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Gotowe bibliografie tematyczne / AGARD-B

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Gotowa bibliografia na temat „AGARD-B”

Autor: Grafiati

Data publikacji: 6 września 2023

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Artykuły w czasopismach na temat "AGARD-B"

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Damljanović, Dijana, Đorđe Vuković, Goran Ocokoljić i Boško Rašuo. "Convergence of transonic wind tunnel test results of the AGARD-B standard model". FME Transactions 48, nr 4 (2020): 761–69. http://dx.doi.org/10.5937/fme2004761d.

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AGARD-B is a widely-used configuration of a standard wind tunnel model. Beside its originally intended application for correlation of data from supersonic wind tunnel facilities, it was tested in a wide range of Mach numbers and, more recently, used for assessment of wall interference effects, validation of computational fluid dynamics codes and validation of new model production technologies. The researchers and wind tunnel test engineers would, naturally, like to know the "true" aerodynamic characteristics of this model, for comparison with their own work. Obviously, such data do not exist, but an estimate can be made of the dispersion of test results from various sources and of the probable "mean" values of the aerodynamic coefficients. To this end, comparable transonic test results for the AGARD-B model at Mach numbers 0.77, Mach 1.0 and Mach 1.17 from six wind tunnels were analyzed and average values and dispersions of the aerodynamic coefficients were computed.

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Tuling, S., B. Vallabh i M. F. Morelli. "Compressibility effects for the AGARD-B model". Aeronautical Journal 119, nr 1214 (kwiecień 2015): 543–52. http://dx.doi.org/10.1017/s0001924000010605.

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AbstractA numerical study of the flow topologies over the 60° delta wing of the AGARD-B model at Mach 0·80 has revealed that vortex bursting occurs between 13°-15° angle-of-attack, while vortex separation occurs above 18°. These aerodynamic features have been identified as additional comparison criteria which need to be replicated for facilities using the model for calibration or inter-tunnel comparison purposes. The numerical simulations were performed using ANSYS Fluent V13, a structured mesh with near wall treatment and the Spalart-Allmaras and κ-ω SST turbulence models, and validated experimentally in a 5′ × 5′ transonic facility. Other aspects not previously identified or studied are firstly a recovery shock between the primary and secondary vortex that exists only when vortex bursting occurs, and secondly the lack of a shock between the wing and vortex when the flow topology corresponds to the centreline shock region as observed in other studies.

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Sawada, Hideo, Shinichi Suda i Tetsuya Kunimasu. "Magnetic Suspension of an AGARD-B Model in 6 D.O.F." JOURNAL OF THE JAPAN SOCIETY FOR AERONAUTICAL AND SPACE SCIENCES 54, nr 629 (2006): 276–78. http://dx.doi.org/10.2322/jjsass.54.276.

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Panasenko, A. V. "Results of Supersonic Gas Flow Calculations near AGARD-B Model". Physical-Chemical Kinetics in Gas Dynamics 21, nr 2 (2020): 1–8. http://dx.doi.org/10.33257/phchgd.21.2.887.

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YASUE, Kanako, i Keisuke SAWADA. "Effect of Model Deformation on Aerodynamic Coefficients for the AGARD-B Wind Tunnel Model". TRANSACTIONS OF THE JAPAN SOCIETY FOR AERONAUTICAL AND SPACE SCIENCES 54, nr 185/186 (2011): 163–72. http://dx.doi.org/10.2322/tjsass.54.163.

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Vidanovic, Nenad, Bosko Rasuo, Dijana Damljanovic, Djordje Vukovic i Dusan Curcic. "Validation of the CFD code used for determination of aerodynamic characteristics of nonstandard AGARD-B calibration model". Thermal Science 18, nr 4 (2014): 1223–33. http://dx.doi.org/10.2298/tsci130409104v.

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The application of Computational Fluid Dynamics (CFD) is often motivated by the limitations of measurement techniques, economic limitations, and complex model geometry or, as it is in this case, the unavailability of appropriate test model geometry. CFD was used to assess and evaluate scenario that cannot be investigated experimentally and was shown to be an efficient and economical option to experimental setup. Because of that, there is a strong need for a validation procedure and assessment of the data obtained by numerical simulation. A combined experimental/numerical procedure is described for determination and estimation of subsonic and supersonic aerodynamic behavior of an AGARD-B model with a nonstandard nose configuration. Conducted numerical aerodynamic calculations needed to be satisfied via experimental tests so, the CFD code validation procedure required experimental data that characterize the distributions of measured aerodynamic forces and moments which act upon the test model. Validation of the CFD was achieved by performing the calculation for the model with the standard nose shape as well, and by comparing the results of the CFD calculations with available experimental data for the model with the standard nose configuration. Comparison demonstrated very good agreement between numerically and experimentally obtained results. It was concluded that the numerical prediction for the similar nonstandard model configuration could be accepted as reliable and used to estimate the corrections needed when interpreting the available data. The effects of the different nose shape were found to be small and noticeable mainly in the pitching moment coefficient. This work also demonstrates the application of CFD for the purpose of proving a qualitative and quantitative prediction of the aerodynamics behavior.

