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Quader, Sabina, i N/A. "Selective Synthetic Modification of Aminoglycosides for Drug Targeting to Tuberculosis". Griffith University. School of Biomolecular and Physical Sciences, 2007. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20071024.151619.
Pełny tekst źródłaQuader, Sabina. "Selective Synthetic Modification of Aminoglycosides for Drug Targeting to Tuberculosis". Thesis, Griffith University, 2007. http://hdl.handle.net/10072/367086.
Pełny tekst źródłaThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Biomolecular and Physical Sciences
Faculty of Science, Environment, Engineering and Technology
Full Text
Sherwin, Catherine M. T., i n/a. "Clinical pharmacology of aminoglycosides in neonates". University of Otago. Dunedin School of Medicine, 2009. http://adt.otago.ac.nz./public/adt-NZDU20090821.160736.
Pełny tekst źródłaBuranaphalin, Sawanya. "Pharmaceutical analysis of polyamines and aminoglycosides". Thesis, University of Bath, 2009. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.500693.
Pełny tekst źródłaRayet, Arjinder Kaur. "Analysis of the aminoglycosides neomycin and streptomycin". Thesis, Loughborough University, 1998. https://dspace.lboro.ac.uk/2134/26869.
Pełny tekst źródłaShrestha, Sanjib K. "Novel Aminoglycosides: Bioactive Properties and Mechanism of Action". DigitalCommons@USU, 2013. https://digitalcommons.usu.edu/etd/2073.
Pełny tekst źródłaXi, Hongjuan. "Thermodynaimic [sic] study of nucleic acids recognition by aminoglycosides". Connect to this title online, 2007. http://etd.lib.clemson.edu/documents/1202499410/.
Pełny tekst źródłaDahl, Russell Scott. "Synthesis of aminoglycosides and amino-C-glycosides from glycals /". Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2004. http://wwwlib.umi.com/cr/ucsd/fullcit?p3158462.
Pełny tekst źródłaPatwardhan, Anjali A. "Mechanistic studies of copper(II) aminoglycoside mediated DNA damage and magnesium catalyzed nuclease activity of hammerhead ribozyme". Connect to this title online, 2003. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1060971975.
Pełny tekst źródłaTitle from first page of PDF file. Document formatted into pages; contains xv, 91 p.; also includes graphics (some col.) Includes bibliographical references (p. 82-91). Available online via OhioLINK's ETD Center
Pinto, Nelson Schumacher John. "Pharmacokinetics of Amikacin after a single intravenous dose". Auburn, Ala., 2009. http://hdl.handle.net/10415/1848.
Pełny tekst źródłaSimon, Binto. "The development of synthetic methodology for stereoselective synthesis of aminoglycosides". Thesis, University of Manchester, 2013. https://www.research.manchester.ac.uk/portal/en/theses/the-development-of-synthetic-methodology-for-stereoselective-synthesis-of-aminoglycosides(746860dc-9b11-41b9-8283-9201d5149cd9).html.
Pełny tekst źródłaYatchang, Marina Fosso. "Synthesis and Biological Activity of Aminoglycosides and 1,4-Naphthoquinone Derivatives". DigitalCommons@USU, 2012. https://digitalcommons.usu.edu/etd/1371.
Pełny tekst źródłaZimmermann, Louis. "Synthèse et évaluation de dérivés amphiphiles de la néamine et de la néosamine, projet Néa et Néo". Thesis, Grenoble, 2012. http://www.theses.fr/2012GRENV084/document.
Pełny tekst źródłaIn regard to the antibacterial activity of amphiphilic aminoglycosides which are (i) 3',4',6-trinaphtylmethyl (2NM) et -trihexyl neamine derivatives active against wild-type and resistant to antibiotic drugs Gram (+) et Gram (–) bacteria, and (ii) 3',4'- and 3',6-di2NM derivatives active against bacteria Gram (+) bacteria, the works were focused on the search for more active and less toxic derivatives than the trialkyl derivatives. With this aim, two approaches were developed for obtaining antibacterial amphiphilic aminoglycosides: (1) the synthesis from neomycin B of new neamine derivatives carrying alkyl groups of various lipophily in order to decrease the global lipophily and (2) after the first results in the neamine series, the synthesis from N-acetylglucosamine of amphiphilic derivatives of the aminosugar part of the neamine core named neosamine.In the first approach, after optimisation of alkylation methods of neamine, we shifted from active 3',4',6-trialkylated derivatives to less lipophilic more active and less cytotoxic dialkylated derivatives, especially 3',6 derivatives. In the second approach, from 1-allyl 3,4-dinonyl neosamine, large spectrum antibacterial derivatives carrying at the anomeric position polar groups were obtained through epoxidation of the allylic double bond. Previously, the tri2NM neamine derivative has appeared to be able to strongly bind to the lipopolysaccharides of the outer membrane of P. aeruginosa and to destabilize membranes. The study of the mechanism of action of the most active derivatives prepared suggested a similar mode of action
Gremyachinskiy, Dmitriy. "Total synthesis of aminomethyl c-glycosides". Scholarly Commons, 2000. https://scholarlycommons.pacific.edu/uop_etds/544.
