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Letteratura scientifica selezionata sul tema "Tyrosine – Measurement"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 1 febbraio 2022

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Articoli di riviste sul tema "Tyrosine – Measurement"

1

Li, Yong Jin. "Optical Determination of L-tyrosine Based on Eggshell Membrane Immobilized Tyrosinase". Journal of AOAC INTERNATIONAL 93, n. 6 (1 novembre 2010): 1912–15. http://dx.doi.org/10.1093/jaoac/93.6.1912.

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Abstract An optical biosensor based on the eggshell membrane immobilized tyrosinase is described for the detection of L-tyrosine (L-Tyr). The detection scheme was based on the measurement of absorption value of color adduct resulting from the reaction of 3-methyl-2-benzothiazolinone hydrazone and dopa-quinone produced from the enzymatic oxidation of L-Tyr. The prepared biosensor demonstrated optimum activity at pH 7, optimum temperature range of 2040C and a linear response for the L-Tyr concentration in range of 5200 M. It also showed good operation stability for repeated measurements (over 300 times) and storage stability after it had been kept at 4C for 3 months.

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2

FROST, Matthew T., Barry HALLIWELL e Kevin P. MOORE. "Analysis of free and protein-bound nitrotyrosine in human plasma by a gas chromatography/mass spectrometry method that avoids nitration artifacts". Biochemical Journal 345, n. 3 (25 gennaio 2000): 453–58. http://dx.doi.org/10.1042/bj3450453.

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Measurement of nitrotyrosine in biological fluids and tissues is increasingly being used to monitor the production of reactive nitrogen species in vivo. The detection of nitrotyrosine in vivo has been reported with the use of a variety of methods including immunoassay, HPLC and GLC/MS. The validity of HPLC and immunoassays have been questioned with regard to their selectivity and sensitivity limits. In principle, the measurement of nitrotyrosine by GLC/MS permits a highly specific, highly sensitive and fully quantitative assay. The nitration of tyrosine under acidic conditions in the presence of nitrite is well documented. Derivatization for the full quantification of nitrotyrosine by using GLC/MS can lead to the artifactual nitration of tyrosine if performed under acidic conditions in the presence of nitrite. We describe a novel alkaline method for the hydrolysis and derivatization of nitrotyrosine and tyrosine, and demonstrate its applicability to the measurement of plasma concentrations of both free and protein-bound nitrotyrosine and tyrosine. A detection limit of 1 pg for nitrotyrosine and 100 pg for tyrosine has been achieved. Our method allows, for the first time, the analysis of free and protein-bound nitrotyrosine and tyrosine in biological samples. The plasma concentrations (means±S.E.M.) of free tyrosine and nitrotyrosine in eight normal subjects were 12±0.6 μg/ml and 14±0.7 ng/ml respectively. Plasma proteins contained tyrosine and nitrotyrosine at 60.7±1.7 μg/mg and 2.7±0.4 ng/mg respectively.

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3

Shimizu, H., K. Taniguchi, M. Sugiyama e T. Kanno. "Rapid enzymatic analysis of plasma for tyrosine". Clinical Chemistry 36, n. 1 (1 gennaio 1990): 32–35. http://dx.doi.org/10.1093/clinchem/36.1.32.

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Abstract In this rapid, simple, and convenient enzymatic method for measurement of tyrosine in plasma, tyrosine is converted to tyramine by action of tyrosine decarboxylase (EC 4.1.1.25) and the tyramine produced is oxidized to p-hydroxybenzyl aldehyde and hydrogen peroxide by action of tyramine oxidase (EC 1.4.3.9). The hydrogen peroxide is reacted with 4-aminoantipyrine and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine in the presence of peroxidase (EC 1.11.1.7) to obtain quinoneimine dye, the absorbance of which is measured at 570 nm. Thus tyrosine is measured in the visible range. The CV was 4.6% or less, and the measurement was unaffected by other amino acids, except for phenylalanine. The values obtained (y) correlated well with those obtained with an amino acid analyzer (x): y = 0.902x + 3.92 mumol/L (Syx = 12.3; r = 0.985; n = 54).

