Tesi: "Saccharomyces cerevisiae"

1

Schorling, Stefan. "Ceramidsynthese in Saccharomyces cerevisiae". Diss., lmu, 2001. http://nbn-resolving.de/urn:nbn:de:bvb:19-3658.

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2

Deans, Karen. "Ageing of Saccharomyces cerevisiae". Thesis, Heriot-Watt University, 1997. http://hdl.handle.net/10399/663.

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3

Ericson, Elke. "High-resolution phenomics to decode : yeast stress physiology /". Göteborg : Göteborg University, Dept. of Cell and Molecular Biology, Faculty of Science, 2006. http://www.loc.gov/catdir/toc/fy0707/2006436807.html.

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4

Eriksson, Peter. "Identification of the two GPD isogenes of saccharomyces cerevisiae and characterization of their response to hyper-osmotic stress". Göteborg : Chalmers Reproservice, 1996. http://catalog.hathitrust.org/api/volumes/oclc/38202006.html.

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5

Pratt, Elizabeth Stratton. "Genetic and biochemical studies of Adr6, a component of the SWI/SNF chromatin remodeling complex /". Thesis, Connect to this title online; UW restricted, 2001. http://hdl.handle.net/1773/10288.

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6

Kerkmann, Katja. "Die genomweite Expressionsanalyse von Deletionsmutanten der Gene NHP6A/B und CDC73 in der Hefe S.cerevisiae". [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=961961651.

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7

Bellahn, Inga. "Biochemische Charakterisierung vakuolärer Vesikel aus Saccharomyces cerevisiae". [S.l. : s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=965643484.

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8

Jestel, Anja. "Strukturelle Charakterisierung des Calpastatin und Untersuchung eines ATP-abhängigen Peptidtransports in S. cerevisiae". [S.l. : s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=966507193.

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9

Schauen, Matthias. "Mitochondriale Transportproteine in Saccharomyces cerevisiae". [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=965029379.

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10

Schulze, Ulrik. "Anaerobic physiology of Saccharomyces cerevisiae /". Online version, 1995. http://bibpurl.oclc.org/web/20903.

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11

Greig, Duncan. "Sex, species and Saccharomyces cerevisiae". Thesis, University of Oxford, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301401.

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12

Payne, Thomas. "Protein secretion in Saccharomyces cerevisiae". Thesis, University of Nottingham, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.438772.

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13

Paulo, Jorge Fernando Ferreira de Sousa. "mRNA mistranslation in Saccharomyces cerevisiae". Master's thesis, Universidade de Aveiro, 2012. http://hdl.handle.net/10773/7775.

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Mestrado em Biologia Molecular e Celular
The genetic code is defined as a series of biochemical reactions that establish the cellular rules that translate DNA into protein information. It was established more than 3.5 billion years ago and it is one of the most conserved features of life. Over the years, several alterations to the standard genetic code and codon ambiguities have been discovered in both prokaryotes and eukaryotes, suggesting that the genetic code is flexible. However, the molecular mechanisms of evolution of the standard genetic code and the cellular role(s) of codon ambiguity are not understood. In this thesis we have engineered codon ambiguity in the eukaryotic model Sacharomyces cerevisiae to clarify its cellular consequences. As expected, such ambiguity had a strong negative impact on growth rate, viability and protein aggregation, indicating that it affects fitness negatively. However, it also created important selective advantages in certain environmental conditions, suggesting that it has the capacity to increase adaptation potential under environmental variable conditions. The overall negative impact of genetic code ambiguity on protein aggregation and cell viability, suggest that codon ambiguity may have catastrophic consequences in multicellular organisms. In particular in tissues with low cell turnover rate, namely in the brain. This hypothesis is supported by the recent discovery of a mutation in the mouse alanyl-tRNA synthetase which creates ambiguity at alanine codons and results in rapid loss of Purking neurons, neurodegeneration and premature death. Therefore, genetic code ambiguity can have both, negative or positive outcomes, depending on cell type and environmental conditions.
O código genético pode ser definido como uma série de reacções bioquímicas que estabelecem as regras pelas quais as sequências nucleotídicas do material genético são traduzidas em proteínas. Apresenta um elevado grau de conservação e estima-se que tenha tido a sua origem há mais de 3.5 mil milhões de anos. Ao longo dos últimos anos foram identificadas várias alterações ao código genético em procariotas e eucariotas e foram identificados codões ambíguos, sugerindo que o código genético é flexível. Contudo, os mecanismos de evolução das alterações ao código genético são mal conhecidos e a função da ambiguidade de codões é totalmente desconhecida. Nesta tese criámos codões ambíguos no organismo modelo Saccharomyces cerevisiae e estudámos os fenótipos resultantes de tal ambiguidade. Os resultados mostram que, tal como seria expectável, a ambiguidade do código genético afecta negativamente o crescimento, viabilidade celular e induz a produção de agregados proteicos em S. cerevisiae. Contudo, tal ambiguidade também resultou em variabilidade fenótipica, sendo alguns dos fenótipos vantajosos em determinados condições ambientais. Ou seja, os nossos dados mostram que a ambiguidade do código genético afecta negativamente a capacidade competitiva de S. cerevisiae em meio rico em nutrientes, mas aumenta a sua capacidade adaptativa em condições ambientais variáveis. Os efeitos negativos da ambiguidade do código genético, nomeadamente a agregação de proteínas, sugerem que tal ambiguidade poderá ser catastrófica em organismos multicelulares em que a taxa de renovação celular é baixa. Esta hipótese é suportada pela recente descoberta de uma mutação na alaniltRNA sintetase do ratinho que induz ambiguidade em codões de alanina e resulta numa forte perda de neurónios de Purkinge, neurodegeneração e morte prematura. Ou seja, a ambiguidade do código genético pode ter consequências negativas ou positivas dependendo do tipo de células e das condições ambientais.

