Bibliografie tematiche / Substrate identification

Letteratura scientifica selezionata sul tema "Substrate identification"

Autore: Grafiati

Pubblicato: 8 giugno 2024

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Articoli di riviste sul tema "Substrate identification":

Malintoi, Adrianus, Inneke F. M. Rumengan, Kakaskasen A. Roeroe, Veibe Warouw, Ari B. Rondonuwu e Medy Ompi. "KOMUNITAS ASCIDIA DI PESISIR MALALAYANG DUA, TELUK MANADO, SULAWESI UTARA". JURNAL PESISIR DAN LAUT TROPIS 8, n. 1 (15 gennaio 2020): 39. http://dx.doi.org/10.35800/jplt.8.1.2020.27403.

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Field survey on ascidian community was conducted along the coastal area of Malalayang Dua in order to find out species of ascidia, species abundance, and ascidian substrates. A survey method and quadrant transects were applied. Pictures were taken, while species and their substrates were sampled. Species identification was based on morphological characteristics, while substrate type identification was based on ascidian species attachment. The results shows that 21 ascidian species were found in the the coastal of Malalayang Dua. Didemnum molle was the highest abundant species in the area, followed by Polycarpa aurata, Polycarpa sp.4. and Polycarpa sp.2.. Dead coral algaes (DCA) were found to be the most preferred substrates by ascidians in the area. Keywords : ascidia, species, substrate, distribution, and abundance Survei lapangan terhadap komunitas ascidia dilakukan di pesisir Malalayang Dua untuk mendapatkan data jenis, kelimpahan, dan substrat ascidia. Metode yang digunakan yaitu metode survei jelajah dan transek kuadran. Identifikasi jenis ascidia dilakukan berdasarkan karakteristik morfologi. Hasil penelitian ditemukan ada 21 jenis ascidia. Substrat jenis death coral algae (DCA) merupakan substrat yang paling banyak ditempati ascidia. Kelimpahan ascidia tertinggi adalah Didemnum molle di pesisir Malalayang Dua, diikuti oleh Polycarpa aurata, Polycarpa sp.4. dan Polycarpa sp.2. Death coral alga (DCA) ditemukan sebagai substrat yang paling disukai oleh ascidia di daerah itu. Kata Kunci : ascidia, spesies, substrat, distribusi, dan kelimpahan

Baros, Seanantha S., Jonathan M. Blackburn e Nelson C. Soares. "Phosphoproteomic Approaches to Discover Novel Substrates of Mycobacterial Ser/Thr Protein Kinases". Molecular & Cellular Proteomics 19, n. 2 (15 dicembre 2019): 233–44. http://dx.doi.org/10.1074/mcp.r119.001668.

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Mycobacterial Ser/Thr protein kinases (STPKs) play a critical role in signal transduction pathways that ultimately determine mycobacterial growth and metabolic adaptation. Identification of key physiological substrates of these protein kinases is, therefore, crucial to better understand how Ser/Thr phosphorylation contributes to mycobacterial environmental adaptation, including response to stress, cell division, and host-pathogen interactions. Various substrate detection methods have been employed with limited success, with direct targets of STPKs remaining elusive. Recently developed mass spectrometry (MS)-based phosphoproteomic approaches have expanded the list of potential STPK substrate identifications, yet further investigation is required to define the most functionally significant phosphosites and their physiological importance. Prior to the application of MS workflows, for instance, GarA was the only known and validated physiological substrate for protein kinase G (PknG) from pathogenic mycobacteria. A subsequent list of at least 28 candidate PknG substrates has since been reported with the use of MS-based analyses. Herein, we integrate and critically review MS-generated datasets available on novel STPK substrates and report new functional and subcellular localization enrichment analyses on novel candidate protein kinase A (PknA), protein kinase B (PknB) and PknG substrates to deduce the possible physiological roles of these kinases. In addition, we assess substrate specificity patterns across different mycobacterial STPKs by analyzing reported sets of phosphopeptides, in order to determine whether novel motifs or consensus regions exist for mycobacterial Ser/Thr phosphorylation sites. This review focuses on MS-based techniques employed for STPK substrate identification in mycobacteria, while highlighting the advantages and challenges of the various applications.

Peerce, B. E. "Identification of the intestinal Na-phosphate cotransporter". American Journal of Physiology-Gastrointestinal and Liver Physiology 256, n. 4 (1 aprile 1989): G645—G652. http://dx.doi.org/10.1152/ajpgi.1989.256.4.g645.

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Na-dependent phosphate uptake in intestinal brush-border membrane vesicles was sensitive to arginine group-specific reagents in a substrate-sensitive manner. Four different arginine group-specific reagents were tested. All four reagents irreversibly inhibited Na-dependent phosphate uptake with the concentration for 50% inhibition, K0.5, varying between 150 and 40 microM. Maximum inhibition approached 80%. Addition of substrates during exposure to these reagents resulted in protection of Na-phosphate cotransport only in the presence of Na and phosphate. Na-phosphate cotransporter labeling at or near the phosphate site was accomplished using a pretreatment step with phenylglyoxal and substrates followed by a fluorescent phenylglyoxal-labeling step. Fluorescein isothiocyanate-phenylglyoxal (FITC-PG) specifically labeled a 130-kDa polypeptide on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in a substrate-sensitive manner, consistent with its effect on Na-phosphate cotransport. n-Acetylimidazole (NAI) inhibited Na-phosphate cotransport in a Na+- but not K+ -sensitive manner. NAI or fluorescein n-acetylimidazole (FNAI) inhibited Na-dependent phosphate uptake with a K0.5 for inhibition of 38 microM. Maximum inhibition of Na-phosphate cotransport was 75%. On SDS-PAGE, FNAI labeled five polypeptide bands in a Na-sensitive manner including the 130-kDa polypeptide band labeled by FITC-PG. Of these five bands only the 130-kDa polypeptide lost substrate protectability against FITC-PG inhibition of Na-phosphate cotransport and FITC-PG labeling on prior exposure to NAI in the absence of Na+. On this basis the 130-kDa polypeptide is tentatively identified as the intestinal Na-phosphate cotransporter and this polypeptide band contains both the Na substrate site and the phosphate substrate site.