Pełny tekst źródłaChang, Jen-Chieh Jason. "The relative predictive performance of three pharmacokinetic programs for aminoglycosides dosing". Scholarly Commons, 1997. https://scholarlycommons.pacific.edu/uop_etds/2310.
Pełny tekst źródłaMcKay, Geoffrey A. "Characterization of aminoglycoside phosphotransferase APH(3')-IIIa : an enterococcal enzyme conferring resistance to aminoglycoside antibiotics /". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0034/NQ66223.pdf.
Pełny tekst źródłaJaunzems, Janis. "Solid-phase assisted synthesis of glycoconjugates, and synthesis of 15N-labelled aminoglycosides". [S.l. : s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=968659136.
Pełny tekst źródłaAszodi, Attila. "Études synthétiques dans le domaine des antibiotiques : les anthracyclines et les aminoglycosides". Paris 11, 1988. http://www.theses.fr/1988PA112160.
Pełny tekst źródłaTwo types of antibiotics were studied in this thesis: anthracyclines and aminoglycosides. The first chapter deals with anthracyclines in which the synthesis of new classes of anthracyclines, hopefully with reduced cardiotoxicity, were attempted. Two approaches were explored:1- Substitution of the benzylic group at position 7 by different alcoholates,2- DIELS-ALDER cycloaddition of a dienophile (epoxytetrone) to a diene bearing chiral cyclohexane substituant. This leads essantially in one step ta the tetracyclic skeleton with a good asymetrie induction. The second chapter deals with the preparation of 1,4-diaminocyclitol which based on a Ferrier rearrangement. The second amine is introduced by oximation follow by reduction
Akour, Amal. "The role of megalin in the transport of aminoglycosides across human placenta". VCU Scholars Compass, 2012. http://scholarscompass.vcu.edu/etd/448.
Pełny tekst źródłaAszodi, Bernadette. "Etudes synthétiques dans le domaine des antibiotiques les anthracyclines et les aminoglycosides /". Grenoble 2 : ANRT, 1988. http://catalogue.bnf.fr/ark:/12148/cb376114703.
Pełny tekst źródłaAlfindee, Madher N. "Synthesis and Biological Studies of Amphiphilic Compounds Derived from Saccharides and Aminoglycosides". DigitalCommons@USU, 2019. https://digitalcommons.usu.edu/etd/7568.
Pełny tekst źródłaMugabe, Clement, i Abdelwahab Omri. "La gentamicine sous la forme liposomale : aspects technologique et microbiologique". Acfas-Sudbury, 2005. https://zone.biblio.laurentian.ca/dspace/handle/10219/63.
Pełny tekst źródłaAlhariri, Moayad Abdulaziz I. "EVALUATION OF LIPOSOMAL BISMUTH-ETHANEDITHIOL-TOBRAMYCIN FOR TREATMENT OF CYSTIC FIBROSIS PULMONARY PSEUDOMONAS AERUGINOSA INFECTION". Thesis, Laurentian University of Sudbury, 2013. https://zone.biblio.laurentian.ca/dspace/handle/10219/2099.
Pełny tekst źródłaMcKee, Megan, Melanie McLeod i Laura Wicks. "Evaluation of Physicians’ Dosing Procedures for Obese Pediatric Populations and Pharmacokinetics of Aminoglycosides in these Patients". The University of Arizona, 2008. http://hdl.handle.net/10150/624298.
Pełny tekst źródłaObjectives: This was a retrospective chart review and survey of pediatric residents. This study aimed to examine standards for aminoglycosides in obese pediatrics; increase awareness of drug monitoring in obese populations; and reduce medication errors. Methods: 101 patients aged three to seventeen that received aminoglycoside treatment were included. Subjects were divided into three groups based on weight and height percentiles as defined by growth charts. Collecting retrospective data provided measured concentrations of aminoglycosides in order to evaluate pharmacokinetics. Data collected included: dose and frequency; time dose was given; length of infusion; two measured concentrations (peak and trough); and time concentration was measured. ANOVA allowed comparisons between aminoglycoside volumes of distribution to weight (based on specific weight groups). Tukey’s post hoc analysis further tested the significance of the pair-wise comparisons (p<0.05). Secondly, a questionnaire was administered to 26 pediatric medical residents at University Medical Center to assess current treatment protocols and attitudes towards medication dosing in obese pediatric patients. Results: The volume of distribution was not significantly different between normal weight and overweight patients (p=0.927); normal weight and obese patients (p=0.174); or overweight and obese patients (p=0.211). Most (81.8%) study participants have some difficulty finding references on dosing in overweight and obese patients. Conclusions: The positive correlation between volume of distribution and total body weight was not statistically significant. Pediatric residents agree that there is a lack of resources regarding obese pediatric medication dosing. Further research is warranted to ensure the reliability and validity of aminoglycoside dosing in obese children.
Mneimneh, Omar. "Evaluation of needs for pharmacokinetic monitoring of aminoglycosides and vancomycin in tertiary hospital". Master's thesis, Lithuanian Academic Libraries Network (LABT), 2007. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2007~D_20070803.122144-66772.
