Bibliografie tematiche / VBNC

Letteratura scientifica selezionata sul tema "VBNC"

Autore: Grafiati
Pubblicato: 4 giugno 2021
Ultima modifica: 11 marzo 2023

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Articoli di riviste sul tema "VBNC"

1

Highmore, Callum J., Jennifer C. Warner, Steve D. Rothwell, Sandra A. Wilks e C. William Keevil. "Viable-but-NonculturableListeria monocytogenesandSalmonella entericaSerovar Thompson Induced by Chlorine Stress Remain Infectious". mBio 9, n. 2 (17 aprile 2018): e00540-18. http://dx.doi.org/10.1128/mbio.00540-18.

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ABSTRACTThe microbiological safety of fresh produce is monitored almost exclusively by culture-based detection methods. However, bacterial food-borne pathogens are known to enter a viable-but-nonculturable (VBNC) state in response to environmental stresses such as chlorine, which is commonly used for fresh produce decontamination. Here, complete VBNC induction of green fluorescent protein-taggedListeria monocytogenesandSalmonella entericaserovar Thompson was achieved by exposure to 12 and 3 ppm chlorine, respectively. The pathogens were subjected to chlorine washing following incubation on spinach leaves. Culture data revealed that total viableL. monocytogenesandSalmonellaThompson populations became VBNC by 50 and 100 ppm chlorine, respectively, while enumeration by direct viable counting found that chlorine caused a <1-log reduction in viability. The pathogenicity of chlorine-induced VBNCL. monocytogenesandSalmonellaThompson was assessed by usingCaenorhabditis elegans. Ingestion of VBNC pathogens byC. elegansresulted in a significant life span reduction (P= 0.0064 andP< 0.0001), and no significant difference between the life span reductions caused by the VBNC and culturableL. monocytogenestreatments was observed.L. monocytogeneswas visualized beyond the nematode intestinal lumen, indicating resuscitation and cell invasion. These data emphasize the risk that VBNC food-borne pathogens could pose to public health should they continue to go undetected.IMPORTANCEMany bacteria are known to enter a viable-but-nonculturable (VBNC) state in response to environmental stresses. VBNC cells cannot be detected by standard laboratory culture techniques, presenting a problem for the food industry, which uses these techniques to detect pathogen contaminants. This study found that chlorine, a sanitizer commonly used for fresh produce, induces a VBNC state in the food-borne pathogensListeria monocytogenesandSalmonella enterica. It was also found that chlorine is ineffective at killing total populations of the pathogens. A life span reduction was observed inCaenorhabditis elegansthat ingested these VBNC pathogens, with VBNCL. monocytogenesas infectious as its culturable counterpart. These data show that VBNC food-borne pathogens can both be generated and avoid detection by industrial practices while potentially retaining the ability to cause disease.
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2

Ayrapetyan, Mesrop, Tiffany C. Williams e James D. Oliver. "Interspecific Quorum Sensing Mediates the Resuscitation of Viable but Nonculturable Vibrios". Applied and Environmental Microbiology 80, n. 8 (7 febbraio 2014): 2478–83. http://dx.doi.org/10.1128/aem.00080-14.

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ABSTRACTEntry and exit from dormancy are essential survival mechanisms utilized by microorganisms to cope with harsh environments. Many bacteria, including the opportunistic human pathogenVibrio vulnificus, enter a form of dormancy known as the viable but nonculturable (VBNC) state. VBNC cells can resuscitate when suitable conditions arise, yet the molecular mechanisms facilitating resuscitation in most bacteria are not well understood. We discovered that bacterial cell-free supernatants (CFS) can awaken preexisting dormant vibrio populations within oysters and seawater, while CFS from a quorum sensing mutant was unable to produce the same resuscitative effect. Furthermore, the quorum sensing autoinducer AI-2 could induce resuscitation of VBNCV. vulnificus in vitro, and VBNC cells of a mutant unable to produce AI-2 were unable to resuscitate unless the cultures were supplemented with exogenous AI-2. The quorum sensing inhibitor cinnamaldehyde delayed the resuscitation of wild-type VBNC cells, confirming the importance of quorum sensing in resuscitation. By monitoring AI-2 production by VBNC cultures over time, we found quorum sensing signaling to be critical for the natural resuscitation process. This study provides new insights into the molecular mechanisms stimulating VBNC cell exit from dormancy, which has significant implications for microbial ecology and public health.
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Hamabata, Takashi, Mitsutoshi Senoh, Masaaki Iwaki, Ayae Nishiyama, Akihiko Yamamoto e Keigo Shibayama. "Induction and Resuscitation of Viable but Nonculturable Corynebacterium diphtheriae". Microorganisms 9, n. 5 (26 aprile 2021): 927. http://dx.doi.org/10.3390/microorganisms9050927.

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Many pathogenic bacteria, including Escherichia coli and Vibrio cholerae, can become viable but nonculturable (VBNC) following exposure to specific stress conditions. Corynebacterium diphtheriae, a known human pathogen causing diphtheria, has not previously been shown to enter the VBNC state. Here, we report that C. diphtheriae can become VBNC when exposed to low temperatures. Morphological differences in culturable and VBNC C. diphtheriae were examined using scanning electron microscopy. Culturable cells presented with a typical rod-shape, whereas VBNC cells showed a distorted shape with an expanded center. Cells could be transitioned from VBNC to culturable following treatment with catalase. This was further evaluated via RNA sequence-based transcriptomic analysis and reverse-transcription quantitative PCR of culturable, VBNC, and resuscitated VBNC cells following catalase treatment. As expected, many genes showed different behavior by resuscitation. The expression of both the diphtheria toxin and the repressor of diphtheria toxin genes remained largely unchanged under all four conditions (culturable, VBNC, VBNC after the addition of catalase, and resuscitated cells). This is the first study to demonstrate that C. diphtheriae can enter a VBNC state and that it can be rescued from this state via the addition of catalase. This study helps to expand our general understanding of VBNC, the pathogenicity of VBNC C. diphtheriae, and its environmental survival strategy.
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4

Fleischmann, Susanne, Christian Robben, Thomas Alter, Peter Rossmanith e Patrick Mester. "How to Evaluate Non-Growing Cells—Current Strategies for Determining Antimicrobial Resistance of VBNC Bacteria". Antibiotics 10, n. 2 (26 gennaio 2021): 115. http://dx.doi.org/10.3390/antibiotics10020115.

