Artykuły w czasopismach na temat „Crolla family”

Giacinto Scelsi, last Count of Dayala Valva and one of the most extraordinary composers of this century, died in Rome on 9 August 1988 at the age of 85 in the Policlinico Gemelli after an attack brought on by the sweltering Roman summer: he who never went to the mountains to avoid it, thinking that warmth could do him only good. After the Naples earthquake of 1980, which flattened the mediaeval hill town of Valva and with it the family castle and its library, Scelsi said: crolla il castello, crolla il padrone. The castle falls to bits and so does its master. Those of us who knew him in his last years remember above all the frail figure sitting on a couch below the two portraits that Dali had given the Eluards for a wedding present, doing ironic and at times testy battle with the world and old age, there in his overheated house across from the Roman Forum. With such a view, he used to say, what one does must be quite splendid or else a very bad joke. During his lifetime Scelsi refused to be photographed, did his best to avoid programme notes, and gave information about his life only when he chose to forget himself in conversation. Few of us cared to violate these rules, knowing that for a man who had dictated the mémoires of his future life they represented a kind of defence against a finality imposed from without. He sought something like this in his music as well, hoping it would seem only a snatch of what had been going on long before, of what would be going on long after.

Purpose – to present contextual factors of destination country and discuss about their role and potential impact for family reunification and family migrants’ social adaptation. Design/methodology/approach – secondary data analysis and qualitative research. Findings – Sociological analysis of ethnic minorities’ and migrants’ social adaptation suggests taking into account the relevance of social context of the receiving society. In order to explain the concept of social context, it is suggested to apply segmented assimilation theory. According to the theory social context of the receiving society consists of three items: migration and migrants’ integration policies implemented by the host country’s government; receiving society’s attitudes and prejudices about immigrants; co- ethnic communities of immigrants and their resources to support newcomers. This paper discusses about the role and potential impact of the first item – migration and migrants’ integration policy for family migrants’ social adaptation. Migration policy is one of the most important contextual factors as it is the first one that migrants face and continually have to deal with their entire life in the destination country. Empirical data of the research, which aim was to reveal patterns of social adaptation of persons reunified with their family members in Lithuania (family migrants), confirms that social context of the host country has an impact for migrants’ social adaptation into receiving society. The analysis of national policies in the context of family reunification and secondary data analysis of the study MIPEX suggest that conditions for family reunification in Lithuania are ‘halfway favourable’, but the status of family migrants is extremely vulnerable. Migration policy and its measures applied for family reunification in Lithuania can be ascribed to the governmental response of ‘passive acceptance’ – there is a possibility to reunify with the family in Lithuania, but the process to receive residence permit is very bureaucratic and long-lasting, and, consequently, limiting migrants’ social adaptation. Research limitations/implications – This study broadens the knowledge of the phenomenon of family reunification in Lithuania and suggests a theoretical insight to study social adaptation of family migrants taking into account the impact of contextual factors of the receiving society by applying the segmented assimilation theory. Originality/Value – The study focuses on the phenomenon of family reunification, which is methodologically and empirically, marginalized in the context of migration research both on national and international level. Besides, it suggests a theoretical insight to study family migrants’ social adaptation taking into account the impact of contextual factors of the receiving society by applying segmented assimilation theory. Keywords: immigration; family reunification, social adaptation, contextual factors, migration policy. Research type: research paper JEL classification: J6 - Mobility, Unemployment, Vacancies, and Immigrant Workers K37 - Immigration Law

The boring giant clam Tridacna crocea is an evolutionary, ecologically, economically, and culturally important reef-dwelling bivalve targeted by a profitable ornamental fishery in the Indo-Pacific Ocean. In this study, we developed genomic resources for T. crocea. Using low-pass (=low-coverage, ~6×) short read sequencing, this study, for the first time, estimated the genome size, unique genome content, and nuclear repetitive elements, including the 45S rRNA DNA operon, in T. crocea. Furthermore, we tested if the mitochondrial genome can be assembled from RNA sequencing data. The haploid genome size estimated using a k-mer strategy was 1.31–1.39 Gbp, which is well within the range reported before for other members of the family Cardiidae. Unique genome content estimates using different k-mers indicated that nearly a third and probably at least 50% of the genome of T. crocea was composed of repetitive elements. A large portion of repetitive sequences could not be assigned to known repeat element families. Taking into consideration only annotated repetitive elements, the most common were classified as Satellite DNA which were more common than Class I-LINE and Class I-LTR Ty3-gypsy retrotransposon elements. The nuclear ribosomal operon in T. crocea was partially assembled into two contigs, one encoding the complete ssrDNA and 5.8S rDNA unit and a second comprising a partial lsrDNA. A nearly complete mitochondrial genome (92%) was assembled from RNA-seq. These newly developed genomic resources are highly relevant for improving our understanding of the biology of T. crocea and for the development of conservation plans and the fisheries management of this iconic reef-dwelling invertebrate.

Fishes live in aquatic environments and several aquatic environmental factors have undergone recent alterations. The molecular mechanisms underlying fish responses to hypoxia and acidification stress have become a serious concern in recent years. This study revealed that hypoxia and acidification stress suppressed the growth of body length and height of the large yellow croaker (Larimichthys crocea). Subsequent transcriptome analyses of L. crocea juveniles under hypoxia, acidification, and hypoxia–acidification stress led to the identification of 5897 differentially expressed genes (DEGs) in the five groups. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that several DEGs were enriched in the ‘protein digestion and absorption’ pathway. Enrichment analysis revealed that this pathway was closely related to hypoxia and acidification stress in the five groups, and we found that genes of the collagen family may play a key role in this pathway. The zf-C2H2 transcription factor may play an important role in the hypoxia and acidification stress response, and novel genes were additionally identified. The results provide new clues for further research on the molecular mechanisms underlying hypoxia–acidification tolerance in L. crocea and provides a basic understanding of the potential combined effects of reduced pH and dissolved oxygen on Sciaenidae fishes.

Leaf chlorosis, severe defoliation and wilt associated with root rot were observed on mature olive trees cv. Nera di Gonnos in an experimental orchard at Mirto Crosia (Calabria, southern Italy). An oomycete was consistently isolated from rotten roots of symptomatic olive trees. It was identified as Phytophthora bilorbang by morphological characters and sequencing of Internal Transcribed Spacer (ITS) regions of ribosomal DNA (rDNA). Pathogenicity was verified by inoculating potted two-month-old rooted cuttings of Olea europaea var. Nera di Gonnos in a soil infestation trial. P. bilorbang was re-isolated from roots of symptomatic, artificially inoculated olive cuttings to fulfill Koch’s postulates. This is the first report of P. bilorbang on O. europaea L. and on a species of the Oleaceae family worldwide.

Uncertainty and controversy exist in the phylogenetic status of the Sciaenidae family because of the limited genetic data availability. In this study, a data set of 69,098 bp, covering 309 shared orthologous genes, was extracted from 18 genomes and 5 transcriptomes of 12 species belonging to the Sciaenidae family and used for phylogenetic analysis. The maximum likelihood (ML) and Bayesian approach (BA) methods were used to reconstruct the phylogenetic trees. The resolved ML and BA trees showed similar topology, thus revealing two major evolutionary lineages within the Sciaenidae family, namely, Western Atlantic (WA) and Eastern Atlantic–Indo–West Pacific (EIP). The WA group included four species belonging to four genera: Cynoscion nebulosus, Equetus punctatus, Sciaenops ocellatus, and Micropogonias undulatus. Meanwhile, the EIP group formed one monophyletic clade, harboring eight species (Argyrosomus regius, A. japonicus, Pennahia anea, Nibea albiflora, Miichthys miiuy, Collichthys lucidus, Larimichthys polyactis, and L. crocea) from six genera. Our results indicated that the Western Atlantic (WA) group was more ancient in the studied species, while the Eastern Atlantic–Indo–West Pacific (EIP) group was a younger group. Within the studied species, the genera Collichthys and Larmichthys were the youngest lineages, and we do not suggest that Collichthys and Larmichthys should be considered as one genus. However, the origin of the Sciaenidae family and problems concerning the basal genus were not resolved because of the lack of genomes. Therefore, further sampling and sequencing efforts are needed.

