Rozprawy doktorskie na temat „Bordetella”
Utwórz poprawne odniesienie w stylach APA, MLA, Chicago, Harvard i wielu innych
Sprawdź 50 najlepszych rozpraw doktorskich naukowych na temat „Bordetella”.
Przycisk „Dodaj do bibliografii” jest dostępny obok każdej pracy w bibliografii. Użyj go – a my automatycznie utworzymy odniesienie bibliograficzne do wybranej pracy w stylu cytowania, którego potrzebujesz: APA, MLA, Harvard, Chicago, Vancouver itp.
Możesz również pobrać pełny tekst publikacji naukowej w formacie „.pdf” i przeczytać adnotację do pracy online, jeśli odpowiednie parametry są dostępne w metadanych.
Przeglądaj rozprawy doktorskie z różnych dziedzin i twórz odpowiednie bibliografie.
Ben, Hafsia Abdelhafidh. "Facteurs de virulence des bordetellae : le modèle de Bordetella parapertussis". Paris 11, 1991. http://www.theses.fr/1991PA114806.
Pełny tekst źródłaPhillips, Linda Jane. "Immunization against Bordetella pertussis". Thesis, Kansas State University, 1985. http://hdl.handle.net/2097/9871.
Pełny tekst źródłaSmith, Colin J. "Genetic studies with Bordetella pertussis". Thesis, University of Glasgow, 1986. http://theses.gla.ac.uk/1505/.
Pełny tekst źródłaLewandowski, Anna Zofia. "Antigenic variation in Bordetella pertussis". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ60480.pdf.
Pełny tekst źródłaSidey, Fiona M. "Metabolic effects of Bordetella pertussis". Thesis, University of Strathclyde, 1987. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=20352.
Pełny tekst źródłaLadant, Daniel. "L'adenyl cyclase de bordetella pertussis". Paris 7, 1989. http://www.theses.fr/1989PA077200.
Pełny tekst źródłaKeidel, Kristina [Verfasser], i Roy [Akademischer Betreuer] Gross. "Charakterisierung des Hfq-Regulons in Bordetella pertussis und Bordetella bronchiseptica / Kristina Keidel. Betreuer: Roy Gross". Würzburg : Universitätsbibliothek der Universität Würzburg, 2011. http://d-nb.info/1018612696/34.
Pełny tekst źródłaGOYARD, SOPHIE. "Regulation de l'adenylcyclase de bordetella pertussis". Paris 6, 1993. http://www.theses.fr/1993PA066107.
Pełny tekst źródłaAl-Turkestany, Ismail M. A. "Interaction of Bordetella bronchiseptica and different Bordetella factors with sheep bone marrow mast cells and other cell types". Thesis, University of Glasgow, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.440331.
Pełny tekst źródłaAdams, Toni Elizabeth. "Bordetella bronchiseptica dermonecrotic toxin, purification and characterisation". Thesis, University of Leicester, 1997. http://hdl.handle.net/2381/29723.
Pełny tekst źródłaCraig, Frank Furlong. "Effects of Bordetella pertussis on neutrophil leukocytes". Thesis, University of Glasgow, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.236023.
Pełny tekst źródłaIsobe, Tomoko. "Biosynthesis of inner-core LPS of Bordetella". Thesis, University of Cambridge, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.624405.
Pełny tekst źródłaCambuy, Diego Duque. "Epidemiologia genômica de Bordetella pertussis no Brasil". reponame:Repositório Institucional da FIOCRUZ, 2014. https://www.arca.fiocruz.br/handle/icict/13349.
