Rozprawy doktorskie na temat „Direct sequencing”
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Fillion, Bergeron Marianne. "Plasmid isolation and purification by electrofiltration and comparison of different direct colony sequencing methods and PCR-based sequencing methods". Thesis, University of Ottawa (Canada), 2002. http://hdl.handle.net/10393/6050.
Pełny tekst źródła梁秀敏 i Sau-man Leung. "Direct detection of rifampin-resistant mycobacterium tuberculosis in clinical specimens by DNA sequencing". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31970102.
Pełny tekst źródłaLeung, Sau-man. "Direct detection of rifampin-resistant mycobacterium tuberculosis in clinical specimens by DNA sequencing". Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B2329498X.
Pełny tekst źródłaOtten, Nathalie. "DNA of bovine papillomavirus type 1 and 2 in equine sarcoids : PCR detection and direct sequencing /". [S.l.] : [s.n.], 1992. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.
Pełny tekst źródłaRocha, Ana Laís Bignotto da. "Sequenciamento direto dos genes SIX3, SHH, TGIF1, ZIC2 e array-CGH no estudo de pacientes com holoprosencefalia". Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/61/61132/tde-12112013-150520/.
Pełny tekst źródłaObjective: Analyze through direct sequencing technique the presence of molecular changes on the genes SHH, SIX3, ZIC2 and TGIF1 on individuals with clinical diagnosis of HPE. Analyze through array-CGH technique the presence of molecular changes on individuals with clinical diagnosis of HPE previously submitted to the direct sequencing analyzes. Local: Genetics and Human Cytogenetics Laboratory, HRAC/USP, Bauru-SP. Methods: Were selected 50 individuals from both genders with ages between 03 months and 50 years clinically diagnosed with HPE. Everyone was analyzed through the direct sequencing technique for the genes SHH and TGIF1 completely and for the genes ZIC2 and SIX3 partially. From those individuals which did not have shown changes on the direct sequencing technique, eight individuals with more severe phenotype were selected to the analysis through array-CGH. Results an Discussion: Were analyzed 50 individuals through the technique of direct sequencing of the genes SHH and TGIF1, were found two pathogenic variants in the analysis of SHH gene, in the case 1, the variant p.G24P was identified, and in the case 2 was identified the variant c.1031delC. On the TGIF1 gene were found five polymorphisms already described on the literature. Was identified a new silent variant on the exon 1 of the ZIC2 gene p. Q46Q(c.431G>A) and a polymorphism already described in the literature in two individuals on the gene SIX3. The analysis through array-CGH revealed the presence of one microdeletion in the case 37, of 1,5 Mb on the region 17p12 between the genomic positions 14,052,279-15,102,307. The same deletion was detected in the mother, though this region was never associated to the HPE. Conclusion: The direct sequencing technique is a very important tool for the molecular diagnosis of the HPE, and the direct sequencing standardization for the genes ZIC2 and SIX3 might help in more precise diagnostics on HRAC/USP future studies. The employ of new techniques such as array-CGH may indicate new relations between chromosomal regions and the multiple hit involved in the development of HPE.
Vander, Wiede Jamie. "Direct, hands-on or inquiry instruction a study of instructional sequencing and motivation in the science classroom". Master's thesis, University of Central Florida, 2011. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/5073.
Pełny tekst źródłaID: 030423060; System requirements: World Wide Web browser and PDF reader.; Mode of access: World Wide Web.; Thesis (M.A.)--University of Central Florida, 2011.; Includes bibliographical references (p. 42-48).
M.A.
Masters
Teaching, Learning and Leadership
Education
Applied Learning and Instrucation
Koziol, Adam. "Application of Direct-sequencing Peptide Proteomics to the Characterization of Antagonistic (Endogenous and Exogenous) Proteins in Cereal Grains". Thèse, Université d'Ottawa / University of Ottawa, 2013. http://hdl.handle.net/10393/23853.
Pełny tekst źródłaNguyen, Thuy. "Ultra-deep sequencing applications in virology research". Electronic Thesis or Diss., Sorbonne université, 2018. http://www.theses.fr/2018SORUS282.
