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Klebensberger, Janosch. "Detergent-induced cell aggregation in Pseudomonas aeruginosa". [S.l. : s.n.], 2007. http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-26614.
Pełny tekst źródłaÖstman, Johan. "Mechanisms involved in amyloid induced cytotoxicity". Doctoral thesis, Umeå universitet, Molekylärbiologi (Teknat- och Medfak), 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-541.
Pełny tekst źródłaMenzen, Tim Andreas. "Temperature-induced unfolding, aggregation, and interaction of therapeutic monoclonal antibodies". Diss., Ludwig-Maximilians-Universität München, 2014. http://nbn-resolving.de/urn:nbn:de:bvb:19-175200.
Pełny tekst źródłaNyström, Roger. "Aggregation of calcium carbonate dispersions induced by electrolytes and polyelectrolytes /". Åbo : Åbo akademi University, 2004. http://catalogue.bnf.fr/ark:/12148/cb40124838x.
Pełny tekst źródłaKnight, Grady C. "The molecular chaperone α-crystallin protects proteins from UV-induced aggregation". Diss., Georgia Institute of Technology, 1999. http://hdl.handle.net/1853/30486.
Pełny tekst źródłaXu, Meisheng. "The field-induced aggregation and magneto-optical properties in magnetic fluids". Thesis, London South Bank University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.300592.
Pełny tekst źródłaDong, Yujie. "Synthesis, photophysical properties and applications of aggregation-induced emission materials based on cyanostilbene moiety". HKBU Institutional Repository, 2016. https://repository.hkbu.edu.hk/etd_oa/313.
Pełny tekst źródłaYu, Wai Hong. "Synthesis, Characterization and application studies of new aggregation-induced emission (AIE)-active materials". HKBU Institutional Repository, 2018. https://repository.hkbu.edu.hk/etd_oa/496.
Pełny tekst źródłaBrummitt, Rebecca K. "Urea-induced dissociation of non-native aggregates of alpha-Chymotrypsinogen A kinetics, thermodynamics, and competing pathways /". Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 103 p, 2008. http://proquest.umi.com/pqdweb?did=1597632641&sid=12&Fmt=2&clientId=8331&RQT=309&VName=PQD.
Pełny tekst źródłaPhotchanachai, Songsin. "Studies on Molecular Aggregation and Degradation of Food Proteins Induced by High-Temperature Treatments". Kyoto University, 2003. http://hdl.handle.net/2433/148327.
Pełny tekst źródła0048
新制・課程博士
博士(農学)
甲第10507号
農博第1380号
新制||農||882(附属図書館)
学位論文||H15||N3862(農学部図書室)
UT51-2003-U477
京都大学大学院農学研究科応用生命科学専攻
(主査)教授 北畠 直文, 教授 小川 正, 教授 吉川 正明
学位規則第4条第1項該当
Cassidy, James. "Parsing The Aggregation- and Photodegradation-Induced Effects of Rhodamine-Sensitized Tio2 and Zro2 Films". W&M ScholarWorks, 2017. https://scholarworks.wm.edu/etd/1499450063.
Pełny tekst źródłaSuenaga, Kazumasa. "Precise Control of Highly-Efficient Solid-Emissive Property of Boron Ketoiminate". Kyoto University, 2019. http://hdl.handle.net/2433/242531.
Pełny tekst źródłaKyoto University (京都大学)
0048
新制・課程博士
博士(工学)
甲第21793号
工博第4610号
新制||工||1718(附属図書館)
京都大学大学院工学研究科高分子化学専攻
(主査)教授 田中 一生, 教授 秋吉 一成, 教授 大内 誠
学位規則第4条第1項該当
Xu, Alan. "Atomic scale characterisation of radiation damage and radiation induced precipitation in tungsten-rhenium alloys". Thesis, University of Oxford, 2015. https://ora.ox.ac.uk/objects/uuid:1ae33661-c81f-4e77-a80c-bc12ed9123d9.
Pełny tekst źródłaOhtani, Shunsuke. "Creation of Emissive and Functional Materials Based on Fused-Boron Complexes". Kyoto University, 2021. http://hdl.handle.net/2433/261618.
Pełny tekst źródłaAndersson, Karin, M. Pokrzywa, Ingrid Dacklin i Erik Lundgren. "Inhibition of TTR aggregation-induced cell death : a new role for serum amyloid P component". Umeå universitet, Institutionen för molekylärbiologi (Medicinska fakulteten), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-65622.
Pełny tekst źródłaEpub 2013 Feb 4.
Tabuchi, Arata. "Direct demonstration of involvement of protein kinase Cα in the Ca[2+]-induced platelet aggregation". Kyoto University, 2003. http://hdl.handle.net/2433/148479.
Pełny tekst źródłaLing, Karen Kar Yun. "The role of P2Y1̳ nucleotide receptor in agrin-induced AChR aggregation at the neuromuscular junctions /". View Abstract or Full-Text, 2002. http://library.ust.hk/cgi/db/thesis.pl?BIOL%202002%20LING.
Pełny tekst źródłaOn t.p. "1̳" is subscript. Includes bibliographical references (leaves 114-141). Also available in electronic version. Access restricted to campus users.
