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sheikh, Umair. "Analysis of Power System Stability by Using Optimally Located SVC and STATCOM". Thesis, KTH, Elektriska energisystem, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-119248.
Pełny tekst źródłaAhmed, Sheikh. "PMU based PSS and SVC fuzzy controller design for angular stability analysis". Thesis, Kansas State University, 2015. http://hdl.handle.net/2097/20547.
Pełny tekst źródłaDepartment of Electrical and Computer Engineering
Shelli Starrett
Variability in power systems is increasing due to pushing the system to limits for economic purposes, the inclusion of new energy sources like wind turbines and photovoltaic, and the introduction of new types of loads such as electric vehicle chargers. In this new environment, system monitoring and control must keep pace to insure system stability and reliability on a wide area scale. Phasor measurement unit technology implementation is growing and can be used to provide input signals to new types of control. Fuzzy logic based power system stabilizer (PSS) controllers have also been shown effective in various studies. This thesis considers several choices of input signals, composed assuming phasor measurement availability, for fuzzy logic-based controllers. The purpose of the controller is to damp power systems’ low frequency oscillations. Nonlinear transient simulation results for a 4-machine two-area system and 50 machine system are used to compare the effects of input choice and controller type on damping of system oscillations. Reactive power in the system affects voltage, which in turn affects system damping and dynamic stability. System stability and damping can be enhanced by deploying SVC controllers properly. Different types of power system variables play critical role to damp power swings using SVC controller. A fuzzy logic based static var compensator (SVC) was used near a generator to damp these electromechanical oscillations using different PMU-acquired inputs. The goal was again improve dynamic stability and damping performance of the system at local and global level. Nonlinear simulations were run to compare the damping performance of different inputs on the 50 machine system.
Fellah, Clémentine. "Influence de la nature des interfaces carbonées au sein des composites SiC/SiC à renfort Hi-Nicalon S et Tyranno SA3 sur leur comportement mécanique". Thesis, Paris Sciences et Lettres (ComUE), 2017. http://www.theses.fr/2017PSLEM078.
Pełny tekst źródłaSiC/SiC composites including the third generation SiC fibers with pyrocarbon interphase (PyC) are promising candidates to improve the safety of nuclear reactors, especially for core materials such as cladding and to replace metallic alloys for these applications. Their intrinsic refractory properties, their neutron transparency and their microstructural stability when irradiated or exposed to high temperatures make them attractive for nuclear applications. However SiC fibers and SiC matrix are brittle ceramics. The integrity of the structures can be fulfilled only if the composite is damage tolerant and can acquire a pseudo-ductile mechanical behavior. An interphase is deposited between the fibers and the matrix to provide this damage tolerance of SiC/SiC composites.The ability of SiC/SiC composites to sustain damage is dictated by the fiber/matrix (F/M) coupling mode. The intensity of this coupling can be related to many parameters such as the roughness and the chemistry of the surface of the reinforcement. A carbon layer on the fiberssurface was highlighted by High Resolution Transmission Electronic Microscopy (HRTEM) and by physico-chemical analyses. The characteristics of this carbon layer vary with the fabrication process of the fibers. The impact of this carbon layer on the F/M coupling was investigated by the observation of the local damage mechanisms. To elucidate the local bonding modes governing the damage mechanisms at the F/M interface of these SiC/SiC composites, macroscopic mechanical tests have been coupled with observations of structural modifications occurring in the interface region after loading. Understanding the origin of this carbon layer allowed elucidating the local interaction mechanisms according to these studied materials. These mechanisms depend on the carbon structure of the SiC fibers surface which in turn governs the adhesion between this carbon and the PyC interphase. Thanks to this study, a surface treatment on fibers was developed to optimize the mechanical behavior of SiC/SiC composites, whatever the fibrous reinforcement chosen
Medová, Alena. "Analýza aplikace statistických metod v podniku AFL Stříbro. Porovnání s požadavky VDA". Master's thesis, Vysoká škola ekonomická v Praze, 2007. http://www.nusl.cz/ntk/nusl-3851.
Pełny tekst źródłaVaughn, Alexis. "How can we grow if we destroy our roots? An analysis of roots as metaphor for growth and urban change". Scripps College, 2010. http://ccdl.libraries.claremont.edu/u?/stc,88.
