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Artykuły w czasopismach na temat "Chick heart"

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Boehm, C., T. R. Johnson, J. D. Caston i R. J. Przybylski. "Cardiac hypertrophy in chick embryos induced by hypothermia". American Journal of Physiology-Cell Physiology 252, nr 1 (1.01.1987): C97—C104. http://dx.doi.org/10.1152/ajpcell.1987.252.1.c97.

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A decrease in incubation temperature from 38 to 32 degrees C elicits a decrease in chicken embyro size and weight with concomitant heart enlargement if done after day 10 of incubation. When assayed at day 18 of incubation with the hypothermia started on day 11 or 14, evidence is presented that the heart enlargement is an hypertrophy with no detectable hyperplasia. Supporting data are presented for various physical parameters showing increases in heart wet and dry weight, volume, area, wall thickness, and cell size. There was little difference in DNA content and nuclear [3H]thymidine labeling index between hearts of control and hypothermic embryos. Hearts of hypothermic embryos showed a slight increase in water content and considerable increases in RNA, protein, and glycogen content per unit DNA. The average size of polysomes isolated from hypothermic hearts was larger than that of polysomes isolated from controls. Microscopic studies showed no obvious increase in amount of capillary beds, connective tissue, and myocardial cells. Annulate lamellae were found only in myocardial cells of hypothermic embryos in sparse amounts and low frequency but always associated with large deposits of glycogen.
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Anderson, R. H. "The developing heart in chick embryos." Circulation 82, nr 4 (październik 1990): 1542–43. http://dx.doi.org/10.1161/circ.82.4.2401086.

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Janikovičová, L., Z. Demčišáková, L. Luptáková i Petrovová E. "Pre-Incubation and its Effect on the Development and Malformations of The Chick Embryo". Folia Veterinaria 63, nr 1 (1.03.2019): 24–31. http://dx.doi.org/10.2478/fv-2019-0004.

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Abstract This study was conducted to evaluate the effect of eggs stored with and without pre-incubation on chick embryos with emphasis on: embryo body, heart weight, malformations, and mortality. For this study, a total of 120 chick embryos were divided into three groups, based on the length of storage before hatching (3, 7 and 10 days). Observations of the weight of chick embryo bodies, chick embryo hearts, and the level of mortality and appearance of malformations were noted. With an increase in days stored, the chick embryo’s weight decreased. The pre-incubation period had a positive effect on the weight of chick embryo, and chick hearts. Malformations, including: hydrocephalus, open body cavity and underdeveloped wings, were observed in all three groups, with the highest proportion seen in the pre-incubated hatching eggs stored for 10 days; this group also displayed the highest level of mortality. Non-pre-incubated eggs showed the most promise with better results in all experimental groups. In conclusion, the research suggests the optimal storage for chick embryos to be 3 days, with lowest levels of mortality, malformations and limited effects on the body and heart weight.
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Hoyle, C., N. A. Brown i L. Wolpert. "Development of left/right handedness in the chick heart". Development 115, nr 4 (1.08.1992): 1071–78. http://dx.doi.org/10.1242/dev.115.4.1071.

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The chick heart tube develops from the fusion of the right and left areas of precardiac mesoderm and in almost all cases loops to the embryo's right-hand side. We have investigated whether any intrinsic difference exists in the right and left areas of precardiac mesoderm, that influences the direction of looping of the heart tube. Chick embryos incubated to stages 4,5 and 6 were cultured by the New method. Areas of precardiac mesoderm were exchanged between donor and host embryos of the same stage and different stages to form control, double-right and double-left sided embryos. Overall, double-right sided embryos formed many more left-hand loops than double-left sided embryos. At stages 4 and 5 a small percentage of double-right embryos formed left-hand loops (13%) whereas at stage 6 almost 50% of hearts had left-hand loops. Control embryos formed right-hand loops in 97% of cases. The stability of right-hand heart looping by double-left sided embryos, may be related to the process of ‘conversion’, whereas the direction of looping by double-right sided embryos has become randomised. There is some indication that an intrinsic change occurred in the precardiac mesoderm between stages 5 and 6 that later influenced the direction of looping of the heart tube. The direction of body turning is suggested to be linked to the direction of heart looping.
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Bkaily, G., A. Sculptoreanu, D. Jacques, D. Economos i D. Menard. "Apamin, a highly potent fetal L-type Ca2+ current blocker in single heart cells". American Journal of Physiology-Heart and Circulatory Physiology 262, nr 2 (1.02.1992): H463—H471. http://dx.doi.org/10.1152/ajpheart.1992.262.2.h463.

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Apamin, a bee venom polypeptide, was reported to block the naturally occurring Ca2+ slow action potentials (APs) in cultured cell reaggregates from old chick hearts [Bkaily, G. et al. Am. J. Physiol. 248 (Heart Circ. Physiol. 17): H961-H965, 1985] as well as the tetrodotoxin (TTX)- and Mn(2+)-insensitive slow Na+ current in young embryonic chick heart cells (Bkaily, G. In Vitro Toxicology. Academic, In press; Bkaily et al. J. Mol. Cell. Cardiol. 23: 25-39, 1991). With the use of the whole cell voltage-clamp technique in single ventricular cells from 10-day-old chick embryos and 17- to 20-wk-old human fetuses, two types of Ca2+ currents (ICa), T and L, were found. These two types of slow inward current in both heart preparations were nearly similar in their voltage, kinetics, and pharmacology. Apamin, a slow Ca2+ action potential blocker in old embryonic chick heart, was found to block the L-type ICa (IL) in a dose-dependent manner without affecting the T-type ICa in both heart cell preparations. The blockade of the IL by apamin was completely reversible upon washout with apamin-free solution. Therefore, when compared with nifedipine or to PN 200-110, apamin seems to be a highly potent L-type Ca2+ channel blocker in heart cells.
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Barnett, J. V., M. Taniuchi, M. B. Yang i J. B. Galper. "Co-culture of embryonic chick heart cells and ciliary ganglia induces parasympathetic responsiveness in embryonic chick heart cells". Biochemical Journal 292, nr 2 (1.06.1993): 395–99. http://dx.doi.org/10.1042/bj2920395.