Gopalakrishnan, Ramakrishnan, Sivakumar Rajagopal, Sai Viswanth Reddy e Anirudh E. R. "Identification of Most Suitable Dielectrics Substrate for UWB Bandpass Filter". ECS Transactions 107, n. 1 (24 aprile 2022): 431–38. http://dx.doi.org/10.1149/10701.0431ecst.

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Identification of a suitable substrate is a critical parameter in the design of microstrip bandpass filters. In this work, the performance of an Ultra Wideband (UWB) bandpass filter based on Electromagnetic Bandgap (EBG) resonators are designed, fabricated, and analyzed. Printed Circuit Board (PCB) with suitable dielectric substrates are used extensively to develop microstrip lines. The filter is fabricated using RT Duroid substrate material and compared with FR4 and Alumina dielectric substrates and the performance results show that the best result is obtained with RT Duroid.

SONG, JIANGNING, HAO TAN, SARAH E. BOYD, HONGBIN SHEN, KHALID MAHMOOD, GEOFFREY I. WEBB, TATSUYA AKUTSU, JAMES C. WHISSTOCK e ROBERT N. PIKE. "BIOINFORMATIC APPROACHES FOR PREDICTING SUBSTRATES OF PROTEASES". Journal of Bioinformatics and Computational Biology 09, n. 01 (febbraio 2011): 149–78. http://dx.doi.org/10.1142/s0219720011005288.

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Proteases have central roles in "life and death" processes due to their important ability to catalytically hydrolyze protein substrates, usually altering the function and/or activity of the target in the process. Knowledge of the substrate specificity of a protease should, in theory, dramatically improve the ability to predict target protein substrates. However, experimental identification and characterization of protease substrates is often difficult and time-consuming. Thus solving the "substrate identification" problem is fundamental to both understanding protease biology and the development of therapeutics that target specific protease-regulated pathways. In this context, bioinformatic prediction of protease substrates may provide useful and experimentally testable information about novel potential cleavage sites in candidate substrates. In this article, we provide an overview of recent advances in developing bioinformatic approaches for predicting protease substrate cleavage sites and identifying novel putative substrates. We discuss the advantages and drawbacks of the current methods and detail how more accurate models can be built by deriving multiple sequence and structural features of substrates. We also provide some suggestions about how future studies might further improve the accuracy of protease substrate specificity prediction.

Dauksher, Walter, Scott Burton, David Niles e Dennis H. Eaton. "Identification of Poor Via-Ceramic Adhesion in Electronic Substrates". EDFA Technical Articles 4, n. 1 (1 febbraio 2002): 5–10. http://dx.doi.org/10.31399/asm.edfa.2002-1.p005.

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Abstract Mechanical bending strength measurements, part of a quality screening process for ceramic substrate package designs, revealed a significant difference between two substrates that could not be attributed to constituent materials or structural thickness effects. Structural analysis was performed to generate a hypothesis that the metal vias were not structurally adhered to the ceramic holes and thus caused stress concentrations that reduced the bending strength of the substrates. In this detailed case study, the authors explain how they proved their hypothesis using FEA to calculate stress concentration factors, optical microscopy to examine fracture surfaces, and electron spectroscopy to determine chemical compositions. The successful outcome attests to the value of strength criteria as an investigative tool for the assessment of electronic package substrate quality.

Mizunuma, Masataka, Atsushi Kaneko, Shunta Imai, Kazuhiro Furukawa e Yoshiro Chuman. "Methods for Identification of Substrates/Inhibitors of FCP/SCP Type Protein Ser/Thr Phosphatases". Processes 8, n. 12 (4 dicembre 2020): 1598. http://dx.doi.org/10.3390/pr8121598.

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Protein phosphorylation is the most widespread type of post-translational modification and is properly controlled by protein kinases and phosphatases. Regarding the phosphorylation of serine (Ser) and threonine (Thr) residues, relatively few protein Ser/Thr phosphatases control the specific dephosphorylation of numerous substrates, in contrast with Ser/Thr kinases. Recently, protein Ser/Thr phosphatases were reported to have rigid substrate recognition and exert various biological functions. Therefore, identification of targeted proteins by individual protein Ser/Thr phosphatases is crucial to clarify their own biological functions. However, to date, information on the development of methods for identification of the substrates of protein Ser/Thr phosphatases remains scarce. In turn, substrate-trapping mutants are powerful tools to search the individual substrates of protein tyrosine (Tyr) phosphatases. This review focuses on the development of novel methods for the identification of Ser/Thr phosphatases, especially small C-terminal domain phosphatase 1 (Scp1), using peptide-displayed phage library with AlF4−/BeF3−, and discusses the identification of putative inhibitors.

Ye, Siying, Siew Yeen Chai, Rebecca A. Lew, David B. Ascher, Craig J. Morton, Michael W. Parker e Anthony L. Albiston. "Identification of modulating residues defining the catalytic cleft of insulin-regulated aminopeptidase". Biochemistry and Cell Biology 86, n. 3 (aprile 2008): 251–61. http://dx.doi.org/10.1139/o08-037.

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Inhibition of insulin-regulated aminopeptidase (IRAP) has been demonstrated to facilitate memory in rodents, making IRAP a potential target for the development of cognitive enhancing therapies. In this study, we generated a 3-D model of the catalytic domain of IRAP based on the crystal structure of leukotriene A4 hydrolase (LTA4H). This model identified two key residues at the ‘entrance’ of the catalytic cleft of IRAP, Ala427 and Leu483, which present a more open arrangement of the S1 subsite compared with LTA4H. These residues may define the size and 3-D structure of the catalytic pocket, thereby conferring substrate and inhibitor specificity. Alteration of the S1 subsite by the mutation A427Y in IRAP markedly increased the rate of substrate cleavage V of the enzyme for a synthetic substrate, although a corresponding increase in the rate of cleavage of peptide substrates Leu-enkephalin and vasopressin was was not apparent. In contrast, [L483F]IRAP demonstrated a 30-fold decrease in activity due to changes in both substrate affinity and rate of substrate cleavage. [L483F]IRAP, although capable of efficiently cleaving the N-terminal cysteine from vasopressin, was unable to cleave the tyrosine residue from either Leu-enkephalin or Cyt6-desCys1-vasopressin (2–9), both substrates of IRAP. An 11-fold reduction in the affinity of the peptide inhibitor norleucine1-angiotensin IV was observed, whereas the affinity of angiotensin IV remained unaltered. In additionm we predict that the peptide inhibitors bind to the catalytic site, with the NH2-terminal P1 residue occupying the catalytic cleft (S1 subsite) in a manner similar to that proposed for peptide substrates.

