Gotowe bibliografie tematyczne / Crowded lipid membrane biophysics

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Gotowa bibliografia na temat „Crowded lipid membrane biophysics”

Autor: Grafiati

Data publikacji: 6 września 2023

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Artykuły w czasopismach na temat "Crowded lipid membrane biophysics"

Erwin, Nelli, Satyajit Patra, Mridula Dwivedi, Katrin Weise i Roland Winter. "Influence of isoform-specific Ras lipidation motifs on protein partitioning and dynamics in model membrane systems of various complexity". Biological Chemistry 398, nr 5-6 (1.05.2017): 547–63. http://dx.doi.org/10.1515/hsz-2016-0289.

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Abstract The partitioning of the lipidated signaling proteins N-Ras and K-Ras4B into various membrane systems, ranging from single-component fluid bilayers, binary fluid mixtures, heterogeneous raft model membranes up to complex native-like lipid mixtures (GPMVs) in the absence and presence of integral membrane proteins have been explored in the last decade in a combined chemical-biological and biophysical approach. These studies have revealed pronounced isoform-specific differences regarding the lateral distribution in membranes and formation of protein-rich membrane domains. In this context, we will also discuss the effects of lipid head group structure and charge density on the partitioning behavior of the lipoproteins. Moreover, the dynamic properties of N-Ras and K-Ras4B have been studied in different model membrane systems and native-like crowded milieus. Addition of crowding agents such as Ficoll and its monomeric unit, sucrose, gradually favors clustering of Ras proteins in forming small oligomers in the bulk; only at very high crowder concentrations association is disfavored.

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Arnarez, C., S. J. Marrink i X. Periole. "Molecular mechanism of cardiolipin-mediated assembly of respiratory chain supercomplexes". Chemical Science 7, nr 7 (2016): 4435–43. http://dx.doi.org/10.1039/c5sc04664e.

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We reveal the molecular mechanism by which cardiolipin glues respiratory complexes into supercomplexes. This mechanism defines a new biophysico-chemical pathway of protein–lipid interplay, with broad general implications for the dynamic organization of crowded cell membranes.

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Kessler, Michael S., i Susan Gillmor. "Lipid Membrane Phase Dynamics". Biophysical Journal 104, nr 2 (styczeń 2013): 248a. http://dx.doi.org/10.1016/j.bpj.2012.11.1398.

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Nawrocki, Grzegorz, Wonpil Im, Yuji Sugita i Michael Feig. "Clustering and dynamics of crowded proteins near membranes and their influence on membrane bending". Proceedings of the National Academy of Sciences 116, nr 49 (18.11.2019): 24562–67. http://dx.doi.org/10.1073/pnas.1910771116.

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Atomistic molecular dynamics simulations of concentrated protein solutions in the presence of a phospholipid bilayer are presented to gain insights into the dynamics and interactions at the cytosol–membrane interface. The main finding is that proteins that are not known to specifically interact with membranes are preferentially excluded from the membrane, leaving a depletion zone near the membrane surface. As a consequence, effective protein concentrations increase, leading to increased protein contacts and clustering, whereas protein diffusion becomes faster near the membrane for proteins that do occasionally enter the depletion zone. Since protein–membrane contacts are infrequent and short-lived in this study, the structure of the lipid bilayer remains largely unaffected by the crowded protein solution, but when proteins do contact lipid head groups, small but statistically significant local membrane curvature is induced, on average.

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Fischer, Wolfgang B. "Assembling Within The Lipid Membrane: Viral Membrane Proteins". Biophysical Journal 96, nr 3 (luty 2009): 338a—339a. http://dx.doi.org/10.1016/j.bpj.2008.12.3823.

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Mitchison-Field, Lorna MY, i Brittany J. Belin. "Bacterial lipid biophysics and membrane organization". Current Opinion in Microbiology 74 (sierpień 2023): 102315. http://dx.doi.org/10.1016/j.mib.2023.102315.

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Ho, Chian Sing, Nawal K. Khadka, Fengyu She, Jianfeng Cai i Jianjun Pan. "Polyglutamine aggregates impair lipid membrane integrity and enhance lipid membrane rigidity". Biochimica et Biophysica Acta (BBA) - Biomembranes 1858, nr 4 (kwiecień 2016): 661–70. http://dx.doi.org/10.1016/j.bbamem.2016.01.016.