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We have developed a system for the co-culture of embryonic chick heart cells obtained from embryos at 3.5 days in ovo with ciliary ganglia from chick embryos at 7 days in vivo. After 3 days of co-culture, removal of the ciliary ganglia resulted in complete degeneration of axons within 6-8 h, leaving the post-innervated heart cell culture devoid of neurons. Embryonic chick heart cells at 3.5 days in ovo are unresponsive to muscarinic stimulation. However, following 3 days of co-culture with ciliary ganglia, the heart cells developed a negative chronotropic response to muscarinic stimulation (paired t test, P < 0.02) which persisted for at least 24 h after removal of the ciliary ganglion. The development of muscarinic responsiveness was associated with an increase in the levels of specific alpha-subunits of the guanine nucleotide binding proteins (G-proteins), with a 3-fold increase in the level of alpha 39 (39 kDa subunit) and a 2.5-fold increase in the level of alpha 41. The level of the G-protein subunit alpha s remained unchanged. Culture of embryonic chick heart cells at 3.5 days in ovo with medium conditioned by the growth of embryonic chick heart cells and ciliary ganglia had an effect on the chronotropic response to muscarinic stimulation and on alpha 39 and alpha 41 levels identical to that of co-culture. These data suggest that a soluble factor released during the co-culture of embryonic chick heart cells and ciliary ganglia is capable of inducing muscarinic responsiveness. These studies suggest that innervation of the heart may induce parasympathetic responsiveness by increasing the availability of G-proteins which couple the muscarinic receptor to a physiological response.
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Raddatz, E., M. Servin i P. Kucera. "Oxygen uptake during early cardiogenesis of the chick". American Journal of Physiology-Heart and Circulatory Physiology 262, nr 4 (1.04.1992): H1224—H1230. http://dx.doi.org/10.1152/ajpheart.1992.262.4.h1224.

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Oxidative metabolism of the isolated embryonic heart of the chick has been determined using a spectrophotometric technique allowing global as well as localized micromeasurements of the O2 uptake. Entire hearts, excised from embryos of 10 somites (primordia fused, stage 10 HH) and 40 somites (S shaped, stage 20 HH) were placed in a special chamber under controlled metabolic conditions where they continued to beat spontaneously and regularly. During the 32 h of development, the O2 consumption of the whole heart increased from 0.9 +/- 0.1 to 5.3 +/- 0.8 nmol O2/h. These values corrected for protein content were, however, comparable (0.45 nmol O2.h-1.micrograms-1). At stage 10-12, the O2 uptake varied along the cardiac tube (from 0.74 to 1.0 nmol O2.h-1.mm-2). From stage 10 to 20, the O2 uptake per unit area of ventricle wall increased from 0.7 +/- 0.2 to 1.8 +/- 0.2 nmol O2.h-1.mm-2, and the O2 uptake per myocardial volume during one cardiac cycle varied from 7 to 2.5 nmol O2/cm3. These results indicate that, despite an intense morphogenesis, the cardiac tissue has a rather low and stable oxidative metabolism, although the O2 requirement of the whole heart increases significantly. Moreover, the normalized suprabasal aerobic energy expenditure decreases throughout early cardiogenesis. The functional integrity of the isolated embryonic heart combined with the experimental possibilities of the microtechnique make the preparation appropriate for studying the changes in cardiac metabolism during development.
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Taber, Larry A., i Renato Perucchio. "Stress-Strain Relations in Embryonic Chick Heart". American Journal of Physiology-Heart and Circulatory Physiology 281, nr 1 (1.07.2001): H463—H466. http://dx.doi.org/10.1152/ajpheart.2001.281.1.h463.

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STONE, BRADLEY A., MELVYN LIEBERMAN i WANDA KRASSOWSKA. "Field Stimulation of Isolated Chick Heart Cells." Journal of Cardiovascular Electrophysiology 10, nr 1 (styczeń 1999): 92–107. http://dx.doi.org/10.1111/j.1540-8167.1999.tb00646.x.

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Kagawa, K., i H. Kagawa. "DNA modification in chick heart and cerebrum". Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 138, nr 2 (czerwiec 2004): 147–60. http://dx.doi.org/10.1016/j.cbpb.2004.03.001.

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Rozprawy doktorskie na temat "Chick heart"

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Brochu, Richard. "Pacemaking in embryonic chick heart". Thesis, McGill University, 1990. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=59533.

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Experiments were conducted to determine the currents involved in the pacemaker activity of aggregates and single cells from the embryonic chick heart. Two microelectrode voltage clamp studies of embryonic chick ventricular heart cell aggregates revealed two time-dependent current components in the pacemaker range of potentials ($-$60 to $-$120 mV). Barium (Ba, 5 mM) blocked the more negatively activated time-dependent component unmasking a component which remained inwardly directed for hyperpolarizing steps beyond the potassium equilibrium potential (E$ sb{ rm K}$). This component, which was blocked by cesium (Cs, 2 mM), is consistent with an inward current which activates upon hyperpolarization (the I, model) as proposed by DiFrancesco (1981a,b), for Purkinje fibers.
In order to minimize the problems associated with the accumulation/depletion of ions in the extracellular space during voltage clamp experiments, studies were carried out on single ventricular cells or small clusters of ventricular cells.
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Sneesby, Kyra, i n/a. "Gene Expression in Embryonic Chick Heart Development". Griffith University. School of Biomolecular and Biomedical Science, 2003. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20030924.153514.