Merg, Martina [Verfasser]. "Aggregation during biopharmaceutical production of antibodies induced by UV radiation and interface exposure / Martina Merg". Ulm : Universität Ulm, 2017. http://d-nb.info/1131710495/34.
Pełny tekst źródłaArribat, Mathieu. "Acides aminés phosphole ou silole : vers de nouvelles sondes fluorescentes pour un marquage de peptide innovant". Thesis, Montpellier, 2018. http://www.theses.fr/2018MONTS144.
Pełny tekst źródłaThe first part of this work is focused on phospholyl amino acids synthesis by formation of a P-C bond. The fluorescent properties (absorption, emission and quantum yield) are modulated either by the substituent on the phosphorus atom (BH3, O, S, …) or by the aromatic skeleton of the phosphole. Peptide coupling in solution or on solid support were performed and showed the possibility to introduce such amino acids into peptide of interest. The second part of this work is dedicated to the synthesis of new functionalized phospholes for a chemoselective grafting on amino acid and peptides pendant groups (SH, NH2, OH) via PS, P-N or P-O bonds. The third part consists into the synthesis of a new class of tetraphenylsilole amino acids which exhibit AIE (aggregation-induced emission) fluorescent properties. Those compounds were successfully incorporated into di- an tri- peptides in solution and on solid support
Ito, Shunichiro. "Synthesis and Photophysical Properties of Functional Luminescent Materials Based on β-Diiminate Complexes Composed of Main-Group Metals". Doctoral thesis, Kyoto University, 2020. http://hdl.handle.net/2433/245840.
Pełny tekst źródła0048
新制・課程博士
博士(工学)
甲第22155号
工博第4659号
新制||工||1727(附属図書館)
京都大学大学院工学研究科高分子化学専攻
(主査)教授 田中 一生, 教授 秋吉 一成, 教授 古賀 毅
学位規則第4条第1項該当
Doctor of Philosophy (Engineering)
Kyoto University
DGAM
Dong, Lei. "Conception et synthèse de glyco-sondes fluorescentes pour des applications en détection". Thesis, Lyon, 2019. http://www.theses.fr/2019LYSE1153/document.
Pełny tekst źródłaWith scientific and social progress, various methods for the specific and sensitive detection of metals, proteins and other biomolecules are widely utilized in environmental protection, disease surveillance, drug therapy, agricultural production, industry and other significant areas. Fluorescent probes are widely developed based on ICT, PET, FRET and other fluorescence mechanisms, and applied to the detection of contaminants or in cell imaging. But the ACQ effect usually quenched the fluorescence intensity and thus limited the applications of organic probes in cell imaging and living systems. Therefore, the concept of aggregated-induced emission (AIE) appears as a possible solution to these problems and several fluorescent glycoclusters, glyco-probes and glyco-complexes were designed and reported for biological analysis. Our first project aimed to design and synthesize fluorescent glyco-polymers with multiple glycosides for cell targeting and drug delivery while fluorescence will allow the detection of the targeted cells. To overcome the ACQ effect and interference from natural biological background fluorescence, we conjugated dicyanomethylene-4H-pyran (DCM) and tetraphenylethene (TPE) to obtain near-infrared AIE fluorescent probes. The glycosides provided good water solubility and self-assembly in water led to detection systems and imaging cancer cells. TPE-based glycopolymers were synthesized from TPE monomers incorporating two monosaccharides by CuAAC conjugation and these monomers were polymerized by either CuAAC or thiol-ene “click” reactions. The TPE-based glycopolymers did not display a large chain length (typically less than 7 units) and the expected fluorescent properties could not be reached. We then designed and synthesized glyco-dots self-assembled by DCM probes and TPE-based glycoclusters. The glyco-dots displayed high water-solubility and selective response to peroxynitrite (ONOO-) both in vitro and in cell assays. The glyco-dots could detect endogenous and exogenous ONOO- but no specific cell recognition. We designed and synthesized AIE fluorescent probes which could self-assemble with TPE-based glycoclusters. The resulting glyco-dots were readily water soluble and displayed excellent sensitivity and selectivity for thiophenol detection in vitro and in environmental water samples. We finally combined both TPE and DCM moieties to synthesize a novel AIE fluorophore (TPE-DCM) with long-wavelength emission. Then conjugation with glycosides through CuAAC led to AIE fluorescent probes with long-wavelength emission, excellent water-solubility. Application to the detection of glycosidases in vitro and in cell assays or animal models was possible with these probes
Nylander, Sven. "Thrombin/ADP-induced platelet activation and drug intervention /". Linköping : Univ, 2005. http://www.bibl.liu.se/liupubl/disp/disp2005/med885s.pdf.
Pełny tekst źródłaMenzen, Tim Andreas [Verfasser], i Wolfgang [Akademischer Betreuer] Frieß. "Temperature-induced unfolding, aggregation, and interaction of therapeutic monoclonal antibodies / Tim Andreas Menzen. Betreuer: Wolfgang Frieß". München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2014. http://d-nb.info/1060632438/34.
Pełny tekst źródłaLau, Wai Sum. "Synthesis, characterization and application studies of cyanostilbene-based molecular materials with aggregation-induced emission (AIE) characteristics". HKBU Institutional Repository, 2014. https://repository.hkbu.edu.hk/etd_oa/70.