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14

Kim, Jae-hyun. "Chromosome segregation in Saccharomyces cerevisiae /". Digital version accessible at:, 1998. http://wwwlib.umi.com/cr/utexas/main.

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15

Caponigro, Giordano Michael. "mRNA decay in Saccharomyces cerevisiae". Diss., The University of Arizona, 1996. http://hdl.handle.net/10150/187472.

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mRNA decay is an important step in the control of gene expression. To study mRNA degradation I have exploited the genetic, biochemical, and molecular tools available in Saccharomyces cerevisiae. These studies provided insight into the signals within individual transcripts which specify their half-lives, the various mechanisms by which mRNAs are degraded, and the trans-acting factors which both perform and control nucleolytic events. I identified a 65 nucleotide segment from the coding region of the unstable MATɑl mRNA which was capable of targeting both the MATɑl and stable PGKI transcripts for rapid degradation. This "instability element" was divided into two parts, one located in the first 33, and the second in the latter 32, nucleotides. The first part could be functionally replaced by different mRNA sequences containing rare codons, and while unable to promote mRNA decay by itself, enhanced degradation mediated by the second part. I determined that the MATɑl Instability Element (MIE) targets mRNAs for rapid degradation by increasing the rates of two nucleolytic steps in a pathway of mRNA decay common to several stable and unstable yeast transcripts. The initial step in this pathway is shortening of the poly(A) tail of an mRNA. Subsequently, mRNAs are decapped, after which the transcript body is degraded in a 5' to 3' exonucleolytic manner. The MIE promotes decay of the MATɑl mRNA through an increase in its decapping rate. In contrast, PGKI mRNA decay was stimulated through an increase in its rate of deadenylation. The observation that the poly(A) tail must be removed prior to mRNA decapping suggests that the poly(A) tail inhibits decapping. I determined that the major poly(A)binding protein (Pablp) is required for the inhibition of decapping mediated by the poly(A) tail. Pablp is also required for normal deadenylation rates. Pablp therefore affects mRNA decapping and deadenylation, the two rate determining steps in a common pathway of mRNA decay. Determining how Pablp, and additional trans-acting factors, exert influence over both decapping and deadenylation will provide a greater understanding of the basis of differential rates of mRNA degradation.

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16

Dunckley, Travis Lee. "mRNA decapping in Saccharomyces cerevisiae". Diss., The University of Arizona, 2000. http://hdl.handle.net/10150/289165.

