Gotowa bibliografia na temat „Bivalves – Mortalité”

Fouling by the recently established zebra mussel (Dreissena polymorpha) causes differential effects among bivalve communities. In 3-mo experiments and surveys conducted in western Lake Erie during 1990, two native bivalves, Lampsilis radiata and Amblema plicata, showed consistent differences in mortality and biochemical indices of fitness in response to fouling by D. polymorpha. Lampsilis radiata was very sensitive to fouling, experiencing high mortality and reduced fitness in experiments and natural populations. In field experiments, female L. radiata suffered higher mortality and lower fitness than males. Amblema plicata was less sensitive to fouling; fitness was reduced in experimentally fouled A. plicata, but mortality and fitness in natural populations were not affected by D. polymorpha. Six species of native bivalves showed marked differences in mortality rates at three sites surveyed in western Lake Erie. Mortality was higher in the subfamilies Anodontinae and Lampsilinae (including L. radiata) than in Ambleminae (including A. plicata) at all three sites, suggesting that differences in life history strategy and shell morphology among subfamilies may be responsible for differential responses to fouling. These data suggest that fouling by D. polymorpha will result in profound changes in native bivalve community structure.

The fan mussel, Pinna nobilis, represents the largest bivalve endemic to the Mediterranean Sea. Since 2016, dramatic mass mortality of this species has been observed in several areas. The first surveys suggested that Haplosporidium pinnae (currently considered species-specific) was the main etiological agent, but recent studies have indicated that a multifactorial disease may be responsible for this phenomenon. In this study, we performed molecular diagnostic analyses on P. nobilis, P. rudis, and bivalve heterologous host species from the island of Sardinia to shed further light on the pathogens involved in the mass mortality. The results support the occurrence of a multifactorial disease and that Mycobacterium spp. and H. pinnae are not necessarily associated with the illness. Indeed, our analyses revealed that H. pinnae is not species-specific for P. nobilis, as it was present in other bivalves at least three years before the mass mortality began, and species of Mycobacterium were also found in healthy individuals of P. nobilis and P. rudis. We also detected the species Rhodococcus erythropolis, representing the first report in fan mussels of a bacterium other than Mycobacterium spp. and Vibrio spp. These results depict a complicated scenario, further demonstrating how the P. nobilis mass mortality event is far from being fully understood.

Background: The effects of climate change negatively affect marine bivalves’ health. Lately, the intensity of marine heatwaves poses serious threats to the aquatic environment setting of high-risk bivalve farming. Since temperature increases can jeopardize bivalves’ immunity response, pathogen infection becomes more evident. Reactive oxygen species (ROS) production, increased during the process of phagocytosis, is confronted by the animals’ antioxidant defense system. However, apart from pathogenic infections, antioxidant defense responses are also induced by seawater temperature increases; Methods: To enlighten the antioxidant status of Mytilus galloprovincialis originating from mortality events enhanced by intense heatwaves in Thermaikos Gulf, northern Greece, along with Marteilia refringens infection, we examined the expression of genes related to antioxidant defense (catalase, CuSOD and mt10) along with the lipid peroxidation levels and activity levels of antioxidant enzymes (catalase, SOD and glutathione reductase); Results: Our results exhibited increased levels of all these biomarkers. This increase was intensified in the Marteilia infected individuals; Conclusions: Our findings shed light on the oxidative and antioxidant status of farmed mussels led to mortality in the context of Marteilia infection. The latter is augmented by the synergistic effect of heatwaves causing a significant increase in oxidative damage and subsequent antioxidant defense.

The densities of two common intertidal/shallow subtidal bivalves, Soletellina alba and Arthritica helmsi, were sampled in vegetated and unvegetated habitats of the Hopkins River estuary on three occasions during the autumn/winter 1995. Winter flooding coincided with mass mortalities of the infaunal bivalve S. alba, but not A. helmsi. Mortalities were apparent for individuals living deeper in the sediment (≈35 cm) in vegetated and unvegetated habitats, but small S. alba (<1 mm) were less susceptible to mortality than larger individuals (>1 mm). Mortalities were similar across different habitat types and sediment depths, and at multiple sites within close proximity to the estuary mouth.