Zhao, Yi, Eliud Morales Morales Dávila, Xue Li, Beiyu Tang, Ariana I. Rabinowitsch, Jose Manuel Perez-Aguilar e Carl P. Blobel. "Identification of Molecular Determinants in iRhoms1 and 2 That Contribute to the Substrate Selectivity of Stimulated ADAM17". International Journal of Molecular Sciences 23, n. 21 (24 ottobre 2022): 12796. http://dx.doi.org/10.3390/ijms232112796.

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The metalloprotease ADAM17 is a key regulator of the TNFα, IL-6R and EGFR signaling pathways. The maturation and function of ADAM17 is controlled by the seven-membrane-spanning proteins iRhoms1 and 2. The functional properties of the ADAM17/iRhom1 and ADAM17/iRhom2 complexes differ, in that stimulated shedding of most ADAM17 substrates tested to date can be supported by iRhom2, whereas iRhom1 can only support stimulated shedding of very few ADAM17 substrates, such as TGFα. The first transmembrane domain (TMD1) of iRhom2 and the sole TMD of ADAM17 are important for the stimulated shedding of ADAM17 substrates by iRhom2. However, little is currently known about how the iRhoms interact with different substrates to control their stimulated shedding by ADAM17. To provide new insights into this topic, we tested how various chimeras between iRhom1 and iRhom2 affect the stimulated processing of the EGFR-ligands TGFα (iRhom1- or 2-dependent) and EREG (iRhom2-selective) by ADAM17. This uncovered an important role for the TMD7 of the iRhoms in determining their substrate selectivity. Computational methods utilized to characterize the iRhom1/2/substrate interactions suggest that the substrate selectivity is determined, at least in part, by a distinct accessibility of the substrate cleavage site to stimulated ADAM17. These studies not only provide new insights into why the substrate selectivity of stimulated iRhom2/ADAM17 differs from that of iRhom1/ADAM17, but also suggest new approaches for targeting the release of specific ADAM17 substrates.

Zhai, Jingyu, Yugang Chen, Xinyuan Song, Hongchun Wu e Qingkai Han. "Identification of the Anisotropic Elastic Parameters of NiCrAlY Coating by Combining Nanoindentation and Finite Element Method". Shock and Vibration 2019 (2 giugno 2019): 1–13. http://dx.doi.org/10.1155/2019/9034750.

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For vibration damping, coatings are prepared on surface of the structures (substrates), which constitute the coating-substrate composite structures. Elastic parameters of the coating are indispensable for the vibration and damping analysis of the composite structure. Due to the small scale of coating thickness and elastic difference compared with the substrate, the identification results are inevitably influenced by the existence of substrate. Moreover, resulting from the preparation process, elastic properties of hard coating often exhibit anisotropic properties. All the above factors bring about the difficulties of accurate identification. In this study, a method for identifying anisotropic elastic parameters of hard coatings considering substrate effect is proposed, by combining nanoindentation and finite element analysis. Based on the identification results, finite element models are established to analyze the vibration characteristics of the coating-substrate composite structure, which verify the rationality of the anisotropic elastic parameters for vibration analysis. The studies in this paper are significant to more accurately identify the mechanical parameters for establishing the dynamic model. Moreover, they lay the foundation for further optimization design of hard coating damping.

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Tesi sul tema "Substrate identification":

Thomas, Daniel Alexander. "Application of peptide and cDNA libraries to protease substrate identification". Thesis, University of Leeds, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.418926.

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Redbird, Ruth Ann. "Identification of a protein kinase substrate in Sulfolobus solfataricus P2". Diss., Virginia Tech, 2010. http://hdl.handle.net/10919/26884.

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Living organisms rely on many different mechanisms to adapt to changes within their environment. Protein phosphorylation and dephosphorylation events are one such way cells can communicate to generate a response to environmental changes. In the Kennelly laboratory we hope to gain insight on phosphorylation events in the domain Archaea through the study of the acidothermophilic organism Sulfolobus solfataricus. Such findings may provide answers into evolutionary relationships and facilitate an understanding of phosphate transfer via proteins in more elaborate systems where pathway disturbances can lead to disease processes. A λ-phage expression library was generated from S. solfataricus genomic DNA. The immobilized expression products were probed with a purified protein kinase, SsoPK4, and radiolabeled ATP to identify potential native substrates. A protein fragment of the ORF sso0563, the catalytic A-type ATPase subunit A (AtpA), was phosphorylated by SsoPK4. Full length and truncated forms of AtpA were overexpressed in E. coli. Additional subunits of the ATPase were also overexpressed and ATPase activity reconstituted in vitro. Phosphoamino acid analysis and MS identified the phosphorylation sites on AtpA. Several variants of AtpA were derived via site-directed mutagenesis and assayed for ATPase activity. Chemical cross-linking was employed to determine possible ATPase subunit interactions; tryptic digests of AtpA and its mutant variants were performed to examine protein folding. The phosphorylated-mimic variant of AtpA, T98D, resulted in an inactive ATPase complex as determined by ATPase activity assays and native-PAGE indicating potential phosphoregulation by SsoPK4 on enzyme activity. Ultimately, any findings would need verification with in vivo studies.
Ph. D.

Zhang, Peng. "Functional Characterization of Protein Tyrosine Phosphatases in Tumorigenesis through Substrate Identification". Case Western Reserve University School of Graduate Studies / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=case1365174835.

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Campbell, Timothy. "Methods for Arrhythmogenic Substrate Identification and Procedural Improvements for Ventricular Arrhythmias". Thesis, The University of Sydney, 2022. https://hdl.handle.net/2123/29925.

