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Artykuły w czasopismach na temat "Cell-Scattering correction"

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Gray, E. MacA, R. I. Smith i M. P. Pitt. "Time-of-flight neutron powder diffraction with a thick-walled sample cell". Journal of Applied Crystallography 40, nr 3 (15.05.2007): 399–408. http://dx.doi.org/10.1107/s0021889807006668.

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The time-of-flight diffraction techniques that are normally practiced at pulsed neutron sources afford opportunities that are not readily available at continuous fixed-wavelength sources. The present work concerns the increasing trend in materials science to study samples in complex non-ambient environments, such as high gas pressure. Taking the example of a sample cell in which a material is studied under fluid pressure, the optimization of the cell design for best data collection rate is considered. The design of primary- and scattered-beam masks for eliminating background scattering from the sample cell and the correction of the data for cell and sample attenuation are addressed. The outputs of this work include a simple expression for the optimum wall thickness of a thick-walled sample cell, a procedure for accurately determining the required mask aperture width for any scattering angle, more compact expressions for some of the results of the work of Paalman & Pings [J. Appl. Phys.(1962),33, 2635–2639] on absorption corrections, and guidance as to the correction of diffraction profiles for cell and background effects. Examples are given, drawn from studies of materials under hydrogen gas pressures up to 1800 bar in cells constructed from Ti2.1Zr and Inconel.
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Nettesheim, Florian, Ulf Olsson, Peter Lindner i Walter Richtering. "Correction method for the asymmetry of the tangential beam in Couette (or Searle) geometry used in rheo-small-angle neutron scattering". Journal of Applied Crystallography 37, nr 3 (11.05.2004): 438–44. http://dx.doi.org/10.1107/s0021889804006910.

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A method of correcting the asymmetry in the scattering of the tangential beam configuration in a rheo-small-angle neutron scattering experiment is proposed. The asymmetry of the scattering in the tangential beam configuration can be attributed to the difference in pathlength for neutrons that are scattered toward compared with those which are scattered away from the axis of rotation of the shear cell. The pathlength problem is solved and a final expression for the two-dimensional scattering intensity is given. The results from these calculations are compared with experimental data, which offer a different option to correct this asymmetry, namely by just measuring the scattering of H2O/D2O mixtures with absolute scattering cross sections identical to those of the respective samples. However, the situation for anisotropic media is more complex and the correction procedure described here is less effective.
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Kohler, A., J. Sulé-Suso, G. D. Sockalingum, M. Tobin, F. Bahrami, Y. Yang, J. Pijanka i in. "Estimating and Correcting Mie Scattering in Synchrotron-Based Microscopic Fourier Transform Infrared Spectra by Extended Multiplicative Signal Correction". Applied Spectroscopy 62, nr 3 (marzec 2008): 259–66. http://dx.doi.org/10.1366/000370208783759669.

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We present an approach for estimating and correcting Mie scattering occurring in infrared spectra of single cells, at diffraction limited probe size, as in synchrotron based microscopy. The Mie scattering is modeled by extended multiplicative signal correction (EMSC) and subtracted from the vibrational absorption. Because the Mie scattering depends non-linearly on α, the product of the radius and the refractive index of the medium/sphere causing it, a new method was developed for estimating the Mie scattering by EMSC for unknown radius and refractive index of the Mie scatterer. The theoretically expected Mie contributions for a range of different α values were computed according to the formulae developed by Van de Hulst (1957). The many simulated spectra were then summarized by a six-dimensional subspace model by principal component analysis (PCA). This subspace model was used in EMSC to estimate and correct for Mie scattering, as well as other additive and multiplicative interference effects. The approach was applied to a set of Fourier transform infrared (FT-IR) absorbance spectra measured for individual lung cancer cells in order to remove unwanted interferences and to estimate ranges of important α values for each spectrum. The results indicate that several cell components may contribute to the Mie scattering.
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Kato, Minoru, i Tetsuro Fujisawa. "High-Pressure Solution X-ray Scattering of Protein Using a Hydrostatic Cell with Diamond Windows". Journal of Synchrotron Radiation 5, nr 5 (1.09.1998): 1282–86. http://dx.doi.org/10.1107/s0909049598000788.

