cheese samples in Babylon Province". Kufa Journal For Veterinary Medical Sciences 8, nr 1 (30.06.2017): 208–17. http://dx.doi.org/10.36326/kjvs/2017/v8i14297.
Pełny tekst źródłaStreszczenie:
Colonies of E.Coli O157:H7 were isolated from 80 locally produced Cow's and Buffalo's soft cheese samples that were collected randomly at weekly intervals (5 samples/week) from various retail markets in different locations of babylon province during two climatic periods(40 samples /species/season) where the first periad was extended from the begining of December 2015 to the end of February 2016 while the second period was extended from the begining of July to the end of September 2016.In addition,50 fresh cattle faecal samples were collected from different farms in babylon province for the isolation of E.Coli O157:H7.The identification of E.Coli O157:H7 isolates were confirmed based on their cultural, biochemical and seralogical characteristics using the commercial latex agglutination test kit .Data revealed that there was a significant (P≤ 0.05) seasonal variation in the prevalence of E.Coli O157:H7 where all Cow's and Buffalo's soft cheese samples had significantly (P≤ 0.05) higher prevalence of E.Coli O157:H7 in summer season (50% and 40% respectively) than in winter season (25% and 15% respectively).It was found that all of the 80 bovine soft cheese samples had significantly (p≤0.05) higher prevalence of E.Coli O157:H7 in summer (45%) than in winter (20%) seasons.In addition to that, all the 50 bovine faecal samples had significantly (P≤ 0.05) higher prevalence of E.Coli O157:H7 in summer (72%) than in winter (40%) seasons. Complete elimination of viable E.Coli O157:H7 was achieved when the inoculated milk was subjected to the microwave power level of 900 watts after 30 seconds of exposure. Complete elimination of viable E.Coli O157:H7 was acheived when the contaminated soft cheese samples were subjected to the microwave power level of either 300 or 450 watts for 60 seconds of exposure.
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Gu, Shao Bin, Ke Wei Li, Ying Wu, Shi Chang Li i Guo Wei Lu. "Potential Role of Calcium Lon in Mediating the Response of Escherichia Coli to Audible Sound Stimulation". Advanced Materials Research 343-344 (wrzesień 2011): 1079–86. http://dx.doi.org/10.4028/www.scientific.net/amr.343-344.1079.
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Previous studies from our laboratory have indicated that audible sound field stimulation can significantly affect E.coli growth and metabolic action, which can enhance or inhibit the growth of Escherichia coli (E.coli), and the effects of sound field on E.coli growth depended greatly on the intensity and frequency of sound field. However, very little efforts have been put forth in studying the potential mechanism of bacterial cells responses to audible sound stimulation. In this paper, the potential role of calcium signaling in mediating the response of E.coli to audible sound stimulation was firstly reported. We found that audible sound wave stimulation could evidently enhance total intracellular Ca2+ content. And the lack of calcium ion in medium significantly alleviated audible sound wave biological effects. Moreover, by adding appropriate level of calcium chloride to the LB medium contained 1.2 mM EDTA, the promotion effect of audible sound wave to E.coli growth was gradually resumed. On the basis of these findings, we speculate that calcium signaling may play an important role in mediating the response of E.coli to audible sound stimulation.
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Azizah, Nur, Abdur Rivai i Rasman. "Faktor Yang Berhubungan Dengan Keberadaan Bakteri Escherichia Coli Pada Air Sumur Gali Di Kelurahan Jeppe’e Kec.Tanete Riattang Barat Kab.Bone". Sulolipu: Media Komunikasi Sivitas Akademika dan Masyarakat 23, nr 2 (18.12.2023): 207–15. http://dx.doi.org/10.32382/sulo.v23i2.71.
Pełny tekst źródłaStreszczenie:
Air sumur gali mengalami penurunan kualitas dari segi bakteriologis. Air sumur masyarakat mengandung bakteri E.Coli yang dapat menyebabkan penyakit diare. Hal tersebut dipengaruhi oleh faktor jarak sumber pencemar, perilaku pemilik sumur gali dan konstruksi sumur gali yang tidak memenuhi syarat. Penelitian ini bertujuan untuk mengetahui faktor yang berhubungan dengan keberadaan bakteri E.Coli pada air sumur gali di Kelurahan Jeppe’e Kec. Tanete Riattang Barat Kab. Bone. Penelitian ini adalah observasional analitik dengan rancangan penelitian Cross Sectional, teknik sampling yang digunakan non probality (purposive sampling) jumlah sampel 9 dan dianalisis menggunakan uji exact fisher. Hasil penelitian menunjukkan ada hubungan antara jarak sumber pencemar (septictank, SPAL dan kandang ternak) dengan keberadaan bakteri E.Coli pada air sumur gali dengan nilai ρ= 0,048 < α= 0,05, ada hubungan konstruksi sumur gali (bibir, dinding dan lantai sumur gali dan saluran pembuangan air limbah) dengan keberadaan bakteri E.Coli pada air sumur gali dengan nilai ρ= 0,008 < α= 0,05, dan tidak ada hubungan perilaku pemilik sumur gali (peletakan timba, mandi dan mencuci di area sumur) dengan keberadaan bakteri E.Coli pada air sumur gali dengan nilai ρ= 1,000 ˃ α= 0,05.Terdapat hubungan jarak sumber pencemar dan konstruksi sumur gali dengan keberadaan bakteri E.Coli pada air sumur gali dan tidak terdapat hubungan antara perilaku pemilik sumur gali dengan keberadaan bakteri E.Coli pada air sumur gali. Diharap kepada masyarakat agar dalam pembuatan sumur gali memperhatikan jarak sumber pencemar (septictank, SPAL, kandang ternak) dan konstruksi sumur gali (bibir sumur, dinding sumur, lantai sumur dan saluran pembuangan air limbah) agar terhindar dari cemaran bakteri E.Coli. Kata kunci : Air Sumur Gali, Sumur Gali, Jarak, Konstruksi
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Febrianti, Tati, Sundari Nursofiah, Novi Amalia, Dwi Febriyana, Ratih Dian Saraswati, Nelly Puspandari, Sunarno Sunarno i Efadeswarni Efadeswarni. "Performa Tryptone Bile X-Glucuronide (TBX) yang disuplementasikan dengan Cefotaxime sebagai Medium Selektif Untuk Skrining ESBL-E.coli dari Sampel Lingkungan". Buletin Penelitian Kesehatan 49, nr 1 (5.04.2021): 1–8. http://dx.doi.org/10.22435/bpk.v49i1.3858.
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Identification of ESBL-E.coli from environment without selective medium will be challenging to do considering that E.coli mixes with various other microorganisms in the environment. This study aimed to determine the performance of TBX Agar supplemented with Cefotaxime as a selective medium for ESBL-E. coli screening from 138 water samples of environmental sampling obtained from rivers, open sewers in the market, poultry slaughterhouses and hospital waste water inlets and outlets around Jakarta. Laboratory examinations were carried out through the filtration stage, inoculation on the TBX Agar supplemented with Cefotaxime medium as well as species confirmation and ESBL with the indol test and double-disk test. The results showed that 87.08% (40-100%) of suspect colonies growing on TBX Agar supplemented with Cefotaxime medium were confirmed as E.coli and 82.51% (12-100%) were confirmed as ESBL-E.coli. However, there was no correlation between TBX Agar supplemented with Cefotaxime performance and sampling locations. Based on the results of the study, it can be concluded that the TBX supplemented with Cefotaxime medium can be used for ESBL-E.coli screening in the environment, but further confirmation is needed using the indole and double-disk tests.
