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Chao, Ying L. (Ying Liang). "Ultrastructure of Azotobacter vinelandii". Thesis, North Texas State University, 1993. https://digital.library.unt.edu/ark:/67531/metadc798461/.
Pełny tekst źródłaGoss, Steven Philip Allan. "Ultrastructure and Mitosis of Glaucosphaera vacuolata". W&M ScholarWorks, 1993. https://scholarworks.wm.edu/etd/1539625804.
Pełny tekst źródłaHudson, Liam. "Ultrastructure of the A-band unit cell in relaxed muscle". Thesis, Imperial College London, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.310340.
Pełny tekst źródłaRidden, John. "Studies on the cell biology of the human sebaceous gland". Thesis, University of Oxford, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.258034.
Pełny tekst źródłaFahlén, Jesper. "The cell wall ultrastructure of wood fibres : effects of the chemical pulp fibre line". Doctoral thesis, KTH, Fiber- och polymerteknik, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-129.
Pełny tekst źródłaQC 20101012
Fahlén, Jesper. "The cell wall ultrastructure of wood fibres : effects of the chemical pulp fibre line /". Stockholm : Fibre and Polymer technology, KTH, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-129.
Pełny tekst źródłaStander, Cornelia Steynberg. "An ultrastructural and light microscopic study of melanocyte differentiation in chick embryos". Master's thesis, University of Cape Town, 1991. http://hdl.handle.net/11427/27149.
Pełny tekst źródłaau, jeremy shaw@uwa edu, i Jeremy Shaw. "Biomineralisation processes in the radula teeth of the chiton Acanthopleura hirtosa (Mollusca: Polyplacophora)". Murdoch University, 2007. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20080703.163505.
Pełny tekst źródłaMerced-Alejandro, Amelia. "Evolution of stomata in mosses (Bryophyta): From molecules to form and function". OpenSIUC, 2015. https://opensiuc.lib.siu.edu/dissertations/1038.
Pełny tekst źródłaKamakura, Tsukasa. "Ultrastructural Maturation of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes in a Long-Term Culture". Kyoto University, 2015. http://hdl.handle.net/2433/199202.
Pełny tekst źródłaStevanic, Srndovic Jasna. "Ultrastructure of the Primary Cell Wall of Softwood Fibres Studied using Dynamic FT-IR Spectroscopy". Licentiate thesis, KTH, Fibre and Polymer Technology, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4614.
Pełny tekst źródłaThe primary cell wall is a complex multipolymer system whose composite structure has been mostly determined from chemical and biochemical studies. Although the primary cell wall serves a central role, with regard to the connective properties of fibres, knowledge about the interactions among the polymers, when it comes to the mechanical properties, is very limited. The physical properties of the polymers, i.e. their elastic and viscous deformations, as well as the ultrastructure of the polymers, i.e. the interactions among the polymers in the outer fibre wall layers that lead to this behaviour, are still not fully understood.
The aim of this study was to examine how the different wood polymers, viz. lignin, protein, pectin, xyloglucan and cellulose, interact in the outer fibre wall layers of the spruce wood tracheid. The initial objective was to separate an enriched primary cell wall material from a first stage TMP, by means of screening and centri-cleaning. From this material, consisting of the primary cell wall (P) and outer secondary cell wall (S1) materials, thin sheets were prepared and analysed using a number of different analytical methods. The major measuring technique used was dynamic Fourier transform infra-red (FT-IR) spectroscopy in combination with dynamic 2D FT-IR spectroscopy. This technique is based on the detection of small changes in molecular absorption that occur when a sinusoidally stretched sample undergoes low strain. The molecular groups affected by the stretching respond in a specific way, depending on their environment, while the unaffected molecular groups provide no response to the dynamic spectra, by producing no elastic or viscous signals. Moreover, the dynamic 2D FT-IR spectroscopy provides useful information about various intermolecular and intramolecular interactions, which influence the reorientability of functional groups in a polymer material.