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Thanks to the achievements in sanitation, hygiene practices, and antibiotics, we have considerably improved in our ongoing battle against pathogenic bacteria. However, with our increasing knowledge about the complex bacterial lifestyles and cycles and their plethora of defense mechanisms, it is clear that the fight is far from over. One of these resistance mechanisms that has received increasing attention is the ability to enter a dormancy state termed viable but non-culturable (VBNC). Bacteria that enter the VBNC state, either through unfavorable environmental conditions or through potentially lethal stress, lose their ability to grow on standard enrichment media, but show a drastically increased tolerance against antimicrobials including antibiotics. The inability to utilize traditional culture-based methods represents a considerable experimental hurdle to investigate their increased antimicrobial resistance and impedes the development and evaluation of effective treatments or interventions against bacteria in the VBNC state. Although experimental approaches were developed to detect and quantify VBNCs, only a few have been utilized for antimicrobial resistance screening and this review aims to provide an overview of possible methodological approaches.
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5

Fakruddin, Md, Khanjada Shahnewaj Bin Mannan e Stewart Andrews. "Viable but Nonculturable Bacteria: Food Safety and Public Health Perspective". ISRN Microbiology 2013 (26 settembre 2013): 1–6. http://dx.doi.org/10.1155/2013/703813.

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The viable but nonculturable (VBNC) state is a unique survival strategy of many bacteria in the environment in response to adverse environmental conditions. VBNC bacteria cannot be cultured on routine microbiological media, but they remain viable and retain virulence. The VBNC bacteria can be resuscitated when provided with appropriate conditions. A good number of bacteria including many human pathogens have been reported to enter the VBNC state. Though there have been disputes on the existence of VBNC in the past, extensive molecular studies have resolved most of them, and VBNC has been accepted as a distinct survival state. VBNC pathogenic bacteria are considered a threat to public health and food safety due to their nondetectability through conventional food and water testing methods. A number of disease outbreaks have been reported where VBNC bacteria have been implicated as the causative agent. Further molecular and combinatorial research is needed to tackle the threat posed by VBNC bacteria with regard to public health and food safety.
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6

Wagley, Sariqa, Helen Morcrette, Andrea Kovacs-Simon, Zheng R. Yang, Ann Power, Richard K. Tennant, John Love, Neil Murray, Richard W. Titball e Clive S. Butler. "Bacterial dormancy: A subpopulation of viable but non-culturable cells demonstrates better fitness for revival". PLOS Pathogens 17, n. 1 (13 gennaio 2021): e1009194. http://dx.doi.org/10.1371/journal.ppat.1009194.

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The viable but non culturable (VBNC) state is a condition in which bacterial cells are viable and metabolically active, but resistant to cultivation using a routine growth medium. We investigated the ability of V. parahaemolyticus to form VBNC cells, and to subsequently become resuscitated. The ability to control VBNC cell formation in the laboratory allowed us to selectively isolate VBNC cells using fluorescence activated cell sorting, and to differentiate subpopulations based on their metabolic activity, cell shape and the ability to cause disease in Galleria mellonella. Our results showed that two subpopulations (P1 and P2) of V. parahaemolyticus VBNC cells exist and can remain dormant in the VBNC state for long periods. VBNC subpopulation P2, had a better fitness for survival under stressful conditions and showed 100% revival under favourable conditions. Proteomic analysis of these subpopulations (at two different time points: 12 days (T12) and 50 days (T50) post VBNC) revealed that the proteome of P2 was more similar to that of the starting microcosm culture (T0) than the proteome of P1. Proteins that were significantly up or down-regulated between the different VBNC populations were identified and differentially regulated proteins were assigned into 23 functional groups, the majority being assigned to metabolism functional categories. A lactate dehydrogenase (lldD) protein, responsible for converting lactate to pyruvate, was significantly upregulated in all subpopulations of VBNC cells. Deletion of the lactate dehydrogenase (RIMD2210633:ΔlldD) gene caused cells to enter the VBNC state significantly more quickly compared to the wild-type, and adding lactate to VBNC cells aided their resuscitation and extended the resuscitation window. Addition of pyruvate to the RIMD2210633:ΔlldD strain restored the wild-type VBNC formation profile. This study suggests that lactate dehydrogenase may play a role in regulating the VBNC state.
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Shamloei, Sharareh, Ali Nabavi-Rad, Habibollah Nazem e Abbas Yadegar. "Current Perspectives on Viable but Non-culturable Bacteria in Food Safety and Public Health". Avicenna Journal of Clinical Microbiology and Infection 9, n. 1 (29 marzo 2022): 8–17. http://dx.doi.org/10.34172/ajcmi.2022.02.

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Abstract (sommario):
The viable but non-culturable (VBNC) state is defined as an adaptive mechanism for microorganisms adjusting to stressful conditions. Although VBNC bacteria are alive and metabolically active, they are unable to grow on routine culture media. Nevertheless, the potential capacity of VBNC pathogens to retain virulence activity and further resuscitate into the culturable state in favorable conditions constitutes a major hazard to food safety and public health. Food processing, transformation, and storage, as well as non-thermal techniques, can provoke pathogens toward VBNC induction. The distinct characteristic of VBNC bacteria led to the emergence of novel culture-independent techniques to prevent the misinterpretation of food safety. To deepen our knowledge of the molecular aspect of the VBNC state, several mechanism-oriented studies investigated the metabolic activity of VBNC bacteria and their correlation with different stressful conditions. This review aims to discuss the molecular mechanisms and genomic factors underlying the induction and resuscitation of the VBNC state. The study will further highlight innovative detection methods to provide a comprehensive perspective for future studies in the emerging fields of research concerning VBNC state, food safety, and public health.
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Grey, Brian E., e Todd R. Steck. "The Viable But Nonculturable State ofRalstonia solanacearum May Be Involved in Long-Term Survival and Plant Infection". Applied and Environmental Microbiology 67, n. 9 (1 settembre 2001): 3866–72. http://dx.doi.org/10.1128/aem.67.9.3866-3872.2001.

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ABSTRACT The role of the dormant-like viable but nonculturable (VBNC) condition in the etiology of bacterial infection was examined using a plant system. The plant-pathogenic bacterium Ralstonia solanacearum was first shown to enter into the VBNC state both in response to cupric sulfate when in a saline solution and when placed in autoclaved soil. To determine if the VBNC condition is related to pathogenesis, the physiological status of bacteria recovered from different regions of inoculated tomato plants was determined at different stages of infection. The fraction of in planta bacteria that were VBNC increased during infection and became greater than 99% by the late stage of disease. The possibility that soil-dwelling VBNC bacteria may resuscitate and infect plants was also examined. When tomato seeds were germinated in sterile soil that contained VBNC but no detectable culturable forms of R. solanacearumcells, resuscitation was observed to occur in soil adjacent to plant roots; these resuscitated bacteria were able to infect plants. This is the first report of R. solanacearum entering the VBNC state and of resuscitation of any VBNC plant-pathogenic bacteria and provides evidence that the VBNC state may be involved in explaining the persistent nature of some infections.
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9

Wideman, Nathan E., James D. Oliver, Philip Glen Crandall e Nathan A. Jarvis. "Detection and Potential Virulence of Viable but Non-Culturable (VBNC) Listeria monocytogenes: A Review". Microorganisms 9, n. 1 (19 gennaio 2021): 194. http://dx.doi.org/10.3390/microorganisms9010194.