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Establishment of the biochemical and molecular nature of cardiac development is essential for us to understand the relationship between genetic and morphological aspects of heart formation. The molecular mechanisms that underly heart development are still not clearly defined. To address this issue we have used two approaches to identify genes involved in early chick cardiac development. Differential display previously conducted in our laboratory led to the identification of two gene fragments differentially expressed in the heart that are further described in this thesis. The full-length cDNA sequence of both eukaryotic translation initiation factor-2b (eIF-2b) and NADH cytochrome b5 reductase (b5R) were isolated using library screening. The upreglation of these genes during heart development is expected given the heart is the first functional organ to form in vertebrates and protein synthesis and cell metabolism at this stage of development is maximal. Limitations in the differential display approach led to the development and optimisation of a subtractive hybridisation approach for use with small amounts of cells or tissue. To focus on cardiac gene expression during the initial phases of heart development, subtractive hybridization was performed between the cardiogenic lateral plate mesoderm of Hamburger and Hamilton stage 4 embryos and the heart primordia of stage 9 embryos. Of the 87 independent clones identified by this procedure, 59 matched known sequences with high homology, 25 matched unknown expressed sequence tag (EST) sequences with high homology, and 3 did not match any known sequence on the database. Known genes isolated included those involved in transcription, translation, cell signalling, RNA processing, and energy production. Two of these genes, high mobility group phosphoprotein A2 (HMGA2) and C1-20C, an unknown gene, were chosen for further characterisation. The role of each gene in early chick heart development and indeed development in general, was addressed using techniques such as in situ hybridisation, transfection analysis, in ovo electroporation and RNAi. HMGA2 is a nuclear phosphoprotein commonly referred to as an architectural transcription factor due to its ability to modulate DNA conformation. In keeping with this function, HMGA2/GFP fusion protein was shown to localise to the nucleus and in particular, the nucleolus. In situ hybridisation analysis suggested a role for HMGA2 in heart and somite development. HMGA2 expression was first detected at HH stage 5 in the lateral plate mesoderm, a region synonymous with cells specified to the cardiac fate. HMGA2 was also strongly expressed in the presomitic segmental plate mesoderm and as somites developed from the segmental plate mesoderm, the expression of HMGA2 showed an increasingly more restricted domain corresponding to the level of maturation of the somite. Restriction of HMGA2 expression was first detected in the dorsal region of the epithelial somite, then the dorsomedial lip of the dermomyotome, and finally the migrating epaxial myotome cells. The novel intronless gene, C1-20C, predicts a protein of 148 amino acids containing a putative zinc finger binding domain and prenyl binding motif. Zinc binding assays showed that the zinc finger domain of C1-20C/MBP fusion protein bound over six times the quantity of zinc compared to MBP alone, although not in a 1:1 stoichiometric molar ratio. C1-20C/GFP fusion protein was shown to localise to as yet unidentified intracellular cytoplasmic vesicular compartments. These compartments did not colocalise with the endosome/lysosome pathway, aparently ruling out a role for C1-20C in protein trafficking, recycling or degradation. Expression of C1-20C in the chick embryo suggests a possible role in heart and notochord development and preliminary results using siRNA suggest that C1-20C is involved in normal heart looping.
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Sneesby, Kyra. "Gene Expression in Embryonic Chick Heart Development". Thesis, Griffith University, 2003. http://hdl.handle.net/10072/367647.

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Establishment of the biochemical and molecular nature of cardiac development is essential for us to understand the relationship between genetic and morphological aspects of heart formation. The molecular mechanisms that underly heart development are still not clearly defined. To address this issue we have used two approaches to identify genes involved in early chick cardiac development. Differential display previously conducted in our laboratory led to the identification of two gene fragments differentially expressed in the heart that are further described in this thesis. The full-length cDNA sequence of both eukaryotic translation initiation factor-2b (eIF-2b) and NADH cytochrome b5 reductase (b5R) were isolated using library screening. The upreglation of these genes during heart development is expected given the heart is the first functional organ to form in vertebrates and protein synthesis and cell metabolism at this stage of development is maximal. Limitations in the differential display approach led to the development and optimisation of a subtractive hybridisation approach for use with small amounts of cells or tissue. To focus on cardiac gene expression during the initial phases of heart development, subtractive hybridization was performed between the cardiogenic lateral plate mesoderm of Hamburger and Hamilton stage 4 embryos and the heart primordia of stage 9 embryos. Of the 87 independent clones identified by this procedure, 59 matched known sequences with high homology, 25 matched unknown expressed sequence tag (EST) sequences with high homology, and 3 did not match any known sequence on the database. Known genes isolated included those involved in transcription, translation, cell signalling, RNA processing, and energy production. Two of these genes, high mobility group phosphoprotein A2 (HMGA2) and C1-20C, an unknown gene, were chosen for further characterisation. The role of each gene in early chick heart development and indeed development in general, was addressed using techniques such as in situ hybridisation, transfection analysis, in ovo electroporation and RNAi. HMGA2 is a nuclear phosphoprotein commonly referred to as an architectural transcription factor due to its ability to modulate DNA conformation. In keeping with this function, HMGA2/GFP fusion protein was shown to localise to the nucleus and in particular, the nucleolus. In situ hybridisation analysis suggested a role for HMGA2 in heart and somite development. HMGA2 expression was first detected at HH stage 5 in the lateral plate mesoderm, a region synonymous with cells specified to the cardiac fate. HMGA2 was also strongly expressed in the presomitic segmental plate mesoderm and as somites developed from the segmental plate mesoderm, the expression of HMGA2 showed an increasingly more restricted domain corresponding to the level of maturation of the somite. Restriction of HMGA2 expression was first detected in the dorsal region of the epithelial somite, then the dorsomedial lip of the dermomyotome, and finally the migrating epaxial myotome cells. The novel intronless gene, C1-20C, predicts a protein of 148 amino acids containing a putative zinc finger binding domain and prenyl binding motif. Zinc binding assays showed that the zinc finger domain of C1-20C/MBP fusion protein bound over six times the quantity of zinc compared to MBP alone, although not in a 1:1 stoichiometric molar ratio. C1-20C/GFP fusion protein was shown to localise to as yet unidentified intracellular cytoplasmic vesicular compartments. These compartments did not colocalise with the endosome/lysosome pathway, aparently ruling out a role for C1-20C in protein trafficking, recycling or degradation. Expression of C1-20C in the chick embryo suggests a possible role in heart and notochord development and preliminary results using siRNA suggest that C1-20C is involved in normal heart looping.
Thesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Biomolecular and Biomedical Sciences
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Potter, M. J. "Heart rate and behaviour in the domestic chick". Thesis, University of Reading, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376787.

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Warner, Louise. "Morpho-mediated alpha myosin heavy chain knockdown in the developing chick heart". Thesis, University of Nottingham, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.486713.

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McKinnon, Lise Anne. "Developmental regulation of muscarinic acetylcholine receptor expression in embryonic chick heart and retina /". Thesis, Connect to this title online; UW restricted, 1997. http://hdl.handle.net/1773/6263.

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Waddell, Trinity Q. "Role of Transient Receptor Potential Channels in Epithelial Morphogenesis in Chick Embryo". BYU ScholarsArchive, 2019. https://scholarsarchive.byu.edu/etd/8112.

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Transient Receptor Potential channels (TRP) are a superfamily of cationic specific ionchannels that are regulated by various stimuli such as temperature, pH, mechanical stress, ligandsand ion concentration. The role of TRP channels in disease states such as autosomal dominantpolycystic kidney disease, cancer metastasis, and developmental defects lend credence to thebelief that they play an important part in epithelial morphogenesis events. The development ofsomites, neural tube closure and migration of neural crest cells to form things such as the faceand heart is a good developmental model for the aforementioned cellular processes. We haveshown that TRP channels can be found in the developing ectoderm, hindbrain, and heart and thatthe inhibition of TRP channels in a developing embryo results in phenotypes suggestingperturbation of cellular remodeling processes. This leads to the question of the specific role ofTRP channels in the epithelial mesenchymal transition and remodeling in developing chickembryos.
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Ahir, Bhaveshkumar. "A Study of Developmental Toxicity using Chick Heart Micromass and D3 Mouse Embryonic Stem Cells - A Possible Role for Connexins". Thesis, University of Nottingham, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.517796.

