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Harris, Richard A., Fabrizio Anniballi i John W. Austin. "Adult Intestinal Toxemia Botulism". Toxins 12, nr 2 (24.01.2020): 81. http://dx.doi.org/10.3390/toxins12020081.
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Intoxication with botulinum neurotoxin can occur through various routes. Foodborne botulism results after consumption of food in which botulinum neurotoxin-producing clostridia (i.e., Clostridium botulinum or strains of Clostridium butyricum type E or Clostridium baratii type F) have replicated and produced botulinum neurotoxin. Infection of a wound with C. botulinum and in situ production of botulinum neurotoxin leads to wound botulism. Colonization of the intestine by neurotoxigenic clostridia, with consequent production of botulinum toxin in the intestine, leads to intestinal toxemia botulism. When this occurs in an infant, it is referred to as infant botulism, whereas in adults or children over 1 year of age, it is intestinal colonization botulism. Predisposing factors for intestinal colonization in children or adults include previous bowel or gastric surgery, anatomical bowel abnormalities, Crohn’s disease, inflammatory bowel disease, antimicrobial therapy, or foodborne botulism. Intestinal colonization botulism is confirmed by detection of botulinum toxin in serum and/or stool, or isolation of neurotoxigenic clostridia from the stool, without finding a toxic food. Shedding of neurotoxigenic clostridia in the stool may occur for a period of several weeks. Adult intestinal botulism occurs as isolated cases, and may go undiagnosed, contributing to the low reported incidence of this rare disease.
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Nguyen, Duc, Thu Nguyen i Huu Nguyen. "Investigation of botulism in free-range ducks farming in the Mekong Delta, Vietnam". Open Veterinary Journal 12, nr 5 (2022): 632. http://dx.doi.org/10.5455/ovj.2022.v12.i5.7.
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Background: One of the most common diseases in free-range ducks in the Mekong Delta is “botulism”. Botulism is a poultry disease caused by botulinum exotoxin of Clostridium botulinum. Aim: The purpose of the investigation was to evaluate the prevalence of botulism in free-range ducks in the Mekong Delta and the risk of infection by determining the presence of Clostridium botulinum in the farming environment. Methods: Research on 200 duck flocks with 187050 individuals raised freely in the fields in the provinces of the Mekong Delta including An Giang, Can Tho, Hau Giang, and Kien Giang. The ducks were diagnosed with botulism based on clinical symptoms. To demonstrate the presence of botulinum neurotoxins and identify serotype, samples of serum and/or gut were analyzed by mouse bioassay. Samples of soil (n=600), water (n=600), crabs (n=216), and snails (n=400) were taken from the grazing regions for Clostridium botulinum analysis by PCR assay. Results: There were 1.19% (2235/187050) free-range ducks in the Mekong Delta positive for botulism. Clinical symptoms of botulism including limberneck, drooping eyelids - enlarged pupils, and leg paralysis were prevalent across free-range ducks, with the frequency of 87.92% (1965/2235), 90.07% (2013/2235), and 79.78% (1783/2235), respectively. The lesions of pulmonary edema – hemorrhage, hemorrhagic liver, and gas-producing intestines were common, accounting for 86.19% (362/420), 95.48% (401/420), and 92.14% (387/420), respectively. Botulin toxin type C was found in a considerable number of serum samples, accounting for 40.48% (51/126). Meanwhile, the percentage of serum samples containing botulin toxin types E and D was 28.57% (36/126) and 25.40% (32/126), respectively. Clostridium botulinum was detected in the farming environment specifically 17.5% (105/600) in soil, 19.67% (118/600) in water, 8.33% (18/216) in crabs, and 3.00% (12/400) in snails. Conclusion: The free-range ducks in the Mekong Delta were at high risk of botulism because of the latent presence of Clostridium botulinum in the farming environment.
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Mubarik Ali i Norina Jabeen. "Botulism a Major Risk in Animals After Flood in Pakistan; A Review". Indus Journal of Agriculture and Biology 1, nr 1 (31.12.2022): 1–7. http://dx.doi.org/10.59075/ijab.v1i1.139.
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Flooding has affected and will likely continue to alter the occurrence, distribution, and prevalence of animal diseases, including botulism, according to a growing body of evidence. The pathogen Clostridium botulinum is thought to be one of several species that can produce the A–H-coded botulinum toxins. These toxins (BoNT) are thought to be the most harmful elements found in nature. The poison hits nerves that are firing more frequently, which causes the pattern of damage. The toxin specifically affects synapses and neuromuscular junctions by preventing the generation or release of acetylcholine there. The majority of animals who contract botulism die from it; it affects the breathing, chewing, and swallowing muscles as well as the diaphragm and intercostal muscles, leading to flaccid paralysis and respiratory arrest. The neurotoxins types C and D produced by the bacterium Clostridium botulinum in an animal or plant substance, during decomposition, are the cause of the condition in cattle. Failure of the respiratory system causes death. The toxin that causes botulism, also known as botulinus poisoning, is produced by the bacterium Clostridium botulinum. There are few available treatment options for the neuroparalytic disease botulism, which impacts the livestock business globally and has been documented in a number of nations.
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Franciosa, Giovanna, Manoocheher Pourshaban, Alessandro De Luca, Anna Buccino, Bruno Dallapiccola i Paolo Aureli. "Identification of Type A, B, E, and F Botulinum Neurotoxin Genes and of Botulinum Neurotoxigenic Clostridia by Denaturing High-Performance Liquid Chromatography". Applied and Environmental Microbiology 70, nr 7 (lipiec 2004): 4170–76. http://dx.doi.org/10.1128/aem.70.7.4170-4176.2004.
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ABSTRACT Denaturing high-performance liquid chromatography (DHPLC) is a recently developed technique for rapid screening of nucleotide polymorphisms in PCR products. We used this technique for the identification of type A, B, E, and F botulinum neurotoxin genes. PCR products amplified from a conserved region of the type A, B, E, and F botulinum toxin genes from Clostridium botulinum, neurotoxigenic C. butyricum type E, and C. baratii type F strains were subjected to both DHPLC analysis and sequencing. Unique DHPLC peak profiles were obtained with each different type of botulinum toxin gene fragment, consistent with nucleotide differences observed in the related sequences. We then evaluated the ability of this technique to identify botulinal neurotoxigenic organisms at the genus and species level. A specific short region of the 16S rRNA gene which contains genus-specific and in some cases species-specific heterogeneity was amplified from botulinum neurotoxigenic clostridia and from different food-borne pathogens and subjected to DHPLC analysis. Different peak profiles were obtained for each genus and species, demonstrating that the technique could be a reliable alternative to sequencing for the rapid identification of food-borne pathogens, specifically of botulinal neurotoxigenic clostridia most frequently implicated in human botulism.
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Popoff, Michel R., i Holger Brüggemann. "Regulatory Networks Controlling Neurotoxin Synthesis in Clostridium botulinum and Clostridium tetani". Toxins 14, nr 6 (24.05.2022): 364. http://dx.doi.org/10.3390/toxins14060364.
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Clostridium botulinum and Clostridium tetani are Gram-positive, spore-forming, and anaerobic bacteria that produce the most potent neurotoxins, botulinum toxin (BoNT) and tetanus toxin (TeNT), responsible for flaccid and spastic paralysis, respectively. The main habitat of these toxigenic bacteria is the environment (soil, sediments, cadavers, decayed plants, intestinal content of healthy carrier animals). C. botulinum can grow and produce BoNT in food, leading to food-borne botulism, and in some circumstances, C. botulinum can colonize the intestinal tract and induce infant botulism or adult intestinal toxemia botulism. More rarely, C. botulinum colonizes wounds, whereas tetanus is always a result of wound contamination by C. tetani. The synthesis of neurotoxins is strictly regulated by complex regulatory networks. The highest levels of neurotoxins are produced at the end of the exponential growth and in the early stationary growth phase. Both microorganisms, except C. botulinum E, share an alternative sigma factor, BotR and TetR, respectively, the genes of which are located upstream of the neurotoxin genes. These factors are essential for neurotoxin gene expression. C. botulinum and C. tetani share also a two-component system (TCS) that negatively regulates neurotoxin synthesis, but each microorganism uses additional distinct sets of TCSs. Neurotoxin synthesis is interlocked with the general metabolism, and CodY, a master regulator of metabolism in Gram-positive bacteria, is involved in both clostridial species. The environmental and nutritional factors controlling neurotoxin synthesis are still poorly understood. The transition from amino acid to peptide metabolism seems to be an important factor. Moreover, a small non-coding RNA in C. tetani, and quorum-sensing systems in C. botulinum and possibly in C. tetani, also control toxin synthesis. However, both species use also distinct regulatory pathways; this reflects the adaptation of C. botulinum and C. tetani to different ecological niches.
