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Rozprawy doktorskie na temat „Chromatographic assays”

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Data publikacji: 22 listopada 2023

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1

Serrano, Nadja Fernanda Gonzaga. "Produção de compostos antimicrobianos por Paenibacillus polymyxa RNC-D: otimização das condições de cultivo, purificação e caracterização dos bioprodutos." Universidade Federal de São Carlos, 2014. https://repositorio.ufscar.br/handle/ufscar/273.

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Made available in DSpace on 2016-06-02T19:02:44Z (GMT). No. of bitstreams: 1 6412.pdf: 14036212 bytes, checksum: dc6e90045bca1050bc9fd76f94f1f30a (MD5) Previous issue date: 2014-06-30<br>Financiadora de Estudos e Projetos<br>The increase in the production of antimicrobial metabolites by Paenibacillus polymyxa RNC-D was appraised through the study of cultivation variables. Two process variables, namely the glucose and inoculum concentrations, were evaluated in different levels (5 to 40 g/l, and 2.5% to 5.0% v/v, respectively), and their effects on biomass formation, minimal inhibitory concent

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Zhang, Qunying. "Characterization of receptors for Escherichia coli 987P using competitive binding assays, thin-layer chromatography and gel filtration chromatography." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0023/MQ51825.pdf.

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Embree, Leanne. "Development of a high-performance liquid chromatographic assay for human chorionic gonadotropin as an alternative to the official United States pharmacopeial animal assay." Thesis, University of British Columbia, 1985. http://hdl.handle.net/2429/24637.

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Human chorionic gonadotropin (HCG), a glycoprotein hormone with two nonidentical subunits, is produced by chorionic tissue in pregnant women and by neoplastic tissue containing chorionic elements. It is used in the treatment of male hypogonadism and female sub-fertility. Quantitation of HCG is used to monitor therapy, diagnose various disease states and diagnose and monitor pregnancy. Low levels of HCG in the early and late stages of pregnancy and in various disease states has prompted the development of extremely sensitive assay procedures. Clinically, radioimmunoassay methods are most frequ

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Fillmann, Gilberto. "Appraisal and validation of rapid, integrated chemical and biological assays of environmental quality." Thesis, University of Plymouth, 2001. http://hdl.handle.net/10026.1/2372.

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To assess the significance of pollutants released into the environment it is necessary to determine both the extent of contamination and the biological effects they give rise to. This research is based on a tiered system, which commences with conventional analytical chemistry (gas chromatography), followed by the development, evaluation and application of rapid and simple immunochemical techniques and, finally, the integration of chemical and biological markers to assess pollution. GC-ECD/FID/MS have been used to investigate the status of chemical contamination of the Black Sea by organochlori

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Ahmed, Naila Masud. "Chromatographic assay of advanced glycation endproducts and application to the study of human disease." Thesis, University of Essex, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364507.

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Bhattacharjee, Rathindra Chandra. "Development of a sensitive and stereoselective high performance liquid chromatographic assay method for propafenone enantiomers in human plasma." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/27800.

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Propafenone is a new class 1C antiarrhythmic agent with additional calcium antagonistic and beta-blocking activities. Clinically it is effective in the treatment of supraventricular and ventricular tachycardia, atrial and ventricular fibrillation, ventricular premature contractions and for the management of Wolf-Parkinson-White syndrome. In North America it is still an investigational drug. Propafenone is a chiral drug and is used clinically in the racemic form. The enantiomers of numerous chiral drugs have been shown to differ in their disposition kinetics in the body due to their stereosele

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CHILAKALA, SUJATHA. "DEVELOPMENT OF LIQUID CHROMATOGRAPHY-MASS SPECTROMETRIC ASSAYS AND SAMPLE PREPARATION METHODS FOR THE BIOLOGICAL SAMPLE ANALYSIS." Cleveland State University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=csu1512927043412916.

