Rozprawy doktorskie na temat „BRET”
Kliknij ten link, aby zobaczyć inne rodzaje publikacji na ten temat: BRET.
Utwórz poprawne odniesienie w stylach APA, MLA, Chicago, Harvard i wielu innych
Sprawdź 50 najlepszych rozpraw doktorskich naukowych na temat „BRET”.
Przycisk „Dodaj do bibliografii” jest dostępny obok każdej pracy w bibliografii. Użyj go – a my automatycznie utworzymy odniesienie bibliograficzne do wybranej pracy w stylu cytowania, którego potrzebujesz: APA, MLA, Harvard, Chicago, Vancouver itp.
Możesz również pobrać pełny tekst publikacji naukowej w formacie „.pdf” i przeczytać adnotację do pracy online, jeśli odpowiednie parametry są dostępne w metadanych.
Przeglądaj rozprawy doktorskie z różnych dziedzin i twórz odpowiednie bibliografie.
1
Eustace, Natalie Margaret. "Biological Realistic Education Technology (BRET)". Thesis, University of Canterbury. HIT Lab NZ, 2014. http://hdl.handle.net/10092/9242.
Pełny tekst źródłaStreszczenie:
The aim of this project was to develop and evaluate an interactive Augmented Reality interface for teaching children aged 8 to 15 about biological systems present in the human body. The interface was de- signed as one component of a “human body scanner” exhibit, which is to be featured at the ScienceAlive! Science Centre. In the exhibit, the interface allows visualization and interaction with the body systems while being moved across a human male mannequin named BRET.
Prior research has shown that Augmented Reality, Visualization applications, and games are viable methods to teach biology to university aged users, and Augmented Reality and interactive systems have been used with children and learning biology as well.
BRET went through three iteration phases, in the first phase, prototypes were evaluated by ScienceAlive! and designs and interactions were implemented, while the use of Augmented Reality through a transparent display was rejected. Iteration two included integration of the non-transparent touch display screen and observational evaluation of six children from 9 to 15 years old. This evaluation resulted in design and interaction changes. Iteration three was the last iteration where final interface and interaction modifications were made and re- search was conducted with 48 children from the ages 8 to 15. This was to determine whether learning, fun, and retention rates were higher for children who interacted with BRET versus those who watched video clips, or read text. Each child used one learning method to learn the three different body systems: skeletal, circulatory, and digestion. The results of the final evaluation showed that overall there was no significant difference in the children’s rating of fun or the amount of information they retained between the different learning methods. There was a positive significant difference between some of the expected fun scores and the actual fun scores. It was also found that learning with text was higher than the interactive condition but there was no differences between learning with video and interaction, or with text and video.
2
Borghei, Golnaz. "Design of a BRET fluorescent protein". Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607666.
Pełny tekst źródła
3
Young, Joshua B. "Bret & Vince Get Framed for Murder". Ohio University Honors Tutorial College / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1339258933.
Pełny tekst źródła
4
Weissenberg, Clare. "This is not an exit : reading Bret Easton Ellis". Thesis, University of Essex, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.361020.
Pełny tekst źródła
5
Nystrand, Alexander. "Patrick Bateman, Violence and Consumption: Bret Easton Ellis’s American Psycho". Thesis, Södertörns högskola, Institutionen för kultur och kommunikation, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-7875.
Pełny tekst źródłaStreszczenie:
This essay investigates how Bret Easton Ellis portrays Patrick Bateman as a projection of American society, in order to criticize consumerism and capitalism in his novel American Psycho. By applying Marxist theory, this essay examines Bateman's consumption patterns and class-consciousness using key Marxist terms. This essay investigates the relationship between Bateman and his commodities, through the Marxist concept of value. Furthermore, this essay suggests that Bateman's consumption pattern creates his identity and that Bateman's lust for consumption has no boundaries. Bateman quenches his thirst for consumption by consuming humans of low status on the social hierarchy, by acts of violence, rape or cannibalism.
6
Silva, Luciano Cabral da. "The fourfold serial killer in Bret Easton Elliss American Psycho". Universidade do Estado do Rio de Janeiro, 2015. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=8749.
Pełny tekst źródłaStreszczenie:
Conselho Nacional de Desenvolvimento Científico e TecnológicoPatrick Bateman, o protagonista narrador do romance American Psycho (1991), de Bret Easton Ellis, confunde por ser rico, bonito e educado e, ao mesmo tempo, torturador, assassino e canibal. Mas esta personalidade antagônica não o torna singular. O que o particulariza são as quatro faces que ele apresenta ao longo de sua narrativa: (1) ele consome mercadorias e humanos, (2) compete para ter reconhecimento, (3) provoca horror por suas ações, e (4) não é um narrador confiável. Sendo um yuppie (termo popular usado nos Estados Unidos na década de 1980 para denominar jovens e bem sucedidos profissionais urbanos), Bateman é materialista e hedonista. Ele está imerso em uma sociedade de consumo, fato que o impossibilita de perceber diferenças entre produtos e pessoas. Sendo um narcisista, ele se torna um competidor em busca de admiração. No entanto, Bateman também é um serial killer e suas descrições detalhadas de torturas e assassinatos horrorizam. Por fim, nós leitores duvidamos de sua narrativa ao notarmos inconsistências e ambiguidades. Zygmunt Bauman (2009) afirma que uma sociedade extremamente capitalista transforma tudo que nela existe em algo consumível. Christopher Lasch (1991) afirma que o lendário Narciso deu lugar a um novo, controverso, dependente e menos confiante. A maioria das vítimas de Bateman são membros de grupos socialmente marginalizados, como mendigos, homossexuais, imigrantes e prostitutas, o que o torna uma identidade predatória, segundo Arjun Appadurai (2006). A voz autodiegética e a narrativa incongruente do protagonista, contudo, impedem que confiemos em suas palavras. Estas são as quatro faces que pretendo apresentar deste serial killer
The autodiegetic protagonist Patrick Bateman, in Bret Easton Elliss American Psycho (1991), is a troubling character, for he is highly-educated, wealthy and handsome as well as a torturer, a killer and a cannibal. This antagonistic behavior, nonetheless, does not make him a singular character. The four sides he presents throughout the novel are singular, though: (1) he consumes humans and commodities equally; (2) he competes for recognition and admiration; (3) his acts are horrific; and (4) his narration is unreliable. As a yuppie (a popular term from the 1980s used to define young urban U.S. professionals), Bateman is materialistic and hedonistic. As he lives off the excesses of a consumer society, he is incapable of distinguishing people from products. As a self-absorbed, narcissistic protagonist, he becomes a competitor struggling to get approval from his peers. Nevertheless, Bateman is a serial killer, and his detailed descriptions of tortures and murders are horrifying. Finally, we readers cannot rely on his narrative once we notice ambiguities and divergences. Zygmunt Bauman (2009) posits that an extremely capitalist society forces people to be commodified. Christopher Lasch (1991) asseverates that the old legendary Narcissus gave birth to a new one, paradoxical, dependent and less confident. Most of Batemans victims are socially-marginalized characters, members of minority groups, such as homeless people, homosexuals, immigrants, and prostitutes. As a matter of fact, Bateman may be regarded as having a predatory identity, as defined by Arjun Appadurai (2006). However, this autodiegetic narrator, together with his inconsistent narrative, cannot be entirely trusted. These are the points I want to debate regarding this fourfold serial killer
7
Helm, Kimberly Anne. "Is everything disposable? Bret Easton Ellis, abortion, and consumer culture". [Gainesville, Fla.] : University of Florida, 2004. http://purl.fcla.edu/fcla/etd/UFE0004643.
Pełny tekst źródła
8
Issafras, Hassan. "Étude de l'oligomérisation du récepteur CCR5 par la technique de BRET". Paris 7, 2002. http://www.theses.fr/2002PA077211.
Pełny tekst źródła
9
Lacaze, Annie. "Hysteroplastie selon Bret-Palmer : à propos de 43 cas d'utérus cloisonnés". Université Louis Pasteur (Strasbourg) (1971-2008), 1986. http://www.theses.fr/1986STR1M059.
Pełny tekst źródła
10
Armando, Sylvain. "Structure quaternaire des récepteurs de chimiokines CXCR4 et CCR2 et interaction avec leur effecteurs". Thesis, Montpellier 2, 2010. http://www.theses.fr/2010MON20208/document.