Pełny tekst źródłaToksiškų vaistų, tokių kaip aminoglikozidų ir vankomicino, bei racionalus vaistų vartojimo tendencijos Lietuvoje dar nėra pakankamai žinomos. Mūsų darbo tikslas buvo nustatyti ir įvertinti aminoglikozidų ir vankomicino suvartojimo KMU klinikose tendencijas , gentamicino ir vankomicino koncentracijas kraujo serume (KKS), bei įvertinti 17 stebėtų pacientų gydymo šiais antibiotikais atvejus. Vaistų suvartojimo duomenys buvo gauti iš ligoninės vaistinės ir jos administracijos. Apskaičiuotos DDD šimtui lovadienių per paskutinius trejus metus (2004 – 2006), nustatyti daugiausia 2006 metais aminoglikozidų ir vankomicino suvartoję KMU klinikų skyriai. Įvertintos 2006 m. antibiotikų KKS. 17 pacientų, gavę šiuos antibiotikus, buvo stebimi 7 mėnesius. Duomenų statistinė analizė buvo atlikta naudojant SPSS 16.0 statistinę duomenų apdorojimo programą, kokybinių duomenų įvertinimui atliktas Mann-Whitney testas neparametriniams kriterijams. Vidutinis gentamicino (240mg) suvartojimas 2004m. (±SE) DDD/100OBD buvo 3.67±0.69 (median 1.31; CI95% 2.29-5.06); 2005m. 4.53±1.87 (median 0.86; CI 95% 0.79-8.27); 2006m. 4.24±0.82 (median 1.05; CI95%2.60-5.88). Amikacino (1000mg) buvo 0.55±0.17 (median 1.22; CI95% 0.23-0.88) 2004m.; 2005m. 0.44±0.13 (median 1.03; CI 95% 0.18-0.69), ir 0.52±0.13 (median 1.08; CI95%0.27-0.78) 2006m. Tobramicino (240mg) buvo 0.03±0.02 (median 0.14; CI95% 0.00-0.07) 2004m, 0.006±0.003 (median 0.03; CI95% 0.00-0.01) 2005m. Vancomicino (2000mg) buvo 0.55±0.17... [toliau žr. visą tekstą]
Malott, Bradley. "Development and investigation of antibiotic resistance in E. coli using aminoglycosides". Wittenberg University Honors Theses / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=wuhonors1617617698561944.
Pełny tekst źródłaKawasaki, Yukie. "Aminoglycosides and Syringomycin E as Fungicides Against Fusarium graminearum in Head Blight Disease". DigitalCommons@USU, 2008. https://digitalcommons.usu.edu/etd/202.
Pełny tekst źródłaLang, Manon. "Un nouveau mécanisme d’entrée des aminosides dans les bactéries Gram-négative". Electronic Thesis or Diss., Sorbonne université, 2023. http://www.theses.fr/2023SORUS143.
Pełny tekst źródłaThe number of deaths due to multidrug-resistant bacteria was over one million in 2019, demonstrating that the fight against pathogenic bacteria is a major public health issue. Aminoglycosides (AGs) are broad-spectrum antibiotics, effective against Gram-negative bacteria due to their ability to cross the double membrane barrier using the proton motive force, but the precise mechanism remains to be elucidated. Our laboratory has discovered a novel mechanism of entry of AGs into V. cholerae via sugar transporters. In this study, we showed that overexpression in Escherichia coli of multiple carbohydrate transporters increased sensitivity to AGs while deletion of a single transporter had little impact on sensitivity, suggesting a redundancy of these transporters capable of compensating for each other and limiting the development of resistance. Using a "proof-of-concept" transporter called CmtA, we confirmed that differential entry of AGs was linked to the deletion or overexpression of this transporter. This mechanism is shared with other pathogens, since the same sensitivity to AGs was observed upon overexpression of sugar transporters in Pseudomonas aeruginosa. Increasing the production of these transporters in response to the presence of a substrate in vivo could allow for greater efficacy and lower side effects of AG therapies by allowing better uptake by bacteria. We therefore sought a substrate capable of increasing the expression of transporters involved in AG uptake, and identified the ribonucleoside uridine as an activator. The addition of uridine to the culture medium allows for greater AG uptake in E. coli and did not show a mutant appearance. By mimicking a urinary tract infection with a synthetic medium, the addition of uridine potentiates AG treatment by decreasing the minimum inhibitory concentration of AGs and accelerating death kinetics. We propose uridine as a potentiator of GA treatment by co-administering an AG with uridine to stimulate its entry. This study paves the way for improving these treatments by using carbohydrates to stimulate AGs molecules uptake
Hon, Wai-Ching. "Crystallographic studies on two structures of a kanamycin kinase : a Mg-AMPPNP and a Mg-ADP complex /". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0001/NQ42744.pdf.
Pełny tekst źródłaLevy, Jack. "Etude de la résistance aux aminoglycosides chez haemophilus influenzae: incidence, base génétique et mécanisme". Doctoral thesis, Universite Libre de Bruxelles, 1985. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/213662.
Pełny tekst źródłaZhang, Qian. "Synthesis of Flouronogenic Probes for Studying Biomass Degradation and Synthesis of New Antifungal Aminoglycosides". DigitalCommons@USU, 2015. https://digitalcommons.usu.edu/etd/4472.
Pełny tekst źródłaGuyon, Hélène. "Mise au point d'une méthode de mesure d'interaction ligand-ARN par électrochimie". Thesis, Sorbonne Paris Cité, 2016. http://www.theses.fr/2016USPCB147/document.