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Ventricular arrhythmias (VA) are a frequent precursor to sudden cardiac death (SCD) in patients with structural heart disease (SHD). Patients with SHD are at risk of recurrent ventricular tachycardia (VT), which generally occurs due to re-entry within and around the presence of an arrhythmogenic scar. Therefore, scarred myocardium forms the necessary substrate for arrhythmogenesis to occur. A scar may occur due to obstructive coronary artery disease, causing ischaemic cardiomyopathy (ICM), or from cardiac injury due to several other causes, including inflammatory, infiltrative, toxin-mediated, or genetic heart disease, termed non-ischaemic cardiomyopathy (NICM). An implantable cardioverting defibrillator (ICD) can abort SCD from recurrent VAs. However, they do not stop VAs from occurring in the first place. Anti-arrhythmic drugs (AADs) may reduce the frequency and burden of VAs but have limited efficacy. Some have a narrow therapeutic window or the potential for multiorgan toxicity and can be poorly tolerated. Catheter ablation (CA) is a class I indication for treating sustained monomorphic VT refractory to AADs. CA reduces VT burden, the number of defibrillator therapies, greater freedom from recurrent ventricular arrhythmia, and improves quality of life. However, recurrences can be experienced in up to 50% of patients with SHD-related VT. Some reasons for the failure of CA include reliable identification of critical components of substrate that can harbour VAs both in sinus rhythm and during ongoing VT using electroanatomic mapping (EAM) and imaging techniques, as well as limitations in assessing intraprocedural endpoints. Further refinement of electroanatomic mapping techniques is required to improve the efficacy of CA. This thesis aims to expand on current techniques for substrate identification and methods to improve the efficacy of VA ablation procedures.

Chin, Wing Hong. "Identification of TrkB as a p35 interacting protein and a Cdk5 substrate /". View abstract or full-text, 2005. http://library.ust.hk/cgi/db/thesis.pl?BICH%202005%20CHIN.

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Humphreys, D. "Identification of a novel substrate of the Salmonella protein tyrosine phosphatase SptP". Thesis, University of Cambridge, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.604780.

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A novel cellular target was captured when purified SptP substrate-trapping derivatives and cell extracts were combined, which was isolated and identified by MALDI-TOF mass spectrometry as VCP (valosin-containing protein). VCP is a AAA family ATPase that regulates multiple ubiquitin-dependent cellular processes, including proteasome-dependant protein degradation, protein-membrane association and organelle biogenesis. Next, interaction between native SptP and VCP was reconstituted in vitro. Further biochemical analyses revealed that binding was blocked by phosphatase inhibitors and required both SptP domains (GAP and PTP). Furthermore, SptP exhibited potent phosphatase activity towards VCP in vitro, and TTSS-delivered SptP associated with and specifically dephosphorylated VCP during infection of cultured cells with S. typhimurium. Two additional entry effectors, the Salmonella GTP exchange factor mimics SopE/2 and inositol phosphatase SopB are ubiquitinated following their delivery into target cells. Immunoprecipitation of SopE2 and SopB from cells following infection with a S. typhimurium ΔsptP null mutant localisation occurred independently of SptP and therefore VCP dephosphorylation. Bacterial entry efficiency was unaffected by VCP gene silencing by RNA interference (RNAi), suggesting that SptP-dependent VCP dephosphorylation was relevant later during infection. Such a role was further supported by immunofluorescence and immunoprecipitation analyses demonstrating that SptP persists within infected cells. Immunofluorescence microscopy showed that both SptP and VCP transiently associate with internalised S. typhimurium and that this SptP targeting was dependent upon it s GAP activity. Infection with S. typhimurium engineered to delivery augmented levels of SptP resulted in a 2.1-fold increase in bacterial replication 8-hours post-infection and reciprocally, a 1.7-fold reduction was observed at equivalent time-points after cells were infected with the S. typhimurium ΔsptP mutant. VCP knockdown resulted in increased bacterial replication and parallel immunofluorescence microscopy revealed elevated numbers of Salmonella localised in the infected cell cytosol. Examination of wild-type SCV morphology after VCP knockdown demonstrated that Sif formation was abolished. This thesis reports that the Salmonella entry effector SptP persists after invasion and regulates S. typhimurium replication at late stages of infection through alteration of the phosphorylation status of VCP, a novel cellular target of the SptP PTP domain located on the SCV.

Kusevic, Denis [Verfasser], e Albert [Akademischer Betreuer] Jeltsch. "Biochemical investigation of the substrate specificity of protein methyltransferases and the identification of novel substrates / Denis Kusevic ; Betreuer: Albert Jeltsch". Stuttgart : Universitätsbibliothek der Universität Stuttgart, 2016. http://d-nb.info/1165574489/34.

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Boswell, Nicholas William Bradford. "Biochemical characterization of the [FeFe]-hydrogenase maturation protein HydE and identification of the substrate". Thesis, Montana State University, 2011. http://etd.lib.montana.edu/etd/2011/boswell/BoswellN1211.pdf.

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Hydrogenases catalyze the reversible reduction of protons using complex metal clusters with unusual ligands. The catalytic center of the [FeFe]-hydrogenases is called the H-cluster, and is characterized by a [4Fe-4S] cluster connected via a cysteine thiolate to a 2Fe subcluster coordinated by carbon monoxide and cyanide ligands as well as a bridging dithiolate. Assembly of the H-cluster is carried out by three hydrogenase maturation proteins: HydE, HydF, and HydG. HydF is a GTPase and has been implicated to serve as a scaffold for assembly of the 2Fe subcluster of the H-cluster. HydE and HydG are radical S-adenosylmethionine (SAM) enzymes and thus are thought to utilize reductive cleavage of SAM to initiate radical chemistry. HydG has been shown to catalyze the formation of the carbon monoxide and cyanide ligands of the H-cluster utilizing tyrosine as a substrate. HydE, therefore, has been proposed to be responsible for biosynthesis of the dithiolate ligand of the H-cluster. The aim of this study was to biochemically characterize active, Fe-S reconstituted HydE and to identify the substrate of this radical SAM enzyme. Questions to be studied also included studying the role of HydE in H-cluster maturation. This study used protein purified from recombinant E. coli. The purified protein was chemically reconstituted with iron and sulfide, and used for spectroscopic characterization and HPLC based activity assays. Colorimetric assays were also used for protein characterization and to test for the consumption of substrate. The results indicate that cysteine is likely the substrate of HydE. Activity assays show that HydE- catalyzed SAM cleavage is stimulated in the presence of cysteine, and HydF purified from different genetic backgrounds shows a spectroscopic shift in the lambda max when both HydE and cysteine are present during growth. Spectroscopic characterization confirms that HydE is an Fe-S containing radical SAM enzyme and that cysteine may be a substrate during [FeFe]-hydrogenase H-cluster maturation.