Measurements of the primary cell wall material, using dynamic FT-IR spectroscopy, indicated that strong interactions exist among lignin, protein and pectin, as well as among cellulose, xyloglucan and pectin in this particular layer. This was in contrast to the secondary cell wall, where interactions of cellulose with glucomannan and of xylan with lignin were dominant. It was also indicated that the most abundant crystalline cellulose in the primary cell wall of spruce wood fibres is the cellulose Iβ allomorph, which was also in contrast to the secondary cell wall, where the cellulose Iα allomorph is more dominant. The presence of strong interactions among the polymers in the primary cell wall and, especially, the relatively high content of pectin and protein, showed that there is a very good possibility of selectively attacking these polymers in the primary cell wall. The first selective reaction chosen was a low degree of sulphonation, applied by an impregnation pretreatment of chips with a very low charge of sodium sulfite (Na2SO3). This selective reaction caused some structural modification of the lignin, a weakening of the interactions between lignin;pectin, lignin;protein and pectin;protein, as well as an increased softening of the sulphonated primary cell wall material, when compared to the unsulphonated primary cell wall material. All this resulted in an increased swelling ability of the material.
Stevanic, Srndovic Jasna. "Ultrastructure of the primary cell wall of softwood fibres studied using dynamic FT-IR spectroscopy /". Stockholm : Fiber- och polymerteknologi, Kungliga Tekniska högskolan, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4614.
Pełny tekst źródłaKokinos, John Peter. "Studies on the cell wall of dinoflagellate resting cysts : morphological development, ultrastructure, and chemical composition". Thesis, Massachusetts Institute of Technology, 1994. http://hdl.handle.net/1721.1/17366.
Pełny tekst źródłaMansouri, Katayoun. "COMPARATIVE ULTRASTRUCTURE OF APICAL CELLS AND DERIVATIVES IN BRYOPHYTES, WITH SPECIAL REFERENCE TO PLASMODESMATA". OpenSIUC, 2012. https://opensiuc.lib.siu.edu/dissertations/484.
Pełny tekst źródłaStanley, MacIsaac Sarah. "Ultrastructure of the visceral ganglion in the ascidian larva Ciona intestinalis, cell circuitry and synaptic distribution". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0017/MQ57329.pdf.
Pełny tekst źródłaUsher-Smith, Juliet Alexandra. "An in vitro reconstruction of the influence of exercise on skeletal muscle cell volume and ultrastructure". Thesis, University of Cambridge, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.612748.
Pełny tekst źródłaSchultes, Klaus. "Ultrastructural characterization of ultraviolet induced corneal disease : an animal model". Master's thesis, University of Cape Town, 1994. http://hdl.handle.net/11427/27046.
Pełny tekst źródłaSilva, Thiago Pereira da. "Bacteria from freshwater ecosystems: structural aspects and programmed cell death". Universidade Federal de Juiz de Fora (UFJF), 2017. https://repositorio.ufjf.br/jspui/handle/ufjf/6145.
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Bacteria are important components of the food web structure in aquatic ecosystems in which they influence the flow of carbon and energy. Populations of bacteria in these ecosystems comprise a diverse spectrum of individual cells able to respond to many factors such as nutrient supply, temperature and virus infection, which regulate bacterial life and death. Bacterial death is a key cellular event involved in the control and production of bacteria in aquatic ecosystems with functional meaning in the carbon and nutrient cycles. Therefore, the study of bacterial structural features and cellular mechanisms underlying bacterial death is crucial to understand processes affecting the entire population. However, both bacterial structure and cellular events of death in aquatic ecosystems are still poorly understood. In the present work, we used single cell approaches to study the structural organization of bacteria as well as to characterize cellular processes of death in these organisms. First, by using fluorescence and transmission electron microscopy (TEM), we provided a general panorama of how microscopy techniques, especially TEM, are powerful tools to understand bacterial structure and their responses to environmental stresses. We showed that bacteria from aquatic ecosystems have remarkable ultrastrutural diversity with components such as bacterial envelope of individual cells differing in structure within the same population. Second, we sought to identify and characterize mechanisms of bacterial cell death. Because our TEM analyses revealed morphological signs of apoptosis, a type of program cell death (PCD), in aquatic bacteria directly collected from natural ecosystems, we applied different techniques to detect apoptosis in bacteria cultured from natural samples. We used TEM as well as different probes to detect this type of PCD in cultured bacteria exposed to increased temperature and viral infection, which are recognized inducers of bacterial death. TEM showed, in both situations, ultrastructural changes indicative of apoptosis, such as cell retraction and condensation, similar to those reported for eukaryotic cells. Assays for membrane permeability, DNA fragmentation, phosphatidilserine exposition and caspase activation were significantly increased in treated bacteria compared to the control group. Altogether, our data demonstrate, for the first time, that PCD occur in aquatic bacteria, and that this event may be a basic mechanism for regulation of bacterial communities in these ecosystems.