Pełny tekst źródłaRNA molecules play a major role in various biochemical processes and they are now considered as an important drug target. However, our limited understanding of the interactions occurring between small molecules and RNA complicate the search for new ligands (or drugs) with improved specific interaction and binding to elaborated RNA structures. In the absence of any rational approach, a screening strategy could shed light on the ligand/RNA interactions. In this thesis, we describe a simple electrochemical approach allowing for high-throughput detection and quantification of small molecule/RNA interactions. The principle of the method relies on the difference of diffusion rates between a redoxmolecular probe free or bound to its RNA target and thus to the ability to more easily electrochemically detect the forme rover the latter in a homogenous solution. This electrochemical detection strategy has the advantages of being affordable,fast, easy to use, sensitive and well-adapted to a high-throughput screening strategy in small volume samples. This methodology was used to characterize the binding of an aminoglycoside analog bearing a ferocenyl group to the ribosomal RNA fragment (rRNA 16S23). Furthermore, competitive binding of unlabelled aminoglycosides on theRNA/electrochemical probe complex allowed us to evaluate their dissociation constants (KD). These competitive experiments could further be generalized to measure KD values for libraries of molecules, which could help to find better RNA ligands
Castegren, Markus. "Modulating Organ Dysfunction in Experimental Septic Shock : Effects of Aminoglycosides, Antiendotoxin Measures and Endotoxin Tolerance". Doctoral thesis, Uppsala universitet, Infektionssjukdomar, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-149274.
Pełny tekst źródłaPaper 3, previous title as submitted: "Compartmentalization of organ endotoxin tolerance in a porcine model of secondary sepsis"
François, Boris. "Cristallographie de complexes entre site de décodage ribosomique et antibiotiques de la famille des aminoglycosides". Université Louis Pasteur (Strasbourg) (1971-2008), 2005. https://publication-theses.unistra.fr/public/theses_doctorat/2005/FRANCOIS_Boris_2005.pdf.
Pełny tekst źródłaBolard, Arnaud. "Identification of novel regulatory pathways involved in non-enzymatic resistance to aminoglycosides in Pseudomonas aeruginosa". Thesis, Bourgogne Franche-Comté, 2019. http://www.theses.fr/2019UBFCE006/document.
Pełny tekst źródłaAntibiotics are invaluable drugs to combat bacterial infections. Emergence and spread of antibiotic resistance in the opportunistic pathogen Pseudomonas aeruginosa have led the World Health Organization to consider as a crucial priority the development of new therapeutic approaches to fight this bacterium. In addition to other alternatives, preservation of activity of major antibiotics such as aminoglycosides and colistin is primordial. Consequently, characterization of the resistance mechanisms to these drugs is a prerequisite to design novel molecules, and improve patient care. In this context, we show that mutations in gene fusA1 (encoding elongation factor EF-G1A) and in operon pmrAB (two-component system PmrAB) lead to an increased resistance to aminoglycosides in in vitro-selected mutants and strains isolated from cystic fibrosis (CF) and non-CF patients. Certain amino acid substitutions in EF-G1A confer a 2- to 16-fold increased resistance to the four aminoglycoside subclasses. On the other hand, amino acid variations in two-component system PmrAB activate the expression of genes PA4773-PA4774-PA4775, and production of norspermidine and spermidine. This upregulated polyamine biosynthesis is associated with a 4- to 16-fold decreased susceptibility to 4,6-di-substituted deoxystreptamine aminoglycosides (gentamicin, amikacin and tobramycin). Moreover, our work reveals that the acquired resistance of pmrB mutants to colistin partially depends upon pump MexXY(OprM), a system that otherwise mediates intrinsic, adaptive and acquired resistance to aminoglycosides. Finally, we show that pmrB mutants overproduce azetidine-containing alkaloids by a quorum-sensing-regulated, nonribosomal peptide synthetase pathway. These alkaloids impair the virulence of P. aeruginosa in a Galleria mellonella infection model
Bugaud, Olivier. "Suppression traductionnelle des codons stop chez les mammifères". Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLS222.
Pełny tekst źródłaNonsense mutations, also known as premature termination codons (PTCs) are responsible for 10% to 30% of all human genetic diseases. Nonsense translation suppression can be induced by readthrough inducers. The presence of such PTC leads to premature translation termination. These stop therapeutic strategies have emerged which attempt to use molecules that facilitate tRNA incorporation at the PTC (readthrough). The, translation continue in the same reading frame until the next stop codon. I first developed an innovative screening system I used to test more than 17,000 molecules and have identified one hit, TLN468 molecule. I have shown that this molecule is able to induce re-expression of an active p53 protein.I also characterized new compounds derived from aminoglycosides. I have shown that the NB124 induces apoptosis of tumor cells by re-expressing p53 protein while having a much lower toxicity than gentamicin.I developed a single molecule approach for studying the ribosome programmed errors (recoding). I was able to analyze the kinetics of elongation eukaryotic ribosomes and showed that the initiation of translation at an internal entry site (IRES) slows the ribosome during the first elongation cycle
BUGNON, DENIS. "Methode de simulation de la pharmacocinetique humaine chez l'animal : nouvelle technique et son application aux aminoglycosides". Nantes, 1991. http://www.theses.fr/1991NANT120M.