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Lövgren, Ulf. "Enzyme immunoassay in combination with liquid chromatography for sensitive and selective determination of drugs in biosamples." Lund : Dept. of Analytical Chemistry, Lund University, 1997. http://books.google.com/books?id=Ju1qAAAAMAAJ.

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Kirk, Loren Madden, and Stacy D. Brown. "Beyond-Use Date Determination of Buprenorphine Buccal Solution Using a Stability-Indicating High-Performance Liquid Chromatographic Assay." Digital Commons @ East Tennessee State University, 2015. https://dc.etsu.edu/etsu-works/5305.

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Objectives The objectives of this study included developing and validating a stability-indicating high-performance liquid chromatographic (HPLC) method with ultraviolet (UV) detection for the determination of buprenorphine in a buccal solution for veterinary use, and applying that method to determine the stability of a 3 mg/ml buprenorphine preparation in room temperature and refrigerated storage conditions. This preparation, intended for buccal administration in feline patients, plays an important role in pain management in cats. Methods A stability-indicating HPLC method was developed and va

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Liu, Tina. "Development and validation of an HPLC assay for simethicone in pharmaceutical formulations." Master's thesis, Department of Pharmacy, 2001. http://hdl.handle.net/2123/12307.

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Lovering, A. M. "The development and use of high performance liquid chromatography (HPLC) : Methods in the rapid identification and characterisation of aminoglycoside-acetylating and -phosphorylating enzymes." Thesis, University of the West of England, Bristol, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.371406.

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Gaillard, Isabelle. "Mise en œuvre de la chromatographie liquide haute performance dans le cadre du suivi hors ligne et en ligne des protéines de cultures d'hybridomes : caracterisation de la méthode chromatographique et comparaison avec le test ELISA." Vandoeuvre-les-Nancy, INPL, 1993. http://www.theses.fr/1993INPL022N.

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Depuis la mise au point des techniques de formation des lignées d'hybridomes, des efforts importants sont fournis pour améliorer la productivité des anticorps monoclonaux et pour mieux comprendre le métabolisme cellulaire. Les méthodes analytiques jouent un rôle important dans le contrôle des procédés en permettant la détermination quantitative et qualitative de plusieurs paramètres et variables. L'objectif général de cette étude consiste à contribuer à l'amélioration de la maitrise des bioprocédés en proposant une méthode analytique capable de quantifier les protéines présentes dans les cultu

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Baldassarre, Phyllis Ann. "Comparison of high-performance liquid chromatography (HPLC) and microbiological cylinder plate assays for the determination of erythromycin in uncoated entericoated pellets /." Staten Island, N.Y. : [s.n.], 1990. http://library.wagner.edu/theses/nursing/1990/thesis_nur_1990_balda_compa.pdf.

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Bedard, Pierre R. "Ion interaction liquid chromatography : energetics, mechanism and gradient design considerations for the assay of serum thyroid hormones." Thesis, McGill University, 1985. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=72024.

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The competition between two molecules of similar polarity for adsorption sites on the stationary phase is discussed in light of rapid kinetics of adsorption and desorption, and of the effects of temperature, acetonitrile, surfactant (cyclohexylaminopropane sulfonic acid, CAPS) and salt concentrations on the retention of the thyroid hormones (3,5-diiodothyronine, T2; 3,3',5-triiodothyronine, T3 and thyroxine, T4). A three parameter equation relates the surfactant concentration and ionic strength to the retention of the hormones and is analyzed in terms of the Stern-Gouy-Chapman theory. A second

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Suen, Kin-wah, and 孫建華. "Validation of a HPLC assay for porphobilinogen synthase in human erythrocytes for use in the clinical laboratory." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B29624897.

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(Uncorrected OCR) Abstract Porphobilinogen (PBG) synthase condenses two molecules of aminolaevulinic acid (ALA) to form PBG in heme biosynthesis. The enzyme activity is sensitive to inhibition by heavy metals such as lead. It can act as a biological indicator of chronic lead POis~r\g to identify the risk group, especially in children, so that early treatment can be given to prevent possible permanent damages. A reversed-phase ion-pair HPLC analytical method for the assay of the PBG synthase activity based on detection of PBG production has been validated. A Hypersil CN column (1

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Abi, Aad Elizabeth. "Impact de la Détente Instantanée Contrôlée (DIC) sur l’extraction des molécules bioactives de rhizomes de Rheum ribes L." Electronic Thesis or Diss., La Rochelle, 2023. http://www.theses.fr/2023LAROS008.