Pełny tekst źródłaThe two RNA viruses HIV and HCV are getting a lot of public health concerns because both of them have overlapping risk factors for transmission through direct blood and sexual contacts. Furthermore, HIV and HCV infections are the leading cause of mortality and morbidity globally due to related diseases. However, with the introduction of antiretroviral therapy (ART) for the treatment of HIV infection and direct-acting antivirals (DAAs) for the treatment of HCV infection, patients infected by these viruses are witnessing significant improvement in their quality of life. However, the high replication rate and the lack of error correction mechanism of these viruses result in a diverse viral population referred to as quasispecies. Under drug- selective pressure, the viral quasispecies select resistance variants against corresponding drug and render the therapy ineffective especially in cases an appropriate treatment monitoring is not ensured.To reserve a wide range of possibilities for a life-long ART in HIV-infected patients and in parallel to reduce cost for treatment of both HIV and HCV infection, research focusing on detection, surveillance and transmission of resistance mutations is fundamental to prevent treatment failure on antivirals. In this PhD, we employed the ultra-deep sequencing (UDS) or next-generation sequencing (NGS) technologies to look for minority resistant variants (MiRVs) which are conventionally considered to represent less than 15%-25% of viral population and undetectable by Sanger sequencing. The presence of MiRVs at baseline is possibly responsible for the treatment failure and their presence at failure may limit options for subsequent therapies. In this PhD, we evaluated the prevalence and clinical impact of MiRVs on integrase gene in HIV-infected patients failing an integrase inhibitor containing regimen. We also evaluated the impact of MiRVs in HCV genotype 3 and genotype 4-infected patients failing DAAs. Furthermore, we used the UDS technique to identify and characterize the HCV transmission networks among a key population of men having sex with men either co-infected with HIV or at high risk of HIV acquisition. We also discovered several cases of mixed HCV genotype infections in this population probably for their high risk of multiple HCV exposures. The advantages of UDS in virology research and the applicability of this technique in clinic have been questioned and verified throughout multiple types of projects in this PhD. UDS has not been conclusively established to be more interesting and beneficial than Sanger sequencing in prevention of treatment failure in patients infected by HIV or HCV and in identifying the viral transmission networks at large scale if taking into account the experiment cost and time for data analysis. However, the dynamic development of UDS technologies and the continuing attempts in optimizing analysis procedures display a promising role of UDS. And the applicability of UDS in clinical practice still needs to be elucidated in different kinds of research projects
Engström, Erik. "Direct poly(A) RNA nanopore sequencing on the freshwater duck mussel Anodonta anatina following exposure to copper : A pilot study". Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-17508.
Pełny tekst źródłaBusson, Laurent. "Evolution of direct diagnostic techniques in Virology; analytical performances and clinical input". Doctoral thesis, Universite Libre de Bruxelles, 2020. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/313391.
Pełny tekst źródłaDoctorat en Sciences médicales (Médecine)
info:eu-repo/semantics/nonPublished
Takeda, Haruhiko. "Evolution of multi-drug resistant HCV clones from pre-existing resistant-associated variants during direct-acting antiviral therapy determined by third-generation sequencing". Kyoto University, 2018. http://hdl.handle.net/2433/232107.
Pełny tekst źródłaMayrthaler, Laura [Verfasser], i Josef [Akademischer Betreuer] Eberle. "Next Generation Sequencing as screening method for resistance associated variants in Hepatitis C Virus before treatment with direct acting antivirals / Laura Mayrthaler ; Betreuer: Josef Eberle". München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2019. http://d-nb.info/1196528918/34.
Pełny tekst źródłaRUSSO, C. LO. "ANALISI COMPARATIVA DI METODICHE MOLECOLARI PER LA DETERMINAZIONE DELLO STATO MUTAZIONALE DI EGFR IN PAZIENTI CON CARCINOMA DEL POLMONE NON A PICCOLE CELLULE". Doctoral thesis, Università degli Studi di Milano, 2014. http://hdl.handle.net/2434/231152.
Pełny tekst źródłaWalbert, Katrin. "Ectomycorrhizal communities associated with a Pinus radiata plantation in the North Island, New Zealand". Lincoln University, 2008. http://hdl.handle.net/10182/658.
Pełny tekst źródłaCorbin, Alastair Lawrence. "IRF5 directs colonic inflammation and control of mononuclear phagocyte adaptation to the tissue environment". Thesis, University of Oxford, 2017. http://ora.ox.ac.uk/objects/uuid:fb846ef1-e2a4-476f-a8f8-b52ef776ef41.
Pełny tekst źródłaSilva, Tiago Roque Benetoli da [UNESP]. "Adubação nitrogenada e resíduos vegetais no desenvolvimento do feijoeiro (Phaseolus vulgaris L.) em sistema de plantio direto". Universidade Estadual Paulista (UNESP), 2002. http://hdl.handle.net/11449/98869.
Pełny tekst źródłaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
A implantação da cultura do feijão no sistema de plantio direto vem crescendo em todo território nacional, entretanto, o plantio direto exige uma adubação nitrogenada diferenciada, tornando-se necessário buscar mais informações a esse respeito. Nesse contexto, o trabalho foi desenvolvido em um LATOSSOLO VERMELHO Distrófico (LVd), em Selvíria - MS, com o objetivo de verificar o efeito da aplicação de diferentes níveis de nitrogênio em cobertura (0, 30, 60, 90, 120 e 150 kg ha-1), na cultura do feijão de inverno em sistema de plantio direto, após diferentes coberturas vegetais (milho, milheto, arroz, mucuna-preta, soja, Crotalaria juncea e milho + mucuna-preta). O delineamento experimental foi o de blocos casualizados em um esquema de parcelas subdivididas, com 4 repetições. Foram avaliadas: cobertura vegetal da cultura anterior, matéria seca das plantas, população de plantas, componentes produtivos (número de vagens e de grãos por planta, número de grãos por vagem e massa de 100 grãos), N total na parte aérea e nos grãos e, produtividade de grãos. Concluiu-se que A Crotalaria juncea e mucuna-preta proporcionaram a melhor cobertura do solo no período avaliado, com a Crotalaria juncea apresentando a maior quantidade de matéria seca (15.201 kg ha-1), o que é um fator importante no sistema de plantio direto; as diferentes coberturas vegetais não influenciaram significativamente a produtividade do feijoeiro cultivado em sucessão; a aplicação de doses crescentes de nitrogênio em cobertura aumentou a produtividade do feijoeiro, entretanto mesmo onde não houve aplicação do fertilizante a produtividade foi superior a 2000 kg ha-1, de forma que uma análise econômica passa a ser um fator importante para a tomada de decisão.