Pełny tekst źródłaVase, Ajoy. "The effects of material treatments on the surface properties of polymeric biomaterials". Pomona College, 2007. http://ccdl.libraries.claremont.edu/u?/stc,19.
Pełny tekst źródłaDiMarino, Christina Marie. "High Temperature Characterization and Analysis of Silicon Carbide (SiC) Power Semiconductor Transistors". Thesis, Virginia Tech, 2014. http://hdl.handle.net/10919/78116.
Pełny tekst źródłaMaster of Science
Rios, Mario Alberto. "Modélisation pour analyses dynamiques des réseaux électriques avec compensateurs de puissance réactive-SVC". Grenoble INPG, 1998. http://www.theses.fr/1998INPG0099.
Pełny tekst źródłaThis thesis studies the modeling and analysis of power System containing static VAR compensators (SVC). Low-order models, calculated by Hankel singular-value truncation réduction and N4Sid dynamic identification, provide the foundation for the modeling and analysis techniques. For cases where many SVCs or FACTS (Flexible AC Transmission Systems) devices are installed in a power System, a new subsystem structure based on thèse low-order modeling techniques has been developed. The proposed low-order models allow the computation of low-order LQG-type (Linear Quadratic Gaussian Regulator) control laws, as well as low-order robust controls, which provide a better performance relative to traditional SVC controls (proportional type). Using the subsystem structure formulated in this thesis, methods for calculating decentralized controllers for power Systems hâve been conceived. In addition, through the use of structured singular values, a new robustness analysis technique has been developed which allows the détermination of stability boundaries through the calculation of closed-loop transfer functions. The theoretical approaches in this work hâve been applied to a test power system containing 11 nodes, as well as a larger 173-node test system
Wong, Lilly. "Conformational analysis of C-terminal Src kinase /". Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2004. http://wwwlib.umi.com/cr/ucsd/fullcit?p3153687.
Pełny tekst źródłaTvedt, Valerie Maria. "Some factors that effect [sic] statistical power in ANCOVA: a population study". CSUSB ScholarWorks, 2000. https://scholarworks.lib.csusb.edu/etd-project/1764.
Pełny tekst źródłaRenkjumnong, Wasuta. "SVD and PCA in Image Processing". Digital Archive @ GSU, 2007. http://digitalarchive.gsu.edu/math_theses/31.
Pełny tekst źródłaFeng, Xiaohu. "SIC BASED SOLID STATE POWER CONTROLLER". UKnowledge, 2007. http://uknowledge.uky.edu/gradschool_diss/543.
Pełny tekst źródłaMISHRA, OM. "HUMAN MOTION ANALYSIS". Thesis, DELHI TECHNOLOGICAL UNIVERSITY, 2020. http://dspace.dtu.ac.in:8080/jspui/handle/repository/18772.
Pełny tekst źródłaMoos, Sarah. "Analyzing the interconnectedness between space, place, and human interaction with the natural environment: "Ecological reawakening: Organic DNA and evolution"". Scripps College, 2009. http://ccdl.libraries.claremont.edu/u?/stc,51.
Pełny tekst źródłaVase, Ajoy. "The effect of materials preparation on polymer surfaces". Pomona College, 2007. http://ccdl.libraries.claremont.edu/u?/stc,25.
Pełny tekst źródłaGuan, Wei. "New support vector machine formulations and algorithms with application to biomedical data analysis". Diss., Georgia Institute of Technology, 2011. http://hdl.handle.net/1853/41126.
Pełny tekst źródłaMi, Jian. "SiC Growth by Laser CVD and Process Analysis". Diss., Available online, Georgia Institute of Technology, 2006, 2006. http://etd.gatech.edu/theses/available/etd-04062006-135055/.
Pełny tekst źródłaLackey, W. Jack, Committee Chair ; Cochran, Joe K., Committee Member ; Danyluk, Steven, Committee Member ; Fedorov, Andrei G., Committee Member ; Rosen, David W., Committee Member ; Wang, Zhonglin, Committee Member.
Olivier, Ezra Jacobus. "Analysis of the extended defects in 3C-SiC". Thesis, Nelson Mandela Metropolitan University, 2008. http://hdl.handle.net/10948/730.
Pełny tekst źródłaMoghraby, Jeelan Salah. "Analysis of the human Spr18 SMC-like protein". Thesis, University of Sussex, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368280.
