Gotowe bibliografie tematyczne / E.coli

Gotowa bibliografia na temat „E.coli”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 31 lipca 2024

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Artykuły w czasopismach na temat "E.coli"

Najim, Najim Hadi. "Survival Enterohaemorrhagic E.coli O157in locally produce soft cheese in Baghdad city and studying the effect of some physical factors on its viability". Iraqi Journal of Veterinary Medicine 32, nr 2 (31.12.2008): 108–19. http://dx.doi.org/10.30539/iraqijvm.v32i2.744.

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A total of 24 samples of three different kinds of locally produced softcheese manufactured from raw milk of Cows , Buffaloes and Ewes( 8 samples of each) were collected at weekly intervals from differentareas of Baghdad city in the period from the beginning of December2007 till the end of January 2008.The main objective of this study was to isolats , identify andenumerate Enterohaemorrhagic E.coli O157from locally produced softcheese , besides that , to study the effect of cheese storage temperature(4 C) and different salt concentration solutions on the viability andsurvival of E.coli O157 in stored cheese .Data demonstrate that 11 out 24 of samples 45.84% were found tohave Enterohaemorrhagic E.coli O157 and their average counts were1.6× 104 CFU/gm.The result of statistical analysis demonstrate that cheese samplesmanufactured from cows raw milk had significantly ( P> 0.05) higherE.coli O157 counts and isolation rates over both cheese samples thatwere manufactured from Ewes and buffaloes raw milk .the average count of Enterohaemorrhagic E.coli O157 in cheesemanufactured from cows milk were 3.8× 104 CFU/gm and the isolationpercentage was 75% while their average counts in cheese manufacturedfrom Ewes and buffaloes milk were 7.3 ×103 & 2.4 ×103 CFU/gmrespectively and the isolation percentages were 37.5 % & 12.5%respectively.Data revealed that all soft cheese samples that were found to haveEnterohaemorrhagic E.coli O157 and stored at refrigeration temperature(4 C) had non significant ( P<0.05) reduction in the average counts ofE.coli O157after 3 weeks of storage and this indicate that the refrigerationstorage temperature (4 C) had no significant effect on the viabilityand survival of E.col O157 .All soft cheese samples that were found tohave Enterohaemorrhagic E.coli O157 were stored in brinesolutions at different salt concentrations such as 2.5%, 5%,7% and10% for 24 hr,1 week, 2weeks & 3weeks . Data revealed that brinesolution at high concentration of salt such as 10% had nonsignificant effect on the viability and survival of E.coli O157after 3weeks of storage.

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Jayanti, Devi Dwi, R. Susanti, Ari Yuniastuti i I. Wayan Suardana. "Deteksi Escherichia coli O157 pada air minum di Kelurahan Sekaran Gunungpati Semarang". Jurnal Biologi Udayana 24, nr 2 (25.12.2020): 55. http://dx.doi.org/10.24843/jbiounud.2020.v24.i02.p01.

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Penelitian bertujuan untuk mendeteksi adanya bakteri Escherichia coli O157 pada air minum kemasan, air minum isi ulang, dan air sumur di Kelurahan Sekaran Gunungpati Semarang. Sampel yang diambil sebanyak 20 sampel yang terdiri atas 4 merk air minum kemasan, 8 sampel air minum isi ulang, dan 8 sampel air sumur. Penelitian diawali dengan tahap isolasi E.coli pada medium Eosin Methylen Blue Agar (EMBA), yang dilanjutkan ke medium Sorbitol MacConkey Agar (SMAC) untuk identifikasi E.coli O157 dilanjutkan uji lateks aglutinasi (OXOID) dan diakhiri dengan uji konfirmasi gen rfbE menggunakan teknik Polymerase Chain Reaction (PCR). Hasil penelitian menunjukkan 8 sampel yang positif E.coli pada medium SMAC menunjukkan positif E.coli O157 (colorless). Uji lateks aglutinasi juga menunjukkan 8 sampel positif E.coli O157 seperti kontrol ATCC 43894. E.coli ATCC 43894 dan 8 sampel E.coli dari berbagai air minum di Kelurahan Sekaran Gunungpati Semarang menunjukkan positif E.coli O157.

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Iijima, A., T. Meguro i I. Yamato. "Thermostability of E.coli trprepressor". Seibutsu Butsuri 41, supplement (2001): S159. http://dx.doi.org/10.2142/biophys.41.s159_4.

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Kleanthous, C. "Suicidal proteins in E.coli". Biochemical Society Transactions 29, nr 3 (1.06.2001): A48. http://dx.doi.org/10.1042/bst029a048b.

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Khafizov, Rustem G., Alexander G. Kozlov, Timothy M. Lohman i Yann R. Chemla. "E.coli SSB Under Tension". Biophysical Journal 98, nr 3 (styczeń 2010): 270a. http://dx.doi.org/10.1016/j.bpj.2009.12.1470.

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Wiggins, Paul, Joshua Martin i Jane Kondev. "Chromatin Organization in E.coli". Biophysical Journal 96, nr 3 (luty 2009): 20a. http://dx.doi.org/10.1016/j.bpj.2008.12.1006.