Pełny tekst źródłaFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
A coqueluche, ou pertússis, é uma doença do trato respiratório causada principalmente pela bactéria Bordetella pertussis. Após 50 anos de vacinação, pertussis reemergiu, passando a ser a doença imunoprevinível mais frequente mesmo em países desenvolvidos. Várias são as hipóteses para a reemergência de pertússis, uma delas é a adaptação do patógeno frente à vacinação. Linhagens contemporâneas de B. pertussis diferem de linhagens do período pré-vacinal, especialmente em genes codificadores de proteínas usadas na produção de vacinas acelular. Esta re-emergência também tem sido observada no Brasil, assim, realizamos a caracterização genética por MLST baseado nesses genes, de 26 isolados B. pertussis de surtos de três regiões brasileiras (Norte, Sul e Nordeste). Foram identificados dois perfis alélicos, em 24 isolados: prn2-ptxS1A-fim3B-ptxP3, de surtos (2008-2013) de Alagoas, Pernambuco e Rio Grande do Sul - e o perfil prn2-ptxS1A-fim3A-ptxP3 , em dois isolados de Pará/2004. Análises filogenéticas agruparam esses perfis com isolados do período pós vacinal de outras partes do globo. Deste conjunto, três do perfil mais frequente e um do perfil menos frequente, tiveram seus genomas sequenciados na plataforma GS 454 Junior. A comparação desses genomas com outros genomas de B. pertussis disponíveis em dados públicos não identificou SNPs ou genes únicos que caracterizassem os isolados do Brasil Este estudo desenvolveu uma metodologia que permitiu definir a posição da IS481 nos genomas, e uma delas corresponde a um gene relacionado a regulação da transcrição da família MarR, Análise filogenômica, baseada em 826 SNPs, demonstrou que os isolados recentes do Brasil da linhagem pandêmica que presente em todos os continentes, exceto a África. Foi observado também que as relações filogenéticas inferidas pelo MLST são semelhantes àquelas inferidas quando se utiliza o genoma completo, isso denota a pressão seletiva sobre esses genes. Sendo assim, a cepa utilizada na produção da vacina no Brasil, que apresenta o perfil alélico prn1-ptxS1D - fim3A-ptxP2, pode não ser capaz de gerar uma resposta imune protetora frente às linhagens circulantes no país. Este estudo traz, pela primeira vez, informações genéticas e genômicas de isolados de B. pertussis do Brasil, país que apresenta cobertura vacinal bastante heterogênea, que utiliza, oficialmente, a vacina celular, mas que, também, aplica a vacina acelular. As informações reveladas neste estudo podem auxiliar a tomada de ações para o controle de pertússis no Brasil, além do conhecimento sobre epidemiologia e evolução de B. pertussis
Pertussis more commonly referred as whooping cough is respiratory tract disease mainly caused by the bacteria B. pertussis. After 50 years of vaccination pert ussis remerged, becoming the most frequent vaccine preventable disease in developed countries. Many hypotheses have been proposed for the re - emergence of pertussis, one being the pathogen adaptation in a vaccinated environment. Current pertussis strains ar e different than those from the prevaccination era, especially in genes that code for proteins used in acelluar pertussis vaccines. This re - emergence is also observed in Brazil, therefore we characterized 26 isolates from 3 regions of Brazil (North,South,N ortheast) using an MLST approach based on these genes. We identified two allelic profiles, 24 isolates from the states of Rio Grande do Sul (2008 - 2009), Alagoas (2008 - 2009), Pernambuco (2013) and Pará (2004) presented the prn2 - ptxS1A - fim3B - ptxP3 allelic pr ofile, while 2 isolates from Pará (2004) presented the prn2 - ptxS1A - fim3A - ptxP3 allelic profile. Phylogenetic analysis branch these two allelic profiles along with other post vaccination isolates around the globe. Four isolates, three from the dominant prof ile and one from the less frequent profile, had their genomes completed sequenced on the GS 454 Junior Platform. We compared these genomes with others available in public databases and no SNP or unique genes were identified in the Brazilian genomes. This s tudy also developed a methodology that identifies the location of the repetitive region IS481, and what genes it interrupted. One of them was the MarR transcriptional regulator gene. Phylogenomic analysis based on 826 SNPs revealed that Brazilian B. pertus sis lineages are part of the current pandemic linage present in all continents, except Africa. We also observed that phylogenomic relationships are similar to MLST’s. Therefore, strain used for pertussis vaccine in Brazil, that presents the prn1 - ptxS1D - f im3A - ptxP2 allelic profile, might not be able to induce immune response to the current linage circulating in the country. This is the first study with genetic and genomic informations of B. pertussis isolates in Brazil, which is a country with heterogeneou s vaccine coverage and mixed and has both cellular and acellular vaccine administrated to the population. Information brought with this study can help the decision making on the control of pertussis in Brazil and gives new insights on the epidemiology and evolution of B. pertussis
Colombi, Débora. "Clonagem e expressão da Região Hep do domínio de Heparina da Proteína hemaglutinina filamentosa ( FHA) da bactéria Bordella pertussis em sistemas heterólogos". Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-13112014-173027/.