Heat shock proteins 70 (Hsp70) are required for key cellular processes and responses to environmental changes, however, there are an unknown number of hsp70 gene family members in the large yellow croaker (Larimichthys crocea). In the present study, 17 hsp70 genes were identified through the genome of the large yellow croaker. These genes are divided into seven evolutionarily distinct groups according to a phylogenetic tree. The orthologs of these hsp70 genes were found in humans and zebrafish. The expression patterns of the hsp70 gene family in the large yellow croaker under cold and heat stress were studied by examining transcriptome data. Six out of 17 genes were significantly unregulated or downregulated after cold or heat stress. There were two genes significantly upregulated and two genes downregulated in the liver after cold treatment, while after heat treatment, five genes were significantly upregulated, and no genes were significantly downregulated. Three expression patterns were detected: strictly heat-inducible hsp70, constitutively expressed and moderately heat-inducible hsp70, and constitutively expressed and less stress-dependent hsp70 genes. All the findings will contribute to a better understanding of the biological function of hsp70s in defending against thermal challenges.

In this study, we designate lectotypes and fix the identities of two Australian Castniidae species, Synemon sophia (White, 1841), the type species of Synemon Doubleday, 1846, and Synemon parthenoides R. Felder, 1874. Furthermore, we describe seven new species of Castniidae from Western Australia, two new species of the Synemon sophia group, Synemon anthracica Kallies & Edwards sp. n. and Synemon crocea Kallies & Edwards sp. n., three species in the Synemon magnifica Strand, 1911 group, Synemon semaphora Kallies & Edwards sp. n., Synemon angustiptera Kallies & Edwards sp. n. and Synemon petrophila Kallies & Edwards sp. n., one new species of unclear affinity, Synemon cacumina Kallies & Edwards sp. n., and one new species related to Synemon directa Westwood, 1877, Synemon victoriae Kallies & Edwards sp. n. We report Restionaceae, a host-plant family not previously recorded for Castniidae.

Ocean ecosystems are highly effective in the recycling of energy and matter. Carbon fixation is almost recycled because net carbon burial in terrestrial systems and export to the ocean via rivers. Heterotrophs efficiently reprocess organic matter because they depend on the energy in organic matter. Withal, heterotrophs cannot use total organic energy because some is shunted into metabolites like ammonium, and under anoxic conditions into reduced substances such as sulphide. These reduced inorganic compounds are used by chemo (litho) autotrophs to obtain energy for inorganic carbon fixation. Host - associated microbial symbionts are critical to the conversion of inorganic carbon into organic biomass (Beinart, R.A., 2019). In the world’s oceans, Boring clams belongs to family Teredinidae, (Shipworms) with habitat of eating wood, assisted by cellulases from the intracellular symbiotic gammaproteobacteria that inhabit their gills. Other shipworms (Kuphus polythalamius) also relying on gill-dwelling gammaproteobacteria for sulphur oxidation (Altamia et al., 2020) and Methane Oxidation. The Symbionts of the gills Teredinibacter turnerae T7901 and similar strains are among the greatest sources of Biosynthetic Gene Clusters (BGCs), with content equivalent to well-known commercial manufacturers such as Streptomyces spp. This implies that shipworms might be a good source of new compounds for drug discovery (Altamia et al., 2020).

Seven genera new to science, i.e.: Helmutiopsis, Huriopsis, Johnsheardia, Klauskalbia, Kudratovia, Kurokawia and Poeltonia of the Physciaceae are proposed for the ‘Rinodina’ atrocinerea, the ‘Rinodina’ xanthophaea, the ‘Rinodina’ cinnamomea, the ‘Heterodermia’ obscurata, the ‘Rinodina’ straussii, the ‘Anaptychia’ isidiata and the ‘Physconia’ grisea groups consequently that all form strongly supported monophyletic branches in a phylogeny analysis based on a combined matrix of nrITS and mtSSU sequences. Phylogenetic positions of species belonging to the genera Kashiwadia s. l., Leucodermia, Mischoblastia,Oxnerella, Phaeorrhiza s. l., Polyblastidium and Rinodinella s. l. are discussed. Oxnerella afghanica which for the first time recorded as parasitic lichen species from both epiphytic and saxicolous crustose lichens is designated as type species for the genus Oxnerella. Sequences of the recently described Physcia orientostellaris as well as Huriopsis xanthophaea and additional sequences of Kashiwadia aff. orientalis and Mischoblastia aff. oxydata are submitted to the GenBank. The positions of Polyblastidium casaterrinum from Costa Rica, ‘Rinodina’ efflorescens from Białowieża, Poland, and ‘Mischoblastia’ confragosula from Cambodia in the Physciaceae are confirmed in a phylogeny analysis based on the nrITS sequences. The presence of ‘extraneous mycobiont DNA’ in lichen associations is exemplified with earlier incorrect identifications of Heterodermia, Kashiwadia, Kurokawia,Oxnerella and Poeltonia specimens. Fifty-six new combinations are presented: Helmutiopsis alba (for Rinodina alba Metzler ex Arn.), Helmutiopsis aspersa (for Lecanora aspersa Borrer), Helmutiopsis atrocinerea (for Parmelia atrocinerea Fr.), Huriopsis chrysidiata (for Rinodina chrysidiata Sheard), Huriopsis chrysomelaena (for Rinodina chrysomelaena Tuck.), Huriopsis lepida (for Lecanora lepida Nyl.), Huriopsis luteonigra (for Rinodina luteonigra Zahlbr.), Huriopsis plana (for Rinodina plana H. Magn.), Huriopsis thiomela (for Lecanora thiomela Nyl.), Huriopsis xanthomelana (for Rinodina xanthomelana Müll. Arg.), Huriopsis xanthophaea (for Lecanora xanthophaea Nyl.), Johnsheardia cinnamomea (for Rinodina mniaroea var. cinnamomea Th. Fr.), Johnsheardia herteliana (for Rinodina herteliana Kaschik), Johnsheardia jamesii (for Rinodina jamesii H. Mayrhofer), Johnsheardia reagens (for Rinodina reagens Matzer et H. Mayrhofer), Johnsheardia zwackhiana (for Lecanora zwackhiana Kremp.), Kashiwadia austrostellaris (for Physcia austrostellaris Elix), Kashiwadia jackii (for Physcia jackii Moberg), Kashiwadia littoralis for Physcia littoralis Elix), Kashiwadia nubila (for Physcia nubila Moberg), and Kashiwadia tropica (for Physcia tropica Elix), Klauskalbia crocea (for Heterodermia crocea R. C. Harris), Klauskalbia flabellata (for Parmelia flabellata Fée), Klauskalbia obscurata (for Physcia speciosa (Wulfen) Nyl. *obscurata Nyl.), Klauskalbia paradoxa (for Heterodermia paradoxa Schumm et Schäfer-Verwimp), Kudratovia bohlinii (for Rinodina bohlinii H. Magn.), Kudratovia candidogrisea (for Rinodina candidogrisea Hafellner, Muggia et Obermayer), Kudratovia luridata (for Buellia luridata Körb.), Kudratovia metaboliza (for Rinodina metaboliza Vain.), Kudratovia pycnocarpa (for Rinodina pycnocarpa H. Magn.), Kudratovia roscida (for Lecanora roscida Sommerf.), Kudratovia straussii (for Rinodina straussii J. Steiner), Kudratovia terrestris (for Rinodina terrestris Tomin), Kurokawia bryorum (for Anaptychia bryorum Poelt), Kurokawia isidiata (for Anaptychia isidiata Tomin), Kurokawia mereschkowskii (for Physcia mereschkowskii Tomin), Kurokawia palmulata (for Psoroma palmulatum Michx.), Kurokawia runcinata (for Lichen runcinatus With.), Kurokawia stippea (for Parmelia aquila var. stippea Ach.), Lecania safavidiorum (for Oxnerella safavidiorum S. Y. Kondr., Zarei-Darki, Lőkös et Hur), Leucodermia erinacea (for Lichen erinaceus Ach.), Mischoblastia confragosula (for Lecanora confragosula Nyl.), Mischoblastia destituta (for Lecidea destituta Nyl.), Mischoblastia moziana (for Lecanora moziana Nyl.), Mischoblastia moziana subsp. parasitica (comb. et stat. nova for Rinodina moziana var. parasitica Kaschik et H. Mayrhofer), Mischoblastia ramboldii (for Rinodina ramboldii Kaschik), Mischoblastia vezdae (for Rinodina vezdae H. Mayrhofer), Oxnerella afghanica (for Rinodina afghanica M. Steiner et Poelt), Oxnerella castanomelodes (for Rinodina castanomelodes H. Mayrhofer et Poelt), Physciella nigricans (for Lecanora nigricans Flörke), Poeltonia elegantula (for Physconia elegantula Essl.), Poeltonia grisea (for Lichen griseus Lam.), Poeltonia isidiomuscigena (for Physconia isidiomuscigena Essl.), Poeltonia perisidiosa (for Physcia perisidiosa Erichsen), Poeltonia venusta (for Parmelia venusta Ach.), and Polyblastidium albicans (for Parmelia albicans Pers.) are proposed.