Wester, Brock Andrew. "Development and characterization of mechanically actuated microtweezers for use in a single-cell neural injury model". Diss., Georgia Institute of Technology, 2011. http://hdl.handle.net/1853/39645.
Pełny tekst źródłaFremont, Patrick. "Differenciation en culture in vitro de myoblastes d'ebauches musculaires lentes et rapides de l'embryon d'oiseau : influence de l'innervation et de l'activite mecanique". Nantes, 1987. http://www.theses.fr/1987NANT2028.
Pełny tekst źródłaBerger, Bruno [UNESP]. "Controles do desenvolvimento ovariano em abelhas africanizadas adultas, Apis mellifera Linné, 1758 (Hymenoptera, Apidae)". Universidade Estadual Paulista (UNESP), 2010. http://hdl.handle.net/11449/100574.
Pełny tekst źródłaOs ovários das rainhas diferem dos de operárias de Apis mellifera quanto ao número e comprimento dos ovaríolos. Tanto o número, como o comprimento destes, é muito maior na rainha que nas operárias. No entanto, em ambos os casos os ovários são funcionais, isto é, capazes de produzir óvulos maduros. Apesar disso, operárias e rainhas diferem muito quanto à fertilidade e aos mecanismos controladores/estimuladores da vitelogênese, ou seja, da maturação dos óvulos. Em condições normais da colônia, nas rainhas a vitelogênese é desencadeada pelo acasalamento e nas operárias, pela ausência da rainha ou do recebimento de informações sobre sua presença na colônia. Passada a ocasião própria para o acasalamento no caso da rainha, e em idade avançada das operárias, os ovários entram em degeneração. O objetivo do presente trabalho foi o de avaliar como se comporta o desenvolvimento do ovário em rainhas e operárias mantidas fora dos condicionamentos da colônia e o efeito do tratamento destas com CO2, prática corrente na apicultura. Para tanto, operárias e rainhas foram mantidas aprisionadas em caixas com candy e água durante 15 dias e seguida a seqüência de desenvolvimento de seus ovários. O efeito do não acasalamento na época própria e do tratamento com CO2 foi feito estudando a morfologia do desenvolvimento da ovogênese, usando TUNEL e reação de fosfatase acida para caracterizar possíveis alterações celulares. As células do filamento terminal apresentaram-se empilhadas em fila única. Na transição para o germário as células tornam-se piramidais com a base apoiada sobre a lâmina própria e o ápice voltado para o centro do ovaríolo. São encontradas células esféricas, provavelmente ovogônias. No germário estão presentes células somáticas e germinativas, sendo da linhagem germinativa, os cistoblastos, os cistócitos, os ovócitos e as futuras...
The ovaries of queens and workers of Apis mellifera differs in number and length of the ovarioles. Length and number of ovarioles are larger in queen than in workers. However, in both cases, the ovaries are functional, i.e., it is able of produce mature eggs. Despite of that, workers and queens differ very in fertility and mechanisms of controlling/inducing vitellogenesis. In colony conditions, queen’s vitellogenesis is triggered by the matting and in workers by the absence of the queen or of the receipt of information about its presence in the colony. After the age proper to mate or in workers advanced age, the ovaries enter in degeneration. The objective of the present work was the evaluating of the ovary development in queens and workers maintained caged outside of the colony conditionings and the effect of the narcosis with CO2, practice current in the beekeeping. Newly emerged queens and workers were caged with candy and water during 15 days. For the queens the effect of the mate delay and CO2 narcosis were studied using TUNEL and acid fosfatase reaction to evaluate cell damages. The cells of the terminal filament appear as rows of one single cell, with a rectangular shape, poor in organelles and with a big central nucleus. In the transition for the germarium the cells present a pyramidal form with their base widened resting on tunica propria and the apex directed to center of the ovariole. Below the region of transition to the germarium are spherical cells, probably the oogonia. In the germarium are found somatic (pre-follicular cells) and germinative (cystoblasts, the cystocists, the oocytes and the future nurse cells) cells. The queen’s ovaries develop normally until the mating age, 5 days old queens. About the 10 days, the virgin queen beginning to presents an ovariolar disorganization with big incidence of injured cells with characteristics of apoptosis and autofagic death... (Complete abstract click electronic access below)
Berger, Bruno. "Controles do desenvolvimento ovariano em abelhas africanizadas adultas, Apis mellifera Linné, 1758 (Hymenoptera, Apidae) /". Rio Claro : [s.n.], 2009. http://hdl.handle.net/11449/100574.