Pełny tekst źródłaStreszczenie:
Les récepteurs couplés aux protéines G (RCPG) sont la famille de récepteurs membranaires la plus représentée chez les vertébrés, et la plus grande cible thérapeutique chez l'Homme. L'évolution du paradigme initial qui énonçait une stœchiométrie récepteur : protéine G : effecteur de 1 :1 :1 sera présentée sur le modèle des récepteurs aux chimiokines CXCR4 et CCR2. Grâce à la technique de transfert d'énergie par bioluminescence (BRET), les travaux réalisés durant cette thèse montrent (1) que c'est par un couplage alternatif de CXCR4 à Gα13 au lieu de la voie classique Gαi que les cellules de cancer du sein migrent pour former des métastases, (2) que la désensibilisation de CXCR4 implique le recrutement d'une combinaison définie de protéines (GRK et arrestines) permettant l'arrêt sélectif des multiples voies engagées en réponse à l'agoniste, et (3) que le protomère CXCR4 a un rôle déterminant dans l'engagement de la protéine Gαi et le recrutement de la β-arrestine par l'hétéro-oligomère CXCR4/CCR2 lorsque CCR2 est activé. Dans cette dernière et principale étude, les résultats montrent également que le dimère CCR2 peut s' assembler au dimère CXCR4 pour former un tétramère, et que l'activation de CCR2 influence la conformation du dimère CXCR4. Les phénomènes de coopérativité et d'activation asymétrique déjà rapportés pour cet hétérodimère pourraient donc impliquer l'interaction de quatre protomères. En conclusion les travaux effectués durant cette thèse démontrent une régulation supplémentaire de l'activité des récepteurs chimiokines au niveau de leur structure quaternaire, de leur signalisation, et de l'arrêt de cette signalisationG protein coupled receptors (GPCR) are the most represented cell surface receptors among vertebrates, and the major therapeutic target in humans. The initial paradigm stating a 1 :1 :1 stoichiometry for receptor :G protein :effector has evolved to a more complex model, as illustrated here with the example of the chemokine receptors CXCR4 and CCR2. Bioluminescence resonance energy transfer (BRET) was used to demonstrate that (1) CXCR4 is able to couple Gα13 instead of Gαi to promote breast cancer metastasis, (2) the multiple pathways engaged by stimulation of CXCR4 are selectively desensitized by the specific recruitment of a defined combination of proteins (GRKs and arrestins) and (3) the CXCR4 protomer plays a crucial role during Gαi engagement and β-arrestin recruitment by the CXCR4/CCR2 heterodimer upon CCR2 activation. In this last and main study, the results shown also demonstrate that CCR2 dimers could assemble with CX CR4 dimers into hetero-tetramers, and that CCR2 activation leads to a conformational change in the CXCR4 dimer. Former results showing cooperativity and asymmetric activation of a simple CXCR4/CCR2 heterodimer could then be applied to a tetramer. To conclude, the work done during this thesis demonstrates a more sophisticated regulation of chemokine receptors than previously suspected at 3 different levels: quaternary structure of the protomers, G protein signalling, and signalling termination
11
von, Seth Oscar. "Psykopaten i garderoben : En queer läsning av Bret Easton Ellis American Psycho". Thesis, Södertörns högskola, Institutionen för kultur och lärande, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-21456.
Pełny tekst źródłaStreszczenie:
The novel American Psycho was first published in 1991. It recieved harsh criticism and was viewed as a work of heterosexism, misogyny and pointless violence. Despite the criticism, the protagonist, a wealthy serial killer yuppie namned Patrick Bateman, fascinated the readers. He hides his monstrosity behind a façade of heteronormativity, but this essay shows that the norms in American Psycho are fragile. Batemans relationships are shallow, his identity is constructed out of traditional masculinitynorms and even though he’s homophobic there’s a homoerotic undertone in the text, as well as gothic patterns that give the novel a fair amount of queerness too. This analysis shows that the fear of AIDS, imprinted in the text, works as a representation for Bateman’s discrepancy concerning his sexuality. It brings to light that Bateman’s feelings towards two of his collegues are charachterized by homoerotic yearnings, and that shallow readings, where the text is not interpreted, allows the brutal violence to divert attention from the novel’s queer meaning.Romanen American Psycho publicerades 1991. Den fick hård kritik och sågs som ett heterosexistiskt, misogynt verk fullt av meningslöst våld. Trots kritiken fascinerade protagonisten, den förmögna seriemördaryuppien Patrick Bateman, läsarna. Bateman döljer sin monstrositet bakom en heteronormativ fasad men den här uppsatsen visar att textens heteronorm är bräcklig. Batemans relationer är ytliga, identiteten är konstruerad från traditionella maskulinitetsnormer, han är homofobisk, även då gotiska, homoerotiska undertoner präglar texten. Analysen visar att AIDS-skräcken som präglar boken är synonym med Batemans sexualitetsdiskrepans, att hans känslor för två av hans kollegor är av homoerotisk karaktär, samt att i ytliga läsningar av romanen, där texten inte tolkas, gör det explicita våldet att läsarens uppmärksamhet avleds från romanens queera innebörd.
12
Mortimer, Danielle. "Traumatic seductions : Bret Easton Ellis's Lunar park, the postmodern, and the reader". Thesis, University of Essex, 2011. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.549291.
Pełny tekst źródła
13
RHONE, GERARD PASCALE. "Resultats obstetricaux apres intervention de bret-palmer : a propos de 27 observations". Reims, 1988. http://www.theses.fr/1988REIMM033.
Pełny tekst źródła
14
Onfroy, Lauriane. "Développement de biosenseurs BRET prédictifs de la cardiotoxicité des médicaments anti-tumoraux". Thesis, Toulouse 3, 2016. http://www.theses.fr/2016TOU30071/document.
Pełny tekst źródłaStreszczenie:
Beaucoup de médicaments anticancéreux entrainent à plus ou moins long terme une cardiotoxicité, dont les mécanismes moléculaires restent encore très mal connus. Aujourd'hui, peu de moyens existent pour prédire, au stade du développement préclinique, le potentiel cardiotoxique de ces molécules. Dans ce contexte, l'objectif de ce travail de thèse a consisté à utiliser le principe de transfert d'énergie bioluminescente par résonance (BRET) pour créer des biosenseurs de l'activité des phosphoinositide-3-kinases (PI3K) de classe IB. En effet, ces protéines kinases ubiquitaires sont des cibles pharmacologiques majeures de part leur implication dans les phénomènes cancéreux, et sont également importantes pour l'homéostasie cardiaque. Ainsi, elles pourraient constituer une cible cardiaque des agents anticancéreux participant au développement de la cardiotoxicité. Les PI3K-IB sont des hétérodimères formés d'une sous-unité catalytique (C) p110y associée à une sous-unité régulatrice (R) dont il existe deux isoformes, p87 et p101. Pour développer le senseur PI3Ky, un donneur (Rluc8) et un accepteur (GFP2) d'énergie BRET ont été fusionnés en position N- ou C-terminale de chacune des sous-unités C et R (senseur intermoléculaire). Après vérification de l'expression cellulaire de ces constructions dans les cellules HEK293T, les interactions entre les différentes paires de senseurs BRET (8 combinaisons au total) C et R ont été mesurées, en temps réel dans les cellules vivantes, à la recherche d'un couple capable de refléter l'activation de la PI3K. Nos résultats démontrent des interactions spécifiques basales entre les sous-unités C et R des couples p110y-p87 et p110y-p101. Par contre, aucune des conditions de stimulations testées (nature du récepteur, concentrations de ligand, temps de stimulation, stœchiométrie des senseurs) n'a permis de détecter des modulations du signal BRET. La présence de co-activateurs connus de la PI3Ky tels que les protéines Ras ou les protéines G hétérotrimériques n'a pas non plus aidé à la modulation du signal BRET. Nous avons ensuite tenté de créer un senseur indirect de l'activité de la PI3Ky en mesurant par BRET les interactions entre les sous-unités de PI3K et des protéines G (décrites dans la littérature) lors de l'activation de la PI3Ky. Étonnamment, les résultats révèlent une pré-association basale entre les sous-unités de la PI3Ky et celles des protéines G mais sans détection de modulation significative de signal BRET après stimulation. En conclusion, nous n'avons pu établir un biosenseur BRET de l'activité de la PI3Ky en mesurant les interactions entre les sous-unités régulatrices et catalytiques. Ainsi, l'absence de modulations détectables du signal BRET après stimulation entre les deux sous-unités pré-complexées pourrait rendre compte d'un mécanisme d'activation impliquant des changements conformationnels plutôt qu'une association-dissociation physique. Dans le futur, l'étiquetage intramoléculaire des sous-unités C ou R avec les deux sondes BRET pourrait peut-être mieux détecter ces conformations et l'activité de la PI3Ky. D'un point de vue fondamental, l'existence de pré-complexes PI3Ky-protéines G pourraient également rendre compte d'une régulation spatio-temporelle plus fine et spécifique de la kinase, en accord avec les récents concepts de modules de signalisation préformésCardiotoxicity is a recognized long-term consequence of a lot of anticancer drugs, but its mechanisms are not well-known. To date, the cardiotoxic potential of anticancer drugs is difficult to predict during preclinical studies due to the lack of appropriate tools. In this context, the aim of this thesis project was to develop biosensors, based on the use of bioluminescent resonance energy transfer (BRET) principle, to measure class IB phosphoinositide-3-kinases (PI3K) activity. Indeed, these ubiquitous kinases are major pharmacological oncotargets due to their participation in cancer development, but they also have a preponderant role in cardiac homeostasis. Thus, they could represent cardiac targets for anticancer drugs participating in the development of cardiotoxicity.PI3K-IB are heterodimers of a catalytic subunit (C) p110y and a regulatory subunit (R) p87 or p101. To develop the PI3Ky sensor, BRET energy donor (Rluc8) and acceptor (GFP2) were fused at the N-terminal or C-terminal of each subunit (intermolecular sensor). After expression control of each probe in HEK293T cells, the interactions between C and R subunits for all BRET pairs (8 combinations) were studied, in living cells in real time, so to find one able to sense PI3Ky activation. Our results indicated specific basal interaction between C and R subunits from p110y-p87 and p110y-p101 pairs. However, none of the stimulation conditions tested (receptor nature, ligand concentration, stimulation kinetics and biosensor stoichiometry) allowed the detection of BRET signal modulation. Further addition of p110y co-activators such as Ras proteins or heterotrimeric G proteins, did not improve BRET modulation. We then tried to create an indirect BRET sensor of PI3Ky activity by measuring the interaction between PI3K and G proteins subunits, known to occur during PI3Ky activation. Surprisingly, results highlighted a basal pre-association of PI3Ky and G protein subunits without BRET modulations upon PI3Ky stimulation.In conclusion, we failed to create a BRET biosensor of the PI3Ky activity based on the measure of the interaction between the catalytic and regulatory subunits. However, the pre-association of PI3K subunits without BRET modulation could reflect a mechanism of PI3Ky activation based on conformationals changes of the complex between C and R subunits rather than physical association-dissociation. In the future, intramolecular labeling of the C or R subunit with the two BRET probes could better detect conformational changes and therefore PI3Ky activity. From a fundamental standpoint, the existence of PI3Ky-G proteins pre-complexes could reflect a specific spatio-temporal fine-tuning of the PI3K activity, in agreement with recent concept of preformed cell signaling platforms
15
Roche, David. "L'imagination malsaine : Russell Banks, Raymond Carver, David Cronenberg, Bret Easton Ellis, David Lynch /". Paris : l'Harmattan, 2008. http://catalogue.bnf.fr/ark:/12148/cb412407868.