Pełny tekst źródłaBandara, Mikaila Jayaweera. "Analysis of the accessory Sec systems in streptococcus". Thesis, University of Bristol, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.688219.
Pełny tekst źródłaPandey, Vinayak. "Quasi-Static and Creep Behavior of Enhanced SIC/SIC Ceramic Matrix Composites". Thesis, Virginia Tech, 2000. http://hdl.handle.net/10919/33959.
Pełny tekst źródła
The creep data were also modeled using the representations such as Monkmon-Grant and Larsen-Miller equations. A modified Monkman-Grant equation was used to model the stratification of the creep strain rate data with temperature. A finite element model based on the plasticity theory was developed to simulate the quasi-static cyclic behavior of the material. Though the loading behavior of CMCs can be modeled using the bilinear or multilinear kinematic hardening plasticity models, the unloading behavior as predicted by the models is entirely different from the experimentally observed behavior. Hence, these models were modified to correctly predict the stress-strain behavior. The model, which was input via a user defined subroutine into the ANSYS finite element program uses the concept of state or internal variables to define the unloading portion of the stress-strain curve. The results were compared with the test data and they show very good agreement. The model was then used to predict the stress-strain response of a plate with a notch. The results from the analysis were compared with the experimental data and they show good agreement if average values of strains are considered.
Master of Science
Vaillancourt, Benoit. "Novel biophysical appliations [sic] of STICS". Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=111550.
Pełny tekst źródłaLarsson, Jonatan. "Automatic Test Generation and Mutation Analysis using UPPAAL SMC". Thesis, Mälardalens högskola, Akademin för innovation, design och teknik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-36415.
Pełny tekst źródłaWelham, Melanie J. "Functional and genetic analysis of the v-src oncogene". Thesis, University College London (University of London), 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.262020.
Pełny tekst źródłaLong, Andrew Edmund. "Cokriging, kernels, and the SVD: Toward better geostatistical analysis". Diss., The University of Arizona, 1994. http://hdl.handle.net/10150/186892.
Pełny tekst źródłaChen, Yang. "Damage mechanisms in SiC/SiC composite tubes : three-dimensional analysis coupling tomography imaging and numerical simulation". Thesis, Paris Est, 2017. http://www.theses.fr/2017PESC1056/document.
Pełny tekst źródłaBecause of their outstanding physical and chemical properties at high temperature, in comparison with metals, silicon carbide (SiC) composite materials are studied as possible nuclear fuel cladding materials either for future advanced fission/fusion reactors, or more recently, for the currently existing light water reactors. 2D-braided SiC/SiC composite tubes, manufactured by chemical vapor infiltration (CVI), exhibit an anisotropic, hardly deformable (~1%) mechanical behavior. Understanding the relations between the microstructure, the damage mechanisms and the macroscopic behavior is essential to optimize the structural design of this material for the considered applications. One important manufacturing parameter is the braiding angle, i.e. the angle between the fiber tows and the tube axis. The objective of this work is to provide a comprehensive understanding of the damage-microstructure relations, in particular of the effects of the braiding angle on the damage mechanisms. For this purpose, an investigation combining experimental observations at macro and micro-scale and numerical simulations is developed. The composite tubes are first studied through in situ tensile testing under X-ray computed tomography. Experiments were carried out on the PSICHE beamline at synchrotron SOLEIL using a pink polychromatic beam. The recorded 3D images are processed using the digital volume correlation (DVC) technique, extended by a series of advanced image processing algorithms specifically developed in order to analyze the 3D microstructures, to measure the deformations through the tube thickness, and to detect and quantitatively characterize the network of micro-cracks created by the mechanical loading. In addition, numerical simulations are performed on the real microstructures as observed in the high-resolution images recorded during the in situ tests. Stress fields are calculated at the microstructural scale in the elastic regime using a numerical tool based on the Fast Fourier Transform (FFT). They help to better understand crack initiation and interpret the experimental observations within one-to-one comparisons. Both the experimental and numerical approaches are applied to three tubes with different braiding angles (30°, 45° and 60°). The effect of the braiding angle on the initiation and evolution of damage in the bulk of the composite materials can thus be highlighted
Lei, Song. "Informative correlation extraction from and for Forex market analysis". AUT University, 2010. http://hdl.handle.net/10292/899.