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Nilsson, Angelica. "Optimisation of a method for isolation of Clostridium difficile from faeces". Thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-126892.

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Clostridium difficile is a pathogen for both humans and animals and is often associated with antibiotic-associated diarrhea. Recently, several human cases of C. difficile-infection with increased mortality and morbidity have been reported. In studies performed in different countries C. difficile has been found in meat. Therefore the question whether C. difficile can be a zoonotic agent has been raised. The aim of this study was to optimize a method for isolation of C. difficile from faeces. When C. difficile is isolated from animals that do not have diarrhea the sample must be cultivated in an enrichment broth. Parameters influencing the enrichment were tested such as enrichment before and after spore selection, enrichment time, alcohol and heat chock for spore selection and if the samples had to be centrifuged or not before cultivation on agar plates. Enrichment in broth before spore selection was better than after. Heat and alcohol chock showed similar results, therefore you can chose which method you want. Cultivation from the pellet after centrifugation of the sample was better than cultivating directly from the inoculated broth. When the sample had low concentration of bacteria long enrichment time, 7 days or more, was best. The next step will be isolation of C. difficile from food-producing animals and humans and the strains will then be compared to se if the same strain is found in humans and in animals, to se if C. difficile-infection can be a zoonoz.
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Özkan, Şule Aydın Serpil. "SDÜ aile hekimliği ve check-up polikliniğine başvuran olgularda kardiyovaskuler risk faktörlerinin araştırılması /". Isparta: SDÜ Tıp Fakültesi, 2006. http://tez.sdu.edu.tr/Tezler/TT00256.pdf.

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Książki na temat "Chick heart"

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Brown, Denise Anne. Microtubule subpopulations in chick heart fibroblasts. Norwich: University of East Anglia, 1992.

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How to keep your cholesterol in check. Wyd. 3. London: Sheldon Press, 2006.

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US GOVERNMENT. An Act to Designate the Facility of the United States Postal Service Located at 5805 White Oak Avenue in Encino, California, as the "Francis Dayle 'Chick' Hearn Post Office.". [Washington, D.C: U.S. G.P.O., 2002.

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Senanayake, Sanjaya. Chilli, chicks & heart-attacks: The misadventures of an intern : by Dr. Manjula Mendis. Colombo: Bay Owl Press, 2010.

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Senanayake, Sanjaya. Chilli, chicks & heart-attacks: The misadventures of an intern : by Dr. Manjula Mendis. Colombo: Bay Owl Press, 2010.

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Wieners, Brad. Reality check: You've heard the hype ; wired asked the experts ; here's the real future. San Francisco: HardWired, 1996.

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Anthony, Dr A'Shellarien. The Chick On The Side: From the Heart of a Wife. CreateSpace Independent Publishing Platform, 2012.

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Blair, T. Ann. Ontogeny of A₁ adenosine receptor-mediated negative chronotropy in embryonic chick heart. 1990.

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Activity, Nell. Easter Coloring Activity and Coloring Book: 35 Coloring for Men Wreath, Easter Egg, Easter Bunny, Cross, Chicken, Heart, Egg, Chick Image Quizzes Words and Coloring Book. Independently Published, 2020.

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Activity, Val. Easter Egg Coloring Book: Activity and Coloring Book 50 Activity Icon, Willow, Sign, Rose, Chick, Easter Egg, Wreath, Heart for Boys Ages 4-8 Picture Quiz Words. Independently Published, 2020.

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Części książek na temat "Chick heart"

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Bracke, Marc E., Virinder S. Parmar, Anthony L. DePass, Christian V. Stevens, Barbara W. Vanhoecke i Marc M. Mareel. "Chick Heart Invasion Assay". W Methods in Molecular Biology, 93–106. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4614-8244-4_7.

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Nag, A. C. "Embryonic Chick Heart Muscle Cells". W Cell Culture Techniques in Heart and Vessel Research, 3–19. Berlin, Heidelberg: Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-75262-9_1.

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Sperelakis, N., T. Yamamoto, G. Bkaily, H. Sada, A. Sawamura i J. Azuma. "Taurine Effects on Action Potentials and Ionic Currents in Chick Myocardial Cells". W Taurine and the Heart, 1–19. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4613-1647-3_1.

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Lobaugh, L. Anderson, S. Liu i M. Lieberman. "NA/K Pump Function in Cultured Embryonic Chick Heart Cells". W Developments in Cardiovascular Medicine, 181–90. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4613-2053-1_11.

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Toth, Peter P., Britton Chance, John E. Sell, John F. Holland, Shelagh Ferguson-Miller i Clarence H. Suelter. "The Kinetics of Inorganic Phosphate Uptake and Utilization by Chick Heart Mitochondria". W Integration of Mitochondrial Function, 313–23. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4899-2551-0_28.

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Midgett, Madeline, i Sandra Rugonyi. "Analysis of 4D Myocardial Wall Motion During Early Stages of Chick Heart Development". W Methods in Molecular Biology, 191–212. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2572-8_16.

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Osmond, Mark. "The Effects of Retinoic Acid on Early Heart Formation and Segmentation in the Chick Embryo". W Formation and Differentiation of Early Embryonic Mesoderm, 275–300. Boston, MA: Springer US, 1992. http://dx.doi.org/10.1007/978-1-4615-3458-7_23.

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Nishikawa, T., E. F. Gilbert i H. J. Bruyere. "Potentiating Effects of Caffeine on Ephedrine-Induced Conotruncal Malformations of the Embryonic Heart in the Chick". W Pediatric Cardiology, 908–10. New York, NY: Springer New York, 1986. http://dx.doi.org/10.1007/978-1-4613-8598-1_245.

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Yamagishi, Toshiyuki, Yuji Nakajima, Katsumi Ando i Hiroaki Nakamura. "Msx1 Expression During Chick Heart Development: Possible Role in Endothelial-mesenchymal Transformation During Cushion Tissue Formation". W Cardiovascular Development and Congenital Malformations, 69–71. Malden, Massachusetts, USA: Blackwell Publishing Ltd, 2007. http://dx.doi.org/10.1002/9780470988664.ch17.

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Brown, J. H., i S. B. Masters. "Differential Effects of Carbachol and Oxotremorine on Muscarinic Receptors, Cyclic Amp Formation, and Phosphoinositide Turnover in Chick Heart Cells". W Advances in Behavioral Biology, 939–46. Boston, MA: Springer US, 1986. http://dx.doi.org/10.1007/978-1-4684-5194-8_91.

