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Letteratura scientifica selezionata sul tema "Riboflavin"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 29 luglio 2024

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Articoli di riviste sul tema "Riboflavin"

Yamanaka, Gaku, Shinji Suzuki, Natsumi Morishita, Mika Takeshita, Kanako Kanou, Tomoko Takamatsu, Shinichiro Morichi et al. "Experimental and Clinical Evidence of the Effectiveness of Riboflavin on Migraines". Nutrients 13, n. 8 (29 luglio 2021): 2612. http://dx.doi.org/10.3390/nu13082612.

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Riboflavin, a water-soluble member of the B-vitamin family, plays a vital role in producing energy in mitochondria and reducing inflammation and oxidative stress. Migraine pathogenesis includes neuroinflammation, oxidative stress, and mitochondrial dysfunction. Therefore, riboflavin is increasingly being recognized for its preventive effects on migraines. However, there is no concrete evidence supporting its use because the link between riboflavin and migraines and the underlying mechanisms remains obscure. This review explored the current experimental and clinical evidence of conditions involved in migraine pathogenesis and discussed the role of riboflavin in inhibiting these conditions. Experimental research has demonstrated elevated levels of various oxidative stress markers and pro-inflammatory cytokines in migraines, and riboflavin’s role in reducing these marker levels. Furthermore, clinical research in migraineurs showed increased marker levels and observed riboflavin’s effectiveness in reducing migraines. These findings suggest that inflammation and oxidative stress are associated with migraine pathogenesis, and riboflavin may have neuroprotective effects through its clinically useful anti-inflammatory and anti-oxidative stress properties. Riboflavin’s safety and efficacy suggests its usefulness in migraine prophylaxis; however, insufficient evidence necessitates further study.

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Purnakarya, Idral. "Defisiensi Riboflavin dan Demensia pada Usia Lanjut". Kesmas: National Public Health Journal 6, n. 3 (1 dicembre 2011): 99. http://dx.doi.org/10.21109/kesmas.v6i3.99.

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Demensia Alzheimer menempati urutan kesembilan penyebab kematian di Amerika Serikat. Demensia adalah kondisi yang sering dialami yang berhubungan dengan berbagai faktor dan gaya hidup terutama diet. Penelitian ini bertujuan untuk mengetahui hubungan defisiensi asupan riboflavin (vitamin B12) dengan demensia pada usia lanjut (usila). Penelitian ini menggunakan desain studi cross sectional dan dilaksanakanpada bulan September 2007 sampai dengan Januari 2008. Sampel penelitian adalah 141 lansia berumur lebih dari sama dengan 60 tahun yang diambil secara purposive sampling. Demensia diukur menggunakan kuesioner MMSE (² 24, skor maksimum 30) dan asupan riboflavin diukur menggunakan form Semi Quantitative – FFQ. Penelitian ini memperlihatkanbahwa 47,5% usila mengalami demensia. Hasil uji statistik menunjukkan bahwa terdapat hubungan yang signifikan antara umur, tingkat pendidikan, dan asupan riboflavin dengan kejadian demensia pada usila (nilai p < 0,05).Kata kunci: Demensia, defisiensi riboflavin, usia lanjutAbstractDementia Alzheimer’s was ranked the ninth leading cause of death in The United States. Dementia can not be avoided as related to several factors and lifestyle especially the diet. The objective of this research is to know relation the deficiency of riboflavine (vitamin B12) intake and incidence of dementiaat elderly. A cross-sectional study was conducted betweenSeptember 2007 and January 2008. The sample obtained was 141 elderly which it was conducted to purposive sampling. Dementia was measured by using questionnaire MMSE (² 24, maximum score was 30), and riboflavine intake was measure by Semi Quantitative – FFQ form. This study shows that dementia in elderly was 47,5%. Statistical test showed that Statistical test showed that incidence of dementia had significantly associated with ages, level of education, and riboflavine intake (p value < 0,05).Key words: Dementia, deficiency of riboflavine, elderl

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Pinto, John T., e Janos Zempleni. "Riboflavin". Advances in Nutrition 7, n. 5 (1 settembre 2016): 973–75. http://dx.doi.org/10.3945/an.116.012716.

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&NA;. "Riboflavin". Reactions Weekly &NA;, n. 1365 (agosto 2011): 40. http://dx.doi.org/10.2165/00128415-201113650-00151.

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&NA;. "Riboflavin". Reactions Weekly &NA;, n. 1411 (luglio 2012): 38–39. http://dx.doi.org/10.2165/00128415-201214110-00139.

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&NA;. "Riboflavin". Reactions Weekly &NA;, n. 893 (marzo 2002): 13. http://dx.doi.org/10.2165/00128415-200208930-00050.

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&NA;. "Riboflavin". Reactions Weekly &NA;, n. 1430 (dicembre 2012): 28. http://dx.doi.org/10.2165/00128415-201214300-00101.

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White, H. B., J. Armstrong e C. C. Whitehead. "Riboflavin-binding protein. Concentration and fractional saturation in chicken eggs as a function of dietary riboflavin". Biochemical Journal 238, n. 3 (15 settembre 1986): 671–75. http://dx.doi.org/10.1042/bj2380671.

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The concentration of riboflavin and riboflavin-binding protein were determined in the plasma, egg yolk and albumen from hens fed a riboflavin-deficient diet (1.2 mg/kg) supplemented with 0, 1, 2, 3, 10 and 40 mg of riboflavin/kg. We observed that the deposition of riboflavin in egg yolk and albumen is dependent on dietary riboflavin and reaches half-maximal values at about 2 mg of supplemental riboflavin/kg. The maximal amount of riboflavin deposited in the yolk is limited stoichiometrically by the amount of riboflavin-binding protein, whereas the maximum amount of riboflavin deposited in albumen is limited by other factors before saturation occurs. The amount of riboflavin-binding protein in yolk and albumen is independent of dietary riboflavin. If there is a specific oocyte receptor for riboflavin-binding protein, it cannot distinguish between the apo and holo forms of the protein. Riboflavin-binding protein is about six times more concentrated in yolk than in plasma.