Purpose. Economic security is an important component of any system, whether it is a state or a society, or a family. It reflects the ability to maintain and create conditions for restoring economic well-being and protecting interests. According to the political situation in Ukraine, the protection of economic security has become one of the most important issues for resolution. In recent years, the agrarian sector has become one of the key parts for GDP since the industrial business has now turned to the background. The purpose of the article is to determine the economic security level in the agrarian sector. Methodology. The authors have used the questionnaire method and have made a survey in 67 Ukrainian companies which are operating in the agriculture in the Lviv region, Ukraine. The survey was conducted from June to July 2018. Findings. Survey results show that enterprises have an extremely negative attitude to the state of economic security of the country. However, it should be noted that entrepreneurs believe that the level of economic security of their business is at a higher level in comparing with the level of economic security of the country. Research limitations/implications. As noted above, the study was conducted at 67 agribusiness enterprises in the Lviv region. In the future, this study may be the basis for the same research in other regions of Ukraine. Practical implications. In the questionnaire template, we have made the list of methods which can help to prevent the effects of negative factors that could damage the company's economic security. The list of these measures can be used by enterprises in practice to ensure a high level of its security. Originality/Value. It should be noted that the originality of our work is that we have combined the research of two areas: the economic security of the company and agribusiness. We have identified the main problems of agrarian sector in ensuring the economic security of enterprises and provided a list of methods to improve the level of this type of security, the use of which will lead to a better functioning of the firm. Keywords: economic security, agrarian sector, GDP, Ukraine; Research type (choose one): research paper. JEL classification: О11, О13, Q12.

Struktur komunitas dapat digunakan untuk mengetahui ukuran jumlah dari kumpulan berbagai populasi yang hidup dalam suatu waktu dan daerah tertentu yang saling berinteraksi dan mempengaruhi satu sama lain. Penelitian ini bertujuan untuk mengetahui, struktur komunitas, dan parameter fisika-kimia lingkungan bagi kehidupan Siput Laut di Dusun Karang Utara, Pulau Lemukutan. Penelitian ini menggunakan teknik purposive sampling untuk mempermudah penentuan stasiun pengamatan. Metode penentuan plot sampel yang digunakan adalah metode stratified random sampling. Plot sampel dibentangkan di sepanjang zona pasang surut (intertidal zone). Siput Laut yang ditemukan di lokasi penelitian ini berjumlah 26 spesies yang terdiri dari 14 famili. Jenis Siput Laut terbanyak adalah anggota Famili Cherithiidae dengan jumlah 9 spesies. Berdasarkan hasil perhitungan didapat 4 spesies tertinggi yaitu Clypeomorus moniliferus, Cronia margariticola, Rhinoclavis articula dan Rhinoclavis bituberculata. Nilai indeks dari hasil perhitungan didapat antara lain : indeks keanekaragaman (H’) dengan nilai antara 0,66-2,02, Nilai indeks kemerataan (E) antara 0,24-0,83, nilai indeks dominansi (C) antara 0,16-0,70. Pengukuran parameter lingkungan yang dipeoleh dengan suhu air yang berkisar rata-rata antara 29-30,66°C, sedangkan parameter kimia yang diukur meliputi salinitas dengan kisaran nilai rata-rata antara 29,66-30‰, nilai ph berkisar rata-rata antara7,64-7,88, dan DO dengan kisaran nilai rata-rata antara 4,1-7,53 mg/l nilai masing-masing parameter fisika-kimia lingkungan ini masih tergolong layak bagi kehidupan Siput Laut.

In Zambia, wild edible terrestrial orchids are used to produce a local delicacy called chikanda, which has become increasingly popular throughout the country. Commercialization puts orchid populations in Zambia and neighbouring countries at risk of overharvesting. Hitherto, no study has documented which orchid species are traded on local markets, as orchid tubers are difficult to identify morphologically. In this study, the core land-plant DNA barcoding markers rbcL and matK were used in combination with nrITS to determine which species were sold in Zambian markets. Eighty-two interviews were conducted to determine harvesting areas, as well as possible sustainability concerns. By using nrITS DNA barcoding, a total of 16 orchid species in six different genera could be identified. Both rbcL and matK proved suitable to identify the tubers up to the genus or family level. Disa robusta, Platycoryne crocea and Satyrium buchananii were identified most frequently and three previously undocumented species were encountered on the market. Few orchid species are currently listed on the global International Union for the Conservation of Nature (IUCN) Red List. Local orchid populations and endemic species could be at risk of overharvesting due to the intensive and indiscriminate harvesting of chikanda orchids, and we therefore encourage increased conservation assessment of terrestrial African orchids.

The present study investigated the growth performance, feed utilization, intestinal morphology, and microbiota communities of juvenile large yellow croaker (Larimichthys crocea) fed diets containing different proportions of soy protein concentrate (SPC) (0, 15%, 30%, and 45%, namely FM, SPC15, SPC30, and SPC45) as a substitute for fish meal (FM) for 8 weeks. The weight gain (WG) and specific growth rate (SGR) in fish fed SPC45 were significantly lower than those fed FM and SPC15 but not differ with these fed SPC30. The feed efficiency (FE) and protein efficiency ratio (PER) decreased sharply when the dietary SPC inclusion level was higher than 15%. The activity of alanine aminotransferase (ALT) and expression of alt and aspartate aminotransferase (ast) were significantly higher in fish fed SPC45 than those fed FM. The activity and mRNA expression of acid phosphatase were opposite. The villi height (VH) in distal intestine (DI) showed a significant quadratic response to increasing dietary SPC inclusion levels and was highest in SPC15. The VH in proximal intestine, middle intestine decreased significantly with increasing dietary SPC levels. The 16S rRNA sequences in intestine revealed that fish fed SPC15 had higher bacterial diversity and abundance of Phylum Firmicutes such as order Lactobacillales and order Rhizobiaceae than those fed other diets. Genus vibrio, family Vibrionaceae and order Vibrionales within phylum Proteobacteria were enriched in fish fed FM and SPC30 diets. Tyzzerella and Shewanella that belongs to phylum Firmicutes and Proteobacteria, respectively, were enriched in fish fed SPC45 diet. Our results indicated that SPC replacing more than 30% FM could lead to lower quality diet, retard growth performance, ill health, disordered intestine structure, and microbiota communities. Tyzzerella could be the bacteria indicator of intestinal in large yellow croaker fed low quality diet due to high SPC content. Based on the quadratic regression analysis of WG, the best growth performance could be observed when the replacement of FM with SPC was 9.75%.