Mass mortality of bivalve clams along with some other marine fauna was observed during the month of May 2016, mainly consisting of the clams (Mactra aequisulcata) of family Veneridae surfaced over the Clifton coast. The occurrence of mass mortality of the venerid clams Mactra aequisulcata and other marine life has been studied due to the toxicity of chlorinated hydrocarbons (DDTs, HCHs, hexa chloro benzene (HCB), chlordane, dieldrin (4-chlorophenyl), methanol (TCPMOH) and heptachlor epoxide and polychlorinated biphenyls (PCBs). Significant compositional characteristics of PCBs and DDTs were found in all dead clams and other fauna including fish (Johnius carutta), crabs (Portunus pelagicus), gastropods (Babylonia spirata), bivalves (Anadara antiquata), (Mactra aequisulcata) and the pen shell (Atrina pectinata) from Karachi coast, Pakistan. Mean concentrations (ranges) of organochlorine pesticides (OCPs) in crab (Portunus pelagicus) and fish samples were 1.1 (<0.01-1.5), 0.22 (<0.01-1.1) and 0.14 (<0.01-1.3) /µg/g. Those in, bivalves (Anadara antiquata) and pen shell (Atrina pectinata), clams (Mactra aequisulcata) were 0.09 (<0.02-1.2), 0.22 (<0.02-1.3) /µg and 0.13 (<0.01-0.27), respectively. The heptachlor epoxide was found in highest concentration in clam samples (25.00 ± 30.92) µg/g, (wet weight) however, in bivalves, (2.30 µg/g, (wet weight), which were higher than those in other gastropods. Polychlorinated biphenyls (PCBs) concentrations were also measured in the same samples of gastropods (Babylonia spirata), fish (Johnius carutta), crab (Portunus pelagicus), clams (Mactra aequisulcata), bivalves (Anadara antiquata) and the pen shell (Atrina pectinata) to determine the possible cause of mass mortality. The DDT to metabolites (DDD & DDE) concentration ratios exceeded upto 1.0 in the sessile fauna that is clams (Mactra aequisulcata) and the pen shell (Atrina pectinata) from Karachi coast. These organisms also exhibited dichloro diphenyl trichloroethane (DDT) inputs, whereas dichloro diphenyl trichloroethane (DDD) was found to be in degraded component and PCBs were generally in low concentrations. The concentrations of DDTs were higher than the ERL guidelines in the coastal areas of Karachi, suggesting that there is potential of ecological risk present in the prevailing environment.

All else being equal, species with short life spans are expected to be overrepresented in time-averaged death assemblages relative to their standing abundance in the living community, but the magnitude of the distortion of proportional abundance and assemblage evenness has received little attention. Here, information from 30 data sets on the living and dead abundances of marine bivalves in local habitats is combined with a global compilation of bivalve life spans to determine whether bias from mortality rate can explain observed differences in species proportional abundances. Although bivalve maximum life spans range from one to 75 years in these data sets, indicating annual mortality rates of 0.97 to 0.09, the “life span bias” (LB) of a species–the difference between its proportional abundance expected dead and that observed alive–is consistently small in magnitude (average change <2%, maximum about 20%) and random in sign relative to observed discordance (OD = difference between that species' proportional abundance observed dead and that observed alive). The aggregate result for 413 living species occurrences is a significantly positive but weak correlation of OD to LB, with only 10% of variation in OD explained. The model performs better among longer-lived species than among shorter-lived species, probably because longer-lived species conform better to the model assumption that species maintain a constant proportional abundance in the living assemblage over time. Among individual data sets, only seven exhibit significant positive correlations between OD and LB. The model also under-predicts the cases where a death assemblage is dominated by a species that is shorter lived than the dominant species in the living assemblage, indicating that some factor(s) other than or in addition to mortality rate is responsible for OD. We can find no evidence of preservational bias linked to life span, for example through body size. This negative outcome reflects a weak biological relationship between life span and living abundance among bivalves in local habitats, contrary to the terrestrial paradigm, and points toward a simpler model of time-averaged death assemblage formation where higher abundances reflect (under-sampled) past populations. Contrary to long-held expectations, variation in population turnover among species is not a major source of taphonomic bias in time-averaged death assemblages among bivalves and perhaps among other marine groups: bias must arise largely from other factors.