Pełny tekst źródłaWang, Jin-Hui. "Rhenium complexes based on triazolyl derivatives : from synthesis, structural and theoretical characterization to application as radiopharmaceuticals or fluorophores". Thesis, Toulouse 3, 2017. http://www.theses.fr/2017TOU30329/document.
Pełny tekst źródłaRhenium complexes play a significant role in nuclear medicine. Rhenium has been widely used as a surrogate of technetium for a long time, and the promising physical features of 186Re and 188Re, make 186/188Re-complexes promising candidates as therapeutic radiopharmaceuticals.Similarly, the interesting photoactive and photoluminescence properties of non-radioactive Re-complexes make them excellent catalysts, luminescent materials and imaging sensors.Thus, in this work, our goal was to (i) develop, using a click chemistry strategy, multidentate ligands for the stabilization of different rhenium cores [Re(CO)3]+ and [ReO]3+ (M = Re or 188Re) as well as the analogous 99mTc-cores in some examples, (ii) assess the potential of the rhenium(technetium) complexes as imaging (natRe or 99mTc) or therapeutic (188Re) agents. To do so, two rhenium(technetium) specific-chelating systems were used: a semi-rigid tripodal system in the second chapter and a pyta moiety in the third chapter, these two chelators being developed previously in our group. Thus, based on a N2O tridentate click ligand, two different studies were carried out in chapter II. In the first one, two synthetic pathways to a range of potentially N3O tetradentate ligands, designed to coordinate rhenium cores as well as their coordination behaviors towards different rhenium cores (oxidation states +I and +V), were investigated. The first radiolabeling results combined with the recent work reported by Dugave and co-workers indicated that this ligand could be a promising 99mTc-chelator for nuclear imaging applications. As perspectives to this work, the extension of the radiolabelling work using the [188ReVO]3+ core should be performed, and the in vitro stability should be tested under physiological conditions in human plasma and by cysteine exchange experiments. The second study was focused on the development of novel hypoxia-selective 99mTc radiopharmaceuticals. Our semi-rigid tripodal click framework was decorated with an appended nitro group (either a nitrobenzyl group or a metronidazole (Mtz) unit). Different positions were considered and at least only two metronidazole (Mtz)-containing ligands and one nitro group-containing ligand as well as their corresponding tricarbonyl rhenium(I) complexes were obtained and characterized, in particular by electrochemistry. The reduction potentials of NO2 group in complexes [Re(CO)3Cl(L2)] and [Re(CO)3(L6)] were similar to those of reported hypoxic imaging agents, prompting us to further investigate other properties of these complexes. Chapter III was focused on the study of AIE (aggregation-induced emission) effect in tricarbonyl Re(I) complexes, the association of this effect with the intrinsic properties of Re(I) complexes being expected to lead to very attractive compounds. To do that, we combined an organic fluorophore (PBO) which exhibits excellent stability and optical properties, with a tricarbonylrhenium(I) complex based on a pyta unit (either a 2-pyridyl-1,2,3-triazole or a 2-pyridyl-1,2,4-triazole ligands). Four compounds were studied. The X-Ray structures revealed spectacular discrepancies between the two first triazole-based complexes ReL8 and ReL9. Moreover, this study being a novel orientation in our group, this work is a great starting point for further investigations. Various organic dyes and/or structural modifications of the organic moiety will soon be considered in order to develop highly emissive rhenium(I) luminescent probes
Tominaga, Masato. "Functional π-Conjugated Materials Based on Structure of o-Carborane". 京都大学 (Kyoto University), 2014. http://hdl.handle.net/2433/188607.
Pełny tekst źródłaWeir, Steven. "Effect of fermentation media on the polymer-induced aggregation of microorganisms and the effect on subsequent centrifugation". Thesis, University of Huddersfield, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.261466.
Pełny tekst źródłaUpamali, Karasinghe A. Nadeeka. "Carbazole-Based, Self-Assembled, Π-Conjugated Systems As Fluorescent Micro And Nanomaterials - Synthesis, Photophysical Properties, Emission Enhancement And Chemical Sensing". Bowling Green State University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1323099511.
Pełny tekst źródłaDong, Wenyue [Verfasser]. "The Design and Synthesis of Conjugated Polymers with Aggregation-Induced Emission and Their Application in Fluorescence Sensing / Wenyue Dong". Wuppertal : Universitätsbibliothek Wuppertal, 2015. http://d-nb.info/1076929885/34.
Pełny tekst źródłaDewsbury, Bryan M. "Artificially induced aggregation of fauna and their effects on nutrient regimes and primary producers in an oligotrophic subtropical estuary". FIU Digital Commons, 2006. http://digitalcommons.fiu.edu/etd/2786.
Pełny tekst źródłaYoshii, Ryosuke. "Synthesis of Highly-Functional Polymers Using Characteristics of Four-Coordinated Boron-Complexes with Boron-Nitrogen Bonds". 京都大学 (Kyoto University), 2014. http://hdl.handle.net/2433/188610.
Pełny tekst źródłaMcKeon, Kristin Dianne. "Albumin Adsorption: Inferences of Protein Interactions Measured by Sedimentation both Between Species and Induced by Denaturing". Thesis, Virginia Tech, 2008. http://hdl.handle.net/10919/32082.