Pełny tekst źródła
16
Zhou, Yi Yuan. "Structural Determinants of 5-Ht1a Receptor Interaction With Gαi Subunits". Thesis, Université d'Ottawa / University of Ottawa, 2011. http://hdl.handle.net/10393/19761.
Pełny tekst źródłaStreszczenie:
The 5-hydroxytryptamine (5-HT) system modulates numerous physiological and behavioural processes, and dysfunction within this system underlies many behavioural disorders, such as major depression. The 5-HT1A receptor is the primary somatodendritic autoreceptor that controls the firing rate of 5-HT neurons, but is also coupled to numerous signalling pathways. An understanding of 5-HT1A receptor signalling may lead to the development of antidepressant drugs that selectively target therapeutic pathways in treating depression. The 5-HT1A receptor is coupled to inhibitory G-proteins via its intracellular loops 2 and 3. Point mutations within these loops selectively uncouple receptor signalling pathways. In this thesis, I addressed whether mutant receptors’ uncoupling from signalling pathways is associated with alteration in G-protein interaction and coupling. Using bioluminescence resonance energy transfer (BRET) to monitor receptor-G-protein interactions, we show that both wild-type and mutant receptors demonstrate a saturable interaction with Gαi protein in unstimulated conditions. Addition of 5-HT increased the BRET signal for the wild-type 5-HT1A receptor, and this increase was blocked by a 5-HT1A receptor antagonist and G-protein blocker (pertussis toxin). Mutant receptors that were deficient in Gαi signalling, but not those that still signalled to Gαi, failed to respond to receptor activation with increased receptor-Gαi interaction. Pull down studies verified the basal and agonist-induced interaction of 5-HT1A receptors with Gαi proteins. In conclusion, we have shown that the 5-HT1A receptor interacts with Gαi consistent with a pre-coupled model and that 5-HT-induced activation enhances this interaction and requires specific residues in the intracellular loops.
17
Ettler, Justine. "The Best Ellis For Business: A Re-Examination Of The Mass Media Feminist Critique Of American Psycho". Thesis, The University of Sydney, 2013. http://hdl.handle.net/2123/10020.
Pełny tekst źródłaStreszczenie:
The Best Ellis For Business analyses the mass media feminist critique of Bret Easton Ellis’s third novel, American Psycho (1991), and employs this to challenge the dominant modes of reading Ellis’s work. The thesis identifies the major shifts in literary criticism about American Psycho, both journalistic and scholarly, and discusses them in relation to the novel’s problematic sexualisation of misogynistic violence. In particular, the neutralisation of the mass media feminist critique in scholarly literary criticism is questioned, then contextualised in terms of the backlash, and finally linked to postmodern defences of the novel that ignore the important role played by the reader. The thesis employs a mixture of narratological and theoretical approaches to perform close readings of the sexually violent scenes. The thesis challenges dominant defences of American Psycho such as the ubiquitous defence of the novel as a satire, as well as the equally prevalent defence of the novel as a postmodern classic. The formalist qualities of the novel, which this thesis claims make it a postmodern parody, prevent the novel from ever being read as a straightforward satire. Further, analyses that focus on the novel’s form at the expense of its content tend to fail to account for the reader’s response to the sexualised violence. This thesis raises the oft-ignored but important issue of reader competence, particularly in relation to the marketing practices of Ellis’s corporate publishers. It will also be argued here that the novel’s excessive ambiguity leaves the reader no choice other than to resort to their biographical knowledge of the author in order to make sense of it. Thus, the thesis rereads the novel in relation to Ellis’s biography, as well as in relation to Ellis’s recent revelations about his sexuality and his interview practice.
18
Lundberg, Robin. "Unreliable narration in Bret Easton Ellis's American Psycho and Jeff Lindsay's Darkly Dreaming Dexter". Thesis, Karlstads universitet, Institutionen för språk, litteratur och interkultur, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-34929.
Pełny tekst źródłaStreszczenie:
This essay focuses on the character Patrick Bateman in American Psycho by Bret Easton Ellis and his unreliability as a narrator and compares it to the unreliable narration of the character Dexter Morgan in Darkly Dreaming Dexter by Jeff Lindsay. These characters' respective unreliability is analyzed from the perspective of six types of unreliability suggested by James Phelan and Mary Patricia Martin: misreporting, misreading, misregarding, underreporting, underreading and underregarding. The result of the analysis is that while Patrick shows proof of being an unreliable narrator with respect to each one of the six types except underreporting and underregarding, Dexter can be connected to three of them (misreading, underreading and underregarding). Even if this might seem like an insignificant difference, the amount and the clarity in the examples of unreliability adhering to Patrick suggests that he is a much more unreliable narrator than Dexter is. This result indicates that characters can be at opposing ends of a spectrum of unreliability, on which Patrick according to this analysis is placed at the highly unreliable end of the spectrum and Dexter somewhere at the low end.Denna uppsats fokuserar på karaktären Patrick Bateman i American Psycho skriven av Bret Easton Ellis, med tanke på denna karaktärs opålitlighet som berättare. Detta jämförs med karaktären Dexter Morgan från Darkly Dreaming Dexter skriven av Jeff Lindsay och denna karaktärs opålitlighet som berättare. Detta opålitliga berättande analyseras utifrån en modell som består av sex kategorier vilka James Phelan och Mary Patricia Martin har formulerat. Dessa kategorier kallas: ”misreporting”, ”misreading”, ”misregarding”, ”underreporting”, ”underreading” och ”underregarding”. Resultatet av analysen visar på att Patricks berättande kan placeras in i fyra av dessa kategorier (”misreporting”, ”misreading”, ”misregarding” och ”underreading”). Detta i jämförelse med Dexters berättande som kan placeras in i tre av dem (”misreading”, ”underreading” och ”underregarding”). Även fast denna skillnad kan verka obetydlig är det ändå så att de exampel på opålitlighet som Patrick visar upp står att finna i fler och tydligare exempel än hos Dexter vilket innebär att Patrick kan ses som en mer opålitlig berättare än Dexter. Resultatet av analysen indikerar att olika karaktärers berättande kan återfinnas i olika ändar av ett opålitlighetsspektrum. På detta spektrum kan Patrick då placeras in som en mer opålitlig berättare än Dexter som hamnar i den mer pålitliga delen av spektrumet.