Abstract Control strategies for established populations of invasive alien species can be costly and complex endeavours, which are frequently unsuccessful. Therefore, rapid-reaction techniques that are capable of maximising efficacy whilst minimising environmental damage are urgently required. The Asian clam (Corbicula fluminea Müller, 1774), and the zebra mussel (Dreissena polymorpha Pallas, 1771), are invaders capable of adversely affecting the functioning and biodiversity of freshwater ecosystems. Despite efforts to implement substantial population-control measures, both species continue to spread and persist within freshwater environments. As bivalve beds often become exposed during low-water conditions, this study examined the efficacy of steam-spray (≥100 °C, 350 kPa) and open-flame burn treatments (~1000 °C) to kill exposed individuals. Direct steam exposure lasting for 5 min caused 100% mortality of C. fluminea buried at a depth of 3 cm. Further, combined rake and thermal shock treatments, whereby the substrate is disturbed between each application of either a steam or open flame, caused 100% mortality of C. fluminea specimens residing within a 4-cm deep substrate patch, following three consecutive treatment applications. However, deeper 8-cm patches and water-saturated substrate reduced maximum bivalve species mortality rates to 77% and 70%, respectively. Finally, 100% of D. polymorpha specimens were killed following exposure to steam and open-flame treatments lasting for 30 s and 5 s, respectively. Overall, our results confirm the efficacy of thermal shock treatments as a potential tool for substantial control of low-water-exposed bivalves. Although promising, our results require validation through upscaling to field application, with consideration of other substrate types, increased substrate depth, greater bivalve densities, non-target and long-term treatment effects.

During July 1984, large numbers of the sandy beach bivalve Donax vittatus (da Costa) were found either stranded in the intertidal, or washing to and fro in the surf on beaches at Dornoch, on the east coast of Scotland. The affected bivalves all carried large growths of several species of algae. The normal burrowing and recovery responses of the bivalves were unable to overcome the increased drag resulting from these epizoic algal colonies so that wave action caused dislodgement from the sand and eventually, stranding and death from dehydration. The resulting mortality affected mainly older individuals in the population; younger individuals had very small or no attached algae and were not dislodged.The algal growths were complex and showed three stages in their development: primary settlements formed generally short tufts or mats attached to the posterior tip of die shell; secondary ‘dominant’ species were also attached to die shell but formed larger growths; tertiary species were attached as epiphytes to the primary and secondary growths. At least eight species of algae were involved. Juvenile mussels (Mytilus edulis L.) were also present in many cases attached to the weed, adding further to die drag.During 1985, at Dornoch, secondary and tertiary algal species developed progressively from overwintering primary settlements, but the size of die algal colonies which developed was generally smaller dian in 1984 and diere was apparently little dislodgement and mortality.Donax vittatus populations on other beaches in die Moray Firdi, and on most other Scottish beaches examined, had fewer individuals with attached algae, and diose affected carried only relatively small colonies which did not prevent normal burrowing. Complex algal colonies, showing even greater species diversity than those of Dornoch were, however, found on D. vittatus on beaches in South Harris, Outer Hebrides, during September 1984.

The Baltic tellin Limecola balthica is one of the most common bivalves in intertidal areas in the Northern Hemisphere. Over the last 2 decades, the species has been suffering from a decrease in adult survival in the European Wadden Sea. While several factors such as global warming and fisheries have been suggested to influence the population dynamics of this bivalve mollusc, the potential role of diseases has never been investigated. In this study, we investigated whether disseminated neoplasia, a common proliferative disorder in bivalve molluscs, could play a potential role in the recent population decline of Baltic tellins in the Wadden Sea. We conducted a field survey in the Dutch Wadden Sea to (1) determine whether the disease occurs in Baltic tellins in the Wadden Sea and (2) quantify the occurrence and severity of the disease via histology. Disseminated neoplasia occurred in L. balthica at each of the 10 sampled locations with very high prevalences (21-89%) compared to those reported elsewhere for this species. The highest severity category was found in 8 to 87% of affected individuals, with severity generally increasing with prevalence. Disseminated neoplasia usually increases mortality among affected individuals and may also be associated with important sub-lethal effects, especially regarding gametogenesis. Thus, we suggest that disseminated neoplasia may play a key role in the population dynamics of the Baltic tellin, the extent of which remains to be investigated in future studies.