Farah, Sahar. "Identification of Rho-associated protein kinaseÃ as an insulin receptor substrate-1 binding protein". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ28422.pdf.

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Cheng, Kai. "Identification of Pctaire1 as a p35-interacting protein and a novel substrate for Cdk5 /". View Abstract or Full-Text, 2003. http://library.ust.hk/cgi/db/thesis.pl?BICH%202003%20CHENG.

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Thesis (Ph. D.)--Hong Kong University of Science and Technology, 2003.
Includes bibliographical references (leaves 153-177). Also available in electronic version. Access restricted to campus users.

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Libri sul tema "Substrate identification":

Ellis, Martin B. Microfungi on miscellaneous substrates: An identification handbook. Portland, Or: Timber Press, 1988.

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Ellis, Martin B. Microfungi on miscellaneous substrates: An identification handbook. Slough, England: Richmond Pub. Co., 1998.

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Yang, Li, Amin Rida e Manos M. Tentzeris. Design and Development of Radio Frequency Identification (RFID) and RFID-Enabled Sensors on Flexible Low Cost Substrates. Cham: Springer International Publishing, 2009. http://dx.doi.org/10.1007/978-3-031-02524-2.

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Li, Yang. Design and development of radio frequency identification (RFID) and RFID-enabled sensors on flexible low cost substrates. San Rafael, Calif. (1537 Fourth Street, San Rafael, CA 94901 USA): Morgan & Claypool Publishers, 2009.

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Shao, Jiahong. Identification of peptide substrates of calcium-dependent protein kinase from random peptide phage display libraries and phosphorylation studies of the peptide substrate in transgenic tobacco cells. 1999.

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Hemming, Matthew L. Identification of [beta]-secretase (BACE1) substrates using quantitative proteomics. 2012.

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Ellis, Paul D. *. Synaptic tyrosine kinase: partial characterization and identification of endogenous substrates. 1988.

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Lang, Helen. Embodied or Ensouled. Oxford University Press, 2017. http://dx.doi.org/10.1093/acprof:oso/9780190490447.003.0003.

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This paper begins with the problem of natural substance and its identification by Aristotle as the combination of form and matter, as distinct from the substrate of the body. This is an investigation of the relation between the combination of form and matter on the one hand and body on the other. Looking at both natural science and metaphysics will give us a clear account of the partners involved in the relationship that defines living things. That is the first step toward understanding how and why they enter into a relation and exactly what, for Aristotle, is entailed by that relation. Being clear on the relation of natural substance in the sense of the combination of form and matter, soul and body, will in turn allow us to ask if soul is in every regard attached to body or if there is “something proper” to soul alone.

Waldek, Stephen. Fabry disease. A cura di Neil Turner. Oxford University Press, 2018. http://dx.doi.org/10.1093/med/9780199592548.003.0335_update_001.

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Fabry disease is a rare X-linked disorder of glycosphingolipid metabolism caused by a deficiency of the lysosomal acid hydrolase enzyme, alpha-galactosidase A. The resulting accumulation of substrate, mostly globotriaosylceramide, leads to a progressive, multiorgan disease affecting predominantly the kidneys, skin, heart, and nervous system. It is one of over 50 lysosomal storage diseases. It is typically diagnosed in young men after many years of ‘acral pain’ syndrome, when the diagnosis is made through identification of characteristic abnormalities of skin, kidney or heart, or of other organs. Renal failure has been a common outcome. Females may also develop manifestations, usually later in life. Renal biopsy shows vacuoles/deposits in podocytes and other renal cell types with progressive scarring. The diagnosis can be made by measuring enzyme levels in men, or by genetic testing. This latter is the more reliable test in women. Fabry disease can now be treated where affordable by regular (every 2 weeks) intravenous infusions of recombinant preparations of the deficient enzyme. These are burdensome and expensive, but are transforming the outlook for the condition.

Piau, Angela Yeming. Global approach toward the identification of Transcription factor substrates of F-Box in S. cerevisiae. 2009.

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Grimaldi, Giovanna, Giuliana Catara, Carmen Valente e Daniela Corda. "In Vitro Techniques for ADP-Ribosylated Substrate Identification". In Methods in Molecular Biology, 25–40. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8588-3_3.

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Nagy, Szilvia K., e Tamás Mészáros. "In Vitro Translation-Based Protein Kinase Substrate Identification". In Methods in Molecular Biology, 231–43. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-782-2_15.

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Ketelaar, KassaDee J., e Ian S. Wallace. "Methods for Large-Scale Identification of Protein Kinase Substrate Networks". In Kinomics, 255–80. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2015. http://dx.doi.org/10.1002/9783527683031.ch11.

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Elu, Nagore, Natalia Presa e Ugo Mayor. "RNAi-Based Screening for the Identification of Specific Substrate-Deubiquitinase Pairs". In The Ubiquitin Code, 95–105. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2859-1_7.

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Powers, Brendan L., Hana Hall, Harry Charbonneau e Mark C. Hall. "A Substrate Trapping Method for Identification of Direct Cdc14 Phosphatase Targets". In Methods in Molecular Biology, 119–32. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6502-1_10.