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Damljanović, Dijana, Đorđe Vuković, Goran Ocokoljić, Biljana Ilić i Boško Rašuo. "Wind Tunnel Testing of ONERA-M, AGARD-B and HB-2 Standard Models at Off-Design Conditions". Aerospace 8, nr 10 (22.09.2021): 275. http://dx.doi.org/10.3390/aerospace8100275.

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Published results for standard wind tunnel models at non-standard test conditions are quite rare and/or may not be available. It has been found that those results are a useful aid in preparations for a number of wind tunnel tests in the Military Technical Institute (VTI) in Belgrade. Test campaigns of standard models at non-standard conditions are performed to serve as an internal database for future wind tunnel tests in such environments. Those tests, that partially overlap the referenced Mach number and/or angle of attack ranges, are conducted in different VTI’s test facilities; different model sizes and support stings were used. The standard models used in static measurements in VTI, ranging from simple missile shapes and re-entry bodies to complicated airplanes, are briefly described and sample non-standard test results are given. The correlation of the test results among models and facilities has been done with references in the available ranges, and, after confirming a good agreement, it is assumed that the results are also valid in the extended ranges of conditions. These results may be useful for researchers in other wind tunnel facilities and for those who handle CFD tools.

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Gesner, B. Edward. "Agard, Frederick B. A Course in Romance Linguistics. Vol. 1: A Synchronic View. Washington, DC: Georgetown University Press, 1984.; Agard, Frederick B. A Course in Romance Linguistics. Vol. 2: A Diachronic View. Washington, DC: Georgetown University Press, 1984Agard, Frederick B. A Course in Romance Linguistics. Vol. 1: A Synchronic View. Washington, DC: Georgetown University Press, 1984. Pp. 242. $19.95 US.Agard, Frederick B. A Course in Romance Linguistics. Vol. 2: A Diachronic View. Washington, DC: Georgetown University Press, 1984. Pp. 259. $19.95 US." Canadian Modern Language Review 44, nr 1 (grudzień 1987): 177–79. http://dx.doi.org/10.3138/cmlr.44.1.177.

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Fujihara, M., S. Nishiyama i S. Hasegawa. "Effects of agars on determination of potency of polymyxin B sulfate by the agar plate diffusion method." Antimicrobial Agents and Chemotherapy 38, nr 11 (1.11.1994): 2665–67. http://dx.doi.org/10.1128/aac.38.11.2665.

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Zen Achmad Redho Nugraha, Dhany Okawa, Rahmat Giri Anshori, Hasna Prawestry i Tuti Alawiyah. "Identifikasi Kadar Rhodamin B pada Agar-agar dan Sosis". FARMASIS: Jurnal Sains Farmasi 3, nr 2 (30.11.2022): 68–75. http://dx.doi.org/10.36456/farmasis.v3i2.6148.

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Abstract Background: The Government of Indonesia through the Regulation of the Minister of Health Number 033 of 2012 stipulates several dangerous dyes, Rhodamine B is one of the hazardous substances and is prohibited from being used in food products. Objective: This study aims to identify levels of Rhodamine B in snacks. Methods: The research method used was qualitative research using wool yarn and quantitative research using UV-VIS spectrophotometry. Results: in the qualitative test, the agar samples did not contain rhodamine B because there was no color change and in the sausage samples containing rhodamine B, it showed a red color after the wool was washed with water. In the quantitative test, the levels of rhodamine B in agar samples were found to be 39.19 ppm, and in sausage samples as much as 16.23 ppm. Conclusion: The agar and sausage samples used were positive for rhodamine B. Keywords: Rhodamine B, Agar-agar, sausage, qualitative, quantitative Abstrak Latar Belakang: Pemerintah Indonesia melalui peraturan Menteri Kesehatan Nomor 033 Tahun 2012 menetapkan ada beberapa zat pewarna berbahaya, Rhodamin B termasuk salah satu zat berbahaya dan dilarang digunakan pada produk pangan Tujuan: Penelitian ini bertujuan untuk mengidentifikasi kadar Rhodamin B pada jajanan yang beredar. Metode: Metode penelitian yang dilakukan pada penelitian kualitatif dilakukan dengan menggunakan benang wol dan penelitian kuantitatif dengan Spektrofotometri UV-VIS. Hasil: pada uji kualitatif sampel agar-agar tidak mengandung rhodamin B karena tidak adanya perubahan warna dan pada sampel sosis mengandung rhodamin B dengan ditunjukannya warna kemerahan setelah benang wol dicuci dnegan air. Pada uji kuantitatif, kadar rhodamin B pada sampel agar ditemukan sebanyak 39,19 ppm, dan pada sampel sosis sebanyak 16,23 ppm. Kesimpulan: Sampel agar-agar dan sosis yang digunakan positif mengandung rhodamin B. Kata Kunci: Rhodamin B, Agar-agar, sosis, kualitatif, kuantitatif

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Rozprawy doktorskie na temat "AGARD-B"

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Gebelein, Katja [Verfasser], Susanne [Akademischer Betreuer] Kneist, Arndt [Akademischer Betreuer] Güntsch i Nicole B. [Akademischer Betreuer] Arweiler. "In-vitro-Studie zur antimikrobiellen Wirkung von Mundspüllösungen im Agar-Hemmhoftest / Katja Gebelein. Gutachter: Susanne Kneist ; Arndt Güntsch ; Nicole B. Arweiler". Jena : Thüringer Universitäts- und Landesbibliothek Jena, 2013. http://d-nb.info/1036873188/34.

