Bibliografie tematiche / Psuedomonas aeruginosa

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Letteratura scientifica selezionata sul tema "Psuedomonas aeruginosa"

Autore: Grafiati

Pubblicato: 4 giugno 2021

Ultima modifica: 8 febbraio 2022

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Articoli di riviste sul tema "Psuedomonas aeruginosa"

Deng, Yang, Li Li Ji, Lin Li, Bing Li e Jian Yu Su. "Development and Application of a Novel Nucleic Amplification Kit on Detection of Several Pathogens". Applied Mechanics and Materials 618 (agosto 2014): 293–97. http://dx.doi.org/10.4028/www.scientific.net/amm.618.293.

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Escherichia coliO157,Psuedomonas aeruginosa,Salmonella,Vibrio parahaemolyticusandListeriaare important pathogens for human. With increased awareness in public health, development of a rapid, sensitive, cost-effective and easy-operating bacteriological detection is of the utmost importance and urgent necessity. In this study, we developed and applied a simple amplification kit based on loop-mediated isothermal amplification (LAMP) methods for rapid detection of various pathogens includingEscherichia coliO157,Psuedomonas aeruginosa,Salmonella,Vibrio parahaemolyticusandListeria, as well as related virulence. Nine targets, includingrfbE(E. coli-specific),stx1 (coding for Shiga toxin 1),stx2 (coding for Shiga toxin 2),oprI(P. aeruginosa–specific),invA(Salmonella-specific),hlyA(Listeria-specific),tlh(coding for thermolable haemolysin),tdh(coding for thermostable direct haemolysin) andtrh(coding for TDH-related haemolysin), were selected for identification forEscherichia coliO157,Psuedomonas aeruginosa,Salmonella,Vibrio parahaemolyticusandListeria, as well as related virulence.. Six primers, including outer primers, inner primers and loop primers, were specially designed for recognizing eight distinct sequences on the targets. Three solutions labeled A, B and C was included in the kit. The experiment were carried out in a total of 25 μl reaction mixture: solution A containing 1.6 μM (each) of the primers FIP and BIP, 0.2 μM (each) of the primers F3 and B3, 0.8 μM (each) of primers LF and LB; solution B containing 1.6 mM of deoxynucleoside triphosphates, 6 mM MgSO4, 1 M betain (Sigma, St. Louis, MO, USA), 1 X thermopol buffer (New England Biolabs, Ipswich, MA, USA); solution C containing BST polymerase. Twenty-two reference strains, including various species of gram-negative and-positive isolates, were included in this study to evaluate and optimize LAMP assays. Application of the optimized LAMP assays was performed on a total of 200 strains (with 40 strains for each species of the pahtogens). The optimal reaction condition was found to be 65°C for 45 min. Application of the kit assays were performed on various types of pathogens, the sensitivity for the 9 targets was found to be 100%; with a 100% specificity and positive predictive value (PPV) for all the 9 targets targets. In conclusion, the isothermal amplification kits were demonstrated to be useful and powerful tools for rapid differentiation of various pathogens (includingEscherichia coliO157,Psuedomonas aeruginosa,Salmonella,Vibrio parahaemolyticusandListeria), and undoubtedly, the rapidness, easiness and cost-effectiveness of LAMP assay will aid in the broad application of bacteriological detection of common pathogens.

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Omar, Muhammad Nor, Norhayati Shaban, Latifah Bakar e Ahmad Muzammil Zuberdi. "Microbial Trans formation of Clarias gariepinus Oil by Psuedomonas aeruginosa". Oriental Journal of Chemistry 30, n. 3 (26 settembre 2014): 1147–51. http://dx.doi.org/10.13005/ojc/300327.

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Singh, J. K., R. Ranjan e Pranay Pankaj. "Isolation and Screening of Water Microbes for Decolourisation of Textile Dye Waste". Current World Environment 11, n. 1 (25 aprile 2016): 296–300. http://dx.doi.org/10.12944/cwe.11.1.36.

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Azo dyes are widely used in textile industry. Unused dyes, consisting mainly non biodegradable released along with waste water streams without any proper pre-treatment which cause nuisance for environment and accumulate in flora as well as fauna. These also exhibit allergic, carcinogenic and mutagenic properties for human beings. Isolation and screening of azo dye degrading bacteria are economic in biodegradation and detoxification. In the present study, 200 waste water samples were collected from dye-contaminated sites of textile industries and bacterial species such as Bacillus subtilis, Pseudomonas aeruginosa and Psuedomonas putida were isolated and identified. Evaluation of decolorizing properties of these bacteriae were done by UV-Vis spectroscopy (Amax 596 nm) in different concentrations using different carbon sources such as Hans’s medium and GYP medium. Maximum decolourisation of 0.1% azo dyes were recorded to be 89.0%, 91% and 86% in Hans medium containing charcoal source by Bacillus subtilis, Pseudomonas aeruginosa and Psuedomonas putida respectively at 24 hrs. These bacterial isolates may be utilized in large scale for pre-treatment for ecological balance by avoiding water pollution.

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Chih-Jen, Lu, Lee Chi-Mei e Huang Chiou-Zong. "Biodegradation of chlorophenols by immobilized pure-culture microorganisms". Water Science and Technology 34, n. 10 (1 novembre 1996): 67–72. http://dx.doi.org/10.2166/wst.1996.0240.