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Seghetti, Paolo, Niccolò Biasi, Matteo Mercati, Valentina Hartwig, Andrea Rossi, Marco Laurino e Alessandro Tognetti. "Identification of the Arrhythmogenic Substrate in Brugada Syndrome: A Computational Study". In IFMBE Proceedings, 513–20. Cham: Springer Nature Switzerland, 2024. http://dx.doi.org/10.1007/978-3-031-49068-2_52.

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Shinbane, Jerold S., Leslie A. Saxon, Rahul N. Doshi, Philip M. Chang e Matthew J. Budoff. "CCTA Cardiac Electrophysiology Applications: Substrate Identification, Virtual Procedural Planning, and Procedural Facilitation". In Cardiac CT Imaging, 455–86. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-28219-0_24.

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Schlossmann, Jens, e Stefanie Wolfertstetter. "Identification of cCMP and cUMP Substrate Proteins and Cross Talk Between cNMPs". In Non-canonical Cyclic Nucleotides, 149–67. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/164_2015_38.

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Stintzi, Annick, Nils Stührwohldt, Stefanie Royek e Andreas Schaller. "Identification of Cognate Protease/Substrate Pairs by Use of Class-Specific Inhibitors". In Methods in Molecular Biology, 67–81. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2079-3_6.

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Freney, J., P. Laban, M. Desmonceaux, H. Alexandre, B. Poggi, J. P. Gayral e J. Fleurette. "An Automatic Micromethod for the Identification of Gram-Negative Bacilli by Carbon Substrate Assimilation Tests". In Rapid Methods and Automation in Microbiology and Immunology, 377–89. Berlin, Heidelberg: Springer Berlin Heidelberg, 1985. http://dx.doi.org/10.1007/978-3-642-69943-6_48.

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Atti di convegni sul tema "Substrate identification":

roffey, jon, Andrew Turnbull, Christian Dillon, Susan Boyd, Philippe Riou, Mark Linch, Peter Parker, Sven Kjaer e Neil McDonald. "Abstract LB-052: Kinase identification of proximal substrates (KIPS): A novel chemical genetics approach for kinase substrate identification". In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-lb-052.

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Pendell Jones, Jay, Nicholas F. Fell, Jr., Troy A. Alexander, Kristl Dorschner, Christin Tombrello, B. Ritz Reis e Augustus W. Fountain III. "Surface-enhanced Raman substrate optimization for bacterial identification". In AeroSense 2003, a cura di Edward M. Carapezza. SPIE, 2003. http://dx.doi.org/10.1117/12.486987.

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Pittol, José A., Yamitet Sánchez, Rosalba Lamanna, Silvana Revollar e Pastora Vega. "A Fuzzy Virtual Sensor for Substrate Concentration in a Wastewater Treatment Plant". In Computational Intelligence and Bioinformatics / Modelling, Simulation, and Identification. Calgary,AB,Canada: ACTAPRESS, 2012. http://dx.doi.org/10.2316/p.2012.755-058.

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Lytle, Fred E. "Identification of Bacterial Pathogens by Laser Excited Fluorescence". In Laser Applications to Chemical Analysis. Washington, D.C.: Optica Publishing Group, 1987. http://dx.doi.org/10.1364/laca.1987.ma7.

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Aminopeptidase profiling is an established bioanalytical technique used for the identification of bacterial pathogens. This method is based on the production of highly fluorescent β-naphthylamine (BNA) through aminopeptidase hydrolysis of a series of nonfluorescent L-aminoacid-β-naphthylamide substrates by the microorganism of interest. The resultant profiles used to make the identification are bar graphs of the extent of hydrolysis of the substrate versus the identity of the nutrient. The shape of the plot is indicative of the pathogen.

Djerafi, Tarek, Ke Wu, Alexandre Marque e Anthony Ghiotto. "Chipless substrate integrated waveguide tag for millimeter wave identification". In 2015 Global Symposium On Millimeter Waves (GSMM). IEEE, 2015. http://dx.doi.org/10.1109/gsmm.2015.7175466.

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Cong Lu, Fengjun Zhang, Ying Lu, Yi Liu e Shuang Zhong. "Screening and identification of aerobic denitrifier with nitrite as substrate". In 2011 Second International Conference on Mechanic Automation and Control Engineering (MACE). IEEE, 2011. http://dx.doi.org/10.1109/mace.2011.5987725.

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Jiao, Mingmin, Xiaohong Li e Enmin Feng. "Parameter Identification in Microbial Continuous Fermentation with Intracellular Substrate and Products". In 2012 International Conference on Control Engineering and Communication Technology (ICCECT). IEEE, 2012. http://dx.doi.org/10.1109/iccect.2012.94.

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Francioso, Luca, Pietro Siciliano, Robert Bjorklund e Tina Kratz Rulcker. "Silicon substrate microelectrodes voltammetry performances in white wine faults identification and quantification". In 2007 IEEE Sensors. IEEE, 2007. http://dx.doi.org/10.1109/icsens.2007.4388568.

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Drabison, Thomas, Mike Boeckman, Eric Eisenmann, Sharyn D. Baker, Shuiying Hu, Alex Sparreboom e Zahra Talebi. "Identification of Pazopanib as an OATP1B1 Substrate from a Competitive Counterflow Screen". In ASPET 2023 Annual Meeting Abstracts. American Society for Pharmacology and Experimental Therapeutics, 2023. http://dx.doi.org/10.1124/jpet.122.555040.

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Rapporti di organizzazioni sul tema "Substrate identification":

Tsvetkov, Lyuben. Identification of New Chk2 Substrates. Fort Belvoir, VA: Defense Technical Information Center, luglio 2002. http://dx.doi.org/10.21236/ada410391.

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Dijkgraaf, Gerrit J., e Zena Werb. Identification of MMP Substrates in the Mammary Gland. Fort Belvoir, VA: Defense Technical Information Center, luglio 2005. http://dx.doi.org/10.21236/ada443693.

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Dijkgraaf, Gerrit J., e Zena Werb. Identification of MMP Substrates in the Mammary Gland. Fort Belvoir, VA: Defense Technical Information Center, luglio 2004. http://dx.doi.org/10.21236/ada430554.

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Dijkgraaf, Gerrit J. Identification of MMP Substrates in the Mammary Gland. Fort Belvoir, VA: Defense Technical Information Center, luglio 2003. http://dx.doi.org/10.21236/ada423278.