Abstract In the 80ies, in Southern Europe and in particular in Ria Formosa there was an episode of heavy mortality of the economically relevant clam Ruditapes (R.) decussatus associated with a debilitating disease (Perkinsosis) caused by Perkinsus olseni. This protozoan parasite was poorly known concerning its’ differential transcriptome in response to its host, R. decussatus. This laboratory available protozoan system was used to identify parasite genes related to host interaction. Beyond the application of molecular biology technologies and methodologies, only the help of Bioinformatics tools allowed to analyze the results of the study. The strategy started with SSH technique, allowing the identification of parasite up-regulated genes in response to its natural host, then a macroarray was constructed and hybridized to characterize the parasite genes expression when exposed to bivalves hemolymph from permissive host (R. decussatus), resistant host (R. philippinarum) and non permissive bivalve (Donax trunculus) that cohabit in the same or adjacent habitats in Southern Portugal. Genes and respective peptides full molecular characterization depended on several Bioinformatic tools application. Also a new Bioinformatic tool was developed.

Les zones côtières constituent des milieux d’interface important entre les écosystèmes marins et terrestres et sont peuplées par des communautés vivantes abondantes. Parmi ces zones côtières, le littoral picto-charentais constitue une zone de fortes activités économiques liées, entre autres, à l'aquaculture, à la pêche et au tourisme, nécessitant ainsi une bonne qualité des eaux et de l’environnement. Or, cette zone littorale est sous l’influence de nombreux apports de contaminants : produits phytosanitaires, hydrocarbures, métaux lourds, etc., dont l’eau constitue le principal vecteur. Afin de préserver les Pertuis, le Parc Naturel Marin a mis en place un plan de gestion déterminant les mesures de protection, de connaissance, de mise en valeur et de développement durable à mettre en œuvre pour les 15 ans à venir. Le projet QUALIPERTUIS, a pour objectif d’associer une phase de tests en laboratoire et des mesures sur site afin d’apporter un regard nouveau sur les causes de mortalités des bivalves, ainsi que de comprendre plus largement l’impact des facteurs environnementaux influençant l’état sanitaire de ces derniers dans les zones portuaires et les eaux côtières. En partenariat avec le Port de Plaisance de La Rochelle et Port Atlantique La Rochelle, des études de biosurveillances environnementales ont été mises en place dans les espaces portuaires et dans les Pertuis avec trois espèces de mollusques bivalves : le pétoncle noir Mimachlamys varia, l’huitre creuse Crassostrea gigas et la moule bleue Mytilus edulis. Des suivis environnementaux saisonniers ont ainsi été réalisés sur un total de treize sites d’études, répartis dans les espaces portuaires et les Pertuis
Coastal areas are important interface environments between marine and terrestrial ecosystems and are populated by abundant living communities. Among these coastal areas, the Charente coastline is an area of strong economic activities related, among others, to aquaculture, fishing and tourism, thus requiring a good quality of water and environment. However, this coastal area is under the influence of many contaminants: phytosanitary products, hydrocarbons, heavy metals, etc., of which water is the main vector. In order to preserve the Pertuis, the Marine Natural Park has set up a management plan determining the measures of protection, knowledge, enhancement and sustainable development to be implemented for the next 15 years. The QUALIPERTUIS project aims to combine a phase of laboratory tests and on-site measurements in order to provide a new perspective on the causes of bivalve mortality, as well as to understand more broadly the impact of environmental factors influencing the sanitary state of bivalves in port areas and coastal waters. In partnership with the La Rochelle Marina and Port Atlantique La Rochelle, environmental biomonitoring studies have been set up in port areas and in the Pertuis with three species of bivalve molluscs: the black scallop Mimachlamys varia, the hollow oyster Crassostrea gigas and the blue mussel Mytilus edulis. Seasonal environmental monitoring has been carried out on a total of thirteen study sites, distributed in the port areas and the Pertuis