Pełny tekst źródłaBordetelfa pertussis, the agent of whopping cough, establishes infection by attaching to the ciliated epithelial cells of the respiratory tract. The bacterial adherence is mediated by pertussis toxin and filamentous hemagglutinin (FHA). FHA is the major adhesin of B. pertussis and displays multipie adherence activities. FHA contains four distin\'ct domains that exhibit specific affinities for different ligands or receptor, the amino-terminal end, the RGD triplet (FHA1097-1099), the lectin domain (FHA1141-1279) and the heparin-binding domain (FHA442-863). In this study, two overlapping regions of the heparin-binding domain, Mal80 (FHA299-873) and Hep (FHA442-873), were amplified by peR and subcloned in pAE expression vectors for E. coli. The fusion proteins in pAE were transformed in E. coli BL21 SI, induced with NaCI 0,3 M and purified using a nickel-charged metal chelating resin. The purified protein has low heparin affinity and does not have hemagglutination activity. The purified protein HEP was used to produce polyclonal antibodies in mouse. The anti-HEP antibodies are able to recognize the HEP, MAL80 and FHA proteins in ELISA and western assays, but anti-FHA only recognized the FHA protein. The genetically detoxified S1 subunit of pertussis toxin and Hep domain were amplified by the TAP Express method. There are two PCR reactions involved in the TAP processo At the end of the process the fragment of interest will carry a CMV promoter and a SV40 terminator and is ready to be introduced into animals or cell by transfection. Groups were immunized with proteins and/or DNA, challenged i.c. with a lethal dose of live Bordetelfa pertussis and the survival was monitored. No groups were protected against the challenge. The recombinant protein HEP were also expressed in Lactobacilfus aiming the development of potential mucosal vaccines. The polyclonal antibodies produce in mouse immunized with DNA and protein Hep expressed in E. coli and Lactobacillus were able to inhibition the FHA hemagglutination activity.
Hegerle, Nicolas. "Evolution of Bordetella pertussis and Bordetella parapertussis under acellular Pertussis vaccine pressure : What future for whooping cough and Pertussis vaccination ?" Paris 7, 2014. http://www.theses.fr/2014PA077038.
Pełny tekst źródłaWhooping cough is an acute respiratory disease life threatening for unvaccinated young children. It is caused by Bordetella pertussis and Bordetella parapertussis, two gram-negative bacteria restricted to humans. The introduction of vaccination against B. Pertussis in the 1950s greatly changed the epidemiology of pertussis as well as the bacterial population itself. Whole cell vaccines were first introduced for children immunization and first booster and enabled to control isolates similar to strains included in the vaccines. Acellular pertussis vaccines, only targeting few bacterial antigens, were later introduced for adolescent booster vaccination before being generalized to the whole population, including adults and new-borns. In addition to a change in the type of vaccine-induced immunity, B. Pertussis also had to face increased herd immunity. Few years alter acellular pertussis vaccine introduction we demonstrated a temporal increase in the prevalence of isolates lacking the production of one vaccine antigen, pertactin, while the population remained quite monomorphic at the genetic level as compare to the post-whole cell vaccine era (allelic stability of known virulence factors). We characterized these isolates at the phenotypic level and demonstrated that they remain as virulent in different in vitro and in vivo models of host pathogen interaction while they might present a selective advantage in an acellular immunized background targeting pertactin. Although vaccines remain effective, surveillance must continue to follow the evolution of these isolate prevalence and the possible impact of pertactin loss on vaccine effectiveness
Stenson, Trevor H. "Characterization of Bvg-repressed molecules of Bordetella pertussis". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ34841.pdf.
Pełny tekst źródłaSims, Peter Vincent. "Biogenesis of BapF : a novel acylated Bordetella autotransporter". Thesis, University of British Columbia, 2012. http://hdl.handle.net/2429/41811.
Pełny tekst źródłaKing, Jerry David. "Characterisation of polysaccharide biosynthesis genes in Bordetella bronchiseptica". Thesis, University of Cambridge, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.614163.
Pełny tekst źródłaGlaser, Philippe. "Biologie moleculaire de l'adenylate cyclase de bordetella pertussis". Paris 6, 1988. http://www.theses.fr/1988PA066259.
Pełny tekst źródłaGlaser, Philippe. "Biologie moléculaire de l'adénylate cyclase de Bordetella pertussis". Grenoble 2 : ANRT, 1988. http://catalogue.bnf.fr/ark:/12148/cb376139521.