19
Andersson, Jim. "Psykopatfabriken : Maskulinitetskonstruktioner i Iain Banks The Wasp Factory och Bret Easton Ellis American Psycho". Thesis, Uppsala universitet, Litteraturvetenskapliga institutionen, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-302290.
Pełny tekst źródła
20
Ruigrok, Hermanus. "Étude en temps réel des effets cellulaires et moléculaires des champs électromagnétiques radiofréquence environnementaux". Thesis, Bordeaux, 2017. http://www.theses.fr/2017BORD0674/document.
Pełny tekst źródłaStreszczenie:
Durant les quinze dernières années, une attention particulière a été portée aux effets potentiels sur le vivant des champs radiofréquence (RF) des communications sans fil. Malgré l’intensité des efforts de recherche sur les effets biologiques et sanitaires potentiels des RF, nos connaissances en bioélectromagnétisme n’ont pu suivre l’évolution rapide des technologies.[…] La capacité des RF à provoquer un échauffement des tissus est parfaitement caractérisée. Des recommandations et des normes ont été définies afin de protéger les populations des risques associés, sachant qu’aucun échauffement n’est provoqué par l’exposition aux dispositifs de communications sans fil en raison des très faibles niveaux correspondant. Il est donc capital de savoir si l’on peut totalement exclure que des effets non-thermiques des RF de faible niveau existent au niveau moléculaire au sein de la matière vivante. L’objectif de cette thèse est d’évaluer en temps réel et sur cellules vivantes, les effets de l'exposition aux champs radiofréquences (CW, GSM-1800, UMTS, Wi-Fi, WiMax, LTE), soit au niveau moléculaire en ciblant l’activité du canal ionique TRPV1 qui est l’un des thermorécepteurs de notre organisme, soit au niveau cellulaire en étudiant le comportement général de cellules exposées aux RF à l’aide d’une technique dite « sans marquage », l’impédancemétrie. Le suivi de l’activité du canal TRPV1 sous exposition RF a été réalisé à l’aide de la technique du transfert d’énergie en résonance de bioluminescence (BRET), une technique spectroscopique qui permet l’analyse des interactions protéines-protéines ou des changements de conformation des protéines en temps réel et sur cellules vivantes. La mise en place de cette technique a demandé la construction et la caractérisation de sondes BRET ciblant les canaux TRP ainsi que la mise au point d’un dispositif de mesure déporté des spectres de BRET à l’aide d’une fibre optique, afin de pouvoir exposer les échantillons aux champs RF. La conclusion de ce volet de la thèse est que les RF sont capables d’activer le canal TRPV1 en produisant un échauffement diélectrique, mais qu’en absence d’augmentation de la température il n’y a aucun effet des RF sur le niveau d’activité basal du canal TRPV1 ou sur l’efficacité de la Capsaïcine, un agoniste, à activer TRPV1. L’analyse du comportement global de cellules en culture sous exposition RF a été réalisée à l’aide d’un système xCELLigence modifié afin de pouvoir à la fois suivre le comportement cellulaire par impédancemétrie tout en utilisant le réseau d’électrodes des plaques de mesure pour exposer les cellules mises en culture aux RF. À l’aide de ce dispositif, nous avons pu réaliser des expositions de cellules SH-SY5Y avec un DAS de 24 W/Kg sans provoquer d’échauffement dans le milieu de culture ou dans les cellules. Aucun effet des RF sur le comportement de la lignée de neuroblastome SH-SY5Y n’a cependant pu être mis en évidence, que ce soit en absence ou en présence d’une co-stimulation par un agent chimique. La conclusion de cette étude est que dans des conditions où la température reste stable, nous n’avons pas pu mettre en évidence de modification du fonctionnement du vivant que ce soit au niveau moléculaire ou au niveau cellulaire. Les outils développés dans ce travail de thèse ouvrent, de plus, d’importantes perspectives tant dans le domaine du criblage de médicaments candidats à l’aide du BRET spectral, que pour de futures études en bioélectromagnétismeThe biological and health effects of radiofrequency (RF) electromagnetic fields (EMF) exposure have been very actively studied in the past two decades, mainly triggered by concerns about potential health effects of wireless communication systems. This physical agent is among the most common, fastest-growing environmental factors, triggering concerns in the population, as even a minor effect of EMF exposure on health could have a major public health impact. While the effects of extremely low frequency electromagnetic fields (ELF EMF) on the excitation of nerve and muscle cells have been well-characterized, the only well-described effects of radiofrequency electromagnetic fields (RF EMF) on biological systems are caused by dielectric-relaxation heating. In contrast, “nonthermal” RF EMF effects refer to other potential biological effects that are not caused by temperature elevation of living tissue or cell culture medium. The investigation of such mechanisms has been hampered by the absence of robust, reliable and repeatable effects occurring as a consequence of low-level exposures, for which temperature elevation is minimal. Moreover, no plausible mechanistic hypotheses have been given concerning thermal or nonthermal effects of low-level RF EMF exposures, making difficult to draw conclusions on the basis of available experimental results. Nonetheless, in 2011, the International Agency for Research on Cancer (IARC) classified RF emitted by cell phones as “possibly carcinogenic to humans” (Class 2B). The characterization of nonthermal biological RF EMF effects is therefore of primary importance for setting safety limits since guidelines and standards have so far been set to protect from the known health risks associated only with the thermal effects of RF EMF exposures. The aim of this basic science thesis work is to characterize the effects of environmental RF EMF signals on living matter at the cellular and molecular level. In this work, we took advantage of modern and innovative methods to observe the behavior of living matter under RF EMF exposure in real time at various specific absorption rates (SAR). In particular, we have studied: (i) Specific RF EMF effects on the ionic channel TRPV1, a major thermoreceptor in our body. TRPV1 activation under RF EMF exposure was studied using the bioluminescence resonance energy transfer (BRET) technique. The implementation of this technique called for the construction and characterization of BRET probes targeting TRP channels as well as the development of a device for the remote measurement of BRET spectra, using an optical fiber. The conclusion of this part of the thesis is that RFs are able to activate the TRPV1 channel by producing a dielectric heating but in the absence of temperature increase there is no RF effect on the basal activation state of TRPV1 and no change of capsaicin maximal efficacy to activate TRPV1. (ii) The analysis of the global behavior of cells in culture under RF exposure was carried out using a modified xCELLigence system where the array of electrodes of the measuring plates were also used to expose the cells to RF EMF. Using this device, we were able to perform SH-SY5Y cell exposures with a SAR of 24 W/kg without causing heating in the culture medium or in the cell culture. No effect of RF EMF on the behavior of the neuroblastoma SH-SY5Y line could however be demonstrated, either in the absence or in the presence of a co-stimulation by a chemical agent. The conclusion of this study is that under conditions where the temperature remains stable, we have not been able to demonstrate any changes in the functioning of living cells, ether at the molecular level or at the cellular level. The tools developed in this thesis work offer important prospects both in the field of drug screening using spectral BRET, and pave the ways for future studies in bioelectromagnetics
21
Alt, Constanze. "Zeitdiagnosen im Roman der Gegenwart Bret Easton Ellis' American Psycho, Michel Houellebecqs Elementarteilchen und die deutsche Gegenwartsliteratur". Berlin Trafo, 2009. http://d-nb.info/992353327/04.
Pełny tekst źródła
22
Weibels-Balthaus, Gregor. "The self in trouble: young adults in the urban consumer society of the 1980s in Janowitz, Ellis, and McInerney". [S.l. : s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=976449706.
Pełny tekst źródła
23
Perzo, Nicolas. "Rôle des déterminants moléculaires impliqués dans la signalisation du récepteur urotensine II". Mémoire, Université de Sherbrooke, 2013. http://hdl.handle.net/11143/6349.
