Tesi: "Schizosaccharomyces pombe"

1

Wells, Jennifer. "Schizosaccharomyces pombe meiotic linear elements". Thesis, Bangor University, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.432058.

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2

Dhamija, Sunder Sham. "Alkaline phosphatases in Schizosaccharomyces pombe /". [S.l : s.n.], 1987. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.

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3

Pereira, Paulo. "Control of mating in Schizosaccharomyces pombe". Thesis, University College London (University of London), 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271226.

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4

Cuenca, Liliana. "Repeated batch cultivation of Schizosaccharomyces pombe". Thesis, University of Surrey, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.441865.

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5

McDougall, Rachel Clare. "Schizosaccharomyces pombe : from sequence to function". Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627369.

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6

Ludin, Katja Maria. "Adenine regulated genes in Schizosaccharomyces pombe /". [S.l.] : [s.n.], 1994. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.

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7

Scott, Daniel Dehany. "Characterisation of RNA uridylyltransferases in Schizosaccharomyces pombe". Thesis, University of Oxford, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.669940.

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The control of RNA stability and function via 3' end modification is a widely conserved and biologically important regulatory mechanism. Though this control has traditionally been considered to depend entirely on the addition or removal of long or short poly(A) tails, in recent years the post-transcriptional addition of uridylyl residues to the 3' ends of diverse RNAs has been identified as a physiologically relevant degradative signal in a range of different species. The Schizosaccharomyces pombe protein Cid1 has been previously shown to possess uridylylation activity on mRNAs and via this uridylylation to promote the decapping and degradation of mRNAs. This work investigates the presence of residual uridylylation activity in strains lacking the cid1 uridylyltransferase and shows that a second S. pombe enzyme, cid16, possesses robust and highly specific poly(U) polymerase (PUP) activity in vitro and is able to uridylylate the act1 mRNA in vivo Characterisation of Cid16 shows that it possesses greater processivity and selectivity than Cid1 both for RNA substrates and for UTP, suggesting that Cid16 is a more stringent PUP than is Cid1. Deletion of cid16 causes few changes in mRNA levels during exponential growth, suggesting that cid16 may act on other targets or during other phases of the S. pombe life cycle, including a potential role in the regulation of RNAi factors during meiosis. Separate experiments show that, while cid1 is dispensable for the stability of most RNAs during exponential growth, it unexpectedly regulates the transcription of genes in extended subtelomeric regions of the S. pombe genome, suggesting a hitherto unknown role for cid1 in the regulation of subtelomeric heterochromatin formation and/or propagation. These observations suggest that the process of uridylylation in S. pombe is significantly more complicated than previously suspected and may regulate a range of different targets in diverse biological pathways.

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8

Walters, Nicola Jane. "Arginine and proline catabolism in Schizosaccharomyces pombe". Thesis, University of Cambridge, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.257192.

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9

Murton, Heather Elizabeth. "Regulation of LTR retrotransposons in Schizosaccharomyces pombe". Thesis, University of Newcastle Upon Tyne, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.606815.

