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Data publikacji: 25 maja 2024

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1

Zheng, Yao. "Identification of interacting mitochondrial enzymes involved in pyrroline-5-carboxylate metabolism in Arabidopsis thaliana". Electronic Thesis or Diss., Sorbonne université, 2021. https://accesdistant.sorbonne-universite.fr/login?url=https://theses-intra.sorbonne-universite.fr/2021SORUS269.pdf.

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La proline, acide aminé protéinogène, joue un rôle crucial dans le métabolisme cellulaire. Dans les mitochondries, la proline est oxydée en glutamate par l'action séquentielle de la proline déshydrogénase (ProDH) et de la pyrroline-5-carboxylate (P5C) déshydrogénase (P5CDH). L'ornithine-δ-aminotransférase (δOAT) participe également à la formation de P5C via la conversion de l'ornithine et de l’α -cétoglutarate en glutamate et P5C. L’utilisation de mutants et d’approches biochimiques a révélé que ProDH1, P5CDH et δOAT sont impliquées dans la sénescence des feuilles induite à l'obscurité (DIS) chez Arabidopsis thaliana. Une accumulation importante de P5C et de proline est observée chez le mutant p5cdh et, dans une moindre mesure chez le mutant prodh1prodh2, suggérant un cycle proline-P5C. Les mutants prodh1prodh2 et p5cdh ont un profil métabolomique similaire qui diffère de celui du WT et de oat, démontrant ainsi le rôle de l'oxydation de la proline au cours de la sénescence. Nous avons montré que ProDH1 est essentiellement associée à la membrane mitochondriale, tandis que P5CDH et δOAT sont plus uniformément réparties entre la matrice et la membrane. L’oligomérisation de ProDH1, P5CDH et δOAT a été révélée à l'aide d’une analyse de complémentation bimoléculaire de fluorescence (BiFC). Les interactions entre ces enzymes du métabolisme du P5C ont été confirmées par des approches de protéomique couplée à la MS en condition de sénescence chez A. thaliana. Ces trois enzymes forment un(des) complexe(s) impliqué(s) dans l'oxydation de la proline pour alimenter la chaîne de transfert d'électrons mitochondriale afin de pourvoir aux besoins énergétiques des cellules sénescentes
The proteinogenic amino acid proline plays a crucial role for cellular metabolism in living organisms. In mitochondria, proline is oxidized to glutamate by the sequential action of proline dehydrogenase (ProDH) and pyrroline-5-carboxylate (P5C) dehydrogenase (P5CDH). In addition, ornithine δ-aminotransferase (δOAT) also participates in P5C formation through the conversion of ornithine and α-ketoglutarate into glutamate and P5C. Using mutants and biochemical approaches, ProDH1, P5CDH and δOAT were shown to be involved during dark-induced leaf senescence (DIS) in Arabidopsis thaliana. Striking accumulation of P5C and proline was observed in p5cdh mutant and to a lesser extent in prodh1prodh2 mutant, suggesting a putative proline-P5C cycle. Metabolomic analysis indicated that prodh1prodh2 and p5cdh have a similar metabolomic profile, but significantly different from wild-type and oat mutant, demonstrating the role of proline oxidation during DIS. ProDH1 was shown to be preferentially associated to the mitochondrial membrane fraction, while P5CDH and δOAT are more evenly distributed between matrix and membrane fractions. Homo- and hetero-oligomerizations of ProDH1, P5CDH, and δOAT were revealed using Bimolecular Fluorescence Complementation (BiFC) assay of infiltrated tobacco leaves. Interactions between P5C metabolism enzymes were further highlighted in DIS leaves using proteomics approaches coupled with mass spectrometry. Our work demonstrates that these three enzymes form P5C metabolic complex(es) involved in the oxidation of proline to fuel mitochondrial electron transfer chain to support the energy needs of senescent cells
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2

Hsueh, Li-Ching. "Studies on proline hydroxylases". Thesis, University of Oxford, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.365805.

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3

Küllchen, Felix B. "Studies on proline 3-hydroxylase". Thesis, University of Oxford, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.269221.

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4

Schulz, Daniel [Verfasser]. "L-Proline and Glutamatergic Neurotransmission : Clarifying the Modulatory Role of Neuronal L-Proline Transporter / Daniel Schulz". Bonn : Universitäts- und Landesbibliothek Bonn, 2012. http://d-nb.info/1044971096/34.

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5

Lawrence, Christopher C. "Studies on bacterial proline 4-hydroxylase". Thesis, University of Oxford, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.358610.