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Streszczenia konferencji na temat "Chick heart"

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Choma, Michael A., T. Yelbuz, Lars Thrane, Margaret L. Kirby i Joseph A. Izatt. "Three-dimensional OCT imaging of the embryonic chick heart". W Biomedical Optics 2003, redaktorzy Valery V. Tuchin, Joseph A. Izatt i James G. Fujimoto. SPIE, 2003. http://dx.doi.org/10.1117/12.479042.

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Moriya, Kenji, Yuya Chiba i Yoshiko Maruyama. "Heart Rate Responses to Acoustic Stimuli in Prenatal Chick Embryos". W International Conference on Industrial Application Engineering 2018. The Institute of Industrial Application Engineers, 2018. http://dx.doi.org/10.12792/iciae2018.007.

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Yao, Jiang, Victor D. Varner, Renato Perucchio i Larry A. Taber. "Viscoelastic Material Properties of the Hamburger-Hamilton Stage 12 Chick Heart". W ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-192766.

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Mechanical force is believed to play a significant role in regulating the morphogenetic process of cardiac looping. To better understand this process, it is crucial to determine the material properties of the early chick heart. It is well known that biological tissues are viscoelastic, however previous data on early stage embryonic heart tissue shows a hyperelastic behavior only [1] and currently, only late stage heart tissues have been quantified using viscoelastic properties [2]. The objective of this study is to use microindentation and nonlinear finite element method (FEM) to characterize the viscoelastic material properties of stage 12 chick heart during cardiac looping.
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Alser, Maha W., Huseyin Enes Salman i Huseyin Cagatay Yalcin. "Altered Inflow Hemodynamics affects Heart Development in a Side Specific Manner in the Embryonic Heart". W Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0172.

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Background: Hemodynamics, forces from the flowing blood in the heart, is a major epigenetic factor for heart development. Disturbed hemodynamics were shown to induce cardiac malformations in the embryonic heart. Clinically relevant congenital heart defects (CHDs) can be introduced surgically in the lab by disturbing the hemodynamics, like Hypoplastic left heart syndrome (HLHS), characterized by underdeveloped left ventricle is underdeveloped. Left atrial ligation (LAL) on chick embryo is an experimental technique to produce a HLHS-like phenotype. Aims: To reveal mechanobiological mechanisms associated with disturbed hemodynamics that influence the progression of left ventricular hypoplasia using chick embryo model. We also introduce a new technique which we called right atrial ligation (RAL), to examine effect of flow disturbance in right heart. Methods: We combined multiple novel techniques in this research: Heart function was assessed via Echocardiography. Computational fluid dynamics (CFD) analysis was adapted for detailed hemodynamics assessment, such as wall shear stress and blood flow patterns. Heart morphology was assessed by histology, and micro-CT. Results: Echocardiography and CFD analysis showed flow and WSS levels decreased for the flow constricted side resulting in the flow diversion to the opposite side: LAL diverted flow to right side and RAL to left side. This disturbance resulted in underdevelopment of left heart (valve and ventricle) in LAL and underdevelopment of right heart in RAL, revealed with histology and micro-CT. Left side was affected more compared to right side, demonstrating higher plasticity in left heart. Conclusion: This study indicates the critical importance of altered inflow hemodynamics in cardiac development specifically valve and ventricle development. Our comprehensive approach can be used to predict the initiation and growth of congenital heart defects.
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Periasamy, A., J. R. Lindroth i R. P. Thompson. "Real time analysis of video image and electrocardiogram of the chick embryo heart". W Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 1988. http://dx.doi.org/10.1109/iembs.1988.94620.

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Filas, Benjamen A., i Larry A. Taber. "Surface Strains in the Looping Embryonic Chick Heart Measured Using Optical Coherence Tomography". W ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176148.

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The heart is the first functional organ in the vertebrate embryo. In the chick embryo, the heart begins beating at Hamburger and Hamilton [1] stage 10 (approximately 35 hours of a 21-day incubation period). The initially straight heart tube bends and twists into a c-shaped tube before reaching stage 12 (approximately 48 hours incubation). This process, known as c-looping, marks one of the first visible signs of left-right asymmetry in the embryo. Incorrect looping is one cause of congenital heart defects, where significant malformations occur in roughly 1% of human live births [2]. Understanding the mechanisms that drive c-looping could lend insight into the processes causing some of these defects.
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Young, Jonathan M., Eric M. Beecher, Benjamen A. Filas, Larry A. Taber i Renato Perucchio. "FEM Voxel Modeling of the Tubular Embryonic Chick Heart for Finite Strain Analysis". W ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-192756.

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Significant progress has been made in the study of the developmental biomechanics of the embryonic chick heart through the use of the finite element method (FEM) [1, 2, 3]. Our work focuses on the geometry of the Hamburger-Hamilton stages 9–12 embryonic chick heart, approximately the time when the heart begins to function and undergoes drastic morphological changes, such as c-looping. Our objective is to devise a method for building an accurate 3D solid FEM mesh used for nonlinear analysis of the myocardium (MY) and cardiac jelly (CJ). The models are based on the extraction of voxels from optical coherence tomography (OCT) images of an arrested developing heart. To alleviate the problem of jagged edges introduced by the hexahedral voxel structure, we present a method for geometric smoothing and mesh coarsening. The performance of the voxel and smoothed models are tested given physiological loading conditions (pressure, biological growth, muscle contraction), to ascertain which model should be used for modeling the c-looping process.
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Ma, Zhenhe, Shidan Dou, Yuqian Zhao, Yi Wang, Yanyan Suo i Fengwen Wang. "Measurement of wall shear stress in chick embryonic heart using optical coherence tomography". W SPIE BiOS, redaktorzy Andrew M. Rollins, Scott E. Fraser i Michael A. Choma. SPIE, 2015. http://dx.doi.org/10.1117/12.2078832.

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Liu, Aiping, Ruikang Wang, Kent Thornburg i Sandra Rugonyi. "Wall Motion Influences Flow Pattern in the Outflow Tract of the Chick Embryonic Heart Tube". W ASME 2010 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/sbc2010-19484.

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The outflow tract (OFT) of the chick embryonic heart offers a good model system to study the association between blood flow dynamics and cardiac morphogenesis in early heart development. At early stages, the chick heart is a looped tube without valves. The OFT, the distal region of the heart, functions as a primitive valve [1]. The OFT is a slightly curved tube with three-layered wall (Fig. 1 (A) and (B)): the myocardium, an external muscle layer that actively contracts; the endocardium, an inner endothelial layer that directly contacts blood; in between the cardiac jelly, an extracellular matrix layer. The OFT undergoes complex morphogenesis, eventually leading to the development of semilunar valves, and this morphogenesis is sensitive to blood flow dynamics.
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Benslimane, Fatiha, Muneera Ahmed, Hissa Al-Thani, Maha Al Ser i Huseyin Yalcin. "Investigating the Effect of Hyperglycemia on Embryonic Heart Development using the Chick Embryo Model". W Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0137.