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4

Eser, Bekir E., e Paul F. Fitzpatrick. "Measurement of Intrinsic Rate Constants in the Tyrosine Hydroxylase Reaction". Biochemistry 49, n. 3 (26 gennaio 2010): 645–52. http://dx.doi.org/10.1021/bi901874e.

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5

Chung, Inhee. "Optical measurement of receptor tyrosine kinase oligomerization on live cells". Biochimica et Biophysica Acta (BBA) - Biomembranes 1859, n. 9 (settembre 2017): 1436–44. http://dx.doi.org/10.1016/j.bbamem.2017.03.026.

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6

Yang, Yu, Liang-Hong Guo, Na Qu, Ming-Yuan Wei, Li-Xia Zhao e Bin Wan. "Label-free electrochemical measurement of protein tyrosine kinase activity and inhibition based on electro-catalyzed tyrosine signaling". Biosensors and Bioelectronics 28, n. 1 (ottobre 2011): 284–90. http://dx.doi.org/10.1016/j.bios.2011.07.033.

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7

Wei, Lan-Yi, Wei Lin, Bey-Fen Leo, Lik-Voon Kiew, Chia-Ching Chang e Chiun-Jye Yuan. "Development of the Sensing Platform for Protein Tyrosine Kinase Activity". Biosensors 11, n. 7 (15 luglio 2021): 240. http://dx.doi.org/10.3390/bios11070240.

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Abstract (sommario):

A miniature tyrosinase-based electrochemical sensing platform for label-free detection of protein tyrosine kinase activity was developed in this study. The developed miniature sensing platform can detect the substrate peptides for tyrosine kinases, such as c-Src, Hck and Her2, in a low sample volume (1–2 μL). The developed sensing platform exhibited a high reproducibility for repetitive measurement with an RSD (relative standard deviation) of 6.6%. The developed sensing platform can detect the Hck and Her2 in a linear range of 1–200 U/mL with the detection limit of 1 U/mL. The sensing platform was also effective in assessing the specificity and efficacies of the inhibitors for protein tyrosine kinases. This is demonstrated by the detection of significant inhibition of Hck (~88.1%, but not Her2) by the Src inhibitor 1, an inhibitor for Src family kinases, as well as the significant inhibition of Her2 (~91%, but not Hck) by CP-724714 through the platform. These results suggest the potential of the developed miniature sensing platform as an effective tool for detecting different protein tyrosine kinase activity and for accessing the inhibitory effect of various inhibitors to these kinases.

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8

DeFelippis, Michael R., C. P. Murthy, M. Faraggi e Michael H. Klapper. "Pulse radiolytic measurement of redox potentials: the tyrosine and tryptophan radicals". Biochemistry 28, n. 11 (30 maggio 1989): 4847–53. http://dx.doi.org/10.1021/bi00437a049.

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9

Mccaig, T. N., D. Y. K. Fenn, R. E. Knox, R. M. Depauw, J. M. Clarke e J. G. Mcleod. "Measuring polyphenol oxidase activity in a wheat breeding program". Canadian Journal of Plant Science 79, n. 4 (1 ottobre 1999): 507–14. http://dx.doi.org/10.4141/p98-135.