Pełny tekst źródłaBanca: Karina Patrício
Banca: José Lino Neto
Banca: José Chaud Netto
Banca: Daniela Carvalho dos Santos
Resumo: Os ovários das rainhas diferem dos de operárias de Apis mellifera quanto ao número e comprimento dos ovaríolos. Tanto o número, como o comprimento destes, é muito maior na rainha que nas operárias. No entanto, em ambos os casos os ovários são funcionais, isto é, capazes de produzir óvulos maduros. Apesar disso, operárias e rainhas diferem muito quanto à fertilidade e aos mecanismos controladores/estimuladores da vitelogênese, ou seja, da maturação dos óvulos. Em condições normais da colônia, nas rainhas a vitelogênese é desencadeada pelo acasalamento e nas operárias, pela ausência da rainha ou do recebimento de informações sobre sua presença na colônia. Passada a ocasião própria para o acasalamento no caso da rainha, e em idade avançada das operárias, os ovários entram em degeneração. O objetivo do presente trabalho foi o de avaliar como se comporta o desenvolvimento do ovário em rainhas e operárias mantidas fora dos condicionamentos da colônia e o efeito do tratamento destas com CO2, prática corrente na apicultura. Para tanto, operárias e rainhas foram mantidas aprisionadas em caixas com candy e água durante 15 dias e seguida a seqüência de desenvolvimento de seus ovários. O efeito do não acasalamento na época própria e do tratamento com CO2 foi feito estudando a morfologia do desenvolvimento da ovogênese, usando TUNEL e reação de fosfatase acida para caracterizar possíveis alterações celulares. As células do filamento terminal apresentaram-se empilhadas em fila única. Na transição para o germário as células tornam-se piramidais com a base apoiada sobre a lâmina própria e o ápice voltado para o centro do ovaríolo. São encontradas células esféricas, provavelmente ovogônias. No germário estão presentes células somáticas e germinativas, sendo da linhagem germinativa, os cistoblastos, os cistócitos, os ovócitos e as futuras... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The ovaries of queens and workers of Apis mellifera differs in number and length of the ovarioles. Length and number of ovarioles are larger in queen than in workers. However, in both cases, the ovaries are functional, i.e., it is able of produce mature eggs. Despite of that, workers and queens differ very in fertility and mechanisms of controlling/inducing vitellogenesis. In colony conditions, queen's vitellogenesis is triggered by the matting and in workers by the absence of the queen or of the receipt of information about its presence in the colony. After the age proper to mate or in workers advanced age, the ovaries enter in degeneration. The objective of the present work was the evaluating of the ovary development in queens and workers maintained caged outside of the colony conditionings and the effect of the narcosis with CO2, practice current in the beekeeping. Newly emerged queens and workers were caged with candy and water during 15 days. For the queens the effect of the mate delay and CO2 narcosis were studied using TUNEL and acid fosfatase reaction to evaluate cell damages. The cells of the terminal filament appear as rows of one single cell, with a rectangular shape, poor in organelles and with a big central nucleus. In the transition for the germarium the cells present a pyramidal form with their base widened resting on tunica propria and the apex directed to center of the ovariole. Below the region of transition to the germarium are spherical cells, probably the oogonia. In the germarium are found somatic (pre-follicular cells) and germinative (cystoblasts, the cystocists, the oocytes and the future nurse cells) cells. The queen's ovaries develop normally until the mating age, 5 days old queens. About the 10 days, the virgin queen beginning to presents an ovariolar disorganization with big incidence of injured cells with characteristics of apoptosis and autofagic death... (Complete abstract click electronic access below)
Doutor
Lemullois, Michel. "Etude ultrastructurale et immunocytochimique de la ciliogenese dans l'oviducte de caille". Paris 6, 1988. http://www.theses.fr/1988PA066360.