The no tillage system in common bean is improving in Brazil, however, there is controversy about sidedressing nitrogen fertilization, so is need more information about it. In this context, the experiment was carried out on a Typic Haplustox, in Selvíria, MS, Brazil, with the objective of evaluating the sidedressing nitrogen effects (0, 30, 60, 90, 120 and 150 kg ha-1), in common bean on no tillage system under different crop residues (corn, millet, rice, black velvet bean, soybean, crotalaria and corn + black velvet bean). The used design consisted of randomized blocks in a split-plot scheme and four replications. Crop residues, number of days to the bloom, dry matter weight, stand, number of pod and seeds per plant, number of seeds per pod, weight of 100 seeds, N in leaves and seeds and yield were evaluated. The results showed that: the Crotalaria juncea and black velvet bean provided the most crop residue, and the Crotalaria juncea with a highest dry matter weight (15.201 kg ha-1), it is a important factor on the no tillage system; there was not crop residues influence under common bean yield; the sidedressing nitrogen application increased the production, however where there was not N application the yield were higher to 2000 kg ha-1, with the economic analysis is the important to decision.
Lima, Filho Diogo Ferreira. "A codificação como proteção contra a interferencia internacional em sistemas de comunicações usando a tecnica de espalhamento espectral do tipo sequencia direta". [s.n.], 1991. http://repositorio.unicamp.br/jspui/handle/REPOSIP/259784.
Pełny tekst źródłaDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Eletrica
Made available in DSpace on 2018-07-14T04:15:36Z (GMT). No. of bitstreams: 1 LimaFilho_DiogoFerreira_M.pdf: 10803002 bytes, checksum: 974014e1fff2744db1b68cdf6b65db94 (MD5) Previous issue date: 1991
Resumo: A combinação das técnicas de espalhamento espectral, entrelaçamento e correção de erros provê aos sistemas de comunicações efetivos ganhos de processamento quando estes mesmos sistemas estão sujeitos à interferência intencional. Fundamentado nesta premissa, este trabalho apresenta a partir do modelo de um sistema de comunicações sob interferência intencional um pacote computacional para a realização de simulações e conseqüentemente possibilitando efetuar análises de desempenho e comparações das diferentes estratégias de combate à interferência intencional. Em particular serão analisados os efeitos da interferência intencional do tipo pulsada no sistema de comunicações usando a técnica de seqüência direta com relação ao espalhamento espectral, um entrelaçador do tipo bloco e um código cíclico
Mestrado
Mestre em Engenharia Elétrica
Moura, Jaqueline Zanon de [UNESP]. "Distribuição espacial e plano de amostragem sequencial de Pseudoplusia includens (Walker) (Lepidoptera: Noctuidae), em dois sistemas de plantio de soja". Universidade Estadual Paulista (UNESP), 2012. http://hdl.handle.net/11449/105130.
Pełny tekst źródłaCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
O controle de pragas da soja representa até 36,50% do custo de produção. As lagartas desfolhadoras podem comprometer a produção de soja causando desfolhas de até 100%. A dificuldade de controle, associada ao aumento populacional e seriedade dos danos causados colocam a subfamília Plusiinae em destaque nacional, sendo mais abundante a espécie Pseudoplusia includens (Walker). Portanto, objetivou-se com este trabalho estudar a distribuição espacial da P. includens em dois sistemas de cultivo de soja, bem como, elaborar um plano de amostragem sequencial para a praga. Para monitoramento dos insetos foram realizadas coletas semanais através do método do pano de batida. Estudou-se a distribuição espacial do inseto, através de índices de dispersão e testes de ajuste às principais distribuições de probabilidade. Testaram-se os ajustes às distribuições de Poisson e binomial negativa. Os valores dos índices de dispersão, para lagartas de P. includens, em sistema de plantio convencional de soja, indicaram distribuição agregada para as duas categorias de lagartas (pequenas e grandes). Os resultados na área com sistema de plantio direto foram semelhantes. Desta forma, é possível afirmar que lagartas de P. includens apresentaram disposição agregada no campo independentemente do sistema de plantio. Lagartas de P. includens apresentam disposição agregada no campo independentemente do sistema de plantio. O ajuste do número de lagartas coletadas à distribuição de probabilidades binomial negativa possibilitou a elaboração de plano de amostragem sequencial para monitoramento da praga independentemente do sistema de plantio. O número mínimo de unidades amostrais necessário para tomada de decisão é quatro
The soybean pest control represents to 36.50% of the cost of production. The defoliating caterpillars may compromise the production of soy causing defoliation of up to 100%. The difficulty of control, with increased population and seriously damage the subfamily Plusiinae put into national prominence, being the most abundant species Pseudoplusia includens (Walker). Therefore, the aim of this work was to study the spatial distribution of P. includens in two systems of land use, as well as develop a sequential sampling plan for the pest. The insects were collected weekly by the ground cloth method. The spatial distribution of the insect was studied, using dispersion indices and testing the main probability distributions. Tested the adjustments to the distributions of Poisson and negative binomial. The values of aggregation index to P. includens larvae in conventionally tilled soybean indicated aggregated distribution for the two categories of larvae (small and large). The results in the area with no-tillage system were similar. Caterpillars of P. includens present aggregate provision in the field regardless of tillage system. Adjusting the number of caterpillars collected the negative binomial probability distribution enabled the development of sequential sampling plan for monitoring the pest regardless of tillage system. The minimum number of sample units required for decision making is four
Roxo, Sónia Catarina Martins. "Integração de informação secundária na modelação geoestatística da qualidade de solos em locais potencialmente contaminados. Aplicação à área subjacente de uma antiga refinaria". Master's thesis, Faculdade de Ciências e Tecnologia, 2011. http://hdl.handle.net/10362/10226.