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6

Hu, Chien-an Andy. "Osmoregulation and proline biosynthesis in plants /". The Ohio State University, 1993. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487843688956923.

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7

Townsend, David E. Wilkinson Brian J. "Proline transport and biosynthesis in Staphylococcus aureus". Normal, Ill. Illinois State University, 1992. http://wwwlib.umi.com/cr/ilstu/fullcit?p9311291.

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Thesis (Ph. D.)--Illinois State University, 1992.
Title from title page screen, viewed February 6, 2006. Dissertation Committee: Brian J. Wilkinson (chair), Radheshyam Jayaswal, Herman E. Brockman, Robert L. Preston, Philip D. Morse. Includes bibliographical references (leaves 107-112) and abstract. Also available in print.
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8

Le, Dinh Thien. "Métabolisme de la proline chez les mammifères". Paris 5, 1988. http://www.theses.fr/1988PA05P216.

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9

Habibi-Najafi, Mohammad B. (Mohammad Bagher). "Proline-specific peptidases from Lactobacillus casei subspecies". Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=28455.

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The objectives of this study were (l) to screen out active proline-specific peptidases from Lactobacillus casei subspecies, (2) to study growth kinetic and enzyme production from enriched medium (MRS) and cheese whey medium, (3) to purify and characterize two active proline-specific enzymes, and (4) to investigate the action of purified enzyme on bitter tryptic digests of $ beta$-casein as well as bitter enzyme-modified cheese. Lactobacillus casei subsp. casei LLG and Lactobacillus casei subsp. rhamnosus S93 were examined for extra- and intra-cellular proline-specific peptidase activities. Both strains showed strong activity for x-prolyl dipeptidyl peptidase and proline iminopeptidase but had weak activities for prolidase, prolinase, and post proline endopeptidase. Histochemical staining of crude enzyme extract from Lactobacillus casei ssp. casei LLG with different substrates revealed a distinct protein band for x-prolyl dipeptidyl peptidase as well as for proline iminopeptidase. The growth kinetics showed that the intracellular proline-specific peptidases increased gradually at the beginning of the exponential phase and reached a maximum at the beginning of stationary phase.
Storage stability of x-prolyl dipeptidyl peptidase and proline iminopeptidase in crude extract, with and without stabilizers showed no significant loss in activity of these two enzymes at 4$ sp circ$C for 9 days without adding any stabilizers. The levels of x-prolyl dipeptidyl peptidase, proline iminopeptidase, and post proline endopeptidase activities of cells grown in whey did not vary markedly from cells grown in MRS broth. X-prolyl dipeptidyl peptidase and proline iminopeptidase were purified from crude cell-free extract of Lactobacillus casei ssp. casei LLG by Fast Protein Liquid Chromatography (FPLC) equipped with ion-exchange and gel-filtration columns. X-prolyl dipeptidyl peptidase was found to be a serine-dependent enzyme with molecular mass of 79 kDa. The pH and the temperature optima by the purified enzyme were 7.0 and 50$ sp circ$C, respectively. Proline iminopeptidase was sulfhydryl enzyme with molecular mass of 46 kDa. The maximum enzyme activity was observed at pH 7.5 and 40$ sp circ$C. This is the first report describing the purification and characterization of x-prolyl dipeptidyl peptidase and proline iminopeptidase from Lactobacillus casei to homogeneity.
The debittering of tryptic digests from $ beta$-casein by x-prolyl dipeptidyl peptidase was studied by reversed phase high performance liquid chromatography (RP-HPLC) and liquid chromatography/mass spectrometry. The results showed that two bitter peptides (f53-97 and f03-209) containing X-Pro-Y-Pro in their amino acid residues were completely hydrolyzed and many other peptides with high hydrophobicity were decreased in peak area. The addition of purified x-prolyl dipeptidyl peptidase on bitter enzyme-modified cheese (EMC) also showed that at least one bitter peptide with X-Pro-Y derived from $ alpha$-casein hydrolysis was removed.
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10

Walters, Nicola Jane. "Arginine and proline catabolism in Schizosaccharomyces pombe". Thesis, University of Cambridge, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.257192.

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11

Robinson, Kenneth J. "Bio-organic studies on proline 4-hydroxylase". Thesis, University of Oxford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320660.

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12

Jones, Iwan Gwynedd. "Desymmetrization of meso-anhydrides by proline esters". Thesis, Bangor University, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.265515.

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13

Daly, D. J. "Post-proline cleaving enzymes in skeletal muscle". Thesis, University of Newcastle Upon Tyne, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.304040.