Pełny tekst źródłaNeal, Shona Elaine. "Genotypic diversity and epidemiological typing of Bordetella pertussis". Thesis, University of Glasgow, 2004. http://theses.gla.ac.uk/30856/.
Pełny tekst źródłaDill, Michael T. "Characterization of the Aspartate Transcarbamoylase that is Found in the pyrBC Complex of Bordetella Pertussis". Thesis, University of North Texas, 2001. https://digital.library.unt.edu/ark:/67531/metadc3057/.
Pełny tekst źródłaRivera-Millot, Alex. "Mécanismes de l’homéostasie du cuivre chez un pathogène strictement humain, Bordetella pertussis". Thesis, Lille 2, 2020. http://www.theses.fr/2020LIL2S009.
Pełny tekst źródłaCopper has become an essential element for most living organisms since the adventof oxygen in the Earth’s atmosphere, 2.7 billion years ago. This metal is used in manybiological processes. However, the electronic properties that make it crucial for life are alsowhat makes it poisonous. This ambivalent biological impact has forced organisms to acquirean increasing number of mechanisms dedicated to maintaining a strict control over copperhomeostasis. In addition, a few organisms such as amoebae have taken advantage of its toxicproperties. Those lower eukaryotes have developed a complex predation mechanism to feedon other unicellular organisms, phagocytosis, and make use of metal poisoning, in particularcopper, to kill their preys. Phagocytosis has persisted over the course of evolution to becomea key actor of innate immunity in metazoans, including humans. Copper detoxificationmechanisms developed by prokaryotic organisms have likely been selected through thepressure exerted by phagocytosis. In particular, copper homeostasis regulation mechanismsare part of the strategies adopted by pathogenic bacteria to survive phagocytosis. Thesemechanisms vary depending on the ecological niche of each species. Studies on this topichave mainly focused on environmental bacteria and on pathogenic bacteria, especially thosewith an intracellular lifestyle. Copper homeostasis in Bordetella pertussis is at the centre ofthis thesis. This bacterium is the etiologic agent of whooping cough whose peculiarity is to bestrictly dependent on its host. Furthermore, its ecological niche is very limited, as it onlyinfects the human upper respiratory epithelium. This bacterium represents a new model tostudy the role of copper in host-pathogen relationships. This work has revealed the loss ofmost copper homeostasis systems in B. pertussis. An original detoxification mechanism witha composite regulation has been identified that includes the cytoplasmic metallochaperoneCopZ and two enzymes catalyzing detoxification of peroxides. This thesis work has led to thefollowing hypothesis: this system, which is a distinctive feature of the Bordetella genus, isinvolved in survival to phagocytosis, the only situation where the bacterium experiencescopper excess in its ecological niche. The metallo-chaperone CopZ mediates thedetoxification of free copper by complexation, and together the two enzymes decrease theconcentration of hydrogen peroxide, which causes oxidative stress through the Fenton andHaber-Weiss reactions. The co-regulation of this system by copper and hydrogen peroxideresults in a very dynamic and selective activation, specific to phagocytosis-relatedmechanisms. This system offers a sufficiently favorable cost-benefit balance to have beenselected over the course of the evolution of this host-restricted pathogen.This work has led to the identification of copper tolerance mechanisms in B. pertussisand to the description of their evolutionary selection among Bordetellae
Gerlach, Gabriele. "Funktionelle Charakterisierung des BvgASBH-Zwei-Komponentensystems von Bordetella holmesii". [S.l.] : [s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=973411678.
Pełny tekst źródłaTefon, Burcu Emine. "Towards Whole Cell Immunoproteome And Subproteomes Of Bordetella Pertussis". Phd thesis, METU, 2012. http://etd.lib.metu.edu.tr/upload/12614238/index.pdf.
Pełny tekst źródłaBokhari, Syed Habib. "Characterisation and secretion mechanism of Bordetella pertussis autotransporter proteins". Thesis, University of Glasgow, 2002. http://theses.gla.ac.uk/1507/.
Pełny tekst źródłaGarrod, Tracey. "Partial purification and characterisation of Bordetella bronchiseptica dermonecrotic toxin". Thesis, University of Reading, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.239514.
Pełny tekst źródłaFunnell, Simon Gordon Paul. "Mechanisms of colonisation of mammalian tissues by Bordetella pertussis". Thesis, Open University, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.239726.