Keywords: Escherichia coli, ESBL, TBX Agar suplemented with Cefotaxime
Abstrak Identifikasi ESBL-E.coli tanpa medium selektif akan sangat sulit dilakukan pada sampel lingkungan mengingat ESBL-E.coli bercampur dengan berbagai mikroorganisme lainnya. Tujuan penelitian ini yaitu untuk mengetahui performa TBX Agar yang disuplementasi Cefotaxime sebagai medium selektif untuk skrining ESBL-E.coli. Sampel penelitian sebanyak 138 sampel air lingkungan yang diambil dari sungai, saluran pembuangan terbuka di pasar, rumah pemotongan hewan unggas (RPHU) serta inlet dan outlet limbah rumah sakit di sekitar Jakarta. Pemeriksaan laboratorium melalui tahapan filtrasi, inokulasi pada medium TBX Agar dengan suplementasi Cefotaxime serta konfirmasi spesies dan ESBL dengan uji indol dan double-disk test. Hasil menunjukkan bahwa koloni tersangka yang tumbuh pada medium TBX Agar dengan suplementasi Cefotaxime sebanyak 87,08% (40-100%) terkonfirmasi sebagai E.coli dan 82,51% (12-100%) terkonfirmasi sebagai ESBL-E.coli. Namun, tidak ada hubungan antara performa TBX Agar dengan suplementasi Cefotaxime dengan lokasi pengambilan sampel. Berdasarkan hasil penelitian dapat disimpulkan bahwa medium TBX Agar dengan suplementasi Cefotaxime cocok digunakan untuk skrining ESBL-E.coli di lingkungan namun tetap diperlukan konfirmasi lanjut menggunakan uji indol dan double disk test.
Kata kunci: Escherichia coli, ESBL, TBX Agar suplemented with Cefotaxime
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Najim, Najim Hadi. "The synergistic bactericidal effects of bacteriocin and pressurization against E.coli O157:H7 in raw milk". Iraqi Journal of Veterinary Medicine 38, nr 1 (1.06.2014): 15–23. http://dx.doi.org/10.30539/iraqijvm.v38i1.249.
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Colonies of E.coli O157:H7 were isolated from 35 raw milk sample and their identification were confirmed based on biochemical reactions and both cultural and serological characteristics. Presumptive E.coli O157:H7 isolates obtained by selective plating on both CT-SMAC and Chromogenic agars were further tested serologically for the presence of both O157 and H7 antigenes using the commercial available latex agglutination test kit. The unhygienic practices in the production of milk in Al-Thahab Al- Abiedh , Abu-Graib, Al-Zedan and Khan Dharie were reflected on the highest significant (p<0.01)prevalence level of contamination with E.coli O157:H7 that appeared to be 80%, 80%, 60% and60% respectively. Homogenization pressure of 1000 psi and 2000 psi for five passes had significantly (p<0.05) influenced the inactivation degree of E.coli O157:H7 in both whole milk and nutrient broth. Milk homogenized at a pressure level of 3000 psi for three passes and 4000 psi for two passes resulted in a further increase of the antimicrobial effectiveness and produced an additional significant (p<0.05) reduction of E.coli O157:H7. Complete elimination (inactivation) of viable E.coli O157:H7 was achieved when cultured whole milk was homogenized at pressure level of 5000 psi for a single pass. Agar well diffusion bioassay was used for the evaluation of antimicrobial activity of the crude bacteriocin produced by L.acidophilus LA-K against E.coli O157:H7. Enterohaemorrhagic E.coli O157:H7 expressed its resistance to the crude bacteriocin since it did not show any inhibition zone around each well treated with bacteriocin. The average diameters of the inhibition zones of crude bacteriocin against stressed E.coli O157:H7 by pressurization at 4000 psi, 3000 psi, 2000 psi and 1000 psi were 14 mm, 12mm, 10mm and 8mm respectively. The homogenization pressure level (moderate or high) had significantly (p<0.05) influenced the inactivation degree of the crude bacteriocin against the stressed E.coli O157:H7 by pressurization. Quantitative measurement of crude bacteriocin antimicrobial activity was determined by using photometric or turbidometric method. The results revealed that no growth of stressed E.coli O157:H7 with no visible turbidity in the nutrient broth with bacteriocin that diluted to 1/2, 1/4 and 1/8 were observed. Bacteriocin that diluted to 1/8 which resulted in no visible turbidity after overnight of incubation at 37C° and gave an optical density reading of 1.448.
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Nagy, Katalin, Zita Kovács, Pál Salamon, Csongor-Kálmán Orbán, Szabolcs Lányi i Beáta Albert. "Enhanced heterologous expression in E.coli". Studia Universitatis Babeș-Bolyai Chemia 64, nr 2 T1 (30.06.2019): 101–10. http://dx.doi.org/10.24193/subbchem.2019.2.09.
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Harley, Calvin B., i Robert P. Reynolds. "Analysis of E.Coli Pormoter sequences". Nucleic Acids Research 15, nr 5 (1987): 2343–61. http://dx.doi.org/10.1093/nar/15.5.2343.
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Alazawi, Yasmeen reyad, i Ibtisam Habeeb Al-Azawi. "Detection and Characterization of hly,cdtI and stxI genes, in MDR uropathogenic and intestinal E.coli isolates, Hilla city, Iraq." AL-QADISIYAH MEDICAL JOURNAL 19, nr 1 (30.06.2023): 24–30. http://dx.doi.org/10.28922/qmj.2023.19.1.24-30.
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Background:. Escherichia coli infections represent a considerable burden in human. Illness caused by E. coli have a significant burden on patients and the healthcare system, so prompt recognition, and appropriate treatment are necessary. Escherichia coli is the first bacterial pathogen in humans responsible for deaths associated with antibiotic resistance
Aim of study: detection of hly, cdtI and stxI genes through molecular analysis in MDR E.coli isolates.
Methods: From November 2021 to May 2022, atotal of 150 urine samples were collected from patients suffering from urinary tract infections were admitted and visit Maternity and Children Babylon Hospital in Al-Hilla city. 100 stool samples were (as control). The identification and antibiotic susceptibility profile of E.coli were done by vitek2 compact system.The isolates were subjected to PCR assays with specific primers for hly , cdtI and stxI .
Results: Only 50(45.5%) E.coli isolates were recovered from the urine samples. In contrast, 60(60%) E.coli isolates from the stool samples. A high rate of MDR was detected among E. coli isolates, where it was( 60%) and (53.3%) in the uropathogenic and intestinal isolates, respectively.
The PCR results were showed that MDR uropathogenic E. coli and intestinal E.coli were hardbourd hly gene (46.7%)and (90.6%), respectively. The cdtI gene was detected in (43.4%) of uropathogenic E. coli, and (40.6%) of intestinal E.coli. The stx gene was detected in (16.7%) of uropathogenic E. coli, and (43.7%) of intestinal E.coli.