Pełny tekst źródłaSantana, Júlio César de Oliveira 1985. "Relações suprafamiliares em Erythrinoidea (Teleostei: Characiformes) com base em caracteres moleculares e da morfologia das células espermáticas = Suprafamilial relationships of the Erythrinoidea (Teleostei: Characiformes) based on molecular and spermatic cell data". [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317601.
Pełny tekst źródłaTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: A superfamília Erythrinoidea é uma das oito famílias que compõem a ordem Characiformes e foi proposta como um grupo monofilético para abrigar as famílias neotropicais Ctenoluciidae, Erythrinidae e Lebiasinidae, e a família africana Hepsetidae. Após a proposição da superfamília, estudos filogenéticos recentes com base em caracteres morfológicos, como osteologia e morfologia de partes moles, e caracteres moleculares tem refutado a ideia de monofiletismo do grupo. Além disso, diferentes autores divergem quanto às relações suprafamiliares de Erythrinoidea. Além dos caracteres tradicionais, o emprego dos caracteres oriundos da análise da ultraestrutura da ontogenia dos espermatozoides e sua forma final tem sido uma importante fonte na complementação dos estudos filogenéticos. O estudo apresentado teve como objetivo realizar uma análise filogenética para testar a monofiletismo de Erythrinoidea e verificar os padrões de relacionamento suprafamiliares em Characiformes, e testar o posicionamento filogenético da família Crenuchidae, supostamente relacionada à superfamília. As análises filogenéticas foram realizadas com base em dados moleculares e dados morfológicos independentemente, e de maneira conjunta (evidência total) através do método de Parcimônia. A ultraestrutura da espermiogênese e dos espermatozoides dos gêneros incluídos em Erythrinoidea, mais a família Crenuchidae e outras possivelmente relacionadas, foram descritos e codificados em uma matriz de dados. Nas três análises realizadas a superfamília Erythrinoidea não é recuperada como monofilética. A família Crenuchidae posiciona-se na base da subordem Characoidei e não está estreitamente relacionada à Erythrinoidea. As análises com dados moleculares e de evidência total apresentaram uma topologia muito semelhante ao nível mais inclusivo e os valores de suporte dos principais clados apresentaram-se maiores na evidência total, mostrando a influência positiva da inserção dos caracteres da morfologia das células espermáticas nas análises filogenéticas
Abstract: The superfamily Erythrinoidea is one of eight superfamilies within the order Characiformes and was proposed to include a monophyletic group composed of the Neotropical fish families Ctenoluciidae, Erythrinidae and Lebiasinidae, and African family Hepsetidae. Current phylogenetic studies based on morphological data, such as osteology and morphology of soft structures, and molecular data have refuted the hypothesis of monophyly of this group. In addition, the suprafamilial relationships in Erythrinoidea have diverged among the authors. In combination with more traditional characters, the analyses of the ultrastructure of sperm ontogeny and its final shape have become an interesting source of characters to complement phylogenetic studies. The goal of this study was to perform a phylogenetic analysis to test the monophyly of the Erythrinoidea and to assess the phylogenetic position of the family Crenuchidae, a group supposed to be closely related to the superfamily. The phylogenetic analyses were performed using molecular and morphological data separately, and in a combined approach (total evidence) through the Parsimony method. The ultrastructure of the spermiogenesis and the sperm shape of Erythrinoidea genera, plus the family Crenuchidae and other species possibly related to the superfamily were described and coded in a matrix data. In all three analyses Erythrinoidea was not recovered as monophyletic. The family Crenuchidae was placed in a basal position within the suborder Characoidei in molecular and total evidence analysis, and was not closely related to the Erythrinoidea. The analyses using only molecular data and total evidence showed a very similar topology for the more inclusive relationship levels. The support values were higher for the total evidence tree when compared to the one resulting of only molecular data possibly pointing to a positive influence of the combination of the spermatic cell data in phylogenetic analyses
Doutorado
Biologia Celular
Doutor em Biologia Celular e Estrutural
Menzel, Lorenzo P. "Aspects of the Innate Immune System in the Caribbean Octocoral Swiftia exserta". FIU Digital Commons, 2013. http://digitalcommons.fiu.edu/etd/1025.