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High levels of polyphenol oxidase (PPO) have been associated with discoloration of end-use products of wheat, especially certain noodle types. Two whole-seed methods of measuring PPO, one based on 10 mM tyrosine as substrate and the other on 90 mM catechol, were examined and modified to determine their potential as screening tools in large-scale breeding programs. Thirteen spring wheat and two spring triticale genotypes were used to compare the methods. Both methods could measure PPO on individual seeds. All genotypes displayed large seed-to-seed variation for PPO with both substrates. The mean coefficient of variation for the PPO values of individual seeds within genotypes was 39% with tyrosine and 34% with catechol. Furthermore, the PPO values of individual seeds within genotypes were not normally distributed for most genotypes. Identifying genotypes with incremental improvements in PPO would probably require measurement of 70–100 seeds. Approximately 50% of the catecholase activity was associated with the water extract after soaking seeds for 16 h, while all of the tyrosinase activity was still associated with the seed, suggesting that different enzymes are responsible for oxidizing tyrosine and catechol in wheat. While the 10 mM tyrosine assay was nondestructive and allowed plants to be generated from seeds low in PPO, 90 mM catechol reduced germination to less than 20%. Reducing the catechol to 30 mM improved germination to 85%, did not substrate-limit the reaction, and reduced the health risk associated with the assay. Spectral and kinetic differences between the assays were also considered. Key words: Triticum sp., wheat, polyphenol oxidase, catechol, tyrosine

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10

Kobayashi, Tomoko, Shun-Ichi Nakamura e Hirohei Yamamura. "Cytosolic Protein-Tyrosine Kinase Activities in Various Rat Tissues". Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 26, n. 2 (marzo 1989): 164–68. http://dx.doi.org/10.1177/000456328902600213.

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Suitable assay conditions for the detection of cytosolic protein-tyrosine kinase activities in crude extracts of various rat tissues have been determined. Cytosolic protein-tyrosine kinases showed common characteristics including substrate specificity and divalent cation requirement. Using (Val5) angiotensin II and Mn2+ rather than a src-related synthetic peptide, E11G1, and Mg2+, we obtained higher activities of cytosolic protein-tyrosine kinases. Among various rat tissues tested, spleen, bone marrow, thymus, small intestine, appendix and lung, in decreasing order of total activity, contained high activities of cytosolic protein-tyrosine kinases. These results suggest that the enzyme activities in lymphatic organs and in organs closely related to cell proliferation are high. The assay system described allows the precise measurement of cytosolic protein-tyrosine kinase activity in various rat tissues, both normal and malignant.

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Più fonti

Tesi sul tema "Tyrosine – Measurement"

1

Thorpe, Jane Marie. "The effect of tyrosine and prior protein intake on the measurement of phenylalanine metabolism in vivo". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape9/PQDD_0006/NQ41326.pdf.

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2

Bucknall, Martin Paul Medical Sciences Faculty of Medicine UNSW. "Dityrosine as a biomarker of free radical induced oxidative damage in diseases of ageing". Awarded by:University of New South Wales. School of Medical Sciences, 2006. http://handle.unsw.edu.au/1959.4/30207.

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o,o???-Dityrosine (dityrosine), an oxidation product of tyrosine produced by reaction between tyrosyl radicals, is becoming established as a biomarker of free radical oxidative protein damage in vivo. Attempts to measure dityrosine concentrations in various physiological and pathological systems have produced varied and often contradictory results. Dityrosine concentrations in urine, plasma, cerebrospinal fluid (CSF) and brain tissue varying over three orders of magnitude have been reported, together with inconsistent claims of significant dityrosine elevation in several ageing-related pathologies. Some of these findings have contributed to the implication of free radical activity in the pathology of several neurodegenerative disorders, vascular and ocular abnormalities and in phagocyte response to infection. The aim of this study was to test the hypothesis that dityrosine levels are elevated in ageing and ageing-related disease. The study also aims to determine the utility of dityrosine measurement as an index of oxidative damage, and elucidate possible explanations for the inconsistent levels reported. An assay for the quantification of dityrosine was developed using capillary HPLC with electrospray tandem quadrupole mass spectrometry (HPLC-MS/MS). The assay was highly specific for dityrosine and has the highest absolute sensitivity for dityrosine of any method reported to date, with a detection limit of 3 femtomoles of dityrosine on-column. Urine samples from volunteers of different age and from hospital patients with various pathologies were analysed. Plasma protein hydrolysates from control, Alzheimer???s and stroke subjects were analysed, together with hydrolysates of post mortem brain tissue from Alzheimer???s and control subjects. Urinary dityrosine level is elevated in states of acute infection and inflammation, but does not correlate with age or chronic disease. Protein dityrosine in four sections of Alzheimer???s brain was not significantly different from control sections. Dityrosine was present in human plasma and tissue proteins at approximately 5-35 residues per million tyrosine residues, and in normal urine at 5-25 micromol/mol creatinine or 20-200 nM. Most of the discrepancies in the literature relate to inadequate specificity of the analytical method. Interpretation of published data with critical appraisal of measurement technology specificity is essential in developing an accurate understanding of the role of free radicals in ageing and disease.