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Rheum ribes L. (la rhubarbe) est l'une des plantes médicinales méditerranéennes les plus importantes. La préparation des décoctions aqueuses des rhizomes de cette plante est devenue une pratique courante chez les patients souffrant de diverses maladies. La Détente Instantanée Contrôlée (DIC) est un traitement à haute température et à courte durée (HTST high-temperature/short-time) suivi d'une chute brutale de pression vers le vide (Pression absolue de 5 kPa environ). Le traitement DIC induit l’expansion et réduit la tortuosité de la matrice traitée, et assure ainsi une meilleure diffusivité de

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Holman, Miranda K., Dorcas Frempong, Stacy Brown, Steven Dinh, and Ashana Puri. "Development and Validation of a Liquid Chromatography-Mass Spectrometry Based Analytical Assay for Determination of Cromolyn Sodium in Skin Permeation Studies." Digital Commons @ East Tennessee State University, 2021. https://dc.etsu.edu/asrf/2021/presentations/45.

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Cromolyn sodium (CS) is a mast cell stabilizer which has been used to treat systemic mastocytosis, allergic- and exercise-induced asthma, and allergic reactions induced by atopic dermatitis. Presently, CS is administered orally and intranasally, and with a short half-life and poor absorption, 4 to 8 doses are required daily for treatment. Developing a transdermal product for CS would eliminate such drawbacks that lead to inconsistent patient dosing and provide sustained therapeutic effects when administered via skin. Our long-term goal is to determine the feasibility of delivering CS through s

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Jones, David. "Synthesis, fractionation, characterisation and toxicity of naphthenic acids from complex mixtures." Thesis, University of Plymouth, 2013. http://hdl.handle.net/10026.1/1612.

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Amongst the polar organic compounds occurring in unrefined and refined crude oils and the associated polluted production waters, complex mixtures of acids, known historically as naphthenic acids (NAs), have achieved prominence. This is particularly because NAs have been designated a toxicant class of concern in the oil sands process-affected water (OSPW) that has accumulated in vast quantities following exploitation of the oil sands of Northern Alberta, Canada in recent years. However, though there have been calls for NAs to be added to pollutant inventories, at the initiation of the current s

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Shah, Heta N. "INVESTIGATION OF PHENYLEPHRINE SULFATION AND INHIBITION USING A NOVEL HILIC ASSAY METHOD." VCU Scholars Compass, 2015. http://scholarscompass.vcu.edu/etd/3952.

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Phenylephrine (PE) is the most commonly used over-the-counter nasal decongestant. The problem associated with phenylephrine is that it undergoes extensive first pass metabolism in the intestinal gut wall leading to its poor and variable oral bioavailability. This research project aims at developing strategies in order to increase the oral bioavailability of PE by co-administration of GRAS compounds. A HILIC assay method was developed to detect the parent drug, phenylephrine (PE) and its sulfate metabolite (PES).The enzyme kinetic studies were done with phenolic dietary or GRAS compounds using

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Liu, Xiangli [Verfasser], Alfred Akademischer Betreuer] Fahr, Rolf [Akademischer Betreuer] Schubert, and Gert [Akademischer Betreuer] [Fricker. "High-Throughput Chromatographic Approaches to Assess Drug Partition into Biomembranes / Xiangli Liu. Gutachter: Alfred Fahr ; Rolf Schubert ; Gert Fricker." Jena : Thüringer Universitäts- und Landesbibliothek Jena, 2011. http://d-nb.info/1017078939/34.