Abstract Background: Factor V Leiden (FVL) mutation and Protein gene G20210A mutation (PGM) are the most common inherited thrombophilias in the world. (Limdi NA et.al, Blood Cells Mol Dis. 2006 Sep-Oct;37(2):100-6) Both are inherited in an autosomal recessive fashion with individuals who are homozygous having higher risk of thrombosis compared to those who are heterozygous.(Rodger MA et.al, PLoS Med. 2010 Jun 15;7(6):e1000292.) The global prevalence of FVL and PGM is variable with Caucasians carrying the highest prevalence and Africans living in Africa, Asians and Native Americans having the lowest rate of these mutations; it is zero in West and Southern African countries, 2.4%-3.9% in North African countries including Morocco, Tunisia, and Algeria. (Limdi NA et.al, Blood Cells Mol Dis. 2006 Sep-Oct;37(2):100-6, Dziadosz M et. al, Blood Coagul Fibrinolysis. 2016 Jul;27(5):481-9) Pregnancy increases the risk of developing venous thromboembolism (VTE) by 0.05-1.8 %, (Eldor A, Thromb Haemost. 2001 12.12.2017;86(07):104-11) which is 4 to 5 fold greater than in non-pregnant female.(Croles FN et.al, BMJ. 2017;359, Greer IA, N Engl J Med. 2015 Aug 6;373(6):540-7) Inheritance of FVL and PGM increases the risk for development of VTE in pregnancy, the risk is higher with homozygous than heterozygous mutations.(Rodger MA et.al, PLoS Med. 2010 Jun 15;7(6):e1000292.) Concurrent presentation of FVL and PGM in pregnancy presenting as thrombosis is not common. Aim: The aim of this case report is to present a patient of African descent with concurrent FVL and PGM mutation who had thrombosis during pregnancy. The Case: A 32-year-old Nigerian female who was 28 weeks pregnant presented to the gynecologist with a swollen left leg. Physical examination and the Wells score were very suggestive of a deep vein thrombosis (DVT). The Duplex Doppler performed confirmed a diagnosis of bilateral lower limb DVT. The patient was referred to a physician, who together with the haematologist performed a thrombophilia screen including FVL, PGM, Protein C, Protein S and Anticardiolipin antibody tests. The D-DIMERS were raised and the viral markers including HIV, HBV, and HCV were negative. The PT, APTT and full blood counts were normal. The thrombophilia tests revealed that the patient was homozygous for FVL and also heterozygous for the PGM mutations. The rest of the thrombophilia screen including Protein C, Protein S and Antithrombin tests were all negative. She has no family history of thrombosis; no past history of hormone based contraceptives. She was counselled on the need for using anticoagulation, low molecular weight heparin (LMWH) during the rest of her pregnancy period, and therapeutic anticoagulation with a LMWH at a dose of 1mg/kg twice a day until onset of labour was started. During labour LMWH was discontinued, delivery was per vaginal and was uncomplicated. Postpartum LMWH was continued at therapeutic doses for six weeks with no bleeding or thrombotic events. After six weeks, the patient was started on lifelong warfarin. Results (See Table) Discussion: To our knowledge this is a first case of a patient born and living in Africa presenting with thrombosis and found to have homozygous FVL and heterozygous PGM during pregnancy, the one report that is similar was in a second generation South African woman of German, Dutch and French ancestry. (Wilson J et. al, Medical Technology SA. [Case Report]. December 2011;25(2):4) Most reports have shown ethnic and regional affectation. (Limdi NA et.al, Blood Cells Mol Dis. 2006 Sep-Oct;37(2):100-6, Bavikatty NR, et.al, Am J Clin Pathol. 2000 Aug;114(2):272-5) There is no single agreed reason as to why the mutation is rare in Africa though founder effect is being studied to define how the mutation came about, it could have taken place before divergence of race. (Jadaon MM. [Thrombosis, Familiar Thrombophilia]. 2011 2011-11-28;3(1) We could not do family studies to help define the origin of this mutation whether it is denovo for Nigeria; this has to do with sentiments about genetic inheritance in Africa. Conclusion: Concurrent inheritance of both FVL and PGM is possible in Africa. This rare case has open opportunities to revisit the prevalence of thrombophilia in Nigeria and other African countries. Disclosures Mahlangu: Alnylam: Consultancy, Research Funding, Speakers Bureau; Biomarin: Research Funding, Speakers Bureau; Catalyst Biosciences: Consultancy, Research Funding; Chugai: Consultancy; Amgen: Consultancy; Bayer: Research Funding; Biogen: Research Funding, Speakers Bureau; CSL Behring: Consultancy, Research Funding, Speakers Bureau; NovoNordisk: Consultancy, Research Funding, Speakers Bureau; LFB: Consultancy; Roche: Consultancy, Research Funding, Speakers Bureau; Sanofi: Research Funding, Speakers Bureau; Shire: Consultancy, Research Funding, Speakers Bureau; Sobi: Research Funding, Speakers Bureau; Spark: Consultancy, Research Funding.

ABSTRACT Popular and scholarly information concerning the life of James Croll has been accumulating slowly since the death in 1890 of the self-taught climate change pioneer. The papers in the current volume offer thorough assessments of topics associated with Croll's work, but this contribution seeks to provide a personal context for an understanding of James Croll the man, as well as James Croll the scholar of sciences and religion. Using archival as well as published sources, emphasis is placed upon selected components of his life and some of the less recognised features of his biography. These include his family history, his many homes, his health, participation in learned societies and attitudes to collegiality, financial problems including the failed efforts to secure a larger pension, and friendship. Life delivered a mixture of ‘trials and sorrows’, but it seems clear from the affection and respect accorded him that many looked upon James Croll as a ‘man greater far than his work’.

ABSTRACT The quality and richness of Perthshire's natural environment were formative influences on a young James Croll (1821–1890), which left him with a life-long appreciation of nature, landscape and natural meditation. Although Croll himself declares to have had little interest in geology in his earlier years, it became a central theme of his scientific understanding, which implies the clear influence of both his local environment and of his father David, a stonemason. His family and friends also shaped him in other ways, not least his love of reading, his unconstrained thinking and intellectual acuity. He inherited his father's moral character, amiability and an excitement about intellectual inquiry, which drew friends to him who made great efforts to assist him in his work, both personally and professionally, and played a role in his being offered a position by James Geikie with the Geological Survey of Scotland. Croll's financial position was often precarious; he spent a good deal of his life in relative poverty. Whilst this affected his opportunities for formal learning, it may well have led to his ability to think creatively and to seek answers more broadly than he might have if he had been able to engage in a more formal education. Ill health, which affected him throughout his life, could be seen to both hamper his work – but also through circumstance lead him to pursue a more academic path, as other routes of work were shut off to him. Ultimately Whitefield, Wolfhill and the wider Perthshire countryside in which he grew up can clearly be seen to have influenced his life in many ways, even, perhaps, to the extent of his chosen surname.

Doublesex- and mab-3-related transcription factor B1 (dmrtb1/dmrt6) belongs to one of the members of DMRT family, which performs critical functions in sex determination and differentiation, gonadal development, and functional maintenance. However, knowledge of its exact mechanism remains unclear in teleost. Very little is known about the role of dmrtb1 in the gonad development of Larimichthys crocea. In this study, a dmrtb1 homolog in L. crocea named as Lcdmrtb1 with the full-length cDNA was isolated and characterized. Except for the conserved DM domain, the other regions had low homology. Of the tissues sampled, Lcdmrtb1 was only found to be highly expressed in testis. In situ hybridization of testis revealed Lcdmrtb1 in both spermatogonia and spermatocytes. After Lcdmrtb1 interference in the testis cells (LYCT) of L. crocea, the expression levels of Lcdmrtb1 and Lcdmrt1 were significantly decreased; subsequently, testicular cell morphology changed from fibrous to round and their growth rate slowed. Similarly, the expression levels of Lcdmrtb1, Lcdmrt1, sox9a/b and amh were significantly decreased after RNAi in testis. Furthermore, it was discovered that the spermatogonia had disappeared, and the Sertoli cells had been reduced. The results of immunohistochemistry showed that the expression of Sox9 protein in testis was not detected after dmrtb1 was knocked down. These results indicated that the absence of Lcdmrtb1 not only greatly inhibited cell growth and destroyed morphology of testis cells but also down-regulated Lcdmrt1 expression in testis. This study will be helpful in understanding further the molecular regulation mechanism of Lcdmrtb1/Lcdmrt6 in testicular development in L. crocea.