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Kurade, Rajan Bharat. "Control of shock-induced vortex breakdown in Transonic regime on a slender delta wing body configuration using blowing". Thesis, 2019. https://etd.iisc.ac.in/handle/2005/5121.

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Modern high speed aircrafts feature highly swept and low aspect ratio wings, mainly to increase the critical Mach number and reduce the wave drag. A special case of swept wings is wings with triangular planform commonly known as delta wings. The delta wings provide advantageous aerodynamic characteristics such as small lift curve slope, non-linear lift and a higher stalling angle due to it’s ability to form two counter rotating leading edge vortices. These vortices are strong and are a source of high energy which enables higher suction on the lee-ward side of wing and results into an increase in lift. However, as the angle of attack is increased beyond certain value, these vortices are affected by changes in the flow behaviour, which causes them to become unstable and breakdown into an incoherent form. This angle of attack is termed as critical angle of attack, beyond which the organised vortical structures are disintegrated leading to loss in lift and wing stall, a phenomenon called as vortex breakdown (VB). The vortex breakdown is detrimental to the aerodynamic characteristics of the wing and can cause instability of the aircraft. Due to this adverse effect, it is important to understand the behaviour of such flows. The behaviour of the flow over slender delta wings under transonic conditions is highly complex. With the occurrence of a number of shocks in the flow, vortex breakdown is abrupt and the overall behaviour is quite different to that for subsonic flow. In fact, the critical angle of attack is significantly lower in transonic speed range compared to low speeds due to interaction of vortex and cross flow/normal shock. The lower critical angle of attack in transonic flow regimes puts serious limitation on overall flight envelope for high speed aircraft. To consider this, the flow over a AGARD-B configuration having a delta wing of 60◦ sweep angle with sharp leading edge, is investigated experimentally in the transonic Mach number range of 0.8 to 0.95 around the critical angle of attack in the range of 10◦ to 15◦. The experimental results are complimented with numerical simulations performed at Mach number of 0.85 at selected angles of attack. Also, a detailed experimental investigation is carried out on a pneumatic control to delay the vortex breakdown. This thesis presents an experimental study of vortical flows and vortex breakdown over an AGARD-B configuration in transonic Mach number regime. The effect of vortex breakdown on overall aerodynamic characteristics and the variation of critical angle of attack with free stream Mach number is determined. The experimental aerodynamic data of AGARD-B is validated with the literature. Few test cases at free stream Mach number of 0.85, are analysed with numerical steady state simulations performed using High Resolution Flow Solver for Unstructured meshes (HiFUN). The numerical simulation results are validated with the present experimental data. A brief literature review of the control strategies adapted for delaying the vortex breakdown is presented. The literature suggests that ‘along the core blowing’ or ‘spanwise blowing’ method for control are found to be effective especially in subsonic Mach number regime. However, evaluation of these methods in transonic Mach number regime is found to be limited in literature. Also, the basic knowledge of a jet interacting with the transonic cross flow and it’s effect on the oncoming transonic flow is found to be limited in literature hence an experimental study of the sonic and supersonic jet interaction with transonic cross flow is carried out on a flat plate. An empirical correlation is suggested to predict the stream wise pressure variation ahead and behind the round jet injection on a flat plate, for a range of transonic Mach numbers, jet exit conditions and momentum ratios. A judicious selection of jet exit condition is made based on these experimental results and is implemented on AGARD-B configuration. The numerical simulations are utilised to evaluate the effectiveness of ‘along the core blowing’ on the vortex breakdown for AGARD-B configuration for few test conditions. Numerical study reveal a number of spanwise shocks present on the leeward side of wing for a baseline case and with the control case. Implementation of the control jet injection seems to modify the shock shape and reduces the severity of the vortex breakdown and improves the suction on leeward side of the wing. The jet used for the ‘along the core blowing’ requires very low levels of blowing (≈ 0.026%) and significantly enhances the lift to drag ratio by about 17 % near critical angle of attack. A detailed experiments are carried out to understand the effect of ‘spanwise’ and ‘along the core blowing’, control jet exit conditions, the control jet injection location etc. using force and moment measurement, pressure sensitive paint technique and unsteady pressure measurements. Analysis of pressure sensitive paint images, unsteady pressure fluctuations is used to identify the vortex breakdown phenomenon and effect of jet injection and is compared with baseline case. The span wise control jet injection from very close to the apex and tangentially along the leeward surface of the wing is found to be enhancing the lift to drag ratio by 4 to 9% depending upon the free stream Mach number. Also, the critical angle of attack is found to be pushed by approximately 2◦ due to the ‘spanwise blowing’ as compared to the baseline case. The primary and secondary vortex are found to be energized due to the span wise control jet injection. It is felt that the oncoming vortex entrains the fluid from the control jet and eventually enhances the axial velocity of vortex core which helps in pushing the vortex breakdown location of primary vortex by about 40% and that of the secondary vortex by 22%. The overall or cumulative coefficient of fluctuating pressure CPrms measured in the rear portion of the wing shows that the control jet injection reduces the overall coefficient of fluctuating pressure significantly by 20% compared with the baseline case indicating a reduction in the buffet loads. It is also observed that the jet exit condition and thus jet momentum coefficient (J) or coefficient of blowing (Cμ) plays an important role in pushing the vortex breakdown (VB) without disturbing the oncoming vortex significantly, especially since the control jet is injected very close to the apex of the wing. The experience gained from these experiments reveal that, for an effective postponement of vortex breakdown, a sonic jet should be blown either ‘Spanwise (SWB)’ or ‘along the core (ACB)’ from very close to the apex of the delta wing and a moderately low momentum coefficient (J) should be chosen for the injection such that the oncoming flow is minimally disturbed.