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3

Streffer, Katrin. "Highly sensitive measurements of substrates and inhibitors on the basis of tyrosinase sensors and recycling systems". Phd thesis, [S.l. : s.n.], 2002. http://pub.ub.uni-potsdam.de/2003/0001/streffer.pdf.

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4

Streffer, Katrin [Verfasser]. "Highly sensitive measurements of substrates and inhibitors on the basis of tyrosinase sensors and recycling systems / von Katrin Streffer". 2002. http://d-nb.info/967537118/34.

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5

Gajula, M. N. V. Prasad. "Computer simulation meets experiment: Molecular dynamics simulaitons of spin labeled proteins". Doctoral thesis, 2008. https://repositorium.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2008041631.

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Abstract (sommario):

EPR spectroscopy of site-directed spin labeled proteins is extremely informative in the studies of protein dynamics; however, it is difficult to interpret the spectra in terms of the conformational dynamics in atomic detail.In the present work we aimed to investigate the site-specific structural dynamics of proteins by using MD simulations upon analyzing and interpreting the EPR data. The major goal of this work is to know how far the computer simulations can meet the experiments. As a first step, MD simulations are performed to identify the location and orientation of the tyrosine radical in the R2 subunit of ribonucleotide reductase. The MD results show that the tyrosine is moving away from the diiron center in its radical state. This data is in agreement with EPR results and suggests reorientation of the tyrosine radical when compared to its neutral state. In further studies, the behavior of a methanethiosulfonate spin label, R1, in various environments of the protein is characterized by using MD simulations. RMSD analysis and angle ß distributions of the nitroxide show that R1 in buried sites in a protein helix is significantly immobile and in surface exposed sites it is highly mobile. Analyses of MD data suggest that internal rotations of x4 and x5 dihedrals of R1 are dominant in the R1 dynamics.Our studies also show that interaction with the surrounding residues show significant influence on the dynamics of R1. MD simulations data of the vinculin tail protein, both in water and in vacuo, are compared to the experimental results for further analysis of 12 different R1 sites in various environments.In a study on the photosynthetic reaction center(RC),MD is used to identify the location of the R1 binding site (H156)and thereby exploring the conformational dynamics in the RC protein upon light activation. The distance between the primary quinone, QA, and H156R1 determined from MD is in reasonable agreement with that measured by EPR.

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Libri sul tema "Tyrosine – Measurement"

1

Thorpe, Jane Marie. The effect of tyrosine and prior protein intake on the measurement of phenylalanine metabolism in vivo. 1999.

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Capitoli di libri sul tema "Tyrosine – Measurement"

1

Robins, Eli. "The Measurement of Phenylalanine and Tyrosine in Blood". In Methods of Biochemical Analysis, 287–309. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2006. http://dx.doi.org/10.1002/9780470110355.ch6.