P-element-induced wimpy testis (Piwi) is a key gene involved in germ cell development in a diverse range of organisms. However, in giant clams, the function of Piwi remains unclear. In the present study, we isolated the full-length cDNA of Piwi ortholog (Tc-Piwi1) and analyzed its expression patterns in the gonads of adult and juvenile Tridacna crocea. The results of qPCR showed that the transcript of Tc-Piwi1 was mainly expressed in gonad tissue. In addition, the relative expression level of Tc-Piwi1 increased with the proliferation of male and female germ cells during the adult gonad development stage, suggesting that Tc-Piwi1 might be involved in gametogenesis. In situ hybridization revealed that Tc-Piwi1 RNA was located in female and male germ cells and strongly expressed in male germ cells in the early stage. Furthermore, immunohistochemical experiments further confirmed that Tc-Piwi1 was mainly located in primordial germ cells (PGCs), germ stem cells (GSCs), and female and male germ cells of early development, so it could be used as a marker gene of T. crocea germ cells. Whole-mount in situ hybridization suggested that Tc-Piwi1 was of maternal origin and located in two clusters of cells in the trochophore-larvae stage, implying that these cells might be putative PGCs during the embryo development. Finally, Tc-Piwi1 was used as a molecular marker to elucidate the gonadal formation, sex differentiation, and gonadal maturation process of juvenile T. crocea for the first time in the Tridacna family. Collectively, all these results revealed that Tc-Piwi1 was involved in germline formation and sex differentiation in T. crocea.

Abstract Teleost fish type I IFNs and the associated receptors from the cytokine receptor family B (CRFB) are characterized by remarkable diversity and complexity. How the fish type I IFNs bind to their receptors is still not fully understood. In this study, we demonstrate that CRFB1 and CRFB5 constitute the receptor pair through which type I subgroup d IFN (IFNd) from large yellow croaker, Larimichthys crocea, activates the conserved JAK-STAT signaling pathway as a part of the antiviral response. Our data suggest that L. crocea IFNd (LcIFNd) has a higher binding affinity with L. crocea CRFB5 (LcCRFB5) than with LcCRFB1. Furthermore, we report the crystal structure of LcIFNd at a 1.49-Å resolution and construct structural models of LcIFNd in binary complexes with predicted structures of extracellular regions of LcCRFB1 and LcCRFB5, respectively. Despite striking similarities in overall architectures of LcIFNd and its ortholog human IFN-ω, the receptor binding patterns between LcIFNd and its receptors show that teleost and mammalian type I IFNs may have differentially selected helices that bind to their homologous receptors. Correspondingly, key residues mediating binding of LcIFNd to LcCRFB1 and LcCRFB5 are largely distinct from the receptor-interacting residues in other fish and mammalian type I IFNs. Our findings reveal a ligand/receptor complex binding mechanism of IFNd in teleost fish, thus providing new insights into the function and evolution of type I IFNs.

Large yellow croaker Larimichthys crocea (Richardson) is an important member in family Sciaenidae, and one of most productive mariculture fishes in China. Fluorescence in situ hybridization is a useful tool for cytogenetics and genomics research. Here, we demonstrated that bacterial artificial chromosome (BAC) clones could be used to identify individual chromosomes in large yellow croaker, and also to study chromosome evolution in the related species. By using BAC paired-end sequencing and sequence alignment, 435 BAC clones were anchored to 24 pseudochromosomes of large yellow croaker genome. Among them, 72 BAC clones with low repeat content were selected and passed PCR test, and then grouped by chromosome for FISH test. As a results, there were 67 BAC clones, 2 to 3 BAC clones per chromosome, generated specific and stable signal at expected position. Then, a dual-color FISH probe cocktail composed of 48 of these BAC clones was formulated and used to hybridize metaphase chromosome spreads, resulting in distinct signal patterns on each chromosome pair, which help to distinguish all chromosomes in the metaphase spreads of large yellow croaker. The chromosome-specific BAC-FISH probes were also applied to a close relative of large yellow croaker, Collichthys lucidus, demonstrating that its Y chromosome originated from the fusion of Chr.1 and Chr.7. Thus, our study provides the first set of chromosome-specific FISH probes in family Sciaenidae, which will play an important role in cytogenetics and genomics research in the family.

In the neuroendocrine system, corticotropin-releasing hormone (CRH) plays an important role in the hypothalamic–pituitary–adrenal/interrenal (HPA/HPI) axis. It exerts its effects by activating CRHRs, which belong to the class B G protein-coupled receptor family. Two characteristic genes of CRHR1 subtypes in the Larimichthys crocea genome were identified: LcCRHR1-1 and LcCRHR1-2. Alignments indicated that they were highly homologous to known and validated teleost CRHR1s. The CDS sequences of the two receptors were cloned into the pEGFP-N1 plasmid, and membrane localization of the fusion expressing LcCRHR1-1-EGFP and LcCRHR1-2-EGFP was revealed in HEK293 cells. Treatment with LcCRH could lead to two receptors internalization and trigger a significant increase in the secondary messenger cAMP and Ca2+ and mitogen-activated protein kinase phosphorylation in an LcCRH dose-dependent manner. Based on quantitative real-time PCR, LcCRHR1s were expressed in all examined tissues and highly expressed in the brain and ovaries. Furthermore, immunohistochemical findings showed the specific localization of CRHR1s in ovarian follicle cells. Collectively, our study identified two CRH receptors in L. crocea and suggested that the CRH/CRHR1 system is potentially involved in the neuroendocrine regulation of reproduction in this marine fish.

Consensus and sex-specific genetic linkage maps for large yellow croaker (Larimichthys crocea) were constructed using samples from an F1 family produced by crossing a Daiqu female and a Mindong male. A total of 20,147 single nucleotide polymorphisms (SNPs) by restriction site associated DNA sequencing were assigned to 24 linkage groups (LGs). The total length of the consensus map was 1757.4 centimorgan (cM) with an average marker interval of 0.09 cM. The total length of female and male linkage map was 1533.1 cM and 1279.2 cM, respectively. The average female-to-male map length ratio was 1.2 ± 0.23. Collapsed markers in the genetic maps were re-ordered according to their relative positions in the ASM435267v1 genome assembly to produce integrated genetic linkage maps with 9885 SNPs distributed across the 24 LGs. The recombination pattern of most LGs showed sigmoidal patterns of recombination, with higher recombination in the middle and suppressed recombination at both ends, which corresponds with the presence of sub-telocentric and acrocentric chromosomes in the species. The average recombination rate in the integrated female and male maps was respectively 3.55 cM/Mb and 3.05 cM/Mb. In most LGs, higher recombination rates were found in the integrated female map, compared to the male map, except in LG12, LG16, LG21, LG22, and LG24. Recombination rate profiles within each LG differed between the male and the female, with distinct regions indicating potential recombination hotspots. Separate quantitative trait loci (QTL) and association analyses for growth related traits in 6 months fish were performed, however, no significant QTL was detected. The study indicates that there may be genetic differences between the two strains, which may have implications for the application of DNA-information in the further breeding schemes.