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14

D'Rozario, Robert S. G. "Conformational dynamics of proline-containing transmembrane helices". Thesis, University of Oxford, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.670181.

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15

Pike, Claire Victoria Sarah. "The proline isomerase FKBP25 as a chromatin modifier". Thesis, University of Cambridge, 2010. https://www.repository.cam.ac.uk/handle/1810/252175.

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16

Verbruggen, Nathalie. "Proline accumulation after salt-stress in arabidopsis thaliana". Doctoral thesis, Universite Libre de Bruxelles, 1992. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/212895.

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17

Dikic, Inga. "Signal Transduction by Proline-Rich Tyrosine Kinase Pyk2". Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2002. http://publications.uu.se/theses/91-554-5316-3/.

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18

Norris, Simon Richard. "Studies towards the synthesis of useful proline derivatives". Thesis, University of Southampton, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315897.

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19

Bess, Kirstin. "Transcriptional repression by the proline rich homeodomain protein". Thesis, University of Bristol, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.390994.

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20

Omar, Muhiadin. "Biotransformations of proline by 2-oxoglutarate-dependent hydroxylases". Thesis, University of York, 2017. http://etheses.whiterose.ac.uk/19532/.

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Hydroxylases introduce hydroxyl groups with excellent regio- and enantioselectivity making them of significant interest for use in the production of pharmaceutical intermediates and drug metabolites. 2-oxoglutarate dependent oxygenases (2OGDOs) are non-haem dependent Fe(II) containing enzymes that catalyse various oxidation reactions, including the hydroxylation of free amino acids. Unlike the more studied cytochromes P450, these enzymes only require molecular oxygen, Fe(II) and 2-oxoglutarate for catalysis, circumventing the need for a costly cofactor regeneration system. The targets of this work were three proline hydroxylases: a trans-4-proline hydroxylase from Dactylsporangium sp. RH1 (DOGDH), a cis-3-proline-hydoxylase from Streptomyces sp. (StP3H) and a cis-4-proline-hydroxylase from Mesorhizobium loti (MlC4H). Genes encoding all three were cloned into the pET-YSBLIC3C (and pET22b for DOGDH) expression vectors, expressed in Escherichia coli, and produced and purified by chromatography for use in crystallisation studies and enzymatic transformations. Extensive crystallisation trials were attempted for DOGDH including enzymatic, chemical and mutagenic modification with little success. A homology model was therefore constructed in order to identify catalytic residues within the active site that could be manipulated for enhancing the function of DOGDH. A precolumn derivatisation assay using FMOC-Cl was developed for the analysis of proline and its hydroxylated equivalents by HPLC and LC-MS. Biotransformations were performed with L-proline using the three hydroxylases with whole cell reaction conditions deemed optimal due to the multi-component nature of the enzymes, with the cell providing machinery for the recycling of cofactors. Reactions were scaled from shake flasks to stirred tank vessels with the flow of air into the vessel and stirring rate deemed key parameters for optimal function. Finally, a high-throughput substrate screening method using a BioLecter micro-bioreactor was successfully developed and trialled with the three hydroxylases with a panel of substrates providing a platform for future investigations.
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21

Shibasaki, Takeshi. "SCREENING AND APPLIED STUDIES OF MICROBIAL PROLINE HYDROXYLASES". Kyoto University, 2001. http://hdl.handle.net/2433/150792.

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22

White, Tommi Anna. "Structural and functional studies of proline catabolic enzymes". Diss., Columbia, Mo. : University of Missouri-Columbia, 2007. http://hdl.handle.net/10355/4760.

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Thesis (Ph.D.)--University of Missouri-Columbia, 2007.
The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on March 24, 2009) Vita. Includes bibliographical references.
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23

UEDA, Akihiro, Weiming SHI, Takiko SHIMADA, Hiroshi MIYAKE i Tetsuko TAKABE. "Altered expression of barley proline transporter causes different growth responses in Arabidopsis". Springer, 2007. http://hdl.handle.net/2237/8780.

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24

Eriksen, Heidi Sofie. "The synthesis and use of polymer supported β amino sulfur containing catalysts". Thesis, University of Strathclyde, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.248596.

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25

Nguyen, Thi-Huong. "Synthèse de nouveaux catalyseurs chiraux à base de la L-proline. Applications en catalyse asymétrique". Thesis, Paris 11, 2014. http://www.theses.fr/2014PA112382.

