Articoli di riviste sul tema "Wv 345"

Abstract Mast cells are thought to be important in the cause of allergic diseases, but the role of mast cells in the airway remodeling of chronic long-term asthma remains controversial. In this study, using the mast cell “nock in” strategy to get the mast cell-deficient WBB6F1-W/Wv mice (W/Wv) selectively reconstituted bone marrow-cultured mast cells (BMCMCs) from normal congenic wild-type mice (+/+) (+/+ BMCMCs→W/Wv mice), mice were employed to study the roles of mast cell in a murine asthma model. The mice were sensitized with ovalbumin (OVA) and challenged with OVA for 14 weeks. The mice were assessed airway reactivity by PenH. The lungs and trachea were stained with H-E for pathologic alteration, toluidine blue for mast cell, congo red for eosinophil, PAS for goblet cell and Masson’s-trichrome for fibrosis. Mast cells were not observed in the lungs of control and OVA-sensitized and challenged W/WV mice, but OVA-sensitization and challenge increased mast cell number in +/+ mice and +/+BMCMCs→W/Wv mice. OVA-sensitization and challenge could enhance airway reactivity, pathologic alteration, eosinophil infiltration, goblet cell hyperplasia and fibrosis in +/+ mice. The pathologic changes of OVA treated W/Wv mice were less than that of +/+ mice, but +/+BMCMCs→W/Wv mice could restore the reaction to OVA-sensitization and challenge of +/+ mice. These results indicate that the mast cell play an important role in the OVA-induced chronic murine asthma model.

Abstract. Retrievals of vertical profiles of key atmospheric gases provide a critical long-term record from ground-based Fourier transform infrared (FTIR) solar absorption measurements. However, the characterization of the retrieved vertical profile structure can be difficult to validate, especially for gases with large vertical gradients and spatial–temporal variability such as water vapor. In this work, we evaluate the accuracy of the most common water vapor isotope (H216O, hereafter WV) FTIR retrievals in the lower and upper troposphere–lower stratosphere. Coincident high-quality vertically resolved WV profile measurements obtained from 2010 to 2016 with balloon-borne NOAA frost point hygrometers (FPHs) are used as reference to evaluate the performance of the retrieved profiles at two sites: Boulder (BLD), Colorado, and at the mountaintop observatory of Mauna Loa (MLO), Hawaii. For a meaningful comparison, the spatial–temporal variability has been investigated. We present results of comparisons among FTIR retrievals with unsmoothed and smoothed FPH profiles to assess WV vertical gradients. Additionally, we evaluate the quantitative impact of different a priori profiles in the retrieval of WV. An orthogonal linear regression analysis shows the best correlation among tropospheric layers using ERA-Interim (ERA-I) a priori profiles and biases are lower for unsmoothed comparisons. In Boulder, we found a negative bias of 0.02±1.9 % (r=0.95) for the 1.5–3 km layer. A larger negative bias of 11.1±3.5 % (r=0.97) was found in the lower free troposphere layer of 3–5 km attributed to rapid vertical change of WV, which is not always captured by the retrievals. The bias improves in the 5–7.5 km layer (1.0±5.3 %, r=0.94). The bias remains at about 13 % for layers above 7.5 km but below 13.5 km. At MLO the spatial mismatch is significantly larger due to the launch of the sonde being farther from the FTIR location. Nevertheless, we estimate a negative bias of 5.9±4.6 % (r=0.93) for the 3.5–5.5 km layer and 9.9±3.7 % (r=0.93) for the 5.5–7.5 km layer, and we measure positive biases of 6.2±3.6 % (r=0.95) for the 7.5–10 km layer and 12.6 % and greater values above 10 km. The agreement for the first layer is significantly better at BLD because the air masses are similar for both FTIR and FPH. Furthermore, for the first time we study the influence of different WV a priori profiles in the retrieval of selected gas profiles. Using NDACC standard retrievals we present results for hydrogen cyanide (HCN), carbon monoxide (CO), and ethane (C2H6) by taking NOAA FPH profiles as the ground truth and evaluating the impact of other WV profiles. We show that the effect is minor for C2H6 (bias <0.5 % for all WV sources) among all vertical layers. However, for HCN we found significant biases between 6 % for layers close to the surface and 2 % for the upper troposphere depending on the WV profile source. The best results (reduced bias and precision and r values closer to unity) are always found for pre-retrieved WV. Therefore, we recommend first retrieving WV to use in subsequent retrieval of gases.