Abstract Lutjanus erythropterus (Bloch, 1790), a Perciforme from the Lutjanidae family, is a commercially important species due to its taste and abundance. Despite the increase in genome resources in recent years, few genome assemblies are available within this fish family for comparative and functional studies. In this study, we determined the chromosomal genome of Crimson snapper using high-throughput stLFR sequencing technology and Hi-C data. The final assembly size was 973.04 Mb with contig and scaffold N50 values of 1.51 Mb and 40.65 Mb respectively. We successfully scaffolded 95.84% of the genome sequence onto 24 chromosomes ranging in length from 19.37 Mb to 49.48 Mb. A total of 22,663 genes and 13,877 gene families were identified in the genome with 29 gene families being L. erythropterus specific. Phylogenetic analysis using single-copy gene families showed that L. erythropterus and Larimichthys crocea had the closest genetic relationship with a divergence time of approximately 47.7 Mya. This new genomic resource will facilitate comparative genomics studies as well as genetic breeding programs for L.erythropterus.

Spiny head croaker (Collichthys lucidus), belonging to the family Sciaenidae, is a small economic fish with a main distribution in the coastal waters of Northwestern Pacific. Here, we constructed a nonredundant chromosome-level genome assembly of spiny head croaker and also made genome-wide investigations on genome evolution and gene families related to otolith development. A primary genome assembly of 811.23 Mb, with a contig N50 of 74.92 kb, was generated by a combination of 49.12-Gb Illumina clean reads and 35.24 Gb of PacBio long reads. Contigs of this draft assembly were further anchored into chromosomes by integration with additional 185.33-Gb Hi-C data, resulting in a high-quality chromosome-level genome assembly of 817.24 Mb, with an improved scaffold N50 of 26.58 Mb. Based on our phylogenetic analysis, we observed that C. lucidus is much closer to Larimichthys crocea than Miichthys miiuy. We also predicted that many gene families were significantly expanded (p-value <0.05) in spiny head croaker; among them, some are associated with “calcium signaling pathway” and potential “inner ear functions.” In addition, we identified some otolith-related genes (such as otol1a that encodes Otolin-1a) with critical deletions or mutations, suggesting possible molecular mechanisms for well-developed otoliths in the family Sciaenidae.

Abstract Angiopoietin-like 4 (ANGPTL4) is a potent regulator of triacylglycerol metabolism but knowledge of the mechanisms underlying ANGPTL4 transcription in response to fatty acids is still limited in teleost. In this study, we explored the molecular characterization of ANGPTL4 and regulatory mechanisms of ANGPTL4 in response to fatty acids in large yellow croaker (Larimichthys crocea). Here, croaker angptl4 contained a 1416 bp open reading frame encoding a protein of 471 amino acids with highly conserved 12-amino acid consensus motif. Angptl4 was widely expressed in croaker, with the highest expression in the liver. In vitro, oleic and palmitic acids (OA and PA) treatments strongly increased angptl4 mRNA expression in croaker hepatocytes. Moreover, angptl4 expression was positively regulated by peroxisome proliferator-activated receptor family (PPAR-α, β and γ) and expression of pparγ was also significantly increased in response to OA and PA. Moreover, inhibition of PPARγ abrogated OA or PA-induced angptl4 mRNA expression. Beyond that, PA might increase angptl4 expression partly via the insulin signaling. Overall, the expression of ANGPTL4 is strongly upregulated by OA and PA via PPARγ in the liver of croaker, which contributes to improve the understanding of the regulatory mechanisms of ANGPTL4 in fish.

Zinc finger Asp-His-His-Cys motif-containing (zDHHC) proteins, known for their palmitoyltransferase (PAT) activity, play crucial roles in diverse cellular processes, including immune regulation. However, their non-palmitoyltransferase immunomodulatory functions and involvement in teleost immune responses remain underexplored. In this study, we systematically characterized the zDHHC family in the large yellow croaker (Larimichthys crocea), identifying 22 members. Phylogenetic analysis unveiled that each of the 22 LczDHHCs formed distinct clusters with their orthologues from other teleost species. Furthermore, all LczDHHCs exhibited a highly conserved DHHC domain, as confirmed by tertiary structure prediction. Notably, LczDHHC23 exhibited the most pronounced upregulation following Pseudomonas plecoglossicida (P. plecoglossicida) infection of macrophage/monocyte cells (MO/MΦ). Silencing LczDHHC23 led to heightened pro-inflammatory cytokine expression and diminished anti-inflammatory cytokine levels in MO/MΦ during infection, indicating its anti-inflammatory role. Functionally, LczDHHC23 facilitated M2-type macrophage polarization, as evidenced by a significant skewing of MO/MΦ towards the pro-inflammatory M1 phenotype upon LczDHHC23 knockdown, along with the inhibition of MO/MΦ necroptosis induced by P. plecoglossicida infection. These findings highlight the non-PAT immunomodulatory function of LczDHHC23 in teleost immune regulation, broadening our understanding of zDHHC proteins in host-pathogen interactions, suggesting LczDHHC23 as a potential therapeutic target for immune modulation in aquatic species.

The study examined the key gene Dishevelled (Dvl or Dsh) in Wnt (Wingless and INT-1) signaling pathways. The gene (Dvl) was characterized in the flat fish Paralichthys olivaceus for its expression pattern structure and phylogenetics at the Ocean University of China in Qingdao, China in 2018. Three gene paralogues (Dvl1, Dvl2 and Dvl3) of the Dvl family were cloned in P. olivaceus and a N-terminal DAX domain, a central PDZ domain and a C-terminal DEP domain were discovered in all three protein paralogues. Phylogenetic analysis revealed that Dvl genes in P. olivaceus are most closely related to those in marine teleosts Larimichthys crocea and Stegastes partitus, followed by those in Cynoglossus semilaevis. For each Dvl gene, the genes in teleosts fall into a clade independent from the ones in other vertebrates, suggesting that the duplication of Dvl genes occurred prior to the divergence of vertebrates. The temporal expression patterns of the three Dvl genes were characterized during the embryonic development of teleosts. In P. olivaceus, all three Dvl genes remain at low expression levels during the early stages of development until gastrula stage, when the expression of Dvl1 was significantly up-regulated. The research revealed vastly different temporal expression patterns of Dvl genes and suggested that the structure of Dvl proteins is conserved, but the expression patterns of Dvl genes vary significantly among different classes.

Abstract Noctuidae are one of the world’s most diverse, ecologically successful, and economically important animal lineages with over 12,000 species in ~1,150 genera. We inferred a phylogeny using eight protein-coding genes for the global fauna, greatly expanding upon previous attempts to stabilize Noctuidae higher classification by sampling 341 genera (nearly half represented by their type species) representing 70/76 widely recognized family-group taxa: 20/21 subfamilies, 32/35 tribes, and 18/20 subtribes. We evaluated 17 subfamily-level taxa in detail, discussing adult and larval morphology, life histories, and taxonomic implications of our results. We significantly alter concepts of Acontiinae, Condicinae, Eustrotiinae, Metoponiinae, and Stiriinae. Our results supported recognition of two new subfamilies: Cobubathinae Wagner & Keegan, 2021 subf. nov. and Cropiinae Keegan & Wagner, 2021 subf. nov. Other nomenclatural changes we made are as follows. We moved: ‘Acontia’ viridifera (Hampson, 1910), ‘Azenia’ virida Barnes and McDunnough, 1916, Aleptinoides, Austrazenia, Chalcoecia, Megalodes, and Trogotorna to Chamaecleini in Acontiinae; Apaustis to, and reinstated Emmelia as a valid genus in Acontiinae; Allophyes and Meganephria to Cuculliinae; ‘Plagiomimicus’ navia (Harvey, 1875), Airamia, Alvaradoia, Hypoperigea, Neotarache, and Mesotrosta to Condicinae; Axenus, Azenia, Metaponpneumata, Sexserrata, and Tristyla to Metoponiinae; ‘Paramiana’ canoa (Barnes, 1907) to Noctuinae; Aucha, Cobubatha, and Tripudia to Cobubathinae; Anycteola and Supralathosea to Oncocnemidinae; Cropia to Cropiinae; Desmoloma to Dyopsinae; Eviridemas and Gloanna to Bryophilinae; Fota and Stilbia to Stiriinae; and Copibryophila, Homolagoa, and Tyta to Noctuidae incertae sedis. We conclude with discussion of instances where current understanding of noctuid biogeography and life histories were changed by our results.