Pełny tekst źródłaPearce, Alexandra M. "The structure and immunogenicity of fimbriae from Bordetella pertussis". Thesis, Open University, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.333436.
Pełny tekst źródłaLi, Jing-Li. "A molecular study of virulence factors of Bordetella species". Thesis, Open University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303193.
Pełny tekst źródłaBassinet, Laurence. "Bordetella pertussis et épithélium respiratoire : aspects biologiques et cliniques". Paris 12, 2004. https://athena.u-pec.fr/primo-explore/search?query=any,exact,990003948590204611&vid=upec.
Pełny tekst źródłaWhooping cough is a respiratory disease caused by Bordetella pertussis (Bp). Because of waning immunity after vaccination, adult are thought to have been acting as a reservoir for infections in infants, for whom the disease can be serious. We report a pertussis out break among health care workers (HCW) in an hospital with the contamination of 2 adults immunosuppressed patients. Such observations could support the vaccination of HCW. We also studied interactions between human respiratory epithelium and Bp as these cells could constitute a bacterial reservoir in adults. We showed that Bp invades human HTE tracheal apithelial cells line. The invasive capacity of the bacterium is inhibited by the expression of a toxin, the adenylate cyclase-hemolysin (AC-Hly). Interaction of Bp with HTE cells induces the secretion of inflammatory cytokines such as IL-6 by the cells. AC-Hly is responsible of this secretion confirming that this toxin plays an important role in the inflammatory response induced by Bp
Bassinet, Laurence Guiso-Maclouf Nicole. "Bordetella pertussis et épithélium respiratoire aspects biologiques et cliniques /". Créteil : Université de Paris-Val-de-Marne, 2007. http://doxa.scd.univ-paris12.fr:8080/theses-npd/th0394859.pdf.
Pełny tekst źródłaVersion électronique uniquement consultable au sein de l'Université Paris 12 (Intranet). Titre provenant de l'écran-titre. Bibliogr. : 332 réf.
Rambow-Larsen, Amy Alison. "Assembly and secretion of pertussis toxin by bordetella pertussis". Cincinnati, Ohio : University of Cincinnati, 2003. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=ucin1066417734.
Pełny tekst źródłaFong, Winkie Wing Kei. "Genomics and Metagenomics of Bordetella species for Disease Control". Thesis, The University of Sydney, 2020. https://hdl.handle.net/2123/25040.
Pełny tekst źródłaHormozi, E. Kalantar. "Activation and immunogenicity of Bordetella pertussis adenylate cyclase toxin". Thesis, University of Glasgow, 1997. http://theses.gla.ac.uk/30948/.
Pełny tekst źródłaPavic, Espinoza Ivana, Medina Sandy Bendezu, Alzamora Angella Herrera, Maria J. Pons, Adrian V. Hernández, Valle Mendoza Juana Mercedes Del i Universidad Peruana de Ciencias Aplicadas (UPC). "High prevalence of Bordetella pertussis in severe acute respiratory infections in hospitalized children under 5 years in Lima, Peru". Universidad Peruana de Ciencias Aplicadas (UPC), 2015. http://hdl.handle.net/10757/582376.
Pełny tekst źródłaAcute respiratory infections (ARI) are the main cause of morbidity and mortality in children under 5 years worldwide. Bordetella pertussis is a highly contagious bacterium that can cause serious illness, and approximately half of infected infants less than 1 year old are hospitalized. Also, pertussis immunization series is not completed until six months of age, leaving young infants vulnerable to pertussis. In Peru, pertussis is an increasing health problem despite immunization efforts, and the role of B. pertussis in ARI is unknown. We determined the prevalence of B. pertussis among children under 5 years old admitted to Hospital Nacional Cayetano Heredia in Lima with diagnosis of ARI between Jan-2009 and Dec 2010. Epidemiological and clinical features were collected, and presence of B. pertussis was determined by PCR (pertussis toxin and IS481 gene). A total of 596 nasopharyngeal samples among children under 5 years were analyzed. In 114 (19.1%) samples were positive for B. pertussis. 32.5% of sample positive to B. pertussis were diagnosed as viral pneumonia at diagnosis. Importantly, 71.9% of cases were under 12 months of age and 58.8% have been contact with other ARI infected people. Significant differences in clinical symptoms between the total ARI cases and B. pertussis cases were not found. The most frequent symptoms in B. pertussis cases were fever (100%), rhinorrhea 78%, cough 71.9% and respiratory distress 60.5%. One child died due to the infection. B. pertussis cases showed a seasonal distribution with peaks during the months March June and November. This study shows the high prevalence of B. pertussis in infants who were hospitalized due to severe acute respiratory infections in Lima, Peru. Epidemiologic surveillance programs for B. pertussis are essential in the future in Peru
Mir, Cros Alba. "Dinàmica poblacional i deriva antigènica de Bordetella pertussis i el paper d’altres espècies del gènere Bordetella en l’emergència de la tosferina al segle XXI". Doctoral thesis, Universitat Autònoma de Barcelona, 2021. http://hdl.handle.net/10803/673654.