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Smith, Amanda R. "Detection and enumeration of sublethally-injured Escherichia coli B-41560 using selective agar overlays". 2012. http://liblink.bsu.edu/uhtbin/catkey/1698824.

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Quality control procedures during food processing may involve either lengthy enrichment steps, precluding enumeration of bacteria in contaminated food, or direct inoculation of food samples onto appropriate selective media for subsequent enumeration. However, sublethally injured bacteria often fail to grow on selective media, enabling them to evade detection and intervention measures and ultimately threaten the health of consumers. This study compares traditional selective and nonselective agar-based overlays versus two commercial systems (Petrifilm and Easygel) for recovery of injured Escherichia coli B-41560, originally an isolate from ground beef. Bacteria were propagated in tryptic soy broth (TSB), ground beef, or infant milk formula (IMF) to a density of 106-108 CFU/mL, and stressed for six minutes either in lactic acid (pH of 4.5) or heat-shocked for 3 min. at 60°C. Samples were pour- plated in basal layers of either tryptic soy agar (TSA), Sorbitol MacConkey (SMAC), or Violet Red Bile (VRB) agar and resuscitated for 4h prior to addition of agar overlays. Other stressed bacteria were plated directly onto the commercial media Petrifilm and Easygel. Our results indicate that the use of selective and nonselective agar overlays for sensitive recovery and accurate enumeration of E. coli B-41560 is dependent on the stress treatment and food system. These data underscore the need to implement food safety measures that address sublethally- injured bacteria such as E. coli O157:H7, without the use of enrichment steps, in order to avoid underestimation of true densities for target pathogens.
Department of Biology

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Agari, Michaela [Verfasser]. "Hyperon production in proton-nucleus collisions at a center-of-mass energy of √sNN=41.6 GeV at HERA-B and design of silicon microstrip detectors for tracking at LHCb / vorgelegt von Michaela Agari". 2006. http://d-nb.info/997899905/34.

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Książki na temat "AGARD-B"

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Opinions in Chaffraix & Agar vs. John B. Lafitte & Co. Gale, Making of Modern Law, 2012.

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Części książek na temat "AGARD-B"

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Sauter, H., i C. J. Paige. "Differentiation of Murine B-Cell Progenitors in Agar Culture: Determination of the Developmental Potential of Clonable Pre-B Cells". W Current Topics in Microbiology and Immunology, 65–74. Berlin, Heidelberg: Springer Berlin Heidelberg, 1987. http://dx.doi.org/10.1007/978-3-642-71851-9_5.

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"Cellobiose polymyxin B colistin (CPC) agar". W Handbook of Culture Media for Food Microbiology, 431–33. Elsevier, 2003. http://dx.doi.org/10.1016/s0079-6352(03)80040-1.

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"Cellobiose polymyxin b colistin (CPC) agar". W Culture Media for Food Microbiology, 278–80. Elsevier, 1995. http://dx.doi.org/10.1016/s0079-6352(05)80028-1.

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"PROTOCOL 07-15.1: Rye B agar". W Laboratory Protocols for Phytophthora Species, redaktor Jean Ristaino, 1–2. The American Phytopathological Society, 2015. http://dx.doi.org/10.1094/9780890544969.07.15.1.

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Souder, Emily, i Sarah S. Long. "Bordetella". W Schlossberg's Clinical Infectious Disease, redaktor Cheston B. Cunha, 869–71. Oxford University Press, 2021. http://dx.doi.org/10.1093/med/9780190888367.003.0126.

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This chapter details the Bordetellae as fastidious, non–carbohydrate-fermenting, tiny, gram-negative coccobacilli that grow aerobically on starch blood agar or synthetic medium. The Bordetellae’s attachment proteins include a 69-kDa outer membrane protein, filamentous hemagglutinin, and fimbriae. Bordetella pertussis is an exclusive human pathogen that causes epidemic pertussis, and it is also the usual cause of endemic and sporadic pertussis. B. parapertussis is an infrequent cause of pertussis in the United States and is genetically more closely aligned with B. bronchiseptica, a common veterinary pathogen that causes upper respiratory tract illnesses in animals. The chapter also examines B. holmesii, which can cause pertussis-like illness in otherwise healthy persons.

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Dolphin, Heather, i Fatima Ahmad. "Bacteriology Diagnostic Methods". W Tutorial Topics in Infection for the Combined Infection Training Programme. Oxford University Press, 2019. http://dx.doi.org/10.1093/oso/9780198801740.003.0015.