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Depuis de nombreuses années, les phosphines chirales multifonctionnelles se sont révélées être des outils synthétiques puissants en organocatalyse asymétrique. Ces catalyseurs qui contiennent un site de base de Lewis et un site d'acide de Bronsted, ont reçu une attention particulière en raison de leur efficacité pour créer des liaisons C-C avec de très bonnes énantiosélectivités. A notre connaissance, la synthèse d'organocatalyseurs de type thiourée-phosphine dérivés de la (L)-proline n'a jamais été décrite dans la littérature. Ce sujet de thèse concerne la synthèse d'une nouvelle famille d'organocatalyseur chiral de type thiourée-phosphine dérivée de (L)-proline, un produit naturel, disponible et peu coûteux. Nous avons mis au point plusieurs méthodes de synthèse efficaces qui nous ont permis de préparer trois familles de phosphines-thiourées à partir de la (L)- proline. Ainsi, sept nouveaux composés énantiopurs ont été préparés au cours de ce travail. Ces composés ont été utilisés comme catalyseurs dans des réactions asymétriques catalysées par des phosphines. Ces réactions incluent la cyclisation [3+2], la réaction de Baylis-Hillman, la réaction de Friedel-Crafts, la réaction de substitution nucléophile
For many years, multifunctional chiral phosphines have proven to be powerful synthetic tools in asymmetric organocatalysis. These catalysts, containing Lewis basic and Brnsted acidic sites, have received considerable attention owing to their highly efficiency to create C-C bond by asymmetric organocatalysis. To our knowledge, the synthesis of organocatalysts type thiourea-phosphine derivatives (L) -proline have never been described in the literature. In this work, we wish to report the synthesis of new family of bifunctional chiral thiourea-phosphine organocatalyst derived from L-proline, a natural available product. We developed efficient methods to prepare three families of phosphine thiourea derived from L-proline. Thus, Seven new enantiopure compounds were synthesized in this study. They were used as catalyst asymmetric reaction catalyzed by phosphines: [3+2] cyclisation, Baylis-Hillman reaction, Friedel-Crafts reaction and nucleophilic substitution
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26

Picard, François J. "Genetics of arginine and proline biosynthesis in Neisseria gonorrhoeae". Thesis, University of Ottawa (Canada), 1991. http://hdl.handle.net/10393/7669.

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The carAB operon from E. coli hybridized with the gonococcal clones carrying carA or carB genes under conditions of high stringency (i.e. detecting approximately 80% or greater similarity) suggesting that the nucleotide sequence of the carbamoylphosphate synthetase genes is very similar in these two organisms. Under these conditions for hybridization, the gonococcal clones carrying argB or argF genes did not hybridize with plasmids containing the corresponding E. coli gene. Hybridizations performed under conditions of lower stringency indicate that the nucleotide sequence of the argB gene is less conserved than that of argF in E. coli and N. gonorrhoeae. Co-complementation experiments established gene linkage only between carA and carB and between proA and proB gonococcal genes. Clones complementing a gene defect in argE were also able to complement an argA mutation. This suggests that the enzyme ornithine acetyltransferase from N. gonorrhoeae (encoded by argJ) may be able to complement both argA and argE mutations in E. coli. The prevalence of specific arginine biosynthesis gene defects was studied for 319 arginine-requiring clinical isolates of Neisseria gonorrhoeae by using the ability of the strains to utilize intermediates of arginine biosynthesis. Nearly 99% of the strains were defective either in the conversion of acetylornithine to ornithine (174 strains) or in the conversion of ornithine to citrulline (141 strains). Based on a nutritional requirement for carbamoyl phosphate, only 11% of the uracil-requiring strains defective in the carbamylation of ornithine to yield citrulline were apparently defective in carAB. Three argininosuccinate-requiring strains (i.e. probably defective in argG) of auxotype PAU were identified. A high polymorphism was observed in hybridization patterns of restricted genomic DNA from N. gonorrhoeae strains having the same auxotype and serotype with a gonococcal CPSase gene-specific probe suggesting that this probe may provide a useful epidemiological marker for N. gonorrhoeae. Some of the arginine auxotrophs of N. gonorrhoeae defective in carAB, argJ, argF or argG were complemented by genetic transformation with DNA from recombinant bacteriophages carrying characterized gonococcal arginine biosynthesis genes. Gene defects in proA (5 strains) and in proB (6 strains) were identified by gonococcal transformation assays with recombinant bacteriophages or plasmids carrying proline biosynthesis genes from N. gonorrhoeae. None of the eleven proline-requiring strains tested appears to be defective in proC. Polymerase chain reaction (PCR) amplifications using oligonucleotides specific to conserved areas of the E. coli carAB operon yielded amplified copies of various portions of the N. gonorrhoeae CPSase genes. Amplifications using primers specific to the duplicated region of the E. coli carB gene suggest that the gonococcal carB gene contains a similar duplication. (Abstract shortened by UMI.)
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27

Digennaro, Angela [Verfasser]. "Proline-Quaterthiophene hybrids: Synthesis and self-assembly / Angela Digennaro". Ulm : Universität Ulm, 2017. http://d-nb.info/112490249X/34.