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Kovach, Jessica Lynn. "USING ANALYTICAL METHODOLOGIES TO ASSESS THE ORGANOLEPTIC CHARACTER OF CITRUS ESSENTIAL OIL." Diss., Temple University Libraries, 2019. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/590546.

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Chemistry<br>Ph.D.<br>Essential oils are natural products used to flavor food and beverages. With the increase in nutrition conscious consumers, manufacturers of food additives and food products are faced with the challenge of making healthy alternatives. In particular, food products going to market with label claims stating reductions in sugar and salt, organic certified, organic compliant, and all natural; moreover the ingredients used in flavors must meet these label claims as well. More often than not, the challenge in using ingredients that follow these requirements is the pricing, the so

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Harun, Norlida. "Application of molecularly imprinted solid phase extraction, enzyme-linked immunosorbent assay and liquid chromatography tandem mass spectrometry to forensic toxicology." Thesis, University of Glasgow, 2010. http://theses.gla.ac.uk/1992/.

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The rapid growth of ketamine and amphetamine misuse worldwide has led to the development of methods for the detection and analysis of ketamine and amphetamines in biological specimens. Most methods previously developed in forensic toxicology for the detection of ketamine and amphetamines used GC-MS. The present work developed alternative methods based on LC-MS/MS. Ketamine was chosen as the drug of interest because there are no data currently available on the extent of ketamine abuse in Malaysia even though a large amount of ketamine has been seized by the Malaysian Royal Police, while ampheta

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Miller, Eleanor Isabel. "The detection of drugs of abuse in biological matrices using enzyme-linked immunosorbent assay and liquid chromatography-tandem mass spectrometry." Thesis, University of Glasgow, 2007. http://theses.gla.ac.uk/1516/.

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The aim of this study was to investigate the potential use of ELISA and LC-MS-MS in combination and as individual techniques, for the detection of drugs of abuse in biological matrices. Overall the LC-MS-MS method showed good correlation results for opiates compared to the GC-MS method. 6-MAM was however detected in more root segments and segments excluding roots by LC-MS-MS. Morphine was detected in a greater number of root segments by LC-MS-MS compared to GC-MS. However, morphine was detected in a greater number of segments excluding roots by GC-MS. Codeine and dihydrocodeine were also detec

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Klassen, Cherie Leanne. "The development and application of an electron-capture gas chromatographic assay procedure for the simultaneous determination of sertraline and N-desmethylsertraline in biological samples." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ40071.pdf.

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Wang, Ding. "Application of mass spectrometry in enzyme deficiency assay for newborn screening purpose /." Thesis, Connect to this title online; UW restricted, 2006. http://hdl.handle.net/1773/11557.

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Ho, Crystal. "The study of organofluorine analysis applied to totaloxidizable precursor (TOP) assay to understand perandpolyfluoroalkyl substances (PFASs)." Thesis, Örebro universitet, Institutionen för naturvetenskap och teknik, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-69083.

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Per- and polyfluoroalkyl substances (PFASs) are synthetic chemicals which are used in a wide range of applications such as aqueous film forming foam (AFFF) for firefighting, paint, coating and cosmetics. For almost a decade, PFASs have received worldwide attention because of the ubiquitous detection of PFAS in the environment and their bioaccumulative, persistent and give toxic effects on animals and humans. A number of study have shown many unknown organofluorine present in environmental samples. The total oxidizable precursor (TOP) method is an oxidation method to convert any precursor compo

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Summers, Jay W. "Feathers as bioindicators of PCB exposure in clapper rails /." View online, 2009. http://repository.eiu.edu/theses/docs/32211131565121.pdf.

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Embree, Leanne. "Development of a sensitive, quantitative high-performance liquid chromatographic assay for the measurement of digoxin in patient groups with high levels of digoxin-like immunoreactive substances." Thesis, University of British Columbia, 1989. http://hdl.handle.net/2429/29093.