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Porcelli, Peter J., Mary L. Rosser, Dia DelPaggio, Eugene W. Adcock, Larry Swift e Harry Greene. "Plasma and Urine Riboflavin During Riboflavin‐Free Nutrition in Very‐Low‐Birth‐Weight Infants". Journal of Pediatric Gastroenterology and Nutrition 31, n. 2 (agosto 2000): 142–48. http://dx.doi.org/10.1002/j.1536-4801.2000.tb07079.x.

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ABSTRACTBackgroundVery‐low‐birth‐weight (VLBW; birth weight <1500 g) infants receive enteral and parenteral nutriture that provides greater daily riboflavin (vitamin B2) than does term infant nutriture, and elevated plasma riboflavin develops in these infants after birth. The purpose of this study was to measure plasma and urine riboflavin concentrations in VLBW infants during riboflavin‐free nutrition. Our hypothesis was that elevated plasma riboflavin develops in VLBW infants because of high daily intake and immature renal riboflavin elimination.MethodsEighteen clinically healthy VLBW infants received parenteral nutrition and preterm infant formula during the first postnatal month. On postnatal days 10 and 28, the infants received specially prepared riboflavin‐free enteral and parenteral nutrition for the 24‐hour study period. Serial collections of plasma were made at time 0 and at 12 and 24 hours. Urine was collected continuously for the 24‐hour period in 4‐hour aliquots. Samples were analyzed for riboflavin concentration.ResultsDuring the 24‐hour riboflavin‐free study period on postnatal day 10, plasma riboflavin decreased 56% from 185 ± 37 ng/mL (mean ± SEM), and urine riboflavin decreased 75% from 3112 ± 960 mg/mL. Similarly, on postnatal day 28, plasma riboflavin decreased 79% from 184 ± 32 ng/mL, and urine riboflavin concentration decreased 91% from 5092 ± 743 ng/mL during the 24‐hour riboflavin‐free study period. Riboflavin half‐life (t½) was 18.5 hours on postnatal day 10 and decreased 48% by postnatal day 28. Riboflavin elimination was 145.1 ± 20.6 mg/kg per day on postnatal day 10 and increased 40% by postnatal day 28.ConclusionThe VLBW infants who received parenteral nutrition and preterm infant formula had elevated plasma riboflavin on postnatal days 10 and 28. Plasma riboflavin t½ was shorter and renal riboflavin elimination was greater on postnatal day 28 than on postnatal day 10. Plasma riboflavin was normal after 24 hours of riboflavin‐free nutrition. The pattern of plasma and urine riboflavin in VLBW infants suggests a lower daily intake would maintain plasma riboflavin close to normal.

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Mack, Matthias, e Simon Grill. "Riboflavin analogs and inhibitors of riboflavin biosynthesis". Applied Microbiology and Biotechnology 71, n. 3 (luglio 2006): 265–75. http://dx.doi.org/10.1007/s00253-006-0421-7.

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Più fonti

Tesi sul tema "Riboflavin"

Grippa, Juliana Malvestio. "Reatividade das espécies heme-Fe metmioglobina e oximioglobina frente ao estado singlete e triplete excitado da riboflavina". Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/75/75133/tde-02072014-144920/.

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O pigmento da carne fresca, oximioglobina, e a sua forma oxidada, metmioglobina, podem ser ambos oxidados pela riboflavina quando expostos à radiação luminosa, afetando sua estabilidade redox da carne do ponto de vista nutricional e sensorial. A reação da MbFe(II)O2 e da MbFe(III) com o estado tripleto da riboflavina, 3Rib, envolve uma eficiente transferência de elétrons entre o anel isoaloxazina da riboflavina e a cadeia polipeptídica da proteína, o que leva à formação de cross-link e/ou fragmentação, como demostrado por SDS-PAGE e Western-blot. A constante global de velocidade para a oxidação da MbFe(II)O2 pela 3Rib é (3,0 ± 0,5 ) 109 L·mol-1·s-1 e de (3,1 ± 0,4) 109 L·mol-1·s-1 para a oxidação da MbFe(III) pelo estado tripleto da riboflavina. Cálculos termodinâmicos demonstram ainda que há formação de um complexo exotérmico com estequiometria 1:1 favorecido a temperaturas mais baixas com Ka = (1.2 ± 0.2) 104 mol·L-1 a 25 °C e ΔHo = -112 ± 22 kJ·mol-1 e ΔSo = -296 ± 75 J·mol-1·K-1. Conclui-se que para carne, a riboflavina é um fotossensibilizador para oxidação de proteína e não para a descoloração.
The fresh meat pigment oxymyoglobin, MbFe(II)O2, and its oxidized form metmyoglobin, MbFe(III), are both oxidized by riboflavin as photosensitizer. The reaction of MbFe(II)O2 and MbFe(III) with triplet-state riboflavin, 3Rib, involves the pigment protein, which is oxidatively cleaved or dimerized as shown by SDS-PAGE and Western-blotting, while the heme iron center is not oxidized. The over-all rate constant for oxidation of MbFe(II)O2 by 3Rib is (3.0 ± 0.5) 109 L·mol-1·s-1 and (3.1 ± 0.4) 109 L·mol-1·s-1 for MbFe(III) in aqueous 0.20 mol·L-1 NaCl phosphate buffer of pH 7.4 at 25 °C as determined by transient absorption laser flash photolysis. The high rates are rationalized by ground state hydrophobic interactions as detected as static quenching of fluorescence from singlet-excited state riboflavin by myoglobins using single photon counting time resolved fluorescence spectroscopy and a Stern-Volmer approach. Binding of riboflavin to MbFe(III) has Ka = (1.2 ± 0.2) 104 mol·L-1 at 25 °C with ΔHo = -112 ± 22 kJ·mol-1 and ΔSo = -296 ± 75 J·mol-1·K-1. For meat, riboflavin is concluded to be a photosensitizer for protein oxidation but not for discoloration.