I molluschi bivalvi sono animali filtratori che possono accumulare nei loro tessuti anche microrganismi patogeni presenti nell'ambiente circostante (batteri, virus e parassiti), quest’ultimi possono causare uno stato di malattia che nei casi più servi può portare alla morte, evento percepita dal molluschicoltore come “mortalità anomala”. Negli ultimi anni è stato dimostrato come siano soprattutto fattori ambientali, quali variazioni di temperatura e salinità dell’acqua, a concorrere nel favorire l’indebolimento del mollusco a favore del patogeno. La mortalità anomala, dipende dall’cambiamento dei parametri sopra elencati, dalla concentrazione del patogeno e dallo stress dell’organismo, il quale diventa più sensibile ai diversi patogeni come i batteri o/e virus ed anche può causare una crescita batterica più rapida può così essere con-causa di alti tassi di mortalità. Lo stress può anche favorire lo sviluppo di agenti patogeni opportunisti che si trovano nella comunità batterica del mollusco o nell’acqua. L’oggetto principale di questa tesi di dottorato è l’indagine delle cause che hanno portato a diversi episodi di mortalità in molluschi bivalvi. Una parte di questi microrganismi è di difficile coltivazione in laboratorio a causa delle loro particolari esigenze, altri non sono coltivabili affatto. Da qui deriva la necessità di sviluppare nuovi metodi di analisi come la metagenomica in alternativa e complementari all’utilizzo di metodiche tradizionali come quelle istologiche e microbiologiche. Anche un approccio MALDI-TOF.