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Raporty organizacyjne na temat "Chick heart"

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Nielsen, Jan Erik. Collector fields – Check of performance. IEA SHC Task 55, grudzień 2020. http://dx.doi.org/10.18777/ieashc-task55-2020-0015.

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Yahav, Shlomo, John Brake i Noam Meiri. Development of Strategic Pre-Natal Cycling Thermal Treatments to Improve Livability and Productivity of Heavy Broilers. United States Department of Agriculture, grudzień 2013. http://dx.doi.org/10.32747/2013.7593395.bard.

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The necessity to improve broiler thermotolerance and live performance led to the following hypothesis: Appropriate comprehensive incubation treatments that include significant temperature management changes will promote angiogenesis and will improve acquisition of thermotolerance and carcass quality of heavy broilers through epigenetic adaptation. It was based on the following questions: 1. Can TM during embryogenesis of broilers induce a longer-lasting thermoregulatory memory (up to marketing age of 10 wk) that will improve acquisition of thermotolerance as well as increased breast meat yield in heavy broilers? 2. The improved sensible heat loss (SHL) suggests an improved peripheral vasodilation process. Does elevated temperature during incubation affect vasculogenesis and angiogenesis processes in the chick embryo? Will such create subsequent advantages for heavy broilers coping with adverse hot conditions? 3. What are the changes that occur in the PO/AH that induce the changes in the threshold response for heat production/heat loss based on the concept of epigenetic temperature adaptation? The original objectives of this study were as follow: a. to assess the improvement of thermotolerance efficiency and carcass quality of heavy broilers (~4 kg); b. toimproveperipheral vascularization and angiogenesis that improve sensible heat loss (SHL); c. to study the changes in the PO/AH thermoregulatory response for heat production/losscaused by modulating incubation temperature. To reach the goals: a. the effect of TM on performance and thermotolerance of broilers reared to 10 wk of age was studied. b. the effect of preincubation heating with an elevated temperature during the 1ˢᵗ 3 to 5 d of incubation in the presence of modified fresh air flow coupled with changes in turning frequency was elucidated; c.the effect of elevated temperature on vasculogenesis and angiogenesis was determined using in ovo and whole embryo chick culture as well as HIF-1α VEGF-α2 VEGF-R, FGF-2, and Gelatinase A (MMP2) gene expression. The effects on peripheral blood system of post-hatch chicks was determined with an infrared thermal imaging technique; c. the expression of BDNF was determined during the development of the thermal control set-point in the preoptic anterior hypothalamus (PO/AH). Background to the topic: Rapid growth rate has presented broiler chickens with seriousdifficulties when called upon to efficiently thermoregulate in hot environmental conditions. Being homeotherms, birds are able to maintain their body temperature (Tb) within a narrow range. An increase in Tb above the regulated range, as a result of exposure to environmental conditions and/or excessive metabolic heat production that often characterize broiler chickens, may lead to a potentially lethal cascade of irreversible thermoregulatory events. Exposure to temperature fluctuations during the perinatal period has been shown to lead to epigenetic temperature adaptation. The mechanism for this adaptation was based on the assumption that environmental factors, especially ambient temperature, have a strong influence on the determination of the “set-point” for physiological control systems during “critical developmental phases.” Recently, Piestunet al. (2008) demonstrated for the first time that TM (an elevated incubation temperature of 39.5°C for 12 h/d from E7 to E16) during the development/maturation of the hypothalamic-hypophyseal-thyroid axis (thermoregulation) and the hypothalamic-hypophyseal-adrenal axis (stress) significantly improved the thermotolerance and performance of broilers at 35 d of age. These phenomena raised two questions that were addressed in this project: 1. was it possible to detect changes leading to the determination of the “set point”; 2. Did TM have a similar long lasting effect (up to 70 d of age)? 3. Did other TM combinations (pre-heating and heating during the 1ˢᵗ 3 to 5 d of incubation) coupled with changes in turning frequency have any performance effect? The improved thermotolerance resulted mainly from an efficient capacity to reduce heat production and the level of stress that coincided with an increase in SHL (Piestunet al., 2008; 2009). The increase in SHL (Piestunet al., 2009) suggested an additional positive effect of TM on vasculogenesis and angiogensis. 4. In order to sustain or even improve broiler performance, TM during the period of the chorioallantoic membrane development was thought to increase vasculogenesis and angiogenesis providing better vasodilatation and by that SHL post-hatch.
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Bolek, Kevin J., i Michael E. Persia. The Effect of Chick Methionine Status on Broiler Performance and Physiological Response to Acute and Chronic Heat Stress. Ames (Iowa): Iowa State University, styczeń 2013. http://dx.doi.org/10.31274/ans_air-180814-821.

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Yahav, Shlomo, John McMurtry i Isaac Plavnik. Thermotolerance Acquisition in Broiler Chickens by Temperature Conditioning Early in Life. United States Department of Agriculture, 1998. http://dx.doi.org/10.32747/1998.7580676.bard.