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2

Eigner, Sebastian, Denis R. Beckford Vera, Marco Fellner, Natalia S. Loktionova, Markus Piel, Frantisek Melichar, Frank Rösch, Tobias L. Roß, Ondrej Lebeda e Katerina Eigner Henke. "Measurement of Protein Synthesis: In Vitro Comparison of 68Ga-DOTA-Puromycin, [3H]Tyrosine, and 2-Fluoro-[3H]tyrosine". In Recent Results in Cancer Research, 269–83. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-27994-2_14.

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3

Waisbren, Susan E., Sanjay P. Prabhu, Patricia Greenstein, Carter Petty, Donald Schomer, Vera Anastasoaie, Kalin Charette, Daniel Rodriguez, Sai Merugumala e Alexander P. Lin. "Improved Measurement of Brain Phenylalanine and Tyrosine Related to Neuropsychological Functioning in Phenylketonuria". In JIMD Reports, 77–86. Berlin, Heidelberg: Springer Berlin Heidelberg, 2016. http://dx.doi.org/10.1007/8904_2016_11.

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4

Tsikas, Dimitrios, Anja Mitschke e Frank-Mathias Gutzki. "Measurement of 3-Nitro-Tyrosine in Human Plasma and Urine by Gas Chromatography-Tandem Mass Spectrometry". In Methods in Molecular Biology, 255–70. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-445-2_20.

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5

"Cancers of the genitourinary system". In Oxford Desk Reference: Oncology, a cura di Thankamma Ajithkumar, Ann Barrett, Helen Hatcher e Sarah Jefferies, 196–233. Oxford University Press, 2021. http://dx.doi.org/10.1093/med/9780198745440.003.0008.

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This chapter deals with renal, bladder, prostate and penile cancers, and tumours of the testis. Epidemiology, aetiology, and risk factors including specific genetic mutations as well as general lifestyle factors are described for each tumour type. For renal tumours, classification takes into account different clinical behaviours and genetic mutations. The role of surgery in the cure of disease and treatment of metastatic disease are discussed as well as the role of radiotherapy, chemotherapy, and the use of tyrosine kinase, mTor, and T-cell checkpoint inhibition. The role of surgery in bladder cancer is defined in the management of localized and muscle invasive cancer and the use of chemotherapy, radiotherapy, or both in advanced or metastatic disease. As well as discussion of the different aspects of management of prostate cancer, consideration is given to the use of absolute values of PSA measurement and other parameters in screening, treatment monitoring, and surveillance. Endocrine therapies are also discussed. Epidemiology aetiology, genetic factors, and pathology of testicular tumours are considered and the curative potential of treatment is underlined as well as the appropriate use of surveillance. For penile cancer treatment, modalities including surgery and different radiotherapy approaches are outlined.

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Eisen, Tim, Freddie C. Hamdy e Robert A. Huddart. "Malignant diseases of the urinary tract". In Oxford Textbook of Medicine, a cura di John D. Firth, 5136–49. Oxford University Press, 2020. http://dx.doi.org/10.1093/med/9780198746690.003.0508.

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Bladder cancer—the seventh commonest cancer in the United Kingdom and the fourth most common in men. Nonmuscle-invasive disease is usually treated by transurethral resection with postoperative intravesical chemotherapy with mitomycin or bacillus Calmette–Guérin. Local muscle-invasive disease in patients who are fit enough is usually treated with radical cystoprostatectomy and cisplatin-based chemotherapy. Metastatic disease is typically treated with cisplatin-based chemotherapy. Renal cell cancer—approximately 3% of the total cancer burden. For operable patients with no distant disease, the treatment of choice is nephron-sparing (if possible) or radical nephrectomy. Metastatic renal cancer can behave in a very variable manner. Palliative nephrectomy may be required for bleeding or pain. First-line systemic treatment is with antiangiogenic tyrosine kinase inhibitors targeting vascular endothelial growth factor receptor signalling. Prostate cancer—second most common cause of male cancer deaths in the Western world. Most cases are asymptomatic at presentation, being detected following measurement of serum prostate-specific antigen (PSA) or after digital rectal examination, although screening by measurement of PSA remains a contentious issue. Clinically localized prostate cancer is treated with active monitoring, radiotherapy, or minimally invasive surgery. Locally advanced disease is likely to progress and requires intervention, usually in the form of androgen deprivation therapy and radiotherapy. First-line treatment for metastatic prostate cancer is androgen deprivation therapy; second-line treatment may be with newer antiandrogens in combination with steroids and cytotoxics. Testicular cancer—affects predominantly young adult men in whom they are the most common malignant tumours. For most patients, initial management consists of an inguinal orchidectomy, with or without immediate adjuvant therapy. Standard treatment of metastatic germ cell tumours is with a combination of bleomycin, etoposide, and cisplatin.