Pełny tekst źródłaEste trabalho apresenta uma metodologia para caracterizar espacialmente a contaminação em hidrocarbonetos totais do petróleo (Total Petroleum Hydrocarbons - TPH) no local de um antigo parque de combustíveis. A metodologia proposta foi desenvolvida com algoritmos de estimação e simulação geoestatística 3D e integra informação principal e secundária. Foram utilizados dados de 131 sondagens de uma anterior campanha de prospecção, para uma área de aproximadamente 14,5ha. As variáveis secundárias são apresentadas por classes(variável categórica) relativamente a três análises expeditas macroscópicas e sensoriais ao solo recolhido das sondagens. Ao todo foram analisados e codificados 820 troços, que constituem o comprimento total das sondagens. Dos 820 troços, 276 foram enviados para análise laboratorial tendo sido reportados os teores em TPH (informação principal). Comparativamente aos dados laboratoriais, os dados das análises sensoriais são muito expeditos e, por isso, mais abundantes; todavia têm um elevado grau de subjectividade na discriminação da contaminação. Pelo contrário, as determinações analíticas são menos expeditas e muito mais caras, mas os resultados são mais fiáveis do ponto de vista de identificar a contaminação. A metodologia proposta desenvolve-se em três etapas. Em primeiro lugar, procedeu-se à análise estatística preliminar para determinar qual das análises sensoriais e respectivas classes(cor, cheiro e reacção ao óleo) seriam mais adequadas para discriminar os teores em TPH. De seguida, fez-se a estimação 3D das classes da variável seleccionada por krigagem da indicatriz. Finalmente, na terceira etapa, faz-se a simulação dos teores em TPH. Na simulação utilizou-se uma variante inovadora do algoritmo de simulação sequencial directa com condicionamento a histogramas e médias locais. A metodologia proposta foi validada com um teste de validação cruzada, construído a partir de um subconjunto aleatório de 20% das amostras.
Quirino, Geise de Aguiar. "Análise de mutações em formas recessivas de pacientes com Asteogênese Inperfeita do Espírito Santo: comparação de metodologias". Universidade Federal do Espírito Santo, 2012. http://repositorio.ufes.br/handle/10/5758.
Pełny tekst źródłaOsteogenesis Imperfecta (OI) is a genetic desease characterized by patient s bone fragility and deformity, in which severity ranges from a barely detectable connective tissue disorder to lethality in the perinatal period. The diversity of clinical variability in patients is caused by the different location or type of mutations in one of the ten genes related with the disease. This wide clinical variability difficults the perfect clinical diagnoses, due to that the use of molecular biology techniques becomes necessary to obtain a correct diagnoses and for genotype: phenotype correlation. One of the relevant genes associated with recessive forms of OI is the LEPRE-1 gene, responsible for encoding the prolyl 3 hidroxylase 1 protein. This protein and two others are components of the complex responsible for pro-collagen alfa 1 chains 3 prolyl hydroxylation. The target of this research was to analyze the LEPRE-1 gene in eight non consanguineous patients clinically diagnosed as severe Osteogenesis Imperfecta suggestive of autossomic recessive heritage by DNA sequencing of exons 1, 3, 5, 6 and 14 of the gene. In addition, the data obtained was used to analyze the efficiency of the SSCP technique by comparing the results between screening for mutations methodologies and gene sequencing methodologies. On exon 6, for instance, a mutation in one patient was found: a heterozygose base change (c.1087A>G / p.Lys363Glu), consequently, lysine was produced instead of glutamic acid. On the other exons, there was no mutation found on the patients chosen. All the results obtained in this research were compatible with datas generated by SSCP and suggest high efficient of SSCP technique for LEPRE-1 gene to recessive cases of Osteogenesis Imperfecta
A Osteogênese Imperfeita é uma doença genética caracterizada por fragilidade e deformidade esquelética, onde o quadro clínico pode variar desde simples deformidades ósseas à forma letal perinatal. A alta variabilidade clínica apresentada pelos indivíduos afetados ocorre devido ao tipo e à localização da mutação em um dos dez genes relacionados a doença. A grande heterogeneidade genética existente exige a utilização de técnicas da biologia molecular para o diagnóstico e compreensão das correlações genótipo: fenótipo da doença. Um dos genes relevantes associados com as formas recessivas da Osteogênese Imperfeita é o gene LEPRE-1 codificador da proteína prolil 3 hidroxilase 1. Esta é uma das três proteínas componentes do complexo responsável pela prolil 3 - hidroxilação das cadeias de pró-colágeno alfa 1 formadoras da molécula do colágeno tipo