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Digoxin is the most commonly used digitalis glycoside for the treatment of congestive heart failure and certain disturbances of cardiac rhythm. The low therapeutic index observed for digoxin and the clinical significance of digoxin therapy have necessitated the development of sensitive analytical methods for the quantitation of digoxin in biological samples. Digoxin may be analysed by several methods including immunoassays, chromatographic procedures and various biological and chemical methods. Immunoassays, both radioimmunoassay (RIA) and fluorescence polarization immunoassay (FPIA) procedur

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Yalo, Masande Nicholas. "An investigation of the natural products composition of Porphyra capensis (a red seaweed)." University of the Western Cape, 2017. http://hdl.handle.net/11394/6354.

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Magister Scientiae - MSc (Chemistry)<br>Plants have been widely used in traditional medicine for a number of ailments, among which may be included infectious diseases such as colds, influenza, chicken pox, TB, etc. as well as lifestyle diseases such as diabetes and cancer. Seaweeds have also been shown to contribute to the maintenance of health through their nutritional and medicinal properties and recently, a great deal of interest has developed towards the isolation of bioactive compounds from marine sources due to their numerous health benefits. Furthermore, marine algae are valuable source

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Cockcroft, Jennifer Jean. "The development, implementation and validation of a plasma-based high performance liquid chromatographic assay for Isoniazid and N-Acetylisoniazid: an acetylator status population study at Brewelskloof Hospital." Master's thesis, University of Cape Town, 2001. http://hdl.handle.net/11427/26764.

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A novel high performance liquid chromatographic assay has been developed for the simultaneous determination of isoniazid and N-acetylisoniazid in plasma. Solid phase extraction involving C18 columns is used to extract the drug and the metabolite from 0.5 ml plasma. The analyte peaks are resolved using a CB Spherisorb analytical column and ultraviolet detection at 270 nm. The assay is specific to the compounds, with consistent recovery of greater than 75% for isoniazid and over 90% for N-acetylisoniazid. The limits of detection in plasma are 300 ng/ml and 150 ng/ml for isoniazid and N-acetyliso

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El-Khoury, Joe M. "Chronic Kidney Disease: Vitamin D Treatment Regimens and Novel Assay Development for Kidney and Cardiovascular Function Biomarkers." Cleveland State University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=csu1343914060.

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Seno, Hiroshi, Osamu Suzuki, Akira Ishii, et al. "SIMPLE AND RAPID ASSAY METHOD FOR SIMULTANEOUS QUANTIFICATION OF URINARY NICOTINE AND COTININE USING MICRO-EXTRACTION BY PACKED SORBENT AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY." Nagoya University School of Medicine, 2013. http://hdl.handle.net/2237/18475.

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Schirmacher, Anastasiya. "Modification of transmembrane peptides to probe SNARE-induced membrane fusion and cross-presentation of membrane-buried epitopes." Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2020. http://hdl.handle.net/21.11130/00-1735-0000-0005-1576-F.

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GRANDI, F. DE. "DETERMINATION OF LUTEINIZING HORMONE (LH) AND FOLLICLE STIMULATING HORMONE (FSH) IN BOVINE PLASMA: DEVELOPMENT AND VALIDATION OF SPECIES-SPECIFIC MONOCLONAL ANTIBODY-BASED ENZYME-LINKED IMMUNOSORBENT ASSAYS (ELISA)." Doctoral thesis, Università degli Studi di Milano, 2014. http://hdl.handle.net/2434/246436.

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Maximizing livestock reproductive potential is of primary importance in business farming, and it’s the reason for the increasing interest in the development of innovative bio-techniques addressed to the improvement of in vivo and in vitro farm animal fertility. A better knowledge of gonadotropin (i.e.: luteinizing hormone (LH) and follicle stimulating hormone (FSH)) plasma patterns can have a positive impact on animal reproductive efficiency, for example by helping detection of dysfunction of the pituitary-ovarian axis, diagnosis of reproductive disorders, monitoring of antifertility programs

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Moysan-Le, Meur Annie. "Caracterisation de dosage des produits de photoaddition de psoralene dans l'adn "in vitro" et dans l'adn cellulaire." Paris 6, 1987. http://www.theses.fr/1987PA066021.