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The genome of the fission yeast Schizosaccharomyces pombe is host to a family of long terminal repeat (LTR) retrotransposons called Tf2, which is comprised of 13 full length elements and around 250 solo LTRs. The expression of these elements is subjected to chromatin based silencing during normal growth conditions but is induced in response to hypoxia via the action of Sre1 , a homologue of human SREBPtranscription factor. The aim of this study was to dissect the mechanisms that regulate Tf2 expression and to determine how they contribute to the control of element mobilisation. Characterisation of the Tf2 LTR element identified sequences that mediate its transcriptional control, while further studies identified roles for the Spt6 and Asf1 histone chaperones in Tf2 element silencing. Furthermore, similar transcription controls were shown operate at the related Tf1 LTR retrotransposons that are present in the genomes of other wild type s. pombe strains. To analyse the effects of transcriptional regulation upon Tf2 element propagation, an assay was established to measure the mobilisation frequency of a marked endogenous Tf2 element (Tf2-12natAI). Using this system the affect of key transcriptional regulators upon Tf2 mobilisation frequency was determined. The HIRA nucleosome assembly complex has previously been shown to be required for Tf2 silencing and accordingly element expression was found to be increased >10-fold in the absence of HIRA. Despite this finding only a marginal (1.8-fold) increase in the frequency of Tf2 mobilisation was observed. In contrast, expression of a constitutively active form of the transcription factor Sre1 (Sre1-N) resulted in a large increase in mobilisation (>10-fold) despite inducing Tf2 expression to less than 50% of that observed in the absence of HIRA. Furthermore, loss of the CENP-B homologue, Abp1 resulted in only a small increase in Tf2 expression (3-fold) but a significant increase in mobilisation (4-fold). Therefore, mobilisation frequency is not necessarily correlated with expression levels, suggesting that Tf2 mobilisation is subjected to additional controls. These studies also revealed that the Asf1 histone chaperone restricts Tf2 element propagation, while components of the RNAi pathway promote Tf2 mobilisation. In an effort to identify novel regulators of Tf2 expression, the homologues of the s. cerevisae bromodomain containing AAA-ATPase Yta7 were characterised. s. pombe contains two Yta7 homologues (SPAC31G5.19and SPBP22H7.05c)which were named Yta71 and Yta72 respectively. Yta71, but not Yta72, was found to restrict the expression of Tf2 and Tfi elements and their solo LTRs. Deletion of yta71+ was also found to result in sensitivity to the spindle poison thiabendazole and an increased level of chromosome loss. Consistent with these findings Yta71 is required for the integrity of centromeric heterochromatin and also the heterochromatin associated with the mating type (mat) locus. Thus Yta71 is required for transcriptional silencing at multiple loci in fission yeast.

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10

Jenkins, Blair. "Mapping zinc-responsive elements in Schizosaccharomyces pombe". The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1338160105.

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11

Winters, Lora. "Mechanism of spindle assembly in Schizosaccharomyces pombe-". Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-225764.

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At the onset of cell division microtubules growing from spindle pole bodies (SPB) interact with each other to form the mitotic spindle enabling proper chromosome positioning and segregation. However, the exact mechanism of microtubule dynamics and microtubule associated proteins (MAPs) underlying spindle assembly is still not well understood. We developed an in vivo method to observe spindle assembly in the fission yeast Schizosaccharomyces pombe by inducing depolymerization of already formed and grown spindles by subjecting the cells to low temperatures, followed by subsequent repolymerization at a permissive temperature. We observed that microtubules pivot, i.e., perform angular movement around the SPB in a random manner, exploring the intranuclear space. Eventually microtubules extending from opposite SPBs come into contact and establish an antiparallel connection thus reassembling the spindle. Mutant approaches revealed that deletion of ase1 and klp5 did not prevent spindle reassembly, however introduced aberrations during the spindle formation. Amazingly, cut7p showed direct colocalization with microtubule overlap during spindle reassembly. Abrogation of cut7p led to inability to form a functional spindle. Thus, cut7p is the main regulator of spindle formation in fission yeast. None of the mutant strains affected microtubule pivoting, confirming that microtubule pivoting is a random movement unrelated to MAPs.

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12

Sherman, Daniel A. "Assembly of the Schizosaccharomyces pombe MCM complex /". Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 1999. http://wwwlib.umi.com/cr/ucsd/fullcit?p9951421.

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13

Fankhauser, Hans. "Regulation of thiamine metabolisme in Schizosaccharomyces pombe /". [S.l : s.n.], 1995. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.

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14

Walfridsson, Julian. "The CHD chromatin remodeling factors in schizosaccharomyces pombe /". Stockholm, 2007. http://diss.kib.ki.se/2007/978-91-7357-106-7/.

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15

Spåhr, Henrik. "The transcription machinery in Schizosaccharomyces pombe and its regulation /". Stockholm, 2004. http://diss.kib.ki.se/2004/91-7349-863-7/.

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16

Stevenson, Abigail Louise. "Cytoplasmic polyadenylation in S. pombe". Thesis, University of Oxford, 2005. http://ora.ox.ac.uk/objects/uuid:4ef2e3ad-8bac-44b5-b838-4023b18c4693.