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Lindström, Miia, i Hannu Korkeala. "Laboratory Diagnostics of Botulism". Clinical Microbiology Reviews 19, nr 2 (kwiecień 2006): 298–314. http://dx.doi.org/10.1128/cmr.19.2.298-314.2006.
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SUMMARY Botulism is a potentially lethal paralytic disease caused by botulinum neurotoxin. Human pathogenic neurotoxins of types A, B, E, and F are produced by a diverse group of anaerobic spore-forming bacteria, including Clostridium botulinum groups I and II, Clostridium butyricum, and Clostridium baratii. The routine laboratory diagnostics of botulism is based on the detection of botulinum neurotoxin in the patient. Detection of toxin-producing clostridia in the patient and/or the vehicle confirms the diagnosis. The neurotoxin detection is based on the mouse lethality assay. Sensitive and rapid in vitro assays have been developed, but they have not yet been appropriately validated on clinical and food matrices. Culture methods for C. botulinum are poorly developed, and efficient isolation and identification tools are lacking. Molecular techniques targeted to the neurotoxin genes are ideal for the detection and identification of C. botulinum, but they do not detect biologically active neurotoxin and should not be used alone. Apart from rapid diagnosis, the laboratory diagnostics of botulism should aim at increasing our understanding of the epidemiology and prevention of the disease. Therefore, the toxin-producing organisms should be routinely isolated from the patient and the vehicle. The physiological group and genetic traits of the isolates should be determined.
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Long, Sharon C., i Tiffany Tauscher. "Watershed issues associated with Clostridium botulinum: A literature review". Journal of Water and Health 4, nr 3 (1.04.2006): 277–88. http://dx.doi.org/10.2166/wh.2006.016b.
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Botulism the disease, the related organism (Clostridium botulinum) and toxin have gained renewed attention in these times of heightened homeland security and bioterrorism preparedness. Since C. botulinum is ubiquitous in nature, botulism outbreaks resulting from environmental exposure can be of concern to watershed managers and drinking water utilities. This paper reviews aspects of naturally occurring C. botulinum in light of concerns for source water watersheds. Information regarding sources and occurrence of botulism, C. botulinum and botulism toxins are discussed. Ecology and physiology of environmental C. botulinum and cycles of disease are reviewed. Finally, the effectiveness of water treatment and disinfection measures is discussed.
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Lanci, Aliai, Riccardo Rinnovati, Fabrizio Anniballi, Bruna Auricchio, Concetta Scalfaro, Marika Menchetti, Alessandro Spadari i Jole Mariella. "The First Case of Botulism in a Donkey". Veterinary Sciences 6, nr 2 (15.05.2019): 43. http://dx.doi.org/10.3390/vetsci6020043.
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Botulism, a severe neuroparalytic disease that can affect humans, all warm-blooded animals, and some fishes, is caused by exotoxins produced by ubiquitous, obligate anaerobic, spore-forming bacteria belonging to the genus Clostridium and named botulinum neurotoxin (BoNT)-producing clostridia. This report presents the case of a 3-year-old donkey mare referred for progressive and worsening dysphagia of four days’ duration. Her voluntary effort in eating and drinking was conserved, and she was able to slow chew without swallowing. A complete neurological examination was performed, and botulism was strongly suspected. The ability to swallow feed and water returned on the tenth day of hospitalization and improved progressively. The jenny was discharged from the hospital after fifteen days. During the hospitalization, the Italian National Reference Centre for Botulism confirmed the diagnosis: mare’s feces were positive for BoNT/B and Clostridium botulinum type B.
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Pohanka, Miroslav. "Botulinum Toxin as a Biological Warfare Agent: Poisoning, Diagnosis and Countermeasures". Mini-Reviews in Medicinal Chemistry 20, nr 10 (27.05.2020): 865–74. http://dx.doi.org/10.2174/1389557520666200228105312.
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Botulinum toxin is a neurotoxin produced by Clostridium botulinum and some other relative species. It causes a lethal disease called botulism. It can enter the body via infections by Clostridium (e.g. wound and children botulism) or by direct contact with the toxin or eating contaminated food (food-borne botulism). Botulinum toxin is also considered as a relevant biological warfare agent with an expected high number of causalities when misused for bioterrorist or military purposes. The current paper surveys the actual knowledge about botulinum toxin pathogenesis, the manifestation of poisoning, and current trends in diagnostics and therapeutics. Relevant and recent literature is summarized in this paper.
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HAUSCHILD, A. H. W., R. HILSHEIMER, K. F. WEISS i R. B. BURKE. "Clostridium Botulinum in Honey, Syrups and Dry Infant Cereals". Journal of Food Protection 51, nr 11 (1.11.1988): 892–94. http://dx.doi.org/10.4315/0362-028x-51.11.892.
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A total of 150 honey, 43 syrup and 40 dry cereal samples were analyzed for Clostridium botulinum spores, each in triplicate quantities of 25 g. The foods were sampled randomly, except for two lots of honey which were potentially associated with illness. Botulinal spores were detected in a sample of honey associated with infant botulism and in a single sample of rice cereal.
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Tjampakasari, Conny Riana, i Rifdah Hanifah. "Bakteri Anaerob Clostridium botulinum dan Toksin yang Dihasilkannya". Cermin Dunia Kedokteran 49, nr 5 (2.05.2022): 260–64. http://dx.doi.org/10.55175/cdk.v49i5.230.
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C. botulinum adalah salah satu bakteri paling patogen karena dapat menghasilkan botulinum neurotoxin (BoNT) yang mematikan. Terdapat 3 jenis botulisme, yaitu botulisme keracunan makanan, botulisme inhalasi, dan botulisme luka. Meskipun kejadian botulisme jarang, namun harus diwaspadai karena cukup fatal. Sebagian besar kasus botulisme pada manusia disebabkan oleh makanan kaleng yang dipersiapkan di rumah. Isolasi dan identifikasi C. botulinum dapat dilakukan dengan pemeriksaan pewarnaan Gram, kultur, dan identifikasi, sedangkan deteksi toksin dapat menggunakan metode mouse lethality assay, non-lethal mouse assay, dan metode imunologi. Pendekatan molekuler dilakukan melalui uji polymerase chain reaction (PCR) untuk deteksi jenis toksin. Pencegahan botulisme dilakukan dengan teknik penanganan makanan yang tepat. Pemanasan yang memadai dapat membunuh spora bakteri, selain itu segera mengonsumsi makanan yang telah dimasak dapat mencegah C. botulinum bertumbuh.
C. botulinum is one of the most pathogenic bacteria because it can produce deadly botulinum neutotoxin (BoNT). There are 3 types of botulism: botulism poisoning, inhalation botulism, and wound botulism. Although the incidence of botulism is rare, the impact is quite fatal. Most cases of botulism in human are caused by canned food prepared at home. Isolation and identification of C. botulinum can be done by Gram staining, culture, and identification, while the detection of toxins can use the mouse lethality assay, non-lethal mouse assay, and immunological methods. Molecular approach can be done through polymerase chain reaction (PCR) examination to detect the toxin type. Prevention can be applied with proper food handling techniques. Adequate heating can kill bacterial spores, and direct consumption can prevent proliferation of C. botulinum.
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Raphael, Brian H., Mallory J. Choudoir, Carolina Lúquez, Rafael Fernández i Susan E. Maslanka. "Sequence Diversity of Genes Encoding Botulinum Neurotoxin Type F". Applied and Environmental Microbiology 76, nr 14 (28.05.2010): 4805–12. http://dx.doi.org/10.1128/aem.03109-09.