Conclusion: The presence of a high percentage of toxin in intestinal E.coli is a serious warning because of the possibility of their becoming virulent if the bacteria change their natural habitat or are exposed to a certain stress.
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Budiyanto, Eko, Nugroho Hari Purnomo, Muzayanah Muzayanah i Aida Kurniawati. "Contamination of E.coli Bacteria in Spamdus Genjahan Water Distribution Network from Karst Groundwater Source". Geosfera Indonesia 9, nr 1 (7.04.2024): 67. http://dx.doi.org/10.19184/geosi.v9i1.37040.
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Karst groundwater is raw water used by Spamdus Genjahan to fulfill domestic water needs. Considering the vulnerability of karst groundwater to pollution and its distribution process, the research aimed to describe the suitability of water in the network as well as the spatial distribution of E.coli and the relationship between distance and it’s concentration. The concentration of E.coli was tested from water samples taken from 20 outlets as representatives of the close, medium and farthest distances from the source. The feasibility of water is carried out by comparing the results of laboratory tests with the water quality threshold value for clean water and domestic purposes. Spatial distribution was analyzed through spatial analysis using Geographic Information Systems, while the relationship between distance and E.coli concentration was carried out using the scatterplot method in GIS. The results showed that the concentration of E.coli in most of the samples was above the allowable threshold for sanitation hygiene purposes. Meanwhile, the distance from the outlet to the source did not show a strong correlation with the level of E.coli concentration. However, there are weak indications that lead to an increase in concentration with the E.coli.
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Najim, Najm Hadi, i Amer Jebur Obayes AL-Isawi. "Molecular detection of Stx1 and Stx2 genes in E.Coli O157:H7 isolated from Cow's and Buffalo's soft cheese samples inBabylon Province, using Multiplex PCR technique." Kufa Journal For Veterinary Medical Sciences 8, nr 1 (30.06.2017): 199–207. http://dx.doi.org/10.36326/kjvs/2017/v8i14302.
Pełny tekst źródłaStreszczenie:
Colonies of E.Coli O157:H7 were isolated from 80 locally produced Cow's and Buffalo's soft cheese samples that were collected randomly at weekly intervals (5 samples/ week) from various retail markets in different locations of babylon province during two climatic periods(40 samples /species/season) where the first periad was extended from the begining of December 2015 to the end of February 2016 while the second period was extended from the begining of July to the end of September 2016.The identification of E.Coli O157:H7 isolates were confirmed based on their cultural, biochemical and seralogical characteristics using the commercial latex agglutination test kit and by duplex PCR technique. Data revealed that there was a significant (P≤ 0.05) seasonal variation in the prevalence of E.Coli O157:H7 where all Cow's and Buffalo's soft cheese samples had significantly (P≤ 0.05) higher prevalence of E.Coli O157:H7 in summer season (50% and 40% respectively) than in winter season (25% and 15% respectively).It was found that all of the 80 bovine soft cheese samples had significantly (p<0.05) higher prevalence of E.Coli O157:H7 in summer (45%) than in winter (20%) seasons.The results of the diagnosis of E.coli O157:H7 were confirmed by PCR assay which showed that,six culturing isolates serotype E.coli O157:H7 were postive by PCR assay and five of these isolates expressed gene of Stx1 whearse one isolate expressed gene of Stx2 .
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Najim, Najm Hadi, i Amer Jebur Obayes AL-Isawi. "Molecular detection of Stx1 and Stx2 genes in E.Coli O157:H7 isolated from Cow's and Buffalo's soft cheese samples inBabylon Province, using Multiplex PCR technique." Kufa Journal For Veterinary Medical Sciences 8, nr 1 (30.06.2017): 199–207. http://dx.doi.org/10.36326/kjvs/2017/v8i14302.
Pełny tekst źródłaStreszczenie:
Colonies of E.Coli O157:H7 were isolated from 80 locally produced Cow's and Buffalo's soft cheese samples that were collected randomly at weekly intervals (5 samples/ week) from various retail markets in different locations of babylon province during two climatic periods(40 samples /species/season) where the first periad was extended from the begining of December 2015 to the end of February 2016 while the second period was extended from the begining of July to the end of September 2016.The identification of E.Coli O157:H7 isolates were confirmed based on their cultural, biochemical and seralogical characteristics using the commercial latex agglutination test kit and by duplex PCR technique. Data revealed that there was a significant (P≤ 0.05) seasonal variation in the prevalence of E.Coli O157:H7 where all Cow's and Buffalo's soft cheese samples had significantly (P≤ 0.05) higher prevalence of E.Coli O157:H7 in summer season (50% and 40% respectively) than in winter season (25% and 15% respectively).It was found that all of the 80 bovine soft cheese samples had significantly (p<0.05) higher prevalence of E.Coli O157:H7 in summer (45%) than in winter (20%) seasons.The results of the diagnosis of E.coli O157:H7 were confirmed by PCR assay which showed that,six culturing isolates serotype E.coli O157:H7 were postive by PCR assay and five of these isolates expressed gene of Stx1 whearse one isolate expressed gene of Stx2 .
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Trisdayanti, Ni Putu Eka, Anak Agung Sagung Sawitri i I. Nengah Sujaya. "Hygiene, Sanitation and Potential Existence of Virulent Genes of E.coli in Lawar in Kuta: The Challenge for Tourism and Safe Food Provision in Bali". Public Health and Preventive Medicine Archive 3, nr 2 (1.12.2015): 99–105. http://dx.doi.org/10.53638/phpma.2015.v3.i2.p03.
Pełny tekst źródłaStreszczenie:
Background and purpose: The reoccurrence of food-borne parasitic outbreaks may be related to contamination by pathogenic strains of E.coli. As lawar (Balinese food) is a favorite of locals and tourists, there needs to be an examination into the presence of pathogenic strains of E.coli in lawar and its relation to hygiene and sanitation standards at food stalls in Kuta. Methods: Cross-sectional analytical study was conducted on 43 stalls that selling white lawar in North Kuta, Kuta and South Kuta. Observations and interviews to sellers were conducted to assess the personal hygiene practices, equipment/stall sanitation and sanitary facilities. Lawar samples were tested in the laboratory. Indicators of the food quality was the number of bacterial colonies with Total Plate Count (TPC), the presence of E.coli with fertilization techniques and the detection of E.coli virulence genes by PCR. Data were analyzed using univariate, bivariate and multivariate analysis. Results: As many as 44.2% of lawar contained bacterial colony >106 CFU/g; 46.5% of lawar positively contained E.coli, and 20% of those positive E.coli had the similar size band of Shiga Like Toxin Type I (SLT-I). The sellers with poor hygiene had higher risks of the presence of E.coli in the lawar compared with those having higher hygiene standards (adjusted OR=7.29; 95% CI 1.473-36.088). Conclusion: Lawar quality in Kuta was poor, as were hygiene practices and seller/stall sanitation.
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Assim Hameed, Roaa. "Effectiveness of Phenol Compound Isolated from Menthe spicata Leaves and Nano – Zinc Oxide on Antimicrobial Activity". Al-Kufa University Journal for Biology 15, nr 1 (27.03.2023): 18–26. http://dx.doi.org/10.36320/ajb/v15.i1.11723.