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Cid1 is a cytoplasmic member of a novel class of regulatory poly(A) polymerases discovered recently in yeast, worms and vertebrates. Previous genetic studies in the fission yeast, Schizosaccharomyces pombe, suggested a role for Cid1 in the checkpoint response to replication stress, but it was not known how a poly(A) polymerase might contribute to this response. Further investigations into the mode of action of Cid1 were therefore undertaken in this study. Cid1 is likely to target specific RNAs for polyadenylation; potential RNA substrates were identified using the complementary methods of microarray hybridisation and whole proteome analysis using two-dimensional liquid chromatography. These experiments revealed that Cid1 does not affect RNAs during normal, unperturbed growth but instead alters the expression of specific subsets of genes during replication stress. Many RNAs affected by Cid1 in these circumstances were cell-cycle dependent and telomeric transcripts, including those encoding histones and a novel RecQ helicase, Rqh2. As Cid1 lacks an RNA recognition motif, it is unlikely to bind selectively to RNA targets on its own. Cid1-interacting proteins were identified using yeast two-hybrid and tandem affinity purification methods. From these studies, novel members of a Cid1 complex have been discovered including: a previously uncharacterised metallo-beta-lactamase, RNA-binding proteins, ribosomal proteins and a telomere-binding protein. Together, these approaches are leading to a model for the role of cytoplasmic polyadenylation by Cid1 in checkpoint control.

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17

Lützelberger, Martin. "Prä-mRNA Splicing in der Spalthefe Schizosaccharomyces pombe: In-vivo-Charakterisierung der Funktion des srp2 Gens". [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=958485690.

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18

Vietmeier-Decker, Corina. "Die Funktion des Mikrotubuli-assoziierten S. pombe Proteins Mal3p in der Mitose". [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=971864985.

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19

Simm, Claudia. "Das Metallothionein Zym1 aus Schizosaccharomyces pombe: Untersuchungen zur Funktion des Metallothioneins in einem phytochelatin-bildenden Organismus". [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=97560208X.

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20

Tuzon, Creighton T. "Telomeric chromatin structure and function in Schizosaccharomyces pombe /". Connect to full text via ProQuest. IP filtered, 2005.

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Thesis (Ph.D. in Biochemistry) -- University of Colorado at Denver and Health Sciences Center, 2005.
Typescript. Includes bibliographical references (leaves 93-103). Free to UCDHSC affiliates. Online version available via ProQuest Digital Dissertations;

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21

Kettner, Karina. "Optimierung von Schizosaccharomyces pombe für die heterologe Genexpression". Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2005. http://nbn-resolving.de/urn:nbn:de:swb:14-1118159087587-64313.