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28

Wood, Nicholas James. "The role of proline in osmoregulation by a streptomycete". Thesis, University of Warwick, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.319812.

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29

Murray, Nicola Jane. "NMR studies of salivary proline-rich proteins and tannins". Thesis, University of Sheffield, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.284595.

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30

Swingler, Tracey. "Transcriptional repression by the proline rich homeodomain protein (PRH)". Thesis, University of Bristol, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.419215.

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31

Roosens, Nancy. "Proline biosynthesis related to salt stress in higher plants". Doctoral thesis, Universite Libre de Bruxelles, 1999. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/211926.

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32

Obayashi, Yoko. "Novel physiological function of proline and mTOR regulator tuberin". Kyoto University, 2018. http://hdl.handle.net/2433/232154.

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33

Aillard, Boris. "Small molecule mimics of trans-proline : synthesis and applications". Thesis, University of Southampton, 2014. https://eprints.soton.ac.uk/367097/.

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Herein we detail the synthesis and application of small molecules of trans-proline mimics. A general introduction to the field of peptidomimetics and their uses in specific biological examples is provided; followed by a detailed review of the background and objectives of the project. The synthesis of trans-proline mimics using (–)-cytisine as a building block is disclosed. Its application is demonstrated by incorporation of (–)-cytisine derivatives in a specific anti-cancer peptide PRGPRP and determination of its anti-cancer activity. The synthesis and conformational analysis of a pyroglutamate based mimic, a trans-proline mimic designed to adopt a polyproline type II (PPII) helical conformation, is described. Incorporation of this mimic in the anti-cancer peptide PRGPRP and determination of its anti-cancer activity displays a useful application. To investigate the conformation of the pyroglutamic based mimic, synthesis and conformational analysis of its oligomers is detailed. Using circular dichroism (CD) spectroscopy, NMR and X-ray crystallographic structures, the PPII conformation of these oligomers are compared to the ideal PPII helices and known PPII mimics. A specific protein-ligand interaction (SH3-peptide ligand) is discussed. Incorporation of the pyroglutamic based mimic in the peptide sequence and its binding properties are disclosed. As a result of these studies, a second generation pyroglutamate based mimic is currently under development in the group to further investigate conformational analysis and binding properties with SH3.
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34

Voss, Emelyne. "Synthèse d’Analogues Bis-azotés de la Proline et Applications". Thesis, Vandoeuvre-les-Nancy, INPL, 2011. http://www.theses.fr/2011INPL059N/document.

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La liaison peptidique au sein d’un peptide ou d’une protéine est d’ordinaire plane et en conformation trans pour la majorité des acides aminés. La situation est un peu différente en amont d’une proline : la barrière thermodynamique qui s’oppose à la rotation de la liaison amide est plus faible et la tendance de la liaison à rester plane est un peu moins grande. Cette liaison AA-Pro peut donc adopter une conformation cis, entraînant la formation d’un coude prononcé dans une chaîne peptidique à son niveau. Ce travail décrit la synthèse et la réactivité chimique de nouveaux analogues bis-azotés de la proline en solution permettant de favoriser la conformation cis d’une liaison AA-ΨPro. L’impact conformationnel que peut engendrer ces résidus au sein de pseudopeptides est également exposé. Dans un premier temps, une nouvelle voie d’accès à la α-azaproline énantiomériquement pure et orthogonalement protégée a été mise au point en exploitant des travaux antérieurs concernant la synthèse d’α-hydrazinoesters et de N-aminodipeptides. L’étude de la réactivité de cette pseudoproline a permis de définir les meilleures conditions de formations de pseudotripeptides de formule P1-AA1-δ-azaPro-AA3-P3. Elle a également orienté les travaux, dans un second temps, vers la synthèse de pseudopeptides incorporant un motif acide pyrazolique. Enfin, la structure des composés préparés a été analysée par RMN, IR et par modélisation moléculaire. L’examen des P1-AA1-δ-azaPro(Boc)-AA3-P3 a révélé la formation par liaison hydrogène d’un pseudocycle en C7, favorisant la conformation trans de la liaison AA1-δ-azaPro, alors que l’absence de la fonction Boc favorise la conformation cis de cette liaison
The peptidic bond in a peptide or a protein is usually flat and in trans conformation for the majority of amino acids. The situation is a little bit different upstream the proline: the thermodynamic barrier which opposes the rotation of the amide bond is weaker and the tendency of the bond to remain flat is lesser. So, this AA-Pro bond can adopt a cis conformation, leading to the formation of a turn in the peptidic chain. This work describes the synthesis and the chemical reactivity of new bis-nitrogen analogous of proline in solution to facilitate the cis conformation of a AA-PΨPro bond. The conformational impact that these residues may generate in pseudopeptides is also exposed.Initially, a new access road to the orthogonally protected and enantiomerically pure δ-azaproline has been developed by exploiting previous work on the synthesis of α- hydrazinoesters and N-aminodipeptides. The study of the reactivity of this pseudoproline helped define the best conditions for forming pseudotripeptides of formula P1-AA1--δ-azaPro-AA3-P3. It also guided the work, in a second step, towards the synthesis of pseudopeptide incorporating a pyrazole acid motif. Finally, the structure of the prepared compounds was analyzed by NMR, IR and molecular modeling. Examination of the P1-AA1-δ-azaPro(Boc)-AA3-P3 revealed the formation of a pseudocycle C7 by a Hydrogen bond, favoring the trans conformation of the AA1-δ-azaPro bond, while the absence of Boc function seems to favor the cis conformation of this bond
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35

