Gotowa bibliografia na temat „370.11/5”

Objective: This study was to develop and validate a liquid chromatography–tandem mass spectrometry (LC–MS/MS) for the quantification of lenvatinib (LT) in human plasma.Methods: A simple, sensitive and specific LC–MS/MS method was developed for quantification of LT in human plasma using LTD4 as internal standard (IS). The analytical method consists of liquid–liquid extraction of plasma sample followed by the determination of LT by a LC–MS/MS. The analyte was separated on a Zorbax Eclipse XDB-C18 (150×4.6 mm, 5 μ) column with an isocratic mobile phase of acetontrile:0.1% formic acid (80:20 v/v) at a flow rate of 0.6 mL/minutes. The protonated ions were formed by a turbolon spray in a positive mode were used to detect analyte and IS. The MS/MS detection was made by monitoring the fragmentation of m/z 427.10→370.10 for LT and m/z 430.30→370.10 for IS on a MS.Result: The method was validated with the correlation coefficients of (r2) ≥0.995 over a linear concentration range of 10.20-501.60 pg/mL. This method demonstrated intra- and inter-day precision within 1.06-2.42% and 0.03-0.55% and accuracy within 95.64-100.08% and 97.16-100.07%.Conclusion: This method is suitable and convenient to pharmacokinetics and bioavailability studies for estimation of LT in biological samples by LC–MS/MS.

The purpose of this study was to investigate the effects of feed ingredients with different protein-to-fat ratios on growth, slaughter performance and meat quality of Small-Tail Han lambs. Forty-five Small-Tail Han lambs (♂) (BW = 34.00 ± 2.5 kg, age = 120 ± 9 d) were randomly divided into groups with three different experimental treatments: (1) PF 5, with the ratio of protein to fat (CP:EE) of 50 to 5; (2) PF10, CP: EE = 50:10; (3) PF20, CP: EE = 50:20. Each treatment group had 15 lambs, and each sheep was a repeat. This experiment lasted for 65 days, with feed intake recorded daily, and animals being weighed on days 0, 30, and 65. At the conclusion of the experiment, eight lambs from each group were slaughtered to assess slaughter performance and meat quality. The results showed that the average daily gain (ADG) of the three groups were 315.27, 370.15 and 319.42 g/d, respectively. The PF10 group had the highest ADG (370.15 g) (p < 0.05). Forestomach weights (1216.88 g) of the PF10 group were significantly higher than those of the other groups (p < 0.05). There were no differences (p > 0.05) in fat percentages in various parts of body among treatments. Feed conversion of the PF10 group was higher (p < 0.05) than that of PF 5 and PF 20 groups. Furthermore, the PF10 group had a higher (p > 0.05) carcass weight and slaughter rate and there were few differences between the other groups in terms of dry matter intake, meat quality, organ weight, and fat deposition (p > 0.05). The protein–energy supplement with protein-to-fat ratio, PF10 appeared to be more desirable to promote the growth and development in Small-Tail Han Lambs.

Single crystals of Cu(pz)Br2 and Cu(pz)Cl2 were grown by using gel methods with tetramethoxysilane as the gel-forming reagent. Thermal decomposition is interpreted. Crystal data for the bromo complex: monoclinic, space group C2/m with a = 1239.2(3) pm, b = 685.9(2) pm, c = 390.7(3) pm, β = 96,23(5)°. Crystal data for the chloro-complex: monoclinic, space group C2/m with a = 1197.1(3) pm, b = 684.9(3) pm, c = 370.1(3) pm, β = 95.96(5)°. Crystal structure analyses reveal that CuHal2 molecules are bonded by pyrazine to form linear chains. These chains are cross-linked by bridging halogen atoms.

This study aims to determine the quantitative characteristics of the crossbreed Bali cattle with several male breeds such as the Ongole, Limousine, and Simmental breeds. The method used in this research is the observation method in the form of direct observation in the field and measurement of cattle. A total of 30 adult cattle aged 1.5-2 years, consisting of 10 heads each (5 males and 5 females) crosses through Artificial Insemination (AI) of Bali cattle using frozen semen of Ongole breed bulls (POBAL), Limousin (LIMBAL) and Simmental (SIMBAL). The results showed that POBAL cattle had an average body length of 114.4-116.0 cm, chest circumference 179.4-220.0 cm, shoulder height 126.2-143.8 cm, and body weight 407.15-586.84 kg . LIMBAL cattle have an average body length ranging from 129.4-151.2 cm, chest circumference 170.4-191.8 cm, shoulder height 123.0-151.6 cm, and body weight 370.184-457.122 kg. While the average body length for SIMBAL cattle ranges from 159.8-180.0 cm, chest circumference 166.6-219.8 cm, shoulder height 125.8-154.1 cm, and body weight 356.662-584.678 kg.