Pełny tekst źródłaA dynamic laser light scattering instrument will be utilized to measure the differences in size and determine the amount of aggregation. Our lab has developed a z-axis translating laser light scattering device (ZATLLS) that has been used to measure the sedimentation velocity of several different materials in solution. In this case, bovine serum albumin (BSA) will be adsorbed onto polystyrene particles and the particle settling velocity determined. The settling solution viscosity and density will also be ascertained, so Stokeâ s law can infer the average aggregate size of each experiment. BSA-coated polystyrene particles displayed a more controlled settling behavior compared to non-coated polystyrene particles. Although the BSA-coated particles had a smaller sedimentation velocity, a larger aggregate size was found due to the greater solution viscosity. Therefore, the ZATLLS instrument can be employed to measure sedimentation velocities of multiple interactions and the aggregation level inferred.
Although most albumin molecules are remarkably similar, there are subtle differences in amino acid residues, length, and charge. Sedimentation velocities for human serum albumin (HSA) coated polystyrene particles and BSA-coated polystyrene particles only had a small difference. However an almost 50% higher solution viscosity was measured in BSA experiment solutions, and resulted in the slower settling of the larger aggregates compared to HSA-coated particles. Viscosity calibration curves for each albumin species were used to determine the amount of protein desorbed from the particles during the settling process. The larger solution viscosity for BSA-coated particle experiments led to a much larger degree of desorption. HSA was shown to be the more stable albumin species when adsorbed onto polystyrene particles.
Temperature denaturing was performed to aid in the determination of the stability of BSA. Reversible and irreversible conformational changes in BSA were produced at 46ºC and 76ºC respectively. The solutions were cooled to room temperature before adsorption onto polystyrene particles and the sedimentation velocities measured. A 50% difference in average viscosity between the reversibly and irreversibly changed BSA was found. This caused the larger aggregates formed in the 76ºC BSA experiments to have an almost equivalent sedimentation velocity to those in the reversibly denatured BSA experiments. Average aggregate size for reversibly denatured BSA was well within the ranges found for non-denatured BSA. In conclusion, irreversibly denatured BSA formed larger aggregates and was more likely to desorb from the polystyrene particles than reversibly changed BSA.
Master of Science
Abeyawardhane, Dinendra L. "Biometal-Induced Structural Consequences of α-Synuclein – the Parkinson’s Disease Protein". VCU Scholars Compass, 2019. https://scholarscompass.vcu.edu/etd/5909.
Pełny tekst źródłaShorikova, D. V. "The collagen-induced platelet aggregation and artery status in patients with arterial hypertension and heart failure with preserved ejection fraction". Thesis, БДМУ, 2021. http://dspace.bsmu.edu.ua:8080/xmlui/handle/123456789/18601.
Pełny tekst źródłaViglianti, L. "THE HETEROARYLETHENES: SYNTHESIS, ELECTROCHEMISTRY AND INVESTIGATION ON THE AGGREGATION-INDUCED EMISSION OF A PROMISING CLASS OF MOLECULES WITH LUMINESCENT PROPERTIES". Doctoral thesis, Università degli Studi di Milano, 2015. http://hdl.handle.net/2434/338784.
Pełny tekst źródłaDeng, Jiun-Ping, i 鄧君屏. "Shear induced aggregation in liquid suspensions". Thesis, 2005. http://ndltd.ncl.edu.tw/handle/77288998096356858609.
Pełny tekst źródła長庚大學
化工與材料工程研究所
93
The aggregation of an initially well-dispersed suspension of particulates due to shear-induced collision is examined in laminar flow and in turbulent flow. Experiments in laminar flow are conducted by a cone-and-plate device on latex particles suspended in aqueous glycerol while experiments in turbulent flow are conducted by stirred tank. The corresponding shear rate in laminar flow and in turbulent flow is calculated as a function of the stirring rate. The rate of aggregation growth is measured as a function of time for different constant shear rate and suspension compositions. The experimental results show that the aggregate size increases rapidly in the early stage and then gradually reaches an equilibrium value due to the influence of hydrodynamic shear forces. An aggregation model is developed in this work to recover the collision efficiency and the equilibrium aggregate size from the experiment data.
Lee, Tai-chun, i 李岱軍. "Conformation Induced Molecular Aggregation in Poly(3-hexylthiophene)". Thesis, 2010. http://ndltd.ncl.edu.tw/handle/54207782765945143457.
Pełny tekst źródła輔仁大學
化學系
98
In this research, we’d like to study the morphology of P3HT in solution by controlling spin rate in solution state and also its morphology in films. UV-Vis and PL were used to monitor the state of aggregation under different shear force. Viscosity measurements were conducted to elucidate molecular conformation of P3HT in solution and to explain the results from UV-Vis and X-ray measurements. We found morphology of films prepared by aggregation of P3HT itself in solution was different from that prepared by extra shear force. Aggregation of P3HT itself in solution was destroyed during the film formation by spin-coating process. Packing of P3HT by extra shear force was maintained during the spin-coating process because shear force pushed the P3HT molecules in helical form to pack together tightly furing the stirring process. The results were different by using different solvents and they are related to the conformation of P3HT in solution. With this concept of organic solar cells can be used to process the above. We can create without annealing can produce highly ordered P3HT in close gatherings of activity layer, in order to save resources in the production of batteries and costs.