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Wihansah, R. R. S., M. Yusuf, M. Arifin, A. Y. Oktaviana, Rifkhan Rifkhan, J. K. Negara i A. K. Sio. "Pengaruh Pemberian Glukosa yang Berbeda terhadap Adaptasi Escherichia coli pada Cekaman Lingkungan Asam". Jurnal Sain Peternakan Indonesia 13, nr 1 (10.03.2018): 29–35. http://dx.doi.org/10.31186/jspi.id.13.1.29-35.

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Setiap makhluk hidup termasuk bakteri memiliki kemampuan untuk beradaptasi pada kondisi lingkungan yang tidak sesuai dengan zona nyamannya. Penelitian ini bertujuan untuk melihat respon bakteri E.coli terhadap kondisi asam atau pH rendah sebagai bentuk adaptasinya. Pada penelitian ini terdapat 5 perlakuan yaitu E.coli yang ditumbuhkan pada media Nutrien Broth sebagai kontrol (P0), E.coli yang ditumbuhkan pada media Nutrien Broth dengan kondisi asam (pH 4) (P1), E.coli yang ditumbuhkan pada media Nutrien Broth dengan kondisi asam (pH 4) dan diberi glukosa 10 % (P2), selanjutnya diberi glukosa 30 % (P3) dan 50 % (P4). Pada setiap perlakuan diulang sebanyak 3 kali. Jumlah populasi dihitung dengan Metode Turbidimetri menggunakan spektrofotometer melalui nilai kekeruhan (OD). Hasil penelitian ini menunjukkan bahwa media dengan pH rendah (pH 4) dapat menghambat pertumbuhan bakteri E.coli, sedangkan penambahan glukosa mampu mempertahankan pertumbuhan bakteri E.coli. Penambahan glukosa sampai 50 % terbukti mampu meningkatkan populasi sampai jam ke 29, sedangkan pada pemberian glukosa 10 dan 30 % sudah terjadi penurunan populasi bakteri E.coli pada jam ke 29.Kata kunci: E.coli, adaptasi, asam, populasi bakteri

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Сэргэлэн, H., C. Лхагвасүрэн i Д. Алтангэрэл. "“ЭМГЭГ ТӨРӨГЧ E.COLI-ИЙН ХОРУУ ЧАНАРЫН ЗАРИМ СУДАЛГАА”". Mongolian Journal of Agricultural Sciences 12, nr 1 (7.12.2014): 3–7. http://dx.doi.org/10.5564/mjas.v12i1.244.

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Манай оронд өргөн дэлгэр тархсан (ундны ус, хүнс тэжээл, мал, ахуйд. г.м) E.coli бүлгийн нянгийн эмгэг төрүүлэгч хэв шинжийг тодорхойлох, оношлогооны технологийг боловсронгуй болгох нь хүнсний халдвар хордлогоос сэргийлэх арга зам юм. Энэхүү судалгаагаар хүн, мал эмнэлэгийн лабораторийн шинжилгээ, судалгааны ажлын үр дүнгээр олдсон E.coli-ийн 24 өсгөвөрийн 79.1 % (19), E.coli-ийн Mal B генд эерэг, 7 % (5) нь eae генийн өвөрмөц дараалалд эерэг байна. Молекул эпидемиологийн судалгаагаар мах түүнд хамаарагдах гадаад орчноос ялгасан E.coli-ийн 16,7% хоолны хордлоготой өвчтнөөс ялгасан E.coli-ийн 25% нь EHEC байна.DOI: http://dx.doi.org/10.5564/mjas.v12i1.244

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Silverman, Lewis B., Donna Neuberg, Jeffrey Supko, Mary Relling, Christina Woodward i Stephen E. Sallan. "Pharmacodynamics and Tolerability of Twice-Weekly Erwinia Asparaginase after E. coli Asparaginase Allergy in Children with ALL." Blood 108, nr 11 (16.11.2006): 1857. http://dx.doi.org/10.1182/blood.v108.11.1857.1857.