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Subbotina, Beztsinna Nataliia. "Riboflavin-based amphiphiles for tumour-targeted nanosystems". Thesis, Bordeaux, 2015. http://www.theses.fr/2015BORD0254/document.

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La riboflavine (RF) est une vitamine essentielle pour la croissance et le développement cellulaire. Elle possède des propriétés physico-chimiques intéressantes et est internalisée dans les cellules par des transporteurs spécifiques. Le premier objectif de ce projet était de synthétiser des dérivés amphiphiles de la RF (RFA) et d'étudier leurs capacités d'auto-assemblages. Le second objectif était d'insérer les RFA dans des liposomes et d'évaluer leur efficacité de ciblage tumoral in vitro et in vivo. La préparation des différents RFA repose sur l'ajout d'un lipide en différentes positions de la RF. L’un d'eux, de type phospholipide (RfdiC14) a été capable de former des objets tridimensionnels de taille μm constitués de lamelles multicouches dont l’architecture et la dynamique sont très différentes de celles des phospholipides classiques. L’insertion de RfdiC14 dans des liposomes est efficace et n’influence pas leurs propriétés physico-chimiques. Les liposomes fonctionnalisés ont montré une internalisation cellulaire spécifique dans les lignées A431, PC3 et HUVECs. Afin de tester l’efficacité du ciblage tumoral in vivo, un analogue de RfdiC14 portant un espaceur PEG a été préparé puis inséré dans des liposomes péguylés. Grâce à un marquage adéquat (ICG et DiR), leur accumulation tumorale a été suivie par imagerie photoacoustique dans un modèle A431 et leur biodistribution évaluée par imagerie μCT/FMT dans un modèle PC3. Les résultats montrent une légère amélioration de l’accumulation tumorale dans les xénogreffes A431 et une augmentation du ciblage vasculaire dans le modèle tumoral PC3. La biodistribution globale des liposomes marqués est comparable à celle des contrôles
Riboflavin (RF) is an essential vitamin for cell growth and development. It possesses interesting physicochemical properties and is internalized by the cells through specific transporters. The first aim of this study was to prepare amphiphile derivatives of RF (RFA) and study their auto-assembly. The second aim was to insert RFA into established drug delivery systems and test their tumour-targeting potential in vitro and in vivo. RFA were prepared by the molecule functionalization with lipid moieties in different positions. One of them, a phospholipid-like derivative (RfdiC14) was able to self-assembly in aqueous solutions into μm-sized 3D objects constituted from slightly curved multilayer lamellas. The bilayer architecture and dynamics were very different from ordinary phospholipids. In contrast, the insertion of small amount of RfdiC14 in a liposome did not influence membrane dynamics and physicochemical characteristics. RfdiC14-functionalised liposomes displayed high and specific uptake in vitro in A431, PC3 cells and HUVECs. The efficiency of RF targeting was also tested in vivo. For that purpose, liposome composition was optimized and a new RF amphiphile with a PEG spacer between RF and lipid was prepared. The tumour accumulation of the liposomes labelled with ICG was studied by photoacoustic imaging in A431 tumour model. The biodistribution of DiR labelled liposomes was accessed by combined μCT/FMT imaging in PC3 tumour model. The results show slight improvement of the tumour accumulation in A431 xenographts and the enhancement of vascular targeting in PC3 tumour model. The overall biodistribution of the RF-targeted liposomes was comparable to control

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Machado, Daisy 1981. "Modulação da agressividade do melanoma por flavinas". [s.n.], 2012. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314042.

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Orientadores: Carmen Verissima Ferreira Halder, Silvia Mika Shishido
Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
Made available in DSpace on 2018-08-20T22:59:08Z (GMT). No. of bitstreams: 1 Machado_Daisy_D.pdf: 7339714 bytes, checksum: b126414072e5dc8abcca091c7af0fdbf (MD5) Previous issue date: 2012
Resumo: Melanoma é o tipo mais agressivo de tumor de pele e uma das principais causas de morte por tumor de pele, devido a sua alta capacidade metastática. Em termos de estratégias terapêuticas de combate ao melanoma tem-se dado ênfase no controle da resistência e da metástase. Nosso grupo de pesquisa observou que a riboflavina irradiada (RFi) induz apoptose de células de câncer de próstata, renal e leucemia mielóide. Portanto, o objetivo geral deste trabalho foi utilizar a RFi para modular química e geneticamente as vias de transdução de sinal associadas com a sobrevivência, resistência e agressividade do melanoma. Assim, neste trabalho estão apresentados os dados sobre a influência da RFi em diferentes aspectos metabólicos das células de melanoma murino (B16F10), tais como: citotoxicidade, adesão, invasão, migração, capacidade de formação de colônia e em mediadores de transdução de sinal: Src quinase, mTOR e componentes da via sonic hedgehog. Em todos os experimentos a riboflavina (RF) foi previamente irradiada com UVA (dose de 9 J/cm²). Foi observado inibição da proliferação celular com valor de IC50 de 50 ?M. De forma interessante, RFi na faixa de concentração de nanomolar foi eficiente na inibição da formação de colônias. Além disso, causou a redução da adesão das células B16F10, quando utilizada na concentração de 1?M. A capacidade de migração e invasão das células de melanoma foi reduzida na presença da RFi, nas concentrações de 1 e 30 ?M respectivamente, porém a resposta foi independente da dose. A atividade e expressão das metaloproteinases foram diminuídas na presença de RFi, indicando inibição na capacidade de invasão. Sob o contexto de sinalização a RFi modulou negativamente a via sonic hedgehog, PI3K/mTOR e aumentou a expressão da p53 e PTEN. O conjunto de resultados obtidos nesse trabalho mostra que flavinas são candidatas promissoras para a intervenção farmacológica do melanoma
Abstract: Melanoma is the most aggressive type of skin disorder and a major cause of death by skin's disease due to its highly metastatic ability. In terms of melanoma therapeutic strategies has given emphasis on control of resistance and metastasis. Our group observed that irradiated riboflavin (RF) induces apoptosis of prostate cancer cells, kidney cancer cells and myeloid leukemia. Therefore, the goal of this study was to employ irradiated RF for modulating chemical and genetically signal pathways associated with melanoma survival, resistance and aggressiveness. Thus, in this manuscript data about the influence of RF in different cellular metabolic aspects of murine melanoma (B16F10) such as cytotoxicity, adhesion, invasion, migration, colony formation and signal transduction mediators Src kinase, mTOR and sonic hedgehog components, will be presented. In all experiments the RF was previously irradiated with UVA (dose of 9 J/cm²). Inhibition of cell proliferation was observed with IC50 value of 50 ?M. Interestingly, RF in a nanomolar concentration inhibited the formation of colonies. In addition, 1 ?M irradiated RF caused a reduction of B16F10 cells adhesion. The ability of migration and invasion of melanoma cells was reduced in the presence of RF, however, those cells response was dose-independent. The activity and expression of metalloproteinases were diminished indicating reduction of cellular invasiveness capacity. Sonic hedgehog and PI3K/mTOR pathways were negatively modulated and the expression of p53 and PTEN were increased in melanoma cells treated with irradiated RF. The findings showed in this study brought out flavins as promising candidates for pharmacological intervention of melanoma
Doutorado
Bioquimica
Doutor em Biologia Funcional e Molecular