Lin, Li-yang, i 林利陽. "Polypeptides containing luminescent units with aggregation-induced emission property". Thesis, 2015. http://ndltd.ncl.edu.tw/handle/j25965.
Pełny tekst źródła國立中山大學
材料與光電科學學系研究所
103
First chapter: Tetraphenylthiophene (TP) with aggregation-enhanced emission (AEE) property was used as terminal fluorophore of the water-soluble poly(γ-propargyl-L-glutamate) (PPLG)-based polymers of TP-PPLG-g-MEO2. In this study, we research various TP-PPLG-g-MEO2 affect aggregation of luminescence. In water, when concentration of TP-PPLG-g-MEO2 achieve CMC, TP-PPLG-g-MEO2 will aggregation to nanoparticle and suddenly increasing emission, and when heating over LCST, TP-PPLG-g-MEO2 will contract and also enhance emission. We use TP-PPLG-g-MEO2 “salt out effect” property to probe salt in water, salt have interaction with side chain MEO2 and peptide main chain to make TP-PPLG-g-MEO2 contract together and change secondary structure to b-sheet conformation to enhance emission. In the strong alkaline media, TP-PPLG-g-MEO2 backbone change to random coil conformation that break intramolecular hydrogen bonding and loss LCST property, TP-PPLG-g-MEO2 become is a micelle like structure, core was hydrophobic TP and shell was hydrophilic polypeptide and particle size about 500nm measure by DLS. In random coil conformation, chain end hydrophobic TP have strong aggregation than in the a-helical and have strong aggregation emission peak. At last, we use TP-PPLG-g-MEO2 to detect BSA. When TP-PPLG-g-MEO2 mixture with BSA, TP-PPLG-g-MEO2 will fall in to BSA and separate to decrease TP-PPLG-g-MEO2 aggregation and emission. Second chapter: We synthesis polypeptide contain TPA pendent by click reaction. The resulting PPLG-g-TPA contains the crystalline TPA side groups, Tm about 145 oC and is therefore high Tg materials with the desired AIE activity. Due to side chain TPA, PPLG-g-TPA have piezofluorochromic property that have two color between crystal and amorphous state. The lone pair electrons of nitrogen atom in TPA side groups inherit PPLG-g-TPA the sensitivity toward acid HCl and metal ions. Emission of PPLG-g-TPA was progressively decreased upon increasing the amounts of HCl and metal ions in the solutions that can be acid or metal ions sensor.
Chang, Jui-Wen, i 張瑞文. "Aggregation-Induced Emission Characteristics of Tetraphenylethene-Capped Dipeptide Materials". Thesis, 2014. http://ndltd.ncl.edu.tw/handle/43nzds.
Pełny tekst źródła國立交通大學
材料科學與工程學系所
103
Supramolecular self-assembly hydrogel materials is ubiquitous in materials science and biology. Self-assembly provides routes to a range of materials with regular structures. And others advantages to developed in this process. In this thesis, we developed the novel dipeptide supramolecular hydrogel with self-assembly gelation enhanced fluorescence emission. Tetraphenylethene (TPE) is almost non-emissive when molecularly dissolved in solution but turns to be highly fluorescent in aggregate states. The hydrogelator was constructed by using the TPE as the hydrophobic part and four different dipeptide (Gly-Gly、 Ser-Ser、 Asp-Asp and Tyr-Asp) as the hydrophilic component. We investigated these amphiphilic low-moleculars weight of the gelation pH value, performance of rheology, morphology and optical properties of the AIE characteristics. Each of the luminophore hydrogels has received different interesting results: TPE-GG is the minimum molecular weight of TPE-capped peptide forms the supramolecular hydrogel. TPE-SS is the minimum molecular weight of TPE-capped peptide forms the supramolecular hydrogel under neutral condition. TPE-DD could form the hydrogel under the acid condition(pH value= 4.3) and could be a pH sensor. TPE-YD can form the strongest hydrgel under the acid condition(pH value= 4.3) and also can form stable hydrgel under neutral condition. TPE-SS and TPE-YD can form hydrogel under neutral condition. These materials are potentially useful for develop 3D cell culture in the future. TPE-DD could change the emission properties under different pH values, which may be promising a biomaterial as an active element of biosensors.
Lee, Ying-Nung, i 李怡儂. "Aggregation of SARS-CoV membrane protein induced by heat". Thesis, 2003. http://ndltd.ncl.edu.tw/handle/31279254307817598750.