Pełny tekst źródłaYip, Lai Ping Rhoda. "Comparing the effectiveness of differenet [sic] task types (information gap tasks and decision making tasks) on the promtotion [sic] of second language acquisition". HKBU Institutional Repository, 2004. http://repository.hkbu.edu.hk/etd_ra/557.
Pełny tekst źródłaMcDaniel, Caitlin Christine. "Madoff Madness: A Textual Analysis of the SEC's response to the Madoff Ponzi Scheme". Thesis, Virginia Tech, 2019. http://hdl.handle.net/10919/89486.
Pełny tekst źródłaMaster of Arts
On December 11, 2008, Bernard L. Madoff, of Bernard L. Madoff Investment Securities, LLC, was charged by the United States Securities and Exchange Commission (SEC) for his role in a $65 billion fraudulent scheme. During an investigation into Madoff’s business practices, it was revealed that the SEC overlooked many red flags that could have caught Madoff years earlier. As a result, the SEC faced reputational harm. This study examined the SEC’s crisis response to the public following the news of Madoff’s arrest by applying a crisis communication theory to discover “best practices” for government agencies when dealing with a preventable crisis. This study also offers suggestions to further expand crisis communication research and crisis response strategies in a government communication setting.
Carballo, Jose M. "Residual Stress Analysis in 3C-SiC Thin Films by Substrate Curvature Method". Scholar Commons, 2010. https://scholarcommons.usf.edu/etd/1590.
Pełny tekst źródłaBrown, Dennis W. "SPC toolbox : a MATLAB based software package for signal analysis /". Thesis, Monterey, Calif. : Springfield, Va. : Naval Postgraduate School ; Available from National Technical Information Service, 1995. http://handle.dtic.mil/100.2/ADA305260.
Pełny tekst źródła"September 1995." Thesis advisor(s): Monique P. Fargues. Includes bibliographical references. Also available online.
Zhang, Yixia, i 張義霞. "Refined non-conforming linear and nonlinear finte [sic] element analysis". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31242704.
Pełny tekst źródłaLee, Yiu-cheung, i 李耀祥. "Quatitative [sic] analysis of residential property value in Hong Kong". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1998. http://hub.hku.hk/bib/B3126900X.
Pełny tekst źródłaChan, Chunwa. "Design, simulation and analysis of RESURF Si/SiC power LDMOSFETs". Thesis, University of Warwick, 2018. http://wrap.warwick.ac.uk/102005/.
Pełny tekst źródłaFiorello, Michael D. "An analysis of the major pre-Siniatic [sic] worship narratives". Columbia, SC : Columbia Biblical Seminary and Graduate School of Missions, 2000. http://dx.doi.org/10.2986/tren.023-0154.
Pełny tekst źródłaZhang, Yixia. "Refined non-conforming linear and nonlinear finte [sic] element analysis". Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B22753060.
Pełny tekst źródłaLee, Yiu-cheung. "Quatitative [sic] analysis of residential property value in Hong Kong /". Hong Kong : University of Hong Kong, 1998. http://sunzi.lib.hku.hk/hkuto/record.jsp?B19872537.
Pełny tekst źródłaMacklin, Kendrick R. "Benchmarking and analysis of the SRC-6E reconfigurable computing system". Thesis, Monterey, Calif. : Springfield, Va. : Naval Postgraduate School ; Available from National Technical Information Service, 2003. http://library.nps.navy.mil/uhtbin/hyperion-image/03Dec%5FMacklin.pdf.
Pełny tekst źródłaLalime, Aimee L. "Development of a Computationally Efficient Binaural Simulation for the Analysis of Structural Acoustic Data". Thesis, Virginia Tech, 2002. http://hdl.handle.net/10919/34524.
Pełny tekst źródłaMaster of Science
VanSant, April Nelson. "Studies on Hydrogen-Pinch Analysis and Application of COSMO-SAC to Electrolytes". Thesis, Virginia Tech, 2008. http://hdl.handle.net/10919/34828.
Pełny tekst źródłaMaster of Science
Matras, Jan. "Aplikace reaktivních nanočástic do SAC pájecí pasty". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2018. http://www.nusl.cz/ntk/nusl-377074.
Pełny tekst źródłaZhang, Lianyang 1964. "Analyis [sic] and design of drilled shafts in rock". Thesis, Massachusetts Institute of Technology, 1999. http://hdl.handle.net/1721.1/9505.