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Sweeney, Mark, e Alexander Lyon. "Cardiovascular complications of novel kinase inhibitors". In ESC CardioMed, 1170–76. Oxford University Press, 2018. http://dx.doi.org/10.1093/med/9780198784906.003.0292.

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Tyrosine and serine kinase inhibitors are new cancer therapies which have revolutionized the field of modern oncology and have transformed previously fatal conditions such as chronic myeloid leukaemia into treatable chronic diseases. The cardiovascular complications related to kinase inhibitor treatments have emerged and the importance of effective cardiovascular management is becoming clear. Precise cardiovascular toxicity profiles vary among different classes of kinase inhibitors and between different specific agents. The most important cardiovascular complications of these kinase inhibitors include hypertension, heart failure, arterial thromboembolic disease, QT prolongation, and pulmonary hypertension. The aim of effective cardiovascular management in this setting should be to minimize the impact of toxicities to facilitate the continuation of these highly effective cancer treatments. It is key to perform a thorough baseline cardiovascular risk assessment prior to commencing treatment so that reversible risk factors are identified and addressed appropriately. Preliminary assessment should, at a minimum, include blood pressure measurement, serum creatinine, lipid profile and fasting glucose, and a resting electrocardiogram. Additionally, in selected high-risk patients or those starting high-risk kinase inhibitors, baseline assessment of left ventricular function can also be helpful. Effective risk stratification will allow appropriate, individualized surveillance to be arranged for each patient in order to identify complications early and manage them effectively. When significant cardiovascular complications occur, decisions regarding the need to suspend or discontinue potentially life-prolonging treatments are exceptionally challenging and the input of the multidisciplinary team in this setting and communication between the cardiologists and the oncologists or haemato-oncologists is invaluable.

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Atti di convegni sul tema "Tyrosine – Measurement"

1

Habiboglu, M. Gokhan, e Sahin Uyaver. "Shape measurement for cylindirical structures formed by tyrosine molecules". In THIRD INTERNATIONAL CONFERENCE OF MATHEMATICAL SCIENCES (ICMS 2019). AIP Publishing, 2019. http://dx.doi.org/10.1063/1.5136196.

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2

Phillips, Ryan M., David S. Lawrence, Christopher E. Sims e Nancy L. Allbritton. "Abstract 4128: A novel approach to the measurement of tyrosine phosphorylation dynamics in intact single cells using capillary electrophoresis." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-4128.

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3

Labots, Mariette, Kristy J. Gotink, Henk Dekker, Johannes C. van der Mijn, Henk J. Broxterman, Maria N. Rovithi, Connie R. Jiménez e Henk M. W. Verheul. "Abstract 3608: Measurement of kinase activity in cancer cell lines and tumor tissue using a tyrosine kinase peptide substrate array". In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-3608.

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4

Gong, Shengzhao, Qingsheng Chen e Mengyi Xu. "Inhibitory Kinetics of 2'-Hydroxy-4'-methoxyacetophenone on Tyrosinase-catalyzing Reaction". In 2015 4th International Conference on Sensors, Measurement and Intelligent Materials. Paris, France: Atlantis Press, 2016. http://dx.doi.org/10.2991/icsmim-15.2016.117.

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