Background: Sepsis is the leading cause of death in children worldwide. Early recognition and treatment are essential for preventing progression to lethal outcomes. CRP and Complete Blood Count (CBC) are the initial preferred tests to distinguish between bacterial and viral infections. Specific early diagnostic markers are still missing. Aim: To investigate diagnostic value of Neutrophil–Lymphocyte Ratio (NLR), Mean Platelet Volume (MPV) and Platelet–MPV ratio (PLT/MPV) to distinguish sepsis/bacteremia and viral infection. Methods: We conducted a retrospective data analysis of case records of 115 children from 1 month to 5 years of age. All cases were divided into two groups—sepsis/bacteremia (n = 68) and viral (n = 47) patients, and further subdivided according to the time of arrival into early or late (≤12 or 12–48 h post the onset of fever, respectively). Analysis of CBC and CRP results was performed. NLR and PLT/MPV were calculated. Results: Sepsis/bacteremia group demonstrated higher absolute platelets count (370.15 ± 134.65 × 109/L versus 288.91 ± 107.14 × 109/L; p = 0.001), NLR (2.69 ± 2.03 versus 1.83 ± 1.70; p = 0.006), and PLT/MPV (41.42 ± 15.86 versus 33.45 ± 17.97; p = 0.001). PLT/MPV was increased in early arrival sepsis/bacteremia infants (42.70 ± 8.57 versus 31.01 ± 8.21; p = 0.008). NLR and MPV were significantly lower in infants (≤12 months) with viral infection on late arrival (1.16 ± 1.06 versus 1.90 ± 1.25, p = 0.025 for NLR and 8.94 ± 0.95fl versus 9.44 ± 0.85fl, p = 0.046 for MPV). Conclusion: Together with standard blood biomarkers, such as CRP, neutrophils, or platelets count, PLT/MPV is a promising biomarker for clinical practice to help discriminate between viral disease or sepsis/bacteremia in all children, especially in early onset of symptoms. NLR and MPV could support exclusion of sepsis/bacteremia in late arrival cases.

Nutraceutical cranberry powder extract (CBPE) has distinct polyphenols inhibiting colon cancer growth and proliferation. However, its oral therapeutic efficacy is hindered because of its low permeability. This study aims to formulate chitosan surface-modified PLGA nanoparticles (CS-PLGA NPs) for encapsulating CBPE and modulating its release rate, permeation, cell targeting, and, therefore, its cytotoxicity. A full 23 factorial design is employed to scrutinize the effect of lactide/glycolide ratio, PLGA weight, and stabilizer concentrations on entrapment efficiency percentage (EE%), particle size (PS), polydispersity index (PDI), and zeta potential (ZP). The optimum formula (F4) shows spherical particles with a relatively high EE% (72.30 ± 2.86%), an appropriate size of 370.10 ± 10.31 nm, PDI; 0.398 ± 0.001, and ZP; −5.40 ± 0.21 mV. Alongside the ATR-FTIR outcomes, the chitosan surface-modified formula (CS-F4) demonstrates a significant increase in particle size (417.67 ± 6.77 nm) and a shift from negative to positive zeta potential (+21.63 ± 2.46 mV), confirming the efficiency of surface modification with chitosan. The intestinal permeability of F4 and CS-F4 is significantly increased by 2.19- and 3.10-fold, respectively, compared to the CBPE solution, with the permeability coefficient (Papp) being 2.05 × 10−4 cm/min and 2.91 × 10−4 cm/min, for F4 and CS-F4, respectively, compared to the CBPE solution, 9.36 × 10−5 cm/min. Moreover, CS-F4 evidences significant caspase-3 protein level expression stimulation and significant inhibition of vascular endothelial growth factor (VEGF) and signal transducer and activator of transcription-3 (STAT-3) protein expression levels, confirming the superiority of CS-F4 for targeting HT-29 cells. Briefly, CS-PLGA NPs could be regarded as a prosperous delivery system of CBPE with enhanced permeation, cell targeting, and antitumor efficacy.