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Azem, Abdussalam, George Lorimer e Adina Breiman. Molecular and in vivo Functions of the Chloroplast Chaperonins. United States Department of Agriculture, giugno 2011. http://dx.doi.org/10.32747/2011.7697111.bard.

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We present here the final report for our research project entitled "The molecular and in vivo functions of the chloroplast chaperonins”. Over the past few decades, intensive investigation of the bacterial GroELS system has led to a basic understanding of how chaperonins refold denatured proteins. However, the parallel is limited in its relevance to plant chaperonins, since the plant system differs from GroEL in genetic complexity, physiological roles of the chaperonins and precise molecular structure. Due to the importance of plant chaperonins for chloroplast biogenesis and Rubisco assembly, research on this topic will have implications for many vital applicative fields such as crop hardiness and efficiency of plant growth as well as the production of alternative energy sources. In this study, we set out to investigate the structure and function of chloroplast chaperonins from A. thaliana. Most plants harbor multiple genes for chaperonin proteins, making analysis of plant chaperonin systems more complicated than the GroEL-GroES system. We decided to focus on the chaperonins from A. thaliana since the genome of this plant has been well defined and many materials are available which can help facilitate studies using this system. Our proposal put forward a number of goals including cloning, purification, and characterization of the chloroplast cpn60 subunits, antibody preparation, gene expression patterns, in vivo analysis of oligomer composition, preparation and characterization of plant deletion mutants, identification of substrate proteins and biophysical studies. In this report, we describe the progress we have made in understanding the structure and function of chloroplast chaperonins in each of these categories.

Akli, Said. Identification of New Substrates for Breast Tumor Specific LMW Cyclin E/CDK2 Kinase. Fort Belvoir, VA: Defense Technical Information Center, settembre 2012. http://dx.doi.org/10.21236/ada570588.

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Akli, Said. Identification of New Substrates for Breast Tumor-Specific LMW Cyclin E/CDk2 Kinase. Fort Belvoir, VA: Defense Technical Information Center, settembre 2011. http://dx.doi.org/10.21236/ada560522.

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Granot, David, Scott Holaday e Randy D. Allen. Enhancing Cotton Fiber Elongation and Cellulose Synthesis by Manipulating Fructokinase Activity. United States Department of Agriculture, 2008. http://dx.doi.org/10.32747/2008.7613878.bard.

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a. Objectives (a) Identification and characterization of the cotton fiber FRKs; (b) Generating transgenic cotton plants overproducing either substrate inhibited tomato FRK or tomato FRK without substrate inhibition; (c) Generating transgenic cotton plants with RNAi suppression of fiber expressed FRKs; (d) Generating Arabidopsis plants that over express FRK1, FRK2, or both genes, as additional means to assess the contribution of FRK to cellulose synthesis and biomass production. b. Background to the topic: Cellulose synthesis and fiber elongation are dependent on sugar metabolism. Previous results suggested that FRKs (fructokinase enzymes that specifically phosphorylate fructose) are major players in sugar metabolism and cellulose synthesis. We therefore hypothesized that increasing fructose phosphorylation may enhance fiber elongation and cellulose synthesis in cotton plants. Accordinlgy, the objectives of this research were: c. Major conclusions and achievements: Two cotton FRKs expressed in fibers, GhFRK2 and GhFRK3, were cloned and characterized. We found that GhFRK2 enzyme is located in the cytosol and GhFRK3 is located within plastids. Both enzymes enable growth on fructose (but not on glucose) of hexose kinase deficient yeast strain, confirming the fructokinase activity of the cloned genes. RNAi constructs with each gene were prepared and sent to the US collaborator to generate cotton plants with RNAi suppression of these genes. To examine the effect of FRKs using Arabidopsis plants we generated transgenic plants expressing either LeFRK1 or LeFRK2 at high level. No visible phenotype has been observed. Yet, plants expressing both genes simultaneously are being created and will be tested. To test our hypothesis that increasing fructose phosphorylation may enhance fiber cellulose synthesis, we generated twenty independent transgenic cotton plant lines overexpressing Lycopersicon (Le) FRK1. Transgene expression was high in leaves and moderate in developing fiber, but enhanced FRK activity in fibers was inconsistent between experiments. Some lines exhibited a 9-11% enhancement of fiber length or strength, but only one line tested had consistent improvement in fiber strength that correlated with elevated FRK activity in the fibers. However, in one experiment, seed cotton mass was improved in all transgenic lines and correlated with enhanced FRK activity in fibers. When greenhouse plants were subjected to severe drought during flowering and boll development, no genotypic differences in fiber quality were noted. Seed cotton mass was improved for two transgenic lines but did not correlate with fiber FRK activity. We conclude that LeFRK1 over-expression in fibers has only a small effect on fiber quality, and any positive effects depend on optimum conditions. The improvement in productivity for greenhouse plants may have been due to better structural development of the water-conducting tissue (xylem) of the stem, since stem diameters were larger for some lines and the activity of FRK in the outer xylem greater than observed for wild-type plants. We are testing this idea and developing other transgenic cotton plants to understand the roles of FRK in fiber and xylem development. We see the potential to develop a cotton plant with improved stem strength and productivity under drought for windy, semi-arid regions where cotton is grown. d. Implications, scientific and agricultural: FRKs are probably bottle neck enzymes for biomass and wood synthesis and their increased expression has the potential to enhance wood and biomass production, not only in cotton plants but also in other feed and energy renewable plants.

Mizrach, Amos, Michal Mazor, Amots Hetzroni, Joseph Grinshpun, Richard Mankin, Dennis Shuman, Nancy Epsky e Robert Heath. Male Song as a Tool for Trapping Female Medflies. United States Department of Agriculture, dicembre 2002. http://dx.doi.org/10.32747/2002.7586535.bard.