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Wang, Hongyin, Kandice R. Levental, Joseph H. Lorent, Adhvikaa A. Revathi i Ilya Levental. "Lipid scrambling facilitates membrane vesiculation through decreasing membrane stiffness". Biophysical Journal 122, nr 3 (luty 2023): 22a—23a. http://dx.doi.org/10.1016/j.bpj.2022.11.347.

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Hoopes, Matthew I., Roland Faller i Marjorie L. Longo. "Membrane Curvature Modeling and Lipid Organization in Supported Lipid Bilayers". Biophysical Journal 98, nr 3 (styczeń 2010): 78a—79a. http://dx.doi.org/10.1016/j.bpj.2009.12.445.

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Sodt, Alexander J., Olivier Soubias, Klaus Gawrisch i Richard W. Pastor. "Lipid-Lipid Coupling to Membrane Curvature by Simulation and NMR". Biophysical Journal 110, nr 3 (luty 2016): 243a. http://dx.doi.org/10.1016/j.bpj.2015.11.1340.

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Rozprawy doktorskie na temat "Crowded lipid membrane biophysics"

Botelho, Ana Vitoria. "Lipid-protein interactions: Photoreceptor membrane model". Diss., The University of Arizona, 2005. http://hdl.handle.net/10150/280765.

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G-protein coupled receptors (GPCRs) are transmembrane proteins capable of recognizing an astonishing variety of biological signals, ranging from photons of light to hormones, odorants, and neurotransmitters involved in key biological signaling processes. The aim of this work is to identify how lipid-protein interactions involving the membrane bilayer ultimately affect such vital biological functions. Here the relationship between the bilayer thickness, hydrophobic mismatch, and protein aggregation are investigated by expanding the framework of membrane-receptor interactions in terms of a new flexible surface model. Previously, we have shown how coupling of the elastic stress-strain due to mismatch of the spontaneous curvature and hydrophobic thickness at the lipid/protein interface can govern the conformational transitions of membrane proteins. This approach has now been extended to include coupling of the lateral organization of the GPCR rhodopsin to the curvature and area stress and strain of the proteolipid membrane. Rhodopsin was labeled with site-specific fluorophores, and a FRET technique was employed to probe protein association in different lipid environments. Moreover, UV-visible spectroscopy was used for thermodynamic characterization of the conformational change of rhodopsin. Lastly, the deformation of the lipids with and without rhodopsin was probed in terms of acyl chain order parameters and relaxation rates by solid-state NMR methods, giving insight into the lipid deformation. The results showed that optimal receptor activation occurs in phosphatidylcholine bilayers of 20-carbon acyl chain length, hence one can say that metarhodopsin II is likely to adopt an elongated shape. Lipids promoting aggregation, or below their gel to liquid crystalline transition temperature all favor formation of metarhodopsin I. The data also showed that association and activation of rhodopsin do not always correlate. In terms of the extended flexible surface model, the stress due to hydrophobic mismatch is coupled via the effective number of lipids surrounding the protein due to the lateral organization of the membrane. The measured changes in rhodopsin-rhodopsin interactions and membrane influences on the conformation of the protein after photoisomerization may be crucial to understanding physiological regulation of the rod disk membranes. They are relevant to understanding the complexity of biomembranes involved in many cellular mechanisms, including signal transduction.

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Liebau, Jobst. "Membrane interactions of glycosyltransferases". Licentiate thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-122485.