Pełny tekst źródłaIncludes bibliographical references (p. 713-732).
Drilled shafts are widely used to transfer heavy structural loads (both axial and lateral) through the overburden soil to the underlying rock mass. It is important to consider the effect of discontinuities when designing drilled shafts in rock. This thesis develops (1) a new design method for axially loaded rock-socketed shafts; (b) new design methods for laterally loaded rock-socketed shafts; and (c) a method for assessing the three dimensional (30) geometric characteristics of discontinuities. The new design method for axially loaded rock-socketed shafts concentrates on the prediction of the ultimate side shear resistance and the end bearing capacity. New relations have been developed for both the side shear resistance and the end bearing capacity. Both developments are derived from the analysis of ex.tensive databases. For laterally loaded rock-socketed shafts, a simplified design method and a detailed design method were developed. The simplified design method assumes that the rock mass is isotropic and uses an elasto-plastic continuum model to predict the load-displacement response. This model can consider drilled shafts in rock overlain by soil and it was validated by comparing its results with available theoretical solutions and field test data. The detailed design method considers the effect of anisotropy of jointed rock masses. The key components of this method are the 30 geometric characterization of discontinuities and the estimation of the anisotropic defonnation and strength properties of jointed rock masses. The 30 characterization of discontinuities uses the observed trace data in a finite circular window (on an exposed outcrop or at the bottom of a shaft hole) to determine the corrected trace length distribution, the discontinuity size (diameter) distribution, the total number of discontinuities in an objective volume, and finally the fracture tensor which describes the intensity and orientation of all discontinuities in a rock mass. The method for estimating the anisotropic elastic deformation properties uses the superposhion-principle and adopts an equivalent continuum approach. Hill's criterion is used to describe the anisotropic yield/failure of jointed rock masses. The method was then applied to examine the effect of anisotropy on the lateral deformation of rock-socketed shafts. Also, design charts were developed for considering the effect of anisotropy when predicting the elastic defonnation of laterally loaded rock-socketed shafts. Finally, the applicability of the recommended design methods is discussed and a design example is presented.
by Lianyang Zhang.
Ph.D.
yin, jian. "High Temperature SiC Embedded Chip Module (ECM) with Double-sided Metallization Structure". Diss., Virginia Tech, 2005. http://hdl.handle.net/10919/30076.
Pełny tekst źródłaPh. D.
Soueidan, Maher. "Croissance hétéroépitaxiale du SiC-3C sur substrats SiC hexagonaux; Analyses par faisceaux d'ions accélérés des impuretés incorporées". Phd thesis, Université Claude Bernard - Lyon I, 2006. http://tel.archives-ouvertes.fr/tel-00136231.
Pełny tekst źródłaraison du désaccord de maille et de la dilatation thermique. Le SiC-3C peut aussi être déposé sur
substrats SiC-α(0001) en s'affranchissant des problèmes rencontré sur substrat Si. La difficulté de
contrôler la germination initiale génère cependant des macles qui sont difficiles à éviter ou éliminer
ensuite.
L'utilisation de l'épitaxie en phase vapeur comme technique de croissance n'a pas permis de
s'affranchir de ces macles malgré l'optimisation de la préparation de surface des germes SiC- α. En revanche, des couches de SiC-3C exemptes de macle ont été obtenues en utilisant une technique de
croissance originale, les mécanismes vapeur-liquid-solide, qui consiste à alimenter un bain Si-Ge avec
du propane.
La caractérisation des couches ainsi élaborées a montré une excellente qualité cristalline avec toutefois une incorporation non négligeable d'impuretés. Les éléments Al, Ge, B et Sn ont été dosés avec succès en utilisant des analyses par faisceaux d'ions accélérés, techniques peu conventionnelles pour SiC et présentant un challenge analytique.
Soueidan, Maher. "Croissance hétéroépitaxiale du SiC-3C sur substats SiC hexagonaux : analyses par faisceaux d'ions accélérés des impuretés incorporées". Lyon 1, 2006. http://tel.archives-ouvertes.fr/docs/00/13/62/31/PDF/these_soueidan.pdf.