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Abstract BACKGROUND: E.coli L-asparaginase (ASP) is an important component of multiagent chemotherapy for childhood ALL; however, allergic symptoms develop in 25–30% of patients (pts). Erwinia ASP is an alternative preparation which has been used after E.coli ASP allergy; however, it has not been previously shown how well E.coli ASP-allergic pts tolerate Erwinia ASP or whether they achieve therapeutic ASP levels. METHODS: On DFCI ALL Consortium Protocol 00–01 (2000–2005), newly diagnosed children with ALL (aged 1–18 years) received 30 weeks (wks) of IM ASP during consolidation beginning 7 weeks after diagnosis. All pts initially received weekly E.coli ASP. Nadir serum ASP concentrations were measured every 3 wks by a validated biochemical assay and antibodies to E.coli and Erwinia ASP were measured by ELISA every 6 wks. If E.coli ASP allergy developed, children received Erwinia ASP (25000 IU/m2/dose twice weekly) until 2003, when Erwinia ASP became unavailable. RESULTS: We analyzed the data of all 44 patients treated between 2000–2002 who received Erwinia ASP after E.coli ASP-allergy. Median age at diagnosis was 5.5 years. Median duration of E.coli ASP prior to development of allergy was 5 wks (range 1–23). 26 (59%) pts were positive for E.coli ASP antibodies. Erwinia ASP toxicities included: allergy in 15 (34%) pts (which occurred a median 8 wks after starting Erwinia, range 2–15.5), pancreatitis in 1 (2%) and insulin-requiring hyperglycemia in 1 (2%) pt. 18 (41%) pts became positive for Erwinia ASP antibodies, 4 of whom developed Erwinia ASP allergy. Highest nadir ASP concentrations observed after Erwinia ASP in the 44 E.coli-allergic pts are displayed in the Table (below). Prior data has suggested that a nadir serum ASP level of >=0.1 IU/mL is associated with asparagine depletion (therapeutic level). Nadir Serum ASP Levels after Erwinia ASP in E.coli ASP-allergic pts N Median (range) ASP Level (IU/mL) % with ASP Level >= 0.1 IU/mL All E.coli ASP allergic pts 44 0.231(0.00–3.33) 84% Subsequent allergy to Erwinia Yes 15 0.418 (0.00–3.33) 80% No 29 0.215 (0.00–0.93) 86% E.coli ASP antibody positive 26 0.171 (0.00–3.33) 73% negative 18 0.318 (0.10–0.93) 100% Erwinia ASP antibody Positive 18 0.171 (0.00–3.33) 72% negative 26 0.322 (0.00–0.93) 92% Highest ASP concentration with E.coli ASP prior to allergy Subtherapeutic(<0.1 IU/mL) 20 0.221 (0.00–1.04) 85% Therapeutic(>=0.1 IU/mL) 24 0.323 (0.00–3.33) 83% Excluding pts who switched to PEG when Erwinia became unavailable in 2003 (N=11), pts remained on Erwinia ASP for a median of 16 wks (range 2–28). 31 pts (70%) ultimately completed all planned 30 wks of ASP consolidation. CONCLUSIONS: We conclude that twice-weekly Erwinia ASP is well-tolerated and achieves detectable and potentially therapeutic serum ASP levels in the majority of E.coli ASP-allergic pts, including those with ASP antibodies (E.coli and/or Erwinia), pts who developed subsequent allergy to Erwinia ASP and pts who never achieved therapeutic nadir levels with prior E.coli ASP. Erwinia ASP should be considered as alternative therapy for pts with ALL who develop E.coli ASP allergy.

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Hussain, Saman ,., Shaista Alam, Shahina Mumtaz, Ihsan Ullah, Momena Ali i Noor Rehman. "Antibiogram and Frequency of BLA-NDM-1 Gene in E.Coli Isolated from Carbapenem Resistant Cases of UTI Patients". Pakistan Journal of Medical and Health Sciences 16, nr 1 (31.01.2022): 1470–72. http://dx.doi.org/10.53350/pjmhs221611470.

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Background: Urinary tract infections (UTI) are very common in all the population around the world. Women are the usual victims of the super bug Escherichia coli (E.coli) and usually suffer from UTI once in a lifetime. The multi-drug resistant (MDR) E.coli is very intelligent creature and turned resistant to the antibiotics through transposons, mutations and plasmids. Most of the E.coli harbors the gene bla-NDM-1 due to which they have become highly resistant to Carbapenem antibiotics. Objectives: To find the bla-NDM-1 gene in Carbapenem resistant E.coli isolated from UTI patients. To determine the frequency of Carbapenem resistant E.coli in patients attending tertiary care hospital of Peshawar. Methodology: This study was conducted in Khyber Teaching Hospital Peshawar from January 2019 to June 2019. we collected 87 urinary E.coli isolates which were resistant to Carbapenem by disc diffusion method on Mueller Hinton Agar. Polymerase chain reaction (PCR) was done to unyield the presence of bla-NDM-1 gene in these 87 cases. Results: According to this study E.coli was the most common causative agent for UTI and females suffered more than male patients. The antibiotics Tigecycline and Colistin showed 100% sensitivity against E.coli. Some of the antibiotics like Ampicillin; Meropnem etc were 100% resistant to E.coli. The gene bla-NDM-1 turned out to be positive in 26.43% of the cases. Conclusion: This study concluded that all the E.coli isolated from UTI patients having bla-NDM-1 gene exhibit resistance to Carbapenem. These isolates are very difficult to treat and limited therapeutic options are available. Keywords: Multi- drug resistant Escherichia coli, Carbapenemases, Antibiogram, Plasmids, Polymerase chain reaction, Transposons and Mutations.

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Rozprawy doktorskie na temat "E.coli"

ALENCAR, Suelene Suassuna Silvestre de. "Translocação e bactérias marcadas com 99m técnécio na icterícia obstrutiva experimental em ratos". Universidade Federal de Pernambuco, 2001. https://repositorio.ufpe.br/handle/123456789/19708.