Pełny tekst źródłaStreszczenie:
L’Urotensine II (UII) est un peptide cyclique de 11 acides aminés initialement isolé à partir de l’urophyse de gobie. Cette hormone est impliquée dans l'homéostasie cardiovasculaire et exerce la majorité de ses effets par l'intermédiaire du récepteur de l'urotensine (UT). Le récepteur UT est couplé préférentiellement à la protéine G hétérotrimérique G?q et les propriétés fonctionnelles de ce récepteur ont principalement été étudiées pour sa capacité à induire la production d'inositol phosphate ainsi que la mobilisation de Ca[indice supérieur 2+] intracellulaire. Il a été rapporté que UT peut également coupler à d'autres protéines G hétérotrimériques G?i/o et qu’il peut activer plusieurs voies indépendantes de la protéine G, tels que la voie des MAP Kinase et de la beta-arrestine. Notre hypothèse stipule que différents ligands d’UT peuvent induire ou stabiliser différentes conformations du récepteur, chacune conduisant à une signalisation spécifique, ce concept est connu sous le nom de sélectivité fonctionnelle ou signalisation biaisée. Nous avons sélectionné 6 analogues de UII qui diffèrent dans leur structure chimique et nous avons évalué leur capacité à activer plusieurs voies de signalisation: G?q G?i, G?13, ERK, NF?B et le recrutement de la ?-arrestine. Par ailleurs, la technologie de transfert d’énergie bioluminescente par résonnance (BRET) fut utilisée pour évaluer l’activation spécifique des protéines G?q, G?i, G?13 ainsi que le recrutement de la ?-arrestine-2. Nous avons montré que la substitution de la lysine en huitième position de UII affecte la propriété du peptide à activer certaines voies de signalisation. De plus, l’analogue Nle8-UII agit comme agoniste complet sur la voie G?q mais active faiblement le recrutement de la ?-arrestine-2 ainsi que la voie NF?B. Cette étude a permis d’identifier des ligands sélectifs pour certaines voies de signalisation d’UT et pourrait permettre la conception de ligands sélectifs pour UT dans diverses pathologies associées à ce récepteur. [symboles non conformes]
24
Nabo, João Luís Brejo. "O escritor e o seu duplo em Bret Easton Ellis: uma contribuição para a análise do processo de auto-referencialidade no gótico norte-americano contemporâneo". Master's thesis, Universidade de Évora, 2008. http://hdl.handle.net/10174/18409.
Pełny tekst źródłaStreszczenie:
A presente dissertação tem como objectivo contribuir para o estudo do fenómeno do Duplo, inserido no género Gótico Americano, na obra do escritor norte-americano contemporâneo Bret Easton Ellis, na tradição de Stevenson, Poe e Hawthorne, os primeiros a lançar os fundamentos desta temática nas suas obras. Assim, foram estudados os motivos que conduzem ao desenvolvimento do tema do Duplo nas produções ficcionais do autor seleccionado, tendo sido fundamental o seu estudo à luz das teorias psicanalíticas de Sigmund Freud e Otto Rank. Analisámos alguns dos autores anglo- americanos, que mais colaboraram para a definição deste cânone literário, e cujas obras, temáticas e técnicas narrativas influenciaram o processo criativo de Bret Easton Ellis. Por fim, utilizámos toda a nossa pesquisa para encontrar no autor em estudo a necessidade da criação da figura do Duplo nas suas produções literárias como expressão de auto-reflexão e auto-referencialidade literária, com relevo para os seus romances American Psycho (1991) e Lunar Park (2004). ABSTRACT; The present dissertation aims to contribute to the study of the motif of the Double, in American Gothic genre, in the literary work by the contemporary North-American writer Bret Easton Ellis, who has been following the tradition of Stevenson, Poe and Hawthorne, the first writers to lay the foundations of this issue in their works. Thus, we studied the reasons that lead to the development of the theme of the Double in his fictional productions in the light of psychoanalytic theories of Sigmund Freud and Otto Rank. We examined some of the authors, classic and contemporary, who contributed to the definition of the literary canon, and whose works, themes and narrative techniques influenced the creative process of Bret Easton Ellis. Finally, we used our entire research to find in the author the need to create the figure of the Double in his literary productions as an expression of self-reflexivity, self-referentiality, namely in his novels American Psycho (1991) and Lunar Park (2004).
25
Goyet, Elise. "Dynamique et fonction des interactions entre récepteurs du glutamate et de la dopamine". Thesis, Montpellier, 2017. http://www.theses.fr/2017MONTT019.
Pełny tekst źródłaStreszczenie:
Dans certaines aires cérébrales, l’action synergique du glutamate et de la dopamine est nécessaire pour induire et maintenir la plasticité synaptique. Un dialogue fonctionnel entre le récepteur métabotropique du glutamate mGlu5 et le récepteur de la dopamine D1 a été mise en évidence. Par ailleurs, de nombreuses études ont démontré que les récepteurs couplés aux protéines G ont la capacité de former des hétéromères créant ainsi de nouvelles entités fonctionnelles. En s’appuyant sur l’hypothèse d’une hétéromérisation des récepteurs, l’objectif de ce projet de thèse était d’étudier les mécanismes moléculaires qui sous-tendent une synergie fonctionnelle entre les récepteurs mGlu5 et D1. Dans la première partie de ce travail, j’ai caractérisé les bénéfices de la Nanoluciférase, une luciférase très lumineuse, pour améliorer la technique de BRET en imagerie (Bioluminescence Resonance Energy Transfer imaging) qui permet d’étudier la dynamique des interactions entre protéines dans les cellules vivantes. Les bénéfices mis en évidence en termes de résolution spatio-temporelle, de stabilité et de sensibilité du signal ont été exploités pour la suite de ce projet. Dans la seconde partie de ce travail, les améliorations techniques mentionnées ci-dessus ont permis de mettre en évidence pour la première fois des hétéromères mGlu5/D1 dans des neurones en culture. En outre, nous avons montré que la co-expression des récepteurs mGlu5 et D1 en système hétérologue favorise la signalisation calcique, d’une part en augmentant l'activité constitutive de mGlu5 et, d’autre part, en créant une voie de libération du calcium intracellulaire atypique induite par l'agoniste D1.Ces résultats apportent de nouveaux éléments de compréhension des bases moléculaires du dialogue fonctionnel glutamate/dopamine dans le contrôle de la communication neuronale en conditions physiologiques et ouvrent la voie à de nouvelles stratégies thérapeutiques capables de moduler sélectivement la fonction des hétéromèresIn some specific brain areas, synergism between glutamate and dopamine transmission is required to induce synaptic plasticity. Metabotropic glutamate receptor mGlu5 and dopamine receptor D1 are both known to control synaptic plasticity. Moreover, multiple lines of evidence converge toward the ability of G-protein coupled receptors to form dynamic heteromers thereby creating new entities with unique properties. Focusing on the hypothesis of receptor heteromerization, my PhD project aimed at investigating the molecular mechanisms underlying a functional interplay between mGlu5 and D1 receptors.To address this issue, a first part of this work consisted in improving single-cell Bioluminescent Resonance Energy Transfer (BRET) imaging, a technology enabling to study real time protein-protein interaction dynamics in living cells. Using the Nanoluciferase, an extremely bright luciferase, we characterized a faster and higher resolution single-cell BRET imaging technique with unprecedented performance in terms of temporal and spatial resolution, duration of signal stability and signal sensitivity. In the second part of this project, we showed that mGlu5 and D1 can form heteromers in heterologous expression system. The above-mentioned improvements of single-cell BRET imaging technique allowed to evidence the occurrence and the dynamics of mGlu5/D1 heteromers in cultured primary neurons. Furthermore, our results showed that the co-expression of mGlu5 and D1 receptors modifies single receptor properties to favor calcium signaling by increasing mGlu5 constitutive activity and creating a D1 agonist-induced activation of Ca2+ release from intracellular stores.These findings advance our knowledge about the molecular basis of the glutamate/dopamine functional dialogue to control neuronal communication in physiological conditions. Further investigation will help the dissection of the mGlu5/D1 heteromer specific signaling pathway with the hope of defining new therapeutics that may selectively modulate heteromer function and thus bypass undesirable side effects
26
Bengtsson, Tomas. "Självframställningens dilemma : En biografisk och tematisk undersökning av självframställningen i Bret Easton Ellis roman Lunar Park". Thesis, Uppsala universitet, Litteraturvetenskapliga institutionen, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-191833.
Pełny tekst źródła
27
Annesley, James. "Blank fiction : culture, consumption and the contemporary American novel". Thesis, University of Sussex, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321347.
Pełny tekst źródła
28
Busonik, Stephen. "Epistemic structuralism in the postmodern novel : the examples of William Gaddis, J.G. Ballard, and Bret Easton Ellis /". Connect to resource, 1993. http://rave.ohiolink.edu/etdc/view.cgi?acc%5Fnum=osu1260971951.
Pełny tekst źródła
29
FERRARI, Federica. "Pharmacological profile of nociceptin/orphanin FQ receptor – characterization of novel peptide and non peptide ligands". Doctoral thesis, Università degli studi di Ferrara, 2017. http://hdl.handle.net/11392/2478798.