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A hydrostatic high-pressure cell (maximum pressure 700 MPa) with synthetic diamond windows is applied to measure small-angle X-ray scattering of a protein at high pressure. Use of the present cell allows an accurate solvent background correction, providing quantitative analyses. The performance of the present cell for X-ray scattering is shown by using lysozyme as a sample solution. From the Guinier plot, values of the radius of gyration of lysozyme are evaluated to be 15.31 ± 0.09 Å at 1 atm (0.10 MPa) and 14.80 ± 0.15 Å at 400 MPa.
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Kang, Joon Ho, Teemu P. Miettinen, Lynna Chen, Selim Olcum, Georgios Katsikis, Patrick S. Doyle i Scott R. Manalis. "Publisher Correction: Noninvasive monitoring of single-cell mechanics by acoustic scattering". Nature Methods 16, nr 3 (19.02.2019): 270. http://dx.doi.org/10.1038/s41592-019-0354-6.

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Roth, M. "On absolute scaling in protein crystallography using sums of low-resolution intensities and Wilson statistics at low resolution". Acta Crystallographica Section A Foundations of Crystallography 42, nr 4 (1.07.1986): 230–40. http://dx.doi.org/10.1107/s0108767386099373.

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A method of absolute scaling of diffraction data is proposed, based on the calculation of the sum of the intensity diffracted at low resolution (Bragg d spacing > 15 Å). This sum is proportional to the mean-square deviation of the scattering-length density in the unit cell, and this property is used to determine the scale factor. The method is applied to the case of neutron diffraction using contrast variation experiments with biological molecules, and it is used to check the validity of some assumptions concerning the system under study, such as the global rate of H/D exchange or the uniformity of scattering-length density in the molecules. The use of this method requires an asymptotic correction of the sum of intensity. This correction is based on Porod's law, whose application to diffraction experiments is discussed, in particular for contrast variation experiments. An analysis of the spherical average of the diffracted intensity as a function of the scattering vector, compared to isotropic solution scattering, allows the conditions of applicability of Wilson statistics to be specified at low and medium resolution, i.e. the random statistical model underlying the Wilson statistics in this scattering range to be defined.
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Hariri, Sara, Sahar Barzegari B., Kamyar Keshavarz F., Nastaran Nikounezhad, Behnoosh Safaei, Golrokh Farnam i Farshad H. Shirazi. "FTIR bio-spectroscopy scattering correction using natural biological characteristics of different cell lines". Analyst 144, nr 19 (2019): 5810–28. http://dx.doi.org/10.1039/c9an00811j.

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The Mie type scattering makes the FTIR spectra of biological samples unreliable. Here, natural biological characteristics have been used instead of mathematical estimations to solve the scattering problem, which resulted the best corrected spectra of cells.
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Hoogerheide, David P., Frank Heinrich, Brian B. Maranville i Charles F. Majkrzak. "Accurate background correction in neutron reflectometry studies of soft condensed matter films in contact with fluid reservoirs". Journal of Applied Crystallography 53, nr 1 (1.02.2020): 15–26. http://dx.doi.org/10.1107/s160057671901481x.

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Neutron reflectometry (NR) is a powerful method for looking at the structures of multilayered thin films, including biomolecules on surfaces, particularly proteins at lipid interfaces. The spatial resolution of the film structure obtained through an NR experiment is limited by the maximum wavevector transfer at which the reflectivity can be measured. This maximum is in turn determined primarily by the scattering background, e.g. from incoherent scattering from a liquid reservoir or inelastic scattering from cell materials. Thus, reduction of scattering background is an important part of improving the spatial resolution attainable in NR measurements. Here, the background field generated by scattering from a thin liquid reservoir on a monochromatic reflectometer is measured and calculated. It is shown that background subtraction utilizing the entire background field improves data modeling and reduces experimental uncertainties associated with localized background subtraction.
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Wheater, R. M., M. D. Hart, M. C. Veale, M. D. Wilson, D. Doblas-Jiménez, M. Turcato, C. Milne, H. Yousef i D. Khakhulin. "Development of data correction for the 1M Large Pixel Detector at the EuXFEL". Journal of Instrumentation 17, nr 04 (1.04.2022): P04013. http://dx.doi.org/10.1088/1748-0221/17/04/p04013.