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The research on thermotolerance acquisition in broiler chickens by temperature conditioning early in life was focused on the following objectives: a. To determine the optimal timing and temperature for inducing the thermotolerance, conditioning processes and to define its duration during the first week of life in the broiler chick. b. To investigate the response of skeletal muscle tissue and the gastrointestinal tract to thermal conditioning. This objective was added during the research, to understand the mechanisms related to compensatory growth. c. To evaluate the effect of early thermo conditioning on thermoregulation (heat production and heat dissipation) during 3 phases: (1) conditioning, (2) compensatory growth, (3) heat challenge. d. To investigate how induction of improved thermotolerance impacts on metabolic fuel and the hormones regulating growth and metabolism. Recent decades have seen significant development in the genetic selection of the meat-type fowl (i.e., broiler chickens); leading to rapid growth and increased feed efficiency, providing the poultry industry with heavy chickens in relatively short growth periods. Such development necessitates parallel increases in the size of visceral systems such as the cardiovascular and the respiratory ones. However, inferior development of such major systems has led to a relatively low capability to balance energy expenditure under extreme conditions. Thus, acute exposure of chickens to extreme conditions (i.e., heat spells) has resulted in major economic losses. Birds are homeotherms, and as such, they are able to maintain their body temperature within a narrow range. To sustain thermal tolerance and avoid the deleterious consequences of thermal stresses, a direct response is elicited: the rapid thermal shock response - thermal conditioning. This technique of temperature conditioning takes advantage of the immaturity of the temperature regulation mechanism in young chicks during their first week of life. Development of this mechanism involves sympathetic neural activity, integration of thermal infom1ation in the hypothalamus, and buildup of the body-to-brain temperature difference, so that the potential for thermotolerance can be incorporated into the developing thermoregulation mechanisms. Thermal conditioning is a unique management tool, which most likely involves hypothalamic them1oregulatory threshold changes that enable chickens, within certain limits, to cope with acute exposure to unexpected hot spells. Short-tem1 exposure to heat stress during the first week of life (37.5+1°C; 70-80% rh; for 24 h at 3 days of age) resulted in growth retardation followed immediately by compensatory growth" which resulted in complete compensation for the loss of weight gain, so that the conditioned chickens achieved higher body weight than that of the controls at 42 days of age. The compensatory growth was partially explained by its dramatic positive effect on the proliferation of muscle satellite cells which are necessary for further muscle hypertrophy. By its significant effect of the morphology and functioning of the gastrointestinal tract during and after using thermal conditioning. The significant effect of thermal conditioning on the chicken thermoregulation was found to be associated with a reduction in heat production and evaporative heat loss, and with an increase in sensible heat loss. It was further accompanied by changes in hormones regulating growth and metabolism These physiological responses may result from possible alterations in PO/AH gene expression patterns (14-3-3e), suggesting a more efficient mechanism to cope with heat stress. Understanding the physiological mechanisms behind thermal conditioning step us forward to elucidate the molecular mechanism behind the PO/AH response, and response of other major organs. The thermal conditioning technique is used now in many countries including Israel, South Korea, Australia, France" Ecuador, China and some places in the USA. The improvement in growth perfom1ance (50-190 g/chicken) and thermotolerance as a result of postnatal thermal conditioning, may initiate a dramatic improvement in the economy of broiler's production.
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Halevy, Orna, Zipora Yablonka-Reuveni i Israel Rozenboim. Enhancement of meat production by monochromatic light stimuli during embryogenesis: effect on muscle development and post-hatch growth. United States Department of Agriculture, czerwiec 2004. http://dx.doi.org/10.32747/2004.7586471.bard.

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The original objectives were: A. To determine the critical embryonic age for monochromatic green light stimulation. B. To follow the ontogeny of embryos exposed to monochromatic green light vs. darkness. C. To investigate the effects of monochromatic green light illumination on myoblast and fiber development in the embryo. D. To investigate the stimulatory effect of light combinations during embryo and post-hatch periods on growth and meat production. E. To evaluate the direct effect of monochromatic green light on cultured embryonic and adult myoblasts. The overall purpose of this study was to investigate the effect of monochromatic light stimuli during incubation period of broilers on muscle development and satellite cell myogenesis. Based on previous studies (Halevy et al., 1998; Rozenboim et al., 1999) that demonstrated the positive effects of green-light illumination on body and muscle growth, we hypothesized that monochromatic light illumination accelerates embryo and muscle development and subsequently enhances muscle growth and meat production. Thus, further decreases management costs. Under the cooperation of the laboratories at the Hebrew University of Jerusalem and University of Washington we have conducted the following: 1. We have established the critical stage for exposure to green monochromatic light which has the maximal effect on body and muscle growth (Objective A). We report that embryonic day 5 is optimal for starting illumination. The optimal regime of lighting that will eliminate possible heat effects was evaluated by monitoring egg core temperature at various illumination periods. We found that intermitted lighting (15 min. on; 15 min. off) is optimal to avoid heat effects. 2. We have evaluated in detail gross changes in embryo development profile associated to green light stimuli vs. darkness. In addition, we have investigated the stimulatory effect of light combinations during embryo and post-hatch periods on body and muscle growth (Objective B,D). 3. We have studied the expression profile of muscle regulatory proteins during chicken muscle cell differentiation in cultures using newly developed antibodies. This study paved the way for analyzing the expression of these proteins in our photo stimulation experiments (Objective C). 4. We have studied the pattern ofPax7 expression during myogenesis in the posthatch chicken. Experimental chick pectoralis muscles as well adult myoblast cultures were used in this study and the results led us to propose a novel model for satellite cell differentiation and renewal. 5. The effects of monochromatic green light illumination during embryogenesis have been studied. These studies focused on fetal myoblast and satellite cell proliferation and differentiation at pre- and posthatch periods and on the effects on the expression of muscle regulatory proteins which are involved in these processes. In addition, we have analyzed the effect of photo stimulation in the embryo on myofiber development at early posthatch (Objective C). 6. In follow the reviewers' comments we have not conducted Objective E. The information gathered from these studies is of utmost importance both, for understanding the molecular basis of muscle development in the posthatch chicks and for applied approach for future broiler management. Therefore, the information could be beneficial to agriculture in the short term on the one hand and to future studies on chick muscle development in the embryo and posthatch on the other hand.
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Berry, Terri-Ann, Jordan H. D. Chiswell, Shannon L. Wallis i Roger Birchmore. The Effect of Airtightness on Indoor Air Quality in Timber Houses in New Zealand. Unitec ePress, grudzień 2017. http://dx.doi.org/10.34074/ocds.92017.

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This two-part study considers the impact of airtightness based on volatile organic compounds (VOCs) only. Two identical buildings (test and control) were constructed in Auckland, New Zealand. The test building contained an Intello vapour check membrane to reduce air-flow and subsequent heat loss. Air change rates (air changes per hour, or ACH) were calculated from air-leakage rates in each of the buildings. Data analysis demonstrated that the VOC levels in the test house were consistently higher than those established in the control house.
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Halevy, Orna, Sandra Velleman i Shlomo Yahav. Early post-hatch thermal stress effects on broiler muscle development and performance. United States Department of Agriculture, styczeń 2013. http://dx.doi.org/10.32747/2013.7597933.bard.