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The major pathway of mRNA degradation in yeast occurs through deadenylation, decapping and subsequent 5' to 3' exonucleolytic decay of the transcript body. The products of the DCP1 and DCP2 genes are required for mRNA decapping. DCP1 encodes a conserved mRNA decapping enzyme. Dcp2p is a highly conserved protein that is required for the activation of Dcp1p. The Dcp2p contains a functional Muff motif that is required for its decapping function, suggesting that Dcp2p encodes a pyrophosphatase. These results suggest that Dcp2p hydrolyzes a specific pyrophosphate bond that either directly activates Dcp1p or removes a specific inhibitor of Dcp1p. In addition to Dcp2p, several additional proteins were identified that influence mRNA decapping. Edc1p and Edc2p are related proteins whose overexpression suppressed conditional mutations in dcp1 and dcp2, respectively. The Edc1 protein interacts in vivo with Dcp1p and Dcp2p. Based on similar genetic data for EDC1 and EDC2, the Edc2p also likely interacts directly with the mRNA decapping machinery. Edc1p and Edc2p may function to activate transitions in the decapping complex that lead to the Dcp2p-dependent activation of Dcp1p. The SBP1 protein was identified as an overexpression suppressor of a conditional dcp2 allele, termed dcp2-7. SBP1 overexpression also suppressed a conditional allele of the decapping enzyme (dcp1-2). In addition, the sbp1Delta was found to partially suppress the decapping defect of the dcp2-7 allele. This suggests that SBP1, which is a highly conserved RNA binding protein related to nucleolin, may influence the assembly or organization of the mRNP. Lastly, loss of function mutations in the previously uncharacterized IDC1 gene were shown to stimulate decapping in the presence of the dcp2-7 mutation. This suggests that the wild-type Idc1p inhibits mRNA decapping. Interestingly, the idc1 mutations described here represent the only known loss of function mRNA decapping suppressors that are not known to influence the rate of translation initiation, suggesting a more direct role for Idc1p in the inhibition of Dcp2p function. Combined, these results indicate that mRNA decapping is a highly controlled process involving the intricate and coordinated function of multiple proteins, in addition to the Dcp1p decapping enzyme.

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17

Anderlund, Mikael. "Redox balancing in recombinant strains of Saccharomyces cerevisiae". Lund : University of Lund, 1998. http://books.google.com/books?id=uc5qAAAAMAAJ.

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18

Ansell, Ricky. "Redox and osmoregulation in Saccharomyces cerevisiae the role of the two isogenes encoding NAD-dependent glycerol 3-phosphate dehydrogenase /". Göteborg : [Institute of Cell and Molecular Biology, Dept. of General and Marine Microbiology, Lundberg Laboratory, Göteborg University], 1997. http://catalog.hathitrust.org/api/volumes/oclc/38985539.html.

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19

Kemp, Hilary A. "A complex of six FAR proteins required for pheromone arrest and mating /". view abstract or download file of text, 2003. http://wwwlib.umi.com/cr/uoregon/fullcit?p3113011.

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Thesis (Ph. D.)--University of Oregon, 2003.
Typescript. Includes vita and abstract. Includes bibliographical references (leaves 94-104). Also available for download via the World Wide Web; free to University of Oregon users.

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20

Strässle, Christoph A. "Modell zur Spontansynchronisation von Saccharomyces cerevisiae /". [S.l.] : [s.n.], 1988. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=8598.

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21

Deckers, Markus. "Charakterisierung peroxisomaler Proteine aus Saccharomyces cerevisiae". [S.l.] : [s.n.], 2007. http://deposit.ddb.de/cgi-bin/dokserv?idn=985178043.

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22

Stüer, Heike. "Wahrnehmung von Biotinmangel durch Saccharomyces cerevisiae". kostenfrei, 2009. http://www.opus-bayern.de/uni-regensburg/volltexte/2009/1353/.

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23

Großmann, Guido. "Plasma membrane compartmentation in Saccharomyces cerevisiae". kostenfrei, 2008. http://www.opus-bayern.de/uni-regensburg/volltexte/2009/1152/.

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24

London, Markus Konrad Justin. "Regulation der Proteasombiogenese in Saccharomyces cerevisiae". [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=974673315.

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25

Beck, Karsten. "Das Dhh1 Protein aus Saccharomyces cerevisiae". Diss., lmu, 2002. http://nbn-resolving.de/urn:nbn:de:bvb:19-7362.

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26

Widlund, Per Olov Ingvar. "The Saccharomyces cerevisiae chromosomal passenger, Bir1 /". Thesis, Connect to this title online; UW restricted, 2006. http://hdl.handle.net/1773/9202.

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27

Thompson, C. L. "Interaction of pentamidine with Saccharomyces cerevisiae". Thesis, University of Hull, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.377415.

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28

Reithinger, Johannes. "Membrane Protein Biogenesis in Saccharomyces cerevisiae". Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-95376.