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Die vorliegende Arbeit beschäftigt sich mit der genetischen Optimierung der Spalthefe S. pombe für die biotechnologische Produktion von Fremdproteinen. Hierbei werden vor allem zwei Aspekte näher untersucht, zum einen die Stabilität des zu produzierenden Proteins und zum anderen die Bildung von Disulfidbrücken. Von anderen Organismen ist bekannt, dass die N-terminale AS im Verbund mit einem Lysinrest ein Protein destabilisieren kann. Das Modellprotein vVEGF besitzt an Position 2 einen Lysinrest (K2) und damit ein Hauptmerkmal eines derartigen Destabilisierungselementes. Falls das Protein dem Ubiquitin-vermittelten Abbau unterliegt, ist es wahrscheinlich, dass K2 eine essenzielle Rolle für die Stabilität dieses Proteins spielt. Im Rahmen dieser Arbeit konnte gezeigt werden, dass K2 in S. cerevisiae destabilisierend wirkt, während es in S. pombe keinen destabilisierenden Effekt hat. Dieses Ergebnis spricht dafür, dass es Unterschiede im Ubiquitin-vermittelten Abbau von Proteinen in diesen beiden Hefen gibt. Der Schwerpunkt dieser Arbeit lag auf der Analyse und Optimierung der Bildung von Disulfidbrücken in S. pombe. Disulfidbrücken stellen eines der wichtigsten Elemente der korrekten Proteinfaltung dar und werden in Eukaryonten vorwiegend im oxidierenden Milieu des ER in das naszierende Protein eingeführt. Aus diesem Grunde wurden Proteindisulfid-isomerasen (PDIs) und ER-oxidoreduktin (Ero)-ähnliche Proteine, die die Schlüssel-komponenten der Bildung von Disulfidbrücken in Eukaryonten darstellen, näher untersucht. In S. pombe finden sich insgesamt drei PDI-Homologe (SpPdi1p, SpPdi2p und SpPdi3p) sowie zwei Ero-Homologe (SpEro1a p und SpEro1b p). Mit Ausnahme des nicht glycosylierten SpPdi2p, sind alle Proteine Membran-assoziierte glycosylierte Komponenten des ER. SpPdi2p und SpPdi3p sowie SpEro1a p und SpEro1b p liegen in vivo teilweise in oxidiertem Zustand vor. Des Weiteren konnte gezeigt werden, dass SpEro1b p, nicht jedoch SpEro1a p in der Lage ist, die temperatursensitive S. cerevisiae ero1-1-Mutante funktionell zu komplementieren. Interessanterweise ergab die Untersuchung konservierter Cysteine mittels gerichteter Mutagenese einerseits Unterschiede zwischen SpEro1a p und SpEro1b p sowie andererseits zwischen den S. pombe Ero-Proteinen und den Ero-Proteinen anderer Spezies. Im Gegensatz zu Ero1b p wird Ero1a p durch reduzierenden Stress und Hitzestress induziert. Dies deutet darauf hin, dass SpEro1b p für die Bildung von Disulfidbrücken unter normalen Wachstumsbedingungen nötig ist, während SpEro1a p vornehmlich bei der Adaption der Zellen an Stressbedingungen erforderlich ist. Abschließend konnte gezeigt werden, dass die gesteigerte Expression von SpEro1a p und SpEro1b p zu einer deutlich erhöhten Ausbeute des disulfidhaltigen heterologen Proteins Orf19p-HA führt. Dieser Befund impliziert, dass in S. pombe die Oxidation der Disulfidbrücken für die Faltung von Proteinen vermutlich limitierend ist.

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22

Kristell, Carolina. "Chromatin Dynamics in the Fission Yeast, Schizosaccharomyces pombe". Doctoral thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-158084.

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In the eukaryotic cell nucleus, spatial organization and dynamics of the genome is important in the regulation of gene expression. This thesis describes the use of the fission yeast, Schizosaccharomyces pombe, to study chromatin regulation and dynamics. We used nitrogen starvation to induce transcription of genes in fission yeast cells. In induced genes, nucleosomes get evicted in both the promoter and in the open reading frame (ORF). In the genes with the highest expression more nucleosomes get evicted from the ORF than from the promoter. This indicates that large rearrangements of the chromatin are occurring during a drastic gene induction. Many of the genes that become expressed early after nitrogen starvation are located together in clusters. In a cell where nitrogen is present in the surrounding media the gene clusters locate close to the nuclear periphery. When the nitrogen source is removed from the media, the clusters move to a more internal position. Thus rearrangement of chromatin due to gene induction, described in the first study, is accompanied by subnuclear changes of localization. Another type of regulation is the silencing of genes. We have studied a factor necessary for correct repression of genes located in silent chromatin, in S. pombe. The protein, Clr2, is part of the SHREC complex containing a remodeler (Mit1) and a histone deacetylase (Clr3). By bioinformatic analysis of Clr2 and newly sequenced fungi genomes, three motifs were identified. To gather more information about important parts of the Clr2 protein, deletions were made. When removing from about 20 to 100 amino acids in the middle of the protein, silencing of a reporter gene inserted at the mating-type region, inner repeats of centromere 1 and at the central core of centromere 2, failed. This indicates that Clr2 has an important role in establishing silent chromatin.

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23

Forfar, Rachel Alice Emma. "Expression and Characterisation of GPCRs in Schizosaccharomyces pombe". Thesis, University of Warwick, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.490404.