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Ohara, Andre 1989. "Isolamento e seleção de leveduras silvestres de biomas do Estado de São Paulo com potencial para produção de lipase e vitaminas do complexo B". [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/256642.

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Orientador: Gabriela Alves Macedo
Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
Made available in DSpace on 2018-08-24T18:55:36Z (GMT). No. of bitstreams: 1 Ohara_Andre_M.pdf: 6935792 bytes, checksum: 553cf8492d7af096c35b5c595ec6e3a1 (MD5) Previous issue date: 2014
Resumo: A bioprospecção de micro-organismos representa um grande potencial tecnológico para produção de biocompostos de interesse comercial. Dentre estes compostos estão as enzimas lipolíticas, como as lipases (triacilglicerol acil-hidrolases EC 3.1.1.3) que apresentam um enorme potencial para aplicações biotecnológicas. Outro tipo de biocomposto de relevante interesse comercial são as vitaminas do complexo B, as quais possuem função essencial na atividade de enzimas que regulam reações do metabolismo. Desta forma, o objetivo desse trabalho foi isolar e selecionar linhagens de leveduras do solo de diferentes biomas do Estado de São Paulo com potencial para produção de lipase e de vitaminas do complexo B (biotina e riboflavina). Para tanto, foram isoladas 132 leveduras de amostras de solos provenientes da Mata Atlântica e da região de transição (Mata Atlântica e Cerrado). Essas linhagens foram depositadas na coleção de micro-organismos do laboratório de Bioquímica de Alimentos da FEA-UNICAMP, que já contava com 300 leveduras provenientes da região de Cerrado. As 432 linhagens presentes na coleção foram então avaliadas quanto ao potencial para a produção de lipase extracelular através de seleção em meio sólido diferencial e cultivo em meio líquido, sendo a atividade de lipase determinada no sobrenadante por titulometria de neutralização. O potencial para a produção de biotina e riboflavina extracelular foi avaliada por meio de seleção em meio de cultivo líquido, sendo as concentrações das vitaminas determinadas no sobrenadante por espectrofotometria e fluorimetria. Desta forma 33 leveduras apresentaram potencial para produção de lipase alcançando valores de atividade enzimática que variaram de 6,51 U/mL a 21,44 U/mL. Em relação à produção das vitaminas do complexo B, 38 linhagens apresentaram concentrações de biotina no sobrenadante do cultivo que variaram de 0,28 µg/mL a 18,61 µg/mL e 64 linhagens apresentaram concentrações de riboflavina que variaram de 0,03 µg/mL a 0,77 µg/mL. A linhagem RP.C153 apresentou potencial para produção de lipase e riboflavina, enquanto a linhagem RP.J1308 para produção de lipase e biotina. A linhagem RP.C153 foi classificada como Pichia caribbica e a linhagem RP.J1308 como Candida oleophila
Abstract: The bioprospection of microorganisms represents a great technological potential for the production of biocompounds of commercial interest. Among these compounds are the lipolytic enzymes, such as lipases (triacilglicerol acilhidrolases EC 3.1.1.3) which have currently a huge potential for biotechnological applications. B vitamins are another type of biocompound with relevant commercial interest, which have essential role in the activity of enzymes that regulate metabolic reactions in living organisms. Therefore, the aim of this work was to isolate and select strains of yeasts from the soil of different biomes present in the State of São Paulo for the biotechnological production of lipase and B vitamins (biotin and riboflavin). Thus, 132 yeasts were isolated from soil samples of the Atlantic Forest (Ilha Bela - SP) and the transition region between the Atlantic Forest and Savanna (Campinas - SP). These strains were deposited in the collection of microorganisms of the Food Biochemistry Laboratory FEA - UNICAMP, which already had 300 yeasts from the Savanna region (Ribeirão Preto - SP). Therefore 432 strains present in the collection were then evaluated for their potential to produce extracellular lipase by selection on solid selective differential and liquid medium culture, and the enzyme activity in the supernatant was determined by neutralization titration. The potential production of extracellular biotin and riboflavin was assessed by selection in medium liquid culture, and the concentrations of those vitamins were measured in the supernatant by spectrophotometry and fluorimetry. Therefore 33 yeasts demonstrated potential for the production of lipase, reaching values of enzymatic activity ranging from 6.51 U/mL to 21.44 U/mL. In relation to the production of B vitamins, 38 strains presented concentrations of biotin in the supernatant culture ranging from 0.28 µg/mL to 18.61 µg/mL and 64 strains demonstrated concentrations of riboflavin ranging from 0.03 µg/mL to 0.77 µg/mL. The strain RP.C153 presented potential for production of lipase and riboflavin, while strain RP.J1308 for production of lipase and biotin. The RP.C153 strain was classified as Pichia caribbica and RP.J1308 strain as Candida oleophila
Mestrado
Ciência de Alimentos
Mestre em Ciência de Alimentos