I, expresso, predominantemente em ossos, tendões e pele. Este projeto de pesquisa teve como objetivo analizar o gene LEPRE-1 em oito pacientes não consanguineos com Osteogênese Imperfeita tipo grave sugestivos de herança autossômica recessiva por meio do sequenciamento direto dos exons 1, 3, 5, 6 e 14 do gene. Além disso, o resultado gerado foi utilizado para avaliar a eficiência da técnica de triagem de mutações por Polimorfismo Conformacional de Fita Simples (SSCP) por meio da comparação de resultados entre as metodologias de triagem de mutações e sequenciamento direto do gene. Foi identificada, no exon 6, uma mutação de troca de nucleotídeos em heterozigose, c.1087A>G / p.Lys363Glu, levando a produção do aminoácido ácido glutâmico ao invés da lisina em um dos pacientes avaliados. Não foram encontradas mutações em nenhum dos pacientes para os demais exons analisados. Estes resultados corroboram dados gerados por meio de SSCP, e sugerem grande eficiência da técnica de triagem para o gene LEPRE-1 para formas recessivas de Osteogênese Imperfeita
Pereira, Pedro Miguel Martins. "Inversão geoestatística de dados sísmicos 3D com multi-distribuições locais". Master's thesis, Universidade de Aveiro, 2014. http://hdl.handle.net/10773/14410.
Pełny tekst źródłaOs modelos de subsuperfície inferidos a partir de dados de reflexão sísmica permitem avaliar espacialmente as propriedades petro-elásticas de reservatório (por exemplo, densidade, impedâncias acústica e elástica) que são essenciais para uma boa caracterização e modelação de reservatórios de hidrocarbonetos. Os modelos invertidos gerados a partir de metodologias geoestatísticas e da integração de dados de logs de poços com dados de reflexão sísmica, dentro de uma grelha de reservatório, permitem ainda avaliar e quantificar, localmente, a incerteza espacial associada. O principal objetivo desta dissertação de mestrado consiste na comparação e discussão dos diversos resultados obtidos através da aplicação de diferentes metodologias de inversão sísmica geoestatística com multi-distribuições e modelos de continuidade espacial locais, de forma a gerar modelos próximos da realidade, em condições de não-estacionaridade, que permitam uma melhor caracterização da geologia de subsuperfície. Dentro das várias aproximações de inversão geoestatística existentes, para a elaboração desta tese foram utilizadas a Inversão Estocástica Global e a Inversão Elástica Global recorrendo ao algoritmo de simulação sequencial direta para a geração dos modelos elásticos de sub-superfície. Esta tese baseia-se na realização de 6 ensaios distintos, para cada uma das metodologias de inversão referidas, segundo multi-distribuições e modelos de continuidade locais (zonalidades) previamente definidas a um conjunto de dados sintéticos altamente não estacionários. No conjunto de dados disponíveis foram integrados dados de impedâncias (acústica e elástica) de 32 poços, bem como dados de reflexão sísmica post-stack. Os resultados mostram que a divisão da malha do reservatório em diferentes zonas tem um grande impacto na convergência dos diferentes métodos de inversão geoestatística utilizados no âmbito desta tese.
The subsurface models inferred from seismic reflection data allow the spatial evaluation of the petro-elastic properties of the reservoir (e.g., density, elastic and acoustic impedances) that are essential for a good characterization and modeling of hydrocarbon reservoirs. The generated inverted models from geostatistical methodologies and the integration of well log data with seismic reflection data, within a reservoir grid, allow to assess and quantify, locally, the associated spatial uncertainty. The main objective of this MSc dissertation is the comparison and discussion of the results obtained from applying different geostatistical seismic inversion methodologies, with local multi-distributions and spatial continuity models, to generate models closer to reality, under non-stationarity conditions, that allow a better characterization of the subsurface geology. From the several existing geostatistical inversion approaches, for the realization of this thesis the Global Stochastic Inversion and the Global Elastic Inversion were selected, using the direct sequential simulation algorithm to generate the subsurface elastic models. This thesis is based on the realization of 6 different trials for each of the inversion methodologies, under local multi-distributions and continuity models (zonality) predefined in a highly non-stationary synthetic dataset. In the available dataset impedance (acoustic and elastic) data from 32 wells and post-stack seismic reflection data were integrated. The results show that the division of reservoir grid into different zones has high impact on the convergence of the different inversion geostatistical methods used within this thesis.