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This is summarized in Table 8.1. a) Microscopy— A cell count is performed on sterile fluids and CSF samples using the Neubauer chamber or a similar device. The number of white blood cells (WBC) and red blood cells seen under the microscope are reported as well as the differential WBC count (i.e. the number or percentage of lymphocytes and neutrophils in the sample). A Gram stain is then done and the presence of any organism reported. b) Culture samples are plated onto the appropriate media and streaked out for single colonies as shown. Blood agar is normally used; however, other media are used depending on the site of the specimen, e.g. chocolate agar is used if a fastidious organism is a potential pathogen such as Haemophilus sp.; anaerobic agar for anaerobes; selective agar such as MacConkey can be used on non-sterile specimens to differentiate between the colony types. Plates are incubated for eighteen to forty-eight hours at the correct conditions; most plates being CO2, others at O2 and anaerobically. c) Identification plates are examined for growth. Potentially significant isolates are identified either by MALDI-TOF MS, by API, or other biochemical tests. d) Sensitivities are performed on significant organisms by manual and automated methods. This is summarized in Table 8.2. Selective agar is necessary when isolating pathogens from faeces, although further confirmatory tests are needed. ● Black or colourless colonies on xylose lysine deoxycholate (XLD) or other chromogenic agar plates are tested with oxidase reagent. ● Oxidase negative isolates are identified by MALDI-TOF, API and or biochemically using triple-sugar iron (TSI) tubes. ● Serology is then performed on suspicious isolates and sent to a reference laboratory for confirmation. ● Campylobacter is confirmed by testing grey flat colonies on campylobacter agar with oxidase reagent. Oxidase positive samples are Gram stained and if ‘seagull’-shaped gram-negative bacteria are observed under the microscope, campylobacter is confirmed. The catalase test is a simple biochemical test to differentiate between Staphylococcus species and Streptococcus species, with the use of hydrogen peroxide (H2O2). It tests for the presence of the enzyme catalase which is found in Staphylococcus species.

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Paige, Christopher J., Helena Skarvall, Helmut Sauter i Samuel Magasiny. "Development of B Cell Progenitors in Semisolid Agar Cultures". W Immunological Methods, 221–33. Elsevier, 1985. http://dx.doi.org/10.1016/b978-0-12-442703-7.50021-8.

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Paige, Christopher J., Helmut Sauter i Gillian E. Wu. "Growth of B cell colonies in double-layer agar cultures". W Immunochemical Techniques Part K: In Vitro Models of B and T Cell Functions and Lymphoid Cell Receptors, 257–65. Elsevier, 1987. http://dx.doi.org/10.1016/0076-6879(87)50083-0.

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Paige, Christopher J., i Roland H. Gisler. "Adherent layer-dependent development of B cell progenitors in semisolid agar". W Immunochemical Techniques Part K: In Vitro Models of B and T Cell Functions and Lymphoid Cell Receptors, 251–57. Elsevier, 1987. http://dx.doi.org/10.1016/0076-6879(87)50082-9.

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Streszczenia konferencji na temat "AGARD-B"

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Ren, Xudong, C. Gao, Zijie Zhao, Juntao Xiong, Feng Liu i Shijun Luo. "Boundary-Layer Transition Effects on Aerodynamic Characteristics of AGARD-B Mode". W 50th AIAA Aerospace Sciences Meeting including the New Horizons Forum and Aerospace Exposition. Reston, Virigina: American Institute of Aeronautics and Astronautics, 2012. http://dx.doi.org/10.2514/6.2012-1217.

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Senda, Hiroki, Hideo Sawada, Hiroyuki Okuizumi, Yasufumi Konishi i Shigeru Obayashi. "Aerodynamic Measurements of AGARD-B Model at High Angles of Attack by 1-m Magnetic Suspension and Balance System". W 2018 AIAA Aerospace Sciences Meeting. Reston, Virginia: American Institute of Aeronautics and Astronautics, 2018. http://dx.doi.org/10.2514/6.2018-0302.

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Kryzhko, A. V., L. N. Kuznetsova i A. V. Shirma. "Promising entomopathogenic strain of Bacillus thuringiensis 0428 effective against the Colorado beetle". W РАЦИОНАЛЬНОЕ ИСПОЛЬЗОВАНИЕ ПРИРОДНЫХ РЕСУРСОВ В АГРОЦЕНОЗАХ. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-15.05.2020.14.

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Most of the world produced biopesticides are made by entomopathogenic bacteria B. thuringiensis. So, searching for new strains of it is always necessary. In 2006, the strain B. thuringiensis 0428 was isolated from the caterpillar of the ringed silkworm. The strain 0428 is entomopathogenic against Colorado beetle larvae. The effectiveness of the strain for 5 days was 100%. On beef-extract agar this Gram-positive bacterium formed round or irregular colonies with an average diameter of 6-10 mm. The relief of the colonies is flat; the surface is matte. Colonies of B. thuringiensis 0428 are fast-growing, appearing on the surface of the beef-extract agar on the second or third day at 26-30ºC. The average cell size is 6.48±0.16 (large diameter) and 2.62±0.06 (small diameter) microns. The study of the physiological and biochemical properties of the isolated strain shown that B. thuringiensis 0428 is able to form acetyl-methyl-carbinol and lecithinase. B. thuringiensis 0428 is not able to form ureases or pigments, as well as to use citrates and galactose. But it is able to use sucrose, glucose, mannose, and salicin as a source of carbon. The strain 0428 has proteolytic activity. The strain is capable of synthesizing an insecticidal crystalline protein Cry1A and β-exotoxin. All these characteristics allow us to identify the isolated entomopathogenic strain 0428 as B. thuringiensis var. thuringiensis.