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Øyangen, Julia. "Photoprotection of riboflavin containing beverages". Thesis, Norges teknisk-naturvitenskapelige universitet, Institutt for fysikk, 2012. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-18386.

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Riboflavin, also known as vitamin B2 and one of the most easily absorbed nutrients,can be found in many different organisms. The most abundant source of riboflavin ismilk and dairy products; however it is also present in meat, fish and certain types ofvegetables and fruit. Riboflavin is an important part of a healthy diet in order to keep skin, eyes and nervous systems healthy. Some studies indicate that riboflavin plays an important role in cancer and cardiovascular diseases.As known, milk is extremely sensitive to light. Riboflavin is one of the factorsresponsible for the light-induced degradation of milk. In combination with light andoxygen riboflavin may act as a photosensitizer. When vitamin B2 absorbs blue-greenlight, an excited triplet state of riboflavin is generated through a process called intersystem crossing. Reactive oxygen species, such as singlet oxygen, is then formed by reaction of excited riboflavin triplet with dissolved oxygen present in milk. Light exposure of milk can lead to off-flavor and damage of vitamins by reaction of singlet oxygen with amino acids and lipids in milk. Unfortunately, most of the packaging materials today do not protect milk from light completely. The formation of singlet oxygen can also be prevented by adding quenchers that are able to deactivate riboflavin triplets.Certain amino acids and carotenoids are well known flavin quenchers.The purpose of this study was to investigate how well riboflavin triplets can bequenched by amino acids cysteine, histidine, methionine, tyrosine and tryptophan. Thequenching properties of hydrophilic carotenoid crocin were studied as well. Crocinhas been under investigation of researches at the Departement of Physics at NTNU.Lumiflavin, which is one of the riboflavin’s photodegradation products, was used instead of riboflavin. The former is more stable and has similar photochemical characteristics as the latter.The quenching of lumiflavin triplets was studied by using laser flash photolysis. Itconsists of irradiating the sample under investigation with a short-lived laser flash. The method was used to measure the kinetic decay rate of lumiflavin in aqueous buffer with and without different concentrations of a quencher. The data were fitted to two different decay models. From pseudo-first-order rate constants the quenching rate constants were determined for each amino acid and crocin. All amino acids and crocin used in this study showed a quenching effect on the lumiflavin triplets. Further, it was determined whether the fitting models are suitable for these kind of measurements by simulating the decay of lumiflavin with and without any quencher. More studies on the fitting models have to be done to be able to get reliable results.

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Yates, Catherine Ann. "Gastrointestinal development and riboflavin deficiency". Thesis, University of Sheffield, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312293.

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Huang, Se-Ne. "Cellular translocation mechanism of Riboflavin /". The Ohio State University, 2001. http://rave.ohiolink.edu/etdc/view?acc_num=osu1486572165275525.

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Riether, Gustavo Tokoro. "Aspectos do mecanismo de formação 3-metil-2-buteno-1-tiol em cerveja e a reatividade dos iso-α-ácidos". Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/75/75132/tde-21092010-091119/.