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Many important biological processes occur near or in membranes. The role of membranes is not merely confined to compartmentalization, they also form the matrix for membrane associated proteins and are of functional importance. Membrane associated proteins on the other hand require specific membrane properties for proper function. The interactions between membranes and proteins are thus of paramount importance and are at the focus of this work. To draw valid conclusions about the nature of such interactions the membrane mimetics required in biophysical methods must faithfully mimic crucial properties of biological membranes. To this end, new types of small isotropic bicelles which mimic plant and bacterial membranes were characterized by their size and lipid dynamics using solution-state NMR. Small isotropic bicelles are specifically well suited for solution-state NMR studies since they maintain a bilayer while being sufficiently small to conduct interpretable experiments at the same time. Monogalactosyl diacylglycerol and digalactosyl diacylglycerol, which are highly abundant in thylakoid membranes, were successfully incorporated into bicelles. Also, it was possible to make bicelles containing a lipid mixture extracted from Escherichia coli cells. A fundamental physical property of lipids in bilayers is their phase behaviour and thus the dynamics that lipids undergo in a membrane. Here, the dynamics of 13C-1H bonds in lipids were studied by nuclear spin relaxation. From such studies it was found that the glycerol backbone of lipids in bicelles is rigid while the flexibility of the acyl chain increases towards its end. Bulky head groups are rigid, while smaller head groups are more dynamic than the glycerol backbone. Acyl chain modifications, like unsaturations or cyclopropane moities, that are typically found in E. coli lipids, locally increase the rigidity of the acyl chain. Membrane interactions of a putative membrane anchor of the glycosyltransferase WaaG, MIR-WaaG, were studied by fluorescence methods, circular dichroism and solution-state NMR. It was found that MIR-WaaG binds to vesicles that mimic the anionic charge of E. coli inner membranes and that α-helical structure is induced upon interaction. The NMR-structure of MIR-WaaG agrees well with the crystal structure and from paramagnetic relaxation enhancement studies it could be concluded that a central part of MIR-WaaG is immersed in the membrane mimetic. Based on these results a model of the membrane interaction of WaaG is proposed where MIR-WaaG anchors WaaG to the cytosolic leaflet of the E. coli inner membrane via electrostatic interactions. These are potentially enhanced by membrane interactions of Tyr residues at the membrane interface and of hydrophobic residues inside the membrane.

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Al-Izzi, Sami. "Dynamics of lipid membrane tubes". Thesis, Sorbonne université, 2019. http://www.theses.fr/2019SORUS674.

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Les tubes membranaires sont des structures omniprésentes dans les cellules, et la compréhension de leur dynamique et de leur morphologie est d'une importance cruciale pour la biophysique cellulaire. Cette thèse aborde plusieurs aspects de la dynamique des tubes membranaires dans des situations où ils sont déséquilibrés par divers processus inspirés par des phénomènes biologiques. Nous analysons le gonflement de tubes due à des pompes ioniques entraînant une différence de pression osmotique, ainsi que les instabilités qui en résultent. Ceci est inspiré par la structure d'un organelle appelé le vacuole contractile, et conduit à une nouvelle instabilité avec une longueur d'onde naturelle beaucoup plus longue que celle résultant d'une instabilité de type pearling. La stabilité des tubes membranaires présentant un écoulement de cisaillement à leur surface est également analysée. Nous avons découvert et analysé une nouvelle instabilité hélicoïdale qui conduit à l’amplification des fluctuations du tube. Nous discutons de la pertinence de cette instabilité dans le processus de scission des tubes induite par la dynamine. Enfin, nous considérons la dynamique et les fluctuations d'un tube membranaire sur lequel agissent des forces actives
Membrane tubes are structures ubiquitous in cells, and understanding their dynamics and morphology is of vital importance for cellular biophysics. This thesis will discuss several aspects of the dynamics of membrane tubes in situations where they are driven out of equilibrium by various biologically inspired processes. We analyse the inflation of membrane tubes and their subsequent instability due to ion pumps driving an osmotic pressure difference. This is inspired by the structure of an organelle called the contractile vacuole complex, and leads to a new instability with a much longer natural wavelength than a typical Pearling instability. The stability of membrane tubes with a shear in the membrane flow is analysed and a novel helical instability which acts to amplify the fluctuations is found. We discuss the relevance of this instability in the process of Dynamin mediated tube scission. Finally we consider the dynamics and fluctuations of a membrane tube with active forces acting on it

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Unnerståle, Sofia. "NMR Investigations of Peptide-Membrane Interactions, Modulation of Peptide-Lipid Interaction as a Switch in Signaling across the Lipid Bilayer". Licentiate thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-59534.