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ABSTRACT Botulism due to type F botulinum neurotoxin (BoNT/F) is rare (<1% of cases), and only a limited number of clostridial strains producing this toxin type have been isolated. As a result, analysis of the diversity of genes encoding BoNT/F has been challenging. In this study, the entire bont/F nucleotide sequences were determined from 33 type F botulinum toxin-producing clostridial strains isolated from environmental sources and botulism outbreak investigations. We examined proteolytic and nonproteolytic Clostridium botulinum type F strains, bivalent strains, including Bf and Af, and Clostridium baratii type F strains. Phylogenetic analysis revealed that the bont/F genes examined formed 7 subtypes (F1 to F7) and that the nucleotide sequence identities of these subtypes differed by up to 25%. The genes from proteolytic (group I) C. botulinum strains formed subtypes F1 through F5, while the genes from nonproteolytic (group II) C. botulinum strains formed subtype F6. Subtype F7 was composed exclusively of bont/F genes from C. baratii strains. The region of the bont/F5 gene encoding the neurotoxin light chain was found to be highly divergent compared to the other subtypes. Although the bont/F5 nucleotide sequences were found to be identical in strains harboring this gene, the gene located directly upstream (ntnh/F) demonstrated sequence variation among representative strains of this subtype. These results demonstrate that extensive nucleotide diversity exists among genes encoding type F neurotoxins from strains with different phylogenetic backgrounds and from various geographical sources.
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Uzal, Francisco A., Mauricio A. Navarro, Javier Asin i Eileen E. Henderson. "Clostridial Diseases of Horses: A Review". Vaccines 10, nr 2 (17.02.2022): 318. http://dx.doi.org/10.3390/vaccines10020318.
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The clostridial diseases of horses can be divided into three major groups: enteric/enterotoxic, histotoxic, and neurotoxic. The main enteric/enterotoxic diseases include those produced by Clostridium perfringens type C and Clostridioides difficile, both of which are characterized by enterocolitis. The main histotoxic diseases are gas gangrene, Tyzzer disease, and infectious necrotic hepatitis. Gas gangrene is produced by one or more of the following microorganisms: C. perfringens type A, Clostridium septicum, Paeniclostridium sordellii, and Clostridium novyi type A, and it is characterized by necrotizing cellulitis and/or myositis. Tyzzer disease is produced by Clostridium piliforme and is mainly characterized by multifocal necrotizing hepatitis. Infectious necrotic hepatitis is produced by Clostridium novyi type B and is characterized by focal necrotizing hepatitis. The main neurotoxic clostridial diseases are tetanus and botulism, which are produced by Clostridium tetani and Clostridium botulinum, respectively. Tetanus is characterized by spastic paralysis and botulism by flaccid paralysis. Neither disease present with specific gross or microscopic lesions. The pathogenesis of clostridial diseases involves the production of toxins. Confirming a diagnosis of some of the clostridial diseases of horses is sometimes difficult, mainly because some agents can be present in tissues of normal animals. This paper reviews the main clostridial diseases of horses.
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Tjampakasari, Conny Riana, i Rifdah Hanifah. "Bakteri Anaerob Clostridium botulinum dan Toksin yang Dihasilkannya". Cermin Dunia Kedokteran 49, nr 5 (27.04.2022): 260. http://dx.doi.org/10.55175/cdk.v49i5.1849.
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<p>C. botulinum adalah salah satu bakteri paling patogen karena dapat menghasilkan botulinum neurotoxin (BoNT) yang mematikan. Terdapat 3 jenis botulisme, yaitu botulisme keracunan makanan, botulisme inhalasi, dan botulisme luka. Meskipun kejadian botulisme jarang, namun harus diwaspadai karena cukup fatal. Sebagian besar kasus botulisme pada manusia disebabkan oleh makanan kaleng yang dipersiapkan di rumah. Isolasi dan identifikasi C. botulinum dapat dilakukan dengan pemeriksaan pewarnaan Gram, kultur, dan identifikasi, sedangkan deteksi toksin dapat menggunakan metode mouse lethality assay, non-lethal mouse<br />assay, dan metode imunologi. Pendekatan molekuler dilakukan melalui uji polymerase chain reaction (PCR) untuk deteksi jenis toksin. Pencegahan botulisme dilakukan dengan teknik penanganan makanan yang tepat. Pemanasan yang memadai dapat membunuh spora bakteri, selain itu segera mengonsumsi makanan yang telah dimasak dapat mencegah C. botulinum bertumbuh.</p><p> </p><p>C. botulinum is one of the most pathogenic bacteria because it can produce deadly botulinum neutotoxin (BoNT). There are 3 types of botulism: botulism poisoning, inhalation botulism, and wound botulism. Although the incidence of botulism is rare, the impact is quite fatal. Most cases of botulism in human are caused by canned food prepared at home. Isolation and identification of C. botulinum can be done by Gram staining, culture, and identification, while the detection of toxins can use the mouse lethality assay, non-lethal mouse assay, and immunological methods. Molecular approach can be done through polymerase chain reaction<br />(PCR) examination to detect the toxin type. Prevention can be applied with proper food handling techniques. Adequate heating can kill bacterial spores, and direct consumption can prevent proliferation of C. botulinum.</p>
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Brunt, Jason, Arnoud H. M. van Vliet, Andrew T. Carter, Sandra C. Stringer, Corinne Amar, Kathie A. Grant, Gauri Godbole i Michael W. Peck. "Diversity of the Genomes and Neurotoxins of Strains of Clostridium botulinum Group I and Clostridium sporogenes Associated with Foodborne, Infant and Wound Botulism". Toxins 12, nr 9 (11.09.2020): 586. http://dx.doi.org/10.3390/toxins12090586.
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Clostridium botulinum Group I and Clostridium sporogenes are closely related bacteria responsible for foodborne, infant and wound botulism. A comparative genomic study with 556 highly diverse strains of C. botulinum Group I and C. sporogenes (including 417 newly sequenced strains) has been carried out to characterise the genetic diversity and spread of these bacteria and their neurotoxin genes. Core genome single-nucleotide polymorphism (SNP) analysis revealed two major lineages; C. botulinum Group I (most strains possessed botulinum neurotoxin gene(s) of types A, B and/or F) and C. sporogenes (some strains possessed a type B botulinum neurotoxin gene). Both lineages contained strains responsible for foodborne, infant and wound botulism. A new C. sporogenes cluster was identified that included five strains with a gene encoding botulinum neurotoxin sub-type B1. There was significant evidence of horizontal transfer of botulinum neurotoxin genes between distantly related bacteria. Population structure/diversity have been characterised, and novel associations discovered between whole genome lineage, botulinum neurotoxin sub-type variant, epidemiological links to foodborne, infant and wound botulism, and geographic origin. The impact of genomic and physiological variability on the botulism risk has been assessed. The genome sequences are a valuable resource for future research (e.g., pathogen biology, evolution of C. botulinum and its neurotoxin genes, improved pathogen detection and discrimination), and support enhanced risk assessments and the prevention of botulism.
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FRANCIOSA, GIOVANNA, MANOOCHEHER POURSHABAN, MONICA GIANFRANCESCHI, ANTONIETTA GATTUSO, LUCIA FENICIA, ANNA MARIA FERRINI, VERUSCKA MANNONI, GREGORIO DE LUCA i PAOLO AURELI. "Clostridium botulinum Spores and Toxin in Mascarpone Cheese and Other Milk Products". Journal of Food Protection 62, nr 8 (1.08.1999): 867–71. http://dx.doi.org/10.4315/0362-028x-62.8.867.
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A total of 1,017 mascarpone cheese samples, collected at retail, were analyzed for Clostridium botulinum spores and toxin, aerobic mesophilic spore counts, as well as pH, aw (water activity), and Eh (oxidation–reduction potential). In addition 260 samples from other dairy products were also analyzed for spores and botulinum toxin. Experiments were carried out on naturally and artificially contaminated mascarpone to investigate the influence of different temperature conditions on toxin production by C. botulinum. Three hundred and thirty-one samples (32.5%) of mascarpone were positive for botulinal spores, and 7 (0.8%) of the 878 samples produced at the plant involved in an outbreak of foodborne botulism also contained toxin type A. The chemical–physical parameters (pH, aw, Eh) of all samples were compatible with C. botulinum growth and toxinogenesis. Of the other milk products, 2.7% were positive for C. botulinum spores. Growth and toxin formation occurred in naturally and experimentally contaminated mascarpone samples after 3 and 4 days of incubation at 28°C, respectively.