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Cazedey, Edith Cristina Laignier [UNESP]. "Análise químico-farmacêutica de cloridrato de ciprofloxacino em solução oftálmica." Universidade Estadual Paulista (UNESP), 2009. http://hdl.handle.net/11449/91687.

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Made available in DSpace on 2014-06-11T19:25:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-02-02Bitstream added on 2014-06-13T20:33:02Z : No. of bitstreams: 1 cazedey_ecl_me_arafcf.pdf: 802368 bytes, checksum: 7af4d6526e6d1c491bb231f706185aea (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>O cloridrato de ciprofloxacino, um antibacteriano quinolônico, apresenta amplo espectro de ação e é eficaz in vitro contra praticamente todos os patógenos Gram-negativos, incluindo Pseudomonas aeruginosa. Mostra-se também eficaz contra microrganismos Gram-positiv

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Cazedey, Edith Cristina Laignier. "Análise químico-farmacêutica de cloridrato de ciprofloxacino em solução oftálmica /." Araraquara : [s.n.], 2009. http://hdl.handle.net/11449/91687.

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Orientador: Hérida Regina Nunes Salgado<br>Banca: Magali Benjamin de Araújo<br>Banca: Hérida Regina Nunes Salgado<br>Banca: Greici Cristina Gomes Tozo<br>Resumo: O cloridrato de ciprofloxacino, um antibacteriano quinolônico, apresenta amplo espectro de ação e é eficaz in vitro contra praticamente todos os patógenos Gram-negativos, incluindo Pseudomonas aeruginosa. Mostra-se também eficaz contra microrganismos Gram-positivos, como estafilococos e estreptococos. A importância de desenvolver e validar métodos analíticos para este fármaco é justificada por seu potencial terapêutico, grande emprego

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Duvic, Bernard. "Purification et etude de la proteine de transport de la 20-hydroxyecdysone chez un insecte : locusta migratoria." Université Louis Pasteur (Strasbourg) (1971-2008), 1988. http://www.theses.fr/1988STR13160.

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La purification du transporteur est realisee a partir d'hemolymphe par differentes techniques (coagulation a temperature ambiante; chromatographie sur colonne echangeuse d'anions; chromatographie sur colonne de gel permeation; hplc sur phase echangeuse d'anions). Le transporteur est une glycoproteine acide (pi=5,6) de 280 kda. C'est un homodimere a 2 sous-unites de 140 kda dont la sequence n-terminale a ete determinee. Un polyserum de lapin et 2 anticorps monoclonaux de souris ont ete obtenus et permettent un dosage elisa du transporteur. Des dosages chez l. M. Montrent que le transporteur et

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Eriste, Elo. "Purification, structure and function of bioactive peptides /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7349-853-X/.

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Yamamoto, Célia Hitomi. "Aspectos da qualidade da tetraciclina em preparações farmacêuticas sólidas. Correlação entre os métodos de dosagem por cromatografia líquida de alta eficiência e turbimético." Universidade de São Paulo, 1999. http://www.teses.usp.br/teses/disponiveis/9/9139/tde-05102011-150147/.

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As tetraciclinas são encontradas no mercado sob várias formas farmacêuticas, sendo provenientes de diversos laboratórios farmacêuticos. Com o objetivo de avaliar a qualidade destes medicamentos, foram realizados os ensaios de dissolução in vitro e determinação quantitativa. Um total de 38 amostras de cápsulas de cloridrato de tetraciclina, fosfato de tetraciclina e cloridrato de oxitetraciclina e drágea de cloridrato de doxiciclina, englobando 12 fabricantes, foram analisadas. A dissolução do princípio ativo foi determinada para todas as amostras, conforme o método recomendado pela USP XXIII.

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Ngwalero, Precious. "Development and validation of liquid chromatography mass spectrometry (L/C/MS/MS) assay for the determination of plasma 4betahydroxycholestrol and cholesterol in HIV infected children in Africa." Master's thesis, University of Cape Town, 2015. http://hdl.handle.net/11427/16654.