Tumor necrosis factor receptor-associated factors (TRAFs) play vital roles in tumor necrosis factor receptor (TNF-R) and interleukin-1 receptor/Toll-like receptor (IL-1R/TLR) mediated signaling pathway. However, the role that TRAF7 plays in the host immune responses is largely unknown in comparison to the extensive and in-depth research that has been conducted on other members of the TRAF family. Notably, Lc-TRAF7, a cloned TRAF7 ortholog, was discovered in the large yellow croaker (Larimichthys crocea) in the current study, which has an open reading frame (ORF) of 1,962 base pairs and encodes a protein of 653 amino acids (aa). Lc-TRAF7 is consisted of a RING finger domain, a coiled-coil domain, and seven WD40 domains, with the genomic organization consisted of 20 exons and 19 introns. According to the expression analysis, Lc-TRAF7 was presented in a wide range of detected organs and tissues of the healthy fish, and was able to significantly induced by stimulations of poly I:C, LPS, PGN, and Pseudomonas plecoglossicida infection. Subcellular distribution analysis revealed that Lc-TRAF7 was a cytoplasmic protein, with the RING finger and coiled-coil domain function importantly in its subcellular localization. Luciferase assays demonstrated that Lc-TRAF7 overexpression significantly induced the activation of NF-κB, IRF3, IRF7, and IFN1 promoters. In addition, the WD40 domains play a pivotal role in the NF-κB promoter activation, whereas the RING finger and coiled-coil domain were essential in the IRF3, IRF7, and IFN1 promoter activation. Notably, Lc-TRAF7 overexpression could suppress SVCV proliferation in EPC cells, and the expression levels of IRF3, IRF7, ISG15, ISG56, RSAD2, and TNF-α were up-regulated under Lc-TRAF7 overexpression in LYCMS cells. These findings collectively implied that Lc-TRAF7 may function as an important regulator in the host antiviral responses via the NF-κB as well as IRF3/7 involved signaling pathways.