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Abstract We present the development of a gain correction process for the Large Pixel Detector (LPD) developed by the Science and Technology Facilities Council (STFC) for the Femtosecond X-Ray Experiment (FXE) instrument based at the European X-ray Free Electron Laser (EuXFEL). LPD operates at 4.5 MHz as necessitated by EuXFEL's pulse timing structure and has a large dynamic range capturing a maximum of 105 photons/pixel @ 12 keV. Experiments at FXE require highly accurate measurements of relative changes in X-ray scattering intensity on the MHz timescale making a correction bringing LPD pixel outputs in line against a common axis essential to understanding experimental results. To facilitate the operational needs of LPD at FXE more than 1.5 billion correction coefficients are required, with corrections generated on a per pixel, per memory cell and per gain stage basis. By comparing output LPD signals to an independent reference diode a linear gain correction has been developed and applied to LPD data. Improvements in image quality have been quantified using the standard deviation in the measured intensity per pixel for a given radius from the beam centre. Improvements to detector uniformity of up to 30.0% are demonstrated in the medium gain stage and 15.9% in the high gain stage. This paper presents an in-depth view of the gain correction development process and further results.
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Cacocciola, Riccardo, Badreddine Ratni, Nicolas Mielec, Emmanuel Mimoun i Shah Nawaz Burokur. "Metasurfaces for Far-Field Radiation Pattern Correction of Antennas under Dielectric Seamed-Radomes". Materials 15, nr 2 (16.01.2022): 665. http://dx.doi.org/10.3390/ma15020665.

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A high-index dielectric radome seam is camouflaged with respect to a low-index dielectric radome panel by tuning the seam with carefully engineered metasurfaces. A transmission-line approach is used to model the metasurface-tuned seam and analytically retrieve the corresponding surface impedance, from which the unit-cell design is then tailored. Full-wave simulations and microwave antenna measurements performed on a proof-of-concept prototype validate the undesired scattering suppression effect in the case of normally and obliquely incident transverse electric and transverse magnetic wave illuminations. Robustness of the proposed solution to fabrication tolerances is also reported. The study presents metasurface-tuning as an easily implementable, frequency adjustable, and polarization insensitive solution to reduce the scattering of dielectric mechanical seams and improve the overall transparency performance of radome structures.
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Rozprawy doktorskie na temat "Cell-Scattering correction"

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Yang, Ning. "Online monitoring of bioreactors by Raman spectroscopy and machine learning". Electronic Thesis or Diss., université Paris-Saclay, 2024. http://www.theses.fr/2024UPAST083.

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Cette thèse présente une nouvelle stratégie de modélisation pour le suivi en ligne des bioréacteurs, utilisant la spectroscopie Raman et Machine Learning. L'objectif principal de cette étude est de développer des modèles simplifiés utilisant des spectres Raman provenant d'étalons. Elle se compose de trois parties.La première partie de cette thèse consiste à optimiser les paramètres d'acquisition Raman et à élaborer un modèle de régression PLS en utilisant des étalons purs. Ensuite, une validation préliminaire est entreprise en employant des étalons mixtes afin de simuler les variations de composition moléculaire dans le milieu, au cours du procédé, la fermentation alcoolique. La deuxième partie propose une expression non linéaire pour interpréter l'atténuation Raman due à la présence de micro-organismes dans un bioréacteur réel, permettant ainsi la correction des spectres diffusés par les cellules. Pour évaluer la performance du modèle, des bioréacteurs batch et fed-batch ont été réalisés, afin de confirmer la fiabilité et la robustesse prédictive de la stratégie de correction et du modèle de régression élaborés. Enfin, la troisième partie met en lumière les avantages de la méthodologie de modélisation proposée, comparativement à l'approche traditionnelle utilisant les spectres des bioréacteurs pour l'entraînement du modèle de régression.Dans l'ensemble, cette approche innovante a démontré une excellente performance de prédiction sur tous les ensembles de données de validation et de test, présentant un potentiel significatif pour l'ingénierie des bioprocédés. Elle permet un suivi plus précis et plus efficace de plusieurs composés en temps réel, et améliore le contrôle et l'optimisation des processus. La stratégie proposée devrait avoir une application étendue dans l'industrie de la bioproduction
This thesis presents a novel and reproducible modeling strategy for online monitoring of bioreactors using Raman spectroscopy and Machine Learning. The main aim of this study is to develop simplified models using the Raman spectra of standards in solution. It consists of three key parts.The first part involves optimizing Raman acquisition parameters and developing a PLS regression model using pure standards. Subsequently, a preliminary validation was carried out using mixed standards to mimic changes in the composition of different molecules in the medium during the alcoholic fermentation process. The second part defined a nonlinear expression to interpret the Raman attenuation induced by the presence of microorganisms in a real bioreactor, allowing the correction of cell--scattered spectra. For model evaluation, numerous batches and one fed--batch bioreactor were launched to validate the working performance and predictive robustness of the obtained correction strategy and regression model. The third part highlights the advantages of our proposed modeling methodology over the traditional way that uses the spectra from bioreactors to train the regression model.Overall, the innovative approach demonstrated an excellent prediction performance on all validation and testing datasets, presenting significant potential for bioprocess engineering. It enables more accurate and efficient monitoring of multiple compounds in real time, as well as enhances process control and optimization. The proposed strategy is expected to have an extended application in the bioproduction industry
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Części książek na temat "Cell-Scattering correction"

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Honkimaki, V., i P. Suortti. "Effects of instrument function, crystallite size, and strain on reflection profiles". W Defect and Microstructure Analysis by Diffraction, 41–58. Oxford University PressOxford, 2000. http://dx.doi.org/10.1093/oso/9780198501893.003.0004.