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Pełny tekst źródłaLelong-Rebel, Isabelle. "Etude de l'evolution de l'architecture membranaire des neurones d'embryons de poulet en culture : composition et topologie des proteines et des lipides polaires au cours du developpement cellulaire". Université Louis Pasteur (Strasbourg) (1971-2008), 1989. http://www.theses.fr/1989STR13089.
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Pełny tekst źródłaPERRIN-WALDEMER, CLAUDE GILBERT. "Etude des glandes accessoires du male de drosophila melanogaster (meigen) : cytophysiologie et cytochimie". Clermont-Ferrand 2, 1987. http://www.theses.fr/1987CLF2E359.
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Pełny tekst źródłaPatrone, Laura May. "Ultrastructural Analysis of Vegetative and Mitotic Cells of the Unicellular Red Alga Rhodella violacea". W&M ScholarWorks, 1988. https://scholarworks.wm.edu/etd/1539625447.
Pełny tekst źródłaDorgans, Kevin. "Ultrastructural, molecular and functional heterogeneities of cerebellar granule cell presynaptic terminals". Thesis, Strasbourg, 2017. http://www.theses.fr/2017STRAJ083/document.
Pełny tekst źródłaCerebellum is a brain structure involved in motor regulation and motor learning. In the cerebellar cortex, sensorimotor information is transmitted by granule cells. During my PhD, I demonstrated that the properties of individual granule cell synaptic connections are highly heterogeneous. From one synapse to another, I observed ultrastructural, molecular and functional variability at unitary contacts. More precisely, I assessed the properties of short term plasticity at individual synapses during high frequency trains of stimulation :1) Short term plasticities are highly heterogeneous from one synapse to another and can be classified in sub-categories.2) Some categories of short-term plasticity profiles relie on the expression of molecules such as Synapsin2.3) The response of post-synaptic neuron to high-frequency inputs is dependent on the nature of the activated synaptic contact
Moreira-de-Sousa, Cristina. "Avaliação dos impactos gerados pela vinhaça bruta e após ajuste de pH, em representantes da fauna edáfica". Universidade Estadual Paulista (UNESP), 2018. http://hdl.handle.net/11449/158310.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
A cana-de-açúcar é uma das principais culturas mundialmente difundida e a alta produtividade resulta na geração de inúmeros resíduos. A vinhaça, resíduo da produção do etanol tem chamado a atenção devido suas características e propriedades, quando empregada na fertirrigação das culturas de cana-de-açúcar. Diversos benefícios foram descritos, ganhos na fertilização e enriquecimento do solo, bem como aumentos na produtividade. Entretanto, a vinhaça também apresenta substâncias que podem ser nocivas, afetando negativamente a fauna existente nos locais de aplicação do resíduo. Frente a essa problemática, diversos estudos foram desenvolvidos com o intuito de melhor compreender os impactos da vinhaça no meio ambiente e, mesmo diante dos benefícios que a fertirrigação com a vinhaça implica economicamente ainda há a necessidade de maiores cuidados com a utilização do resíduo no campo. Nesse contexto, o objetivo deste trabalho foi propor um tratamento para a vinhaça, ajustando seu pH em 7,0 (neutro) utilizando cal (CaO), com a intenção de amenizar sua toxicidade para posterior uso no solo, visto que, um dos grandes problemas apresentados pela vinhaça é o pH ácido. O uso de cal foi escolhido por ser este produto utilizado em campo para correção do solo. Foi associado o uso de diversos biomarcadores à bioindicadores de solo, além de testes ecotoxicológicos para avaliar o efeito da vinhaça bruta em comparação à vinhaça tratada. Diplópodos da espécie Rhinocricus padbergi foram expostos à vinhaça em sua forma bruta e tratada com CaO, na concentração estabelecida pela Norma da CETESB, e ao dobro desta mesma concentração, simulando uma situação de super dosagem. A análise do intestino médio destes animais por meio das ferramentas ultraestruturais, imunohistoquímica e marcação de morte celular revelou que a vinhaça bruta pode ocasionar danos nos tecidos dos animais expostos e que o tratamento desta vinhaça surtiu efeito na diminuição desses danos. Testes ecotoxicológicos de fuga e reprodução, padronizados mundialmente pela Organização Internacional de Normalização (ISO), foram realizados com as espécies Eisenia andrei, Enchytraeus crypticus e Folsomia candida; de um modo geral, observou-se que as espécies E. crypticus e F. candida não tiveram seus comportamentos influenciados pela vinhaça bruta e nem pela vinhaça tratada, mas a espécie E. andrei apresentou-se mais sensível a presença da vinhaça no solo demonstrando comportamento de fuga em concentrações mais elevadas de vinhaça bruta e tratada, e, no teste de reprodução, respondeu a exposição à vinhaça tratada, com aumento no número de juvenis em relação a vinhaça bruta. Logo, o emprego da vinhaça na fertirrigação ainda requer cuidados, uma vez que seus efeitos nocivos são notórios, e nesse sentido, a alternativa de neutralizar seu pH pode representar uma medida de emprego desse resíduo com menos impacto.