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Membranes are hydrophobic barriers that define the outer boundaries and internal compartments of living cells. Membrane proteins are the gates in these barriers, and they perform vital functions in the highly regulated transport of matter and information across membranes. Membrane proteins destined for the endoplasmic reticulum are targeted either co- or post-translationally to the Sec61 translocon, the major translocation machinery in eukaryotic cells, which allows for lateral partitioning of hydrophobic segments into the lipid bilayer. This thesis aims to acquire insights into the mechanism of membrane protein insertion and the role of different translocon components in targeting, insertion and topogenesis, using the yeast Saccharomyces cerevisiae as a model organism. By measuring the insertion efficiency of a set of model proteins, we studied the sequence requirements for Sec61-mediated insertion of an α-helical transmembrane segment and established a ‘biological hydrophobicity scale’ in yeast, which describes the individual contributions of the 20 amino acids to insertion. Systematic mutagenesis and photo-crosslinking of the Sec61 translocon revealed key residues in the lateral gate that modulate the threshold hydrophobicity for membrane insertion and transmembrane segment orientation. Further, my studies demonstrate that the translocon-associated Sec62 is important not only for post-translational targeting, but also for the insertion and topogenesis of moderately hydrophobic signal anchor proteins and the C-terminal translocation of multi-spanning membrane proteins. Finally, nuclearly encoded mitochondrial membrane proteins were found to evade mis-targeting to the endoplasmic reticulum by containing short C-terminal tails.

At the time of the doctoral defence the following papers were unpublished and had a status as follows: Paper 4: Manuscript; Paper 5: Manuscript

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29

Spalding, A. C. "Host-plasmid interactions in Saccharomyces cerevisiae". Thesis, University of Kent, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.383082.

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30

Jenkins, F. "Development of thermotolerance in Saccharomyces cerevisiae". Thesis, Bucks New University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.234851.

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31

Pearce, Amanda K. "Regulation of glycolysis in Saccharomyces cerevisiae". Thesis, University of Aberdeen, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301297.

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This thesis extends the work of Crimmins (1995) on the control of glycolytic flux in yeast by the enzymes 6-phosphofructo-1-kinase and pyruvate kinase (Pyk1p). This study also examines the influence of Pf1kp and Pyk1p upon yeast resistance to the weak acid preservative, benzoic acid. In Saccharomyces cerevisiae, Pyk1p is encoded by PYK1, and the α and β subunits of Pf1kp are encoded by PFK1 and PFK2, respectively. To test the influence of these genes upon glycolytic control, an isogenic set of S. cerevisiae mutants were utilised in which PYK1, PFK1 and PFK2 expression is dependent on the PGK1 promoter. Increased Pf1k levels had little effect upon rates of glucose utilisation or ethanol production during fermentative growth. However, overexpressing Pyk1p resulted in an increased growth rate and an increase in glycolytic flux. This suggests that Pyk1p, but not Pf1kp, exerts some degree of control over the glycolytic flux under these conditions. The effects of reducing Pf1kp and Pyk1p levels were also studied by placing PYK1, PFK1 and PFK2 under the control of the weak PGK1Δuas promoter. The double Pf1kp mutant showed no significant changes in doubling time, ethanol production or glucose consumption. However, a mutant with a 3-fold reduction ion Pyk1p levels displayed slower growth rates and reduced glycolytic flux. In addition, there was an imbalance in the carbon flow in this mutant, with reductions in ethanol and glycerol production evident, along with increased TCA cycle activity. Hence, while Pf1kp levels did not affect cell physiology significantly under the conditions studied, reduced Pyk1p levels seemed to disturb glycolytic flux and carbon flow. Decreased Pf1kp levels caused an increase in the sensitivity of yeast cells to benzoate, whereas the Pyk1p mutant was not affected. This confirmed that benzoic acid specifically inhibits Pf1kp rather than glycolysis in general.

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32

Hatton, Lee S. "Gluconeogenic gene regulation in Saccharomyces cerevisiae". Thesis, University of Aberdeen, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.387524.