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The aim from this the study effect Phenol compound isolated from Menthe spicata leaves and Nano – Zinc Oxide on Antimicrobial activity antifungal (Candida albicans ) and antibacterial (staphylococcuse aureas and Escherichia coli) , The Menth spicata plant collection from the city of Karbala in 2/9/ 2022 after cleaning and removing soil. As the seed were washed three times with tap water , extraction by using soxhelet apparatus by using methanol (70%) , through 24 hours of and evaporation by rotary evaporation apparatus 40C° for drying, and isolation phenol from Menthe spicata leaves , E. coli and Sta. aureus and C.albicans were obtained from libraries university of karbala , the antimicrobial test against (S.aureas , E.coli and C.albicans) was detected by disc diffusion methods was carried out using phenol extract at concentrations of 0.5, 1, 2, and 13 mg/ml , while Znonanoparticles was performed 0.2, 0.5, 0.8, and 1 mg/ml concentration . indicate the results the inhibition zone of phenol was 17.5 against C.albicans , 13.76 mm against E .coli, 12.87 mm against S. aureus in concentration (0.5) mg/ml. while concentration 1 mg/ml in C.albicans 18.34 , S. aureus 13.98mm , E.coli 14.98mm , while concentration 2mg/ml in C.albicans 19.21mm , S. aureus 14.98mm , E.coli 16.87 mm , concentration 3 mg/ml in C.albicans 21.65mm S. aureus 16.87 mm , E.coli (17.98) mm. While Znonanoparticles' C.albicans inhibition zone was 18.98, , 19.76 mm against E .coli, 20.76 mm against S. aureus in concentration 0.2 mg/ml, while concentration 0.5 mg/ml in C.albicans 19.32 , S. aureus 21.87mm , E.coli 20.54mm , while concentration 0.8mg/ml in C.albicans 21.87mm , S. aureus 22.87 , E.coli 16.87 mm , concentration 1 mg/ml in C.albicans 22.98mm , S. aureus 24.8 mm and E.coli 23.98 mm. The study's finding is that C.albicans was inhibited by phenol compounds at various doses., while the Znonanoparticles with various doses caused the antibacterial activity to be inhibited (S.aureas , E.coli and C.albicans).
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Milne, D. P., J. C. Curran, J. S. Findlay, J. M. Crowther i S. G. Wallis. "The Effect of Estuary Type Suspended Solids on Survival of E. coli in Saline Waters". Water Science and Technology 21, nr 3 (1.03.1989): 61–65. http://dx.doi.org/10.2166/wst.1989.0079.
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The effect of estuary type suspended solids on E.coli inactivation has been examined in laboratory experiments. The work used artificial seawater, laboratory processed suspended solids and chemostat steady-state E.coli cultures to establish the effect of these physical/chemical parameters on the viability of an E.coli population. Initial E.coli concentrations were 5 × 103. 100ml−1, the temperatures employed were 10°C and 20°C, the suspended solids concentration range was 0 – l00mgl−1, salinity range 10 – 32%o, and the experimental vessels were completely shielded from light. The introduction of suspended solids at a low concentration (ie 5mgl−1) markedly increased the survival time of E.coli at the higher salinities (ie 32% o and 27%o). Suspended solids at higher concentrations (>12.5mgl−1) provided no additional increase in survival time. At low suspended solids concentrations (≤5mgl−1) decreasing salinity increased survival time; at higher suspended solids concentration (>12.5mgl−1) salinities in excess of a critical value, around 27%o, also increased survival times.
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AL.Bayatti, Khalid K., Samia K. Mahmood i Ali A. Abdul-Abbas. "Protection of Chicken against Bacterial Infection by Immunization with Bacterial Subcellular Fractions". Al Mustansiriyah Journal of Pharmaceutical Sciences 4, nr 1 (1.06.2007): 80–85. http://dx.doi.org/10.32947/ajps.v4i1.382.
Pełny tekst źródłaStreszczenie:
The current studies were undertaken to assess the ability of various subcellular fraction of E.coli to induce protection against challenge with E.coli in chicken.Immunization with as little as 12.5 μg of cytoplasmic membrane conferred 65% protection. In addition cell wall and somatic antigen conferred 55% and 25%protection respectively. These results indicate the usefulness of cytoplasmic membrane and cell wall in the induction of active immunity against pathogenic E.coli in chicken.
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Chaudhary, Mahesh Kumar, Indrani Jadhav, Indrani Jadhav i Megha Raj Banjara. "Antibiotic Profile of Extended Spectrum Beta Lactamase Escherichia coli from Clinical Samples". Journal of Karnali Academy of Health Sciences 3, nr 2 (21.09.2020): 102–10. http://dx.doi.org/10.3126/jkahs.v3i2.31324.
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Background: Extended spectrum β-lactamases have addressed the serious challenges worldwide due to the emergence of ESBL producing genes which possess a serious threat for the treatment of infections both in community and hospitals since it is found to be increasing trends of multidrug resistance. This study was focused to find out the antibiotic profile of multidrug resistant Escherichia .coli and status of ESBLs producing E.coli.
Methods: This was a cross-sectional study conducted over a period of 2 years (September 2017 to April 2019) at microbiology laboratory of Nepal Mediciti Hospital. A total of 16542 samples were processed. Various clinical samples were collected from both inpatients and outpatients aseptically and without contaminating skin commensals. Standard microbiological techniques were used for isolation and identification of pathogens. Extended spectrum beta-lactamases were phenotypically confirmed by combined disc method.
Results: Out of 1449 E.coli isolates, 323(22.29%) were found to be MDR E.coli. Isolation rate of ESBL producing E.coli (66.56%) were found to be high among MDR E.coli isolates.
Conclusion: There was increasing prevalence of ESBL producing E.coli and was essential to monitor antibiotic susceptibility pattern and formulate antibiotic policy to prevent the spread of MDR and ESBL producers.
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Salsabila, Ghina, Lia Yulia Budiarti i Husnul Khatimah. "NILAI KOEFISIEN FENOL DAN JUMLAH KOLONI BAKTERI Escherichia coli PADA PERLAKUAN EKSTRAK KOMBINASI KULIT BUAH Citrus aurantifolia Swingle DAN Citrus hystrix DC". Homeostasis 6, nr 1 (23.05.2023): 245. http://dx.doi.org/10.20527/ht.v6i1.8812.