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This interdisciplinaray work combines expertise in engineering and entomology in Israel and the US, to develop an acoustic trap for mate-seeking female medflies. Medflies are among the world's most economically harmful pests, and monitoring and control efforts cost about $800 million each year in Israel and the US. Efficient traps are vitally important tools for medfly quarantine and pest management activities; they are needed for early detection, for predicting dispersal patterns and for estimating medfly abundance within infested regions. Early detection facilitates rapid response to invasions, in order to contain them. Prediction of dispersal patterns facilitates preemptive action, and estimates of the pests' abundance lead to quantification of medfly infestations and control efforts. Although olfactory attractants and traps exist for capturing male and mated female medflies, there are still no satisfactorily efficient means to attract and trap virgin and remating females (a significant and dangerous segment of the population). We proposed to explore the largely ignored mechanism of female attraction to male song that the flies use in courtship. The potential of such an approach is indicated by studies under this project. Our research involved the identification, isolation, and augmentation of the most attractive components of male medfly songs and the use of these components in the design and testing of traps incorporating acoustic lures. The project combined expertise in acoustic engineering and instrumentation, fruit fly behavior, and integrated pest management. The BARD support was provided for 1 year to enable proof-of-concept studies, aimed to determine: 1) whether mate-seeking female medflies are attracted to male songs; and 2) over what distance such attraction works. Male medfly calling song was recorded during courtship. Multiple acoustic components of male song were examined and tested for synergism with substrate vibrations produced by various surfaces, plates and loudspeakers, with natural and artificial sound playbacks. A speaker-funnel system was developed that focused the playback signal to reproduce as closely as possible the near-field spatial characteristics of the sounds produced by individual males. In initial studies, the system was tasted by observing the behavior of females while the speaker system played songs at various intensities. Through morning and early afternoon periods of peak sexual activity, virgin female medflies landed on a sheet of filter paper at the funnel outlet and stayed longer during broadcasting than during the silent part of the cycle. In later studies, females were captured on sticky paper at the funnel outlet. The mean capture rates were 67 and 44%, respectively, during sound emission and silent control periods. The findings confirmed that female trapping was improved if a male calling song was played. The second stage of the research focused on estimating the trapping range. Initial results indicated that the range possibly extended to 70 cm, but additional, verification tests remain to be conducted. Further studies are planned also to consider effects of combining acoustic and pheromonal cues.

Sessa, Guido, e Gregory Martin. MAP kinase cascades activated by SlMAPKKKε and their involvement in tomato resistance to bacterial pathogens. United States Department of Agriculture, gennaio 2012. http://dx.doi.org/10.32747/2012.7699834.bard.

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The research problem: Pseudomonas syringae pv. tomato (Pst) and Xanthomonas campestrispv. vesicatoria (Xcv) are the causal agents of tomato bacterial speck and spot diseases, respectively. These pathogens colonize the aerial parts of the plant and cause economically important losses to tomato yield worldwide. Control of speck and spot diseases by cultural practices or chemicals is not effective and genetic sources of resistance are very limited. In previous research supported by BARD, by gene expression profiling we identified signaling components involved in resistance to Xcvstrains. Follow up experiments revealed that a tomato gene encoding a MAP kinase kinase kinase (MAPKKKe) is required for resistance to Xcvand Pststrains. Goals: Central goal of this research was to investigate the molecular mechanisms by which MAPKKKεand associated MAP kinase cascades regulate host resistance. Specific objectives were to: 1. Determine whether MAPKKKεplays a broad role in defense signaling in plants; 2. Identify components of MAP kinase cascades acting downstream of MAPKKKε; 3. Determine the role of phosphorylation-related events in the function of MAPKKKε; 4. Isolate proteins directly activated by MAPKKKε-associatedMAPK modules. Our main achievements during this research program are in the following major areas: 1. Characterization of MAPKKKεas a positive regulator of cell death and dissection of downstream MAP kinase cascades (Melech-Bonfil et al., 2010; Melech-Bonfil and Sessa, 2011). The MAPKKKεgene was found to be required for tomato resistance to Xcvand Pstbacterial strains and for hypersensitive response cell death triggered by different R gene/effector gene pairs. In addition, overexpression analysis demonstrated that MAPKKKεis a positive regulator of cell death, whose activity depends on an intact kinase catalytic domain. Epistatic experiments delineated a signaling cascade downstream of MAPKKKεand identified SIPKK as a negative regulator of MAPKKKε-mediated cell death. Finally, genes encoding MAP kinase components downstream of MAPKKKεwere shown to contribute to tomato resistance to Xcv. 2. Identification of tomato proteins that interact with MAPKKKεand play a role in plant immunity (Oh et al., 2011). We identified proteins that interact with MAPKKKε. Among them, the 14-3-3 protein TFT7 was required for cell death mediated by several R proteins. In addition, TFT7 interacted with the MAPKK SlMKK2 and formed homodimersin vivo. Thus, TFT7 is proposed to recruit SlMKK2 and MAPKKK client proteins for efficient signal transfer. 3. Development of a chemical genetic approach to identify substrates of MAPKKKε-activated MAP kinase cascades (Salomon et al., 2009, 2011). This approach is based on engineering the kinase of interest to accept unnatural ATP analogs. For its implementation to identify substrates of MAPKKKε-activated MAP kinase modules, we sensitized the tomato MAP kinase SlMPK3 to ATP analogs and verified its ability to use them as phosphodonors. By using the sensitized SlMPK3 and radiolabeled N6(benzyl)ATP it should be possible to tag direct substrates of this kinase. 4. Development of methods to study immunity triggered by pathogen-associated molecular patterns (PAMPs) in tomato and N. benthamiana plants (Kim et al., 2009; Nguyen et al. 2010). We developed protocols for measuring various PTI-associatedphenotypes, including bacterial populations after pretreatment of leaves with PAMPs, induction of reporter genes, callose deposition at the cell wall, activation of MAP kinases, and a luciferase-based reporter system for use in protoplasts. Scientific and agricultural significance: Our research activities discovered and characterized a signal transduction pathway mediating plant immunity to bacterial pathogens. Increased understanding of molecular mechanisms of immunity will allow them to be manipulated by both molecular breeding and genetic engineering to produce plants with enhanced natural defense against disease. In addition, we successfully developed new biochemical and molecular methods that can be implemented in the study of plant immunity and other aspects of plant biology.