Zhang, Min. "Crystallographic studies of the E. coli puta proline dehydrogenase domain /". free to MU campus, to others for purchase, 2004. http://wwwlib.umi.com/cr/mo/fullcit?p1426119.

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36

Lilley, Ian Andrew. "Synthetic studies using chiral stabilised azomethine ylids". Thesis, University of Oxford, 1995. https://ora.ox.ac.uk/objects/uuid:64d5745b-e6ef-4a77-8abe-b01f1c627e8f.

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The work described herein is concerned with the [3+2] dipolar cycloaddition of amino acid derived azomethine ylids. Such cycloadditions are a highly efficient technique for the construction of proline derivatives, and may potentially be employed in other areas of asymmetric synthesis. Chapter 1 commences with a brief review of natural and synthetic, proline containing, molecules. Approaches to the synthesis of the proline moiety are described, focusing on previously developed methods for performing the [3+2] dipolar cycloaddition in a chiral manner. The methodology employed in this thesis is subsequently detailed, along with a brief description of the aims of the work. This is followed by a review of α-amino acid synthesis via chiral template technology. The potential application of chiral [3+2] dipolar cycloadditions to such syntheses is introduced. Chapter 2 describes the cycloaddition of carboxylate substituted chiral azomethine ylids with a range of dipolarophiles under both thermal and Lewis acid catalysed conditions. The effect on the stereoselectivity and yield caused by changing the conditions is discussed. Subsequent removal of the chiral template allows the synthesis of some tetra-substituted proline derivatives. Chapter 3 details the intramolecular variant of the cycloaddition. Further functionalisation of the cycloadducts via insertion of alternative chain links and sulfone alkylation was attempted. The Pummerer rearrangement of the related sulfoxide was shown to proceed smoothly and with total regio- and stereocontrol. Application of the methodology to the synthesis of a simple proline derivative and a symmetric pyrrolidine is described. Chapter 4 reports the attempted application of [3+2] dipolar cycloadditions to the synthesis of α-amino acids. The synthesis and subsequent cycloaddition of a new, α-amino acid derived, chiral template is described. Subsequent deprotection of the cycloadducts generated allows the synthesis of some α-phenyl substituted prolines. Unsuccessful attempts to incorporate additional substituents and perform the cycloaddition in an intramolecular manner are described. Chapter 5 contains a description of the techniques employed, together with spectroscopic data for the compounds described in this thesis.
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37

Touchal, Samira. "Synthèse de nouveaux matériaux polymères hydrosolubles : application à la séparation de mélanges de solvants organiques de type alcool / éther par pervaporation". Vandoeuvre-les-Nancy, INPL, 2003. http://docnum.univ-lorraine.fr/public/INPL_T_2003_TOUCHAL_S.pdf.