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Bradshaw, Marite, Kristin M. Marshall, John T. Heap, William H. Tepp, Nigel P. Minton i Eric A. Johnson. "Construction of a Nontoxigenic Clostridium botulinum Strain for Food Challenge Studies". Applied and Environmental Microbiology 76, nr 2 (20.11.2009): 387–93. http://dx.doi.org/10.1128/aem.02005-09.
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ABSTRACT Clostridium botulinum produces the most poisonous natural toxin known and is a perennial concern to the food industry and to regulatory agencies due to the potential threat of food-borne botulism. To ensure the botulinal safety of foods, rigorous food challenge testing to validate food-processing conditions and food formulations has been routinely performed. Detection of the botulinum neurotoxin is performed by using a mouse bioassay and/or in vitro assays. There has been considerable interest by the food industry and regulatory agencies in minimizing or even replacing the use of animals in these challenge studies. In addition, due to stringent select-agent regulations, the testing of various foods using toxigenic C. botulinum strains requires facilities and personnel that are certified for work with this organism. For this purpose we propose to generate sets of nontoxigenic C. botulinum strains from proteolytic and nonproteolytic groups that differ from the wild-type strains only by their inability to produce botulinum neurotoxin. In this initial study we describe the generation of a nontoxigenic mutant of C. botulinum strain 62A using the ClosTron mutagenesis system by inserting a group II intron into the botulinum neurotoxin type A gene (bont/A). The mutant clones were nontoxigenic as determined by Western blots and mouse bioassays but showed physiological characteristics, including growth properties and sporulation, that were similar to those of the parent strain in laboratory media. Additional studies will be required to evaluate comparable characteristics in various food matrices. The availability of suitable nontoxigenic C. botulinum strains for food challenge studies will be beneficial for enhancing the botulinal safety of foods as well as increasing the biosafety of workers and may eliminate the use of laboratory animals.
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Hung, Le Quoc, Vo Ngoc Anh Tho, Do Thi Ngoc Khanh, Vo Thi Thanh Hien, Jeremy N. Day, Nguyen Ngoc Sang, Hua Thoai Tam, Ho Thi Chi Thanh i Nguyen Thi Thuy Ngan. "Suspected botulism outbreak after the consumption of vegetarian pâté in the south of Viet Nam". Wellcome Open Research 5 (18.06.2021): 257. http://dx.doi.org/10.12688/wellcomeopenres.16372.4.
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Botulism and other botulinum neurotoxins-producing clostridia are potentially life-threatening diseases caused by toxins produced by Clostridium botulinum. Here we reported a case series of six patients who presented with botulism following ingestion of commercially made pâté. The key features of presentation were acute onset of bilateral cranial nerve palsies and symmetrical descending weakness in the absence of fever resulting in the need for mechanical ventilation in all six patients. The clinical diagnosis of botulism was confirmed through the identification of C. botulinum from the suspected food source. Given that botulinum antitoxin was not available in Vietnam at the time, and their severe status, all patients received a trial of plasma exchange therapy, but no clear benefit was seen. Due to its rarity, diagnosing botulism is a challenge, demanding high clinical suspicion. Successful outcomes depend upon early recognition and rapid initiation of specific treatment with botulinum antitoxin. There is a need to improve global access to antitoxin. These cases, the first in Viet Nam, serve as a reminder of the need to maintain the highest possible food hygiene and preservation practices.
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Netthisinghe, Annesly, Paul Woosley, Naomi Rowland, Todd Willian, Becky Gilfillen i Karamat Sistani. "Alfalfa Forage Production and Nutritive Value, Fermentation Characteristics and Hygienic Quality of Ensilage, and Soil Properties after Broiler Litter Amendment". Agronomy 11, nr 4 (7.04.2021): 701. http://dx.doi.org/10.3390/agronomy11040701.
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Recycling broiler litter (BL) nutrients is an important strategy for sustainable forage production. However, BL can contain Clostridia bacteria that can contaminate forages at harvest, resulting in poor ensilage quality and botulism-related animal health risks. A better understanding of the effects of BL amendment on alfalfa (Medicago sativa L.) production and ensiling is beneficial for promoting manure-based alfalfa production. This 2-year study examined the effects of high-level BL (HBL) at 112 kg N ha−1 and low-level (LBL) at 56 kg N ha−1 on alfalfa forage production, fermentation characteristics, and Clostridium botulinum concentrations in silage and haylage produced from 350 g dry matter (DM) kg−1 forage and 500 g DM kg−1 forage respectively, and soil characteristics compared to a control treatment (CT). Results showed that the application of BL did not affect forage production (12.8–13.1 MG ha−1) and nutritive value. The alfalfa produced high forage yield with superior ensilabilty in the second year. The BL application increased soil NH4-N, Ca, Fe, and B, but did not affect fermentation characteristics or Clostridium botulinum concentrations in ensilage. Silage had superior fermentation quality, and Clostridium botulinum concentration was found to be higher than in haylage. Broiler litter fertilization for alfalfa is environmentally safe and has forge production, ensilage fermentation quality, and botulism risks similar to CT.
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Maikanov, Balgabay, Raikhan Mustafina, Laura Auteleyeva, Jan Wiśniewski, Krzysztof Anusz, Tomasz Grenda, Krzysztof Kwiatek, Magdalena Goldsztejn i Magdalena Grabczak. "Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples". Toxins 11, nr 8 (13.08.2019): 472. http://dx.doi.org/10.3390/toxins11080472.
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The aim of this study was to assess occurrence of Clostridium botulinum and Clostridium perfringens in honey samples from Kazakhstan. Analyses were carried out using a set of PCR methods for identification of anaerobic bacteria, and detection of toxin genes of C. botulinum and C. perfringens. Among 197 samples, C. botulinum was noticed in only one (0.5%). The isolated strain of this pathogen showed the presence of the bont/A and ntnh genes. C. perfringens strains were isolated from 18 (9%) samples, and mPCR (multiplex PCR) analysis led to them all being classified as toxin type A with the ability to produce α toxin. Sequence analysis of 16S rDNA genes showed occurrence in 4 samples of other anaerobes related to C. botulinum, which were C. sporogenes and C. beijerinckii strains. C. botulinum prevalence in honey samples from Kazakhstan in comparison to the prevalence in samples collected from the other regions seems to be less. The highest prevalence of Clostridium sp. was noticed in the East Kazakhstan province. Our study is the first survey on BoNT-producing clostridia and C. perfringens prevalence in Kazakh honey.
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Fenicia, Lucia, Fabrizio Anniballi, Dario De Medici, Elisabetta Delibato i Paolo Aureli. "SYBR Green Real-Time PCR Method To Detect Clostridium botulinum Type A". Applied and Environmental Microbiology 73, nr 9 (16.03.2007): 2891–96. http://dx.doi.org/10.1128/aem.02234-06.
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ABSTRACT Botulinum toxins (BoNTs) are classically produced by Clostridium botulinum but rarely also from neurotoxigenic strains of Clostridium baratii and Clostridium butyricum. BoNT type A (BoNT/A), BoNT/B, BoNT/E, and very rarely BoNT/F are mainly responsible for human botulism. Standard microbiological methods take into consideration only the detection of C. botulinum. The presumptive identification of the toxigenic strains together with the typing of BoNT has to be performed by mouse bioassay. The development of PCR-based methods for the detection and typing of BoNT-producing clostridia would be an ideal alternative to the mouse bioassay. The objective of this study was to develop a rapid and robust real-time PCR method for detecting C. botulinum type A. Four different techniques for the extraction and purification of DNA from cultured samples were initially compared. Of the techniques used, Chelex 100, DNeasy tissue kit, InstaGene matrix DNA, and boiling, the boiling technique was significantly less efficient than the other three. These did not give statistically different results, and Chelex 100 was chosen because it was less expensive than the others. In order to eliminate any false-negative results, an internal amplification control was synthesized and included in the amplification mixture according to ISO 22174. The specificity of the method was tested against 75 strains of C. botulinum type A, 4 strains of C. botulinum type Ab, and 101 nontarget strains. The detection limit of the reaction was less than 6 × 101 copies of C. botulinum type A DNA. The robustness of the method was confirmed using naturally contaminated stool specimens to evaluate the tolerance of inhibitor substances. SYBR green real-time PCR showed very high specificity for the detection of C. botulinum types A and Ab (inclusivity and exclusivity, 100%).