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A cerveja é uma bebida alcoólica fermentada derivada do amido e aromatizada com lúpulo (Humulus lupulus L.). Os α-ácidos são extraídos do cone do lúpulo e durante o processo de cozimento do mosto são isomerizados em iso-α-ácidos (IAAs), na configuração cis- e trans-, conferindo qualidade de espuma e sabor amargo característico da cerveja. Neste trabalho, é reportado que IAAs sofrem degradação fotossensibilizada por flavinas (Φ = 4,8x10-3 mol einstein-1), mesmo na presença de compostos fenólicos (ácido ferúlico, Φ = 2,0x10-3 mol einstein-1) em excesso molar de 10 vezes, sugerindo que radicais formados pela desativação do estado tripleto excitado da riboflavina por compostos fenólicos possam também estar envolvidos na degradação dos IAAs. Foram identificados dímeros e trímeros derivados do ácido ferúlico e p-coumárico através de LC-ESI-IT-MS como principais fotoprodutos de degradação dos compostos fenólicos. Reportamos a reatividade dos diferentes diastereoisômeros de iso-α-ácidos frente ao radical 2,2-difenil-1-picrilhidrazila (DPPH•), como modelo de radical peroxila, k2 = 0,41 e 1,3 L mol-1 s-1 para a reação com cis-IAA e trans-IAA em meio de etanol acidificado com 1 % ácido fórmico a temperatura de 25 °C, respectivamente. Estas constantes de velocidade específica sugerem que a degradação dos ácidos amargos via reação térmica em processo radicalar é importante no armazenamento do produto já que as constantes de velocidade de reação dos IAAs com o radical DPPH• são competitivas com as observadas para as reações de antioxidantes naturalmente presentes na cerveja com o radical DPPH• ([ácido ferúlico] = 0,2mg/Lcerveja; k2 = 1,18.102 M-1s-1). A análise dos dados termodinâmicos (Mistura de IAAs, ΔH‡ = 25 kJ mol-1 e ΔS‡ = -155 J mol-1 K-1) sugere um mecanismo de oxidação dos IAAs pelo radical DPPH• via HAT/PCET. A diferença de reatividade observada para os diastereoisômeros (cis/trans) está aparentemente relacionada ao arranjo estereoquímico dos grupos laterais isohexonoil e prenil conectados aos carbonos C(4) e C(5), respectivamente. Desta forma, sugere-se que a proximidade espacial dos sítios de insaturação na espécie trans- ocasiona um aumento na densidade eletrônica ou um fator apenas estatístico já que os H-alílicos estão próximos espacialmente, favorecendo desta forma a oxidação via radicalóide.
Beer is a fermented alcoholic beverage based on starch and flavored by hops (Humulus lupulus L.). The α-acids are extracted from hop cones and isomerize into iso-α-acids (IAAs) during the wort boiling, in cis- and trans- configuration, providing foam quality and the characteristic bitter taste of beer. In this work, is reported that these compounds undergo degradation photosensitized by flavins (Φ = 4,8x10-3 mol einstein-1), even in the presence of phenolic compounds (ferulic acid, Φ = 2,0x10-3 mol einstein-1) in 10-fold molar excess, suggesting that radicals formed during the deactivation of triplet excited state of riboflavin by phenolic compounds may be involved in the degradation of IAAs. Dimers and trimers derived from ferulic and p-coumaric acids were identified by LC-ESI-IT-MS as the main photoproducts of the phenolic compounds. We report the reactivity of the different diastereoisomers of IAAs towards the 2,2-diphenyl-1-picrylhydrazyl (DPPH•) radical, as a model for peroxyl radical, k2 = 0,41 e 1,3 L mol-1 s-1 for the reaction with cis-IAA and trans-IAA in ethanol acidified with 1% of formic acid, at the temperature of 25 °C, respectively. These specifics rate constants suggest that the degradation of the bitter acids via thermal reactions in an radicaloid process is important during the storage of the product since the reaction rate constant for IAAs and the DPPH• radical are competitive with the reaction rate constants for naturally occurring antioxidants in beer with the DPPH• radical ([ferulic acid] = 0,2mg/Lbeer; k2 = 1,18.102 M-1s-1). The analysis of the thermodynamical data (IAAs mixture, ΔH‡ = 25 kJ mol-1 e ΔS‡ = -155 J mol-1 K-1) suggest a HAT/PCET oxidation mechanism of IAAs by DPPH• radical. The difference of reactivity observed for the diastereoisomers (cis-/trans-) is possibly related to the stereochemical arrangement of the isohexonoyl and prenyl side chains connected to C(4) and C(5) carbons, respectively. In this way, is suggested that the spatial proximity of the insaturation sites in the trans- species lead to a increase in electronic density or due to a statistical factor since the allylic-H are close spatially, which favors the oxidation via radicaloid.

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Munive, Mendez María Claudia del Pilar, e Quispe Flavia Jimena Cardenas. "Evaluación in vitro del efecto inhibitorio de la terapia fotodinámica sobre Streptococcus mutans (ATCC® 25175) y Streptococcus sanguinis (ATCC® 10556) en presencia y ausencia de riboflavina". Bachelor's thesis, Universidad Peruana de Ciencias Aplicadas (UPC), 2020. http://hdl.handle.net/10757/651668.

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Objetivo: Evaluar el efecto inhibitorio de la terapia fotodinámica (TPD) con Diodo Emisor de Luz (LED) azul sobre Streptococcus mutans y Streptococcus sanguinis en presencia y ausencia de riboflavina (E - 101). Materiales y métodos: Se realizaron cuatro tratamientos en presencia y ausencia de la exposición de luz LED azul y riboflavina al 0.5% sobre Streptococcus mutans y Streptococcus sanguinis. Las bacterias fueron cultivadas en medio BHI y la unidad de medida utilizada fue las unidades formadoras de colonias (UFC/ml). Resultados: La fotoactivación con luz LED azul a 40 segundos no tuvo efecto inhibitorio sobre S. mutans y S. sanguinis. Sin embargo, al realizar la terapia fotodinámica en presencia de riboflavina, se observó que el crecimiento bacteriano fue menor (p<0.05). Asimismo, se identificó que la viabilidad bacteriana de S. sanguinis es menor que la de S. mutans, con un 40% y 66% respectivamente. Conclusiones: Se concluye que la riboflavina tiene un efecto inhibitorio significativo sobre la viabilidad bacteriana de S. mutans y S. sanguinis.
Objective: To evaluate the inhibitory effect of photodynamic therapy (TPD) with blue Light Emitting Diode (LED) on Streptococcus mutans and Streptococcus sanguinis in presence and absence of riboflavin (E-101). Materials and methods: Four treatments were performed in presence and absence of blue LED and riboflavin (0.5%) exposure on Streptococcus mutans and Streptococcus sanguinis. The bacteria were grown in BHI medium and the unit of measurement used was the colony forming units (CFU / ml). Results: Photoactivation with blue LED light at 40 seconds had no inhibitory effect on S. mutans and S. sanguinis. However, when performing photodynamic therapy in presence of riboflavin, it was observed that bacterial growth was lower (p <0.05). Likewise, it was identified that bacterial viability of S. sanguinis is lower than S. mutans, with 40% and 66% respectively. Conclusions: It is concluded that riboflavin has a significant inhibitory effect on the bacterial viability of S. mutans and S. sanguinis.
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McClelland, David Andrew. "The refolding of riboflavin binding protein". Thesis, University of Stirling, 1996. http://hdl.handle.net/1893/3408.