A utilização de diferentes materiais filtrantes tem sido alvo de pesquisas no emprego da ecotecnologia tipo wetland construído no tratamento de águas residuárias a fim de melhorar a eficiência de remoção de poluentes. Neste sentido, o presente estudo objetivou a identificação das potencialidades da utilização do biocarvão como meio filtrante em um wetland construído de escoamento vertical tipo sistema francês (WV-SF), empregado no tratamento de esgoto bruto. O módulo estudado (3,10 m² de área superficial) operou ao longo de 7 meses com uma carga hidráulica média de 0,37 m.d-1, com alternância de 3,5 dias de alimentação e 3,5 dias em repouso. As eficiências globais de remoção de poluentes promovidas pelo WV-SF foram de 73%, 85%, 56%, 78% e 87% para DQO, DBO₅,₂₀ Ptotal, NTK e SST, respectivamente. A condição em que foi operado o WV-SF representa uma redução em 60% do requerimento de área superficial de um clássico wetland Sistema Francês de dois estágios. Desta forma, o biocarvão utilizado como material filtrante neste estudo se mostrou bastante eficaz e promissor para a remoção de contaminantes de águas residuárias por meio dos sistemas wetlands construídos; além de ser um material originário de um resíduo de usina termelétrica. Palavras-chave: Tratamento de esgoto bruto. Wetland construído. Biocarvão. Reaproveitamento de resíduos.

Abstract The normal skin and other tissues of adult genetically mast cell- deficient WBB6F1-W/Wv or WCB6F1-Sl/Sld mice contain less than 1.0% the number of mast cells present in the corresponding tissues of the congenic normal (+/+) mice. We previously reported that mature dermal mast cells developed locally in the skin of W/Wv, but not Sl/Sld, mice at sites of chronic idiopathic dermatitis. We now report that the repeated application of phorbol 12-myristate 13-acetate (PMA) to the ear skin of either W/Wv or +/+ mice induces both dermatitis and a striking and dose-dependent increase in the number of dermal mast cells. The number of dermal mast cells at sites treated for 6 weeks with 5 micrograms PMA, three times per week, was 39 +/- 7/mm2 and 305 +/- 34/mm2 for W/Wv and +/+ mice, respectively; the corresponding values for vehicle-treated skin were 1.5 +/- 1.0/mm2 and 145 +/- 8/mm2, respectively. The PMA-induced dermal mast cells in W/Wv mice appeared mature by morphology, stained with the heparin-binding fluorescent dye, berberine sulfate, and were competent to express IgE-dependent passive cutaneous anaphylaxis responses. The development of mast cells was a local, not systemic, effect of PMA treatment. PMA treatment also induced dermatitis in both WCB6F1-Sl/Sld and +/+ mice, but was associated with increased numbers of dermal mast cells only in the WCB6F1(-)+/+ mice. PMA treatment had no detectable effect on the ability of bone marrow-derived cultured mast cells to survive in the skin of Sl/Sld mice. These findings establish a convenient model system for analyzing factors associated with the development of endogenous populations of mast cells in genetically mast cell-deficient W/Wv mice.