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J. Pflug, Irving. "Report: Controlling Clostridium Botulinum In Heat-Preserved Food". Nutrition and Food Processing 1, nr 1 (23.05.2018): 01–03. http://dx.doi.org/10.31579/2637-8914/003.
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The purpose of this report is to explain, in easy to understand outline form, the basic principles of controlling Clostridium botulinum in heat-preserved food. There are three major parts to the story: (1) Truths regarding the Clostridium botulinum problem, (2) Controlling the Clostridium botulinum hazard; and (3) Estimating the probability of process failure. The later includes errors that may occur in the process design area and errors that may occur in the process delivery area. In the report, "Science, Practice and Human Errors in Controlling Clostridium botulinum in Heat-Preserved Food in Hermetic Containers," by Pflug, Irving J., Journal of Food Protection, 73(5):993-1002 (2010), the important story of Clostridium botulinum has been told in detail, but the important results have been hidden by the author's syntax. In this manuscript, "Controlling Clostridium botulinum in Heat-Preserved Food," we hope to make available the details of Clostridium botulinum control. The purpose of this report is to explain, in easy to understand, outline form, the basic principles of controlling Clostridium botulinum in heat-preserved food. Quite simply, there are three major aspects to Clostridium botulinum control: (1) Facts regarding the Clostridium botulinum organism and its spores; (2) How to control the Clostridium botulinum hazard; and (3) Estimating the probability of process failure.
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Grenda, Tomasz, Aleksandra Jarosz, Magdalena Sapała, Karol Stasiak, Anna Grenda, Piotr Domaradzki i Krzysztof Kwiatek. "Molecular Diversity of BoNT-Producing Clostridia—A Still-Emerging and Challenging Problem". Diversity 15, nr 3 (9.03.2023): 392. http://dx.doi.org/10.3390/d15030392.
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The diversity of BoNT-producing Clostridia is still a worrying problem for specialists who explore the evolutionary and taxonomic diversity of C. botulinum. It is also a problem for epidemiologists and laboratory staff conducting investigations into foodborne botulism in humans and animals, because their genetic and phenotypic heterogeneity cause complications in choosing the proper analytical tools and in reliably interpreting results. Botulinum neurotoxins (BoNTs) are produced by several bacterial groups that meet all the criteria of distinct species. Despite this, the historical designation of C. botulinum as the one species that produces botulinum toxins is still exploited. New genetic tools such as whole-genome sequencing (WGS) indicate horizontal gene transfer and the occurrence of botulinum gene clusters that are not limited only to Clostridium spp., but also to Gram-negative aerobic species. The literature data regarding the mentioned heterogeneity of BoNT-producing Clostridia indicate the requirement to reclassify C. botulinum species and other microorganisms able to produce BoNTs or possessing botulinum-like gene clusters. The aim of this study was to present the problem of the diversity of BoNT-producing Clostridia over time and new trends toward obtaining a reliable classification of these microorganisms, based on a complex review of the literature.
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Wojtacka, Joanna, Beata Wysok, Zbigniew Lipiński, Małgorzata Gomółka-Pawlicka, Helena Rybak-Chmielewska i Agnieszka Wiszniewska-Łaszczych. "Clostridium botulinum Spores Found in Honey from Small Apiaries in Poland". Journal of Apicultural Science 60, nr 2 (1.12.2016): 89–100. http://dx.doi.org/10.1515/jas-2016-0020.
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Abstract A total of 102 honey samples collected from small apiaries (≤ 20 hives) in Poland were analysed for the presence of Clostridium botulinum spores. The samples were prepared using the dilution centrifugation method and cultured in parallel in cooked meat medium (CMM) and tripticase peptone glucose yeast (TPGY) enrichment broths. Identification of toxin types A, B, and E of Clostridium botulinum strains was performed with the use of the multiplex PCR method. Positive samples were also subjected to quantitative analysis with the use of Clostridium botulinum Isolation Agar Base (CBAB). The prevalence analysis showed 22 (21.6%) samples contaminated with C. botulinum spores. The major serotype detected was botulin neurotoxin type A – 16 (72.7%) whereas type B was found in 3 (13.6%) honey samples and type E also only in 3 (13.6%) honey samples. Dual-toxin-producing strains were noted. The average quantity of spores in PCR - C. botulinum positive samples was 190 in 1 gram of honey.
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Fillo, Silvia, Francesco Giordani, Elena Tonon, Ilenia Drigo, Anna Anselmo, Antonella Fortunato, Florigio Lista i Luca Bano. "Extensive Genome Exploration of Clostridium botulinum Group III Field Strains". Microorganisms 9, nr 11 (13.11.2021): 2347. http://dx.doi.org/10.3390/microorganisms9112347.
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In animals, botulism is commonly sustained by botulinum neurotoxin C, D or their mosaic variants, which are produced by anaerobic bacteria included in Clostridium botulinum group III. In this study, a WGS has been applied to a large collection of C. botulinum group III field strains in order to expand the knowledge on these BoNT-producing Clostridia and to evaluate the potentiality of this method for epidemiological investigations. Sixty field strains were submitted to WGS, and the results were analyzed with respect to epidemiological information and compared to published sequences. The strains were isolated from biological or environmental samples collected in animal botulism outbreaks which occurred in Italy from 2007 to 2016. The new sequenced strains belonged to subspecific groups, some of which were already defined, while others were newly characterized, peculiar to Italian strains and contained genomic features not yet observed. This included, in particular, two new flicC types (VI and VII) and new plasmids which widen the known plasmidome of the species. The extensive genome exploration shown in this study improves the C. botulinum and related species classification scheme, enriching it with new strains of rare genotypes and permitting the highest grade of discrimination among strains for forensic and epidemiological applications.
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Morzywolek, Agnieszka, Magdalena Plotka, Anna-Karina Kaczorowska, Monika Szadkowska, Lukasz P. Kozlowski, Dariusz Wyrzykowski, Joanna Makowska i in. "Novel Lytic Enzyme of Prophage Origin from Clostridium botulinum E3 Strain Alaska E43 with Bactericidal Activity against Clostridial Cells". International Journal of Molecular Sciences 22, nr 17 (2.09.2021): 9536. http://dx.doi.org/10.3390/ijms22179536.
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Clostridium botulinum is a Gram-positive, anaerobic, spore-forming bacterium capable of producing botulinum toxin and responsible for botulism of humans and animals. Phage-encoded enzymes called endolysins, which can lyse bacteria when exposed externally, have potential as agents to combat bacteria of the genus Clostridium. Bioinformatics analysis revealed in the genomes of several Clostridium species genes encoding putative N-acetylmuramoyl-l-alanine amidases with anti-clostridial potential. One such enzyme, designated as LysB (224-aa), from the prophage of C. botulinum E3 strain Alaska E43 was chosen for further analysis. The recombinant 27,726 Da protein was expressed and purified from E. coli Tuner(DE3) with a yield of 37.5 mg per 1 L of cell culture. Size-exclusion chromatography and analytical ultracentrifugation experiments showed that the protein is dimeric in solution. Bioinformatics analysis and results of site-directed mutagenesis studies imply that five residues, namely H25, Y54, H126, S132, and C134, form the catalytic center of the enzyme. Twelve other residues, namely M13, H43, N47, G48, W49, A50, L73, A75, H76, Q78, N81, and Y182, were predicted to be involved in anchoring the protein to the lipoteichoic acid, a significant component of the Gram-positive bacterial cell wall. The LysB enzyme demonstrated lytic activity against bacteria belonging to the genera Clostridium, Bacillus, Staphylococcus, and Deinococcus, but did not lyse Gram-negative bacteria. Optimal lytic activity of LysB occurred between pH 4.0 and 7.5 in the absence of NaCl. This work presents the first characterization of an endolysin derived from a C. botulinum Group II prophage, which can potentially be used to control this important pathogen.