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Hen egg riboflavin binding protein (RfBP) acts as a source of riboflavin to the developing embryo. It is the most abundant vitamin binding protein in the egg white. Mutations giving rise to a lack of RfBP lead to embryo death at approximately 13 days. RfBP binds riboflavin tightly in a 1:1 ratio. On formation of this complex, the fluorescence of riboflavin is completely quenched; this quenching is thought to be due to the stacking of aromatic groups within the hydrophobic binding pocket. This quenching provides a convenient assay for the integrity of the riboflavin-binding site of the protein. RfBP consists of a single polypeptide chain of 219 amino acids of molecular mass 29.2 kDa. RfBP undergoes a number of post-translational modifications, namely: the formation of nine disulphide bonds, extensive glycosylation on Asn 36 and Asn 147, and the phosphorylation of eight serine side chains from between Ser 186 and Ser 197. The unfolding and refolding of RfBP was studied by denaturing in 6M guanidium chloride, followed by dilution in buffer, to start refolding. The processes were followed by both steady-state and stopped-flow circular dichroism and fluorescence spectroscopy. RfBP was found to readily unfold and refold, provided the disulphide bonds were intact. The regain of secondary structure was found to be too rapid to measure by the methods available (<12msec). The regain of tertiary structure was found to consist of 4 main phases, and a large proportion (80%) of the tertiary structure formed within 2 msec. The regain of riboflavin binding ability was complete at the end of the second phase, a reaction with a half-life of around 30 msec. In the presence and absence of riboflavin, the kinetics for the first 3 stages of tertiary structure changes seemed to be identical. In the presence of riboflavin, however, seemed to impede the completion of the final, very slow stage, with the refolding reaction only going to 95% completion. The dephosphorylation of the protein seemed to have no affect on this process. When the 9 disulphide bonds are reduced however, RfBP is unable to spontaneously reoxidise to a native-like state in the presence of an oxidised/reduced glutathione redox system. However, the addition of protein disulphide isomerase to the system increases significantly the yield of successfully reoxidised RfBP to about 50%. Attempts to prepare deglycosylated RfBP by chemical methods were unsuccessful since the treatment led to fragmentation of the polypeptide chain.

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Libri sul tema "Riboflavin"

Schneeman, Barbara O., Ann L. Yaktine e Alice Vorosmarti, a cura di. Scanning for New Evidence on Riboflavin to Support a Dietary Reference Intake Review. Washington, D.C.: National Academies Press, 2021. http://dx.doi.org/10.17226/26188.

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Madigan, Sharon M. Riboflavin status, intake and inter-relationship with other nutrients in a healthy elderly population in NorthernIreland. [S.l: The Author], 1993.

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1909-, Bourne Geoffrey H., a cura di. Nutritional disorders and requirements. Basel: Karger, 1987.

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Institute of Medicine (U.S.). Standing Committee on the Scientific Evaluation of Dietary Reference Intakes., Institute of Medicine (U.S.). Panel on Folate, Other B Vitamins, and Choline. e Institute of Medicine (U.S.). Subcommittee on Upper Reference Levels of Nutrients., a cura di. DRI, dietary reference intakes for thiamin, riboflavin, niacin, vitamin B₆, folate, vitamin B₁₂, pantothenic acid, biotin, and choline. Washington, D.C: National Academy Press, 1998.

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Hannover, Tierärztliche Hochschule, a cura di. Untersuchungen zu [beta]-Carotin-, Vitamin E-, Thiamin-, Riboflavin- und Vitamin B₆-Gehalten verschiedener fett- beziehungsweise kohlenhydratreicher Einzelfuttermittel für Ziervögel. Hannover: [s.n.], 1999.

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Rivlin, Richard. Riboflavin. Springer, 2011.

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Riboflavin. Springer, 2011.

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Rivlin, Richard. Riboflavin. Springer London, Limited, 2012.

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Gluckman, Sir Peter, Mark Hanson, Chong Yap Seng e Anne Bardsley. Vitamin B2 (riboflavin) in pregnancy and breastfeeding. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780198722700.003.0008.

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Riboflavin is a cofactor for enzymes involved in energy generation, biosynthesis, detoxification, and electron-scavenging pathways, as well as in the metabolism of other B vitamins. Deficiency is rare in developed countries; it is encountered almost invariably in combination with deficit of other B vitamins in areas of poor overall nutrition. Deficiency is endemic in populations whose staple diet consists of rice and wheat, with low or no consumption of meat and dairy products. Infants of riboflavin-deficient mothers tend to be deficient themselves at birth and remain deficient through breastfeeding and weaning. To ensure adequate riboflavin supply in both mother and infant, an increase in dairy products and/or meat consumption, particularly during lactation, may be necessary in some women.

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A. R. (Alexander R. ) Robblee e University of Alberta Dept of Animal. Use of Crystalline Riboflavin in Poultry Rations. Creative Media Partners, LLC, 2021.

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Capitoli di libri sul tema "Riboflavin"

Winter, Jerrold. "Riboflavin". In True Nutrition, True Fitness, 107–14. Totowa, NJ: Humana Press, 1991. http://dx.doi.org/10.1007/978-1-4612-0479-4_8.

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Rivlin, Richard S. "Riboflavin". In Essential Nutrients in Carcinogenesis, 349–55. Boston, MA: Springer US, 1986. http://dx.doi.org/10.1007/978-1-4613-1835-4_26.

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McCormick, Donald B. "Riboflavin". In Present Knowledge in Nutrition, 280–92. Oxford, UK: Wiley-Blackwell, 2012. http://dx.doi.org/10.1002/9781119946045.ch18.

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Bährle-Rapp, Marina. "Riboflavin". In Springer Lexikon Kosmetik und Körperpflege, 476. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_8915.

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Bährle-Rapp, Marina. "Riboflavin Tetraacetate". In Springer Lexikon Kosmetik und Körperpflege, 476. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_8916.

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Schomburg, Dietmar, e Dörte Stephan. "Riboflavin kinase". In Enzyme Handbook 13, 701–6. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-59176-1_137.