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Günzler, W. A., B. Wolf i L. Flohé. "CHARACTERIZATION OF RECOMBINANT HUMAN SINGLE-CHAIN LOW MOLECULAR WEIGHT UROKINASE (RE-SC-LUK)". W XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643602.

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RE-SC-LUK obtained from recomoinant b. con Bacteria showed a molecular mass similar to that of recombinant two-chain LUK (RE-TC-LUK) as judged from SDS-PAGE. By “Western“ blot analysis immunoreactivity of RE-SC-LUK was observed with monoclonal antibodies directed against the B chain but not with those against the A1 chain of urokinase. N-terminal sequence analysis c RE-SC-LUK showed identity to the A, chain of RE-TC_LUK and provided evidence for its single-chain nature, i.e. integrity of the Lys-Ile bond which is split in TC-UK. In all other respects structural identity of RE-SC-LUK and RE-TC-LUK was demonstrated by fingerprinting of fragments. Similar to recombinant pro-urokinase (RE-SCU-PA), RE-SC-LUK exhibits only marginal amidolytic activity, which is greatly enhanced by treatment with plasmin, but considerable fibrinolytic activity in a fibrin agar plate test.Thus, RE-SC-LUK is characterized as a fragment (residues 136 -411) of RE-SCU-PA, which lacks the “growth factor” and “kringle” domains. Moreover further evidence is provided that a free N-terminus of the B chain is essential for amidolytic but not for fibrinolytic activity of urokinase in more complex systems.

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Lima, Alan Gomes, i Mickie Takagi. "AVALIAÇÃO DO CRESCIMENTO DE HAEMOPHILUS INFLUENZAE TIPO B EM MEIO SÓLIDO ISENTO DE SANGUE DE CAVALO". W I Congresso de Engenharia de Biotecnologia. Revista Multidisciplinar de Educação e Meio Ambiente, 2021. http://dx.doi.org/10.51189/rema/1383.

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Introdução: A bactéria Haemophilus Influenzae tipo b (Hib) apresenta uma grande importância médica em todo mundo, pois, é causadora de diversas doenças, sendo mais agravante a meningite. A capsula polissacarídica que é composto por moléculas de poliribosil-ribitol-fosfato (PRP) é considerada como principal fator de virulência sendo utilizada como antígeno vacinal. Por ser considerada um microrganismo fastidioso, Hib necessita de condições especificas para seu crescimento, por esse motivo o meio solido amplamente utilizado é o Ágar chocolate, onde, adiciona-se sangue de cavalo, carneiro ou coelho em temperatura alta para que liberem compostos fundamentais para seu desenvolvimento. Com a crescente discussão sobre o uso de animais e seus componentes para estudos laboratoriais, a busca por alternativas que diminua o uso destes é extremamente importante, desse modo objetivo do presente estudo é avaliar a viabilidade célular de hib utilizando meio alternativo suplementado com fatores de crescimento NAD e Hemina; preparar o lote de trabalho utilizando o meio alternativo definido; quantificar a concentração de PRP obtida em frasco agitados. Material e métodos: O teste de viabilidade celular foi feito através da contagem do número de colônias viáveis que existem em 1 mL de amostra analisada expressa em UFC/mL; O teste de pureza da colônia foi feito através de Coloração de Gram; O acompanhamento do crescimento celular em meio líquido foi feito através da medida de Densidade Óptica em 540 nm (DO540); a determinação da concentração de PRP foi realizada através do método modificado de Bial (ASHWELL, 1957). Resultados: O meio solido suplementado proposto, proporcionou a manutenção da viabilidade celular em 1,4 x 1010 UFC/mL, muito similar a viabilidade atingida com o agar chocolate. Os ensaios em frascos agitados, em meio líquido apresentaram velocidade máxima específica de crescimento (µmax) de 0,515 h-1 e concentração de PRP na 8ª hora de cultivo de 360,9 mg/L de PRP reproduzindo aos ensaios cujo lote de trabalho fora realizado em agar chocolate. Conclusão: O meio sólido utilizado sem o uso de sangue de cavalo se mostrou eficiente para garantindo a viabilidade celular e a produção de PRP.

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Batsalova, Tsvetelina, Dzhemal Moten, Ivan Butenko, Balik Dzhambazov i Alexander Vasilkov. "BIOLOGICAL AND PHYSICOCHEMICAL PROPERTIES OF GOLD AND IRON NANOPARTICLES PRODUCED BY GREEN SYNTHESIS METHOD". W 22nd SGEM International Multidisciplinary Scientific GeoConference 2022. STEF92 Technology, 2022. http://dx.doi.org/10.5593/sgem2022/6.1/s24.02.