The normal skin and other tissues of adult genetically mast cell- deficient WBB6F1-W/Wv or WCB6F1-Sl/Sld mice contain less than 1.0% the number of mast cells present in the corresponding tissues of the congenic normal (+/+) mice. We previously reported that mature dermal mast cells developed locally in the skin of W/Wv, but not Sl/Sld, mice at sites of chronic idiopathic dermatitis. We now report that the repeated application of phorbol 12-myristate 13-acetate (PMA) to the ear skin of either W/Wv or +/+ mice induces both dermatitis and a striking and dose-dependent increase in the number of dermal mast cells. The number of dermal mast cells at sites treated for 6 weeks with 5 micrograms PMA, three times per week, was 39 +/- 7/mm2 and 305 +/- 34/mm2 for W/Wv and +/+ mice, respectively; the corresponding values for vehicle-treated skin were 1.5 +/- 1.0/mm2 and 145 +/- 8/mm2, respectively. The PMA-induced dermal mast cells in W/Wv mice appeared mature by morphology, stained with the heparin-binding fluorescent dye, berberine sulfate, and were competent to express IgE-dependent passive cutaneous anaphylaxis responses. The development of mast cells was a local, not systemic, effect of PMA treatment. PMA treatment also induced dermatitis in both WCB6F1-Sl/Sld and +/+ mice, but was associated with increased numbers of dermal mast cells only in the WCB6F1(-)+/+ mice. PMA treatment had no detectable effect on the ability of bone marrow-derived cultured mast cells to survive in the skin of Sl/Sld mice. These findings establish a convenient model system for analyzing factors associated with the development of endogenous populations of mast cells in genetically mast cell-deficient W/Wv mice.

Abstract Treatments were applied 27 Jul to plots 8 X 8 ft arranged in a RCBD, replicated 4 times. Granular materials were applied with a shaker jar. Liquid treatments were applied in 1 liter of water per plot with a sprinkling can. The plot area was irrigated after the last treatment had been applied with about ¼inch water using the golf course system. Water pH to apply was 6.85; to irrigate, about 8.0. Environmental conditions at start (8:30 am) and finish (9:35 am) of treatment applications were, respectively: air temperature, 23° C and 31.5° C; RH, 84% and 60%; overcast (fog) and clear (calm); soil temperatures (surface) 26° C and 32° C, (1 inch) 25.5° C and 29.5° C; soil moist from regular irrigation; soil type, loam; soil particle analysis, 40.7% sand, 46.6% silt, 12.7% clay; organic matter, 11.7%, pH 5.35; CEC, 23.81 meg/100 g; 1/3 bar, 31.29%. Thatch thickness ranged from 0.24 to 0.38 inches for 28 samples over plot area. Grub population was 100% Japanese beetle (pre- and post-treatment); avg no. grubs at treatment was 34.5/ft2 (59.4% mid to late 1st instar, 40.6% early 2nd instar). Efficacy was evaluated at 53 DAT using a cup changer to extract 4 plugs near the center of each plot to a depth of 2-3 inches and counting live larvae in each sample. The no. larvae for the 4 plugs from each plot was averaged to produce a mean for each replicate for statistical analysis.

Lead-free (K0.5Na0.5)NbO3 (KNN) nanopowder with perovskite structure was synthesized by mechanochemical synthesis method successfully. In order to investigate the perovskite phase formation of KNN powders during the mechanochemical synthesis process, we set up three milling routes with different transmission ratios (Wv/Wp) acting on the mixed starting powders of Na2CO3, K2CO3 and Nb2O5 . KNN perovskite phase was not detected by XRD under the low transmission ratios -2 with the shock power of 273 W. When the transmission ratios was increased to -2.75 with the shock power of 683.4 W, KNN perovskite phase was formed after milling for 22 h. Based on these results, it has been found, that in shock mode process (SMP), that higher shock power is helpful for the formation KNN perovskite phase. The critical shock power P critical and critical weight-normalized cumulative shock energy [Formula: see text] in KNN system are discussed. Compared with SMP, when Wv/Wp = -3.5, in friction mode process (FMP), KNN perovskite phase was formed after milling for 5 h. It implies that FMP benefit the formation of KNN perovskite phase. The morphology of KNN powder was observed by transmission electron microscopy (TEM).