Pełny tekst źródłaStreszczenie:
Il peptide nocicettina/orfanina FQ (N/OFQ) è il ligando endogeno del recettore NOP; questo sistema peptidergico controlla diverse funzioni biologiche sia nel sistema nervoso centrale che in periferia. Lo scopo del presente studio è stata la caratterizzazione farmacologica di nuovi ligandi peptidici e non-peptidici per il recettore NOP. Una serie di composti N/OFQ dimerici e l’antagonista PWT2-UFP-101 sono stati progettati e sintetizzati nella nostra Università, mentre i ligandi non-peptidici investigati provengono da case farmaceutiche. Tutti i composti sono stati valutati in vitro in diversi saggi, incluso il binding recettoriale, il binding GTPγ[35S], la mobilizzazione del calcio intracellulare in cellule esprimenti i recettori ricombinanti umani e proteine G chimeriche, il trasferimento di energia bioluminescente per risonanza (BRET) volto ad indagare l’interazione del recettore con la proteina G e con la β-arrestina 2, e studi su tessuti isolati. Una serie di ligandi dimerici NOP con spacers di lunghezze differenti, sono stati generati usando come farmacoforo il peptide N/OFQ(1-13)NH2. I composti sono stati investigati nel saggio del calcio e nel saggio del vaso deferente di topo (mVD). La dimerizzazione non ha modificato l’attività del farmacoforo peptidico e ha fatto recuperare potenza ai ligandi. Questo effetto sembra dipendere dalla presenza nei composti dimerici di un doppio ”address domain”. L’approccio “peptide welding technology” (PWT) è stato applicato all’antagonista NOP UFP-101. PWT2-UFP-101 è stato valutato nel saggio di BRET per studiare l’interazione NOP/proteina G e nel mVD. La molecola mantiene l’attività antagonista, il comportamento competitivo e la potenza di UFP-101. In vivo, PWT2-UFP-101 è stato testato sui topi nel test del nuoto forzato, dove è stato in grado di esercitare gli stessi effetti antidepressivi di UFP-101, mostrandosi 10 volte più potente. Tuttavia, PWT2-UFP-101, ha inibito l’attività locomotoria spontanea. Gli agonisti non-peptidici Ro 65-6570, Ro 2q, SCH-221510, MCOPPB, AT-403, AT-202 e SCH-486757 sono stati caratterizzati in dettaglio. Essi si sono comportati da agonisti pieni NOP mostrando il seguente ordine di potenza MCOPPB>AT-403>Ro 65-6570=Ro 2q>SCH-221510>AT-202>SCH-486757. Inoltre, tutte le molecole hanno mostrato un certo grado di bias verso la proteina G, tranne AT-403 che si è comportato come agonista non-bias. MCOPPB e AT-403 sono risultati i composti più potenti e selettivi sia sui recettori NOP umani che murini. Cinque agonisti parziali NOP AT non-peptidici sono stati caratterizzati e hanno mostrato elevata affinità per il recettore NOP comportandosi come agonisti NOP in tutti i saggi funzionali, mostrando il seguente ordine di potenza AT-127>AT-090>AT-035>AT-004=AT-001. I composti AT si sono comportati da agonisti pieni nel saggio del calcio e nel colon isolato di topo, da agonisti parziali nel saggio di GTPγ[35S] e nel saggio di BRET. È interessante notare che AT-090 e AT-127, si sono comportati da agonisti non-bias e hanno mostrato elevata selettività per il recettore NOP rispetto a Ro 65-6570 sui recettori nativi murini. Infine, l’antagonista non-peptidico AT-076 è stato caratterizzato sui recettori NOP e oppioidi nel saggio del calcio e nei saggi del vaso deferente di topo e ileo di cavia stimolati elettricamente. Sui recettori ricombinanti umani, AT-076 agisce da antagonista con il seguente ordine di potenza: kappa>>mu>>delta=NOP. La moderata potenza di AT-076 sul recettore mu e la sua bassa potenza sui recettori NOP e delta sono stati confermati nei saggi sui tessuti isolati. Questi risultati suggeriscono che AT-076 sia un antagonista kappa selettivo. In conclusione, la presente tesi ha indagato in modo dettagliato il profilo farmacologico di diversi ligandi peptidici e non-peptidici per il recettore NOP, fornendo alla comunità scientifica nuovi strumenti utili per indagare il potenziale terapeutico del recettore NOP.Nociceptin/orphanin FQ (N/OFQ) is the endogenous ligand of the N/OFQ peptide receptor (NOP) and this peptidergic system controls several biological functions in the central nervous system as well as in the periphery. The aim of the present study was the pharmacological characterization of novel peptide and non-peptide NOP ligands. A series of N/OFQ dimeric compounds and the antagonist PWT2-UFP-101 were designed and synthesized in our University while non-peptide NOP ligands were from pharmaceutical companies. All compounds were evaluated in vitro in several assays including receptor binding, stimulated GTPγ[35S] binding, calcium mobilization studies performed in cells co-expressing the human recombinant receptors and chimeric G-proteins, bioluminescence resonance energy transfer (BRET) experiments investigating receptor interaction with G protein and β-arrestin 2, and bioassay studies in isolated tissues. A series of dimeric NOP ligands with spacers of different lengths were generated using as peptide pharmacophore N/OFQ(1-13)NH2 and were pharmacologically investigated in a calcium mobilization assay and in the mouse vas deferens bioassay (mVD). Results demonstrated that dimerization did not modify the pharmacological activity of peptide pharmacophore. Moreover, when dimeric compounds were generated with short peptide pharmacophores, dimerization recovered ligand potency. This effect depends on the doubling of the C terminal address sequence in the dimeric ligand. The novel peptide antagonist PWT2-UFP-101 has been evaluated in vitro in a BRET based assay measuring NOP/G protein interaction and in mVD. The molecule maintains the antagonist activity, competitive behavior, and potency of the linear peptide. In vivo, PWT2-UFP-101 has been tested in mice in the forced swimming test where was able to elicit the same antidepressant like effects of UFP-101, being 10 fold more potent. However, PWT2-UFP-101, inhibited spontaneous locomotor activity. The non-peptide NOP agonists Ro 65-6570, Ro 2q, SCH-221510, MCOPPB, AT-403, AT-202 and SCH-486757 have been pharmacologically characterized and compared. All compounds behaved as full NOP agonists consistently showing the following rank order of potency MCOPPB>AT-403>Ro 65-6570=Ro 2q>SCH-221510>AT-202>SCH-486757. Moreover, all molecules displayed some degree of G protein biased agonism with the exception of AT-403 that behaved as an unbiased agonist. MCOPPB and AT-403 displayed the highest potency associated to the highest selectivity both at human and murine NOP receptors. A series of five AT non-peptide NOP partial agonists were also characterized. AT compounds displayed high NOP affinity and behaved as NOP agonists in all the functional assays consistently showing the following rank order of potency AT-127>AT-090>AT-035>AT-004=AT-001. AT compounds behaved as NOP full agonists in the calcium mobilization and mouse colon assays and as partial agonists in the GTPγ[35S] and BRET assays. Interestingly AT-090 and AT-127, behaved as an unbiased agonists and displayed higher NOP selectivity than Ro 65-6570 at native mouse receptors. Finally, the non-peptide antagonist AT-076 was pharmacologically characterized in vitro at NOP and opioid receptors in the calcium mobilization assay and in the electrically stimulated mouse vas deferens and guinea pig ileum. At human recombinant receptors, AT-076 acts as antagonist with the following rank order of potency: kappa>>mu>>delta=NOP. The moderate potency of AT-076 at mu receptor and its very low potency at NOP and delta receptor has been confirmed in bioassay studies. These results suggest that AT-076 should be classified as a rather selective kappa antagonist. In conclusion, the present thesis investigated in great detail the pharmacological profile of several peptide and non-peptide ligands for the NOP receptor, thus providing to the scientific community novel tools useful for investigating the therapeutic potential of the NOP receptor.
30
Hardie, Michael L. "Using Hamlet and Peter Pan: Family Issues, Ghosts, and Memory in Bret Easton Ellis's Lunar Park". ScholarWorks@UNO, 2016. http://scholarworks.uno.edu/td/2233.
Pełny tekst źródła
31
Darbandi-Tehrani, Kévin. "Etude de la structure du CX3CR1 par BRET : effet anti-tumoral de son ligand, le CX3CL1". Paris 6, 2010. http://www.theses.fr/2010PA066396.