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The complexity of multi cellular organisms demands systems that facilitate communicationbetween cells. The neurons in our brains for instance are specialized in this cell-cellcommunication. The flow of ions, through their different ion channels, across the membrane, isresponsible for almost all of the communication between neurons in the brain by changing theneurons membrane potentials. Voltage-gated ion channels open when a certain thresholdpotential is reached. This change in membrane potential is detected by voltage-sensors in the ionchannels. In this licentiate thesis the Homo sapiens voltage- and calcium-gated BK potassiumchannel (HsapBK) has been studied. The NMR solution structure of the voltage-sensor ofHsapBK was solved to shed light upon the voltage-gating in these channels. Structures of othervoltage-gated potassium channels (Kv) have been determined by other groups, enablingcomparison among different types of Kv channels. Interestingly, the peptide-lipid interactions ofthe voltage-sensor in HsapBK are crucial for its mechanism of action.Uni cellular organisms need to sense their environment too, to be able to move towardsmore favorable areas and from less favorable ones, and to adapt their gene profiles to currentcircumstances. This is accomplished by the two-component system, comprising a sensor proteinand a response regulator. The sensor protein transfers signals across the membrane to thecytoplasm. Many sensor proteins contain a HAMP domain close to the membrane that isinvolved in transmitting the signal. The mechanism of this transfer is not yet revealed. Ourstudies show that HAMP domains can be divided into two groups based on the membraneinteraction of their AS1 segments. Further, these two groups are suggested to work by differentmechanisms; one membrane-dependent and one membrane-independent mechanism.Both the voltage-gating mechanism and the signal transduction carried out by HAMPdomains in the membrane-dependent group, demand peptide-lipid interactions that can be readilymodulated. This modulation enables movement of peptides within membranes or within thelipid-water interface. These conditions make these peptides especially suitable for NMR studies.

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Danial, John Shokri Hanna. "Imaging lipid phase separation in droplet interface bilayers". Thesis, University of Oxford, 2015. https://ora.ox.ac.uk/objects/uuid:34bb015f-2bc1-43bb-bc29-850e0b55edac.

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The spatiotemporal organization of membrane proteins is implicated in cellular trafficking, signalling and reception. It was proposed that biological membranes partition into lipid rafts that can promote and control the organization of membrane proteins to localize the mentioned processes. Lipid rafts are thought to be transient (microseconds) and small (nanometers), rendering their detection a challenging task. To circumvent this problem, multi-component artificial membrane systems are deployed to study the segregation of lipids at longer time and length scales. In this thesis, multi-component Droplet Interface Bilayers (DIBs) were imaged using fluorescence and interferometric scattering microscopy. DIBs were used to examine and manipulate microscopic lipid domains and to observe, for the first time, transient nanoscopic lipid domains. The techniques and results described here will have important implications on future research in this field.

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Kohram, Maryam. "A Combined Microscopy and Spectroscopy Approach to Study Membrane Biophysics". University of Akron / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=akron1436530389.

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Xu, Yuanda. "Thermodynamic and Hydrodynamic Coupling Effects on Compositional Lipid Domains in Membrane Stack Systems". Thesis, Princeton University, 2018. http://pqdtopen.proquest.com/#viewpdf?dispub=10642189.

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This dissertation will focus on my work in biophysics, and my work in mean field games and glucose predictive analysis will not be presented. Several problems relating to the effects of thermodynamic coupling and hydrodynamic coupling within the membrane stack system are discussed. Three theoretical approaches are employed and proposed to study the membrane stack system: a diffuse-interface approach is utilized for numerical simulations; a coarse-grained sharp-interface approach is utilized to provide physical understanding of various kinetics; a hybrid intermediate sharp-interface approach is adopted to study the domain coalescence in the absence of diffusion.

In the first part of the thesis, we discuss the thermodynamic coupling in membrane stack systems. Comprehensive analyses are presented to understand the accelerated coarsening kinetics with respect to single layer and long-range alignment. Numerical simulations are conducted for three systems, namely a diffusion dominated system, an advective interlayer friction dominated system, and an advective membrane viscosity dominated system. Experimental results regarding the advective interlayer friction dominated system are supported by simulations. We investigate the mechanism of the enhanced coarsening kinetics in membrane stack systems and the relationship between the coarsening process and vertical alignment. An intuitive understanding along with analytical explanations are further presented. Moreover, numerical results regarding the critical mixture are also discussed.

We then investigate the interfacial fluctuation behavior within membrane stack systems. The hydrodynamic coupling is found to play a significant role and several physical length scales are found to be crucial. Both a sharp-interface approach and a diffuse-interface approach are employed to numerically simulate decay of interface fluctuations in representative two-membrane systems.