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The detection, enumeration, and virulence potential of viable but non-culturable (VBNC) pathogens continues to be a topic of discussion. While there is a lack of definitive evidence that VBNC Listeria monocytogenes (Lm) pose a public health risk, recent studies suggest that Lm in its VBNC state remains virulent. VBNC bacteria cannot be enumerated by traditional plating methods, so the results from routine Lm testing may not demonstrate a sample’s true hazard to public health. We suggest that supplementing routine Lm testing methods with methods designed to enumerate VBNC cells may more accurately represent the true level of risk. This review summarizes five methods for enumerating VNBC Lm: Live/Dead BacLightTM staining, ethidium monoazide and propidium monoazide-stained real-time polymerase chain reaction (EMA- and PMA-PCR), direct viable count (DVC), 5-cyano-2,3-ditolyl tetrazolium chloride-4′,6-diamidino-2-phenylindole (CTC-DAPI) double staining, and carboxy-fluorescein diacetate (CDFA) staining. Of these five supplementary methods, the Live/Dead BacLightTM staining and CFDA-DVC staining currently appear to be the most accurate for VBNC Lm enumeration. In addition, the impact of the VBNC state on the virulence of Lm is reviewed. Widespread use of these supplemental methods would provide supporting data to identify the conditions under which Lm can revert from its VBNC state into an actively multiplying state and help identify the environmental triggers that can cause Lm to become virulent. Highlights: Rationale for testing for all viable Listeria (Lm) is presented. Routine environmental sampling and plating methods may miss viable Lm cells. An overview and comparison of available VBNC testing methods is given. There is a need for resuscitation techniques to recover Lm from VBNC. A review of testing results for post VBNC virulence is compared
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Šuster, Katja, e Andrej Cör. "Induction of Viable But Non-Culturable State in Clinically Relevant Staphylococci and Their Detection with Bacteriophage K". Antibiotics 12, n. 2 (2 febbraio 2023): 311. http://dx.doi.org/10.3390/antibiotics12020311.

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Prosthetic joint infections are frequently associated with biofilm formation and the presence of viable but non-culturable (VBNC) bacteria. Conventional sample culturing remains the gold standard for microbiological diagnosis. However, VBNC bacteria lack the ability to grow on routine culture medium, leading to culture-negative results. Bacteriophages are viruses that specifically recognize and infect bacteria. In this study, we wanted to determine if bacteriophages could be used to detect VBNC bacteria. Four staphylococcal strains were cultured for biofilm formation and transferred to low-nutrient media with different gentamycin concentrations for VBNC state induction. VBNC bacteria were confirmed with the BacLightTM viability kit staining. Suspensions of live, dead, and VBNC bacteria were incubated with bacteriophage K and assessed in a qPCR for their detection. The VBNC state was successfully induced 8 to 19 days after incubation under stressful conditions. In total, 6.1 to 23.9% of bacteria were confirmed alive while not growing on conventional culturing media. During the qPCR assay, live bacterial suspensions showed a substantial increase in phage DNA. No detection was observed in dead bacteria or phage non-susceptible E. coli suspensions. However, a reduction in phage DNA in VBNC bacterial suspensions was observed, which confirmed the detection was successful based on the adsorption of phages.
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Più fonti

Tesi sul tema "VBNC"

1

Babic, Darko Andre [Verfasser]. "Induktion und Detektion des VBNC-Zustandes in Pseudomonas aeruginosa / Darko Andre Babic". Wuppertal : Universitätsbibliothek Wuppertal, 2017. http://d-nb.info/1135940487/34.

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2

ZANDRI, GIADA. "Ruolo di forme VBNC di stafilococco presenti in biofilm associati a cateteri endovascolari". Doctoral thesis, Università Politecnica delle Marche, 2009. http://hdl.handle.net/11566/242379.

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3

Alleron, Laëtitia. "Etude de l'état viable non cultivable (VBNC) chez Legionella pneumophila Lens après traitements monochloramine et thermique". Poitiers, 2008. http://theses.edel.univ-poitiers.fr/theses/2008/Alleron-Laetitia/2008-Alleron-Laetitia-These.pdf.

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Legionella pneumophila, l’agent responsable d’une pneumonie sévère atypique chez l’homme, appelée maladie du légionnaire, est ubiquitaire dans les environnements aquatiques. Les traitements généralement appliqués dans les réseaux d’eau afin d’éradiquer cette bactérie sont souvent suivis d’une recolonisation rapide de cette bactérie. Un des facteurs pouvant expliquer cette recolonisation peut être l’état Viable Non Cultivable (VBNC). L’objectif de nos travaux a été d’étudier la survie de L. Pneumophila après des traitements oxydant et thermique et notamment d’étudier l’état VBNC. Dans un premier temps nous avons réalisé un traitement monochloramine et un traitement thermique sur une suspension de légionelles. Nous avons observé que le traitement par 20 mg/L de monochloramine ainsi que celui à 57,5°C, conduisaient à la formation de VBNC chez L. Pneumophila. De plus ces bactéries peuvent persister dans cet état pendant au moins 169 jours après traitement. Dans un second temps, une étude partielle des protéines exprimées à l’état VBNC a été menée. Nous avons montré que l’expression de plusieurs protéines, impliquées dans les voies de production d’énergie, de synthèse protéique et dans la virulence, était augmentée d’un facteur supérieur ou égale à deux par comparaison avec des bactéries ayant subies un stress monochloramine ou une carence en nutriments. Dans un troisième temps, la recherche d’un facteur de ressuscitation Rpf a été entreprise chez L. Pneumophila. Un gène Rpf-like, possédant 33% d’identité et 51% de similarité avec celui de Salmonella, a été découvert. Ce gène a été cloné mais la protéine recombinante obtenue n’a pas conduit à un retour à la cultivabilité des cellules
Legionella pneumophila , the causative agent of an atypical severe pneumonia in human called Legionnaires’ disease, is ubiquitous in aquatic environments. The treatments usually applied to eradicate this bacterium are often followed by a quick new contamination. One of the causes that could explain this colonisation could be the Viable But NonCulturable state (VBNC). The aim of this work was to study the survival of L. Pneumophila after oxidative and heat treatments and then to study the VBNC. First, we have treated Legionella suspension with monochloramine and heat. We have observed that 20 mg/L monochloramine treatment and 57. 5°C treatment lead to the VBNC state. Moreover, these bacteria could persist in this state during at least 169 days. Secondly, we have realised a partial study of Legionella proteins expressed in the VBNC state. Several proteinsexpressions, implicated in energy production, protein synthesis and virulence, were induced more than 2-fold in comparison with bacteria stressed by monochloramine or in starvation. Finally, we have search for a Resuscitation-promoting-factor Rpf in L. Pneumophila genome. An rpf-like gene, showing 33% of identity and 51% similarity with Salmonella rpf was found. This gene has been cloned but the recombinant protein obtained didn’t lead to a recovery of legionella culturability
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Alleron, Laëtitia Frère Jacques. "Etude de l'état viable non cultivable (VBNC) chez Legionella pneumophila Lens après traitements monochloramine et thermique". Poitiers : I-Médias, 2008. http://theses.edel.univ-poitiers.fr/index.php?id-253.