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G protein-coupled receptors COPCRs) comprise the largest class of cell surface receptors and regulate a wide range of physiological processes in response to diverse stimuli. Accordingly, they are of great interest to the pharmaceutical industry, with -30% of marketed drugs targeting these receptors. However, the complex interplay between components of GPCR signalling pathways in mammalian cell systems inhibits the study of many aspects of these cascades. This thesis describes the use of Schizosaccharomyces p011lbe to simplify investigations into the expression and characterisation of both human and yeast GPCRs, and describes attempts to create a more sensitive strain for enhanced detection of signalling. Initially, the sxa2>lacZ reporter system was used to conduct studies of heterologously expressed AI and Aza adenosine receptors, but unfortunately these did not produce a functional response. Endeavours were made to increase receptor localisation at the plasma membrane by addition of a cleavable leader sequence, and to improve the interaction with the Oa subunit by exchanging IC3 loop from Mam2 into the adenosine receptors. However, no signalling was detected with these receptor alterations. . Additionally, a number of genetic modifications were made to the system in order to improve the detection of Ã‚Â·signalling. Modulation of the reporter strain pheromone response pathway was performed by creating a hyperactivated ras]Val17 strain, and this produced a two-fold increase in signalling through the endogenous Mam2 receptor. However, the ligand-independent signalling was also elevated, resulting in a similar signal:background ratio to the standard sxa2>lacZ reporter. Instead, simultaneous use of the lacZ and ura4 reporter genes in a 'double reporter' strain reduced ligand-independent levels of signalling, thereby substantially increasing the signal:background ratio. This was further enhanced through the addition of 6Azauracil, a potent inhibitor of Ura4 activity. With further alterations this strain could help detect signalling through the adenosine receptors. The dimerisation of two yeast GPCRs was investigated as a model for dimerisation of human receptors, since the adenosine receptors were not functional in Sz. p011lbe. Heterodimerisation of the related yeast receptors Mam2 and STE2 demonstrated an altered Oa-specificity compared to the respective homodimers, which may have implications in the signal transduction of human OPCR heterodimers. The results presented in this thesis should allow investigations to be extended into the dimerisation of human GPCRs in Sz. p011lbe and to determine the effects on their Gaspecificities.

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24

Taricani-Tejada, Lorena. "A tale of three proteins in Schizosaccharomyces pombe". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/NQ63458.pdf.

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25

Crowther, Daniel. "Cloning and characterization of Cpf1P from Schizosaccharomyces pombe". Thesis, University of Oxford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320634.

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26

Coxon, Angela. "Analysis of the cdc21'+ gene of Schizosaccharomyces pombe". Thesis, University of Oxford, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305370.

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27

Dunand-Sauthier, Isabelle. "Characterisation of the Schizosaccharomyces pombe sum 1'+ gene". Thesis, University of Oxford, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.365729.

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28

Bond, Michael Edward. "Ras signalling in the fission yeast Schizosaccharomyces pombe". Thesis, University of Warwick, 2012. http://wrap.warwick.ac.uk/46013/.

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Ras signalling is vital to many cellular processes. Ras proteins mediate a vast array of cellular signalling networks, and are conserved from humans to unicellular eukaryotes. The study of ras signalling in higher eukaryotes presents a number of technical challenges, due to the presence of multiple ras isoforms, regulatory proteins and activators. The fission yeast Sz. pombe represents an ideal system for the investigation of ras signalling, as it contains a single, nonessential ras protein (Ras1). In addition, Ras1 is involved in the regulation of a number of downstream pathways. A number of studies in recent years have highlighted the role of subcellular localisation in ras signalling output. The localisation of Ras1 in Sz. pombe has also been described as key in effector selection, with Ras1 at the plasma membrane regulating mating and Ras1 at the endomembranes regulating cell morphology. This thesis describes a series of studies utilising Ras1 mutants and chimeric Ras1 proteins which display differing localisation patterns to determine the role of Ras1 localisation in signalling. The data presented herein support the notion of a revised model for the role of Ras1 localisation in signalling, suggesting that the localisation of Ras1 to the plasma membrane is key to all signalling events downstream of Ras1. This thesis also describes the characterisation of oncogenic mutants of Ras1, demonstrating the importance of signalling magnitude in functional output. In addition, the importance of Ras1 regulation in cell viability and chromosome stability is also demonstrated. Finally, the functional expression of three human ras isoforms is described, validating the use of Sz. pombe as a model system for the heterologous expression of human ras signalling components.