Pełny tekst źródłaRudolph-Claasen, Zerilda Suzette. "Hearing loss amongst DR-TB patients that have received extended high-frequency pure tone audiometry monitoring (KUDUwave) at three DR-TB decentralized sites in Kwazulu Natal". University of the Western Cape, 2018. http://hdl.handle.net/11394/6583.
Pełny tekst źródłaOtotoxic induced hearing loss is a common adverse event related to aminoglycosides used in Multi Drug Resistant -Tuberculosis treatment. Exposure to ototoxic drugs damages the structures of the inner ear. Symptomatic hearing loss presents as tinnitus, decreased hearing, a blocked sensation, difficulty understanding speech, and perception of fluctuating hearing, dizziness and hyperacusis/recruitment. The World Health Organization (1995) indicated that most cases of ototoxic hearing loss globally could be attributed to treatment with aminoglycosides. The aim of the study was to determine the proportion of DR-TB patients initiated on treatment at three decentralized sites during a defined period (1st October to 31st December 2015) who developed ototoxic induced hearing loss and the corresponding risk factors, whilst receiving audiological monitoring with an extended high frequency audiometer (KUDUwave). A retrospective cross-sectional study was conducted. Cumulatively across the three decentralized sites, 69 patient records were reviewed that met the inclusion criteria of the study. The mean age of the patients was 36.1, with a standard deviation (SD) of 10.7 years; more than half (37) were female. Ototoxicity , a threshold shift, placing patients at risk of developing a hearing loss was detected in 56.5% (n=39)of patients and not detected in 30.4%(n=21).The remaining 13,1% (n=9)is missing data. As a result, the regimen was adjusted in 36.2% of patients. . From the 53 patients who were tested for hearing loss post completion of the injectable phase of treatment, 22.6% (n=12) had normal hearing, 17.0 % (n=9) had unilateral hearing loss, and 60.4% (n=32) had bilateral hearing loss. Therefore, a total of 41 patients had a degree of hearing loss: over 30% (n=22)had mild to moderate hearing loss, and only about 15% (n=11)had severe to profound hearing loss. Analysis of risk factors showed that having ototoxicity detected and not adjusting regimen significantly increases the risk of patients developing a hearing loss. The key findings of the study have shown that a significant proportion of DR-TB patients receiving an aminoglycoside based regimen are at risk of developing ototoxic induced hearing loss, despite receiving audiological monitoring with an extended high frequency audiometer that allows for early detection of ototoxicity (threshold shift).
Hunt, Kevan Owen. "An epidemiological study in the greater Durban area of gram negative bacilli resistant to aminoglycoside antibiotics". Thesis, Cape Technikon, 1998. http://hdl.handle.net/20.500.11838/2254.
Pełny tekst źródłaThis study was undertaken to investigate resistance to aminoglycoside antibiotics and the transfer of resistance in selected Gram negative bacilli in hospitals in the Greater Durban area in order to determine whether the development of resistance in this region was similar to that found in other countries and whether it was the same in the hospitals in the region. It was intended that the study might expose the existence of nosocomial pathogens of a particular strain or endemic plasmids responsible for aminoglycoside antibiotic resistance. Strains of Klebsiella, Enterobacter and Serratia species and Escherichia coli resistant to gentamicin, tobramycin, netilmicin or amikacin were obtained. Resistance of the isolates obtained to the above aminoglycoside antibiotics was confirmed using a disc diffusion technique. Resistance mechanisms were initially assigned on the basis of resistance to these four aminoglycoside antibiotics. In approximately 50% of the isolates, including donor isolates and their respective transconjugants, resistance mechanisms were confirmed or revised on the basis of a changed resistance profile to a range of 12 aminoglycoside antibiotics in conjunction with DNA/DNA hybridization tests. Bacterial conjugation studies were performed on selected isolates to investigate the transfer of aminoglycoside resistance from Klebsiella pneumoniae isolates to recipient Escherichia coli. Plasmid profiles of all isolates and Escherichia colitransconjugants were compared to establish similarities. Isolates in three of the four genera of bacteria and all isolates collectively, demonstrated the greatest incidence of resistance to tobramycin. Amikacin resistance was, in all groups of isolates, the least frequently encountered. Collectively, the most frequent mechanisms of resistance were the AAC(3)-V and AAC(6')-1 enzymes One large hospital showed a high frequency of the AAC(3)-V modifying enzyme while in other hospitals a wider range of enzyme resistance mechanisms were evident. Plasmid profiles were generally dissimilar within and between different genera and the different hospitals.
Mangosuthu Technikon Research Fund
Geyer, Lúcia Bencke. "Limiares auditivos em altas frequências e emissões otoacústicas em pacientes com fibrose cística". reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2014. http://hdl.handle.net/10183/98476.