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Submitted by Fabio Sobreira Campos da Costa (fabio.sobreira@ufpe.br) on 2017-07-17T14:33:57Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Dissertação de Mestrado - Suelene Suassuna Silvestre Alencar.pdf: 1698152 bytes, checksum: 7bc449edbb1386a1875057dc6f3f376f (MD5)
Made available in DSpace on 2017-07-17T14:33:58Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Dissertação de Mestrado - Suelene Suassuna Silvestre Alencar.pdf: 1698152 bytes, checksum: 7bc449edbb1386a1875057dc6f3f376f (MD5) Previous issue date: 2001-01-10
Estudo realizado com o objetivo de avaliar a translocação bacteriana (TB) do trato gastrointestinal para órgãos viscerais em ratos submetidos à ligadura do ducto colédoco e submetidos à administração por via oral (gavagem) de E.coli marcada com 99mTecnécio (99mTc-E.coli). Quatro grupos de ratos foram estudados: grupo I (n=10) ligadura do colédoco, grupo II (n=10) controle ou “sham operation”, grupo III (n=12) ligadura do colédoco e gavagem com 99mTcE.coli e grupo IV (n=5) controle ou “sham operation”e gavagem com 99mTc-E.coli. Usando técnica asséptica e sob anestesia, os ratos foram submetidos à laparotomia. Nos ratos dos grupos I e III realizou-se ligadura do colédoco com fio de seda nº 3 zeros e nos ratos dos grupos II e IV apenas manipulação do colédoco com pinça de Adison (sham operation). Após sete dias de observação, os animais dos grupos I e II foram mortos e ressecados fígado, baço, linfonodos mesentéricos e pulmões para exame microbiológico (meios Agar-sangue e Agar Mac Conkey) e exame histopatológico (coloração H.E. e Tricrômico de Masson) por análise morfométrica. O nível de bilirrubina nos grupos ictéricos foi elevado em relação aos do grupo controle. A incidência de bactérias translocadas foi maior no grupo I comparada ao controle p 0,05. Nos animais dos grupos III e IV, após sete dias de observação, foi administrada por via oral (gavagem) 99mTcE.coli e após 24 Hs, os ratos de ambos os grupos foram mortos e seus órgãos retirados para contagem da radioatividade em cintilador gama. Os resultados não mostraram diferença estatisticamente significativa na captação da -E.coli entre os dois grupos (p 0,05). Porém a análise das interações grupo x órgão mostrou diferença entre os grupos ictérico e controle para os órgãos: fígado e pulmão. Os dados disponíveis permitem concluir que em ratos ictéricos por ligadura do colédoco ocorreu translocação de bactérias detectáveis por exame microbiológico. Não ocorreu translocação de bactérias com 99mTc no modelo proposto.
This study was designed to evaluate the bacterial translocation (TB) from the gastrointestinal tract to visceral organs in rats submitted to laparotomy and common bile duct ligation (CBDL). Four groups of rats were studied: group I (n = 10) CBDL; group II (n=10) control or “sham operation”; group III (n= 12) CBDL and 99mTc-E.coli and group IV (n=5) control or “sham operation” e 99mTc-E.coli. All the animals were operated with aseptic technic under intraperitoneal anesthesia with pentobarbital sodium (200mg/Kg). On 7th postoperative day the animals of groups I and II were killed with a letal dosis of anesthetic and the liver, spleen, mesenteric lynfonodes and lungs were ressecated to microbiological (Agar-blood and Agar-Mac Conkey) and histological examination (H.E. and Masson Trichromic) through morphometric analysis. On 7th postoperative day the animals of III and IV groups were submitted to 99mTc-E.coli gavage and after 24 hr they were killed and their organs were ressected. After that, the bacterial radioactivity was mensured through an Automatic count of Gama Radioative – model ANSR (Abott Laboratories). The bilirrubin levels of the jaundiced rats were elevated compared with the control groups. The incidence of bacterial translocation was higher in group I compared with control group (p 0,05). The results showed no significant differences among the jaundiced and control groups to the liver and lungs. The data allow to conclude that in jaundiced rat with ligated bile duct occurred bacterial translocation through microbiological analyses. The model proposed showed no bacterial translocation by the labeled 99mTc technic.

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Aoyama, Takashi. "Essential Structure of E.coli Promoter". 京都大学 (Kyoto University), 1987. http://hdl.handle.net/2433/86456.

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Harrington, Lesley. "Genes controlling anaerobic metabolism in E.coli". Thesis, Imperial College London, 1988. http://hdl.handle.net/10044/1/40623.