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5

Desneux, Jérémy. "Étude de la survie et de la viabilité de Listeria monocytogenes dans les effluents d’élevages porcins". Thesis, Rennes 1, 2015. http://www.theses.fr/2015REN1S123/document.

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La listériose est une maladie rare mais grave d’origine alimentaire provoquée par Listeria monocytogenes. En raison de sa capacité de survie importante dans les sols, la présence de cette bactérie dans les effluents d’élevages porcins destinés à être épandus constitue un problème de santé publique. L’un des facteurs pouvant expliquer la persistance de L. monocytogenes dans l’environnement est sa capacité à entrer dans un état viable mais non cultivable (VNC). Nos travaux avaient pour objectif, d’une part de suivre le comportement de L. monocytogenes dans les effluents d'élevages porcins (lisier et effluent de lagune) et notamment les formes VNC, et d’autre part d’étudier son adaptation lors de son transfert dans les effluents de lagune et dans le sol. Dans un premier temps, nous avons optimisé les conditions de la qPCR couplée au propidium monoazide (qPCR-PMA) afin d’adapter cette méthode au dénombrement des formes VNC de L. monocytogenes dans le lisier et l’effluent de lagune. Dans un second temps, nous avons comparé par méthode culturale, qPCR-PMA et qPCR, la survie de deux souches de L. monocytogenes RifR de sérogroupes IIb et IVb inoculées dans deux lisiers et dans deux effluents de lagune incubés à 8°C et 20°C. Malgré leur origine et leur sérotype différents, les deux souches ont présenté une survie similaire dans toutes les conditions testées. La survie des deux souches a été affectée par la température (une persistance plus élevée a été observée à 8°C) et par l’origine des effluents. Cette étude a mis en évidence que L. monocytogenes était capable d’entrer dans l’état VNC dans les lisiers et les effluents de lagune indépendamment de la température. Les formes VBNC qui représentaient 83 à 99,8% des bactéries viables après 60 jours d’incubation, sont apparues dès les premières heures de contact avec les effluents. Leur proportion, plus élevée en début d’expérience dans les lisiers que dans les effluents de lagune, était cependant du même ordre de grandeur dans les deux types de matrices après 60 jours. Afin de mieux comprendre l’adaptation de L. monocytogenes lors de son transfert dans l’effluent de lagune et dans le sol, nous avons comparé le transcriptome par la technologie RNA-seq de la souche CIP 110868, isolée d’un lisier, inoculée dans des extraits stériles d’effluent de lagune et de sol. L’analyse du transcriptome a été réalisée à T0 (génome de référence), après 20 minutes et 20 heures d’incubation. L’analyse par enrichissement fonctionnel a révélé des modifications transcriptomiques dès les 20 premières minutes d’incubation dans les deux matrices. Une augmentation du taux de transcrit de gènes impliqués dans le transport de protéines et de sucres a été observée. Le taux de transcrit des gènes contrôlés par le facteur sigmaB est augmenté indiquant la mise en place d’une réponse aux stress osmotiques et thermiques. De plus, l’adaptation de la souche CIP 110868 dans les extraits de sol et d’effluent de lagune s’est accompagnée d’une augmentation au cours du temps des taux de transcrit des gènes impliqués dans la virulence et des gènes sous le contrôle du régulateur prfA
Listeriosis is a rare but serious illness caused by foodborne Listeria monocytogenes. Because of its important survival capacity in the soil, the presence of this bacteria in effluent from pig farms intended to be spread is a public health problem. One factor that may explain the persistence of L. monocytogenes in the environment is its ability to enter a viable but non-culturable state (VNC). Our studies were aimed, firstly to monitor the behavior of L. monocytogenes in effluent from pig farms (manure and lagoon effluent) including VNC forms, and also to study its adaptation at transfer to the lagoon effluent and soil. First, we have optimized the conditions of qPCR coupled with propidium monoazide (qPCR-LDCs) to adapt this method to count VNC forms of L. monocytogenes in the manure and lagoon effluent. Secondly, we compared by cultivation method, qPCR and qPCR-LDCs, the survival of two strains of L. monocytogenes RifR IIb and IVb serogroups manure inoculated in two and two lagoon effluent incubated at 8 ° C and 20 ° C. Despite their origin and different serotype, the two strains showed a similar survival in all conditions tested. The survival of both strains was affected by the temperature (higher persistence was observed at 8 ° C) and the origin of the effluent. This study showed that L. monocytogenes was able to enter the VNC state in manure and lagoon effluent regardless of temperature. VBNC forms which accounted for 83 99.8% of viable bacteria after 60 days of incubation, appeared in the early hours of contact with effluent. Their proportion, higher at the beginning of experience in the manure lagoon in the effluent, however, was of the same order of magnitude in the two types of matrices after 60 days. To better understand the adaptation of L. monocytogenes when transferred into the lagoon effluent and soil, we compared the transcriptome by RNA-Seq technology CIP 110868 strain, isolated from a slurry inoculated in sterile effluent lagoon and extracts of soil. Transcriptome analysis was performed at T0 (reference genome), after 20 minutes and 20 hours of incubation. Functional enrichment analysis revealed transcriptomic changes during the first 20 minutes of incubation in both matrices. An increase in the gene transcript levels involved in the transport of proteins and sugars was observed. The rate of transcribed genes controlled by the sigmaB factor is increased indicating the establishment of a response to osmotic and thermal stress. In addition, the adaptation of the CIP 110868 strain in soil extracts and lagoon effluent was accompanied by an increase in time of transcript levels of genes involved in virulence and gene under the control the prfA regulator
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6

Kortebi, Mounia. "Caractérisation d’une phase de persistance intracellulaire du pathogène Listeria monocytogenes". Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS477/document.