Sugarcane is one of the main crops worldwide and the high productivity results in the generation of many wastes. Vinasse, the residue of ethanol production, has attracted attention because of its characteristics and properties, when used in the fertirrigation of sugarcane crops. Several benefits have been described, gains in fertilization and soil enrichment, as well as increases in productivity. However, the vinasse also presents substances that can be harmful, negatively affecting the fauna existing in the places of application of the residue. Faced with this problem, several studies were developed with the purpose of better understanding the impacts of vinasse in the environment and, even in view of the benefits that fertirrigation with vinasse implies economically, there is still a need for greater care with the use of the residue in the field. In this context, the objective of this work was to propose a treatment for vinasse, adjusting its pH to 7.0 (neutral) using lime (CaO), with the intention of mitigating its toxicity for later use in the soil, since one of the great problems presented by vinasse is acid pH. The use of lime was chosen because this product is used in field for soil correction. It was associated the use of several biomarkers to soil bioindicators, as well as ecotoxicological tests to evaluate the effect of raw vinasse in comparison to the treated vinasse. Diplopods of the species Rhinocricus padbergi were exposed to vinasse in their raw form and treated with CaO, at the concentration established by the CETESB Standard, and at twice the same concentration, simulating a super dosage situation. The analysis of the midgut of these animals using ultrastructural tools, immunohistochemistry and cell death marking revealed that raw vinasse can cause damage to the tissues of the exposed animals and that the treatment of this vinasse had an effect in reducing these damages. Ecotoxicological tests of the avoidance and reproduction tests, standardized worldwide by the International Organization for Standardization (ISO), were carried out with the species Eisenia andrei, Enchytraeus crypticus and Folsomia candida; in general, E. crypticus and F. candida were not influenced by raw vinasse or treated vinasse, but the E. andrei species was more sensitive to the presence of vinasse in the soil, demonstrating in the higher concentrations of raw and treated vinasse, and in the reproduction test, the exposure to treated vinasse responded, with an increase in the number of juveniles in relation to raw vinasse. Therefore, the use of vinasse in fertigation still requires care, since its harmful effects are notorious, and in this sense, the alternative of neutralizing its pH can represent a measure of the use of this residue with less impact.
CAPES: 001
Daniels, Alison. "Microscopic and computer analysis of ultrastructural changes accompanying isolation and manipulation of tobacco protoplasts". Thesis, University of Nottingham, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.278755.
Pełny tekst źródłaChoi, Jiwon 1977. "The role of fibulin-5 : in the ultrastructural and biomechanical properties of skin". Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=81611.
Pełny tekst źródłaBairaktaris, George. "Ultrastructural investigation of matrix and cell surface factors in corneal transplantation, refractive surgery and stem-cell grafting". Thesis, Lancaster University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.340515.
Pełny tekst źródłaDamerval, Marc. "Identification et rôle physiologique des inclusions contenues dans le système nerveux central de la moule Mytilus edulis et de la crépidule Crepidula fornicata". Caen, 1985. http://www.theses.fr/1985CAEN2004.
Pełny tekst źródłaMak, Kai-yu, i 麥啟宇. "Substratum effects of micro- and nano-structures on cellular behavior". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/202233.
Pełny tekst źródłapublished_or_final_version
Electrical and Electronic Engineering
Master
Master of Philosophy
Beswetherick, John T. "An ultrastructural study of host and non-host resistance reactions in plant cells". Thesis, Open University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292658.
Pełny tekst źródłaMutasa, H. C. F. "Cytology, cytochemistry and ultrastructure of blood cells with special reference to myeloid leukaemias". Thesis, University of Cambridge, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335133.
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