Sukal, Amit Chand. "Molecular characterisation and diagnosis of badnaviruses infecting yams in the South Pacific". Thesis, Queensland University of Technology, 2018. https://eprints.qut.edu.au/122927/1/Amit%20Chand_Sukal_Thesis.pdf.
Pełny tekst źródłaChen, Li-Jung, i 陳麗容. "Breast cancer of Taiwan;p53 gene;SSCP;Direct DNA Sequencing". Thesis, 1995. http://ndltd.ncl.edu.tw/handle/70259252308968030663.
Pełny tekst źródła國立臺灣大學
化學研究所
84
Breast cancer is the most common malignancy cancer of women in Western countries. The mortality rate have held almost steady over the past 20 years, even though the number of new cases has grown. From 1970 to 1990, breast cancer is an important cancer of women in Taiwan and incidence of age in breast cancer trend to early onset. At molecular level, various genetic alteration have been implicated in the development of breast cancer. Loss of heterozygosity(LOH) at five different regions of human genome,including chromosome 1q, 3p, 11p, 13q and 17p, has been observed in a high percentage of primary breast cancer. Because the extremely high frequency(60%) of p53 mutation in primary breast cancer of Western countries, we will focus on mutations of the p53 tumor suppressor gene in breast cancer of Taiwan. First, we use immunohistochemical analysis to determine whether p53 gene expression in 171 breast cancer specimen of Taiwan can be detected. Second,the validity of p53 immunopositivity as an indicator for p53 mutation was verified using two molecular assays of p53 mutation: Single Strand Conformation Polymorphism(SSCP) and direct DNA sequencing. We have found p53 mutations in 26.9% of the breast cancer specimen in Taiwan. In addition, the mutation patterns of p53 gene mainly locate at exon 7∼8 and G to A (22%) transition is the major type of nucleotide changes. But in Western countries, the mutation patterns of p53 gene major locate at exon 5∼6 and C to G (20%) transversion is the major type of nucleotide changes. The spectrum of mutation in breast cancer of Taiwan differs from that of Western countries, further indicating that unknown carcinogens involve in the formation of breast cancer in Taiwan.
"Direct metagenomic detection and analysis of plant viruses using an unbiased high-throughput sequencing approach". THE UNIVERSITY OF OKLAHOMA, 2009. http://pqdtopen.proquest.com/#viewpdf?dispub=3352842.
Pełny tekst źródłaLin, Yuh-cheng, i 林育成. "Detection of p53 gene mutation in human breast neoplasm with single-strand conformation polymorphism and direct cyclic sequencing". Thesis, 1996. http://ndltd.ncl.edu.tw/handle/36528981266891361444.
Pełny tekst źródła國立成功大學
藥理學研究所
85
p53是一種癌症抑制基因(tumor supressor gene),在人類常見的癌症當 中,可發現p53基因的等位基因缺失(allelic deletion)或點突變(point mutation)所造成的功能喪失。當DNA受到了損傷之後,p53蛋白的量在細 胞內急遽增加,隨之使細胞週期停留在G1期;細胞若包含突變的p53或缺 乏p53則不能誘導此種細胞週期停止。此外,突變的p53基因使細胞進行計 畫性細胞自殺(apoptosis)的能力降低,因此癌細胞內的p53基因若突變則 其對化學療法或放射線療法將具有抵抗性。在與其他種致癌基因比較之下 ,p53基因的突變或缺失是在乳房癌中最常見的基因變化。因此,若能建 立p53基因與乳房癌惡化程度之間的關係,將有助於診斷乳房癌或者評估 其在治療後復發的可能性。本實驗的目的在於偵測不同病期的乳房癌中其 p53基因突變的情形,藉此來推斷乳房癌的預後(prognosis)。我們首先利 用去氧核醣核酸單股結構多型性(single-stand conformation polymorphism),偵測涵蓋了p53之exons 5~9的部分,篩選出具有突變的 片段,再據此進行直接循環去氧核糖核酸定序(direct-cyclic DNA sequencing)以建立p53基因在乳房癌中突變的圖譜。經篩選及定序之後, 我們發現了三個病人在exon 5的位置產生了突變。編號33的病人,其p53 基因的第175個密碼子(codon)由CGC變為TGC,即胺基酸由原本的arginine 變為cysteine。編號36的病人其p53基因第175個密碼子由原本的CGC變為 CAC,即胺基酸由正常的arginine變為histidine。編號54的病人p53基因 的第138個密碼子則由原本的GCC變為CCC,即胺基酸由正常的alanine變為 proline,而以上三者均屬於錯譯突變(missense mutation),並且其第一 個等位基因發生突變後伴隨著第二個等位基因的缺失,此現象稱之為loss of heterozygosity,是p53基因常被發現的現象之一。若想根據以上三個 p53突變之病人,來找出其病程與預後之關係,則尚無法確切地下結論。 其原因為此三人目前仍繼續在門診,其中雖有一人有癌症轉移之現象,但 代表性不夠,故仍需進一步的觀察。 p53 is a tumor supressor gene whose inactivation, either by allelic deletion or point mutation, is one of the most common genetic changes in human malignancies. The P53 protein level is strongly increased after experimental DNA damage and this is followed by a specific arrest of the cell cycle in G1 phase. Cells containing mutated p53 or lacking P53 are unable to induce this cell cycle arrest; furthermore, these cells would have a reduced capacity to induce apoptosis after DNA damage incurred by ionizing radiotherapy or some therapeutic drugs, suggesting that cancer cells containing mutated p53 are more resistant to radiotherapy or chemotherapy. Compared with the other oncogenes, p53 gene mutations and deletions are the most frequently observed genetic change in breast cancer. Therefore, p53 gene mutations coupled with the disease status could provide an invaluable tumor marker for diagnosis and/or prognosis of breast neoplasm. This research project aims at detecting p53 gene mutations from breast tumor samples of various neoplastic stages and with these information to infer the prognosis of breast cancer. We first obtained genomic DNA from archival and frozen-breast cancer tissues, then amplified exons 5,6,7,8 and 9 of p53 gene by the method of polymerase chain reaction (PCR). We used single-strand conformation polymorphism (SSCP) as a means to prescreen mutations in some specific exon fragments. Eleven variant fragments was identified by their band shifting following polyacrylamide gel electrophoresis (PAGE). Those variants are subjected to direct-cyclic sequencing so as to establish the mutation spectrum of p53 gene. Out of 11 SSCP variants, 3 showed nucleotide mutations upon sequencing. In patient number 33, codon 175 was changed from CGC to TGC, and patient number 36 has the same mutation site in codon 175: CGC ->CAC. Patient number 54 has mutation in codon 138: GCC->CCC. In agreement with other studies, these point mutations are believed to lead to changes in the conformation of p53 protein and loss of its normal functions.