Pełny tekst źródłaLa tosferina es una enfermedad infecciosa aguda causada generalmente por Bordetella pertussis, un cocobacilo gramnegativo, de reservorio exclusivamente humano y con una elevada transmisibilidad por vía aérea. Se caracteriza por presentar un cuadro respiratorio de tos intensa que, a menudo, puede prolongarse durante semanas o meses. Entre las posibles causas implicadas en el resurgimiento de la tosferina, destaca la adaptación de B. pertussis a la inmunidad conferida por las vacunas acelulares (DTPa), las cuales se introdujeron a finales de los años noventa, así como la emergencia de nuevas especies del género Bordetella contra las que las vacunas utilizadas no confieren protección. Por un lado, el proceso de adaptación de B. pertussis a la inmunidad inducida por las DTPa ha evidenciado como, coincidiendo con la introducción de éstas, han aparecido nuevas poblaciones de B. pertussis que han ido reemplazando progresivamente las variantes antigénicas presentes en las composiciones vacunales utilizadas. En este sentido, se ha observado un viraje en la estructura de las poblaciones de B. pertussis circulantes, reflejada por el recambio de los perfiles electroforéticos que han circulado entre los períodos de uso de las vacunas de células completas (DTPw) y las DTPa, así como de la emergencia y posterior predominio del genotipo MT27, el que se ha observado en el 79,3% de los aislados de B. pertussis circulantes tras la introducción de las DTPa. Adicionalmente, en estas poblaciones, se han observado polimorfismos en los genes que codifican la toxina pertusis (ptxA) y en su promotor (ptxP), la pertactina (prn) y la fimbria de tipo 3 (fim3). Así, la combinación alélica ptxA1/prn2/fim3-2, la cual presenta formas antigénicas diferentes a las contenidas en las DTPa, se ha observado como mayoritaria (52,7%) entre los aislados que han circulado en nuestro medio después de la introducción de estas vacunas. Adicionalmente, la presencia mayoritaria (97,7%) del promotor de la toxina pertusis de tipo 3 (ptxP3) condiciona un incremento de la producción de este determinante de virulencia entre los aislados de B. pertussis que lo poseen. Por otra parte, se ha puesto de manifiesto la circulación de aislados de B. pertussis que dejan de expresar el antígeno vacunal de la pertactina (PRN), manteniendo su virulencia y capacidad para producir enfermedad. Éstos han supuesto 27,2% del total, identificándose únicamente en el período de uso de las DTPa. En este sentido, la aparición y diseminación exitosa, de un clúster de aislados que han presentado una deleción en el promotor y parte del gen que codifica este determinante de virulencia (58,1%, prn::del(-292, 1340)), ha evidenciado la expansión de los aislados de B. pertussis que no expresan PRN. De este modo, la diseminación de aislados de B. pertussis que expresan antígenos vacunales que poseen epítopos no reconocidos por los anticuerpos generados mediante el uso de las DTPa, así como la pérdida de la expresión de estos antígenos como importantes mecanismos de adaptación y evasión de la inmunidad conferida por las vacunas utilizadas, parece ser un mecanismo que puede comprometer su eficacia. Finalmente, la descripción de la emergencia de B. holmesii como agente causal de la tosferina, observándose una prevalencia de este microorganismo del 3,9% en 2015 y del 8,8% en 2016, demuestra que su circulación ha podido contribuir en el resurgimiento de la tosferina. En relación con éstos, no se han observado diferencias en las características clínicas-epidemiológicas de los casos de B. holmesii en comparación con aquellos causados por B. pertussis, ni se han evidenciado complicaciones o recidivas después de su tratamiento con azitromicina. Así, el conjunto de observaciones refuerza la necesidad de adaptar las herramientas diagnósticas actuales para la identificación correcta de las etiologías de la tosferina.