Una de les principals limitacions a la que s‘enfronta la producció de mol·luscs bivalves és la prevenció i control de malalties, que es dispersen, principalment, pel moviment d‘estocs. A les Balears, hem trobat paràsits de bivalves poc patogènics com Bucephalus haimeanus, Mytilicola intestinalis, i metacercàries de tremàtodes. També altres que suposen un risc per a la producció, com Marteilia refringens, Perkinsus mediterraneus i P. olseni. La presència de M. refringens és una greu amenaça, doncs produeix desordres fisiològics que poden matar l‘hoste. Probablement, als anys 80, la desaparició dels bancs ostrícoles va ser causada per aquesta malaltia. Els musclos també són susceptibles a la infecció per M. refringens, però són resistents a la malaltia. L‘any 2004, es va detectar una mortalitat al banc de la rossellona (Chamelea gallina) de s‘Arenal de Palma de Mallorca. Per detectar l‘espècie causant de la malaltia es va recórrer a tècniques histològiques, hibridació in situ, PCR i PCR niada. La seqüència obtinguda a partir d‘un bloc de parafina va mostrar un 99,1 % de similitud amb les M. refringens tipus O. D‘aquesta manera es va concloure que l‘agent etiològic era M. refringens i posteriorment que la prevalença era del 55,1%. La ubicació específica de les cèl·lules de Marteilia refringens en els teixits de la rossellona es va determinar per hibridació in situ, trobant totes les fases conegudes, inclosa la d‘esporulació, el que demostra que el paràsit completa la infecció a la rossellona. Perkinsus mediterraneus infecta a una gran varietat de mol·luscs bivalves a l‘arxipèlag balear: Ostrea edulis, Mimachlamys varia, Arca noae, Chamelea gallina, Pinna nobilis i Venus verrucosa, sense mortalitats associades a aquest paràsit. Perkinus olseni sols s‘ha detectat en V. verrucosa, però en el present estudi no l‘hem trobat. La recerca de Perkinsus spp. s‘ha realitzat mitjançant RFTM i la determinació de l‘espècie per PCR-RFLP i seqüenciació. La prevalença de P. mediterraneus ha estat semblant a la d‘altres espècies de Perkinsus i la dinàmica de la seva infecció es semblant a la de P. marinus en la badia de Chesapeake, amb valors màxims de detecció del paràsit al setembre i octubre, després del màxim estival de temperatura, i regressió de la infecció a l‘hivern. Hem trobat 12 haplotips de P. mediterraneus amb una elevada similitud genètica. Les diferències es fan majors en incloure seqüències procedents de la bases de dades del GenBank, augmentant el nombre d‘haplotips a 24. Les anàlisis filogenètiques han detectat, en conjunt, tres grups diferents d‘O. edulis de Menorca, que es diferencien d‘altres llinatges coespecífics. Les anàlisis recolzen aquesta diferenciació entre les poblacions de Menorca i Mallorca, la qual sembla, en bona part deguda a l‘aïllament geogràfic del port de Maó. Malgrat això, altres factors, com la variabilitat ambiental, diferents localitats i dates de detecció, la translocació d‘animals, l‘activitat humana, etc, poden tenir certa influència. Al Mediterrani occidental es troben tres espècies de Perkinsus. Malgrat que es coneix que es produeixen co-infeccions a l‘escopinya gravada del port de Maó amb P. olseni i P. mediterraneus, no hem trobat cap cas, ni tampoc bivalves afectats per P. chesapeaki, espècie que s'ha trobat al delta de l'Ebre. A diferents mostreigs de C. gallina hem detectat la presència de M. refringens i P. mediterraneus. Encara que no hem trobat co-infecció, aquesta no es pot descartar, perquè els individus són molt joves i possiblement ambdós patògens estiguin a fases inicials d‘infecció.Tenint en compte que l‘esporulació de M. refringens s‘inicia quan la temperatura de l‘aigua és de 17ºC (mes de maig), que les zoospores de P. mediterraneus apareixen més tard (setembre-octubre) i que les mortalitats es detecten al juny-juliol, és més probable que la causa de la mortalitat sigui la marteiliosi.
Una de las principales limitaciones a la que se enfrenta la producción de moluscos bivalvos es la prevención y control de enfermedades, dispersadas, principalmente, por movimiento de partidas. En las Baleares, hemos encontrado parásitos de bivalvos poco patogénicos como Bucephalus haimeanus, Mytilicola intestinalis y metacercarias de tremátodos. También otros que suponen un riesgo para la producción, como Marteilia refringens, Perkinsus mediterraneus y P. olseni. La presencia de M. refringens es una grave amenaza, pues produce desórdenes fisiológicos que pueden matar al huésped. Probablemente, en los años 80, la desaparición de los bancos ostrícolas fue causada por esta enfermedad. Los mejillones también són susceptibles a la infección por M. refringens, pero són resistentes a la enfermedad. En 2004, se detectó una mortalidad en el banco de la chirla (Chamelea gallina) de s‘Arenal de Palma de Mallorca. Para detectar la especie causante de la enfermedad se recurrió a técnicas histológicas, hibridación in situ, PCR y PCR anidada. La secuencia obtenida a partir de un bloque de parafina mostró un 99,1% de similitud con M. refringens tipo O. De esta manera se concluyó que el agente etiológico era M. refringens y posteriormente, que la prevalencia era del 55,1%. La ubicación específica de las células de Marteilia refringens en los tejidos de la chirla se determinó por hibridación in situ, encontrando todas las fases conocidas, incluida la de esporulación, lo que demuestra que el parásito completa la infección en la chirla. Perkinsus mediterraneus infecta a una gran variedad de moluscos bivalvos en el archipiélago balear: Ostrea edulis, Mimachlamys varia, Arca noae, Chamelea gallina, Pinna nobilis y Venus verrucosa, sin mortalidades asociadas a este parásito. Perkinsus olseni sólo se ha detectado en V. verrucosa, pero en el presente estudio no lo hemos encontrado. La búsqueda de Perkinsus spp. se ha realizado mediante RFTM y la determinación de la especie por PCR-RFLP y secuenciación. La prevalencia de P. mediterraneus ha sido similar a la de otras especies de Perkinsus y la dinámica de su infección es similar a la de P. marinus en la bahía de Chesapeake, con valores máximos de detección del parásito en septiembre y octubre, después del máximo estival de temperatura, y regresión de la infección en invierno. Hemos encontrado 12 haplotipos de P. mediterraneus con una elevada similitud genética. Las diferencias se hacen mayores al incluir secuencias procedentes de la bases de datos del GenBank, aumentando el número de haplotipos a 24. Los análisis filogenéticos han detectado, en conjunto, tres grupos diferentes de O. edulis de Menorca, que se diferencian de otros linajes coespecíficos. Los análisis apoyan esta diferenciación entre las poblaciones de Menorca y Mallorca, la cual parece, en buena parte debida al aislamiento geográfico del puerto de Mahón. Sin embargo, otros factores, como la variabilidad ambiental, diferentes localidades y fechas de detección, la translocación de animales, la actividad humana, etc, pueden tener cierta influencia. En el Mediterráneo occidental se encuentran tres especies de Perkinsus. Aunque se conoce que se producen coinfecciones en la escupiña grabada del puerto de Mahón con P. olseni y P. mediterraneus, no hemos encontrado ningún caso, ni tampoco bivalvos afectados por P. chesapeaki, especie que se ha detectado en el delta del Ebro. En diferentes muestreos de C. gallina hemos detectado la presencia de M. refringens y P. mediterraneus. Aunque no hemos encontrado coinfección, ésta no se puede descartar, porque los individuos són muy jóvenes y posiblemente ambos patógenos estén en las fases iniciales de infección. Teniendo en cuenta que la esporulación de M. refringens se inicia cuando la temperatura del agua es de 17ºC (mes de mayo), que las zoosporas de P. mediterraneus aparecen más tarde (septiembre-octubre) y que la mortalidad se detecta en junio-julio, lo más probable es que la causa sea la marteiliosis
One of the main issues in bivalve mollusc production is the prevention and control of diseases, scattered mainly by stock movements. In the Balearic Islands, we found low pathogenic bivalve parasites as Bucephalus haimeanus, Mytilicola intestinalis and trematode metacercariae. Furthermore, we found others that are a threat for their welfare, like Marteilia refringens, Perkinsus mediterraneus and P. olseni. M. refringens is a serious threat, because it causes physiological disorders that could kill the host. Presumably, in the 80s, this disease was the agent of oyster banks die out. Mussels are susceptible to the infection, but they are resistant to the disease. Mass mortality was detected in 2004 at the striped Venus shell (Chamelea gallina) bed in S‘Arenal beach. We use histological techniques, in situ hybridization, PCR and nested PCR to detect the disease agent. From a paraffin block we retrieved a sequence which showed 99.1% similarity with M. refringens type O. Thus, it was concluded that the aetiological agent was M. refringens. Subsequently, we found a prevalence of 55.1%. Marteilia refringens specific location in striped Venus shell tissues was determined by in situ hybridization. We observed all known stages, including sporulation, thus this parasite could complete its vital cycle in C. gallina, and so striped Venus shell should be considered as a new host of M. refringens. A wide bivalve mollusc variety is infected by Perkinsus mediterraneus in Balearic Islands: Ostrea edulis, Mimachlamys varia, Arca noae, Chamelea gallina, Pinna nobilis and Venus verrucosa, but they are not killed by this parasite. Perkinsus olseni has only been detected in V. verrucosa from Mahon harbour, although we have not detected it in another bivalve species. Perkinsus spp. search was performed using RFTM and species was established by PCR-RFLP and sequencing. P. mediterraneus prevalence was similar to other Perkinsus species and their infection dynamics is like P. marinus’ in Chesapeake Bay, with maximum detection values in September and October, after summer peak temperature with infection regression in winter. We have found 12 P. mediterraneus haplotypes, all of them sharing a high similarity. Differences grow up when we added GenBank sequences. Then, the haplotype number raised 24. Three groups of O. edulis from Minorca were revealed by phylogenetic analyses which are different from other co-specifics lineages. Analysis supported this differentiation among populations from Minorca and Majorca. This differentiation could be due to Mahon harbour geographic isolation. Nevertheless, another factors, such environmental variability, different detection locations and dates, animal translocations, human activity, etc., might also have some influence. There are three Perkinsus species at the western Mediterranean. Although it is known that co-infections between P. olseni and P. mediterraneus can happen in warty Venus shell from Mahon harbour, we have not found any occurrence. Furthermore, we have not detected infection by P. chesapeaki, although it has been found in the Ebro delta. We have found out M. refringens and P. mediterraneus presence in different C. gallina samples. Although coinfection has not been found, we cannot discard it, because individuals are very young and perhaps both pathogens are in early infection stages. Given that M. refringens sporulation starts when water temperature is 17ºC (May), P. mediterraneus zoospores appear later (September-October) and mortality is detected in June-July, in this way, marteiliosis might be the candidate.