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In broilers, the immediate post-hatch handling period exposes chicks to cold or hot thermal stress, with potentially harmful consequences to product quantity and quality that could threaten poultry meat marketability as a healthy, low-fat food. This lower performance includes adverse effects on muscle growth and damage to muscle structure (e.g., less protein and more fat deposition). A leading candidate for mediating the effects of thermal stress on muscle growth and development is a unique group of skeletal muscle cells known as adult myoblasts (satellite cells). Satellite cells are multipotential stem cells that can be stimulated to follow other developmental pathways, especially adipogenesis in lieu of muscle formation. They are most active during the first week of age in broilers and have been shown to be sensitive to environmental conditions and nutritional status. The hypothesis of the present study was that immediate post-hatch thermal stress would harm broiler growth and performance. In particular, growth characteristics and gene expression of muscle progenitor cells (i.e., satellite cells) will be affected, leading to increased fat deposition, resulting in long-term changes in muscle structure and a reduction in meat yield. The in vitro studies on cultured satellite cells derived from different muscle, have demonstrated that, anaerobic pectoralis major satellite cells are more predisposed to adipogenic conversion and more sensitive during myogenic proliferation and differentiation than aerobic biceps femoris cells when challenged to both hot and cold thermal stress. These results corroborated the in vivo studies, establishing that chronic heat exposure of broiler chicks at their first two week of life leads to impaired myogenicity of the satellite cells, and increased fat deposition in the muscle. Moreover, chronic exposure of chicks to inaccurate temperature, in particular to heat vs. cold, during their early posthatch periods has long-term effects of BW, absolute muscle growth and muscle morphology and meat quality. The latter is manifested by higher lipid and collagen deposition and may lead to the white striping occurrence. The results of this study emphasize the high sensitivity of muscle progenitor cells in the early posthatch period at a time when they are highly active and therefore the importance of rearing broiler chicks under accurate ambient temperatures. From an agricultural point of view, this research clearly demonstrates the immediate and long-term adverse effects on broiler muscling and fat formation due to chronic exposure to hot stress vs. cold temperatures at early age posthatch. These findings will aid in developing management strategies to improve broiler performance in Israel and the USA. BARD Report - Project4592 Page 2 of 29
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Rahman, Kazi, Grace Lee, Kristina Vine, Amba-Rose Atkinson, Michael Tong i Veronica Matthews. Impacts of climate change on health and health services in northern New South Wales: an Evidence Check rapid review. The Sax Institute, grudzień 2022. http://dx.doi.org/10.57022/xlsj7564.

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This rapid review investigated the effects of climate change on health and health services in northern NSW—a known ‘hotspot’ for natural disasters—over the next 10-20 years. It included 92 peer-reviewed articles and 9 grey literature documents, with 17% focused on Northern NSW. Climate change will cause both an increase in average temperatures and in extreme weather events and natural disasters. Impacts particularly affecting Northern NSW are expected to include increases and exacerbations of: mental illness; infectious diseases, including those transmitted by mosquitoes, water and food; heat-related illnesses; chronic diseases including respiratory and cardiac conditions; injuries; and mortality—with vulnerable groups being most affected. Demand for health services will increase, but there will also be disruptions to medication supply and service availability. A whole-of-system approach will be needed to address these issues. There are numerous gaps in the research evidence and a lack of predictive modelling and robust locally relevant data.
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Author, Not Given. Air-To-Water Heat Pumps with Radiant Delivery in Low Load Homes: Tucson, Arizona and Chico, California (Fact Sheet). Office of Scientific and Technical Information (OSTI), listopad 2013. http://dx.doi.org/10.2172/1114053.

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Yahav, Shlomo, John Brake i Orna Halevy. Pre-natal Epigenetic Adaptation to Improve Thermotolerance Acquisition and Performance of Fast-growing Meat-type Chickens. United States Department of Agriculture, wrzesień 2009. http://dx.doi.org/10.32747/2009.7592120.bard.

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: The necessity to improve broiler thermotolerance and performance led to the following hypothesis: (a) thethermoregulatory-response threshold for heat production can be altered by thermal manipulation (TM) during incubation so as to improve the acquisition of thermotolerance in the post-hatch broiler;and (b) TM during embryogenesis will improve myoblast proliferation during the embryonic and post-hatch periods with subsequent enhanced muscle growth and meat production. The original objectives of this study were as follow: 1. to assess the timing, temperature, duration, and turning frequency required for optimal TM during embryogenesis; 2. to evaluate the effect of TM during embryogenesis on thermoregulation (heat production and heat dissipation) during four phases: (1) embryogenesis, (2) at hatch, (3) during growth, and (4) during heat challenge near marketing age; 3. to investigate the stimulatory effect of thermotolerance on hormones that regulate thermogenesis and stress (T₄, T₃, corticosterone, glucagon); 4. to determine the effect of TM on performance (BW gain, feed intake, feed efficiency, carcass yield, breast muscle yield) of broiler chickens; and 5. to study the effect of TM during embryogenesis on skeletal muscle growth, including myoblast proliferation and fiber development, in the embryo and post-hatch chicks.This study has achieved all the original objectives. Only the plasma glucagon concentration (objective 3) was not measured as a result of technical obstacles. Background to the topic: Rapid growth rate has presented broiler chickens with seriousdifficulties when called upon to efficiently thermoregulate in hot environmental conditions. Being homeotherms, birds are able to maintain their body temperature (Tb) within a narrow range. An increase in Tb above the regulated range, as a result of exposure to environmental conditions and/or excessive metabolic heat production that often characterize broiler chickens, may lead to a potentially lethal cascade of irreversible thermoregulatory events. Exposure to temperature fluctuations during the perinatal period has been shown to lead to epigenetic temperature adaptation. The mechanism for this adaptation was based on the assumption that environmental factors, especially ambient temperature, have a strong influence on the determination of the “set-point” for physiological control systems during “critical developmental phases.” In order to sustain or even improve broiler performance, TM during the period of embryogenesis when satellite cell population normally expand should increase absolute pectoralis muscle weight in broilers post-hatch. Major conclusions: Intermittent TM (39.5°C for 12 h/day) during embryogenesis when the thyroid and adrenal axis was developing and maturing (E7 to E16 inclusive) had a long lasting thermoregulatory effect that improved thermotolerance of broiler chickens exposed to acute thermal stress at market age by lowering their functional Tb set point, thus lowering metabolic rate at hatch, improving sensible heat loss, and significantly decreasing the level of stress. Increased machine ventilation rate was required during TM so as to supply the oxygen required for the periods of increased embryonic development. Enhancing embryonic development was found to be accomplished by a combination of pre-incubation heating of embryos for 12 h at 30°C, followed by increasing incubation temperature to 38°C during the first 3 days of incubation. It was further facilitated by increasing turning frequency of the eggs to 48 or 96 times daily. TM during critical phases of muscle development in the late-term chick embryo (E16 to E18) for 3 or 6 hours (39.5°C) had an immediate stimulatory effect on myoblast proliferation that lasted for up to two weeks post-hatch; this was followed by increased hypertrophy at later ages. The various incubation temperatures and TM durations focused on the fine-tuning of muscle development and growth processes during late-term embryogenesis as well as in post-hatch chickens.
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