Introduction With the onset of the COVID-19 pandemic, elective surgeries were decreased and associated with worsened outcomes. Trauma outcomes have been varied, and rural traumas have not been solely analyzed. This study investigated outcomes related to COVID-19 infection at a rural Level I trauma center in West Virginia (WV). Methods A retrospective analysis of trauma outcomes of patients aged 18 years and older from March 30, 2020, until December 21, 2021, presenting to a Level I trauma center in WV was conducted. Data was collected from the John Michael Moore Trauma Center trauma registry. Mortality, complications, injury characteristics, comorbidities, and demographics were examined. Results Out of 2,118 patients tested for the novel coronavirus, 61 (1.7%) were positive (COV+). There was no significant increase in mortality regarding COVID-19 infection. COV+ patients had an increased length of stay (median 5.2 vs. 3.6, p=0.015) and intubation rates (21.3% vs. 12.5%, p=0.070), but fewer days on a ventilator (median, 2 vs. 3, p=0.012). COV+ patients were more likely to be discharged to rehabilitation centers, skilled nursing facilities, or long-term acute care hospitals (44.3% vs 34.5%), and less often routine or home measures (42.6% vs. 58.7%) (p=0.015). Conclusion This single retrospective study found increased rates of length of stay and intubation rates with no increased rates of mortality or complications in COV+ trauma patients compared to non-COV+ trauma patients. Further research is needed to validate and characterize the impact of COVID-19 for the entirety of the state.

Introduction The COVID-19 pandemic began in West Virginia (WV) in March 2020 at the end of the seasonal increase in influenza virus infections. Diagnostic and surveillance testing for influenza continued throughout the pandemic because these viruses cause similar diseases and the effects of SARS-CoV-2 on seasonal influenza patterns were unpredictable. Methods Positivity rates for SARS-CoV-2 and influenza viruses were studied using test results obtained from samples collected across an academic health system from July 2018 through June 2022. Trends in COVID-19 variants were assessed using data obtained from national and local databases. Data were analyzed to detect differences in and correlations between SARS-CoV-2 and influenza positivity rates. Results Test positivity rates were higher for the 602,109 SARS-CoV-2 tests than the 186,563 influenza tests performed during the study period (13.7% vs. 3.5%, p<0.0001). SARS-CoV-2 test positivity rose and fell with the emergence of SARS-CoV-2 variants of differing infectivity, being highest when the omicron variant predominated. Significant negative correlations between the number of SARS-CoV-2 and influenza positive tests were seen throughout stages of the pandemic. The normally expected influenza season did not occur during winter 2020 through spring 2021, and there were essentially no influenza cases during the delta surge. A more typical pattern of seasonal influenza was observed in late 2021 to early 2022 during the omicron surge. Conclusion There was an inverse correlation between SARS-CoV-2 and influenza test positivity rates observed throughout several COVID-19 surges in WV. The reasons for these correlations are unclear.

This study provides the basis for implementing a continuous treatment system for wastewater containing a pesticide mixture formed by atrazine, iprodione, and chlorpyrifos. Two fungal strains (Verticilium sp. H5 and Metacordyceps sp. H12) isolated from a biomixture of a biopurification system were able to remove different pesticide concentrations (10 to 50 mg L−1) efficiently from the liquid medium; however, the half-life of the pesticides was reduced and characterized by a T1/2 of 5.4 to 9.2 d for atrazine, 3.7 to 5.8 d for iprodione, and 2.6 to 2.9 d for chlorpyrifos using the fungal consortium. The immobilization of the fungal consortium in alginate bead was effective, with the highest pesticide removal observed using an inoculum concentration of 30% wv−1. The packed-bed reactor with the immobilized fungal consortium, which was operated in the continuous mode at different flow rates (30, 60, and 90 mL h−1), required approximately 10 d to achieve removal efficiency (atrazine: 59%; iprodione: 96%; chlorpyrifos: ~85%). The bioreactor was sensitive to flow rate fluctuations but was able to recover performance quickly. The pesticide metabolites hydroxyatrazine, 3,5-dichloroaniline, and 3,5,6-trichloro-2-pyridinol were produced, and a slight accumulation of 3,5,6-trichloro-2-pyridinol was observed. Nevertheless, reactor removal efficiency was maintained until the study ended (60 d).