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Gene fusion technology has been exploited in this project to study the regulation of genes involved in the switch from aerobic to anaerobic metabolism in E.coli. Protein or operon fusions were created when the appropriate transducing phage inserted in frame with a target gene. XplacMu was found to be the most versatile vector, being capable of translocating the lac genes, minus their transcriptional and translational signals, to any site in the chromosome. The expression of p-galactosidase from these fusions will reflect both the transcriptional and translational activity of the target gene in response to a particular stimulus, in this case the presence or absence of molecular oxygen. Insertion of XplacMu into the control sequence of an anaerobically or aerobically regulated gene was detected by its anaerobic Lac+/aerobic Lac- (or vice versa) phenotype. Secondary mutagenesis of such fusions defines those control proteins involved in the switch by screening for a Lac+/Lac+ or Lac~/Lac? phenotype following TnlO mutagenesis. A library of fusion strains was identified by map position and phenotype and control of the anaerobically regulated fusions was further investigated. As the Fnr protein is essential for anaerobic respiration the regulation of the fusions by this pleiotropic activator protein was monitored. The expression of two fusion strains was regulated by another gene, designated adhC. The pleiotropic nature of this locus may be indicative of a second activator protein involved in the regulation of fermentative pathways. The cloning and subsequent genetic manipulation of this gene was simplified by constructing an adhC :: XplacHu fusion. Dual regulation by Fnr and AdhC was investigated, as was the regulation of anaerobic Lac+ fusions unaffected by either activator. The interrelationship of the various pathways of anaerobic metabolism was considered together with putative effector metabolites of the regulatory proteins involved.

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Holmström, Emelie. "Ni (II) absoption with recombinant E.coli". Thesis, KTH, Skolan för bioteknologi (BIO), 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-149310.

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Ren, Xiaojing, i 任晓晶. "Modeling pattern formation of swimming E.coli". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B43704001.

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Lee, Hoyoung. "Evolution of macrolide antibiotics in E.coli /". May be available electronically:, 2008. http://proquest.umi.com/login?COPT=REJTPTU1MTUmSU5UPTAmVkVSPTI=&clientId=12498.

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Ren, Xiaojing. "Modeling pattern formation of swimming E.coli". Click to view the E-thesis via HKUTO, 2010. http://sunzi.lib.hku.hk/hkuto/record/B43704001.

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Neelakanta, Girish. "Genome variations in commensal and pathogenic E.coli". [S.l. : s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=974330329.

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Barbosa, Joana Cristina Pacheco. "Lichenicidin: regulation, expression and bioengineering in E.coli". Master's thesis, Universidade de Aveiro, 2012. http://hdl.handle.net/10773/9548.

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Mestrado em Biotecnologia Molecular
A lichenicidina e um lantibiotico de classe II, naturalmente produzido por B. licheniformis I89. E constituida por dois peptidos denominados Blia e Blib. Este lantibiotico foi o primeiro a ser expresso completamente in vivo num hospedeiro Gram negativo (Escherichia coli). Neste trabalho, pretendeu-se avaliar o impacto da proteina LicR na biossintese da lichenicidina usando um sistema de expressao heterologa em E. coli. A estirpe de E. coli que nao contem o gene licR parece apresentar uma maior producao de lichenicidin do que a estirpe que contem todo o conjunto de genes envolvidos na sintese da lichenicidin. Assim, LicR parece nao apresentar qualquer funcao regulatoria em E. coli ou esta nao podera ser descrita segundo os mecanismos habituais de regulacao da producao de lantibioticos. Paralelamente um sistema de expressao foi construido para produzir cada um dos peptidos da lichenicidina separadamente, tendo sido comparados os niveis de producao de cada um dos peptidos. Este sistema foi usado com sucesso para produzir o peptido BliƒÀ mas nao apresentando qualquer vantagem sobre os sistemas ao nivel da producao. Finalmente, uma biblioteca de mutagenese do peptido Bliƒ¿ foi construida em E. coli e os clones obtidos foram analisados; a maioria dos clones obtidos apresentou bioatividade reduzida ou nula contra Micrococcus luteus. Alguns destes clones foram sequenciados para determinar qual(ais) a(s) mutacao(oes) presente(s) no gene licA1.
Lichenicidin is a class II lantibiotic, naturally produced by Bacillus licheniformis I89 strain. It is composed by two peptides: Bliα and Bliβ. This was the first lantibiotic to be fully produced in vivo using a Gram negative host (Escherichia coli). Herein, the impact of LicR protein in lichenicidin biosynthesis was assessed, using an E. coli heterologous expression system. It was shown that the E. coli strain without the licR gene presented increased lichenicidin production, when compared with the strain containing the entire gene cluster. Thus, if LicR presents some regulatory function in E. coli, its role cannot be described according to the usually proposed regulation mechanisms involved in lantibiotic production. Also, an expression system was constructed to produce each lichenicidin peptide independently and this expression system was compared with other available systems in terms of production levels. The system was successfully used to obtain Bliβ peptide. However it did not show any advantage over the systems previously developed. Ultimately, a mutagenesis library of Bliα was constructed in E. coli and the clones were analyzed; the majority of the clones showed low or null bioactivity against Micrococcus luteus. Some of these clones were sequenced to determine which mutation(s) was present in the licA1 gene.

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Green, Andrew. "The impact of combined sewer overflow removal on the environmental status of a small urban watercourse (Pymme's Brook, North London)". Thesis, University of Hertfordshire, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323428.