Whooping cough is an acute infectious disease usually caused by Bordetella pertussis, a gram-negative coccobacillus, with an exclusively human reservoir and high airborne transmissibility. It is characterized by presenting a respiratory picture of intense cough that can often last for weeks or months. Among the possible causes that are involved in the pertussis resurgence, the adaptation of B. pertussis to the immunity conferred by acellular vaccines (DTPa), which were introduced in the late 1990s, as well as the emergence of new species of the genus Bordetella, against which the vaccines used do not confer protection, are some of the most important ones that have be considered. On the one hand, the process of adaptation of B. pertussis to the immunity induced by DTPa has been evidenced by the new populations of B. pertussis that have appeared and have gradually replaced the antigenic variants present in the vaccine, concurrently with its introduction. In this sense, a change in the structure of circulating populations has been observed, reflected by the replacement of the electrophoretic profiles that have circulated within the different vaccine periods. Is remarkable the emergence and subsequent predominance of the MT27 genotype, which has been observed in 79.3% of circulating B. pertussis isolates after the introduction of DTPa. Additionally, in these populations, polymorphisms in the genes encoding the pertussis toxin (ptxA) and its promoter (ptxP), pertactin (prn) and fimbria type 3 (fim3). Thus, the allelic combination ptxA1/prn2/fim3-2, which include different antigenic forms than those contained in DTPa, has been observed as the most prevalent (52.7%) among the isolates that have circulated in our environment after the introduction of this type of vaccine. Additionally, a high prevalence (97.7%) of the type 3 pertussis toxin promoter (ptxP3), which induces an increase in the production of this virulence determinant in these B. pertussis isolates, is found in isolates obtained after the DTPa introduction. Other factor that has evidenced the B. pertussis adaptation is the emergence of pertactin (PRN) negative isolates maintaining its virulence and ability to produce disease. In our study, PRN-negative isolates have accounted for the 27.2% of the total studied isolates. They have been identified only during the period of use of DTPa. In this sense, the emergence and successful dissemination of a cluster of isolates that possess a deletion in the promoter and part of the gene encoding this determinant of virulence (58.1%, prn::del(-292 , 1340)), has evidenced the expansion of most of the B. pertussis isolates that do not express PRN. Thus, the spread of B. pertussis isolates expressing vaccine antigens possessing epitopes not recognized by antibodies generated by the use of DTPa, as well as the loss of expression of these antigens as an important mechanism of adaptation and evasion of immunity conferred by pertussis vaccines, seems to be a mechanism contributing to compromise the vaccine effectiveness. Finally, the description of the emergence of B. holmesii as the causative agent of pertussis, observing a prevalence of this microorganism of 3.9% in 2015 and 8.8% in 2016, shows that its circulation has been able to contribute to the pertussis resurgence. In relation to these, no differences were observed in the clinical-epidemiological characteristics of the cases by B. holmesii and those caused by B. pertussis, nor were there any complications or relapses after its treatment with azithromycin. Accurate diagnosis of the causative agent is crucial to determine the incidence and prevalence of the microbial species involved, to assess its contribution to the epidemiology of whooping cough, to evaluate whether specific antimicrobial drug treatments should be implemented and, in terms of public health, to assess the efficacy of the pertussis vaccine.
Universitat Autònoma de Barcelona. Programa de Doctorat en Microbiologia
Iscan, Elvin. "Analysis Of Cross-immune Reaction Between Strains Of Bordetella Pertussis". Master's thesis, METU, 2009. http://etd.lib.metu.edu.tr/upload/12611359/index.pdf.
Pełny tekst źródłaTahoma I&rdquo
with those of two other strains, namely &ldquo
Saadet&rdquo
and &ldquo
Nursel&rdquo
, which are the local isolates that have been preferred as the vaccine strains for many years in our country for their ability to provide a better protection. Of a total of 38 immunogenic proteins identified, 14 were shown to be the novel antigens for B. pertussis. Among 14 proteins, one was detected as immunogenic in only Tohama I strain where two proteins were specific for Nursel strain. Among the strains compared, Saadet strain had the highest antigenic variety, than the others.
Al-Fellah, Giamal Nouri. "Inactivation of Bordetella pertussis by rat lung lavage fluids (LLF)". Thesis, University of Glasgow, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263427.