Nowadays, to obtain high and stable yields of agricultural crops, in addition to the creation and introduction of new high-yielding varieties, the use of various plant protection products, growth stimulants and biopreparations, the effective use of mineral fertilizers is a necessary agricultural technique. The purpose of this work was to study the influence of various doses and forms of nitrogen fertilizers on the dynamics of the accumulation of raw mass of corn plants, crop structure, yield, as well as the removal and normative consumption of nutrients on different salinization backgrounds in the Shaulder irrigation massif of Turkestan region. The obtained results showed that the best indicators were established in a slightly saline background than in an average saline one. On average, on a slightly saline background, the yield of corn grain, depending on the forms of nitrogen fertilizers at a dose of 80 kg / ha, varied in the range of 13.2-14.9 t/ha. At the same time, the yield gain from the use of various forms of fertilizers at the same doses of nitrogen amounted to 21.6-37.4 %. Against a medium-saline background, the option with the use of ammonium nitrate (variant 5) with an addition of 47.7 % nitrogen fertilizer to the phosphorus-potassium background. Regression models were obtained that accurately reflect the dependences of the grain yield and the elements of the corn crop structure on the total effect of nitrogen fertilizer (x1) and the degree of soil salinity (x2): Y = 18.429 + 0.2656x10.5 - 37.0995x2 with a high coefficient of determination - R2 = 0.954. On a slightly saline background, nitrogen removal by the corn grain harvest with the corresponding amount of by-products according to the experimental options varied from 282.9 kg/ha in the P80K80 option, to 370.1 kg/ha in the P80K80 + N120 option (ammonium sulfate), phosphorus – from 121.4 kg/ha in the P80K80 + N80 option (urea) to 160.7 kg/ha in the P80K80 + N80 option (ammonium nitrate), potassium – from 325.2 kg/ha in the P80K80 + N80 option (urea) to 413.7 kg/ha in the P80K80 + N80 option (ammonium sulfate). On a medium-saline background, nitrogen removal was in the range of 175.3–223.8 kg/ha, phosphorus - 67.4-89.6 kg/ha, potassium – 178.5-217.3 kg/ha. The utilization rate of nitrogen from fertilizers varied significantly depending on the doses and forms of nitrogen fertilizers and the degree of soil salinity.

Abstract Introduction Several commonly prescribed hypnotics may worsen respiratory function, especially in the elderly. Lemborexant (LEM) is a competitive dual-orexin-receptor-antagonist approved to treat adults with insomnia. In Study E2006-A001-113 (NCT04647383), LEM demonstrated respiratory safety in participants with untreated moderate-to-severe chronic obstructive pulmonary disease (COPD). This post hoc analysis evaluated sleep variables from this study. Methods This double-blind, placebo (PBO)-controlled, crossover study enrolled adults (45–90y) with untreated moderate or severe COPD, assessed by Global Initiative for Obstructive Lung Disease spirometry recommendations (apnea hypopnea index &lt; 15 was allowed). Participants were randomized to LEM 10mg (LEM10) or PBO in two 8-night treatment periods (separated by ≥14d). Latency to persistent sleep (LPS), sleep efficiency, wake after sleep onset (WASO), and total-sleep-time (TST) were assessed using in-laboratory polysomnography on Days 1 (D1) and 8 (D8). Results The analysis set comprised 30 adults with moderate (n=25) or severe (n=5) COPD (mean [SD] age, 69.2 [6.3]y; 70.0% female). Six (20%) participants had a medical history of insomnia. Least squares mean (LSM; standard error [SE]) LPS was significantly shorter (improved) with LEM10 compared with PBO on D1 (LEM10, 30.9 [9.0] min; PBO, 51.5 [9.0] min; P&lt; 0.01) and D8 (LEM10, 27.0 [7.0] min; PBO, 46.6 [7.0] min; P&lt; 0.001). LSM (SE) sleep efficiency was significantly higher (improved) with LEM10 compared with PBO on D1 (LEM10, 81.0% [2.6%]; PBO, 66.6% [2.6%]; P&lt; 0.0001) and D8 (LEM10, 77.1% [2.9%]; PBO, 69.4% [2.9%]; P&lt; 0.0001). LSM (SE) WASO was significantly lower (improved) with LEM10 compared with PBO on D1 (LEM10, 68.2 [9.6] min; PBO, 114.6 [9.6] min; P&lt; 0.0001) and D8 (LEM10, 90.4 [9.9] min; PBO, 106.1 [9.9] min; P&lt; 0.0001). LSM (SE) TST was significantly longer (improved) with LEM10 compared with PBO on D1 (LEM10, 388.9 [12.4] min; PBO, 319.5 [12.4] min; P&lt; 0.0001) and D8 (LEM10, 370.1 [13.7] min; PBO, 332.9 [13.7] min; P&lt; 0.0001). LEM was well-tolerated. Conclusion In participants with untreated moderate-to-severe COPD, most of whom did not have a medical history of insomnia, LEM improved sleep onset, sleep maintenance, and TST compared with PBO, suggesting LEM may be a potential treatment option for patients with COPD and insomnia. Support (if any) Eisai, Inc.