To measure the thermodynamic coupling in experiments, the hydrodynamic force needs to be quantified, especially for the non-circular domains. In the last part of this thesis, the drag coefficient relating domain velocity and force acting on the domain is calculated using perturbation theory within two limits: the first limit refers to a domain much larger than the hydrodynamic screening length; the second limit refers to a domain that is much smaller than the hydrodynamic screening length.

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Göpfrich, Kerstin. "Rational design of DNA-based lipid membrane pores". Thesis, University of Cambridge, 2017. https://www.repository.cam.ac.uk/handle/1810/269318.

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DNA nanotechnology has revolutionised our capability to shape and control three-dimensional structures at sub-nanometre length scales. In this thesis, we use DNA to build synthetic membrane-inserting channels. Porphyrin and cholesterol tags serve as membrane anchors to facilitate insertion into the lipid membrane. With atomic force microscopy, confocal imaging and ionic current recordings we characterise our DNA nanochannels that mimic their natural protein-based counterparts in form and function. We find that they exhibit voltage-dependent conductance states. Amongst other architectures, we create the largest man-made pore in a lipid membrane to date approaching the electrical diameter of the nuclear pore complex. Pushing the boundaries on the other end of the spectrum, we demonstrate the ultimately smallest DNA membrane pore made from a single membrane-spanning DNA duplex. Thereby, we proof that ion conduction across lipid membranes does not always require a physical channel. With experiments and MD simulations we show that ions flow through a toroidal pore emerging at the DNA-lipid interface around the duplex. Our DNA pores spanning two orders of magnitude in conductance and molecular weight showcase the rational design of synthetic channels inspired by the diversity of nature - from ion channels to porins.

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Rieth, Monica D. "Investigating Detergent and Lipid Systems for the Study of Membrane Protein Interactions| Characterizing Caveolin Oligomerization". Thesis, Lehigh University, 2014. http://pqdtopen.proquest.com/#viewpdf?dispub=3638680.

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Membrane proteins represent an important class of proteins that closely associate or reside within the plasma membrane of the cell. They play a multitude of roles in cell function such as signaling, trafficking, and recently discovered, scaffolding and shaping of the plasma membrane itself. For example, caveolin is a membrane protein that is believed to have the ability to curve the plasma membrane forming invaginations that serve as signaling platforms called caveolae. The curvature of the plasma membrane is believed to be a result of caveolin oligomerization. Caveolin oligomerization was characterized using sedimentation equilibrium analytical ultracentrifugation. Due to the extremely hydrophobic nature of caveolin it was necessary to explore different detergents and lipid systems that support membrane protein structure and function. Not all detergents are conducive to studies of membrane proteins and it is often necessary to determine empirically the best detergent / lipid mimic best suited for biophysical studies. One membrane mimic that has been well-characterized and used successfully to study membrane proteins are bicelles. Bicelles are discoidal phospholipid structures comprised of a long-chain and short-chain phospholipid, typically 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dihexanoyl- sn-glycero-3-phosphocholine (DHPC), respectively. Bicelles provide a true bilayer environment in which to study membrane protein structure and function. These lipid structures were successfully density matched using the method of sedimentation equilibrium in the analytical ultracentrifuge by adding 71.7% D₂O as a density modifier. We explored the utility of bicelles as a medium for studying membrane protein interactions in the analytical ultracentrifuge (AUC) by investigating the interactions of caveolin-1. The results of this work show that caveolin-1 does not have the capacity to oligomerize in detergent micelles or in a bilayer environment (bicelles). On the other hand, a naturally-occuring breast cancer mutant, P132L, forms a strong dimer in detergent micelles. A close investigation of the mutant reveals that an extension of helix 2 in the intramembrane region of the protein where dimerization was shown to occur may play a key role in the dimerization of the mutant.

An alternative bicelle system was also investigated using pentaethylene glycol monooctyl ether (C₈E₅) instead of DHPC to form the rim of the bicelle. The C₈E₅ / DMPC lipid aggregates were density matched and their properties were characterized using ³¹P-phosphorus NMR to assess the heterogeneity of the lipid / detergent arrangement, which confirms a bicellar-like arrangement. C₈E₅ has a density similar to water (1.007 g / mL) and was shown to form lipid aggregate structures with DMPC that are less dense and require significantly lower quantity of D₂O to density match in the AUC making them better suited to the study of membrane protein interactions of small peptides.