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Listeria monocytogenes est une bactérie pathogène intracellulaire facultative responsable d’une pathologie grave, la listériose. Si de très nombreux travaux ont permis de caractériser les mécanismes de virulence de cette bactérie, il existe peu de données sur les mécanismes conduisant au portage asymptomatique de L. monocytogenes dans les hôtes mammifères. L’un de ces mécanismes pourrait être une phase de persistance intracellulaire. Lors d’infections prolongées de cellules épithéliales humaines en culture, comme des hépatocytes et des cellules de trophoblastes, L. monocytogenes change de mode de vie intracellulaire. Après la phase active de dissémination de cellule en cellule, les bactéries arrêtent de polymériser l’actine et se retrouvent piégées dans des vacuoles à simple membrane marquées par la protéine endosomale LAMP1. L’objectif de ma thèse était de caractériser ces « Listeria-Containing Vacuoles » (LisCVs). Nous avons montré que les LisCVs sont des compartiments acides, partiellement-dégradatifs, marquées par la protéase lysosomale cathépsine D. Leur formation coïncide avec la disparition du facteur de polymérisation d’actine ActA de la surface bactérienne et la capture des bactéries cytosoliques dépourvues d’actine par des membranes cellulaires. Dans ces compartiments, les bactéries entrent en croissance ralentie ; une sous-population résiste aux stress et peut survivre au-delà de trois jours d’infection. L’utilisation de la gentamicine lors du protocole d’infection n’est pas responsable de la formation des LisCVs. Cependant, cet antibiotique permet la sélection des bactéries vacuolaires, en inhibant spécifiquement la croissance des bactéries cytosoliques. La formation des LisCVs n’est pas spécifique des souches de laboratoire. Toutefois l’efficacité du phénomène pourrait diverger selon les séquençotypes des souches de L. monocytogenes. Les bactéries vacuolaires ont la capacité de sortir des vacuoles et de retourner vers un état motile et réplicatif, après le passage des cellules infectées. Lorsque l’expression du gène actA reste inactive, comme dans les mutants ∆actA, des formes de Listeria vacuolaires persistent dans les cellules hôtes dans un état viable mais non cultivable (VBNC). Ces formes VBNC peuvent être transmises au cours des divisions des cellules hôtes. L’ensemble de ces résultats révèle une nouvelle phase de persistance dans le processus infectieux intracellulaire de L. monocytogenes lors des infections prolongées de certaines cellules épithéliales. Cette propriété pourrait contribuer au portage asymptomatique de ce pathogène dans les tissus épithéliaux, allonger la période d'incubation de la listériose, et rendre les bactéries tolérantes à l’antibiothérapie
Listeria monocytogenes is a facultative intracellular pathogenic bacterium responsible for a serious disease, listeriosis. Although much work has been done to characterize the virulence mechanisms of this bacterium, there is little data on the mechanisms leading to the asymptomatic carriage of L. monocytogenes in mammalian hosts. One of these mechanisms could be a phase of intracellular persistence. During prolonged infections of human epithelial cells in culture, such as hepatocytes and trophoblast cells, L. monocytogenes changes its intracellular lifestyle. After the active phase of cell-to-cell spread, the bacteria stop polymerizing actin and become trapped in single-membrane vacuoles labeled with the endosomal protein LAMP1.The aim of my thesis was to characterize these "Listeria-Containing Vacuoles" (LisCVs). We have shown that LisCVs are acidic, partially degradative compartments, labeled by the lysosomal protease cathepsin D. Their formation coincides with the disappearance of actin polymerization factor ActA from the bacterial surface and the capture of actin-free cytosolic bacteria by cell membranes. In these compartments, bacterial growth is slowed; a subpopulation is resistant to stress and can survive beyond three days of infection. The use of gentamicin during the infection protocol is not responsible for the formation of LisCVs. However, this antibiotic allows selection of vacuolar bacteria, by specifically inhibiting the growth of cytosolic bacteria. The formation of LisCVs is not specific to laboratory strains. However, the efficacy of the phenomenon could diverge according to the sequence types of L. monocytogenes strains. Vacuolar bacteria have the ability to exit the vacuoles and return to a motile and replicative state during the subculture of infected cells. When expression of the actA gene remains inactive, as in ΔactA mutants, vacuolar Listeria forms persist in host cells in a viable but non-culturable (VBNC) state. These VBNC forms can be transmitted during host cell divisions. All these results reveal a new phase of persistence in the intracellular infectious process of L. monocytogenes during prolonged infections of a subset of epithelial cells. This property could contribute to asymptomatic carriage of this pathogen in epithelial tissues, extend the incubation period of listeriosis, and make bacteria tolerant to antibiotic therapy
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Tran, Dao. "Identifying risks associated with organic soil amendments: microbial contamination in compost and manure amendments". Thesis, The University of Sydney, 2021. https://hdl.handle.net/2123/27364.

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The application of animal manures to agricultural land as a soil organic amendment has been identified as an important route by which foodborne pathogens can enter the human food chain. Knowledge of the presence and incidence of key foodborne pathogens in manure is a vital first step in the establishment of sound and effective guidelines for management and prevention of contamination by manure. The work described in this thesis attempts to determine the persistence of pathogens when raw manures are directly applied to agricultural soils, and treated under thermophilic composting conditions. Results from this study indicated that the current conditions suggested by typical food safety guidelines are sufficient to reduce the population of enteric bacteria to levels that minimise risks associated with culturable cells in raw manure and finished compost. However, E. coli cells have the potential to enter a viable but non-culturable (VBNC) state and are undetected by culture-based monitoring methods, thus providing a false impression of the innate risk of the product. Prc, bamB and tolA, which are responsible for stabilising the cell membrane, were found to be essential genes required for surviving heat treatment at 55°C. In addition, the presence of relA and oxyR suggested that E. coli may use the VBNC state as an adaptive strategy for long-term survival to withstand multiple stresses, including heat stress. Entering the VBNC state with a strengthen cell envelope may help E. coli to survive prolonged heating during standard composting conditions. Successful resuscitation from the VBNC state was achieved in the presence of cell-free supernatant from actively growing E. coli MG1655. These results underline the importance of considering VBNC cells when evaluating the sanitary effect of the composting process. VBNC cells in composts could facilitate the persistence of pathogens in manure-amended soil and thus pose a risk of microbial contamination of fresh produce.
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Highmore, Callum. "Decontamination of biofilm and VBNC zoonotic pathogens on the salad leaf phylloplane for enhanced food security and safety". Thesis, University of Southampton, 2017. https://eprints.soton.ac.uk/419013/.

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Produce-associated outbreaks of foodborne pathogens such as Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella enterica are rising in prominence among outbreaks of foodborne disease. Testing for foodborne pathogens by the agricultural industry relies heavily on culture-based techniques, excluding detection of viable but nonculturable (VBNC) pathogens. Here, a detection method is used that facilitates the use of qPCR on the complex environmental matrices of soil. Targeting the tir gene of E. coli O157, detection of the pathogen in peat-based compost and sand is achieved to a sensitivity of 10 CFU/g. When applied to pristine soil, 310 copies of the gene were detected. Further analysis using PNA-FISH and cell elongation determined the presence of 205 VBNC E. coli O157 cells per gram of soil sample. Resuscitation of the pathogen was achieved through prolonged enrichment in selective media. Decontamination of fresh produce using chlorine washes was simulated using L. monocytogenes and S. enterica serovar Thompson adhered to spinach leaves, resulting in complete VBNC induction of viable cells following two minutes exposure to 50 ppm and 100 ppm chlorine respectively. The infectivity of these chlorine induced VBNC pathogens was assessed in vivo using Caenorhabditis elegans as an animal model. VBNC L. monocytogenes retained its infectivity and caused a significant lifespan reduction (p=0.0064). Together, these data provide evidence of the presence and induction of VBNC foodborne pathogens throughout the food production chain, and determines that VBNC L. monocytogenes presents a threat to food safety.
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Toni, Jufner Celestino Vaz [UNESP]. "Transições reversíveis de células de leveduras entre estados viáveis, mas não cultiváveis (VBNC) e estados viáveis e cultiváveis". Universidade Estadual Paulista (UNESP), 2015. http://hdl.handle.net/11449/136009.