BackgroundFatigue in RA has a multi-causal pathway. It is recommended that it should be measured in all RA studies using a validated instrument. Fatigue in elderly patients could have a different perception than in younger patients, identifying the associated factors could be a key to the management of this complex symptom.ObjectivesTo compare fatigue and its associated factors in young and elderly patients with RA from two university hospitals.MethodsA cross-sectional analysis was performed in 167 RA patients diagnosed according to the 2010 ACR-EULAR criteria. Patients were divided into two groups based on age (≥60 and <60) for comparative purposes. Fatigue was assessed using 4 instruments: the Bristol Rheumatoid Arthritis Fatigue Multidimensional Questionnaire (BRAF-MDQ), the fatigue subscale of the Short-form 36 survey (fatigue-SF36), the Visual Analogic scale of fatigue (VASf) and the Functional Assessment of chronic illness Therapy Fatigue Scale (FACIT-F). To compare the mean of fatigue between groups we used a T-Test.To determine in each group of patients (young and elderly patients) the relationship between the 4 subscales of fatigue (assessed by BRAF-MDQ) and the other variables (DAS28, CPR, ESR, hemoglobin, vitamin D, HAQ, RAID [Rheumatoid Arthritis Impact of Disease], SF36, Hospital Anxiety and depression Scale [HAD] and Brief Pain Inventory) a Spearman correlation was performed. A value of p < 0.05 was accepted as statistically significant.ResultsA total of 167 patients were included, 81 (48.5%) young and 86 (51.5%) elderly patients. We found fatigue (using 4 instruments) has not significant differences in young and elderly patients (Table 1). In young and elderly patients, physical, living, cognitive and emotional fatigue were correlated to RAID, SF36, HAD and pain but they were not associated to CRP, ESR, hemoglobin and vitamin D. In young patients, all dimensions of fatigue were associated with DAS28. Furthermore, in elderly patients we found a relationship between physical (p-value 0.044) and living fatigue (p-value 0.012) with DAS28, nevertheless cognitive and emotional fatigue (p-value 0.078 and 0.079 respectively) were not related.Table 1.Scores of the Fatigue Questionnaires used to assess fatigue in young and elderly patients with RA.Young Mean (SD)Elderly Mean (SD)p-valueFACIT-F36.5 (12.5)35.9 (11.7)0.2963VAS-F4.3 (2.8)3.8 (2.8)0.119SF36-Fatigue50.9 (23.9)51.0 (21.9)0.628BRAF-MDQ Physical9.2 (6.2)8.5 (6.3)0.4670 Living5.3 (5.6)4.2 (4.6)0.1446 Cognitive3.3 (3.8)4.2 (4.6)0.2932 Emotional3.5 (3.5)2.6 (2.7)0.0932ConclusionIn young and elderly patients, all dimensions of fatigue appear to be related with subjective but not with objective variables. In young patients, all dimensions of fatigue were associated with DAS28 but in elderly patients only physical and living fatigue were correlate to disease activity. These results could indicate that it is important to evaluate fatigue in a multidimensional perspective in elderly patients.References[1]Belza BL, Henke CJ, Yelin EH, Epstein WV, Gilliss CL. Correlates of fatigue in older adults with rheumatoid arthritis. Nurs Res. 1993 Mar-Apr;42(2):93-9.[2]Hewlett S, Dures E, Almeida C. Measures of fatigue: Bristol Rheumatoid Arthritis Fatigue MultiDimensional Questionnaire (BRAF MDQ), Bristol Rheumatoid Arthritis Fatigue Numerical Rating Scales (BRAF NRS) for severity, effect, and coping, Chalder Fatigue Questionnaire (CFQ), Checklist Individual Strength (CIS20R and CIS8R), Fatigue Severity Scale (FSS), Functional Assessment Chronic Illness Therapy (Fatigue) (FACIT-F), Multi-Dimensional Assessment of Fatigue (MAF), Multi-Dimensional Fatigue Inventory (MFI), Pediatric Quality Of Life (PedsQL) Multi-Dimensional Fatigue Scale, Profile of Fatigue (ProF), Short Form 36 Vitality Subscale (SF36 VT), and Visual Analog Scales (VAS). Arthritis Care Res (Hoboken). 2011 Nov;63 Suppl 11:S263-86.Disclosure of InterestsNone declared