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Gold and iron nanoparticles were generated via environmentally safe metal-vapor synthesis method applying acetone or toluene as organic dispersion medium. Biological properties of the nanoparticles were analyzed by the agar disc diffusion method using Gram-positive and Gram-negative bacteria and via in vitro cytotoxicity assays with different human cell lines. The obtained results revealed distinct biological activity profiles of the studied specimens. Fe nanoparticles (Fe NPs) demonstrated inhibitory effects against both Gram-positive and Gram-negative bacteria. Au nanoparticles (Au NPs) produced in acetone as organic dispersion medium reduced the growth of E. coli, but showed lower activity against the Gram-positive bacterium B. cereus. Au NPs derived from toluene organosol demonstrated the lowest level of antibacterial activity. In vitro analyses with human cells indicated mild cytotoxic effects of Au NPs against all tested cell lines. Fe NPs demonstrated time- and concentration-dependent cytotoxicity against colon adenocarcinoma cells. Iron nanoparticles derived from acetone organosol did not induce negative effect on noncancerous human cells, which indicates a good biocompatibility potential. Their physicochemical properties were characterized by transmission and scanning electron microscopy (TEM, SEM), thermogravimetric analysis (TGA) and X-ray photoelectron spectroscopy (XPS). TEM observations demonstrated that Au NPs and Fe NPs have average sizes of 8.3 nm and 1.8 nm. Characteristics of the photoelectron spectra showed that gold is in the state of Au0, and the spectrum of iron is close in shape to the spectrum of Fe3O4.

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Wulan, Afrida Nawang, i Mochamat Bintoro. "Pengaruh Umur Aplikasi Paclobutrazol dan Dosis Pupuk Boron Terhadap Produksi dan Mutu Benih Jagung Manis (Zea mays saccharata Sturt.)". W Seminar Nasional Semanis Tani Polije 2021. Politeknik Negeri Jember, 2021. http://dx.doi.org/10.25047/agropross.2021.225.

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Untuk menekan pertumbuhan vegetatif tanaman jagung terutama tinggi tanaman agar tanaman tidak mudah rebah, yakni dengan aplikasi paclobutrazol. Selain itu, peningkatan produksi jagung manis juga dapat dilakukan dengan pengelolaan polen guna menjamin ketersediaan polen dan keberhasilan penyerbukan dengan pemberian unsur boron (Lordkaew et al., 2011). Penelitian dilaksanakan di lahan milik PT. Wira Agro Nusantara Sejahtera yang terletak di desa Jl. Pepaya, Lamong, Pare, Kediri selama 4 bulan mulai dari bulan Oktober 2020 sampai dengan Januari 2021. Penelitian ini dilaksanakan dengan menggunakan Rancangan Acak Kelompok secara faktorial yang terdiri dari 2 faktor. Faktor pertama adalah waktu aplikasi paclobutrazol (W) terdiri dari 2 taraf yaitu W1 : 30 HST dan W2 : 40 HST. factor ke dua adalah dosis pupuk boron (B) terdiri dari 3 taraf yaitu B0 : 0 kg/ ha, B1 : 10 kg/ ha, dan B2 : 15 kg/ ha. Hasil penelitian menunjukkan bahwa waktu aplikasi paclobutrazol tidak berpengaruh terhadap hampir seluruh parameter, kecuali tinggi tanaman dan berat 1000 butir. Sedangkan dosis boron menunjukkan pengaruh yang sangat nyata terhadap seluruh parameter produksi, namun tidak memberikan pengaruh yang nyata terhadap daya kecambah dan kecepatan tumbuh benih jagung manis. Interaksi antara waktu aplikasi paclobutrazol dan dosis boron tidak memberikan pengaruh yang nyata pada seluruh parameter, kecuali parameter berat 1000 butir. Tanaman jagung manis yang diberi boron sebanyak 15 kg / ha memiliki umur berbunga paling cepat (52,29 hari setelah tanam dan bobot serbuk sari yang lebih tinggi dibandingkan yang tidak diberi boron. Dosis boron sebanyak 15 kg / ha memberikan pengaruh terbaik terhadap panjang tongkol, diameter tongkol, bobot tongkol, bobot benih per tongkol, jumlah benih per tongkol, bobot 1000 butir, dan produksi benih jagung manis per hektar. Walaupun tidak bebeda nyata dengan pemberian dosis 10 kg/ha.

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Pinheiro, Silviane Bezerra, LIZANDRA STEPHANNY FERNANDES MENESCAL, ALINE STEPHANIE PÉREZ GÓMEZ, KATIA SANTANA CRUZ i JOÃO VICENTE BRAGA DE SOUZA. "INFECÇÃO POR CRYPTOCOCCUS GATTII GENÓTIPO VGII EM PACIENTE NÃO-HIV". W II Congresso Nacional de Microbiologia Clínica On-line. Revista Multidisciplinar em Saúde, 2022. http://dx.doi.org/10.51161/ii-conamic/5800.