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The yeast FBP1 and PCK1 genes and the gluconeogenic enzymes that they encode, fructose-1,6-bisphosphatase and phosphoenolpyruvate carboxykinase, are subject to multiple levels of regulation by glucose. It has been reported that transcriptional repression of these genes is exceptionally sensitive to glucose, being triggered by glucose concentrations of less than 0.005% (0.25 mM). It was shown here that in addition at transcriptional repression, the FBP1 and PCK1 and mRNAs are destabilised about 2-fold upon addition of the same low levels of glucose. Low levels of the fermentable sugars fructose or sucrose also stimulated this effect but galactose did not. This destabilisation was lost in a triple hxk1, hxk2, glk1 mutant, but was not triggered by addition of 2-deoxyglucose. The data suggests that sugar phosphorylation and further metabolism of glucose is required to trigger this response. Analysis of metabolic mutants showed that mutations in the upper part of the glycolytic pathway abolish the destabilisation of the FBP1 mRNA. Differences were shown to exist between the regulatory pathways that mediate glucose-stimulated mRNA decay and transcriptional repression. Models which might account for the mechanisms by which rapid decay of the gluconeogenic mRNAs is triggered are discussed. A strategy based on gene fusions with the stable PGK1 mRNA was designed in order to map cis-acting regions which influence PCK1 mRNA stability. A fusion mRNA containing the PCK1 mRNA protein coding region was not destabilised upon addition of low levels of glucose. It was therefore suggested that glucose-stimulated mRNA decay might in some way be dependent upon translation initiation via an interaction with the 5'-leader.

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33

Rowley, Neil K. "Studies on the Saccharomyces cerevisiae genome". Thesis, University of Cambridge, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.361615.

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34

Zealey, Gavin Ross. "Plasmid copy number in Saccharomyces cerevisiae". Thesis, University of Bath, 1985. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.333232.

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Studies were made of 2 mum based chimaeric plasmid copy number in Saccharomyces cerevisiae. A plasmid (pAYE56) containing three selectable genes in yeast (yeast LEU2, bacterial CAT and HSV-1 - TK) was constructed to reflect changes in copy number. Yeast transformants could be grown under three selection regimes and plasmid copy number estimated. During selective growth for the LEU2 gene there are about 20 plasmids per cell. This increases to about 100 during selective growth for the TK gene and furthermore the copy number can be controlled by the stringency of selection. Simultaneous selection for the TK and CAT genes may lead to a further increase (160 copies). Two models are proposed to account for these increases. The amplification model proposes plasmid replication without cell growth whilst the selection model suggests that plasmid copy number varies greatly in a population of transformants and cells with a high copy number are selected for growth under the TK/CAT selection conditions. Whilst the mechanism of copy number increase is unclear, an attempt was made to relate the expression of a heterologous gene (Human alpha2-IFN) to gene dosage using the promotion and secretion signals of the alpha-factor gene. Production of intracellular alpha2-IFN was unaffected by copy number whilst secreted material showed a 100 fold increase over a ten fold increase in gene dosage. Attempts were made to isolate plasmid copy number mutants. After mutagenesis (of cells or plasmid) transformants were selected under conditions for simultaneous over-expression of the TK and CAT genes. Mutants capable of growth under these conditions were obtained. In one group the mutant phenotype was lost upon curing but did not return upon retransformation. In a second group a chromosomal mutation was isolated. Plasmid copy number estimates indicated that this was unchanged however. Alternative strategies are discussed for the isolation of mutants.

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35

Gimeno, Carlos Joaquín. "Characterization of Saccharomyces cerevisiae pseudohyphal development". Thesis, Massachusetts Institute of Technology, 1994. http://hdl.handle.net/1721.1/33506.

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36

DuBern, Charlotte Louise. "Molecular characterisation of Saccharomyces cerevisiae Tra1p". Thesis, University of Cambridge, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.620916.

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37

Bhattacharyya, Souryadeep. "Synthetic sensing systems in Saccharomyces cerevisiae". Thesis, Georgia Institute of Technology, 2014. http://hdl.handle.net/1853/54016.

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The yeast Saccharomyces cerevisiae is a major chemical production platform in the biotechnological industry. It is also increasingly being used as a whole cell biosensor. One method of developing such whole cell biosensors in yeast is by exploiting its mating pathway, which is normally induced by secreted pheromones leading to downstream expression of various genes. Functional expression of different recognition elements or receptors and their coupling to the yeast mating pathway can enable sensing of a variety of ligands. In this work, we have engineered a yeast strain to functionally express a heterologous human olfactory receptor gene which can be coupled to the pheromone signaling pathway, allowing yeast to detect medium chain length fatty acids, alcohols and aldehydes for the first time. Functionally expressing heterologous olfactory receptors in yeast is a challenging task because no definitive method exists on how to express such receptors on the yeast cell surface and couple them to the downstream signaling pathway. We explore in this work how the yeast cell can selectively respond to two activating ligands via two different receptors. We also demonstrate in this work that a synthetic transcription factor can substitute for the native transcription factor in the yeast mating pathway. We believe our biosensor will not only have various uses as a versatile sensor but also aid in the design of synthetic genetic circuits.