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Dafydd, Heledd Fflur. "Functional characterisation of Schizosaccharomyces pombe meiotic linear elements". Thesis, Bangor University, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.536474.

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30

Amoah-Buahin, Evelyn. "Hyphal growth in the fission yeast Schizosaccharomyces pombe". Thesis, University of Sussex, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.430364.

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31

Curtis, Penelope Susan. "Studies on translation initiation factors in Schizosaccharomyces pombe". Thesis, University of Sussex, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285074.

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32

Redden, Matthew Wyatt. "Tandem repeats in the genome of Schizosaccharomyces pombe". Thesis, University of Exeter, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.410818.

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33

Spink, Jayne Marie. "The regulation of arginine breakdown in Schizosaccharomyces pombe". Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.385484.

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34

McLeod, Tina Louise. "Investigating methods of visualising translation in Schizosaccharomyces pombe". Thesis, University of Birmingham, 2016. http://etheses.bham.ac.uk//id/eprint/7105/.

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Gene expression is compartmentalised in eukaryotes due to the nuclear envelope separating the nuclear processes of transcription and pre-mRNA processing from cytoplasmic translation. While ribosomes are synthesised in the nucleus, it is understood that a number of mechanisms keep them inactive until they reach the cytoplasm, where they mature to become translation-competent. However, this consensus view is being challenged by a growing body of evidence in support of nuclear translation. A newly developed technique, known as ribopuromycylation (RPM), had reported the presence of puromycin-bound nascent peptides on immobilised ribosomes in the nuclei of human cells. I investigated whether this method could be used, combined with chromatin immunoprecipitation, to determine whether nuclear ribosomes can cotranscriptionally translate nascent transcripts in Schizosaccharomyces pombe. Surprisingly, I discovered that, in contrast to that reported in the original study, immobilising ribosomes with translation elongation inhibitors does not lead to retention of puromycylated peptides on ribosomes in either S. pombe, Drosophila melanogaster or HeLa cells. However, I show here preliminary data which suggest that despite puromycylated peptides being released from the ribosome, puromycin immunostaining might still be used to visualise the sub cellular localisation of ribosomes inS. pombe, along with other approaches which I also describe.

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35

McManus, John. "Structure of schizosaccharomyces pombe DNA in mouse cells". Thesis, University of Edinburgh, 1993. http://hdl.handle.net/1842/11146.

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Integrated Schizosaccharomyces pombe (S. pombe) transgenomes in murine cells display a cytological constriction at metaphase. That the metaphase constriction is common to all integrated S. pombe transgenomes suggests that interactions between S. pombe sequences and murine proteins are the cause of these apparently aberrant structures. To investigate which aspects of chromatin packaging within the S. pombe transgenomes are related to this constriction I have studied one of the hybrid cell lines F1.1. I have analysed the amount of S. pombe DNA forming this transgenome and selected regions of it for further investigation. DNA methylation, nucleosome packaging and nucleoskeleton attachment were investigated as potential causes of the constriction. It was found that DNA methylation accumulated within the transgenome during culture, although no correlating alteration in metaphase structure was detected. Methylation was therefore felt not to be related to the observed structure of the transgenome. The transgenome was found to adopt a murine nucleosomal repeat of 185bp, rather than retain its original 160bp S. pombe repeat. Adoption of the host independent of the structure of introduced DNA. This showed that nuclesome not on the underlying DNA sequence. It was possible to demonstrate differential attachment of both murine and S. pombe sequences to the mouse nucleoskeleton. Analysis of and extended region of S. pombe DNA suggests that attachment may by closer, <20kb apart, in the S pombe transgenome, than the 80kb separation expected for mouse DNA. Packaging of these smaller loops at metaphase may lead to the observed constricted structure of the S. pombe transgenomes.