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At the end of 1995 work was completed on a low level intersecting foul sewer for the upper Pymme' s Brook catchment (north London), known as the East Barnet foul water sewerage scheme. Commissioned by Thames Water Utilities Limited (TWUL), it was intended that this would both resolve flooding problems in the area, and address environmental concerns raised by the Environment Agency (EA). The key element of the scheme was the removal of seven combined sewer overflows (CSOs) that the EA had defined as 'unsatisfactory'. Consequently, the present study assesses the scheme's impact on the brook's environmental status, and considers the results in light of the pollutant generation, transport and dispersal properties of the catchment. The pollutant generation, transport and dispersal processes operating in the catchment were explored at a range of spatial and temporal scales, in order to assess the contributions made by a range of urban non-point sources of pollution (CSOs, misconnections and urban runofl), under differing weather conditions, and to determine the way in which they interacted to control water quality. Considerable temporal and spatial variability was identified in the quality of both the brook, and the effluents discharging to it. A first flush of contamination was noted for both solid and dissolved pollutants, during many of the studied storm events; although the studied determinants (pH, conductivity, suspended solids, biochemical oxygen demand, dissolved oxygen, ammonia-N, chloride and E. coli count) responded to storm driven processes in different ways. A holistic approach was adopted to define the environmental status of the studied watercourse; incorporating its benthic macro-invertebrate community structure (BMWP score and ASPT), bacteriology (E. coli count) and water chemistry. Temporal change was then identified in each data set by performing an ANOVA between years, and between the periods prior to, during and after the scheme's construction. The scheme's impact on catchment hydrology was also explored by assessing temporal changes in the catchment's unit hydrograph parameters, using both linear regression for, and ANOVA between the periods related to the scheme's construction. In addition, regression analysis was used to explore the relationship between climatically induced hydrological change and both BMWP score and water column E. coli count, in which both variables were related to the mean discharge recorded at the EA's Silver Street gauging station on a range of temporal scales. It was concluded that climatically driven hydrological change was the major factor in determining the environmental status ofPymme's Brook, whereas the East Barnet foul water sewerage scheme produced only a limited improvement. This was because as well as removing several pollutant sources, the scheme had a hydrological effect that negated some of the expected improvements in water qUality. In addition, the large number and variety of pollutant sources operating in the catchment meant that a scheme designed to address just one element of the problem was unlikely to have a wtifonnly positive effect. Consequently, the magnitude of the temporal changes observed varied between the eight sites sampled in a way that was determined by a combination of the sensitivity of the benthic macro-invertebrate community inhabiting a site, the contamination processes prevalent within its local catchment area and its location within the catchment as a whole. Methodological recommendations for the future are made.

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Książki na temat "E.coli"

Burgess-Brown, Nicola A., red. Heterologous Gene Expression in E.coli. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6887-9.

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Evans,, Thomas C., i Ming-Qun Xu, red. Heterologous Gene Expression in E.coli. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61737-967-3.

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undifferentiated, Christopher Yates. Copper resistance proteins from E.coli. Birmingham: University of Birmingham, 1998.

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Ownis, Ali A. Verocytotoxin expression by E.coli O157:H7. [s.l.]: typescript, 1999.

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R, Palmer Stephen, red. E.coli: Environmental health issues of VTEC O157. New York: Spon Press, 2002.

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R, Palmer Stephen, red. E.coli: Environmental health issues of VTEC O157. New York: Spon Press, 2002.

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Stocks, Stuart Michael. The flocculation of high concentration cell debris from E.coli. Birmingham: University of Birmingham, 1997.

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Cox, Graham L. Determination: Into the E.Coli 0 157 fatal accident inquiry. [Airdrie}: s.n., 1999.

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McNeish, Iain Alexander. Virus directed enzyme prodrug therapy using E.COLI nitroreductase and CB1954. Birmingham: University of Birmingham, 1998.

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Schofield, Richard. E.coli 0157: Private wealth V public wealth? : a public interest report. Bolton: Bolton Business School, 1998.

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Części książek na temat "E.coli"

Fábry, M., P. Kasšpar, S. Zadrazžil, F. Kaprálek i J. Sedláček. "Expression of Prochymosin cDNA in E.Coli". W Metabolism and Enzymology of Nucleic Acids, 123–26. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4613-0749-5_17.

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Glass, R. E., i V. Nene. "Genetic Dissection of E.coli RNA Polymerase". W Gene Manipulation and Expression, 155–72. Dordrecht: Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-011-6565-5_12.

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Hoschützky, Heinz, Thomas Bühler, Ralph Ahrens i Klaus Jann. "Function and Molecular Architecture of E.Coli Adhesins". W Molecular Pathogenesis of Gastrointestinal Infections, 71–78. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4684-5982-1_10.

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Zeng, Xiangmiao, Ke Liu, Fangping Xie, Ying Zhang, Lei Qiao, Cuihong Dai, Aiju Hou i Dechang Xu. "A Simulation of Synthetic agr System in E.coli". W Bioinformatics Research and Applications, 76–86. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-38036-5_11.