Pełny tekst źródłaIntroduction: the treatment of patients with cystic fibrosis involves the use of ototoxic drugs, and the most frequently used are the aminoglycoside antibiotics. Due to the frequent use of this drug, cystic fibrosis patients are at risk to develop hearing loss. Objective: the aim of this study was to evaluate the hearing of patients with cystic fibrosis by high frequency audiometry (HFA) and distortion product otoacoustic emissions (DPOAE). Patients and methods: retrospective and prospective crosssectional study including 75 individuals, 39 of the study group and 36 in the control group. HFA (250 – 16,000 Hz) and DPOAE tests were conducted. Results: the study group had thresholds significantly higher in the HFA in 250, 1,000, 8,000, 9,000, 10,000, 12,500 and 16,000 Hz (p=0.004) and higher prevalence of abnormal DPOAE at 1,000 and 6,000 Hz (p=0.001), with significantly lower amplitudes of 1,000, 1,400 and 6,000 Hz. There was a significant association between changes in hearing thresholds in HFA with the number of courses of aminoglycosides performed (p=0.005). Eighty-three percent of patients who completed more than 10 courses of aminoglycosides had hearing loss in HFA. Conclusion: a significant number of patients with cystic fibrosis who received repeated courses of aminoglycosides showed alterations in HFA and DPOAE.
Pothayee, Nikorn. "Development of Core-Shell Polymeric Nanostructures for Delivery of Diagnostic and Chemotherapeutic Agents". Diss., Virginia Tech, 2010. http://hdl.handle.net/10919/77286.
Pełny tekst źródłaPh. D.
Heffernan, Aaron J. "Dose optimisation of intravenous and nebulised antibiotics for the treatment of Pseudomonas aeruginosa pneumonia". Thesis, Griffith University, 2023. http://hdl.handle.net/10072/421611.
Pełny tekst źródłaThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Medicine & Dentistry
Griffith Health
Full Text
Lombès, Thomas. "Synthèse d’analogues d’aminoglycosides par voie chimique et ingénierie métabolique : Application à l’étude des ARN par RMN du fluor". Thesis, Paris 5, 2012. http://www.theses.fr/2012PA05T044/document.
Pełny tekst źródłaPas de résumé en anglais
RUZAFA, ISABELLE. "Aminoglycosides en pathologie infectieuse respiratoire severe : etude de l'efficacite et de la tolerance de l'amikacine en mono-dose quotidienne chez 27 patients". Toulouse 3, 1992. http://www.theses.fr/1992TOU31118.
Pełny tekst źródłaSOUZA, CABRINI FERRAZ DE. "DEVELOPMENT OF CAPILLARY ELECTROPHORESIS BASED METHODS WITH DIFFERENT DETECTION APPROACHES FOR DETERMINATION OF ORGANOTINS, STROBILURINS AND AMINOGLYCOSIDES". PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2013. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=23181@1.
Pełny tekst źródłaCOORDENAÇÃO DE APERFEIÇOAMENTO DO PESSOAL DE ENSINO SUPERIOR
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO
PROGRAMA DE EXCELENCIA ACADEMICA
Neste trabalho, métodos baseados em diferentes abordagens em eletroforese capilar (CE) foram propostos. No caso da determinação de compostos organoestanhos ou OTs (difenilestanho e monofenilestanho) em fluidos biológicos, foi usada abordagem de eletroforese capilar por zona (CZE) hifenada com a espectrometria de massas (do tipo quadrupolo) com fonte de plasma indutivamente acoplado (CE-ICP-MS). As condições de análise foram estudadas no modo univariado visando otimizar a composição da solução eletrolítica (tampão acetato 5,0 mmol L(-1), pH 2,8) e obter os parâmetros instrumentais (45 graus Celsius, mais 30 kV e 30 s de tempo de introdução hidrodinâmica de amostra). A solução de complementação foi uma solução aquosa 5,0 mmol L(-1) de NH4NO3 contendo 10 por cento metanol em volume e 1,0 Mg L(-1) de Cspositivo, com pH ajustado para 2,8 com tampão acetato. A vazão dessa solução foi mantida em 40 ML min(-1). Os OTs foram diluídos em solução de metanol:tampão acetato de sódio 50:50 por cento v/v ou apenas em tampão acetato de sódio pH 2,8. As condições de detecção do ICP-MS foram ajustadas em 1200 W, 15 L min(-1) de vazão de argônio para formação do plasma, 1 L min(-1) de vazão de argônio auxiliar. A vazão de argônio do nebulizador foi ajustada diariamente. Os isótopos de estanho 120Sn e 118Sn foram monitorados, assim como o 133Cspositivo para controlar a eficiência e estabilidade do processo de nebulização. A resposta linear do método ficou entre 0,050 a 2,0 mg L(-1) de Sn (0,42 a 17 Mmol L-(1)). Os limites de detecção (LOD) e de quantificação (LOQ) em termos de Sn foram de 15 Mg L(-1) (0,13 Mmol L(-1)) e 50 Mg L-1 (0,42 Mmol L(-1)), calculados utilizando a menor concentração dos picos dos analitos que podem ser diferenciados do sinal de fundo. A repetibilidade para o tempo de migração e área dos picos ficou próximo a 5 por cento. O método foi aplicado na análise de urina, sangue total e plasma fortificados com os OTs. Recuperações entre 75 e 95 por cento foram obtidas. No caso da determinação de sete pesticidas da classe das estrobilurinas (azoxistrobina, 9 dimoxistrobina, fluoxastrobina, picoxistrobina, piraclostrobina, trifloxistrobina e kresoxim-metil) em sopas infantis, foi usada a cromatografia eletrocinética capilar micelar (MEKC) com detecção fotométrica (no UV) com capilar de caminho óptico estendido. Um estudo multivariado, usando um planejamento 33 Box Behnken, indicou que a melhor separação para os pesticidas foi com solução aquosa de eletrólito composto por tampão tetraborato de sódio (5,1 mmol L(-1), pH 9,0) contendo 51 mmol L(-1) de dodecil sulfato de sódio (SDS) e 24 por cento acetonitrila (ACN) em volume. As condições instrumentais foram 25 