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36

Roberts, Jacqueline Lucy. "A study of replicating instabilities in Schizosaccharomyces pombe". Thesis, University of Edinburgh, 1987. http://hdl.handle.net/1842/14296.

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37

Watts, Beth Rosina. "Investigating mechanisms of transcriptional interference in Schizosaccharomyces pombe". Thesis, University of Oxford, 2015. https://ora.ox.ac.uk/objects/uuid:c919478f-21e9-4061-81aa-4ec1ae41d223.

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Eukaryotic cells transcribe a vast array of non-coding RNAs, most of which have not been assigned a functional role. The work presented here reveals a novel mechanism of transcriptional repression that is mediated by the non-coding RNA prt (pho1-repressing transcript). The prt transcript is shown to recruit a histone deacetylase, Clr3, to repress pho1. This gene encodes a secreted acid phosphatase essential for phosphate acquisition in fission yeast. In the presence of phosphate, prt is produced from an upstream promoter and leads to silencing of pho1. Thus far, this has been explained by prt transcription leading to deposition of repressive methylation over the locus. However, this explanation is known to be incomplete since deletion of the only known histone methyltransferase does not lead to pho1 induction comparable to deletion of the prt promoter. This suggests that another mechanism must be involved in mediating transcriptional interference via non-coding transcription. In the present study the putative ncRNA-binding protein Seb1, together with the chromatin modifying complex SHREC, is demonstrated to associate with prt to elicit silencing of pho1 by a mechanism that is independent of H3K9 methylation and instead relies on deacetylase activity provided by the Clr3 component of SHREC. These data reveal a previously uncharacterised layer of ncRNA-mediated gene regulation and provide important conceptual advances in understanding the mechanisms governing the phenomenon known as transcriptional interference.

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38

Choi, Sang Yong. "Understanding mechanisms of zinc homeostasis in Schizosaccharomyces pombe". The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1420599754.

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39

Takeda, Jun. "Radiation induction of delayed recombination in Schizosaccharomyces pombe". Kyoto University, 2008. http://hdl.handle.net/2433/124231.

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40

Patrick, Andrew. "Novel silencing of alcohol dehydrogenase in Schizosaccharomyces pombe". Connect to resource, 2009. http://hdl.handle.net/1811/37225.

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41

Heyer, Wolf-Dietrich. "Concerted evolution of tRNA genes in Schizosaccharomyces pombe /". [S.l : s.n.], 1985. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.

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42

Silverstein, Rebecca Ann. "Histone deacetylases and their co-regulators in schizosaccharomyces pombe /". Stockholm, 2007. http://diss.kib.ki.se/2007/978-91-7357-140-1/.

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43

Parlati, Francesco. "Characterization of calnexin in Saccharomyces cerevisiae and Schizosaccharomyces pombe". Thesis, McGill University, 1996. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=40421.

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In eukaryotes, the endoplasmic reticulum is the site where folding of secretory proteins and the assembly of multimeric cell surface receptors take place. These processes are mediated by molecule chaperones that include the ER membrane bound chaperone calnexin and the sequence related calreticulin. Using a PCR strategy, a homologue for the mammalian calnexin/calreticulin family, CNE1, was isolated in S. cerevisiae. The CNE1 gene product, Cne!p, is an integral membrane glycoprotein of the ER. Disruption of the CNE1 gene did not lead to inviable cells or to gross effects on the levels of secreted wild type proteins. However, in CNE1 disrupted cells, there was an increase in the cell-surface expression of a normally intracellularly retained temperature sensitive mutant of the $ alpha$-pheromone receptor, Ste2-3p. In addition, an increase in the secretion of heterologously expressed mammalian $ alpha sb1$-antitrypsin was also observed in CNE1 disrupted cells. In order to study calnexin function in another genetically manipulable organism, a Schizosaccharomyces pombe calnexin homologue was sought. Using a similar PCR strategy, a S. pombe calnexin homologue, $cnx1 sp+$, was identified. The $cnx1 sp+$ gene product, Cnx1p, was shown to be a calcium binding type I integral membrane glycoprotein. Unlike the sequence related S. cerevisiae CNE1 gene, the $cnx1 sp+$ gene was essential for cell viability. Full length Cnx1p was able to complement the $cnx1 sp+$ gene disruption but full length mammalian calnexin could not. The ER lumenal domain of Cnx1p, which was secreted from cells, was capable of complementing the $cnx1::ura4 sp+$ lethal phenotype. Both wild type PI M1 (Val 213) $ alpha sb1$-antitrypsin and the ER retained PI Z variant were expressed in S. pombe cells. As in mammalian cells, wild type $ alpha sb1$-antitrypsin was normally secreted whereas the PI Z variant was retained intracellularly. Rescue of the secretion defective phenotype of the PI Z variant occurred in