Pełny tekst źródłaStreszczenie:
En interagissant avec leurs récepteurs couplés aux protéines G, les chimiokines, ou cytokines chimio-attractantes, induisent le recrutement spécifique des leucocytes et participent ainsi à la réponse immune et à de multiples processus homéostatiques, inflammatoires et pathologiques. En particulier, le couple adhésif formé par la chimiokine CX3CL1 et son récepteur, le CX3CR1, joue un rôle important dans, entre autres, l'athérosclérose, le glioblastome et la DMLA. Ce travail de thèse comprend deux aspects : d'une part une approche biophysique de la structure du CX3CR1 et d'autre part une étude de l'effet anti-tumoral de la molécule F2, un analogue du CX3CL1. Par la technique de BRET (Bioluminescence Resonance Energy Transfer), j'ai testé le CX3CR1 et l'effet des variations naturelles V249I et T280M. Les résultats suggèrent que tous les variants du CX3CR1 forment des oligomères et que la technique de BRET est suffisamment sensible pour détecter les différences conformationnelles entre variants. Les résultats ont été confirmés par FRET entre CX3CL1 fluorescentes. La modélisation moléculaire propose des éléments d'explication sur l'impact qu'ont les variations V249I et T280M sur les positions respectives des domaines transmembranaires I, VI et VII du CX3CR1. Par ailleurs, j'ai constaté dans un modèle murin de tumeurs sous-cutanées EG7 que l'injection intra-tumorale de protéines de fusion à base du super-agoniste F2 et d'immunoglobuline (F2-Ig) inhibait la croissance tumorale mais au même niveau que la protéine CX3CL1-Ig. Cependant, la poursuite de la caractérisation de F2 devrait permettre d'identifier des super-agonistes pouvant donner lieu à des molécules thérapeutiques
32
Langenbruch, Lisa Marie [Verfasser], i Erhard [Akademischer Betreuer] Wischmeyer. "Biolumineszenz-Resonanz-Energietransfer (BRET) zur Untersuchung der Dimerisierung des Mineralokortikoidrezeptors / Lisa Marie Langenbruch. Betreuer: Erhard Wischmeyer". Würzburg : Universitätsbibliothek der Universität Würzburg, 2011. http://d-nb.info/1014892201/34.
Pełny tekst źródła
33
d'Hont, Coco. "Brutal bodies : exploring transgression through the fiction of Chuck Palahniuk, Poppy Z. Brite, and Bret Easton Ellis". Thesis, University of East Anglia, 2016. https://ueaeprints.uea.ac.uk/59676/.
Pełny tekst źródłaStreszczenie:
This thesis explores how American transgressive fiction of the 1990s represents and interrogates transgressive processes in its extra-textual context. It shows in what ways transgressive fiction visualizes how transgression functions, not simply as a counter-cultural phenomenon, but more as a central social mechanism. The thesis makes four contributions. First, it critically assesses existing definitions of transgression as counter-cultural, instead conceptualizing transgression as a mechanism which (re)develops central social ideologies. The project traces how the transgression of ideological boundaries forms a cyclical process which (re)produces ideological frameworks. Second, the thesis uses this re-definition to explore 1990s transgressive fiction in its social context. The study investigates how the late 1980s, characterized by phenomena such as neoliberal politics and the HIV/AIDS epidemic, inspired transgressive fiction produced during the 1990s. Thirdly, the thesis constructs an interdisciplinary methodological approach to dissect how the body came to play a crucial role in this context as a site through which transgression occurred. Drawing from biopolitical and queer theory, the study deepens the understanding of transgression as both a literary phenomenon and a socio-political process. Finally, the thesis compares the work of three transgressive authors whose work has not yet been analysed together in depth. It analyses the fiction of Bret Easton Ellis and Chuck Palahniuk in combination with that of Poppy Z. Brite, an author who has, in comparison, been neglected by academia. The analysis results in an increased understanding of the dynamics of transgression in 1990s American fiction and society, showing that transgression is a cyclical process which reproduces and subsequently dissolves ideological boundaries, a practice which results in a temporary crisis which ultimately enables the (re)development of ideologies. The thesis concludes that transgressive fiction of the period represents, exaggerates and interrogates transgression as a cyclical process which (re)configures ideologies in its extra-textual context.
34
Busnelli, M. "Development of a BRET-based assay to investigate the multiple G protein coupling of the oxytocin receptor". Doctoral thesis, Università degli Studi di Milano, 2009. http://hdl.handle.net/2434/63231.
Pełny tekst źródła
35
Бойко, М. И. "Физиолого-биохимические особенности системы Pinus Sywestuis. - Heterobasidion annosur (Fr) Bret и перспективы практического использования экзометаболитов некоторых дереворазрушающих грибов." Rozprawa doktorska doktora nauk biologicznych, Донецкий гос. унив., 1996.
Znajdź pełny tekst źródła
36
Busonik, Stephen. "Epistemic structuralism in the postmodern novel : the examples of William Gaddis, J. G. Ballard, and Bret Easton Ellis". The Ohio State University, 1993. http://rave.ohiolink.edu/etdc/view?acc_num=osu1260971951.
Pełny tekst źródła
37
Busonik, Stephen William. "Epistemic structuralism in the postmodern novel: The examples of William Gaddis, J. G. Ballard, and Bret Easton Ellis /". The Ohio State University, 1993. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487847309053231.
Pełny tekst źródła
38
Ayoub, Mohammed Akli. "Etude de la dimérisation des récepteurs de la mélatonine par la technique de BRET : Bioluminescence Resonance Energy Transfer". Paris 11, 2003. http://www.theses.fr/2003PA11T043.
Pełny tekst źródła
39
Marquez, Marcel. "Optimisation de la technique de BRET : étude de l’association des variants d’HMGA1 avec le diabète de type 2". Thesis, Lille 2, 2011. http://www.theses.fr/2011LIL2S035.
Pełny tekst źródłaStreszczenie:
La technique de BRET repose sur le transfert d’énergie par résonance de type Förster qui s’opère entre une enzymeluminescente donneuse d’énergie et une protéine fluorescente acceptrice d’énergie par oxydation d’un substrat métabolisable parle donneur d’énergie. Contrairement à bon nombre de techniques d’étude des interactions protéine-protéine, le BRET est noninvasif et permet l’étude dynamique de l’interaction de deux protéines en cellules vivantes ainsi que le criblage de moléculescapables de moduler cette interaction.Dans notre étude, nous avons cherché à optimiser la technique de BRET pour le criblage moléculaire en sélectionnant lemeilleur couple de donneur/accepteur d’énergie. Un nouveau donneur d’énergie, la Rluc8, qui possède des caractéristiqueslumineuses supérieures à la Rluc, a été testé en association avec deux nouveaux accepteurs d’énergie, la YPet, un variant de laYFP optimisé pour le transfert d’énergie, et la RGFP, une protéine fluorescente issue de Renilla reniformis qui est responsableavec la Rluc du phénomène de BRET chez cet organisme marinThe BRET technique is based on resonance energy transfer has been described by Förster and is based on a luminescentdonor enzyme and a complementary acceptor fluorophore upon oxidation of a donor enzyme substrate. Unlike many techniquesfor the study of protein-protein interactions, the BRET is non-invasive and allows the dynamic study of interaction between twoproteins in living cells and the screening of molecules modulating this interaction.In our study, we sought to optimize the BRET technique for screening assay by selecting the best pair of donor / acceptor.Rluc8, a new donor, with superior light capabilities than Rluc, was tested in combination with two new acceptors, YPET, avariant of YFP optimized for energy transfer, and RGFP, a fluorophore from Renilla reniformis which is involved in theBREAT signal when in contact with Rluc. These new donors / acceptors were compared to the original Rluc / YFP combination,usually used in BRET1, in fusion proteins and in follow-up studies of protein-protein interactions involving GPCRs and βARR2in order to assess their capacity to improve the BRET technique. We were able to show that Rluc8, YPET and RGFP increasedthe sensitivity of the original BRET1 method by increasing at least 2-fold of the BRET signal. These results demonstrate theinterest of these new donors / acceptors in BRET technique and may help improving current follow-up studies of protein-proteininteractions
40
Fredriksson, Sophia. "Abandon All Hope : An Analysis of American Psycho". Thesis, Högskolan Dalarna, Engelska, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:du-6391.
Pełny tekst źródła
41
Gondoin, Anaïs. "Etude et développement d’agents insulino-sensibilisateurs inhibant l’interaction IR-Grb14". Thesis, Paris 5, 2013. http://www.theses.fr/2013PA05T018.
Pełny tekst źródła
42
Wadström, Simon. "Anteckningar från en skyskrapa : En studie av Fjodor Dostojevskijs Anteckningar från ett källarhål och Bret Easton Ellis American Psycho". Thesis, Uppsala universitet, Litteraturvetenskapliga institutionen, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-150993.
Pełny tekst źródła
43
Lutton, Alison Mary. "Authorship and the production of literary value, 1982-2012 : Bret Easton Ellis, Paul Auster, J.T. LeRoy, and Tucker Max". Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:3aa64675-73a2-42a8-be24-cb75f034e9de.