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Includes bibliographical references<br>4β-hydroxycholesterol (4β-OHC) is a metabolite of cholesterol formed by Cytochrome (CYP) 3A4/5/7 enzymes. It has recently been proposed as an endogenous biomarker forCYP3A4/5/7 activity. This may be useful in prediction of drug-drug interactions and other metabolic processes affected by regulators of CYP3A activity. The aim of this study was to develop and validate an LC/MS/MS assay for the determination of 4β-OHC in human plasma and use 4β-OHC as a biomarker of CYP3A4/5/7 metabolism in HIV-infected children with and without treatment in Africa. Determina

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Ballentine, Regina. "Chemical Characterization of Pseudognaphalium obtusifolium by Gas Chromatography - Mass Spectrometry (GC-MS) to Assess Potential Therapeutic Phytochemicals and Toxicological Concerns Using Simulated Use Conditions." VCU Scholars Compass, 2019. https://scholarscompass.vcu.edu/etd/6052.

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Chemical Characterization of Pseudognaphalium obtusifolium by Gas Chromatography – Mass Spectrometry (GC-MS) to Assess Potential Therapeutic Phytochemicals and Toxicological Concerns Using Simulated Use Conditions By Regina Ballentine Virginia Commonwealth University, 2019 Director: Sarah C. Rutan, Professor, Department of Chemistry Currently, there is an increasing demand for natural therapies and herbal products to treat various ailments. It is generally believed that natural therapies have fewer side-effects than traditional western medicine; however, they are often used in different stre

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Parbhunath, Olivia Leshia. "Validation of two bio-analytical assays for the measurement of hydrophilic antioxidant in several food and beverage commodities in accordance with ISO 17025 regulatory guidelines." Thesis, Cape Peninsula University of Technology, 2013. http://hdl.handle.net/20.500.11838/1495.

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Thesis submitted in fulfilment of the requirements for the degree Master of Technology: Biomedical Technology in the Faculty Health and Wellness Sciences at the Cape Peninsula University of Technology, 2013<br>The accurate and consistent measurement of antioxidants is crucial to evaluating their biological role in the prevention and delay of cancer and other pathological conditions. Hence, the performance of the analytical method utilized should be evaluated for acceptable levels of accuracy, precision and other performance parameters according to internationally accepted standards. Addition

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Kamble, Ujjwala Kerba. "Use of liquid chromatography for assay of flavonoids as key constituents and antibiotics as trace elements in propolis : investigation into the application of a range of liquid chromatography techniques for the analysis of flavonoids and antibiotics in propolis, and extraction studies of flavonoids in propolis." Thesis, University of Bradford, 2016. http://hdl.handle.net/10454/14564.

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Propolis is an approved food additive containing flavonoids as a major active constituent. Variability has been found in the composition of propolis in distinctive regions and it was noticed that there are limitations in the analysis of propolis. In this study, the identification of ten flavonoids and residual antibiotics in propolis was investigated by using several liquid chromatography techniques, including reversed-phase high-performance liquid chromatography (RP-HPLC), microemulsion LC (MELC) and ultra-performance LC (UPLC). The ten flavonoids that were selected for this research include

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Haynes, Christopher Allen. "Development of an assay for fatty acyl-CoAs using liquid chromatography-electrospray ionization-tandem mass spectrometry and its application to the stable isotope labeling and quantitation of sphingolipid metabolism." Diss., Georgia Institute of Technology, 2009. http://hdl.handle.net/1853/37171.