Pełny tekst źródłaBrotherston, Christopher. "Interaction of Bordetella pertussis adenylate cyclase toxin with target cells". Thesis, University of Glasgow, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263429.
Pełny tekst źródłaBinns, Sarah Helen. "Studies on the epidemiology of Bordetella bronchiseptica infection in cats". Thesis, University of Liverpool, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243267.
Pełny tekst źródłaZARROUK, HASSAN. "Etude de la structure d'endotoxines de bacteries du genre bordetella". Paris 11, 1998. http://www.theses.fr/1998PA112051.
Pełny tekst źródłaHettiarachchige, Amali. "Detection of Bordetella Infection in a Paediatric Population at CHW". Thesis, The University of Sydney, 2015. http://hdl.handle.net/2123/14872.
Pełny tekst źródłaLeusch, Mark Steven. "Purification and characterization of adenylate cyclase toxin from Bordetella pertussis". Diss., The University of Arizona, 1990. http://hdl.handle.net/10150/184998.
Pełny tekst źródłaSteed, Lisa Lovett. "Host-parasite interactions between Bordetella pertussis and human polymorphonuclear leukocytes". Diss., The University of Arizona, 1991. http://hdl.handle.net/10150/185641.
Pełny tekst źródłaEdwards, Jessica. "Initial Interactions between Bordetella bronchiseptica and Tracheal Epithelial Cell Cilia". Diss., The University of Arizona, 2006. http://hdl.handle.net/10150/195708.
Pełny tekst źródłaFelizardo, Maria Roberta. "Isolamento e caracterização de amostras de Bordetella bronchiseptica através da eletroforese em gel de campo pulsado (PFGE)". Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-13032012-140016/.
Pełny tekst źródłaBordetella bronchiseptica is one of the etiologic agents of kennel cough, cat superior respiratory tract infections, swine bronchopneumonia, being frequently isolated from rabbits and laboratory animals. The impact of this agent in human health is still underestimated; the risk of immunosuppressed persons who have contact with domestic animals exists and is reported in literature. In Brasil, there are no studies characterizing the agent isolated from pet species. The knowledge of epidemiological aspects of Bordetella bronchiseptica is of fundamental importance to adopt effective control measures and prevention of human and domestic animals, in that sense, studies of molecular epidemiology are very relevant. This study aims to characterize strains of Bordetella bronchiseptica from dogs and cats through pulsed field gel electrophoresis (PFGE) and antimicrobial resistance profile. Forty five strains from three dogs and eleven cats from different catteries, shelters, and veterinary clinics were separated in seven profiles based in resistance patterns and fourteen genotypic profiles through PFGE.
Herrou, Julien. "Etude du régulateur central de la virulence, BvgA/S, chez Bordetella pertussis, l'agent de la coqueluche". Lille 2, 2008. http://www.theses.fr/2008LIL2S038.
Pełny tekst źródłaLechner, Melanie. "Charakterisierung des Umweltkeims Bordetella petrii : Untersuchungen zur genomischen Variabilität und zum Bvg Regulon". kostenfrei, 2008. http://www.opus-bayern.de/uni-wuerzburg/volltexte/2009/3439/.
Pełny tekst źródłaPons, Maria J., Cl觃udia Gomes, Carlos Bada, Isabel Reyes, Valle Mendoza Juana Del i Joaquim Ruiz. "Characterization of two Achromobacter xylosoxidans isolates from patients with pertussis-like symptoms". Elsevier B.V, 2015. http://hdl.handle.net/10757/554305.
Pełny tekst źródłaObjective: To characterize two Achromobacter xylosoxidans recovered from 2 patients diagnosed with pertussis during a Bordetella pertussis surveillance program. Methods: Nasopharyngeal swabs from 2 children under 1 year of age with clinical suspicion of pertussis were analyzed by culture and PCR. Results: Two Achromobacter xylosoxidans A8, closely related to Bordetella spp. were recovered from 2 patients diagnosed of pertussis, both carrying the ptxA gene and IS418 the pertussis toxin encoding gene. Subsequently, antibiotic susceptibility was evaluated by disk-diffusion method and by PCR. Conclusions: Although more detailed studies are needed, the present data highlight the possibility that Achromobacter xylosoxidans, closely related Bordetella pertussis microorganisms and not covered under the vaccine umbrella, might also result in cases of whooping cough. Thereby further surveillance is necessary to determine the extension and relevance of their pathogenic role in order to discriminate their real public health implication.