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Made available in DSpace on 2016-03-07T19:20:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-07-16. Added 1 bitstream(s) on 2016-03-07T19:24:36Z : No. of bitstreams: 1 000855447_20170716.pdf: 923470 bytes, checksum: 64cae1b5d12a5a1a9f32bdebf26d71a1 (MD5) Bitstreams deleted on 2017-07-24T11:34:14Z: 000855447_20170716.pdf,. Added 1 bitstream(s) on 2017-07-24T11:35:19Z : No. of bitstreams: 1 000855447.pdf: 2348425 bytes, checksum: 43653b8cc49553da465fb52374eab833 (MD5)
Diversos estados fisiológicos têm sido descritos para células de levedura durante o cultivo, como células lesionadas, células mortas e células viáveis, mas não cultiváveis (VBNC). No entanto, pouca atenção ainda é dada à formação de células viáveis, mas não cultiváveis em leveduras e seus impactos sobre o processo de fermentação. O estado VBNC, também conhecido como estado de dormência celular pode ser definido como um estado fisiológico em que os micro-organismos não são capazes de crescer em meios bacteriológicos, mas apresentam características de células vivas. Fatores químicos e ambientais têm sido relatados para induzir um estado VBNC, incluindo a falta de nutrientes, temperaturas extremas, concentrações osmóticas e oxigênio. As células de levedura no estado VBNC podem exercer influencias sobre o rendimento de biocombustível gerado no final do processo. As leveduras industriais utilizadas neste estudo foram coletadas em uma usina sucroalcooleira localizada na cidade de Américo Brasiliense-SP-Brasil. Para fornecer evidências da existência de um estado VBNC em leveduras investigou-se a capacidade das células ao entrarem no estado VBNC, aplicando estresse térmico (40°C) e osmótico (30% de sacarose). Populações viáveis foram monitoradas utilizando o corante azul de metileno, e as populações cultiváveis foram identificadas por semeadura em meio de cultura. Todos os procedimentos foram realizados em triplicata, para todas as amostras e os dados amostrais foram submetidos à análise de variância (ANOVA). Após 48 horas no período de tempo em estresse, a comparação entre as células viáveis e as células cultiváveis demonstrou a presença de células viáveis, mas não cultiváveis. Além disso, a remoção do estresse permitiu que as células fossem novamente capazes de crescer, fornecendo evidências da existência de um estado VBNC em leveduras.
Several physiological conditions have been described for yeast cells during cultivation, such as injured cells, dead cells and viable cells, but not culturable (VBNC). However, little attention is still given to the formation of viable cells, but not cultivable in yeast and its impact on the fermentation process. The VBNC state, also known as state of numbness cell can be defined as a physiological condition in which the microorganisms are not able to grow on bacteriological media, but that present characteristics of living cells. Chemical and environmental factors have been reported to induce VBNC state, including a lack of nutrients, temperature extremes, osmotic concentration and oxygen. The yeast cells in the VBNC state may influence the yield of biofuel generated at the end of the process. The yeasts used in this study were collected in a sugarcane mill located in Américo Brasiliense-SP-Brazil. To provide evidence of the existence of a state in yeast VBNC investigated the ability of cells to enter the VBNC state, applying heat stress (40°C) and osmotic (30% sucrose). Viable populations were monitored using the methylene blue dye, and cultivated populations were identified by inoculation in culture. All procedures were performed in triplicate for all samples and sample data were submitted to analysis of variance (ANOVA). After 48 hours the time of stress, the comparison between viable cells and culturable cells showed the presence of viable cells, but not culturable. Moreover, removal of stress the cells were allowed to grow again able, providing evidence for the existence of a VBNC state in yeast.
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Toni, Jufner Celestino Vaz. "Transições reversíveis de células de leveduras entre estados viáveis, mas não cultiváveis (VBNC) e estados viáveis e cultiváveis /". Araraquara, 2015. http://hdl.handle.net/11449/136009.

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Orientador: Cecília Laluce
Banca: Maria Lucia Gonsales da Costa Araujo
Banca: Juliana Audi Giannoni
Resumo: Diversos estados fisiológicos têm sido descritos para células de levedura durante o cultivo, como células lesionadas, células mortas e células viáveis, mas não cultiváveis (VBNC). No entanto, pouca atenção ainda é dada à formação de células viáveis, mas não cultiváveis em leveduras e seus impactos sobre o processo de fermentação. O estado VBNC, também conhecido como estado de "dormência celular" pode ser definido como um estado fisiológico em que os micro-organismos não são capazes de crescer em meios bacteriológicos, mas apresentam características de células vivas. Fatores químicos e ambientais têm sido relatados para induzir um estado VBNC, incluindo a falta de nutrientes, temperaturas extremas, concentrações osmóticas e oxigênio. As células de levedura no estado VBNC podem exercer influencias sobre o rendimento de biocombustível gerado no final do processo. As leveduras industriais utilizadas neste estudo foram coletadas em uma usina sucroalcooleira localizada na cidade de Américo Brasiliense-SP-Brasil. Para fornecer evidências da existência de um estado VBNC em leveduras investigou-se a capacidade das células ao entrarem no estado VBNC, aplicando estresse térmico (40°C) e osmótico (30% de sacarose). Populações viáveis foram monitoradas utilizando o corante azul de metileno, e as populações cultiváveis foram identificadas por semeadura em meio de cultura. Todos os procedimentos foram realizados em triplicata, para todas as amostras e os dados amostrais foram submetidos à análise de variância (ANOVA). Após 48 horas no período de tempo em estresse, a comparação entre as células viáveis e as células cultiváveis demonstrou a presença de células viáveis, mas não cultiváveis. Além disso, a remoção do estresse permitiu que as células fossem novamente capazes de crescer, fornecendo evidências da existência de um estado VBNC em leveduras.
Abstract: Several physiological conditions have been described for yeast cells during cultivation, such as injured cells, dead cells and viable cells, but not culturable (VBNC). However, little attention is still given to the formation of viable cells, but not cultivable in yeast and its impact on the fermentation process. The VBNC state, also known as state of "numbness cell" can be defined as a physiological condition in which the microorganisms are not able to grow on bacteriological media, but that present characteristics of living cells. Chemical and environmental factors have been reported to induce VBNC state, including a lack of nutrients, temperature extremes, osmotic concentration and oxygen. The yeast cells in the VBNC state may influence the yield of biofuel generated at the end of the process. The yeasts used in this study were collected in a sugarcane mill located in Américo Brasiliense-SP-Brazil. To provide evidence of the existence of a state in yeast VBNC investigated the ability of cells to enter the VBNC state, applying heat stress (40°C) and osmotic (30% sucrose). Viable populations were monitored using the methylene blue dye, and cultivated populations were identified by inoculation in culture. All procedures were performed in triplicate for all samples and sample data were submitted to analysis of variance (ANOVA). After 48 hours the time of stress, the comparison between viable cells and culturable cells showed the presence of viable cells, but not culturable. Moreover, removal of stress the cells were allowed to grow again able, providing evidence for the existence of a VBNC state in yeast.
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Libri sul tema "VBNC"