Abstract. Atmospheric airborne measurements of CO2 are very well-suited to estimate the time varying distribution of carbon sources and sinks at the regional scale. We present here an analysis of two cross-European airborne campaigns that have been carried out on 23–26 May 2001 (CAATER 1) and 2–3 October 2002 (CAATER 2) over Western Europe. The area covered during CAATER 1 (respectively CAATER 2) was comprised between longitude 4° W to 14° E and latitude 44° N to 52° N (respectively longitude 1° E to 17° E and latitude 46° N to 52° N). High precision in-situ CO2, CO and Radon 222 measurements have been recorded. Flasks samples have been collected during both campaigns to cross-validate the in-situ data. During CAATER 1 (respectively CAATER 2), the mean CO2 concentration was 370.1±4 ppm (respectively 371.7±5 ppm). A HYSPLIT backtrajectories analysis shows that during CAATER 1, dominant winds were blowing from the north-west. In the planetary boundary layer (PBL) airmasses got contaminated over Benelux and Western Germany by pollution from these high urbanized areas, reaching about 380 ppm. Air masses passing over rural areas are depleted in CO2 because of the photosynthesis activity of the land cover vegetation, as low as 355 ppm. During CAATER 2, the backtrajectory analysis shows that airmasses were distributed among the 4 sectors. Airmasses got enriched in CO2 and CO when passing above polluted spots in Germany but also in Poland, as these countries are known to hold part of the most polluting plants based on coal consumption, the so-called "dirty thirty" from WWF. Simultaneous measurements of in-situ CO2 and CO combined to backtrajectories helped us to discriminate the role of fossil fuel emissions from over CO2 sources. The ΔCO/ΔCO2 ratios (R2=0.33 to 0.88, slopes=2.42 to 10.37), calculated for polluted airmasses originating from different countries/regions, matched quite well national inventories, showing that the airborne measurements can help to identify the role of fossil fuel sources even several days/hundreds of kms further in the PBL. CO2 observations have been compared to surrounding ground stations measurements, confirming that the stations located near the ground (ex. CBW, WES, HUN) are representative of the local scale, while those located in the free troposphere (FT) are representative of atmospheric CO2 on a regional scale of a few hundred kilometers (ex. CMN). Stations located several 100 km away measure CO2 concentrations different from a few ppm, indicating the existence of a gradient of a few ppm in the free troposphere. Observations at stations located on top of small mountains (ex. SCH, PUY) match or not the airborne data whether they sample air from the FT or air coming up from the valley. Finally, the analysis of the CO2 vertical variability conducted on the 14 profiles recorded per campaign shows that is at least 5 to 8 times higher in the PBL (4 ppm and 5.7 ppm for CAATER 1 and CAATER 2, respectively) than in the FT (0.5 ppm and 1.1 ppm for CAATER 1 and CAATER 2, respectively). The CO2 jump between the PBL and the FT equals 3.7 ppm for the first campaign and −0.3 ppm for the second campaign. A very striking zonal CO2 gradient of about 11 ppm could be observed in the mid-troposphere during CAATER 2, with higher concentrations in the West than in the East. This gradient could originate from differences in atmospheric mixing, ground emission rates or a earlier beginning of the Fall in the west. More airborne campaigns are currently under analysis in the framework of the CARBOEUROPE-IP project to better assess the role of these different hypothesis. In a companion paper (Xueref-Remy et al., 2010), a comparison of vertical profiles from observations and several modeling frameworks is conducted for both campaigns. An attempt to calculate CO2 fluxes during CAATER 1 using CO2 and Radon-222 observations and modeling tools is also carried out.