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Introdução: A criptococose é uma infecção fúngica de alta incidência mundial que acomete principalmente indivíduos portadores do vírus HIV. Anualmente ocorrem aproximadamente 25.000 casos e 1.800 mortes. Embora seja uma doença oportunista, pacientes não-HIV são infectados e o diagnóstico tardio é um fator negativo no prognóstico clínico. Objetivo: Esse estudo realizou a genotipagem de Cryptococcus sp. que causa infecção em paciente não-HIV do Estado do Amazonas. Metodologia: A amostra de Cryptococcus sp. foi reativada em agar Saboraud, após 24 horas houve a extração do dna e PCR RFLP do gene URA5. Relato de Caso: Paciente sexo feminino, 22 anos, doméstica, residente de casa de madeira no interior do Estado do Amazonas, com histórico de hipertensão arterial sistêmica. Apresentou por 5 meses quadro de cefaleia intensa constante associada a fotofobia evoluindo para náuseas, vômitos, diplopia, paresia dos membros inferiores, amaurose e irritabilidade. Procurou assistência médica, onde foi realizado punção lombar, sendo diagnosticada com neurocriptococose. Foi transferida ao hospital Fundação de Medicina Tropical Doutor Heitor Vieira Dourado para início de tratamento com anfotericina B desoxicolato e fluconazol. Devido alterações visuais, exames oftalmológicos evidenciaram papiledema e aumento da pressão endocraniana sendo necessário a realização de punções lombares de alívio a cada 2 dias. A análise do LCR apresentou cor límpida, proteínas de 109.0 mg/dL, glicose 3.0 mg/dL, exame direto com tinta Nankin evidenciou raras células leveduriformes capsuladas em brotamentos compatíveis com Cryptococcus sp. Após 48 horas de crescimento nos meios Saboraund e Brain Heart Infusion, houve crescimento de colônias de Cryptococcus sp. Análises moleculares por meio da extração de dna e de PCR-RFLP do gene URA5 revelou ser a espécie Cryptococcus gattii de genótipo VGII. Durante internação foi confirmada infecção do trato urinário por Escherichia coli (>100.000 UFC/ml). A paciente evoluiu com anemia, piora progressiva do estado neurológico e posteriormente episódios convulsivos sendo necessário admissão na unidade de terapia intensiva(UTI) e suporte ventilatório. Após cinco dias de tratamento em UTI, a paciente veio a óbito. Conclusão: O diagnóstico tardio e a virulência dessa espécie, que é endemica na região Norte, são fatores que influenciam o prognóstico de pacientes não-HIV.

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Raporty organizacyjne na temat "AGARD-B"

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Eldar, Avigdor, i Donald L. Evans. Streptococcus iniae Infections in Trout and Tilapia: Host-Pathogen Interactions, the Immune Response Toward the Pathogen and Vaccine Formulation. United States Department of Agriculture, grudzień 2000. http://dx.doi.org/10.32747/2000.7575286.bard.

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In Israel and in the U.S., Streptococcus iniae is responsible for considerable losses in various fish species. Poor understanding of its virulence factors and limited know-how-to of vaccine formulation and administration are the main reasons for the limited efficacy of vaccines. Our strategy was that in order to Improve control measures, both aspects should be equally addressed. Our proposal included the following objectives: (i) construction of host-pathogen interaction models; (ii) characterization of virulence factors and immunodominant antigens, with assessment of their relative importance in terms of protection and (iii) genetic identification of virulence factors and genes, with evaluation of the protective effect of recombinant proteins. We have shown that two different serotypes are involved. Their capsular polysaccharides (CPS) were characterized, and proved to play an important role in immune evasion and in other consequences of the infection. This is an innovative finding in fish bacteriology and resembles what, in other fields, has become apparent in the recent years: S. iniae alters surface antigens. By so doing, the pathogen escapes immune destruction. Immunological assays (agar-gel immunodiffusion and antibody titers) confirmed that only limited cross recognition between the two types occurs and that capsular polysaccharides are immunodominant. Vaccination with purified CPS (as an acellular vaccine) results in protection. In vitro and ex-vivo models have allowed us to unravel additional insights of the host-pathogen interactions. S. iniae 173 (type II) produced DNA fragmentation of TMB-8 cells characteristic of cellular necrosis; the same isolate also prevented the development of apoptosis in NCC. This was determined by finding reduced expression of phosphotidylserine (PS) on the outer membrane leaflet of NCC. NCC treated with this isolate had very high levels of cellular necrosis compared to all other isolates. This cellular pathology was confirmed by observing reduced DNA laddering in these same treated cells. Transmission EM also showed characteristic necrotic cellular changes in treated cells. To determine if the (in vitro) PCD/apoptosis protective effects of #173 correlated with any in vivo activity, tilapia were injected IV with #173 and #164 (an Israeli type I strain). Following injection, purified NCC were tested (in vitro) for cytotoxicity against HL-60 target cells. Four significant observations were made : (i) fish injected with #173 had 100-400% increased cytotoxicity compared to #164 (ii) in vivo activation occurred within 5 minutes of injection; (iii) activation occurred only within the peripheral blood compartment; and (iv) the isolate that protected NCC from apoptosis in vitro caused in vivo activation of cytotoxicity. The levels of in vivo cytotoxicity responses are associated with certain pathogens (pathogen associated molecular patterns/PAMP) and with the tissue of origin of NCC. NCC from different tissue (i.e. PBL, anterior kidney, spleen) exist in different states of differentiation. Random amplified polymorphic DNA (RAPD) analysis revealed the "adaptation" of the bacterium to the vaccinated environment, suggesting a "Darwinian-like" evolution of any bacterium. Due to the selective pressure which has occurred in the vaccinated environment, type II strains, able to evade the protective response elicited by the vaccine, have evolved from type I strains. The increased virulence through the appropriation of a novel antigenic composition conforms with pathogenic mechanisms described for other streptococci. Vaccine efficacy was improved: water-in-oil formulations were found effective in inducing protection that lasted for a period of (at least) 6 months. Protection was evaluated by functional tests - the protective effect, and immunological parameters - elicitation of T- and B-cells proliferation. Vaccinated fish were found to be resistant to the disease for (at least) six months; protection was accompanied by activation of the cellular and the humoral branches.

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