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Köcher, Paul Tilman. "Nanoscale measurements of the mechanical properties of lipid bilayers". Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:0b478b9f-70fc-436f-9803-5d3a203f0d7e.

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Lipid bilayers form the basis of the membranes that serve as a barrier between a cell and its physiological environment. Their physical properties make them ideally suited for this role: they are extremely soft with respect to bending but essentially incompressible under lateral tension, and they are quite permeable to water but essentially impermeable to ions which allows the rapid establishment of the osmotic gradients. The function of membrane proteins, which are vital for tasks ranging from signal transduction to energy conversion, depends on their interactions with the lipid environment. Because of the complexity of natural membranes, model systems consisting of simpler lipid mixtures have become indispensable tools in the study of membrane biophysics. The objective of the work reported here is to develop a deeper understanding of the underlying physics of lipid bilayers through nanoscale measurements of the mechanical properties of mixed lipid systems including cholesterol, a key ingredient of cell membranes. Atomic force microscopy (AFM) has been used extensively to measure the topographical and elastic properties of supported lipid bilayers displaying complex phase behaviour and containing mixtures of important PC, PE lipids and cholesterol. Phase transformations have been investigated varying the membrane temperature, and the effects of cholesterol in controlling membrane fluidity, phase, and energetics have been studied. Elastic modulus measurements were correlated with phase behaviour observations. To aid in the nanoscale probing of lipid bilayers, AFM probes with a high aspect ratio and tip radii of $sim$4~nm were fabricated and characterised. These probes were used to investigate the phase boundary in binary and ternary lipid systems, leading to the discovery of a raised region at the boundary which has implications for the localisation of reconstituted proteins as well as the role of natural domains or lipid rafts. The electrical properties of the probes were examined to assess their potential application for combined structural and electrical measurements in liquid. A novel technique was developed to aid in the study of the physical properties of lipid bilayers. Membrane budding was induced above microfabricated substrates through osmotic pressure. Modification of the adhesion energy of the bilayer through biotin-avidin linking was successful in modulating budding behaviour of liquid disordered bilayers. The free energy of the system was modelled to allow quantitative information to be extracted from the data.

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Książki na temat "Crowded lipid membrane biophysics"

J, Fielding Christopher, red. Lipid rafts and caveolae: From membrane biophysics to cell biology. Weinheim: Wiley-VCH, 2006.

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Hianik, Tibor. Bilayer lipid membranes: Structure and mechanical properties. Dordrecht: Kluwer Academic Publishers, 1995.

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1958-, Gutberlet T., i Katsaras J. 1958-, red. Lipid bilayers: Structure and interactions. Berlin: Springer, 2001.

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Tien, H. TI, i Angelica Ottova-Leitmannova. Membrane Biophysics (Membrane Science and Technology). Elsevier Science, 2000.

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Membrane Biophysics - Planar Lipid Bilayers and Spherical Liposomes. Elsevier, 2000. http://dx.doi.org/10.1016/s0927-5193(00)x8022-9.

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Chemistry, Royal Society of. Lipids and Membrane Biophysics: Faraday Discussion 161. Royal Society of Chemistry, The, 2013.

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Ottova-Leitmannova, A., i H. T. Tien. Membrane Biophysics: As Viewed from Experimental Bilayer Lipid Membranes. Elsevier Science & Technology Books, 2000.

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Lipid rafts and caveolae: From membrane biophysics to cell biology. Weinheim, DE: Wiley-VCH, 2007.

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Fielding, Christopher J. Lipid Rafts and Caveolae: From Membrane Biophysics to Cell Biology. Wiley & Sons, Incorporated, John, 2006.

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Fielding, Christopher J. Lipid Rafts and Caveolae: From Membrane Biophysics to Cell Biology. Wiley-VCH Verlag GmbH, 2006.

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Części książek na temat "Crowded lipid membrane biophysics"

Bozelli, José Carlos, i Richard M. Epand. "Membrane Lipid Domains". W Encyclopedia of Biophysics, 1–11. Berlin, Heidelberg: Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/978-3-642-35943-9_547-1.