Background According to the National Institute on Drug Abuse, West Virginia has the highest age-adjusted rate of drug overdose deaths involving opioids. To combat this crisis, comprehensive drug counseling support services were established within the Cabell Huntington Hospital (CHH) system in October 2018 in Huntington, WV, USA. The purpose of this study was to investigate whether these services significantly reduced the number of trauma patients with a positive urine drug screen (UDS) seen at CHH. Methods The trauma registry at CHH was used to obtain data on trauma patients with positive UDS from January 2017 to October 2019, which was divided into groups before and after October 2018. Exclusion criteria were any patients who were prescribed the drug. The percentages of the total number of positive drug screens within each group were calculated, and a t-test analysis was performed to determine the P values. Results 345 trauma patients with positive UDS were selected. Results showed that there was an overall decrease in the rate of nonprescribed use after October 2018 of benzodiazepines (18.1%-11.5%), cocaine (19.5%-15.3%), opioids (19.1%-12.3%), and oxycodone (10.2%-4.6%). However, none of these changes were statistically significant. There was an increase in the rate of nonprescribed use of amphetamine (20.0%-23.8%) and methamphetamine (14.4%-33.8%). Discussion Our hypothesis is that the support systems are relatively new, and may need more time to identify and intervene on patients before a statistically significant effect on drug abuse rates in our region can be seen.

ABSTRACT The study aimed to assess the effect of progressive neutralization on the antimicrobial properties against bacteria and yeasts of wood vinegar obtained from the pyrolysis of Eucalyptus urograndis (clone I144) wood. Wood samples were carbonized at a heating rate of 0.9 °C min-1 until a final temperature of 450 °C, totalizing 8 hours of carbonization. The raw pyrolysis liquids were left to settle, and the aqueous fraction was separated. Then, the aqueous fraction (raw wood vinegar - WV) was purified to yield the WV. WV samples were collected and neutralized from pH 2.5 until 7.5 (2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, and 7.5, by adding NaOH solution. Through the broth microdilution method, the antimicrobial effect of the neutralized samples at each pH was assessed on Pseudomonas aeruginosa (ATCC 27853), Salmonella enteritidis (ATCC 13076), Staphylococcus aureus (ATCC 25923), Streptococcus agalactiae (CEPA CLINICA), and Candida albicans (ATCC 10231). The minimum inhibitory concentration (MIC) and minimum bactericidal (and fungicidal) concentrations were determined through in vitro technics. Results were subjected to logarithmic regression analysis, and statistical models were fitted for each microorganism in the assessed pH range; as pH increased, a progressive decrease in the CIM increased, demanding higher concentrations of WV to inhibit microbial growth. The more resistant strains were S. aureus and S. agalactiae, which required an increase in WV concentration from a minimum of 6.25% at pH 2.5 to reaching 50% at pH 6.0. When at pH 7.0, both strains were not inhibited even at 50% (the highest concentration evaluated in the study). In contrast, C. albicans proved to be the most sensitive strain, starting from 3.12% EP at pH 2.5 and requiring only 25% for inhibition at pH 7.0. The behavior of P. aeruginosa and S. enteritidis followed the pattern of C. albicans, differing only at pH 7.0, where they required 50% of EP. As observed, even at neutral and slightly alkaline pH, the inhibitory activity of EP on microbial growth was maintained to some extent. Nevertheless, even when neutral and slightly alkaline pH values are reached, the inhibitory activity remains at a certain level. Higher pH values of the WV were associated with lower antimicrobial activity. However, its activity remained even at neutral and slightly alkaline pH values.

Introduction: West Virginia exhibits pervasive cardiovascular disease (CVD) that may relate to a combination of ancestry and shared environment in families, including vulnerabilities related to diet, physical activity and tobacco use. Coronary Artery Risk Detection in Appalachian Communities (CARDIAC) is a school-based child risk factor screening program that has evaluated over 90,000 WV fifth graders in the past 20 years. Reverse Cascade screening for Familial Hypercholesterolemia (FH) has been difficult with the CARDIAC population. The WVU CTSI Integrated Data Repository (IDR) includes over 2 million records. Hypothesis: Linkage of child CARDIAC data to parent IDR data will allow new information discovery to inform management of CVD. Methods: We used direct demographic data linkage via Oracle with Soundex conversion of names, in the IDR, to find parents of the CARDIAC participants. Data was analyzed in the VMWare SSL environment. Results: 4759 children have a parent(s) identified. 959 mothers and 524 fathers have an LDL level from IDR. Race, BMI and gender was recorded from CARDIAC. 6.8 % of children, 40% of mothers and 44.8% of fathers have an abnormal LDL level >130 mg/dl in IDR. Positive predictive value of the abnormal child lipid level (≥130 mg/dl) is 17% for some parent (56/325) to be abnormal. 4 parents had LDL >190 mg/dl with child > 160, indicating likely FH in the pair (2.7% of pairs or 1 in 371 pairs). Conclusion: Formation of a virtual cohort of CARDIAC children and parents allows Virtual Reverse Cascade Screening to find FH. This project highlights the importance of familial tendency to hyperlipidemia that can aid detection of early lipid abnormality and cardiovascular risk in children and their young parents to promote wellness and potentially avoid early coronary artery disease. We are constructing a virtual longitudinal cohort to study CVD in WV as a part of a Learning Health System in which data management is at the forefront of healthcare improvement.