Le virus ostreid herpesvirus type 1 (OsHV-1), peut être considéré comme un des agents infectieux majeur affectant les élevages d’huîtres creuses, Crassostrea gigas, en France. Des différences de sensibilité à l’infection ont également été observées au sein de cette espèce. Des travaux précédents suggèrent un lien entre la base génétique et la survie des animaux face à l’infection. Dans ce contexte, l’objectif principal du travail de thèse était de mieux comprendre les interactions entre l’huître creuse et OsHV-1, et plus particulièrement, les bases moléculaires du cycle viral. Nos résultats montre que le virus est capable de se répliquer chez l’hôte quel que soit son stade de développement, et sa sensibilité. Cependant, la cinétique de multiplication est plus rapide chez des individus sensibles comparés aux moins sensibles. Il apparaît également que chez les individus survivants, le virus ne soit plus détectable après une phase de réplication active. Cette observation laisse suspecter (i) une rémission avec une élimination du virus ou (ii) une persistance du virus sans symptômes détectables. Ces résultats mettent en lumière la possibilité du virus de circuler au sein des individus survivants. Ces individus peuvent excréter des particules virales et intervenir ainsi dans le processus d’infection en milieu naturel. L’ensemble de ces résultats représentent une premier contribution à la compréhension du cycle d’ OsHV-1 chez l’huître creuse, plus particulièrement au niveau moléculaire
In France, Ostreid herpesvirus type 1 (OsHV-1), can be considered one of the major infectious agents in Pacific oysters, Crassostrea gigas. Susceptibility differences to infection were observed in this species. Previous work suggested that the genetic basis and the survival animals to infection were related. In this context, the main objective of this thesis was to understand the interactions between oysters and OsHV-1, in particular, the molecular basis of the viral cycle. Our results shows that the virus is able to replicate in the host regardless of its stage of development or its susceptible. However, multiplication kinetic is faster in susceptible individuals compared to less susceptible individuals. After a active replication, it would appear that the virus is no detectable in survival individuals. This observation suggests (i) a remission with elimination of the virus or (ii) a virus persistence without detectable symptoms. These results highlight the ability of the virus circulating in the host without causing mortality. These individuals can excrete viral particles and interfere with the infection process in field. All these results represent a first contribution to the understanding of OsHV-1 cycle in Pacific oysters, particularly at the molecular level