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Awsare, Sohun, David Chirikian i Forshing Lui. "Wound Botulism Caused by Botulinum Neurotoxin Type A in a Chronic Parenteral Drug Abuser". Case Reports in Neurology 12, nr 3 (12.11.2020): 422–27. http://dx.doi.org/10.1159/000510846.
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Botulism is an acute paralytic disease caused by botulinum neurotoxin (BoNT)-mediated inhibition of neurosignaling at the neuromuscular junction. BoNTs are produced by gram positive, anaerobic, spore-forming bacteria from the genus <i>Clostridium,</i>most commonly<i> Clostridium botulinum</i>. Over the last decade, a previously uncommon form of botulism, wound botulism, has increased in prevalence possibly due to the rise in parenteral drug abuse. A 53-year-old patient with a history of drug abuse presents to a rural emergency department with rapidly progressing lower extremity weakness over the past few days. He reports a recent heroin injection into right buttock and diffuse skin-popping scarring was observed throughout. The patient was treated with heptavalent botulinum antitoxin obtained from the Center for Disease Control and Prevention (CDC). A right thigh abscess culture was positive for<i> Clostridium tertium</i>, a left hip abscess culture was positive for methicillin-susceptible <i>Staphylococcus aureus</i> (MSSA), and blood culture confirmed multi-microbial bacteremia caused by <i>Staphylococcus epidermidis</i> and <i>Streptococcus mitis</i>. Serum analysis was positive for BoNT type A from a suspected concurrent<i> Clostridium botulinum</i> infection as <i>C. tertium</i> is not known to produce BoNT type A. This case report highlights the importance of early antitoxin treatment for patients with suspected wound botulism.
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Hernandez-de Mezerville, Marcela, Mariela Rojas-Solano, Alfonso Gutierrez-Mata, Laura Hernandez-Con i Rolando Ulloa-Gutierrez. "Infant botulism in Costa Rica: first report from Central America". Journal of Infection in Developing Countries 8, nr 01 (15.01.2014): 123–25. http://dx.doi.org/10.3855/jidc.3594.
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Introduction: Clostridium botulinum is known to cause descending paralysis in infants throughout the world. Methodology: The subject of this study was a three-month-old Costa Rican boy who was hospitalized because of poor suction and feeding, hypotonia, and constipation. Clinical history and physical examination findings suggested infant botulism. Samples were sent to the Winnipeg Public Health Laboratory, where Clostridium botulinum toxin A was identified by PCR and culture from the stools, making this the first report of infant botulism in Central America. Conclusions: Although infant botulism is a known disease, the limitations in identifying it in Central America contributes to the misdiagnosis and under-reporting of this disease.
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Rasetti-Escargueil, Christine, Emmanuel Lemichez i Michel R. Popoff. "Public Health Risk Associated with Botulism as Foodborne Zoonoses". Toxins 12, nr 1 (30.12.2019): 17. http://dx.doi.org/10.3390/toxins12010017.
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Botulism is a rare but severe neurological disease in man and animals that is caused by botulinum neurotoxins (BoNTs) produced by Clostridium botulinum and atypical strains from other Clostridium and non-Clostridium species. BoNTs are divided into more than seven toxinotypes based on neutralization with specific corresponding antisera, and each toxinotype is subdivided into subtypes according to amino acid sequence variations. Animal species show variable sensitivity to the different BoNT toxinotypes. Thereby, naturally acquired animal botulism is mainly due to BoNT/C, D and the mosaic variants CD and DC, BoNT/CD being more prevalent in birds and BoNT/DC in cattle, whereas human botulism is more frequently in the types A, B and E, and to a lower extent, F. Botulism is not a contagious disease, since there is no direct transmission from diseased animals or man to a healthy subject. Botulism occurs via the environment, notably from food contaminated with C. botulinum spores and preserved in conditions favorable for C. botulinum growth and toxin production. The high prevalence of botulism types C, D and variants DC and CD in farmed and wild birds, and to a lower extent in cattle, raises the risk of transmission to human beings. However, human botulism is much rarer than animal botulism, and botulism types C and D are exceptional in humans. Only 15 cases or suspected cases of botulism type C and one outbreak of botulism type D have been reported in humans to date. In contrast, animal healthy carriers of C. botulinum group II, such as C. botulinum type E in fish of the northern hemisphere, and C. botulinum B4 in pigs, represent a more prevalent risk of botulism transmission to human subjects. Less common botulism types in animals but at risk of transmission to humans, can sporadically be observed, such as botulism type E in farmed chickens in France (1998–2002), botulism type B in cattle in The Netherlands (1977–1979), botulism types A and B in horses, or botulism type A in dairy cows (Egypt, 1976). In most cases, human and animal botulisms have distinct origins, and cross transmissions between animals and human beings are rather rare, accidental events. But, due to the severity of this disease, human and animal botulism requires a careful surveillance.
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SHARMA, SHASHI K., i RICHARD C. WHITING. "Methods for Detection of Clostridium botulinum Toxin in Foods†". Journal of Food Protection 68, nr 6 (1.06.2005): 1256–63. http://dx.doi.org/10.4315/0362-028x-68.6.1256.
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Botulism is a deadly disease caused by ingestion of the preformed neurotoxin produced from the anaerobic spore-forming bacteria Clostridium botulinum. Botulinum neurotoxins are the most poisonous toxins known and have been a concern in the food industry for a long time. Therefore, rapid identification of botulinum neurotoxin using molecular and biochemical techniques is an essential component in the establishment of coordinated laboratory response systems and is the focus of current research and development. Because of the extreme toxicity of botulinum neurotoxin, some confirmatory testing with the mouse bioassay is still necessary, but rapid methods capable of screening large numbers of samples are also needed. This review is focused on the development of several detection methods for botulinum neurotoxins in foods.
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Cortés-Sánchez, Alejandro De Jesús. "About Clostridium botulinum, Fish and Tilapia". Modern Applied Science 15, nr 3 (8.04.2021): 1. http://dx.doi.org/10.5539/mas.v15n3p1.
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Fish and products are considered a food of nutritional quality that constituents a part of the human diet, produced and commercialized worldwide. Tilapia is one of the main fish for aquaculture production destined for human consumption in different presentations: refrigerated, frozen, fillet, cured, canned, among others. Fish, in addition to being a highly nutritious food, is also sensitive to deterioration and contamination along the food chain, being able to be contaminated mainly by microorganisms that are casual agents of consumer illnesses. Clostridium botulinum and spores can contaminate foods such as fish and products whose germination, growth and generation of botulinum toxin puts the health of consumers at high risk of acquiring botulism disease, which is of importance in public health due to its incidence and high fatality rate. This review describes in a general way the aspects related to fish and tilapia, foodborne diseases such as botulism, the causal agent, in addition to sanitary regulation, control and prevention of contamination of food products to protect food safety, and consumer’s health.
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McGrath, S., J. S. G. Dooley i R. W. Haylock. "Quantification of Clostridium botulinumToxin Gene Expression by Competitive Reverse Transcription-PCR". Applied and Environmental Microbiology 66, nr 4 (1.04.2000): 1423–28. http://dx.doi.org/10.1128/aem.66.4.1423-1428.2000.
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ABSTRACT Clostridium botulinum produces a characteristic botulinum neurotoxin which can cause an often fatal neuroparalytic condition known as botulism. Although food-borne botulism is rare, critical screening by food companies is necessary to ensure that food products are safe. At present, the food industry assesses the risks of botulinum neurotoxin production by challenge testing to check any new food products and to check the efficacy of new storage regimes. Challenge testing involves artificial introduction of defined strains of microorganisms into food, and microbial growth and possible toxin production are then monitored. Botulinum toxin is normally analyzed by using the mouse bioassay. However, the mouse bioassay is expensive, slow, and politically sensitive because of animal rights issues. In this paper we describe adaptation of a new assay, competitive reverse transcription-PCR (RT-PCR), to monitor botulinum neurotoxin production. This method accurately measures the level of toxin-encoding mRNA inC. botulinum cells. Measurement of mRNA should provide a good indication of gene expression as mRNA is turned over rapidly in bacterial cells. In addition, the method is rapid, specific, and sensitive. The competitive RT-PCR method was developed to examineC. botulinum E VH toxin gene expression and was used to investigate the level of toxin production by C. botulinum E VH when the organism was grown in two different types of broth. The results which we obtained with the competitive RT-PCR method demonstrated that this method is more rapid and more sensitive than the mouse bioassay.