As maloclusões são classificadas como o terceiro maior problema de saúde bucal no mundo, perdendo apenas para cárie e doença periodontal. A mordida cruzada posterior é definida como a relação anormal vestíbulo-lingual de um ou mais dentes da maxila, com um ou mais dentes da mandíbula, quando os arcos dentários estão em relação cêntrica, podendo ser uni ou bilateral. Objetiva-se Realizar uma revisão de literatura sobre a mordida cruzada posterior. Foi feita uma seleção de artigos científicos a partir das bases de dados Lilacs e Scielo utilizando os descritores “Mordida Cruzada” e “Diagnóstico de Mordida Cruzada”. Foram incluídos trabalhos publicados entre 2000 a 2018. Dos 694 artigos encontrados e delimitados pelos critérios inclusivos, foram selecionados 49 artigos como amostra, que apresentaram a temática elencada para a pesquisa e que foram discutidos nos seguintes tópicos: a) Epidemiologia; b) Etiologia; c) Diagnóstico; d) Tratamento. As causas da mordida cruzada posterior são multifatoriais e seu diagnóstico precoce é fundamental uma vez que, a literatura mostra resultados satisfatórios, através de medidas interceptativas com um prognostico favorável quando o tratamento ocorre precocemente. O tratamento da mordida cruzada posterior de origem funcional, por contato prematuro em dentes decíduos, dentoalveolar e esquelético consiste, respectivamente, em desgaste seletivo, expansão dentoalveolar e disjunção maxilar.Descritores: Ortodontia; Aparelhos Ortodônticos; Má Oclusão; Odontopediatria.ReferênciasAlmeida MR, Pereira ALP, Almeida RR, Almeida-Pedrin RR, Silva Filho OG. Prevalência de má oclusão em crianças de 7 a 12 anos de idade. Dental Press J Orthod. 2011;16(4):123-31.Janson G, Barros SEC, Simão TM, Freitas MR. Variáveis relevantes no tratamento da má oclusão de Classe II. Rev Dental Press Ortodon Ortop Facial. 2009;14(1):149-57.Sousa RV, Pinto-Monteiro AKA, Martins CC, Granville-Garcia AF, Paiva SM. Maloclusion and socioeconomic indicators in primay dentition. Braz Oral Res. 2014;28(1):54-60.Carvalho CM, Carvalho LFPC, Forte FDS, Aragão MS, Costa LJ. Prevalência de mordida aberta anterior em crianças de 3 a 5 anos em Cabedelo/PB e relação com hábitos bucais deletérios. Pesq Bras Odontoped Clin Integr. 2009;9(2):205-10.Sousa RV, Clementino MA, Gomes MC, Martins CC, Graville-Garcia AF, Paiva SM. Maloclusion and quality of life in Brazilian preschoolers. Eur J Oral Sci. 2014;122(3):223-29.Bittencourt MA, Machado AW. Prevalência de má oclusão em crianças entre 6 e 10 anos: um panorama brasileiro. Dental Press J Orthod. 2010;15(6):113-22.Stahl F, Grabowski R, Gaebel M, Kundt G. Relationship between occlusal findings and orofacial myofunctional status in primary and mixed dentition. Part II: Prevalence or orofacial dysfunctions. J Orofac Orthop. 2007;68(2):74-90. Grabowski R, Stahl F, GaebeL M, Kundt G. 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