1

United States. Agency for Healthcare Research and Quality., a cura di. Vaginal birth after cesarean (VBAC). [Rockville, Md.]: Agency for Healthcare Research and Quality, 2003.

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United States. Agency for Healthcare Research and Quality, a cura di. Vaginal birth after cesarean (VBAC). [Rockville, Md.]: Agency for Healthcare Research and Quality, 2003.

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Jukelevics, Nicette. VBAC source book and teaching kit. Minneapolis: International Childbirth Education, 2000.

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Artemis speaks: VBAC stories and natural childbirth information. Occidental, Calif., U.S.A: J.R. Brown, 1985.

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Kaufmann, Elizabeth. Vaginal birth after cesarean: The smart woman's guide to VBAC. Alameda, CA: Hunter House Publishers, 1996.

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6

Kaufmann, Elizabeth. Vaginal birth after cesarean: The smart woman's guide to VBAC. Alameda, CA: Hunter House Publishers, 1996.

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Kaufmann, Elizabeth. Vaginal birth after cesarean: The smart woman's guide to VBAC. Alameda, Calif: Hunter House Publishers, 1996.

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8

Kaufmann, Elizabeth. Vaginal birth after cesarean: The smart woman's guide to VBAC. Alameda, CA: Hunter House Publishers, 1996.

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Korte, Diana. The VBAC companion: The expectant mother's guide to vaginal birth after cesarean. Boston, Mass: Harvard Common Press, 1997.

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Open season: A survival guide for natural childbirth and VBAC in the 90s. New York: Bergin & Garvey, 1991.

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Capitoli di libri sul tema "VBNC"

1

Kumain, Sandeep Chand, e Kamal Kumar. "VBNC: Voting Based Noise Classification Framework Using Deep CNN". In Conference Proceedings of ICDLAIR2019, 357–63. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-67187-7_37.

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Wagley, Sariqa. "The Viable but Non-Culturable (VBNC) State in Vibrio Species: Why Studying the VBNC State Now Is More Exciting than Ever". In Advances in Experimental Medicine and Biology, 253–68. Cham: Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-22997-8_13.

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Amano, Fumio. "Differential Resuscitative Effects of Pyruvate and Its Analogs on VBNC (Viable But Nonculturable)Salmonella". In Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria, 1338–45. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2016. http://dx.doi.org/10.1002/9781119004813.ch131.

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Wasson, Cassandra, Albert Kelly, David Ninan e Quy Tran. "Vaginal Birth After Cesarean Section (VBAC)". In Absolute Obstetric Anesthesia Review, 173. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-96980-0_57.

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Qin, Danyang, Yan Zhang, Jingya Ma, Songxiang Yang e Erfu Wang. "Accelerated vBNN–IBS Authentication Scheme for WSN". In Advances in Intelligent Information Hiding and Multimedia Signal Processing, 290–97. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-63859-1_36.

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Spengler, J. "Results From ARGUS About |Vbu|/|Vbc|". In XXIV International Conference on High Energy Physics, 505–10. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-74136-4_30.

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7

Müller-Römer, F. "Das Vorläufer-Breitbandnetz (VBN) und seine Nutzungsmöglichkeiten". In Telecommunications, 256–70. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-95654-6_35.

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Gao, Yun-Bo, Li-Xia Yan, Mao-qing Li e Zhi-Yu Tian. "Research on Movement Authorization Generation of VBTC Based on Timed Automata". In Advances in Natural Computation, Fuzzy Systems and Knowledge Discovery, 596–609. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-70665-4_66.

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Bitter, F., R. Knopp, R. Ochsenkühn, P. Hieber, W. E. Adam, H. J. Biersack e P. Müller. "Nuclear Medicine Image Distribution and Telecommunication through Public Broadband VBN". In Computer Assisted Radiology / Computergestützte Radiologie, 804–10. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-662-00807-2_130.

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Raposo, Vítor, Darijana Antonić, António Casa Nova, Roman Andrzej Lewandowski e Paulo Melo. "An Overview of Measurement Systems and Practices in Healthcare Systems Applied to Person-Centred Care Interventions". In Intelligent Systems for Sustainable Person-Centered Healthcare, 119–43. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-79353-1_7.

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AbstractPerson-centred care (PCC) is an increasing international priority and a shift in health systems orientation and development. Innovative models are required across Europe to prototype healthcare based on health promotion and PCC to improve healthcare quality and costs containment. Regardless of the type of intervention, investments will be required, and it will be essential to demonstrate the value created, comparing consequences and the associated costs. Independent of PCC intervention, we must consider different decision levels and stakeholders in the process. This work aims to focus on a broader perspective of health governance on PCC implementations, considering the need and importance of measurement systems (outcomes and costs) to support and evaluate innovative health service delivery models. It is necessary to have a global view of the entire system considering, from a health governance perspective, the different decision-making levels, the multiple stakeholders and the alignment of their interests. Value-Based Healthcare (VBHC), Value for Money (VfM) and economic evaluation provide concepts, methodologies, and tools that can be used to compare costs and consequences evaluating their impact on society. We need accurate outcomes and costs measurement systems and evaluation tools that can be incorporated in an organizational environment supporting organizational learning and interaction in exchanging knowledge and experience about implementation.
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Atti di convegni sul tema "VBNC"

1

Ali Al Mohamdi, Wijdan, Bahrouz Al-jaff e Sajid AL– Saeedi. "Cultivation and Detection of Viable But Nonculturable (VBNC) Fungi in Soil by Using Yoghurt Whey Infusion Agar (YWIA)". In 2018 International Conference on Pure and Applied Science. Koya University, 2018. http://dx.doi.org/10.14500/icpas2018.mim63.