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Ce mémoire concerne la conception et la synthèse de films réticulés d’homo et de copolymères de N-vinylpyrrolidinone et de méthyl sulfate de N-[3-(N,N,N-triméthyl ammonium) méthacrylamide, films destinés à l’étude de la séparation par pervaporation de mélanges de liquides de type alcool/éther. Les homopolymères et les copolymères obtenus ont de fortes masses molaires. Les rapports de réactivité, calculés en tenant compte de l’avancement de la réaction, montrent une dérive très rapide de la composition initiale du mélange : inversement à la NVP, le monomère acrylamide a une forte tendance à homopolymériser. Le degré de miscibilité des homopolymères s’est revélé satisfaisant pour permettre la préparation de films transparents ayant des propriétés mécaniques et physico-chimiques satisfaisantes pour les études de sorption isotherme et de pervaporation. En faisant varier la structure et la composition des films, à base d’homopolymères ou de copolymère, les fortes affinités des matériaux pour les alcools ont été mises en lumière. De façon générale, pour un film donné, l’activité de l’alcool détermine l’amplitude des phénomènes observés et corrélativement la sorption d’éther est fortement dépendante de l’activité de l’alcool, ce qui correspond à un phénomène de synergie de sorption. Par ailleurs, il a été montré que les motifs acrylamides quaternisés limitaient fortement l’amplitude des phénomènes de sorption isotherme en phase vapeur comme en phase liquide ; des résultats concordant ont été obtenus en pervaporation. Pour les systèmes binaires, les meilleures modélisations des isothermes de sorption sont réalisées par les modèles ENSIC et UNIQUAC ; il a aussi été possible de modéliser la sorption du système ternaire éhtanol-tertiobutyl éthyl éhter. Dans le film formé à partir du copolymère statistique, comparativement aux films de mélanges d’homopolymères, une capacité de sorption d’éthanol plus forte a été notée en relation avec l’augmentant de la polarité moyenne de la structure. Enfin en faisant varier la nature des alcools et des éthers, il apparaît que le taux d’alcool sorbé est plus bas en présence d’éther peu polaire. Comparativement aux résultats de la littérature, les perfomances du transport pervaporatif sont comparables ou supérieures.
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38

Leung, Ka Wing. "PRiMA, a membrane anchor of tetrameric acetylcholinesterase (AChE), directs the restricted localization of the enzyme in muscle and neuron /". View abstract or full-text, 2009. http://library.ust.hk/cgi/db/thesis.pl?BIOL%202009%20LEUNG.

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39

Mok, Ka Wai. "Proline-rich membrane anchor (PRiMA) of acetylcholinesterase (AChE) : characterization of its splicing variants and their expression profiles in different chicken tissues /". View abstract or full-text, 2009. http://library.ust.hk/cgi/db/thesis.pl?BIOL%202009%20MOK.

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40

Luo, Bo. "Isomérisation de cycle d'azéphanes polyhydroxylés : accès à de nouvelles pipéridines et pyrrolidines polyhydroxylées". Paris 6, 2011. http://www.theses.fr/2011PA066748.

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Les glycosidases sont impliqués dans un large éventail de processus biologiques. La quête d'inhibiteurs puissants et sélectifs de cette classe d'enzymes fait l'objet d'un intérêt croissant de la part des chimistes et des biochimistes. Un certain nombre d'iminosucres ont déjà démontré un fort potentiel thérapeutique vis à vis de plusieurs pathologies. L'objectif principal de cette thèse a été d'explorer une nouvelle voie d'accés aux homoiminosucres à cinq et six chainons, une famille d'inhibiteurs de glycosidases très prometteuse, en utilisant une stratégie basée sur l'isomérisation de cycle d'azépanes polyhydroxylés. Cette méthodologie a été appliquée à plusieurs iminosucres à sept chainons pour fournir les pyrrolidines ou les pipéridines polyhydroxylées correspondantes, certaines présentant des motifs acétamide ou fluorés inédits. Ce réarrangement du squelette carboné a aussi permis de générer de nouveaux iminosucres bicycliques. Enfin des azépanes portant une fonction oxazoline ont aussi été préparés afin d'interagir avec la O-GlcNAcase, une glycosidase d'intérêt thérapeutique.
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41

Drzymala, Luke. "Phosphorylation of human salivary proline-rich proteins in cultured cells". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0001/MQ40692.pdf.

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42

Soufi, Abdenour. "Oligomerisation and phosphorylation of the proline-rich homeodomain protein (PRH)". Thesis, University of Bristol, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.432944.

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43

Debrauwer, Laurent. "Etude de l'interaction glucose-proline dans la réaction de Maillard". Aix-Marseille 3, 1989. http://www.theses.fr/1989AIX30038.