Pełny tekst źródła慈濟大學
分子生物及細胞生物研究所
92
Severe acute respiratory syndrome (SARS) is a new respiratory illness caused by the infection of a novel coronavirus called SARS-CoV. Previous studies in our laboratory found that SARS-CoV membrane protein could be easily detected using Western blotting in non-denaturing condition but not in regular denaturing condition. Using Western blotting analysis and in vitro transcription /translation system (isotope-labeled), the aggregated membrane protein with the thermal denaturation (different temperature or different boiling time) was detected in the stacking gel. Therefore, the thermal aggregation of SARS-CoV membrane protein results in the inefficiency in detecting the membrane proteins. The aggregated membrane protein could not be re-dissolved by different denaturants: 2%SDS, 6Murea, 1%Triton-X100 Furthermore, the hydrophobic regions with amino acid residues from 61 to 90, from 91 to 100, and from 136 to 170 are essential for the aggregation of membrane protein. The membrane mutant protein with deletion from a.a.91 to 100 could be aggregated with wild type membrane protein when induced by heat. These results indicate that aggregation of SARS-CoV membrane protein induced by heat is through extensive interaction in hydrophobic domains. Therefore, if SARS-Cov membrane protein needs to be analyzed using SDS-PAGE, regular boiling treatment should be avoiding. Thermal aggregation of SARS-CoV membrane protein may be also responsible for the inactivation of this virus by heat. Thermal aggregation of SARS-CoV membrane protein also provides a model to study the protein aggregation.
Lin, Ya-Lin, i 林雅琳. "Platelet Aggregation Induced by Serotype Polysaccharide from Streptococcus mutans". Thesis, 2002. http://ndltd.ncl.edu.tw/handle/15726071971429304067.
Pełny tekst źródła國立臺灣大學
微生物學研究所
90
Abstract Infective endocarditis is characterized by the formation of vegetation, which is composed mainly of fibrin-platelet matrix, on damaged heart valves. Many bacterial proteins along with bacteria are able to activate platelets, and therefore play a crucial role in the pathogenesis because local activation of platelets contributes to the formation of vegetation. Using platelet aggregometry, we demonstrated in this study that Streptococcus mutans could aggregate platelets in rabbit platelet-rich plasma preparation in an irreversible and saturation manner. Both the heat sensitive and resistant bacterial components were responsible for the aggregation. However, their ability to aggregate platelets as well as the effects of heat inactivation were variable among different strains. Heat-stable serotype specific polysaccharide, which is composed of glucose-rhamnose polymers, is one of the major components responsible for platelet aggregation. The mutant strain Xc24R, defective in the synthesis of rhamnose backbone, was less effective in inducing aggregation compared to the wild type strain Xc and the other mutant Xc31, which is defective in the formation of glucose sidechain. In addition, polysaccharide extract from Xc and other laboratory strains, but not from Xc24R, weak in induced platelet aggregation. Whole bacteria or partially purified polysaccharide could not induce platelet aggregation in the absence of plasma, although polysaccharide could adhere directly to rabbit platelet and induce shape change. Absorption of plasma components by Xc strain could induce platelet aggregation, whereas absorption with Xc24R mutant strain failed to induce aggregation. Analysis by flow cytometry showed that S. mutans polysaccharide bind not only to rabbit platelets but also to human platelets. These results suggest that S. mutans could bind directly to platelets through its serotype specific polysaccharide. In the presence of plasma, other bacterial products might aggregate platelets directly or indirectly through binding of plasma components.
Huang, Pai-Ching, i 黃百慶. "Dexmedetomidine Enhances Adenosine Diphosphate-Induced Platelet Aggregation and Activation". Thesis, 2012. http://ndltd.ncl.edu.tw/handle/77082214881544956058.
Pełny tekst źródła中國醫藥大學
臨床醫學研究所碩士班
100
As an integral part in hemostasis, platelet has also been proved to play an important role in inflammation. Platelet activation was the very first step to participate in either hemostasis or inflammation. Many factors or molecules have been demonstrated as platelet stimulants. More and more platelet receptors were then discovered as well. The complicated networks of these receptor functions were composed of several distinct pathways. We could measure the different chemical products or physical phenomenons and thereby study the possible pathways in which were involved. In Taiwan, Precedex (dexmedetomidine) was used, as a sedative agent with analgesic effect, more and more frequently in ICU and operating room. In this study, we were the first to described that dexmedetomidine might have pro-aggregatory effect on platelet in vitro. We used several indicators of platelet activation, such as platelet aggregation, P-selectin, platelet-leukocyte aggregate, [Ca2+]i, and ATP, to measure the effect of dexmedetomidine on ADP-induced platelet aggregation and activation. Moreover, we used alpha antagonists to prove the above effect was through the activation of alpha-2 receptor on platelets. Then we compared the platelet counts before and after use of dexmedetomidine on ICU patients to preliminarily find out it’s possible effects in vivo. In this series of experiments, we have proved that dexmedetomidine could enhance ADP-induced platelet activation and aggregation through alpha-2 receptor in vitro. We should be cautious about prescribing dexmedetomidine for the patients with high risk of thromboembolism or inflammatory diseases.
Hsieh, Meng-Chieh, i 謝孟潔. "Aggregation Induced Photodynamic Therapy Enhancement of Binary Photosensitizers system". Thesis, 2012. http://ndltd.ncl.edu.tw/handle/08756709360896622089.