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38

Ghimire, Jenisha. "Localization of Ime4 in Saccharomyces cerevisiae". ScholarWorks@UNO, 2012. http://scholarworks.uno.edu/honors_theses/12.

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One lesser-known but universal post transcriptional modification carried out in yeast and higher eukaryotes is the methylation of mRNA, as mediated by the Ime4 protein and its orthologs. Ime4 protein is essential for sporulation in yeast cells and for viability of higher eukaryotic cells. The precise locations of the Ime4 protein and the functions of the methylated mRNA are still largely unknown. Whereas Ime4 protein is believed to be exclusively nuclear in higher eukaryotes, we have observed the yeast Ime4 protein in the nucleus, in the cytosol and within cytosolic particles. These observations suggest that Ime4 could be a shuttling RNA binding protein, playing roles in the cytosol as well as the nucleus. As a first step to examining this idea, we tested the hypothesis that the punctuate cytosolic particles formed by Ime4 are P bodies. P bodies are transient aggregates of proteins and RNAs that form as a result of stresses such as glucose deprivation. This experiment was carried out using fluorescence microscopy using Ime4 tagged with GFP (green fluorescent protein) and the known P -body proteins Edc3, tagged with mCherry. We expected that if the proteins thus produced localized in the same place in the yeast cell, we could then deduce that Ime4 is present in P-bodies. We observed that Ime4 and Edc3 did not colocalize in the majority of cells, and thus concluded that the Ime4 granules are not P-bodies. However, our experiments showed instances of Ime4 signals near or around the P-bodies in some cells. Hence, the Ime4-containing aggregates are not likely to be P-bodies but could rather represent a different type of granule.

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39

Chang, Cheng-Fu. "Compaction of chromatin in Saccharomyces cerevisiae". Master's thesis, University of Cape Town, 2006. http://hdl.handle.net/11427/4247.

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Word processed copy.
Includes bibliographical references.
This study investigated the link between the association of the yeast linker histone homologue, Hholp, and the compaction of the yeast genome during stationary phase. The relative gene content of condensed chromatin, fractionated and isolated by sucrose gradient ultracentrifugation from stationary and exponential phase cultures was compared using genome-wide technologies. This study showed that condensed chromatin of stationary phase culture contained an enriched density of genes on all the chromosomes, indicating global compaction of the yeast genome during stationary phase.

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40

Kadowaki, Tatsuhiko. "Nucleocytoplasmic transport ofmRNA in Saccharomyces cerevisiae". Case Western Reserve University School of Graduate Studies / OhioLINK, 1994. http://rave.ohiolink.edu/etdc/view?acc_num=case1057254977.

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41

Rouhier, Matthew Ford. "Characterization of YDR036C From Saccharomyces cerevisiae". Miami University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=miami1319464136.

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42

Nguyen, Tania. "Complex transcription units in Saccharomyces cerevisiae". Thesis, University of Oxford, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.711667.

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43

Bjarre, Jonas. "Luftning i fedbatchodlingar av Saccharomyces cerevisiae". Thesis, KTH, Skolan för bioteknologi (BIO), 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-190743.

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44

Dangelmayr, Claudia Vera. "Untersuchung der Mikroautophagocytose in Saccharomyces cerevisiae". [S.l. : s.n.], 2004. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB11104004.

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45

Wu, Randy. "Chromatin regulatory signatures in Saccharomyces cerevisiae". Diss., Search in ProQuest Dissertations & Theses. UC Only, 2008. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3339209.

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46

Shock, Teresa R. "Understanding signaling specificity in Saccharomyces cerevisiae". Diss., Search in ProQuest Dissertations & Theses. UC Only, 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3352468.

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47

Henstock, Mark Richard. "Stationary phase genes of Saccharomyces cerevisiae". Thesis, University of Bath, 2004. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.425876.

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Stenner, Nigel Francis. "The WHI1 gene of Saccharomyces cerevisiae". Thesis, University of Bath, 1990. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.237874.