Phylum Mollusca is a large and diverse group of invertebrate protostomes of over 85,000 species including gastropods, cephalopods and bivalves. Molluscs are the largest marine phylum. Marine molluscs are economically important as a high protein food source for humans and provide ecosystem services including nutrient recycling, carbon sequestration, sediment stabilisation and bioturbation. Molluscs are harvested by traditional fishing but are increasingly cultured in many coastal communities worldwide. Hence most information on pathogens and disease is known in molluscs of commercial significance. Bivalves (oysters, mussels, clams, scallops, cockles) are susceptible to a wide range of diseases caused by viruses (e.g. ostreid herpes virus-1 and variants) bacteria (e.g. Vibrio spp., Nocardia crassostreae, Roseovarius spp., rickettsia and Mycobacterium spp.), microsporidians (e.g. Steinhausia spp.), paramyxids (Marteilia refringens and M. pararefringens), haplosporidans (e.g. Haplosporidium nelsoni, Minchinia spp., and Bonamia ostreae) and macroparasites (e.g. trematodes, copepods and nematodes). The gastropod abalone are susceptible to viruses (e.g. Abalone Viral Ganglioneuritis) and bacteria (Rickettsiales-like organism). Of particular importance is Pacific oyster mortality syndrome (POMS) that is polymicrobial in nature with initial infection by ostreid herpesvirus with subsequent bacterial infections by a variety of vibrios, and marteiliosis. In North America, since the early 1950’s there have been episodes of diseases including Dermo disease, caused by Perkinsus marinus, and MSX disease caused by H. nelson in Crassostrea spp. Globally, it is recognised that marine diseases including those that impact molluscs are becoming more frequent and severe due to climate change, in particular increasing seawater temperature, and human activities.