Pełny tekst źródłaUsing silicon as substrate for growing 3C-SiC monocrystalline material generates too many defects in the layers due to lattice and thermal expansion mismatch. Though these difficulties are avoided by using hexagonal SiC substrates, the random formation of 60° rotated domains in the 3C layers generate a high density of twins. The use of vapour phase epitaxy for the growth did not allow reducing significantly the twin density despite the optimization of the in situ surface preparation of the seeds. On the other hand, these defects were eliminated by using Vapor-Liquid-Solid mechanism which consists in feeding a Si-Ge melt with propane. The characterization of these twin-free layers showed excellent crystalline quality. Some of the impurities incorporated during growth (Ge, Al, B, Sn) were successfully analysed using accelerated ion beam techniques though the detection and quantification of these elements inside SiC thin films are challenging
Das, Abhishek. "Analyses of crash occurence [sic] and inury [sic] severities on multi lane highways using machine learning algorithms". Orlando, Fla. : University of Central Florida, 2009. http://purl.fcla.edu/fcla/etd/CFE0002928.
Pełny tekst źródłaPerry, Kourtnie. "An analysis of race and gender portrayls [sic] on television commercials". Akron, OH : University of Akron, 2006. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=akron1163799784.
Pełny tekst źródła"December, 2006." Title from electronic thesis title page (viewed 10/15/2007) Advisor, Dudley B. Turner; Faculty readers, N. J. Brown, Kathleen D. Clark; Director, School of Communication, Carolyn Anderson ; Dean of the College, James Lynn; Dean of the Graduate School, George R. Newkome. Includes bibliographical references.
Char, Srinidhi Narasimha. "Transmission gate based logic for differential power analysis resistant circcuits [sic]". Connect to this title online, 2007. http://etd.lib.clemson.edu/documents/1193079255/.
Pełny tekst źródłaPatience, Trudy. "Sequence analysis of a Cowdria ruminantium lamdba (sic) GEM-11 clone". Thesis, Stellenbosch : Stellenbosch University, 2002. http://hdl.handle.net/10019.1/53052.
Pełny tekst źródłaENGLISH ABSTRACT: Heartwater is a major threat to livestock in Africa due to its high mortality rate. The intracellular nature of the causative organism, Cowdria ruminantium, makes it difficult to study, hence an effective and user-friendly vaccine has been extremely difficult to obtain. Two C. ruminantium DNA libraries have recently been constructed, the lambda GEM11 bacteriophage DNA library and the lambda ZAPII bacteriophage DNA library, and this has lead to a renewed search for protective genes that could be used as a vaccine against heartwater. In this study, several molecular techniques including PCR, cloning and sequencing were used to identify genes in the lambda GEM11 bacteriophage DNA library that code for proteins, which could be used as vaccines to protect susceptible animals against heartwater. The lambda GEM11 library was screened with a rickettsial secretory protein gene sequence, known as seeD. One positive colony was selected from which the bacteriophage DNA was isolated. The C. ruminantium DNA was amplified from the bacteriophage DNA by using PCR and C. ruminantium-specific primers. The C. ruminantium DNA was screened with Mycoplasma, bovine and Cowdria DNA probes. The amplified DNA was subeloned into two vectors and the clones were screened by restriction analysis to identify clones containing inserts. The appropriate clones were sequenced and overlapping sequences matched, ordered and aligned. Two sequences were continuous with a short sequence of unidentified bases in between. Oligonucleotide primers were designed to amplify the DNA sequence between the two contiguous sequences. This led to the identification of the entire sequence of the C. ruminantium genome contained within the bacteriophage plaque. The single contiguous sequence was analysed and the putative protein-coding sequences were obtained and compared to DNA sequences of known organisms using the BLAST program. Five open reading frames were identified with homology to genes encoding specific proteins in bacteria. Two open reading frames showed homology to the genes encoding the transporter proteins, FtsY and the ABC transporter, and three open reading frames were found to be homologous to genes encoding the essential enzymes dethiobiotin synthetase, pro lipoprotein diacylglycerol transferase and the putative NADH-ubiquinone oxidoreductase subunit. The five open reading frames encode for genes, which are essential for the normal functioning of the C. ruminantium organism. However, these open reading frames might not be effective for use in a DNA vaccine since none of the open reading frames showed homology to obvious genes that could play a role in immunity and therefore confer protection. The open reading frames can be used in mutagenesis studies to produce attenuated strains of the organism that possess mutated versions of these proteins. These attentuated strains could be used for the vaccination of cattle, and thereby confer protection against viable pathogenic C. ruminantium isolates.