Abstract. Atmospheric airborne measurements of CO2 are very well suited for estimating the time-varying distribution of carbon sources and sinks at the regional scale due to the large geographical area covered over a short time. We present here an analysis of two cross-European airborne campaigns carried out on 23–26 May 2001 (CAATER-1) and 2–3 October 2002 (CAATER-2) over Western Europe. The area covered during CAATER-1 and CAATER-2 was 4° W to 14° E long; 44° N to 52° N lat and 1° E to 17° E long; 46° N to 52° N lat respectively. High precision in situ CO2, CO and Radon 222 measurements were recorded. Flask samples were collected during both campaigns to cross-validate the in situ data. During CAATER-1 and CAATER-2, the mean CO2 concentration was 370.1 ± 4.0 (1-σ standard deviation) ppm and 371.7 ± 5.0 (1-σ) ppm respectively. A HYSPLIT back-trajectories analysis shows that during CAATER 1, northwesterly winds prevailed. In the planetary boundary layer (PBL) air masses became contaminated over Benelux and Western Germany by emissions from these highly urbanized areas, reaching about 380 ppm. Air masses passing over rural areas were depleted in CO2 because of the photosynthesis activity of the vegetation, with observations as low as 355 ppm. During CAATER-2, the back-trajectory analysis showed that air masses were distributed among the 4 sectors. Air masses were enriched in CO2 and CO over anthropogenic emission spots in Germany but also in Poland, as these countries have part of the most CO2-emitting coal-based plants in Europe. Simultaneous measurements of in situ CO2 and CO combined with back-trajectories helped us to distinguish between fossil fuel emissions and other CO2 sources. The ΔCO/ΔCO2 ratios (R2 = 0.33 to 0.88, slopes = 2.42 to 10.37), calculated for anthropogenic-influenced air masses over different countries/regions matched national inventories quite well, showing that airborne measurements can help to identify the origin of fossil fuel emissions in the PBL even when distanced by several days/hundreds of kms from their sources. We have compared airborne CO2 observations to nearby ground station measurements and thereby, confirmed that measurements taken in the lower few meters of the PBL (low-level ground stations) are representative of the local scale, while those located in the free troposphere (FT) (moutain stations) are representative of atmospheric CO2 regionally on a scale of a few hundred kilometers. Stations located several 100 km away from each other differ from a few ppm in their measurements indicating the existence of a gradient within the free troposphere. Observations at stations located on top of small mountains may match the airborne data if the sampled air comes from the FT rather than coming up from the valley. Finally, the analysis of the CO2 vertical variability conducted on the 14 profiles recorded in each campaign shows a variability at least 5 to 8 times higher in the PBL (the 1-σ standard deviation associated to the CO2 mean of all profiles within the PBL is 4.0 ppm and 5.7 ppm for CAATER-1 and CAATER-2, respectively) than in the FT (within the FT, 1-σ is 0.5 ppm and 1.1 ppm for CAATER-1 and CAATER-2, respectively). The CO2 jump between the PBL and the FT equals 3.7 ppm for the first campaign and −0.3 ppm for the second campaign. A very striking zonal CO2 gradient of about 11 ppm was observed in the mid-PBL during CAATER-2, with higher concentrations in the west than in the east. This gradient may originate from differences in atmospheric mixing, ground emission rates or Autumn's earlier start in the west. More airborne campaigns are currently under analysis in the framework of the CARBOEUROPE-IP project to better assess the likelihood of these different hypotheses. In a companion paper (Xueref-Remy et al., 2011, Part 2), a comparison of vertical profiles from observations and several modeling frameworks was conducted for both campaigns.

Introduction: Parkinson’s disease (PD) is the second most prevalent neurodegenerative disease in the world. Its incidence increases with advancing age. Therefore, in Brazil, a country in transition of age structure, it is relevant to assess the progression of hospitalizations and hospital costs for PD over the years. Objective: Describe the progression of hospitalizations and hospital costs for PD in Brazilian’s public health system, SUS, between 2008-2020. Design and setting: Descriptive ecological observational study made in Brazil, Salvador – BA. Methods: Data from hospitalization and hospital costs were collected from DATASUS in the 5 Brazilian regions. Statistical analysis was based on measures of dispersion and central tendency. Results: Between 2008-2020, 11,565 admissions for PD were notified. The highest numbers of hospitalizations corresponded to the Southeast region (annual average = 370.1), while the smallest to the North region (annual average = 28.4). Regarding the high expenses resulting from hospitalizations, it was observed that the Southeast obtained higher costs with hospital services (annual average = 1,417,716.8), while the North had the lowest (annual average = 18,611.01). Conclusion: Southeast region stood out for having the highest numbers in costs and hospitalizations, the opposite of what happened in North. Brazilian regional disparities, especially regarding to demographic density, HDI, socioeconomic development and access to health care, may explain these demographically uneven.