C e mais 30 kV de diferença de potencial aplicada, 45 s de tempo de introdução hidrodinâmica de amostra e detecção em 210 nm. Para aumentar o poder de detecção, foi usada a concentração dos analitos no capilar. Para tal, as soluções de padrões e amostras foram dissolvidas em solução tampão tetraborato de sódio 45 mmol L(-1): acetonitrila 80:20 por cento v/v. As curvas analíticas apresentaram comportamento linear e os valores de LOD ficaram entre 7,0 Mg L(-1) ou 18 nmol L(-1) (piraclostrobina) a 15 Mg L-1 ou 33 nmol L(-1) (fluoxastrobina). Os valores de LOQ ficaram entre 21 Mg L(-1) ou 54 nmol L(-1) (piraclostrobina) a 45 Mg L(-1) ou 98 nmol L(-1) (fluoxastrobina). A repetibilidade ficou entre 1,7 a 7,9 por cento para a área de pico e entre 0,25 a 0,71 por cento para o tempo de migração. A precisão intermediária, avaliada com análises realizadas em diferentes dias, apresentou valores entre 1,3 a 5,3 por cento para a área de pico e entre 0,06 a 0,90 por cento para o tempo de migração. O método foi aplicado na análise de sopas prontas infantis fortificadas com as estrobilurinas. Os pesticidas foram extraídos aplicando o método QuEChERS com ajuste de pH com tampão acetato e limpeza com extração em fase sólida dispersiva. Os resultados das análises obtidos c
In this work, analytical methods based on different approaches using capillary electrophoresis (CE) have been proposed. For the determination of organotins or OTs (diphenyltin and monophenyltin) in biological fluids, the separation using capillary zone electrophoresis (CZE) was applied using tandem with inductively coupled plasma mass spectrometry (CE-ICP-MS). The conditions for the analysis were optimized in an unvaried way aiming to find the conditions for the electrolyte solution (acetate buffer, 5.0 mmol L (-1), pH 2.8) and the employed instrumental parameters (45C, 30 kV and 30 s of the time for hydrodynamic introduction of the sample). A complementary solution was composed by NH4NO3 5.0 mmol L(-1), 10 por cento v/v of methanol and 1.0 g L(-1) of Cspositive in acetate buffer with pH adjusted to 2.8. The flow of this solution was set to 40 ML min(-1). The OTs were diluted either in a methanol: acetate buffer 50:50 por cento v/v solution or only in sodium acetate buffer pH 2.8. The conditions for detection by ICP-MS were set to 1200 W, 15 L min(-1) for the Ar plasma flow and 1,0 L min(-1) for the auxiliary Ar. The nebulizer Ar flow was adjusted daily. The monitored tin isotopes were 120Sn 118Sn. The isotope 133Cs was also monitored in order to control the efficiency and stability of the nebulization. The method presented a linear response between 0.05 and 2.0 mg L(-1) (0.42 a 17 Mmol L(-1)) for Sn. The value for the limits of detection (LOD) and for the limits of quantification (LOQ) for Sn were 15 Mg L(-1) (0.13 Mmol L-1) e 50 Mg L(-1) (0.42 Mmol L(-1)), calculated based on the lowest concentration of the analyte peaks that can be differentiated from the background signal. The repeatability for migration time and peak area was approximately 5 per cent. The method was applied in the analysis of organotin fortified blood and urine samples with recoveries between 75 and 95 per cent. In the case of the determination of seven strobilurin class pesticides (azoxystrobin, dimoxystrobin, fluoxastrobin, picoxystrobin, pyraclostrobin, trifloxystrobin and kresoxim-methyl) in baby food (vegetable and fruit soups), 12 the micellar electrokinetic capillary chromatography (MEKC) was used using photometric detection (UV) in a capillary with extended optical path. A multivariate study, with 33 Box Behnken design, indicated the best composition for the electrolytic solution to separate the seven pesticides: a sodium tetraborate buffer (5.1 mmol L(-1), pH 9.0) solution containing 51 mmol L(-1) sodium dodecyl sulfate and acetonitrile (24 por cento in volume). The instrumental conditions were 25C, 30 kV of applied voltage, 45 s for hydrodynamic introduction of the sample and detection at 210 nm. To increase the detection power, the concentration of the analytes into the capillary was used by using the Normal Stacking Mode. For this purpose, the solutions of standards and samples were prepared in 45 mmol L(-1) sodium tetraborate buffer solution: acetonitrile 80:20 por cento v/v. The analytical curves presented a linear behavior and the LOD values were between 7.0 Mg L (-1) or 18 nmol L(-1) (pyraclostrobin) to 15 Mg L(-1) or 33 nmol L(-1) (fluoxastrobin). The LOQ values were between 21 Mg L(-1) or 54 nmol L(-1) (pyraclostrobin) a 45 Mg L(-1) ou 98 nmol L(-1) (fluoxastrobin). The repeatability was between 1.7 to 7.9 por cento for the peak area and between 0.25 to 0.71 por cento for the migration time. The intermediate precision, evaluated by the analysis performed in different days were between 1.3 to 5.3 por cento for the peak area and between 0.06 and 0.90 per cent for the migration time. The method was applied in the analysis of baby food spiked with strobilurin. Pesticides were extracted using the QuEChERS method with pH adjustment with acetate buffer and clean-up using the dispersive solid phase extraction. The analysis results were statistically identical to those obtained with a chromatographic method adapted from the literature. The CZE separation mode was chosen to evaluate the potential of the indirect determination of ami
Pradier, Léa. "Une approche éco-évolutive de la propagation de la résistance antibiotique : l'exemple de la résistance aux aminoglycosides". Thesis, Université de Montpellier (2022-….), 2022. http://www.theses.fr/2022UMONG014.