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Cevc, Gregor. "Lipid Membrane Electrostatics". W Encyclopedia of Biophysics, 1–9. Berlin, Heidelberg: Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/978-3-642-35943-9_595-1.

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Cevc, Gregor. "Membrane Lipid Electrostatics". W Encyclopedia of Biophysics, 1446–52. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-16712-6_595.

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Zha, Jialu, i Dianfan Li. "Lipid Cubic Phase for Membrane Protein X-ray Crystallography". W Membrane Biophysics, 175–220. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-6823-2_7.

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Brown, Michael F., Udeep Chawla i Suchithranga M. D. C. Perera. "Membrane Lipid-Protein Interactions". W Springer Series in Biophysics, 61–84. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-6244-5_3.

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Sharma, Charul, Priya Vrat Arya i Sohini Singh. "Lipid and Membrane Structures". W Introduction to Biomolecular Structure and Biophysics, 139–82. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-4968-2_6.

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Conn, Charlotte E. "Lipid Mesophases for Crystallizing Membrane Proteins". W Encyclopedia of Biophysics, 1269–73. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-16712-6_568.

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Watts, Anthony. "Protein-lipid interactions at membrane surfaces". W Springer Series in Biophysics, 23–50. Berlin, Heidelberg: Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-74471-6_3.

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Léonard, Catherine, David Alsteens, Andra C. Dumitru, Marie-Paule Mingeot-Leclercq i Donatienne Tyteca. "Lipid Domains and Membrane (Re)Shaping: From Biophysics to Biology". W Springer Series in Biophysics, 121–75. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-6244-5_5.

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Caffrey, Martin. "Structure and Dynamic Properties of Membrane Lipid and Protein". W Electrostatic Effects in Soft Matter and Biophysics, 1–26. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-010-0577-7_1.

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Streszczenia konferencji na temat "Crowded lipid membrane biophysics"

Jiang, Yanfei, Guy M. Genin, Srikanth Singamaneni i Elliot L. Elson. "Interfacial Phases on Giant Unilamellar Vesicles". W ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80942.

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Lipid nanodomains in cell membranes are believed to play a significant role in a number of critical cellular processes (Elson, et al., 2010). These include, for example, replication processes in enveloped viruses such as bird flu and HIV and signaling mechanisms underlying pathological conditions such as cancer. Due to the potential for developing new disease treatments through the control of these membrane rafts, the biophysics underlying their formation has been the subject of intense study, much of this focused on domain formation in giant unilamellar lipid vesicles (GUVs), a simplified model system.

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Huang, Yong, i Boris Rubinsky. "A Microfabricated Chip for the Study of Cell Electroporation". W ASME 2000 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2000. http://dx.doi.org/10.1115/imece2000-2233.

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Abstract It has been observed that when certain electrical potentials are applied across a cell they can induce the formation of pores in the cell membrane and consequently increase the permeability of the cell to macromolecules. This phenomenon is known as electroporation. Since the first report on gene transfer by electroporation1, it has become a standard method for introduction of macromolecules into cells2 3 4. Currently, electroporation is normally done in batches of cells between electrodes and there is little control over the permeabilization of individual cells. Therefore, it is very difficult to study the fundamental biophysics of cell membrane electro-permeabilization and to design optimal electroporation protocols for individual cells2 3 . Although the biophysics of electroporation are still not fully understood, indirect evidence shows that micro aqueous pores with diameters of tens to hundreds of angstroms are created in the cell membrane due to the electrical field induced structural rearrangement of the lipid bilayer5. It occurred to us that if electroporation induces pores in the cell membrane, then in a state of electroporation, a measurable current should flow through the individual cell. From this idea, we have developed a new micro-electroporation technology that employs a “bionic” chip to study and control the electroporation process in individual cells. The micro-electroporation chip, shown schematically in Figure 1, is designed and fabricated using standard silicon microfabrication technology. Each chip is a three-layer device that consists of two translucent poly silicon electrodes and a silicon nitride membrane, which all together form two fluid chambers. The two chambers are interconnected only through a micro hole through the dielectric silicon nitride membrane. In a typical process, the two chambers are filled with conductive solutions and one chamber contains biological cells. Individual cells can be captured in the micro hole and thus incorporated into the electrical circuit between the two electrodes of the chip. When the cell is in its normal state no current flows through the insulating lipid bilayer and consequently between the electrodes. However, when the electrical potential across the electrodes is sufficient to induce electroporation, a measurable current will flow through the pores of the cell membrane and between the electrodes. Measuring the currents through the bionic chip in real time will reveal the information of the state of electropermeabilization in cell membrane. The breakdown potential of irreversible electroporation, the most critical parameter in electroporation process, can be detected by analyzing current signals as well. Figure 2 illustrates a typical electrical signature in an irreversible electroporation process. Once the target cell is electroporated by the application of sufficient electroporation electrical potentials, macromolecules that are normally impermeant to cell membrane can be uploaded into the cell. Figure 3 shows how a cell entrapped in a hole is loaded during electroporation with a fluorescent die. With the ability to manipulate individual cells and detect the electrical potentials that induce electroporation in each cell, the chip can be used to study the fundamental biophysics of membrane electropermeabilization on the single cell level and in biotechnology, for controlled introduction of macromolecules, such as DNA fragments, into individual cells. We anticipate that this new technology will change the way in which electroporation is done and will provide key understanding of the biophysical processes that lead to cell electroporation. This paper will discuss the design, fabrication of the micro-electroporation chip, the experiment system as well as experiments carried out to precisely detect the parameters of electroporation of individual biological cells.