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Van Dyke, M. I., i A. J. McCarthy. "Molecular Biological Detection and Characterization of Clostridium Populations in Municipal Landfill Sites". Applied and Environmental Microbiology 68, nr 4 (kwiecień 2002): 2049–53. http://dx.doi.org/10.1128/aem.68.4.2049-2053.2002.
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ABSTRACT Primer sets specific for 16S rRNA genes were designed for four phylogenetic groups of clostridia known to contain mesophilic cellulolytic species. Specific amplification of these groups from landfill leachate DNA extracts demonstrated the widespread occurrence of clostridia from the Clostridium thermocellum and C. leptum groups. In contrast, the C. botulinum group was never detected, and the C. coccoides-C. lentocellum group was only occasionally detected. Amplification products were analyzed by temporal thermal gel electrophoresis to generate profiles of the clostridial groups and to identify dominant bands. Sequence analysis of 17 landfill clones confirmed that the primers were specific for the clostridial subgroups and that the cloned sequences had a close relationship with known cellulose-degrading clostridia. The primers have therefore been authenticated for use in the rapid identification of clostridia in anaerobic environments.
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Raza, Nadia, Sandhya Dhital, Valerie Elise Espinoza, Roopam Jariwal, Chien-Wai Chiu, Michael Valdez, Arash Heidari, Greti Petersen i Katayoun Sabetian. "Wound Botulism in Black Tar Heroin Injecting Users: A Case Series". Journal of Investigative Medicine High Impact Case Reports 9 (styczeń 2021): 232470962110280. http://dx.doi.org/10.1177/23247096211028078.
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The incidence of wound botulism in injection drug users has increased since the introduction of black tar heroin. Many species of the Clostridium genus, most commonly Clostridium botulinum, Clostridium baratii, and Clostridium butyricum, have been associated with wound botulism. Patients often present with progressive bulbar weakness, including dysphagia, cranial nerve palsies, and loss of speech, in addition to symmetrical descending weakness of the upper extremities that may progress to the chest and lower extremities. In this article, we present 3 cases of wound botulism, in which the patients presented with bulbar weakness and were treated with botulism antitoxin heptavalent. The time to antitoxin administration and its effect on the patients’ clinical courses is compared.
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Ortiz, N. E., i G. R. Smith. "The production of Clostridium botulinum type A, B and D toxin in rotting carcasses". Epidemiology and Infection 113, nr 2 (październik 1994): 335–43. http://dx.doi.org/10.1017/s0950268800051761.
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SUMMARYCarrion is a major source of botulinal toxin for animals. A type D strain of Clostridium botulinum differed from type A and B strains in producing (1) much higher concentrations of toxin in putrefying mouse carcasses at several different temperatures over a period of 35 days, (2) toxicity that sometimes persisted in mouse carcasses for at least a year, and (3) mouse carcasses with exceptionally high oral toxicity. Fish carcasses were much less favourable than mouse carcasses for type D toxigenesis.The study, together with earlier studies on types C and E, indicated that carrion contaminated with C. botulinum type C or D is likely to be particularly dangerous for animals that may ingest it. This accords with the observation that carrion-transmitted botulism in animals is usually caused by type C or D.
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Hanipah, Asti, Kartika Manalu i Rizki Amelia Nasution. "Isolasi dan Identifikasi Bakteri Clostridium Botulinum pada Minyak Jelantah". BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains 6, nr 1 (16.05.2023): 151–59. http://dx.doi.org/10.31539/bioedusains.v6i1.5775.
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The aims of this study were to determine whether Clostridium botulinum bacteria were present in used cooking oil, and to look at the characteristics of Clostridium botulinum bacteria in used cooking oil. In this study, isolation of bacteria from used cooking oil using blood agar media, identification of bacteria using gram staining and biochemical tests such as gelatin test, triple sugar iron agar (TSIA) test, simon citrate test, and motility test were carried out. The results of the study obtained 2 out of 3 samples containing Clostridium botulinum bacteria. These bacteria are more common in used cooking oil, which has a more blackish brown color, lots of crumbs left over from food, and temperatures around 30-55℃. In conclusion, there is Clostridium botulinum bacteria found in used cooking oil. Clostridium botulinum colonies are grey, alpha hemolytic, circular to irregular in shape with scalloped edges or rhizoids, and flat to raised surfaces.
Keywords: Clostridium botulinum, Isolation, Identification, Cooking Oil
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Kuusi, M., V. Hasseltvedt i P. Aavitsland. "Botulism in Norway". Eurosurveillance 4, nr 1 (1.01.1999): 11–12. http://dx.doi.org/10.2807/esm.04.01.00044-en.
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Botulism is a severe neuroparalytic disease caused by toxin produced by Clostridium botulinum, an anaerobic spore-forming bacillus. Physicians in Norway are required to notify the National Institute of Public Health (NIPH) of cases of botulism immediately
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Brett, M. "Botulism in the United Kingdom". Eurosurveillance 4, nr 1 (1.01.1999): 9–11. http://dx.doi.org/10.2807/esm.04.01.00045-en.
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Clostridium botulinum is a spore forming bacterium that grows in the absence of oxygen and is responsible for three main epidemiological categories of disease: foodborne, infant, and wound botulism. Foodborne botulism is an intoxication caused by ingestio
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Gregory, Kyle S., Peter-Rory Hall, Jude Prince Onuh, Otsile O. Mojanaga, Sai Man Liu i K. Ravi Acharya. "Crystal Structure of the Catalytic Domain of a Botulinum Neurotoxin Homologue from Enterococcus faecium: Potential Insights into Substrate Recognition". International Journal of Molecular Sciences 24, nr 16 (12.08.2023): 12721. http://dx.doi.org/10.3390/ijms241612721.
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Clostridium botulinum neurotoxins (BoNTs) are the most potent toxins known, causing the deadly disease botulism. They function through Zn2+-dependent endopeptidase cleavage of SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins, preventing vesicular fusion and subsequent neurotransmitter release from motor neurons. Several serotypes of BoNTs produced by Clostridium botulinum (BoNT/A-/G and/X) have been well-characterised over the years. However, a BoNT-like gene (homologue of BoNT) was recently identified in the non-clostridial species, Enterococcus faecium, which is the leading cause of hospital-acquired multi-drug resistant infections. Here, we report the crystal structure of the catalytic domain of a BoNT homologue from Enterococcus faecium (LC/En) at 2.0 Å resolution. Detailed structural analysis in comparison with the full-length BoNT/En AlphaFold2-predicted structure, LC/A (from BoNT/A), and LC/F (from BoNT/F) revealed putative subsites and exosites (including loops 1–5) involved in recognition of LC/En substrates. LC/En also appears to possess a conserved autoproteolytic cleavage site whose function is yet to be established.
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Souillard, Rozenn, Caroline LE Marechal, Loic Balaine, Sandra Rouxel, Typhaine Poezevara, Valentine Ballan, Marianne Chemaly i Sophie LE Bouquin. "Manure contamination with Clostridium botulinum after avian botulism outbreaks: management and potential risk of dissemination". Veterinary Record 187, nr 6 (25.06.2020): 233. http://dx.doi.org/10.1136/vr.105898.
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BackgroundPersistence of Clostridium botulinum in the environment is well known. Getting rid of it after animal botulism outbreaks is so tricky, especially as far as manure concerns. This study aimed at 1. describing manure management on 10 poultry farms affected by botulism and 2. assessing the persistence of C botulinum in poultry manure after the outbreak.MethodsEach farm was visited twice at two different manure storage times (two weeks after manure removal and two months later). Fifteen samples of manure were collected on each visit and C botulinum was detected using real-time PCR.ResultsManagement of manure varied among poultry farms (classical storage, addition of quicklime, bacterial flora or incineration). C botulinum was detected in the manure of all 10 farms, 56.5per cent of samples being positive. C botulinum was detected significantly more frequently at the second visit (65.8per cent vs 49.7per cent, P<0.01) and on the surface of the pile (63.1per cent vs 50per cent, P=0.025).ConclusionThis study shows the persistence of C botulinum in poultry manure over time after a botulism outbreak and highlights manure management as a key health issue in preventing spore dissemination in the environment and recurrence of the disease.