Pełny tekst źródłaIn front of the antibiotic pressure, a wide range of resistance mechanisms exist. They represent an increasing public health issue, which does and will have major consequences on human mortality. Current strategies —mostly based on antibiotic consumption reductions— globally fail to contain this persistent issue. Moreover, even though it is undeniable that the extensive antibiotic use during the past decades has been a driver of antibiotic resistance evolution, its impact on the emergence and the propagation of antibiotic resistance is often overestimated compared to other factors. First, selection on antibiotic resistance cannot be restricted to the medical and veterinary uses of antibiotics. Second, the evolution (i.e. the emergence, maintenance and spread) of a trait in populations also relies on other evolutionary forces and ecological processes. Finally, the intertwining of these factors induces an intricate evolution of resistant phenotypes, relying on several scales ranging from genetic determinism to environmental dynamics. The aim of this thesis is therefore, through an eco-evolutionary approach, to improve the understanding of forces and mechanisms shaping directions and movements of antibiotic resistance genes. To do so, this thesis focuses on aminoglycoside resistance by modifying enzymes. The results of this work highlight the importance of ecological and genomic factors in the circulation of resistance genes, and thus conclude that integrative studies of resistance are thus required. To begin with, this thesis reveals that the distribution of aminoglycoside resistance is loosely linked to variation in aminoglycoside consumption across Europe, but strongly structured by globalization (trade, migration) and even more by ecological connectivity. Subsequent analysis underscores the underestimated role of non-pathogenic bacteria in the horizontal transfer of antibiotic resistance genes at large phylogenetic scales. In the light of these results, this thesis opens up a course of reflection on the study and management of antibiotic resistance
Subedi, Yagya P. "Cost-Effective Synthesis, Bioactivity and Cellular Uptake Study of Aminoglycosides with Antimicrobial and Connexin Hemichannel Inhibitory Activity". DigitalCommons@USU, 2019. https://digitalcommons.usu.edu/etd/7699.
Pełny tekst źródłaFindlay, Brandon. "Design and synthesis of cationic amphiphiles". American Society for Microbiology, 2010. http://hdl.handle.net/1993/21708.
Pełny tekst źródłaGremyachinskiy, Dmitriy. "Total synthesis of aminomethyl c-glycosides : a thesis". Scholarly Commons, 2001. https://scholarlycommons.pacific.edu/uop_etds/544.
Pełny tekst źródłaBender, Eduardo André. "Caracterização fenotípica e genotípica de amostras de enterococcus spp. isoladas em dois hospitais de Porto Alegre". reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2008. http://hdl.handle.net/10183/12599.
Pełny tekst źródłaThe phenotypic and genotypic characteristics of 203 Enterococcus spp isolates recovered from different clinical sources of two hospitals in Porto Alegre, Rio Grande do Sul, Brazil, were studied. The species were identified by conventional biochemical tests and the automated system VITEK 2 (BioMérieux). The minimal inhibitory concentrations (MIC) for aminoglycosides were evaluated by agar dilution method. The evaluation of high-level resistance to aminoglycosides (HLAR) and resistance to ampicillin were carried out by the same method as well as by the disc-diffusion method. The genetic diversity of HLAR E. faecalis was assessed by pulsed-field gel electrophoresis of cromossomal DNA after SmaI digestion. The E. faecalis was the most frequent specie (93.6%), followed by E. faecium (4.4%). The percentage of resistance among the clinical isolates was: 2.5% to ampicillin, 0.5% to vancomycin, 0.5% teicoplanin, 33% to chloramphenicol, 2% to nitrofurantoin, 66.1% to erythromycin, 66.5% to tetracycline, 24.6% to rifampicin, 30% to ciprofloxacin and 87.2% to quinupristin-dalfopristin. A total of 10.3% proved to be HLAR, with 23.6% resistant to gentamicin and 37.4% to streptomycin. Most of the aminoglycosides susceptible isolates by the disc-diffusion method presented MIC lower than 125 μg/mL and 500μg/mL for gentamicin e streptomycin, respectively. The prevalence of vancomycin resistance Enterococcus (VRE) was very low in this study. One predominant clonal group was found among E. faecalis exhibiting HLR-Ge/St. The isolates included in the clonal group were recovered from both hospitals, indicating both intrahospital and interhospital spread of this clone.