AFRIKAANSE OPSOMMING: Hartwater is 'n bedreiging vir vee in Afrika weens die hoë mortaliteitssyfer verbonde aan die siekte. Die intrasellulêre aard van die organisme wat hartwater veroorsaak, Cowdria ruminantium, bemoeilik navorsing aangaande die organisme. Dit het tot gevolg dat 'n effektiewe en gebruikersvriendelike entstof moeilik bekombaar is. Daar is onlangs sukses behaal met die konstruksie van twee C. ruminantium DNA genoteke, die lambda GEM11 bakteriofaag genoteek en die lambda ZAPII bakteriofaag genoteek. Dit het gelei tot 'n herlewing in die soektog na beskermende gene, wat in 'n entstof teen hartwater gebruik kan word. In hierdie studie is verskeie molekulêre tegnieke insluitende PKR, klonering en geenopeenvolging bepaling, gebruik om gene te identifiseer in die lambda GEM11 bakteriofaag genoteek wat kodeer vir proteïene wat in entstowwe gebruik kan word as beskerming teen hartwater. Die secD geen is gebruik om die lambda GEM11 bakteriofaag genoteek te sif. Een positiewe plaak is gevind waarna die DNA uit die bakteriofaag plaak geïsoleer en die C. ruminantium DNA vanuit die bakteriofaag plaak geamplifiseer is deur gebruik te maak van PKR en spesifieke C. ruminantium inleiers. Die C. ruminantium DNA is gesif met Mycoplasma, bees en Cowdria radioaktief gemerkte DNA peilers. Die C. ruminantium DNA is vervolgens in twee vektore gekloneer. Die klone is gesif deur middel van restriksie analise. Die DNA volgorde van die klone is bepaal en twee ononderbroke sekwense is geïdentifiseer met 'n gaping in die middel tussen die twee sekwense. Oligonukleotied inleiers is daarna ontwerp om die geenopeenvolging van die gaping tussen die twee sekwense te vul. Hierdeur kon die volledige geenopeenvolging van die genoom van C. ruminantium wat in die lambda GEM 11 bakteriofaag plaak voorkom, bepaal word. Hierdie volledige geenopeenvolging is vervolgens geanaliseer en die oop leesrame wat daarin voorkom geïdentifiseer. Vyf leesrame is gevind om homologie met gene wat kodeer vir proteïene wat in bakterieë voorkom, te toon. Twee leesrame het homologie met die gene wat kodeer vir transport proteïene, FtsYen die ABC transporter getoon, en drie leesrame het homologie met gene wat kodeer vir die essensiële ensieme detiobiotin sintetase, prolipoproteïen diasielgliserol transferase en die NADHubikinoon oksidoreduktase subeenheid getoon. Dié vyf leesrame het die potensiaal om as entstowwe gebruik te word aangesien al vyf leesrame kodeer vir gene wat 'n belangrike rol speel in die oorlewing van die C. ruminantium organisme. Alhoewel die leesrame moontlik nie so effektief sal wees in 'n DNA entstof nie, toon dit potensiaal om in mutasieeksperimente gebruik te word. Organismes wat die gemuteerde weergawe van die geen besit sal nie-funksionele proteïene produseer, wat 'n invloed kan hê op die normale fisiologiese funksies van die organisme en dus sal lei tot 'n minder virulente organisme. Die geattenueerde organisme kan moontlik gebruik word om diere te immuniseer en daardeur immuniteit aan diere lewer wat beskerming sal bied teen patogeniese C. ruminantium isolate.
Waschura, Thomas E. "625 MBIT/SEC BIT ERROR LOCATION ANALYSIS FOR INSTRUMENTATION RECORDING APPLICATIONS". International Foundation for Telemetering, 1998. http://hdl.handle.net/10150/609650.
Pełny tekst źródłaThis paper describes techniques for error location analysis used in the design and testing of high-speed instrumentation data recording and communications applications. It focuses on the differences between common bit error rate testing and new error location analysis. Examples of techniques presented include separating bit and burst error components, studying probability of burst occurrences, looking at error free interval occurrence rates as well as auto-correlating error position. Each technique contributes to a better understanding of the underlying error phenomenon and enables higher-quality digital recording and communication. Specific applications in error correction coding emulation, magnetic media error mapping and systematic error interference are discussed.