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Fatty acyl-Coenzyme As are metabolites of lipid anabolism and catabolism. A method was developed for their quantitation in extracts of cultured mammalian cells using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). Palmitoyl-CoA (C16:0-CoA) is utilized for de novo sphingolipid biosynthesis catalyzed by serine palmitoyltransferase (SPT), which condenses palmitoyl-CoA and serine to form 3-ketosphinganine. After reduction to form sphinganine (Sa), dihydroceramide synthase (CerS) can N-acylate the Sa using a second fatty acyl-CoA molecule, forming dihydrocera

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Nixon, Christopher E. "2ND TIER ASSAY FOR THE DETECTION OF CONGENITAL ADRENAL HYPERPLASIA BY VIRGINIA’S NEWBORN SCREENING LABORATORY: STEROID PROFILE BY HPLC-MS/MS." VCU Scholars Compass, 2019. https://scholarscompass.vcu.edu/etd/6075.

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Congenital Adrenal Hyperplasia (CAH) encompasses several disorders related to disruptions in the adrenal steroid production pathway. These disruptions may cause virilization of the external female sex organs, incorrect gender assignment, precocious puberty, and in the most severe form, may cause life-threatening salt wasting and adrenal crisis if not detected and treated early in the newborn period. 17α-Hydroxyprogesterone (17-OHP) is the primary target for immunofluorescence detection of CAH from dried blood spots in newborn screening (NBS). Unfortunately, current immunoassay techniques for t

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Menou, Sophie. "Comparaison de méthodes d'analyses de traces de pesticides organiques dans les productions végétales, par chromatographies et immunodosages (ELISA)." Angers, 1995. http://www.theses.fr/1995ANGE0005.

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Les travaux consignés dans ce mémoire avaient pour objectif de situer les méthodes immunoenzymatiques ELISA dans l'ensemble des procédés d'analyse de résidus de pesticides dans les végétaux : limite de détection, sensibilité, étendue du domaine d'utilisation, sélectivité, reproductibilité et répétabilité, précision et dispersion des résultats, commodité d'emploi, perspectives de perfectionnement dans une première partie, on a rappelé quelques généralités relatives aux résidus de produits phytosanitaires et à leur dosage par les techniques chromatographiques conventionnelles. Dans une deuxième

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Letourneur, Didier. "Polymères fonctionnels phosphorylés : interactions biospécifiques avec des protéines humaines, anticorps anti-adn, antiphospholipides et facteurs de transcription." Paris 13, 1988. http://www.theses.fr/1988PA132010.

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Des résines dérivées du polystyrene réticule ont été synthétisées. Ces polymères sont fonctionnalisés par des groupements chimiques analogues à ceux de l'adn et des phospholipides. L'affinité biospécifique de ces resines pour des protèines humaines qui admettent pour substrat l'adn et les phospholipides : anticorps anti adn, anti phospholipide, facteurs de transcription de l'adn. Les anticorps qui sont présents dans le sang des patients atteints de lupus érythemateux dissemine (l. E. D) ont montré une affinité spécifique tres élevée pour les dérivés fonctionnels du polystyrene;ces interactions

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Zech, Julie [Verfasser], Leif-Alexander [Akademischer Betreuer] Garbe, Hajo [Gutachter] Haase, Juri [Gutachter] Rappsilber, and Sascha [Gutachter] Rohn. "Analysis of bisphenols and bisphenol A diglycidyl ethers by stable isotope dilution assay liquid chromatography-tandem mass spectrometry / Julie Zech ; Gutachter: Hajo Haase, Juri Rappsilber, Sascha Rohn ; Betreuer: Leif-Alexander Garbe." Berlin : Technische Universität Berlin, 2016. http://d-nb.info/1156011728/34.

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Magnér, Jörgen. "Methodologies to assess the fate of polar organic compounds in aquatic environments." Doctoral thesis, Stockholms universitet, Institutionen för tillämpad miljövetenskap (ITM), 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-35552.

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Polar organic compounds (POCs) are chemicals with polar functional groups in their structure. The functional groups make the compounds hydrophilic and less prone to partition with biota. However, the knowledge of their fate is limited due to difficulties associated with their measurements. Although, the persistence of POCs in the environment is generally low, they are considered to be semi-persistent compounds due to their continuous introduction to the environment via wastewater. Studies have shown that complex mixtures of POCs of different classes may have synergistic toxic effects on biota

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