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Abstract It has been observed that when certain electrical potentials are applied across a cell they can induce the formation of pores in the cell membrane and consequently increase the permeability of the cell to macromolecules. This phenomenon is known as electroporation. Since the first report on gene transfer by electroporation1, it has become a standard method for introduction of macromolecules into cells2 3 4. Currently, electroporation is normally done in batches of cells between electrodes and there is little control over the permeabilization of individual cells. Therefore, it is very difficult to study the fundamental biophysics of cell membrane electro-permeabilization, which is not yet understood, and to design optimal and reversible electroporation protocols for individual cells2 3. Although the biophysics of electroporation are still not fully understood, indirect evidence shows that micro aqueous pores with diameters of tens to hundreds of angstroms are created in cell membrane due to the electrical field induced structural rearrangement of the lipid bilayer5. It occurred to us that if electroporation induces pores in the cell membrane than, in a state of electroporation, a measurable current should flow through the individual cell. From this idea, we have developed a new micro-electroporation technology that employs a “bionic” chip to study and control the electroporation process in individual cells. The micro-electroporation chips are designed and fabricated using standard silicon microfabrication technology. Figure 1 shows the schematic of the chip in cross section. Each chip is a three-layer device that consists of two translucent poly silicon electrodes and a silicon nitride membrane, which all together form two fluid chambers. The two chambers are interconnected only through a micro hole on the dielectric silicon nitride membrane. In a typical process, the two chambers are filled with conductive solutions and one chamber contains biological cells. Individual cells can be captured in the micro hole and thus incorporated in the electrical circuit between the two electrodes of the chip. When the cell is in its normal state no current flows through the insulating lipid bilayer and consequently between the electrodes. However, when the electrical potential across the electrodes is sufficient to induce electroporation, a measurable current will flow through the pores of the cell membrane and between the electrodes. Measuring currents through the bionic chip as a function of electrical potential will determine the potential that induces the electroporation. The chip behaves somewhat similarly to an electrical diode, with no current at potentials that do not induce electroporation and currents at potentials that induce electroporation. With the ability to manipulate individual cells and detect the electrical potentials that induce electroporation in each cell, the chip can be used to study the fundamental biophysics of membrane electro-permeabilization on single cell level and in biotechnology, for controlled introduction of macromolecules, such as gene constructs, into individual cells. We anticipate that this new technology will change the way in which electroporation is done and will provide key understanding of the biophysical processes that lead to cell electroporation. In this paper, first the design, fabrication process and modeling of the microelectroporation chip are described in details. Subsequently, experiment methods and results are presented and discussed, demonstrating the feasibility of altering cell membrane permeability and facilitating intercellular mass transfer in a more controlled way on single cell level. Finally, the potential applications of the micro-electroporation chips and future research directions are discussed. Figure 2 demonstrates how cell membrane electroporation can be investigated through monitoring and analyzing chip current-voltage signatures.

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