Pełny tekst źródłaStreszczenie:
Definitions of celebrity authorship and material textuality at the turn of the twenty-first century have predominantly emphasised the implicitly negative aspects of contemporary developments in the literary marketplace. Particularly prominent are arguments that the practice of authorship has become subject to homogenisation by the matrix of celebrity in which successful writers are now expected to function; and, further, that the changing nature of texts themselves and the ways in which they are marketed is eroding the implicitly superior position traditionally held by literature in the cultural marketplace. This thesis views such readings as pessimistic, and offers an alternative, seeking to formulate a new critical approach to literary value in the contemporary sphere which would appreciate notions of celebrity, populism, and digital mediation as integral and productive aspects of how literary value is formed today. Through in-depth focus on the cases of a number of unconventional contemporary American authors whose work demonstrates differing, innovative approaches to the process of authorship, this thesis exposes the ways in which contemporary, atypically ‘literary’ instances of writing can and do work within and develop beyond traditional conceptualisations of authorship and literary value. Bret Easton Ellis and Jay McInerney, largely critically considered prototypical ‘celebrity’ authors, are in the first chapter reconsidered as writers whose understanding of their position within the literary marketplace affords them a self-conscious, critical perspective on the notion of celebrity in their work and public personae. The productively self-conscious author-figure is reconsidered in the second chapter, which reads the individual and joint works of author Paul Auster and visual artist Sophie Calle as foregrounding the process of creative collaboration as uniquely illuminating and transformative within the contemporary literary sphere. The notion of dual authorship is revisited and reconceptualised in the third chapter, which considers JT LeRoy and the practice of hoax authorship, outlining how this process forces the reformulation of literary value, particularly in a contemporary setting in which authors are accountable for their work in newer, more visible ways. The final chapter expands these previously-introduced themes to consider bloggers-turned-authors, particularly Tucker Max and Julie Powell, and the impact of the merging of old and new textualities on both the orientation of the figure of the writer and the way in which value is attached to his texts by readers. Ultimately, the unconventional nature of these examples is shown to belie the universality of the representations of value they enact, contributing to a full and salient account of how literary value is determined at the beginning of the twenty-first century.
44
Leypoldt, Günter. "Casual silences : the poetics of minimal realism from Raymond Carver and the New Yorker School to Bret Easton Ellis /". Trier : Wissenschaftlicher Verlag Trier, 2001. http://catalogue.bnf.fr/ark:/12148/cb388753788.
Pełny tekst źródła
45
Simon, Alaina R. "Satire and Sympathy in American Psycho". University of Toledo Honors Theses / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=uthonors1355508133.
Pełny tekst źródła
46
Ferry, Peter. "Masculinity in Manhattan : reading hegemonic masculinity in selected novels of Paul Auster, Don DeLillo, Bret Easton Ellis and Jed Rubenfeld". Thesis, Queen's University Belfast, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.601475.
Pełny tekst źródłaStreszczenie:
The study of men and masculinities has enjoyed steady progress over the last four decades within the fields of sociology, psychology, and cultural studies. It is only in the last ten to fifteen years that Masculinity Studies scholarship has begun to recognise the sociological value of literary masculinities. An area of research still in its infancy, this thesis sets out to address the lack of critical discussion on masculinity in both the fields of Masculinity Studies and American literary studies by presenting a case-study analysis of the selected works of Paul Auster, Don Delillo, Bret Easton Ell is and Jed Rubenfeld. Gendering our reading of these Manhattan writers underscores masculinity as a central theme in their novels. What connects these authors is their employment of the one of the great figures in the history of literature: the flaneur. While scholars have identified masculinity as a major issue related to the flaneur, they have argued strongly for the disintegrative effect of the urban metropolis on the male flaneur's subjectivity. This inevitable "invisibility" of the flaneur shares notable parallels with major debates within the field of Masculinity Studies. With the majority of critical investigation placing undoubtedly worthy focus upon nonhegemonic groups of males, the hegemonic group is often overlooked and consequently becomes invisible. Carefully considering the negotiation of masculinity in these novels alongside Raewyn Connell's concept of hegemonic masculinity illustrates that the hegemonic male, rather than existing as a static character type, is a complex individual shaped in relation to the nonhegemonic other. Connell's concept allows patterns to emerge in the performance of the protagonists of these novels within the masculine-affirming hierarchical frameworks in Manhattan. Ultimately this study aims to rehabilitate the flaneur as a sociologically conscious individual employed by these authors to write their counterhegemonic narratives of masculinity in Manhattan.
47
Wagner, Annette. "Postmoderne im Adoleszenzroman der Gegenwart : Studien zu Bret Easton Ellis, Douglas Coupland, Benjamin von Stuckrad-Barre und Alexa Hennig von Lange /". Frankfurt am Main : P. Lang, 2007. http://catalogue.bnf.fr/ark:/12148/cb41147777t.
Pełny tekst źródła
48
Macha, Bret B. [Verfasser], Holger [Gutachter] Braunschweig, Todd [Gutachter] Marder i Matthias [Gutachter] Lehmann. "Boron-Containing Aromatics as Communicating and Communicative Units in π-Conjugated Systems / Bret B. Macha ; Gutachter: Holger Braunschweig, Todd Marder, Matthias Lehmann". Würzburg : Universität Würzburg, 2016. http://d-nb.info/1120924987/34.
Pełny tekst źródła
49
Hawryluk, Lynda J., University of Western Sydney, of Arts Education and Social Sciences College i of Communication Design and Media School. "Call waiting". THESIS_CAESS_CDM_Hawryluk_L.xml, 2001. http://handle.uws.edu.au:8081/1959.7/6.
Pełny tekst źródłaStreszczenie:
This thesis examines the life and career of Bret Easton Ellis, and the influences of his work on the author's development as a writer. Part one encapsulates a novel written specifically for this thesis. 'Call waiting' is a harsh look at modern friendships, the role of work in these relationships and the proliferation of shallow communication through the advent of email. A critical reflection follows, examining the process that led to the novel's creation. Three specific areas are focussed on: the direct influence of Ellis' novel 'The rules of attraction' on the overall themes of 'Call waiting', the realisation of the project and the various editing changes and narrative developments that arose during the writing of the novel, and an examination of the inspiration behind the novel's creation. Part two considers Ellis' role in the literary world of the 1980s, his own complicity in the creation of a career as a celebrity author, and the carefully manufactured persona Ellis presents to the world. In Part three the thesis is concluded with a close analysis of the publication of Ellis' controversial novel 'American psycho'. This chapter explores the negative publicity the novel attracted and the possible causes of the ensuing backlash against the author.Doctor of Philosophy (PhD)
50
Wallbanks, Mark. "The vicissitudes of the authentic self: a literary mapping of the authentic self from John Milton's Paradise lost to Bret Easton Ellis' Glamorama /Mark Wallbanks". HKBU Institutional Repository, 2017. https://repository.hkbu.edu.hk/etd_oa/364.
Pełny tekst źródłaStreszczenie:
Since the rise of individualism in the seventeenth century there has been increasing pressure on individuals to define themselves in the public eye. This has led to the recent phenomena of identity politics and self-branding. Yet how is one's true identity - if such a thing exists - ever expressed externally? How do individuals deal with the inner and outer aspects of identity? These are some of the issues which impinge upon the ethics of authenticity. This thesis investigates the development of the concept of the authentic self from its inception in the modern period to the postmodern. Through an analysis of the various tropes of literary texts, I shall illustrate how the concept of authenticity has travelled and transformed between cultural and temporal contexts. The body of the thesis contains five central chapters. Chapter 1 represents Paradise Lost (1667) as the end of one world and the beginning of another. The "Satanic" trope introduces the contingency of transgression and displacement in regard to authentic self-definition. With the birth of the modern epoch, I argue that the collapse of the epic totality instigated the liberation of self through the process of individuation, yet the corresponding loss of "place" in the social order evoked existential angst. In the second chapter I argue that Daniel Defoe's Robinson Crusoe (1719) is an apposite inclusion in the tradition of St. Augustine's and Jean-Jacques Rousseau's Confessions. Through analysis of the "island" trope I assert that, even given the most perfect conditions of solipsism, the individual remains an inherently social being that retains a primordial compulsion for dialogical inscription of the self. In chapter 3, an analysis of the trope of "voice" as a metonym for ideology in Joseph Conrad's Heart of Darkness (1902) portrays Kurtz and Marlow as opposing sides of the authenticity struggle against the ideological allure of collective and absolute power. Chapter 4 associates Henry Miller's Tropic of Cancer (1934) with the anarchic egocentrism and intense individualism of Max Stirner's philosophy as a means of rebelling against the demands of social collectivism. In this chapter I analyse the "dream" trope in terms of Miller's trademark use of surreal metaphor which, I argue, provides a means of escape from the influence of collective identities. Finally, the fifth chapter will discuss the trope of "image terrorism" in reference to Glamorama (1998). This trope addresses the problemata of the globally destabilising influences of celebrity and terrorism, the tyranny of consumerism, and the Debordian Society of the Spectacle. The chapter raises the question of how, indeed if, in a globalized postmodern world with ever reducing horizons of differentiation, travel remains the last viable option in the pursuit of the authentic self.