Pełny tekst źródłaStreszczenie:
Citrus aurantifolia Swingle (C.aurantifolia) dan Citrus hystrix DC (C.hystrix) mengandung berbagai senyawa antibakteri yang berpotensi sebagai antiseptik alternatif. Efektivitas antiseptik yang baik mempunyai nilai koefisien fenol (KF) ≥1. Penelitian ini bertujuan menganalisis aktivitas antibakteri ekstrak kombinasi kulit buah C.aurantifolia dan C.hystrix (Ca+Ch) terhadap Escherichia coli in vitro, berdasarkan parameter nilai koefisien fenol dan jumlah koloni bakteri. Rancangan penelitian posttest-only with control group design ini menggunakan metode uji dilusi untuk uji perlakuan kombinasi ekstrak Ca+Ch dan kontrol dalam menghambat pertumbuhan E.coli. Hasil uji koefisien fenol, diperoleh rerata nilai KF=1,0667 pada kombinasi ekstrak kulit buah Ca+Ch dan KF=1,0337 pada alkohol 70%; nilai KF ini setara dengan fenol5%. Hasil uji One Way-Anova, perlakuan ekstrak kombinasi kulit buah (Ca+Ch) pada variasi kombinasi 25% 50%, 75%, 100% (ratio 1:1) terhadap jumlah koloni E.coli, didapatkan rerata jumlah koloni E.coli berbeda bermakna pada perlakuan Ca100%+Ch100% dengan perlakuan lainnya (p> 0,05). Berdasarkan analisi pos-hoc Duncan, jumlah koloni E.coli dari perlakuan Ca75%+Ch100%=35,0 cfu/ml tidak berbeda bermakna dengan antiseptik alcohol 70% (34,0 cfu/ml); ekstrak kombinasi Ca100%+Ch100% menghasilkan efektivitas terbaik terhadap jumlah koloni E.coli (32,0 cfu/ml). Simpulan penelitian, ekstrak kombinasi kulit buah C.aurantifolia dan C.hystrix memiliki aktivitas antibakteri terhadap E.coli dan miliki efktivitas sebagai antiseptik.
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Tong, Wing H., Inge M. van der Sluis, Cathelijne Alleman, Raphaele RL van Litsenburg, Gertjan Kaspers, Rob Pieters i Carin A. Uyl-de Groot. "Cost-Effectiveness of Treatment of Childhood Acute Lymphoblastic Leukemia with Pegasparaginase and Erwinia Asparaginase: The Impact of Expensive Chemotherapy". Blood 120, nr 21 (16.11.2012): 4227. http://dx.doi.org/10.1182/blood.v120.21.4227.4227.
Pełny tekst źródłaStreszczenie:
Abstract Abstract 4227 Asparaginase is an expensive drug, but important in childhood acute lymphoblastic leukemia (ALL). Due to increasing costs of treatment of childhood ALL more insight in costs of asparaginase preparations is desired. In order to compare pharmacoeconomic aspects of PEGasparaginase, Erwinia asparaginase and native E.coli asparaginase, we performed a cost-effectiveness analysis in Dutch Childhood Oncology Group (DCOG) ALL-10 medium risk group (MRG) intensification protocol. Between April 2005 and October 2009, MRG patients were included in this multi-center study. Treatment costs were calculated based on patient level data of 84 subjects (33 female), and were related to allergy to asparaginase. We have used 3 treatment scenarios for asparaginase of which 2 scenarios were hypothetical: PEGasparaginase as first line preparation and Erwinia asparaginase as second line used as scenario 1 (similar to the actual ALL-10 treatment schedule); native E.coli asparaginase as first line preparation and Erwinia asparaginase as second line used as hypothetical scenario 2; or native E.coli asparaginase as first line preparation, PEGasparaginase as second line and Erwinia asparaginase as third line preparation used as hypothetical scenario 3. Medical technology assessments techniques were used for this cost-effectiveness analysis. Decision tree analysis was used to compare costs of PEGasparaginase or Erwinia asparaginase to native E.coli asparaginase, while taking into account the incidence of allergy to asparaginase and the different associated costs. Sensitivity analyses (one-way and two-way) were conducted to account for uncertainty in the used prices and calculated costs. The total costs of the ALL-10 MRG intensification course of 30 weeks were $71,147 per patient. Subgroup analysis revealed that the costs were $57,893 in patients without PEGasparaginase allergy (N=64). The costs were significantly higher ($113,558) in case of PEGasparaginase allergy (N=20) necessitating a switch to Erwinia asparaginase. The total costs were also calculated based on two weeks of asparaginase exposure; in case of PEGasparaginase the costs were $ 1,930 and for Erwinia asparaginase $ 3,785 per two weeks of treatment. Decision tree analysis showed that the treatment costs were $ 70,402 when using native E.coli asparaginase as first line preparation in intensification (scenario 3) and $ 71,809 when using PEGasparaginase as first line preparation (scenario 1). Treatment costs using native E.coli asparaginase as first line followed by Erwinia asparaginase as second line (scenario 2) the costs would be significantly higher ($ 103,474). One-way sensitivity analysis showed that the subgroup with allergy to native E.coli asparaginase (scenario 2) had the largest range in treatment costs. When treatment costs were calculated with the new European price of Erwinia asparaginase (the price has been doubled in March 2011), both treatments with PEGasparaginase as first line preparation (scenario 1) or native E.coli asparaginase as first line preparation (scenario 3) would be less expensive ($ 100,199 or $ 103,089, respectively) compared to the native E.coli asparaginase scenario 2 ($ 190,284). Two-way sensitivity analysis revealed that treatment with PEGasparaginase (scenario 1) is less expensive than treatment with native E.coli asparaginase (scenarios 2) for allergy probabilities of PEGasparaginase ranging from zero to 0.8 with a fixed allergy rate of 0.65 (Veerman et al. (Lancet Oncol. 2009)). This also holds true if a fixed allergy rate for native E.coli asparaginase of 0.4 is used, which is frequently found in studies using less native E.coli asparaginase in intensification after native E.coli asparaginase in induction (Nachman et al. (JCO 1997) and Willer et al. (Blood 2011)). In conclusion, treatment with native E.coli asparaginase, followed by a switch to PEGasparaginase, and subsequently to Erwinia asparaginase in case of allergy carried similar overall costs compared to the treatment with PEGasparaginase as first line drug (followed by Erwinia asparaginase in case of allergy). However, PEGasparaginase is preferred, because it is administered less frequently, with less outpatient care visits and is less immunogenic compared to native E.coli asparaginase. The costs with PEGasparaginase are much lower compared to Erwinia asparaginase. Disclosures: No relevant conflicts of interest to declare.
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Elbaz, Huda, Mohamed Hamed, Fatma Abdelhamid i Osama Abdalla. "Impact of cefepime on hematological, immunological and oxidant/antioxidant parameters in rats experimentally infected with E. coli ATCC 25922". Mansoura Veterinary Medical Journal 21, nr 1 (31.03.2020): 36–45. http://dx.doi.org/10.35943/10.35943/mvmj.2020.21.106.
Pełny tekst źródłaStreszczenie:
Objective: To evaluate the effect of cefepime on hematological changes, immunological disorders and hepatic oxidative damage in rats experimentally infected with E.coli ATCC 25922. Design: Randomized controlled experimental study. Animals: Thirty-two adult male albino rats weighting150-200 g. Procedures: Rats used for this study were randomly assigned into 4 equal groups: the control one, E.coli infected group (1×108CFU/I/P/once), the cefepime treated group (45 mg/kg bw/I/M/day) for 5 days and the E.coli infected group that treated with cefepime 24h after bacterial inoculation as previously described. Hematological and immunological parameters, liver function biomarkers and hepatic oxidative stress and antioxidant markers were determined. Results: Our result revealed that E.coli infection induced a significant elevation in the erythrocytes count, hemoglobin concentration, PCV% and total leukocytic count (TLC) (P < 0.05). In the same respect, liver function biomarkers, serum glucose, total cholesterol, and triglyceride levels as well hepatic malondialdehyde (MDA), nitric oxide (NO), TNF-α, IL-10, and lysozyme activity were significantly increased compared to the control rats (P < 0.05). In contrast, hepatic reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were decreased significantly (P < 0.05). Cefepime treatment in E.coli + CFPM group reduced the elevated eythrogram, TLC and liver function biomarkers. Cefepime also ameliorated the oxidative damage and inflammatory response induced by E.coli infection. Conclusion and clinical relevance: Cefepime is safe when administered in a fixed-dose and possess antioxidant that contributes to improve efficacy against adverse effect induced by E.coli ATCC 25922 infection.