Each year, The Economist magazine’s “Economist Intelligence Unit” ranks cities based on “healthcare, education, stability, culture, environment and infrastructure”, giving the highest-ranking locale the title of most ‘liveable’ (Wright). For the past six years, The Economist has named Melbourne “the world’s most liveable city” (Carmody et al.). A curious portmanteau, the concept of liveability is problematic: what may feel stable and safe to some members of the community may marginalise others due to several factors such as gender, disability, ethnicity or class.The subjective nature of this term is referred to in the Australian Government’s 2013 State of Cities report, in the chapter titled ‘Liveability’:In the same way that the Cronulla riots are the poster story for cultural conflict, the attack on Jillian Meagher in Melbourne’s Brunswick has resonated strongly with Australians in many capital cities. It seemed to be emblematic of their concern about violent crime. Some women in our research reported responding to this fear by arming themselves. (274)Twenty-nine-year-old Jill Meagher’s abduction, rape, and murder in the inner northern suburb of Brunswick in 2012 disturbs the perception of Melbourne’s liveability. As news of the crime disseminated, it revived dormant cultural narratives that reinforce a gendered public/private binary, suggesting women are more vulnerable to attack than men in public spaces and consequently hindering their mobility. I investigate here how texts written by women writers based in Melbourne’s inner north can latently serve as counter narratives to this discourse, demonstrating how urban public space can be benign, even joyful, rather than foreboding for women. Cultural narratives that promote the vulnerability of women oppress urban freedoms; this paper will use these narratives solely as a catalyst to explore literary texts by women that enact contrary narratives that map a city not by vicarious trauma, but instead by the rich complexity of women’s lives in their twenties and thirties.I examine two memoirs set primarily in Melbourne’s inner north: Michele Lee’s Banana Girl (2013) and Lorelai Vashti’s Dress, Memory: A memoir of my twenties in dresses (2014). In these texts, the inner north serves as ‘true north’, a magnetic destination for this stage of life, an opening into an experiential, exciting adult world, rather than a place haunted. Indeed, while Lee and Vashti occupy the same geographical space that Meagher did, these texts do not speak to the crime.The connection is made by me, as I am interested in the affective shift that follows a signal crime such as the Meagher case, and how we can employ literary texts to gauge a psychic landscape, refuting the discourse of fear that is circulated by the media following the event. I wish to look at Melbourne’s inner north as a female literary milieu, a site of boldness despite the public breaking that was Meagher’s murder: a site of female self-determination rather than community trauma.I borrow the terms “boldness”, “bold walk” and “breaking” from Finnish geographer Hille Koskela (and note the thematic resonances in scholarship from a city as far north as Helsinki). Her paper “Bold Walks and Breakings: Women’s spatial confidence versus fear of violence” challenges the idea that “fearfulness is an essentially female quality”, rather advocating for “boldness”, seeking to “emphasise the emancipatory content of … [women’s] stories” (302). Koskela uses the term “breaking” in her research (primarily focussed on experiences of Helsinki women) to describe “situations … that had transformed … attitudes towards their environment”, referring to the “spatial consequences” that were the result of violent crimes, or threats thereof. While Melbourne women obviously did not experience the Meagher case personally, it nevertheless resulted in what Koskela has dubbed elsewhere as “increased feelings of vulnerability” (“Gendered Exclusions” 111).After the Meagher case, media reportage suggested that Melbourne had been irreversibly changed, made vulnerable, and a site of trauma. As a signal crime, the attack and murder was vicariously experienced and mediated. Like many crimes committed against women in public space, Meagher’s death was transformed into a cautionary tale, and this storying was more pronounced due to the way the case played out episodically in the media, particularly online, allowing the public to follow the case as it unfolded. The coverage was visually hyperintensive, and particular attention was paid to Sydney Road, where Meagher had last been seen and where she had met her assailant, Adrian Bayley, who was subsequently convicted of her murder.Articles from media outlets were frequently accompanied by cartographic images that superimposed details of the case onto images of the local area—the mind map and the physical locality both marred by the crime. Yet Koskela writes, “the map of everyday experiences is in sharp contrast to the maps of the media. If a picture of a place is made by one’s own experiences it is more likely to be perceived as a safe ordinary place” (“Bold Walks” 309). How might this picture—this map—be made through genre? I am interested in how memoir might facilitate space for narratives that contest those from the media. Here I prefer the word memoir rather than use the term life-writing due to the former’s etymological adherence to memory. In Vashti and Lee’s texts, memory is closely linked to place and space, and for each of them, Melbourne is a destination, a city that they have come to alone from elsewhere. Lee came to the city after growing up in Canberra, and Vashti from Brisbane. In Dress, Memory, Vashti writes that the move to Melbourne “… makes you feel like a pioneer, one of those dusty and determined characters out of an American history novel trudging west to seek a land of gold and dreams” (83).Deeply engaging with Melbourne, the text eschews the ‘taken for granted’ backdrop idea of the city that scholar Jane Darke observes in fiction. She writes thatmodern women novelists virtually take the city as backdrop for granted as a place where a central female figure can be or becomes self-determining, with like-minded female friends as indispensable support and undependable men in walk-on roles. (97)Instead, Vashti uses memoir to self-consciously examine her relationship with her city, elaborating on the notion of moving from elsewhere as an act of self-determination, building the self through geographical relocation:You’re told you can find treasure – the secret bars hidden down the alleyways, the tiny shops filled with precious curios, the art openings overflowing onto the street. But the true gold that paves Melbourne’s footpaths is the promise that you can be a writer, an artist, a musician, a performer there. People who move there want to be discovered, they want to make a mark. (84)The paths are important here, as Vashti embeds herself on the street, walking through the text, generating an affective cartography as her life is played out in what is depicted as a benign, yet vibrant, urban space. She writes of “walking, following the grid of the city, taking in its grey blocks” (100), engendering a sense of what geographer Yi-Fu Tuan calls ‘topophilia’: “the affective bond between people and place or setting” (4). There is a deep bond between Vashti and Melbourne that is evident in her work that is demonstrated in her discussion of public space. Like her, friends from Brisbane trickle down South, and she lives with them in a series of share houses in the inner North—first Fitzroy, then Carlton, then North Melbourne, where she lives with two female friends and together they “roamed the streets during the day in a pack” (129).Vashti’s boldness not only lies in her willingness to take bodily to the streets, without fear, but also in her fastidious attention to her physical appearance. Her memoir is framed sartorially: chronologically arranged, from age twenty to thirty, each chapter featuring equally detailed reports of the events of that year as well as the corresponding outfits worn. A dress, transformative, is spotlighted in each of these chapters, and the author is photographed in each of these ‘feature’ dresses in a glossy section in the middle of the book. Koskela writes that, “if women dress up to be part of the urban spectacle, like 19th-century flâneurs, and also to mediate their confidence, they oppose their erasure and reclaim urban space”. For Koskela, the appearance of the body in public is an act of boldness:dressing can be seen as a means of reproducing power relations; in Foucaultian terms, it is a way of being one’s own overseer, and regulating even the most intimate spheres … on the other hand, interpreted in another way, dressing up can be seen as a form of resistance against the male gaze, as an opposition to the visual mastery over women, achieved by not being invisible or absent, but by dressing up proudly. (“Bold Walks” 309)Koskela’s affirmation that clothing can enact urban boldness contradicts reportage on the Meagher case that suggested otherwise. Some news outlets focussed on the high heels Meagher was wearing the night she was raped and murdered, as if to imply that she may have been able to elude her fate had she donned flats. The Age quotes witnesses who saw her on Sydney Road the night she was killed; one says she was “a little unsteady on her feet but not too bad”, another that she “seemed to be struggling to walk up the hill in her high heels” (Russell). But Vashti is well aware of the spatial confidence that the right clothing provides. In the chapter “Twenty-three”, she writes of being housebound by heartbreak, that “just leaving the house seemed like an epic undertaking”, so she “picked a dress a dress that would make me feel good … the woman in me emerged when I slid it on. In it, I instantly had shape, form. A purpose” (99). She and her friends don vocational costumes to outplay the competitive inner Melbourne rental market, eventually netting their North Melbourne terrace house by dressing like “young professionals”: “dressed up in smart op-shop blouses and pencil skirts to walk to the real estate office” (129).Michele Lee’s text Banana Girl also delves into the relationship between personal aesthetics and urban space, describing Melbourne as “a town of costumes, after all” (117), but her own style as “indifferently hip to the outside world without being slavish about it” (6). Lee’s world is East Brunswick for much of the book, and she establishes this connection early, introducing herself in the first chapter, as one of the “subversive and ironic people living in the hipster boroughs of the inner North of Melbourne” (6). She describes the women in her local area – “Brunswick Girls”, she dubs them: “no one wears visible make up, or if they do it’s not lathered on in visible layers; the haircuts are feminine without being too stylish, the clothing too; there’s an overall practical appearance” (89).Lee displays more of a knowingness than Vashti regarding the inner North’s reputation as the more progressive and creative side of the Yarra, confirmed by the Sydney Morning Herald:The ‘northside’ comprises North Melbourne, Carlton, Fitzroy, Collingwood, Abbotsford, Thornbury, Brunswick and Coburg. Bell Street is the boundary for northsiders. It stands for artists, warehouse parties, bicycles, underground music, lightless terrace houses, postmodernity and ‘awareness’. (Craig)As evidenced in late scholar John Maclaren’s book Melbourne: City of Words, the area has long enjoyed this reputation: “After the war, these neighbourhoods were colonized by migrants from Europe, and in the 1960s by the artists, musicians, writers, actors, junkies and layabouts whose stories Helen Garner was to tell” (146). As a young playwright, Lee sees herself reflected in this milieu, writing that she’s “an imaginative person, I’m university educated, I vote the way you’d expect me to vote and I’m a member of the CPSU. On principle I remain a union member” (7), toeing that line of “awareness” pithily mentioned by the SMH.Like Vashti, there are constant references to Lee’s exact geographical location in Melbourne. She ‘drops pins’ throughout, cultivating a connection to place that blurs home and the street, fostering a sense of belonging beyond one’s birthplace, belonging to a place chosen rather than raised in. She plants herself in this local geography. Returning to the first chapter, she includes “jogger by the Merri Creek” in her introduction (7), and later jokingly likens a friendship with an ex as “no longer on stage at the Telstra Dome but still on tour” (15), employing Melbourne landmarks as explanatory shorthand. She refers to places by name: one could physically tour inner North and CBD hotspots based on Lee’s text, as it is littered with mentions of bars, restaurants, galleries and theatre venues. She frequents the Alderman in East Brunswick and Troika in the city, as well as a bar that Jill Meagher spent time in on the night she went missing – the Brunswick Green.While offering the text a topographical authenticity, this can sometimes prove distracting: rather than simply stating that she goes to the library, she writes that she visits “the City of Melbourne library” (128), and rather than just going to a pizza parlour, they visit “Bimbo’s” (129) or “Pizza Meine Liebe” (101). Yet when Lee visits family in Canberra, or Laos on an arts grant, business names are forsaken. One could argue that the cultural capital offered by namedropping trendy Melburnian bars, restaurants and nightclubs translates awkwardly on the page, and risks dating the text considerably, but elevates the spatiality of Lee’s work. And these landmarks are important within the text, as Lee’s world is divided spatially. She refers to “Theatre Land” when discussing her work in the arts, and her share house not as ‘home’ but consistently as “Albert Street”. She partitions her life into these zones: zones of emotion, zones of intellect/career, zones of family/heritage – the text offers close insight into Lee’s personal cartography, with her traversing the map “stubbornly on foot, still resisting becoming part of Melbourne’s bike culture” (88).While not always walking alone – often accompanied by an ex-boyfriend she nicknames “Husband” – Lee is independently-minded, stating, “I operate solo, I pay my own way” (34), meeting up with various romantic and sexual interests through the text for daytime trysts in empty office buildings or late nights out in the CBD. She is adventurous, yet reminds that she was not always so. She recalls a time when she was still residing in Canberra and visited a boyfriend who was living in Melbourne and felt intimidated by the “alien city”, standing in stark contrast to the familiarity she demonstrates otherwise.Lee and Vashti’s texts both chronicle women who freely occupy public space, comfortable in their surroundings, not engaging on the page with cultural narratives and media reportage that suggest they would be safer off the streets. Both demonstrate what Koskela calls the “pleasure to be able to take possession of space” (“Bold Walks” 308) – yet it could be argued that the writer’s possession of space is so routine, so unremarkable that it transcends pleasure: it is comfortable. They walk the streets alone and catch public transport alone without incident. They contravene advice such as that given by Victorian Police Homicide Squad chief Mick Hughes’s comments that women shouldn’t be “alone in parks” following the fatal stabbing of teenager Masa Vukotic in a Doncaster park in 2015.Like Meagher’s death, Vukotic’s murder was also mobilised by the media – and one could argue, by authorities – to contain women, to further a narrative that reinforces the public/private gender binary. However, as Koskela reminds, the fact that some women are bold and confident shows that women are not only passively experiencing space but actively take part in producing it. They reclaim space for themselves, not only through single occasions such as ‘take back the night’ marches, but through everyday practices and routinized uses of space. (“Bold Walks” 316)These memoirs act as resistance, actively producing space through representation: to assert the right to the city, one must be bold, and reclaim space that is so often overlaid with stories of violence against women. As Koskela emphasises, this is only done through use of the space, “a way of de-mystifying it. If one does not use the space, … ‘the mental map’ of the place is filled with indirect descriptions, the image of it is constructed through media and the stories heard” (“Bold Walks” 308). Memoir can take back this image through stories told, demonstrating the personal connection to public space. Koskela writes that, “walking on the street can be seen as a political act: women ‘write themselves onto the street’” (“Urban Space in Plural” 263). ReferencesAustralian Government. Department of Infrastructure and Transport. State of Australian Cities 2013. 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