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Hostomský, Z., i V. Pačes. "Expression of the Synthetic Proenkephalin Gene in E.coli". W Gene Manipulation and Expression, 22–32. Dordrecht: Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-011-6565-5_2.

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Fuchs, Eckart. "The Translation Initiation Signal in E.Coli and its Control". W Genetic Engineering, 15–35. Boston, MA: Springer US, 1999. http://dx.doi.org/10.1007/978-1-4615-4707-5_2.

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Weglöhner, Wolfgang, Jürgen Schmidt, Klaus Giese i Alap R. Subramanian. "Expression and Assembly of Chloroplast Ribosomal Proteins in E.Coli". W The Translational Apparatus, 701–11. Boston, MA: Springer US, 1993. http://dx.doi.org/10.1007/978-1-4615-2407-6_66.

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Grunberg-Manago, Marianne, i Alexander von Gabain. "Enzymes Involved in Control of mRNA Decay in E.Coli". W Post-transcriptional Control of Gene Expression, 9–35. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-60929-9_2.

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Aguirre, G., T. López, P. Quintana, D. Aguilar i A. Ortega. "E.coli Geneticaly Modified and Encapsulated in Sol-Gel Silica". W Emerging Fields in Sol-Gel Science and Technology, 437–45. Boston, MA: Springer US, 2003. http://dx.doi.org/10.1007/978-1-4615-0449-8_45.

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Oliva, A. M., A. Homs, E. Torrents, A. Juarez i J. Samitier. "Effect of Electric Field and Temperature in E.Coli Viability". W IFMBE Proceedings, 1833–36. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-00846-2_452.

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Streszczenia konferencji na temat "E.coli"

Shvalov, Alexander N., Valeri P. Maltsev, Galina V. Kochneva i Galina F. Sivolobova. "Light-scattering properties of E.coli and E.coli infected by phage". W Biomedical Topical Meeting. Washington, D.C.: OSA, 1999. http://dx.doi.org/10.1364/bio.1999.bwb7.

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Ananya, Namburi, Puram Ravi Kumar, D. N. Asritha i Ch Usha Kumari. "DNA Classification For Finding E.coli". W 2024 3rd International Conference on Applied Artificial Intelligence and Computing (ICAAIC). IEEE, 2024. http://dx.doi.org/10.1109/icaaic60222.2024.10575339.

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Gunda, Naga Siva Kumar, Selvaraj Naicker, Maryam S. Ghoraishi, Subir Bhattacharjee, Thomas G. Thundat i Sushanta K. Mitra. "Microspot With Integrated Wells (MSIW) for the Detection of E.coli". W ASME 2013 11th International Conference on Nanochannels, Microchannels, and Minichannels. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/icnmm2013-73037.

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There is an increasing problem in getting quality water for developing countries. Water system is contaminated and without proper treatment, it has been consumed as drinking water. It is a big problem for health. Escherichia coli (E.coli) is the main cause for the contamination of water and illness in people. Early detection of E.coli presence in the drinking water followed by subsequent treatment for elimination of E.coli can solve this problem. The present work developed a new method for detecting E.coli in contaminated water using microspot with integrated wells (MSIW). The method involves the fabrication of MSIW, coating the MSIW with enzyme substrates such as 4-MUG substrate (4-Methylumbelliferyl-β-D-glucuronide, trihydrate) and Red-Gal substrate (6-Chloro-3-indolyl-β-D-galactoside) in proper medium and dispensing the contaminated water into MSIW. GlucuronidaseA (gusA) gene in E.coli encodes the beta-D-Glucuronidase (GUS) to hydrolyze the substrate 4-MUG enzymatically which leads to the generation of the fluorigenic compound 4-MU. β-galactosidase enzyme in E.coli produces red color when it reacts with Red-Gal substrate. Using portable optical readers, average color/fluorescence intensity emitting by MSIW is measured and quantified. Comparing obtained intensity values with calibrated intensity values, the level of contamination can be predicted for early warnings.

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Гущина, Е. А., О. В. Бакланова, О. А. Лопатина, Ф. В. Лисицын, Л. С. Федорова, М. В. Бидевкина, М. В. Мезенцева i М. А. Силичева. "ВЛИЯНИЕ ГЕТЕРОПОЛИКИСЛОТ КЕГГИНА НА УЛЬТРАСТРУКТУРУ E.COLI". W НАУЧНЫЕ ОСНОВЫ ПРОИЗВОДСТВА И ОБЕСПЕЧЕНИЯ КАЧЕСТВА БИОЛОГИЧЕСКИХ ПРЕПАРАТОВ ДЛЯ АПК. Москва: ООО «Август Борг», 2020. http://dx.doi.org/10.47804/978-5-89904-028-3_2020_361.

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Theegala, Chandra S., Beniam T. Berhane i Ahmad A. Suleiman. "A Piezoelectric Crystal Immunosensor for E.coli". W International Conference On Environmental Systems. 400 Commonwealth Drive, Warrendale, PA, United States: SAE International, 1997. http://dx.doi.org/10.4271/972423.