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Marinkovic, Zoran. "Self-organization of Saccharomyces cerevisiae colonies". Thesis, Sorbonne Paris Cité, 2017. http://www.theses.fr/2017USPCC260/document.

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Abstract (sommario):

L’environnement naturel des levures est constitué d’une communauté de cellules. Les chercheurs, cependant, préfèrent étudier les levures dans des environnements plus simples et homogènes, comme des cultures en cellule unique ou en population, s’affranchissant ainsi de la complexité de la croissance spatiotemporelle, la différentiation, l’auto-organisation, ainsi que la façon dont ces caractéristiques sont formées et s’entrelacent à travers l’évolution et l’écologie. Nous avons mis en place un dispositif microfluidique multicouches permettant la croissance de colonie de levures dans des environnements dynamiques, spatialement structurés, contrôlés, partant d’une monocouche de levures à une colonie multicouches. La croissance des colonies, dans son ensemble comme à des positions spécifiques, est le résultat de la formation d’un gradient de nutriment au sein de celles-ci - gradient qui trouve son origine dans le différent taux de diffusion des nutriments, des taux d’absorption de ceux-ci par les cellules, ainsi que de leurs concentrations initiales. Lorsqu’un nutriment en quantité limitante (par exemple le glucose ou un acide aminé) est épuisé, à une distance spécifique de la source de nutriments, les cellules au sein de la colonie cessent de croitre. Nous avons été en mesure de moduler cette distance spécifique en variant la concentration initiale de nutriments ainsi que le taux d’absorption des cellules. Les motifs d’expression de gènes de la colonie nous ont donné des informations sur la formation de micro environnements spécifiques ainsi que sur le développement subséquent, la différentiation et l’auto-organisation. Nous avons quantifié les motifs d’expression de sept gènes de transport du glucose (HXT1-7), chacun exprimé spécifiquement suivant la concentration de glucose, ce qui nous a permis de reconstituer la formation de gradients de glucose au sein d’une colonie. En étudiant des gènes spécifiques de la fermentation et de la respiration, nous avons pu observer la différentiation en deux sous-populations. Nous avons de plus cartographié l’expression de gènes impliqués dans différentes parties du métabolisme des glucides, suivi et quantifié la dynamique spatio-temporelle de croissance et d’expression génétique et finalement modélisé la croissance de la colonie ainsi que la formation du gradient de nutriment. Pour la première fois, nous avons observé la croissance, la différentiation et l’auto-organisation des colonies de S. cerevisiae avec une résolution spatio-temporelle jusqu’à maintenant inégalée
The natural environment of yeast is often a community of cells but researchers prefer to study them in simpler homogeneous environments like single cell or bulk liquid cultures, losing insight into complex spatiotemporal growth, differentiation and self-organization and how those features are intertwined and shaped through evolution and ecology. I developed a multi-layered microfluidic device that allows us to grow yeast colonies in spatially controlled dynamically structured changing environments from a monolayer of single yeast cells to a multi-layered colony. Colony growth, as a whole and at specific locations, is a result of the nutrient gradient formation within a colony through interplay of nutrient diffusion rates, nutrient uptake rates by the cells and starting nutrient concentrations. Once a limiting nutrient (e.g. glucose or amino acids) is depleted at a specific distance from the nutrients source the cells within a colony stop to grow. I was able to modulate this specific distance by changing the starting nutrient concentrations and uptake rates of cells. Colony gene expression patterns gave us information on specific micro environments formation and consequential development, differentiation and self-organization. I quantified the patterns of expression of seven glucose transporter genes (HXT1-7), each of them specifically expressed depending on the glucose concentration. This enabled us to reconstruct glucose gradients formation in a colony. I further followed the expression of fermentation and respiration specific genes and observed differentiation between two subpopulations. We also mapped other genes specific for different parts of carbohydrate metabolism, followed and quantified the spatiotemporal dynamics of growth and gene expression, and finally modelled the colony growth and nutrient gradient formation. For the first time, we were able to observe growth, differentiation and self-organization of S. cerevisiae colony with such an unprecedented spatiotemporal resolution

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Polyak, Steven William. "Biotin protein ligase from Saccharomyces cerevisiae /". Title page, table of contents and summary only, 2000. http://web4.library.adelaide.edu.au/theses/09PH/09php781.pdf.

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Tesi sul tema "Saccharomyces cerevisiae"

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