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Schomburg, Dietmar, e Dörte Stephan. "Riboflavin phosphotransferase". In Enzyme Handbook 13, 823–25. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-59176-1_153.

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Schomburg, Dietmar, e Dörte Stephan. "Riboflavin synthase". In Enzyme Handbook 13, 39–44. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-59176-1_9.

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Jordan, Douglas B., Karen O. Bacot, Thomas J. Carlson, Michael P. Picollelli, Zdzislaw Wawrzak, Martin Kessel e Paul V. Viitanen. "Riboflavin Biosynthetic Enzymes". In Photosynthesis: Mechanisms and Effects, 3637–40. Dordrecht: Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-3953-3_849.

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Floss, Heinz G., Paul J. Keller, Quang Le Van, Peter Nielsen, Gerhard Neuberger e Adelbert Bacher. "Biosynthesis of Riboflavin". In Natural Product Chemistry, 108–20. Berlin, Heidelberg: Springer Berlin Heidelberg, 1986. http://dx.doi.org/10.1007/978-3-642-71425-2_6.

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Atti di convegni sul tema "Riboflavin"

Li, Fa-Tang, e Rui-Hong Liu. "Photosensitized Oxidative Desulfurization of Thiophene by Riboflavin". In 2009 International Conference on Environmental Science and Information Application Technology, ESIAT. IEEE, 2009. http://dx.doi.org/10.1109/esiat.2009.261.

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Orynbayev, A. T., K. A. Miroshnikov, A. N. Ignatov e F. S. Dzhalilov. "Evaluation of effectiveness of bacteriophage agent for cabbage black rot control". In Растениеводство и луговодство. Тимирязевская сельскохозяйственная академия, 2020. http://dx.doi.org/10.26897/978-5-9675-1762-4-2020-113.

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Twenty-one isolates of bacteriophages specific to eleven target strains of Xanthomonas campestris pv. campestris were isolated from soil samples collected under black rot-infected cabbage plants. After the analysis of phagotyping for seventy-three phytopathogen strains against newly isolated isolates and four collection strains of bacteriophages, it was proposed to construct a phage cocktail including 6 isolates In vitro screening of protective from ultraviolet radiation substances under the UV-B range showed that skim milk (0.75%), Riboflavin (0.5%) and ascorbic acid (0.1%) showed the highest effect for bacteriophages. Under the conditions of a film greenhouse, the best protective effect from solar UV radiation on the 8th day after spraying cabbage was shown by the option with the addition of skimmed milk (0.75%) and Riboflavin (0.5%). According to our data, these substances can provide a long-term photoprotective effect of the bacteriophage preparation..

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Schumacher, S., J. Wernli, S. Scherrer, M. Bueehler, T. Seiler e M. Mrochen. "Optimization model for UV-Riboflavin corneal cross-linking". In SPIE BiOS, a cura di Fabrice Manns, Per G. Söderberg e Arthur Ho. SPIE, 2011. http://dx.doi.org/10.1117/12.876797.

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Yang, Hui, Xue Xiao, Xuesong Zhao, Lan Hu, Junjun Zong e Xiangfeng Xue. "Study on Fluorescence Spectra of Thiamine and Riboflavin". In 3rd International Conference on Material, Mechanical and Manufacturing Engineering (IC3ME 2015). Paris, France: Atlantis Press, 2015. http://dx.doi.org/10.2991/ic3me-15.2015.281.

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Paulson, Bjorn, Inchul Shin, Byungjoo Kong, Gregor Sauer, Sreekantha Reddy Dugasani, Reza Khazaeinezhad, Woohyun Jung, Boram Joo e Kyunghwan Oh. "Preparation and optical characterization of DNA-riboflavin thin films". In SPIE Nanoscience + Engineering, a cura di Norihisa Kobayashi, Fahima Ouchen e Ileana Rau. SPIE, 2016. http://dx.doi.org/10.1117/12.2237516.

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Yang, Hui, Xue Xiao, Xuesong Zhao, Lan Hu, Caofang Lv e Zhangkun Yin. "Study on fluorescence spectra of thiamine, riboflavin and pyridoxine". In Seventh International Symposium on Precision Mechanical Measurements, a cura di Liandong Yu. SPIE, 2016. http://dx.doi.org/10.1117/12.2211248.

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Jasim, Layth S., Aseel M. Aljeboree, Ibrahim J. Sahib, Makarim A. Mahdi, Firas H. Abdulrazzak e Ayad F. Alkaim. "Effective adsorptive removal of riboflavin (RF) over activated carbon". In 3RD INTERNATIONAL SCIENTIFIC CONFERENCE OF ALKAFEEL UNIVERSITY (ISCKU 2021). AIP Publishing, 2022. http://dx.doi.org/10.1063/5.0066996.

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Prince, Payal Kalra e Ekambir Sidhu. "Rectangular TeraHertz microstrip patch antenna design for riboflavin detection applications". In 2017 International Conference on Big Data Analytics and Computational Intelligence (ICBDAC). IEEE, 2017. http://dx.doi.org/10.1109/icbdaci.2017.8070853.

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"Riboflavin Mediated Degradation of Chlorpyrifos by Alcaligens sp. Strain T11". In 5th International Conference on Biological, Chemical and Environmental Sciences. International Institute of Chemical, Biological & Environmental Engineering, 2016. http://dx.doi.org/10.15242/iicbe.c0316014.

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Yang, Hao, Yi Qu, Te Li, Zhibin Zhao, Shuai Yu, Lei Liu, Chaoqun Tian e Donghan Wei. "Si PIN diodes for detecting photoluminescence of NAPDH and riboflavin". In 2012 International Conference on Optoelectronics and Microelectronics (ICOM). IEEE, 2012. http://dx.doi.org/10.1109/icoom.2012.6316337.

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