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Elbaz, Huda, Mohamed Hamed, Fatma Abdelhamid i Osama Abdalla. "Impact of cefepime on hematological, immunological and oxidant/antioxidant parameters in rats experimentally infected with E. coli ATCC 25922". Mansoura Veterinary Medical Journal 21, nr 1 (3.04.2020): 36–45. http://dx.doi.org/10.35943/mvmj.2020.21.006.
Pełny tekst źródłaStreszczenie:
Objective: To evaluate the effect of cefepime on hematological changes, immunological disorders and hepatic oxidative damage in rats experimentally infected with E.coli ATCC 25922. Design: Randomized controlled experimental study. Animals: Thirty-two adult male albino rats weighting150-200 g. Procedures: Rats used for this study were randomly assigned into 4 equal groups: the control one, E.coli infected group (1×108CFU/I/P/once), the cefepime treated group (45 mg/kg bw/I/M/day) for 5 days and the E.coli infected group that treated with cefepime 24h after bacterial inoculation as previously described. Hematological and immunological parameters, liver function biomarkers and hepatic oxidative stress and antioxidant markers were determined. Results: Our result revealed that E.coli infection induced a significant elevation in the erythrocytes count, hemoglobin concentration, PCV% and total leukocytic count (TLC) (P < 0.05). In the same respect, liver function biomarkers, serum glucose, total cholesterol, and triglyceride levels as well hepatic malondialdehyde (MDA), nitric oxide (NO), TNF-α, IL-10, and lysozyme activity were significantly increased compared to the control rats (P < 0.05). In contrast, hepatic reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were decreased significantly (P < 0.05). Cefepime treatment in E.coli + CFPM group reduced the elevated eythrogram, TLC and liver function biomarkers. Cefepime also ameliorated the oxidative damage and inflammatory response induced by E.coli infection. Conclusion and clinical relevance: Cefepime is safe when administered in a fixed-dose and possess antioxidant that contributes to improve efficacy against adverse effect induced by E.coli ATCC 25922 infection.
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Elbaz, Huda, Mohamed Hamed, Fatma Abdelhamid i Osama Abdalla. "Impact of cefepime on hematological, immunological and oxidant/antioxidant parameters in rats experimentally infected with E. coli ATCC 25922". Mansoura Veterinary Medical Journal 21, nr 1 (31.03.2020): 36–45. http://dx.doi.org/10.35943/mvmj.2020.21.106.
Pełny tekst źródłaStreszczenie:
Objective: To evaluate the effect of cefepime on hematological changes, immunological disorders and hepatic oxidative damage in rats experimentally infected with E.coli ATCC 25922. Design: Randomized controlled experimental study. Animals: Thirty-two adult male albino rats weighting150-200 g. Procedures: Rats used for this study were randomly assigned into 4 equal groups: the control one, E.coli infected group (1×108CFU/I/P/once), the cefepime treated group (45 mg/kg bw/I/M/day) for 5 days and the E.coli infected group that treated with cefepime 24h after bacterial inoculation as previously described. Hematological and immunological parameters, liver function biomarkers and hepatic oxidative stress and antioxidant markers were determined. Results: Our result revealed that E.coli infection induced a significant elevation in the erythrocytes count, hemoglobin concentration, PCV% and total leukocytic count (TLC) (P < 0.05). In the same respect, liver function biomarkers, serum glucose, total cholesterol, and triglyceride levels as well hepatic malondialdehyde (MDA), nitric oxide (NO), TNF-α, IL-10, and lysozyme activity were significantly increased compared to the control rats (P < 0.05). In contrast, hepatic reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were decreased significantly (P < 0.05). Cefepime treatment in E.coli + CFPM group reduced the elevated eythrogram, TLC and liver function biomarkers. Cefepime also ameliorated the oxidative damage and inflammatory response induced by E.coli infection. Conclusion and clinical relevance: Cefepime is safe when administered in a fixed-dose and possess antioxidant that contributes to improve efficacy against adverse effect induced by E.coli ATCC 25922 infection.
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Elbaz, Huda, Mohamed Hamed, Fatma Abdelhamid i Osama Abdalla. "Impact of cefepime on hematological, immunological and oxidant/antioxidant parameters in rats experimentally infected with E. coli ATCC 25922". Mansoura Veterinary Medical Journal 21, nr 1 (3.04.2020): 36–45. http://dx.doi.org/10.35943/mvmj.2020.21.109.
Pełny tekst źródłaStreszczenie:
Objective: To evaluate the effect of cefepime on hematological changes, immunological disorders and hepatic oxidative damage in rats experimentally infected with E.coli ATCC 25922. Design: Randomized controlled experimental study. Animals: Thirty-two adult male albino rats weighting150-200 g. Procedures: Rats used for this study were randomly assigned into 4 equal groups: the control one, E.coli infected group (1×108CFU/I/P/once), the cefepime treated group (45 mg/kg bw/I/M/day) for 5 days and the E.coli infected group that treated with cefepime 24h after bacterial inoculation as previously described. Hematological and immunological parameters, liver function biomarkers and hepatic oxidative stress and antioxidant markers were determined. Results: Our result revealed that E.coli infection induced a significant elevation in the erythrocytes count, hemoglobin concentration, PCV% and total leukocytic count (TLC) (P < 0.05). In the same respect, liver function biomarkers, serum glucose, total cholesterol, and triglyceride levels as well hepatic malondialdehyde (MDA), nitric oxide (NO), TNF-α, IL-10, and lysozyme activity were significantly increased compared to the control rats (P < 0.05). In contrast, hepatic reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were decreased significantly (P < 0.05). Cefepime treatment in E.coli + CFPM group reduced the elevated eythrogram, TLC and liver function biomarkers. Cefepime also ameliorated the oxidative damage and inflammatory response induced by E.coli infection. Conclusion and clinical relevance: Cefepime is safe when administered in a fixed-dose and possess antioxidant that contributes to improve efficacy against adverse effect induced by E.coli ATCC 25922 infection.
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Elbaz, Huda. "Effect of cefepime on hematological, immunological and oxidant/antioxidant parameters in rats experimentally infected with E. coli ATCC 25922". Mansoura Veterinary Medical Journal 21, nr 1 (3.04.2020): 36–45. http://dx.doi.org/10.35943/mvmj.2020.21.116.