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Abstract Abstract A new approach to the analysis of reflection profiles is introduced. The basic idea is to calculate the instrument function by ray-tracing and include in the whole-pattern fitting a background function of the correct dependence on the scattering vector. In this way, only the effects of crystallite size and strain remain to be modeled. The instrument function is presented in general form, which can be adapted to energy-dispersive or angle-dispersive powder diffraction. The effects of the source size and wavelength distribution, equatorial and axial divergences, beam penetration in the sample, and different slits are included. Most of these aberrations are independent and can be cast in analytical forms, and the total instrument function is found by successive convolutions. The background arising from inelastic and resonant scattering is calculated from theory, while disorder scattering is modeled by a pair-correlation function and thermal diffuse scattering (TDS) by the Debye model for acoustic phonons and by the Einstein model for optical phonons. The total TDS is calculated as the part of the Bragg reflections lost due to thermal motion. The effects of crystallite size are treated in a general formalism where the reflection profile is the Fourier transform of the common volume of the crystallite and its ‘ghost’ shifted a distance tin the direction of the scattering vector k.The effects of strain are included in a distribution function of unit-cell displacements. Long-distance strain is described by a characteristic function, and the local strain by a correction term to the structure factor. Formulae that cover transition from local strain to long-distance strain are given. Anisotropic crystallite size and strain are described in terms of spherical harmonics which obey the crystal symmetry. The diffraction pattern of PbBr2 measured using CuKa1radiation is resolved using the procedure described above. The model parameters include only the thermal motion amplitudes and coefficients of the spherical harmonics that give the anisotropic crystallite size and strain.
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Streszczenia konferencji na temat "Cell-Scattering correction"

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He, Qingming, Hongchun Wu, Yunzhao Li, Liangzhi Cao i Tiejun Zu. "Neutron Up-Scattering Effect in Refined Energy Group Structure". W 2016 24th International Conference on Nuclear Engineering. American Society of Mechanical Engineers, 2016. http://dx.doi.org/10.1115/icone24-60313.

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Aiming at generating a 361-group library, this paper investigated neutron up-scattering effect in the 361-group Santamarina-Hfaiedh Energy Mesh (SHEM). Firstly, the Doppler Broadening Rejection Correction (DBRC) method is implemented to consider the neutron up-scattering effect in Monte Carlo (MC) method. Then the MC method is employed to prepare resonance integral table and scattering matrix for afterward calculation. Numerical results show that the neutron up-scattering affects kinf by ∼200 pcm at most for UO2 pin cell problems in the 361-group SHEM, while the fuel temperature coefficient (FTC) is also influenced by 12∼13%. It has also been found that both of the above two influences acts through scattering matrix rather than self-shielded absorption cross sections. In addition, the self-shielding effect of cladding is studied and it’s been found that it affects kinf by 30∼70 pcm.
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Dlabal, M. L., J. Reintjes i R. H. Lehmberg. "High-fidelity phase conjugation of broadband laser radiation with stimulated Brillouin scattering using a grating disperser". W OSA Annual Meeting. Washington, D.C.: Optica Publishing Group, 1986. http://dx.doi.org/10.1364/oam.1986.tui3.

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We describe the use of a grating disperser to allow effective phase conjugation of broadband radiation with stimulated Brillouin scattering. With conventional configurations the bandwidth of the pump radiation is restricted because the Brillouin reflectivity decreases when the pump bandwidth becomes wider than the Brillouin linewidth and the conjugate fidelity decreases when the coherence length becomes shorter than the effective interaction length. With our technique the pump beam is focused into a laterally extended focal region, each element of which has effectively narrowband radiation. As long as these narrowband focal-region elements carry sufficient spatial information each will provide a conjugate of the pump wave at its appropriate wavelength. Because the return beam from the Brillouin cell is a conjugate of the incoming beam it will reconstruct off the grating to form a conjugate of the beam as a whole, rather than a beam with double the dispersion. We demonstrate the performance of this technique in correcting phase aberrations using radiation at 353 nm from a XeF laser and SBS in n-hexane. Comparisons are made of performance with and without the grating disperser and results are compared with theory. In addition we present studies of the effects of various types and degrees of aberration and various amounts of grating dispersion.
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