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Stuecker, Tara, David Newcombe, Myron T. La Duc, Eva Murdock, Randall Sumner e Kasthuri Venkateswaran. "Implications of the VBNC State of B. cepacia and S. maltophilia on Bioreduction and Microbial Monitoring of ISS Potable Waters". In International Conference On Environmental Systems. 400 Commonwealth Drive, Warrendale, PA, United States: SAE International, 2005. http://dx.doi.org/10.4271/2005-01-2933.

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Petersone, Mara, Karlis Ketners e Dainis Krievins. "Integrate health care system performance assessment for value-based health care implementation in Latvia". In Research for Rural Development 2021 : annual 27th International scientific conference proceedings. Latvia University of Life Sciences and Technologies, 2021. http://dx.doi.org/10.22616/rrd.27.2021.018.

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Every year, efforts are applied worldwide, particularly in the European Union, to improve health care systems by increasing the added value of resources already available for health care financing by increasing the performance of health care systems. According to experts of the World Health Organisation (WHO) and the Organisation for Economic Cooperation and Development (OECD), 20–40% of the resources are used for complications that could be avoided, for unnecessary treatment or administrative inefficiency. Therefore, a new initiative to improve health performance – the value-based health care concept (VBHC) is becoming increasingly popular in the world, and particularly in Europe. This scientific article aims to explore the possibilities of applying VBHC in Latvia and the interaction between various management tools in the field of health care. Application of the VBHC concept in Latvia is offered for discussion, where the outcome of the corresponding measure would be identified for each health service provider as part of a one-patient (care) pathway involving several independent health service providers. Based on the Health Care System Performance Assessment (HSPA), clinical (patient) pathways and indicators, to initiate an integrated VBHC model in four priority areas: circulatory system diseases, oncology, mental health, maternal and child health. Meta-analysis of the research is based on the use of qualitative data sources – the existing data sources from policies implemented by the Ministry of Health in Latvia and examples of the introduction of VBHC initiatives worldwide summarised by the VBHC Center Europe. The deductive research is based on the Value-Based Healthcare concept introduced by Porter and Teisberg (2007)
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4

Petersone, Mara, Ingars Erins e Karlis Ketners. "Is Latvia Ready For The Value-Based Healthcare Era?" In 13th International Conference on Applied Human Factors and Ergonomics (AHFE 2022). AHFE International, 2022. http://dx.doi.org/10.54941/ahfe1002130.

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The Value Based Health Care (VBHC) has recently become the leading conceptual approach to health care in the world, but no value-based healthcare programme has yet been established in Latvia. Despite the critical interest in the implementation of VBHC programmes on the part of the health sector stakeholders, still the key persons in VBHC programmes are physicians. Goal of research: To clarify the views of physicians and young physicians on values-based health care fundamental principles and their vision for their further inclusion in Latvian health care. Method: The research uses data from a survey conducted among physicians and young physicians who practice at Pauls Stradins Clinical University Hospital.Results: 42% of physicians responded that patient surveys on the effectiveness of treatment for certain groups of patients could help to improve the results of treatment; 51% of physicians responded that patient surveys on their experience during treatment can help to improve their treatment results; much more cautious were physicians regarding the question whether comparing the results of treatment between physicians teams/hospitals could help to improve the results – 39% replied ‘Yes’ and 24% ‘Rather yes than no’; similarly cautious was the reply to the question whether the voluntary public availability of treatment results from a medical treatment facility could have a positive impact on the visibility of the service provider – 31% replied ‘Yes’ and 25% ‘Rather yes’; the convincing 82% of replies were to the question whether an exchange of experience and knowledge in the team of physicians helps to improve the results of treatment; the convincing majority of 86% of physicians believe that a multidisciplinary team of physicians can help improve the results of treatment; as a positive response, can be considered that 63% of respondents think that reforms in health care may be initiated not only by the Ministry of Health. The most interesting answer would be to the question of what management strategies should be developed in the healthcare system, where the first place, with 349 points, was taken by the answer “Cooperation with external partners (manufacturers, scientific centres, insurers, IT and other service providers)”.Conclusions: The results of the survey show that the application of VBHC principles will not contradict to views of physicians on traditional management models in health care and their role in it.Proposals: However, prior to starting the introduction of VBHC programmes in Latvian health care, there are grounds for launching discussions on the benefits of VBHC compared to the traditional management model. Policy recommendations: For hospital management and health sector supervisors to avoid resistance of physicians to implementing VBHC, the strategy should focus not on VBHC resource-efficient programmes but on patient-centred healthcare.
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Shi, Yuxia, Zhi Jin, Yongbo Su, Yuxiong Cao e Yan Wang. "An improved VBIC model for InP DHBTs". In 2012 IEEE Radio Frequency Integrated Circuits Symposium (RFIC). IEEE, 2012. http://dx.doi.org/10.1109/rfic.2012.6242310.

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Hossain, Md M., W. Alan Davis, Howard T. Russell e Ronald L. Carter. "Thermal characterization of the VBIC dielectrically isolated device". In 2009 IEEE International Conference on Electro/Information Technology (eit '09). IEEE, 2009. http://dx.doi.org/10.1109/eit.2009.5189615.

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7

Jin-Can, Zhang, Zhang Yu-Ming, Lu Hong-Liang, Zhang Yi-Men, Yang Shi e Yuan Peng. "A simplified VBIC model and SDD implementation for InP DHBT". In 2011 International Conference of Electron Devices and Solid-State Circuits (EDSSC). IEEE, 2011. http://dx.doi.org/10.1109/edssc.2011.6117662.

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8

Zhang, Jin-Can, Yu-Ming Zhang, Hong-Liang Lu, Yi-Men Zhang e Hai-Peng Zhang. "The applicability of VBIC and Agilent model on InP DHBTs". In 2012 IEEE 11th International Conference on Solid-State and Integrated Circuit Technology (ICSICT). IEEE, 2012. http://dx.doi.org/10.1109/icsict.2012.6467792.

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9

Aikio, Janne, Joel Vuolevi e Timo Rahkonen. "Detailed Analysis of IMD of HBT PA Based on VBIC Model". In 2006 European Microwave Integrated Circuits Conference. IEEE, 2006. http://dx.doi.org/10.1109/emicc.2006.282678.

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10

Han, Zhijie, Jialiang Lu e Xiaoyu Du. "Topology Design and Algorithm Analysis of VBCC in Hybrid Data Center Network". In WI-IAT '21: IEEE/WIC/ACM International Conference on Web Intelligence. New York, NY, USA: ACM, 2021. http://dx.doi.org/10.1145/3498851.3499001.

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Rapporti di organizzazioni sul tema "VBNC"

1

Bjerg Bennike, Kathrine, Poul Meier Melchiorsen e Anne Lyhne Høj. Forskning og Formidling 2022: Årsrapport VBN. Aalborg University, 2022. http://dx.doi.org/10.54337/aau2022.

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