Lewin, Jörn [Verfasser]. "Method development for quantitative methylation analysis by direct bisulfite sequencing, raw data processing and analysis of the Human Epigenome Project / von Jörn Lewin". 2008. http://d-nb.info/987532677/34.
Pełny tekst źródłaTESTI, Silvia. "Dissecting the genetic heterogeneity of familial dementias". Doctoral thesis, 2011. http://hdl.handle.net/11562/349107.
Pełny tekst źródłaDementia is a clinical syndrome associated with progressive deterioration of intellectual functions including loss of memory, difficulties with language, simple calculations, planning and judgment, and motor skills with eventually loss of autonomy. Currently there are an estimated 36 million people worldwide with dementia and this figure is set to increase to more than 115 million people by 2050. Given the prevalence of dementia and the associated significant financial and human costs, in recent years there has been a huge burst of studies aimed to identify the causes of this disorder and its underlain pathological mechanisms, in order to define therapeutic treatments to replace the nowadays available palliative cares. Among different subtypes of dementia, Alzheimer's Disease (AD) is the most frequent form, followed by Vascular Dementia and Frontotemporal Lobar Degeneration (FTLD) which represents the second most common form in people younger than 65 years. All three of these diseases may have a genetic component and, despite considerable progress and efforts made in recent years to clarify their molecular basis, little is known about the pathological mechanisms determining these diseases. Three forms of dementia, which may have a genetic component, were included in this study: Alzheimer's Disease, Frontotemporal Lobar Degeneration and Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leuokoencephalopathy (CADASIL) which is a subtype of vascular dementia. The main objectives of this study included mutational analysis of the genes associated with these diseases with the dual purpose of defining their mutational frequencies in an Italian series and to evaluate the presence of possible genotype-phenotype correlations. The secondary purpose is to be able to draw diagnostic algorithms useful to clinicians in selecting which patients submit to genetic testing. Two clinical cohorts were set up; one consisting in patients with diagnosis of AD, FTLD or related disorders, and the other made up of patients with CADASIL. The mutational analysis was performed by two main techniques: Denaturing High Performance Liquid Chromatography and direct sequencing. The analyzed genes encompassed: Amyloid Precursor Protein (APP), Presenilin1 (PSEN1) and Presenilin2 (PSEN2) for Alzheimer's Disease; Progranulin (PGRN), Microtubule Associated Protein Tau (MAPT), Valosin Containing Protein (VCP), Charged Multivesicular Body Protein 2B (CHMP2B), TAR DNA Binding Protein (TARDBP) for Frontotemporal Lobar Degeneration and NOTCH3 for CADASIL. The mutational analysis of AD- and FTLD-associated genes led to the identification of 8 mutations: three novel variations, PSEN1 p.Ile437Asn, PSEN2 p.Thr18Met and PGRN p.His400ThrfsX12 and five already described substitutions, PSEN2 p.Arg71Trp and p.Met174Val, PGRN p.Phe86SerfsX170 and p.Thr272SerfsX10 and MAPT c.IVS10+16C>T. The molecular data and in silico analyses performed in this study argue in favour of pathogenetic nature for Presenilins variations even though the role of some of them is debated in literature. The substitution identified in PGRN and MAPT are mutations whose pathogenic mechanism has already been described. The clinical phenotype associated to identified mutations was not unique and, especially mutations in the PGRN gene showed a marked variability in clinical presentations, even within the same family. Furthermore, the identification of a FTD-MND family linked to a locus on chromosome 9 further emphasizes the genetic heterogeneity of FTLD. The mutational screening of NOTCH3 gene led to the identification of 21 different mutations, including 7 novel variations, distributed unevenly along the gene. A geographical clustering was observed with mutations identified only in patients living in North-East Italy, a few in North-West and other in Central Italy. Haplotype analysis was performed to assess a possible founder effected underlying this regionalization but, although consistent, it was not confirmed as the majority of mutations was associated with the most common haplotype. The results of this study together were useful to define diagnostic algorithms that could help clinicians to identify patients suggestive of a molecular basis of disease to address to genetic testing.