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MALIZIO, CARL J., JOAN HARROD, KRISTINE M. KAUFMAN i ERIC A. JOHNSON. "Arginine Promotes Toxin Formation in Cheddar Cheese by Clostridium botulinum". Journal of Food Protection 56, nr 9 (1.09.1993): 769–72. http://dx.doi.org/10.4315/0362-028x-56.9.769.
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The production of botulinal toxin by a mixture of spores of Clostridium botulinum types A and B was evaluated in Cheddar cheese supplemented with L-arginine (1% wt/wt) and containing one of three levels of sodium chloride (0, 0.9, or 1.8%). Botulinal toxin was formed in cheeses containing an increased level of L-arginine (1%) and reduced levels of sodium chloride (0 or 0.9%). No toxin was formed in Cheddar with arginine and 1.8% salt or in any of the cheeses not supplemented with arginine. The pH increased from 5.05–5.2 to 5.7–6.0 in the cheeses with increased arginine, but the pH change alone did not permit growth of C. botulinum. Metabolism of arginine may also have promoted the synthesis of compatible metabolites for salt resistance. The results indicate that an important factor supporting growth of C. botulinum in cheese is the availability of L-arginine.
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Glatman-Freedman, Aharona. "Infant Botulism". Pediatrics In Review 17, nr 5 (1.05.1996): 185–86. http://dx.doi.org/10.1542/pir.17.5.185.
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Infant botulism was first described in 1976. It is caused by Clostridium botulinum, a gram-positive anaerobic bacillus found most commonly in soil and agricultural products. The organism forms spores and during growth and germination releases a potent neurotoxin that is responsible for the illness. Eight neurotoxins have been recognized, but infant botulism is caused primarily by organisms producing toxin types A and B. Although adult-type botulism occurs by ingesting food contaminated with botulinus toxin, infant botulism seems to result from ingestion of spores that germinate and release the toxin inside the infant's colon. The toxin is absorbed from the gastrointestinal tract, travels via the blood stream, and binds irreversibly to peripheral cholinergic nerve synapses, where it prevents the release of acetylcholine.
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DERMAN, Y., H. KORKEALA, E. SALO, T. LÖNNQVIST, H. SAXEN i M. LINDSTRÖM. "Infant botulism with prolonged faecal excretion of botulinum neurotoxin and Clostridium botulinum for 7 months". Epidemiology and Infection 142, nr 2 (21.05.2013): 335–39. http://dx.doi.org/10.1017/s0950268813001258.
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SUMMARYIn Finland in April 2010, a 3-month old baby was diagnosed with type A infant botulism. He excreted botulinum neurotoxin and/or Clostridium botulinum in his faeces until November 2010. Five months of excretion was after clinical recovery and discharge from hospital. C. botulinum isolates recovered from the household dust in the patient's home were genetically identical to those found in the infant's stool samples. Long-term faecal excretion of C. botulinum may pose a possible health risk for the parents and others in close contact with the infant.
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SIMUNOVIC, J., J. L. OBLINGER i J. P. ADAMS. "Potential for Growth of Nonproteolytic Types of Clostridium botulinum in Pasteurized Restructured Meat Products: A Review1". Journal of Food Protection 48, nr 3 (1.03.1985): 265–76. http://dx.doi.org/10.4315/0362-028x-48.3.265.
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Type E and nonproteolytic type B strains of Clostridium botulinum can grow and produce toxin at temperatures below 5°C. Recent publications describing the greater heat resistance of nonproteolytic type B C. botulinum spores than type E spores are discussed in relation to suitable proess lethalities required for a safe pasteurized product. The incidences of botulism in Europe caused by nonproteolytic type B spores were compared to the lack of such incidences in the U.S. and to published procedures for isolating the causative agent for botulism. The incidence of C. botulinum spores in meat products in the U.S. also is reviewed.
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Oguma, Keiji, Yukako Fujinaga i Kaoru Inoue. "Clostridium Botulinum Toxin". Journal of Toxicology: Toxin Reviews 16, nr 4 (styczeń 1997): 253–66. http://dx.doi.org/10.3109/15569549709016460.
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Hjerpe, Charles A. "Clostridium botulinum Toxoids". Veterinary Clinics of North America: Food Animal Practice 6, nr 1 (marzec 1990): 229–30. http://dx.doi.org/10.1016/s0749-0720(15)30919-1.
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Hauschild, A. H. W. "Clostridium botulinum toxins". International Journal of Food Microbiology 10, nr 2 (marzec 1990): 113–24. http://dx.doi.org/10.1016/0168-1605(90)90060-i.
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Le Gratiet, Thibault, Typhaine Poezevara, Sandra Rouxel, Emmanuelle Houard, Christelle Mazuet, Marianne Chemaly i Caroline Le Maréchal. "Development of An Innovative and Quick Method for the Isolation of Clostridium botulinum Strains Involved in Avian Botulism Outbreaks". Toxins 12, nr 1 (10.01.2020): 42. http://dx.doi.org/10.3390/toxins12010042.
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Avian botulism is a serious neuroparalytic disease mainly caused by a type C/D botulinum neurotoxin produced by Clostridium botulinum group III, one of the entwined bacterial species from the Clostridium novyi sensu lato genospecies. Its isolation is very challenging due to the absence of selective media and the instability of the phage carrying the gene encoding for the neurotoxin. The present study describes the development of an original method for isolating C. botulinum group III strains. Briefly, this method consists of streaking the InstaGene matrix extraction pellet on Egg Yolk Agar plates and then collecting the colonies with lipase and lecithinase activities. Using this approach, it was possible to isolate 21 C. novyi sensu lato strains from 22 enrichment broths of avian livers, including 14 toxic strains. This method was successfully used to re-isolate type C, D, C/D, and D/C strains from liver samples spiked with five spores per gram. This method is cheap, user-friendly, and reliable. It can be used to quickly isolate toxic strains involved in avian botulism with a 64% success rate and C. novyi sensu lato with a 95% rate. This opens up new perspectives for C. botulinum genomic research, which will shed light on the epidemiology of avian botulism.
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Bowe, Brooke Kathryn, Travis Gwynn Wentz, Brieana Marie Gregg, William Howard Tepp, Kristin Marie Schill, Shashi Sharma i Sabine Pellett. "Genomic Diversity, Competition, and Toxin Production by Group I and II Clostridium botulinum Strains Used in Food Challenge Studies". Microorganisms 10, nr 10 (23.09.2022): 1895. http://dx.doi.org/10.3390/microorganisms10101895.
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Botulinum neurotoxins (BoNTs) produced by the bacteria Clostridium botulinum are the causative agent of human and animal botulism, a rare but serious and potentially deadly intoxication. Foodborne botulism is caused by the consumption of foods containing BoNTs, which results from contamination of foods with C. botulinum spores and toxin production by the bacteria during growth within the food. Validation of the safety of food products is essential in preventing foodborne botulism, however, limited guidance and standards exist for the selection of strains used in C. botulinum food challenge studies. Sequencing and genomics studies have revealed that C. botulinum is a large, diverse, and polyphyletic species, with physiologic and growth characteristics studied only in a few representatives. Little is known about potential growth competition or effects on toxin production between C. botulinum strains. In this study, we investigated an applied cocktail of ten C. botulinum strains, seven Group I and three Group II. Whole genome SNP alignments revealed that this strain cocktail encompasses the major clades of the Group I and II C. botulinum species. While growth competition appears to exist between several of the strains, the cocktail as a whole resulted in high levels of BoNT production.
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de Jong, Laura I. T., Rafael A. Fernández, Virtudes Pareja, Gabriel Giaroli, Sergio R. Guidarelli, Janet K. Dykes i Carolina Lúquez. "First Report of an Infant Botulism Case Due to Clostridium botulinum Type Af". Journal of Clinical Microbiology 53, nr 2 (10.12.2014): 740–42. http://dx.doi.org/10.1128/jcm.02894-14.
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Most infant botulism cases worldwide are due to botulinum toxin types A and B. Rarely,Clostridium botulinumstrains that produce two serotypes (Ab, Ba, and Bf) have also been isolated from infant botulism cases. This is the first reported case of infant botulism due toC. botulinumtype Af worldwide.