Pełny tekst źródłaStreszczenie:
Objective: To evaluate the effect of cefepime on hematological changes, immunological disorders and hepatic oxidative damage in rats experimentally infected with E.coli ATCC 25922. Design: Randomized controlled experimental study. Animals: Thirty-two adult male albino rats weighting150-200 g. Procedures: Rats used for this study were randomly assigned into 4 equal groups: the control one, E.coli infected group (1×108CFU/I/P/once), the cefepime treated group (45 mg/kg bw/I/M/day) for 5 days and the E.coli infected group that treated with cefepime 24h after bacterial inoculation as previously described. Hematological and immunological parameters, liver function biomarkers and hepatic oxidative stress and antioxidant markers were determined. Results: Our result revealed that E.coli infection induced a significant elevation in the erythrocytes count, hemoglobin concentration, PCV% and total leukocytic count (TLC) (P < 0.05). In the same respect, liver function biomarkers, serum glucose, total cholesterol, and triglyceride levels as well hepatic malondialdehyde (MDA), nitric oxide (NO), TNF-α, IL-10, and lysozyme activity were significantly increased compared to the control rats (P < 0.05). In contrast, hepatic reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were decreased significantly (P < 0.05). Cefepime treatment in E.coli + CFPM group reduced the elevated eythrogram, TLC and liver function biomarkers. Cefepime also ameliorated the oxidative damage and inflammatory response induced by E.coli infection. Conclusion and clinical relevance: Cefepime is safe when administered in a fixed-dose and possess antioxidant that contributes to improve efficacy against adverse effect induced by E.coli ATCC 25922 infection.
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Gautam, Dr Kirtika. "Prevalence of diarrheageanic E. coli isolated from diarrheal stool from Children under 5 years of age at Kanti Children’s Hospital". Journal of Ayurveda Campus 2, nr 1 (6.11.2021): 41–45. http://dx.doi.org/10.51648/jac.28.
Pełny tekst źródłaStreszczenie:
Background: Diarrhea caused by diarrheagenic E.coli are major health problem of developing countries including Nepal.Though precise data on childhood diarrhea by diarrheagenic E.coli in Nepal is not available. It has been estimated that approximately 25% of child death are associated with diarrhea.This study was thus carried out to evaluate the incidence of diarrhea by diarrheagenic E.coli in children under 5 years of age. The study was conducted at Kanti Children’s hospital (KCH), Maharajgunj. A total of 200 samples were enrolled between March 2018 to August 2018. Methods: All the diarrheal stool samples were cultured on Mac Conkey agar plate. After 24 hours of incubation at 37 degree celcius. If it produce Lactose Fermenting (LF) pink coloured colonies further confirmatory test was done by different biochemical test. Whether it was E. coli or not.If it was E.coli then further sub cultured on Mac Conkey Sorbitol (MSA) agar to isolate diarrheagenic E. coli. Next day if there seen Non Sorbitol Formenting (NSF) colonies, it was identified as diarrheagenic E. Coli. because most other E.coli strains and other Enterobacteria ferment sorbitol but diarrheagenic E.coli do not ferment Sorbitol and is non sorbitol fermenting colourless colonies. Results: Out of total enrolled cases, 20 (10%) were diarrheagenic E. coli. Gentamycin, Chloramphenicol and third generation Cephalosporin showed 100% efficacy against diarrheagenic E.coli . Conclusion: The incidence of disease was more prevalent in children below 2 years. Diarrheagenic E. coli were found to be a significant cause of diarrhea under 5 years of age. Awareness of improving hygiene reduce the episode of diarrhea.
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Islam, Fahrul, Haeranah Ahmad i Sitti Saddania. "Kontaminasi Escherichia coli pada Minuman Kekinian di Pusat Kota Kabupaten Mamuju". Jurnal kesehatan komunitas (Journal of community health) 10, nr 2 (27.06.2024): 222–28. http://dx.doi.org/10.25311/keskom.vol10.iss2.1664.
Pełny tekst źródłaStreszczenie:
Popularitas minuman kekinian yang semakin meningkat menyebabkan bernjamurnya gerai–gerai minuman kekinian masyarakat Indonesia, termasuk di pusat kota Kabupaten Mamuju. Pengelolaan yang tidak higienis dan saniter dapat menyebabkan pencemaran bakteri di dalam minuman yang mengakibatkan terjadinya gangguan kesehatan. Tujuan penelitian ini adalah untuk mengidentifikasi keberadaan bakteriologis Escherichia coli (E.coli) pada minuman kekinian di pusat kota Kabupaten Mamuju dalam rangka upaya pencegahan penyakit yang berhubungan dengan kandungan E.coli yang tidak sesuai dengan peraturan. Metode penelitian menggunakan metode observasional dengan pendekatan deskriptif. Pemeriksaan bakteri E. coli pada minuman kekinian menggunakan metode Most Probable Number (MPN) yang terdiri dari presumtive test (uji penduga), dan confirmed test (konfirmasi). Pengambilan sampel menggunakan pendekatan purposive sampling dengan mengambil sampel pada 11 pedagang minuman kekinian dengan kedai permanen dan 11 pedagang minuman kekinian dengan kedai kontainer yang ada di pusat kota Kabupaten Mamuju. Penelitian ini dilaksakan pada bulan Juni-September 2023. Hasil penelitian: pada 11 sampel minuman dari kedai kontainer kedai kontainer sebanyak 91% (n=10) yang positif dan 9% (n=1) yang negatif terdeteksi keberadaan E.coli, sedangkan pada jenis kedai permanen 73% (n=8) yang positif dan 27% (n=3) yang negatif terdeteksi keberadaan E.coli. Kesimpulan: Sebagian Besar minuman kekinian yang beredar di Pusat Kota Kabupaten Mamuju positif mengandung Bakteri E.coli. Keberadaan E.coli dalam makanan dan minuman menandakan tingkat sanitasi yang kurang memadai dan mengindikasikan kemungkinan terjadinya kontaminasi oleh tinja manusia sehingga dapat menyebabkan berbagai penyakit pada saluran pencernaan. Saran: sebaiknya dilakukan pemeriksaan bakteri E.coli sampai ke uji penegasan. Sebaiknya dilakukan upaya pengawasan terhadap hygiene sanitasi minuman kekinian secara berkala dan terprogram oleh Dinas Kesehatan.
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Samis, John A., Kenneth A. Stewart, Fletcher B. Taylor i Michael E. Nesheim. "A Rapid and Sustained Increase in Neutrophil Elastase Levels Correlates Temporally with Decreased Coagulation Factor Activity during E.coli-Sepsis in Baboons." Blood 104, nr 11 (16.11.2004): 1032. http://dx.doi.org/10.1182/blood.v104.11.1032.1032.