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L'interaction entre le glucose et la proline dans la reaction de maillard a ete etudiee. Le compose d'amadori fructose-proline, intermediaire principal de la reaction, a ete prepare et une etude cinetique par c. L. H. P. Effectuee. La simulation et l'optimisation sur ordinateur par des methodes numeriques et analytiques permettent de proposer un mecanisme reactionnel en trois etapes. La degradation thermique du compose d'amadori fructose-proline a ete examinee au moyen du couplage c. P. G. /s. M. Et des banques de spectres
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44

He, Jing. "Design and Study of Novel Antimicrobial Peptides with Proline Substitution". Ohio University / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1257779581.

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45

Zhang, Chun-sheng. "Regulation of proline biosynthesis in plants subjected to osmotic stress /". The Ohio State University, 1997. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487948807588165.

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46

Pruitt, Corunda T. "Effects of compatible solutes on cold tolerance of propionibacterium freudenreichii and the significance of propionibacterium cold tolerance in Swiss cheese manufacturing". Connect to resource, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1123184280.

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Thesis (Ph. D.)--Ohio State University, 2005.
Title from first page of PDF file. Document formatted into pages; contains xiv, 107 p.; also includes graphics. Includes bibliographical references (p. 90-97). Available online via OhioLINK's ETD Center
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47

Flores, Ortega Alejandra. "Conformational properties of constrained proline analogues and their application in nanobiology". Doctoral thesis, Universitat Politècnica de Catalunya, 2009. http://hdl.handle.net/10803/6477.

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This thesis is composed of two parts:

We have used different computer simulation techniques to investigate the impact of different chemical modifications on the conformational preferences of proline and to examine the application of conformationally constrained proline analogues in Nanobiology.

Specifically, the first part shows the conformational study of proline derivatives that were obtained by introducing one or more double bonds in the pyrrolidine ring, by replacing the α-hydrogen atom by an alkyl group or by incorporating a polar substituent at the β- or γ-position of the pyrrolidine ring. These conformational investigations were performed using Quantum Mechanical calculations at the DFT (Density Functional Theory) levels. Furthermore, the influence of the solvent on the preferences of the different proline derivatives was examined using the Polarizable Continuum Model (PCM).

The second part of the work consists on the design of a constrained proline derive able to protect a tumor-homing peptide from the attack of proteases but retaining, or even enhancing, its intrinsic biological activity. For this purpose, the bioactive conformation of the tumor-homing peptide was determined and characterized using a computational strategy based on the combination of Simulated Annealing combined with Molecular Dynamics. After this, the designed proline was derivative was introduced in the biological peptide using a targeted replacement strategy. The efficiency of the synthetic derivative was examined in silico using classical force-field simulations.
La presente Tesis consta de dos partes:

Se han empleado distintas técnicas de simulación computacional para investigar el efecto de diferentes modificaciones químicas en las preferencias conformacionales de las prolina, así como para examinar la aplicación de análogos de prolina conformacionalmente restringidos en Nanobiología.

Concretamente, la primera parte presenta el estudio conformacional de los derivados de la prolina que se obtienen al introducir uno o más enlaces dobles en el anillo de pirrolidona, al substituir el hidrógeno α por un grupo alquilo o al incorporar un grupo polar en la posición β ó γ del anillo de pirrolidona. Estos estudios conformacionales se desarrollaron mediante métodos mecano-cuánticos basados en la Teoría del Funcional de la Densidad (DFT). Además, el efecto del disolvente en las preferencias de los diferentes derivados de prolina se investigo empleado el método PCM (Polarizable Continuum Model).

La segunda parte del trabajo consistió en el diseño de un análogo de prolina capaz de proteger un péptido que actúa como marcador tumoral del ataque de las proteasas a la vez que retiene, o incluso mejora, su actividad biológica. Para conseguir esto, inicialmente se determinó y caracterizó la conformación bioactiva del péptido empleando una estrategia computacional basada en la combinación de templado simulado y Dinámica Molecular. A continuación el derivado de prolina diseñado se incorporó en una posición específica del péptido y la eficiencia del sistema resultante fue estudiada in silico usando simulaciones basadas en potenciales clásicos.
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48

Chan, Maggie Tin Lai. "Proteolytic processing of recombinant human salivary proline-rich protein precursors (PRPs)". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0025/MQ50333.pdf.

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49

Kofler, Michael. "GYF domains a class of proline rich ligand binding adaptor domains /". kostenfrei, 2007. http://www.diss.fu-berlin.de/2007/261/index.html.

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50

Tempel, Wolfram. "Synthesis and in vitro activity of proline-based ketomethylene dipeptide isoteres". Thesis, University of Salford, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.299103.

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