Hanzlíčková, Johana. "Zhodnocení kryptické diverzity ve skupině lakušníku niťolistého (Ranunculus trichophyllus agg.)". Master's thesis, 2021. http://www.nusl.cz/ntk/nusl-446186.
Pełny tekst źródła"Individualizing the Informed Consent Process for Whole Genome Sequencing: A Patient Directed Approach". Doctoral diss., 2013. http://hdl.handle.net/2286/R.I.20945.
Pełny tekst źródłaDissertation/Thesis
Ph.D. Biology 2013
Jiang, Jiao. "Use of directed evolution to generate multiple-stress tolerant Oenococcus oeni for enhanced malolactic fermentation". Thesis, 2017. http://hdl.handle.net/2440/114505.
Pełny tekst źródłaThesis (Ph.D.) (Research by Publication) -- University of Adelaide, School of Agriculture, Food and Wine, 2017.
Kabahuma, Rosemary I. "Genetic aspects of hearing loss in the Limpopo Province of South Africa". Thesis, 2010. http://hdl.handle.net/10539/8566.
Pełny tekst źródłaNtuli, Zanele Nonhlanhla. "Gender and dramatic discourse with reference to Zakes Mda's selected plays". Diss., 2019. http://hdl.handle.net/10500/26234.
Pełny tekst źródłaThis dissertation examines the multiplicity of social positions within which African women in the postcolonial era find themselves. It focuses on how the dramatic dialogue depicts the positions of women in Zakes Mda’sThe Nun’s Romantic Story, And the Girls in their Sunday Dresses and You Fool, How can the Sky Fall. The study is intended to explore the dramatic dialogue in these plays and to show whether there is any evidence of change in women’s positions. It seeks to demonstrate the extent to which the positions of women have changed and also how the dramatic dialogue in the selected plays of Zakes Mda indicates the change in women’s positions.
Thutopatlisiso eno e tlhatlhoba maemo a loago a mantsintsi a basadi ba maAforika ba ba tshelang mo motlheng wa morago ga puso ya bokoloniale ba iphitlhelang ba le mo go ona. E tota ka moo puisano ya terama e bontshang maemo a basadi ka gona mo The Nun’s Romantic Story, And the Girls in their Sunday Dresses le You Fool, How can the Sky Fall tsa ga Zakes Mda. Maikaelelo a thutopatlisiso ke go sekaseka puisano ya terama mo metshamekong eno go bontsha gore a go na le bosupi bope jwa diphetogo mo maemong a basadi. E batla go bontsha ka moo maemo a basadi a fetogileng ka gona le ka moo puisano ya terama mo metshamekong e e tlhophilweng ya ga Zakes Mda e bontshang diphetogo mo maemong a basadi ka gona.
Ledisetheshini ihlolisisa tikhundlanyenti tetenhlalo bomake base-Afrika labatitfola bakuto ngemuva kwesikhatsi sembuso webukolonali (umbusobucalu). Igcile ekutsini inkhulumomphendvulwane emidlalweni yaZakes Mda itikhombisa kanjani letikhundla tabomake; i-The Nun’s Romantic Story [Indzaba yelutsandvo yemasisitela], ne-Girls in their Sunday Dresses [Emantfombatana etingutjeni tawo teLisontfo] ne-You Fool [Wena Silima], How can the Sky Fall [Singawa kanjani Sibhakabhaka]. Lolucwaningo lwentelwe kuhlolisisa inkhulumomphendvulwane kulemidlalo kanye nekukhombisa kutsi ingabe bukhona yini bufakazi bengucuko etikhundleni tabomake. Ifuna kukhombisa kutsi tikhundla tabomake tigucuke kangakanani kanye nekutsi inkhulumomphendvulwane emidlalweni lekhetsiwe yaZakes Mda ikukhombisa kanjani kugucuka kwetikhundla tabomake.
English Studies
M.A.(Theory of Literature)
Correia, João Pedro Pequito. "Contributo para a modelação 3D de horizontes geológicos com o auxílio de sísmica de reflexão e dados de sondagens e poços: um caso de estudo na Bacia Cenozóica do Baixo Tejo". Master's thesis, 2017. http://hdl.handle.net/10362/22223.
Pełny tekst źródłaMarques, Inês Alexandra Costa. "Reservatórios turbidíticos – simulação da morfologia por estatísticas multiponto e avaliação das propriedades petrofísicas". Master's thesis, 2015. http://hdl.handle.net/10362/17046.
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