Pełny tekst źródłaStreszczenie:
Abstract Infection is the most common pathology resulting in disseminated intravascular coagulation (DIC) in humans. Disturbances of the cell interactions between neutrophils and endothelial cells are an integral part of the pathophysiology of E.coli-sepsis. Although neutrophil elastase (NE) is required for the destruction of gram negative bacteria, dysregulated NE activity towards coagulation factors may contribute to the coagulopathies that accompany infections if its activity is not properly regulated by alpha-1-protease inhibitor (A1PI). The temporal relationships between these variables were studied in plasma samples from three baboons before and after infusion of either two sublethal doses (2.50x106 cfu/kg and 1.10x108 cfu/kg) or a lethal dose (6.50x108 cfu/kg) of E.coli. In comparison to pre-infusion levels, the A1PI/NE complex levels increased by up to 17-fold after 2hrs and remained 6-fold above baseline after 53hrs as the amount of E.coli was increased. In contrast, the A1PI activity and specific activity both decreased by up to 30% during the 2–6hrs following the lethal dose of E.coli. Large transient decreases, of between 60–96%, in the factor V and VIII activity and fibrinogen levels occurred only during the 2–6hrs following infusion of the lethal dose. In addition, there was a 70–80% decrease in the factor II, VII, IX, and X clotting activity after 6–24hrs as the dose of E.coli was increased. In conclusion, there is a temporal association between the changes in A1PI activity, the appearance of the A1PI/NE complex, and the decrease of coagulation factor activity during E.coli-sepsis in baboons. Further, the sustained elevation of the A1PI/NE complexes beyond 48hrs, even after sublethal challenge with E.coli, indicates that a persistent increase in the neutrophil activity occurs as part of the inflammatory response to bacterial infection in this animal model. This study demonstrates that the neutrophil/endothelial interaction continues far beyond the first margination stage into the second ischemia/reperfusion stage during E.coli-sepsis. The results raise the possibility that clotting factor degradation/inactivation by NE as well as consumption via the coagulation cascade occurs following infusion of sublethal and lethal doses of E.coli.
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Das, Biswadip, Subrata Chattopadhyay i Chanchal Das Gupta. "Reactivation of denatured fungal glucose 6-phosphate dehydrogenase and E.coli alkaline phosphatase with E.coli ribosome". Biochemical and Biophysical Research Communications 183, nr 2 (marzec 1992): 774–80. http://dx.doi.org/10.1016/0006-291x(92)90550-5.
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Rizvi, S., D. A. Harbour, G. R. Pearson, D. Patel, C. R. Stokes i B. G. Miller. "The use of hyper-immunised egg as a source of prophylactic antibodies in the neonatal piglet". Proceedings of the British Society of Animal Science 2001 (2001): 23. http://dx.doi.org/10.1017/s1752756200004051.
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Gastrointestinal infection caused by pathogenic bacteria and viruses are an important cause of diarrhoea and ill-thrift in human and animal neonates (Guerrant et al., 1986, Radostits et al., 1994). Escherichia coli (E.coli) and Rotavirus are both important causes of neonatal diarrhoea, in addition E.coli is an important factor in the post-weaning diarrhoea syndrome seen in early weaned piglets (Radostits et al., 1994). Neonates reared on maternal milk are protected by antibodies (IgA in humans and pigs, IgG in ruminants) which act passively in the gut against organisms which cause gastrointestinal disease. This study investigated the protective effect of egg antibodies (Lohmann Animal Health) against E.coli and Rotavirus challenge in neonatal piglets. The eggs were sourced from hens vaccinated against E.coli and Rotavirus.
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Ulfa, Evi Umayah. "In Silico Design Gene Encoding CYP71AV1 for Expression in Escherichia coli". Journal of Bio-Molecule Research and Engineering 1, nr 1 (29.07.2022): 1–6. http://dx.doi.org/10.20473/jbiome.v1i1.35856.
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Cytochrome P450 monooxygenase (CYP71AV1) is a crucial enzyme in the artemisinin biosynthesis pathway. This enzyme oxidized Amorpha 4,11 diene to produce artemisinic acid. This study aimed to in silico design high-level expression of CYP71AV1 in the E.coli system. In silico techniques are highly suitable for designing protein recombinant production before entering the laboratory. The amino acid sequence of CYP71AV1 was back-translated to the DNA sequence and adapt to E.coli codon usage by using Gene Designer. The DNA sequence of optimized CYP71AV1 was analyzed using Rare Codon Analysis to assess the expression efficiency in E.coli. The protein solubility prediction was determined using the SoDoPe tool. The optimized CYP71AV1 was determined to have a CAI 0.81, a GC content of 53.08 %, CFD with low frequency, and no negative cis or repeat elements. The result of the probability of solubility of CYP71AV1 was 0.6207 when expressed in E.coli. The MBP fusion partner can be used to increase the solubility of CYP71AV1. The in silico results showed the possibility of high-level protein expression of optimized CYP71AV1 in the E.coli system.
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Maysarah, Hilda, Rika Apriani i Misrahanum Misrahanum. "ANTIBACTERIAL ACTIVITY TEST OF ETHANOL EXTRACT OF WHITE AND RED FLESH FROM GUAVA LEAF ( Psidium guajava. L) AGAINTS Staphylococcus aureus AND Escherichia coli". Jurnal Natural 16, nr 1 (1.03.2016): 51–56. http://dx.doi.org/10.24815/jn.v16i1.4818.
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An antibacterial activity test of ethanol extract of white and red flesh from guava leaf (Psidium guajava. L) against S.aureus and E.coli; using agar diffusion method was carried out in order to produce the extract. The extract was collected using maceration method. The concentration of extract was 7,8125; 6,1035; 5,00; 4,8828; 4,3944; and 3,90625 mg/mL. The results showed that both of extracts had antibacterial activities. Ethanol extract of white flesh of fruit guava leaf had (Minimum Inhibitory Concentration) MIC value at 5.000 mg/mL against S.aureus and 4.8828 mg/mL against E.coli. Whereas ethanol extract of red flesh of fruit guava leaf had MIC value at 4.3944 mg/mL against S.aureus and E.coli. MIC value of ethanol extract of white flesh of fruit guava leaf is equal with MIC value of clindamicin concentration at 3.00 µg/mL against S.aureus, and 1.00 µg/mL against E.coli. The MIC value of red flesh of fruit guava leaf is equal to the MIC value of clindamicin concentration at 3.00 µg/mL against S.aureus, and 1.00 µg/mL against E.coli.
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Journal, Baghdad Science. "Inhibition activity of Saccharomyces boulardii and Lactobacillus acidophilus against pathogenic E.coli isolates from Recurrent Urinary Tract Infection in women In Vitro". Baghdad Science Journal 11, nr 1 (2.03.2014): 22–32. http://dx.doi.org/10.21123/bsj.11.1.22-32.
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The aims of study is to detect the inhibitory effect of Saccharomyces boulardii and Lactobacillus acidophilus on Escherichia coli that has been isolated from recurrent urinary tract infection in women. The sensitivity of E.coli isolates to antibiotics had been studied and the most resistant E.coli isolate to antibiotics had been studied .The cup assay was used on nutrient agar and Muller-Hinton agar to detect the inhibitory activity for each S.boulardii yeast grown on YEGP media and L.acidophilus grown on MRS media in which the result showed a high inhibition activity for each of them .Also in this study the adhesion property of E.coli had been evaluated in the presence of S.boulardii at concentration of 1×109 and L.acidophilus at concentration of 1×106. Result revelead that both of them inhibit the adhesion of E.coli at 72.73% and 64.91% , respecherely these concentrations was used to measure the bacteriocin activity produced by L.acidophilus and inhibitory compounds produced by S.boulardii.The result showed there were no growth for E.coli colonies after 48 hrs of incubation.