Pełny tekst źródła國立中興大學
生醫工程研究所
100
For the purpose to enhance the efficiency of PDT (photodynamic therapy) and extend its application; the binary system complexes were designed and synthesized with conjugating a chromophore (3, 6-bis-(1-methyl-4-vinyl- pyridinium) carbazole diiodide, BMVC) to mono-, bis- and trishydroxyl photosensitizers, respectively. BMVC plays the role of cancer cells recognizer; AIEE (aggregation-induced emission enhancement) generator and FRET (Fluorescence Resonance Energy Transfer) donor. The self assembling properties of these binary conjugates result in different degrees of AIEE and then achieve the formations of FONs (fluorescent organic nanoparticles), which present efficient FRET and singlet oxygen generations. Biologically, FONs-photosensitizers from these compounds were much more phototoxicities to cancer cell than to normal cell without significant dark toxicity. This improved photodynamic activity might be due to the aggregation of compounds in the cell that form FONs which can promote PDT and are observed in cancer cell but not normal cell. In the second stage, we screened the compounds which offered better PDT efficiency from the SAR (structure activity relationship) and modified the molecular structure furtherly. We embedded the metal ion such as Zn2+ and Pt2+ in the center of porphyrin to affect the activation area of FRET and prolong the triplet state life time. Then measured the singlet oxygen generation rate ability by using 1, 3-diphenylisobenzofuran (DPBF), to make sure the metallo binary system has better PDT efficiency than metal-free binary conjugates.
Chen, Jyun-Wei, i 陳俊瑋. "Novel Aggregation-Induced Emission Fluorophores Applied on Cellular Studies". Thesis, 2017. http://ndltd.ncl.edu.tw/handle/88facz.
Pełny tekst źródła國立中興大學
化學工程學系所
106
In this study, we focused on developing the newly organic molecules on the therapy and diagnosis of cancers. In the experiments, we synthesized some simple fluorescent organic molecules and enhanced their performances in the cells by the unique properties of Aggregation-Induced Emission (AIE). First, we synthesized an asymmetric 1, 6-disubstituted naphthalene (NV-12P), which can generate particular reactive oxygen species to undergo type I photodynamic therapy under irradiation. Furthermore, this compound can specifically localize in mitochondria in cancer cells to exhibit selective dark cytotoxicity, and exhibit efficient photodamage in cancer cells due to intracellular bright spots. Results suggested that this compound can be a potential dual-toxic efficacy molecule. Additionally, a water-soluble pH sensor ADA was designed and synthesized based on the molecular design of photo-induced electron transfer (PET) and intramolecular charge transfer (ICT). The fluorescent emission response against a pH value located in the range of 3~6, which is suitable to label intracellular pH dependent microenvironments. Finally, we not only used ADA to identify intracellular pH gradients between different cell lines, but also developed ADA as a fluorescent molecule that labeled normal cells by the unique fluorescence intensity of ADA in normal cells, making it effective as the control group at the time of cancer diagnosis.
Mandal, Kuheli. "Fluorescent Bioimaging Probe from Aggregation Induced Emission Active Molecule". Thesis, 2019. http://hdl.handle.net/10821/8341.
Pełny tekst źródłaLIN, YUN-RU, i 林韻茹. "Investigation of Aggregation-Induced-Emission Behavior for Tetraphenylethylene-Based Polymer". Thesis, 2017. http://ndltd.ncl.edu.tw/handle/j97wv6.
Pełny tekst źródła逢甲大學
化學工程學系
105
In this study, we successfully synthesized a small molecules with tetraphenylethylene as the basic structure and the correspounding polymer. Two methods were tested when small molecules was synthesized. The product synthesized by McMurry reaction found to be a good method for the synthesis of small molecules. The molecules were polymerized to obtain the corresponding macromolecule materials. Then, the absorption spectra, fluorescence spectra, aggregation-induced emission effect and quantum yield were measured, respectively. The results showed that the absorption and fluorescence peaks of polymer were bathochromic shifted as conjugation chain was increased. The size of the polymer gradually increased with the increasing of the water fraction of solvent and begins to decrease after the water fraction reach 70% water percentage. The result agrees with the fact that the smaller size particle favor closer packing of polymer molecules and showed brighter fluorescence. In the polymer systems, the intramolecular rotations of the phenyl rotors are already partly restricted at molecular level due to the steric effect of polymer chains. Finally, the thermal stability was measured, the Td was 380℃, and the polymer showed nice thermal stability.
Chang, Jean-Yun, i 張景雲. "TGF-beta induced aggregation of trafficking protein TRAPPC6A and apoptosis". Thesis, 2009. http://ndltd.ncl.edu.tw/handle/77836481218037923263.