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44

Pryce, David. "The cloning and characterisation of Schizosaccharomyces pombe rec20-144". Thesis, Bangor University, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.412694.

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45

Glover, James S. A. "Investigation of the ubiquitin proteasome system in Schizosaccharomyces pombe". Thesis, University of Edinburgh, 2010. http://hdl.handle.net/1842/4797.

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Ubiquitin is an essential 76 amino acid protein which can be conjugated to lysine residues on a variety of substrates via its C-terminal diglycine motif. This conjugation allows the protein to act as a molecular tag in a range of processes, including regulation of chromatin compaction, signalling cascades and DNA repair. In addition, ubiquitin moieties are capable of forming chains through the successive conjugation to lysine residues within ubiquitin itself. One of the most well characterized functions of ubiquitin is its role in protein quality control and degradation. Tetra-ubiquitin chains, most commonly through a lysine-48 linkage, are responsible for directing proteins to the 26S proteasome for degradation. This process is of importance both in the removal of miss-folded proteins, and in the regulated destruction of specific targets, such as the cyclins. The 90kDa AAA-ATPase Cdc48/p97/VCP is an essential protein that forms a hexameric complex, which interacts with a wide variety of ubiquitinated substrates. The specificity of Cdc48 is modulated by a series of different cofactors, which together allow Cdc48 to operate in several different contexts, from removal of misfolded proteins from the ER, to regulating securin stability. The role of two Cdc48 cofactors, Ubx4 and Ubx5, was studied in an attempt to dissect their function and to determine how they may modulate the function of Cdc48. Neither protein was found to be essential, as knockouts of either were found to be viable with no major defect in growth rate. The work also describes the findings of a yeast two-hybrid screen to identify potential substrates for both cofactors. Delivery of ubiquitinated proteins to the proteasome is mediated by shuttling factors, which are able to bind to both ubiquitin and the proteasome, and hence mediate the interaction between both. The shuttling factor Dph1 binds ubiquitin via a C-terminal UBA domain, while its N-terminal UBL domain mediates its interaction with the proteasome. This work identified a novel interaction between the Sti1 domains of Dph1 and the N-terminal region of a mitochondrial localized AAA-ATPase, homologous to the Saccaromyces cerevisiae protein Msp1. In addition, cell fractionation experiments revealed the presence of Dph1 at the mitochondria. This interaction provides hints that Mlp1 may be involved in the removal of ubiquitinated proteins from the mitochondria, and their delivery to the proteasome. The thesis begins to try and attempt to identify possible substrates of this proposed mitochondria associated degradation pathway, and looks for ways in which the hypothesis may be tested.

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46

Griffiths, Dominic John Finbar. "Analysis of DNA structure dependent checkpoints in Schizosaccharomyces pombe". Thesis, University of Sussex, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294415.

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47

Lourie, Josephine Anna. "Regulation of the small GTPase ras1p in Schizosaccharomyces pombe". Thesis, Institute of Cancer Research (University Of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312913.

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48

Williams, Emma Samantha. "Characterisation of the schizosaccharomyces pombe MSI1-like protein Prw1". Thesis, University of Newcastle Upon Tyne, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.443990.

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49

Goddard, Alan David. "Functional analysis of GPCR signalling cascades in Schizosaccharomyces pombe". Thesis, University of Warwick, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.437696.

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50

Smith, Matthew William. "Studies of cyclins in the fission yeast Schizosaccharomyces pombe". Thesis, University College London (University of London), 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.266483.

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