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Wei Liu i Aiping Wu. "Uncover protein complexes in E.coli network". W 2015 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2015. http://dx.doi.org/10.1109/bibm.2015.7359844.

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Han Zhong Ke, Jing Ren, Xiao Lan Li, Huai Han Cai, Hao Yu, Ching Song i Max Song. "Artificial bile acid receptor in E.coli". W 2011 International Conference on Remote Sensing, Environment and Transportation Engineering (RSETE). IEEE, 2011. http://dx.doi.org/10.1109/rsete.2011.5964028.

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Sharifullina, D. T., R. N. Nizamov, R. N. Nizamov, I. R. Yunusov i G. I. Rakhmatullina. "STUDYING THE POSSIBILITY OF JOINT CULTIVATION OF B.BIFIDUM AND E.COLI ON ADAPTED NUTRIENT MEDIA". W STATE AND DEVELOPMENT PROSPECTS OF AGRIBUSINESS Volume 2. DSTU-Print, 2020. http://dx.doi.org/10.23947/interagro.2020.2.423-426.

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Microbial substances introduced into the body of animals increase radio stability and reduce the mortality rate. The greatest significance can be obtained by using vaccines based on bacteria of the intestinal-typhoid group, which in the process of life produce antibacterial substances, enzymes, antigens, entero-and exotoxins, and cytokines with radioprotective properties. The tests revealed a complex mechanism of interaction between bifidobacteria and Escherichia in their joint cultivation. The biomass accumulation of E.coli strain «PL-6» and B.bifidum 1 during co-cultivation depended on the ratio of live bacteria E.coli strain «PL-6» and B.bifidum 1. Microcopy of smears made on days 1-4 from monocultures showed that the grown microbes in morphology corresponded to these cultures. The concentration of microorganisms, determined by tenfold dilution by the above method, was 1x109 CFU/ml - E.coli and 1x107 CFU/ml B.bifidum, with a sowing dose of each type of microbe 1x108 CFU/ml. Microcopy of smears made from a mixture of cultures showed that a dilution of 0,9:1,1-1,0:1,0 is most optimal for co-growing bifidum and Escherichia coli, since with a relatively equal number of monocultures on the 1st day Escherichiae multiply intensely, splitting the components of the Blaurock medium and inhibiting the growth of bifidum, but from the 3rd day B.bifidum begins to prevail, splitting E.coli and assimilating substances cleaved by E.coli.

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Iseri, Emre, Seref Akay i Wouter van der Wijngaart. "Detection of E.coli in a digital assay". W 2018 IEEE Micro Electro Mechanical Systems (MEMS). IEEE, 2018. http://dx.doi.org/10.1109/memsys.2018.8346545.

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Svartholm, E., U. Haglund, J. Ljungberg i U. Hedner. "THE EFFECT OF APROTININ ON EXPERIMENTAL PORCINE SEPTIC SHOCK". W XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644243.

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Septic shock induces pronounced haemodynamic changes resulting in pulmonary microemboliz., complement activ. and an activ. of the coag. and fibrinol. systems. Only minor effects on the sepsis-induced changes were found on pretreatment with a variety of platelet aggr. inhib. (Svartholm et al 1986). Aprotinin inhibits among other enzymes kallikrein. Endotoxin shock was induced in 12 pigs by i .v. inf. of E.coli, six of vdiich got Aprotinin i.v. (1000000 KIE) immediately before the E.coli infusion. Another 6 pigs were used as controls. Haemodynamic parameters, platelet counts, fibrinol. act., fibrinogen, ATIII, and ethanol gel. test were measured before the infusions, 8,60, 90-120, 150-180 min after. Ihe haemodynamic parameters stayed unchanged in the control pigs. In the E. coli-treated animals cardiac output and the mean art. pressure decreased signif. On the other hand, the mean pulm. art. blood pressure and the pulm. vase, resist, increased signif. Ihe increase of the pulm. resist, was less pronounced and reversible in the Aprotinin treated animals. Ihe pulm. vase, resist, in the E.coli group increased contin. from 2.8ଐ.2 mm Hg before to a max. of 23.4 mm Hg at the end of the obs. time and in the Apro-tinin-treated animals from 2.0ଐ.3 to 6.6଑.9 mm Hg (8 min after the inj.) being down to 4.4ଐ.2 at the end of theexp.The platelet decrease was less pronounced in the Aprotinin pretreated group, the ethanol gel test turned pos. in the E.coli group after 60 min but stayed neg. in both the contr. and the Aprotinin pretreated animals. Fibrinolytic act, was seen in all E. coli-treated animals but stayed neg. in the contr. Moderate changes were seen in ATIII and fib. gen in all E.coli-treated pigs. All animals treated with only E.coli died at the end of the obs. time but none in the control group and none in the Aprotinin pretreated groupIn conclusion Aprotinin seems to prevent the secondary fatal increase in pulm. resist, most probably caused by pulm. oedema and a gen. activ. of the coag. syst. (ethanol gel. test stayed neg.)

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