Pełny tekst źródła國立成功大學
分子醫學研究所
97
Protein aggregation is central to the development of neurodegeneration such as Alzheimer’s disease. Here, we isolated a TGF-beta1-induced trafficking protein particle complex 6A, designated TPC6Adelta, which binds tumor suppressor WOX1 (also named WWOX or FOR). Compared to the wild type (22 kDa), TPC6Adelta has an N-terminal truncation of amino acid 29-42. TPC6A is a component of transport protein particle (TRAPP) complex in vesicle trafficking from ER to the Golgi complex in yeast. In contrast, mammalian TPC6A mainly localizes in the nuclei and at the perinuclear area. By co-immunoprecipitation, endogenous WOX1 was shown to interact with TPC6A. By FRET analysis, TPC6Adelta physically bound to the C-terminal tail (D3 domain) and ADH/SDR domain of WOX1. Prolonged stimulation of cells with TGF-beta1 reduced the binding of TPC6Adelta and WOX1, which ultimately resulted in TPC6Adelta aggregation. Notably, plaque-like TPC6A aggregates were found in the hippocami of patients with Alzheimer’s disease (AD) and transgenic AD mice model. Most importantly, TPC6A aggregates (> 0.2 micrometer in diameter) were more abundant in the younger nondemented human populations (60±13.3 years old, n=11) than in the older AD patients (81±9.7 years old, n=27), suggesting TPC6A is a likely marker for AD development. Time-lapse microscopy revealed that TGF-beta induced aggregation of intracellular TPC6Adelta. The aggregation may lead to Abeta superproduction and neuronal death. WOX1 and TPC6Adelta nullified each other in causing apoptosis, implying that WOX1 prevents aggregation of TPC6Adelta in cells. Together, here we discovered a novel mechanism for the pathogenesis of AD, which involves TGF-beta-induced TPC6A aggregation. Mechanistically, TGF-beta induces dissociation TPC6A from WOX1, followed by TPC6A aggregation, Abeta deposition and plaque formation.
Przybycien, Todd Michael. "Structure, Function and Aggregation Kinetics in Salt-Induced Protein Precipitation". Thesis, 1989. https://thesis.library.caltech.edu/686/3/przybycien-tm_1989.pdf.
Pełny tekst źródłaSalt-induced precipitation is a biological separation technique that exposes proteins to unnatural environments. Macromolecular-scale issues of activity, structure, and aggregation have been addressed as a function of governing parameters.
The effects of salt type and concentration on protein solubility and recoverable activity were studied using α-chymotrypsin (αCT) as a model protein and five salts spanning the lyotropic series. Unaccounted for salt-protein interactions and changes in protein physical properties were the likely source of discrepencies between the experimental and theoretical solubility behavior. Active protein recovery was a function of salt type, but not concentration. A salting-out performance parameter was identified; an optimum salt may exist for a particular protein.
αCT precipitates from the solubility-activity study were examined for perturbations in secondary structure via Raman spectroscopy and in active site tertiary structure via electron paramagnetic resonance spectroscopy. NaBr, KBr, and KSCN-induced precipitates had increased β-sheet and decreased α-helix contents; these changes were correlated with active protein yields. Spectra of spin-labelled precipitates indicated that the active site remains intact. Molecular modelling was used to estimate changes in the dipole moment and hydrophobic surface area for the altered precipitates. A general mechanism for the precipitation of globular proteins was proposed.
The generality of secondary structure changes was explored for twelve different proteins via Raman spectroscopy. KSCN-induced precipitates exhibited increased β-sheet and decreased α-helix contents; structural changes for Na₂SO₄-induced precipitates were less significant. The β-sheet increase may occur at the expense of α-helix segments. β-sheet increases were correlated with the fraction of charged residues and the surface area of the native protein. α-helix decreases were correlated with the dipole moment and helical content of the native protein.
The effects of temperature, protein concentration, salt type, and salt concentration on αCT aggregation kinetics were studied. Stopped-flow turbidimetry indicated that temperature and salt concentration effects are exerted through changes in protein solubility. Protein concentration effects are well-described by Smoluchowski's collision equation. The aggregation of partially inhibited αCT demonstrated poisoning behavior. Solute particle radius distributions determined by dynamic laser light scattering indicated that aggregation denends on the supersaturation. A detailed population balance model, accounting for specific and nonspecific quaternary interactions, was developed.
(9748970), Hengming Qiu. "DNA Signal Induced Fusion And Aggregation Behaviors of Synthetic Cells". Thesis, 2020.
Znajdź pełny tekst źródłaCHEN, PIN-RUEI, i 陳品叡. "Aggregation-induced emission in tetrasubstituted tetraphenylethene derivatives: synthesis and characterization". Thesis, 2019. http://ndltd.ncl.edu.tw/handle/kj8s8y.
Pełny tekst źródła逢甲大學
化學工程學系
107
Luminescent materials with aggregation-induced emission (AIE) properties have attracted many people's interest in recent years due to their application in, optoelectronic components, fluorescent probes, chemical sensor, and biosensor. Tetraphenylethene (TPE) is a compound that often used to investigate luminescence properties with significant AIE. In this study, TPE derivatives 9, 14, 15, 16, 18a-d with tetra-substituted structure were successfully synthesized, and their absorption spectra, fluorescence spectra, aggregation-induced luminescence, liquid fluorescence quantum yield and electrochemical properties were studied. Based on the red-shift of absorption and fluorescence spectra for compounds 14, and 15, the conjugation growth was observed. In the aggregation-induced emission test, it was found that the fluorescence intensity increased with decrease of the solution solubility, and red-shift of fluorescence occurred. The compound 14, with Tetramethyl silane (TMS) substituents, showed the highest fluorescence quantum yield (56.7%). Moreover, different alkyl chains led to different fluorescence quantum yield. The oxidation potential of the compounds was determined by cyclic voltammetry and the energy of the highest occupied molecular orbital (HOMO) and the lowest unoccupied molecular orbital (LUMO) was estimated. The thermal decomposition temperatures of the compounds were above 300oC revealing good thermal stability.