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The biodegradation of phenol and chlorophenols by immobilized pure-culture cells was conducted by a series of batch reactors. The microorganisms used in this study were Pseudomonas putida, Psuedomonas testosteroni, Pseudomonas aeruginosa, and Agrobacterium radiobacter. All four species showed the ortho-cleavage pathway to metabolize chlorophenols. Among the four species, P. testosteroni, P. putida, and P. aeruginosa could effectively remove phenol at 200 mg/l. P. testosteroni could effectively remove 2-chlorophenol at 10mg/l. However, the other three species, P. putida, P. aeruginosa, and A. radiobacter, could not effectively remove 2-chlorophenol. Although 3-chlorophenol is a recalcitrant compound, P. testosteroni also could rapidly metabolize 3-chlorophenol at 10 mg/l. The removal of 4-chlorophenol at 10 mg/l by P. testosteroni reached 98% within one day. P. aeruginosa and A. radiobacter also could metabolize 4-chlorophenol after 2 and 7 days of lag period, respectively.

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Rahman Rasheed Al-Taai, Hadi, Zainab Mohammed Hameed e Izdehar Mohammed Jasim. "Investigation on bla Ctx-M-1 , Virulence Factors and Multidruge Resistance in Pseudomonas Aeruginosa Bacteria". Diyala Journal For Pure Science 13, n. 3 (1 luglio 2017): 206–21. http://dx.doi.org/10.24237/djps.1303.283c.

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V. Eugin Amala, V. Eugin Amala, e Dr M. Jeyaraj Dr. M. Jeyaraj. "Phytochemical, Antibacterial and Functional Group Identification of Medicinally Useful Plant Terminalia chebula Retz., against Staphylococcus aureus, Psuedomonas aeruginosa and Klebsiella pneumoniae". Indian Journal of Applied Research 4, n. 1 (1 ottobre 2011): 23–25. http://dx.doi.org/10.15373/2249555x/jan2014/8.

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Yung, H., e J. Parmar. "S137 De novo psuedomonas aeruginosa colonisation is associated with increased acute rejection and bronchiolitis obliterans syndrome following lung transplantation". Thorax 71, Suppl 3 (15 novembre 2016): A81—A83. http://dx.doi.org/10.1136/thoraxjnl-2016-209333.143.

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Thamlikitkul, Visanu, Surapee Tiengrim, Narisara Thamthaweechok, Preeyanuch Buranapakdee e Wilai Chiemchaisri. "Contamination by Antibiotic-Resistant Bacteria in Selected Environments in Thailand". International Journal of Environmental Research and Public Health 16, n. 19 (5 ottobre 2019): 3753. http://dx.doi.org/10.3390/ijerph16193753.

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This study determined the presence of important antibiotic-resistant bacteria in selected environments in Thailand, including wastewater samples from 60 hospitals; washed fluid, leachate, flies, cockroaches, and rats collected from five open markets; washed fluid from garbage trucks; and stabilized leachate from a landfill facility. At least one type of antibiotic-resistant bacteria was isolated from all samples of influent fluid before treatment in hospitals, from wastewater treatment tank content in hospitals, and from 15% of effluent fluid samples after treatment with chlorine prior to draining it into a public water source. Antibiotic-resistant bacteria were recovered from 80% of washed market fluid samples, 60% of market leachate samples, all fly samples, 80% of cockroach samples, and all samples of intestinal content of rats collected from the open markets. Antibiotic-resistant bacteria were recovered from all samples from the landfill. Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and/or Klebsiella pneumoniae were the most common antibiotic-resistant bacteria recovered from all types of samples, followed by carbapenem-resistant E. coli and/or K. pneumoniae. Colistin-resistant Enterobacteriaceae, carbapenem-resistant Psuedomonas aeruginosa, carbapenem-resistant Acinetobacter baumannii, colistin-resistant Enterobacteriaceae, vancomycin-resistant Enterococci, and methicillin-resistant S. aureus were less common. These findings suggest extensive contamination by antibiotic-resistant bacteria in hospital and community environment in Thailand.

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Sherwani1, Neelam, Raeid M. M. Abed, Sergey Dobretsov e Sheji Mary. "Antibacterial and Antifungal Activities of Cyanobacterial Strains Isolated from Hot Springs in Oman". Sultan Qaboos University Journal for Science [SQUJS] 20, n. 1 (1 giugno 2015): 11. http://dx.doi.org/10.24200/squjs.vol20iss1pp11-19.

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In this study, cyanobacterial microbial mats from five hot springs in Oman, namely Al Kasfah Rustaq, Al Thwara Nakhl, Al–Ali Hammam, Gala and Bowsher, were characterized using direct microscopy. Nine monoclonal cyanobacterial cultures were obtained and their extracts in butanol, dichloromethane (DCM) and hexane were screened for antibacterial and antifungal activities. Direct microscopy revealed the presence of 12 different unicellular and filamentous morphotypes, with different distribution in the various mats. Temperature seems to be one of the most important parameters that accounts for the differences in cyanobacterial composition of the mats. Cells of the nine isolates and their aqueous supernatants were subsequently extracted with butanol, DCM and hexane. Dried extracts were tested against nine bacterial (i.e. gram +ve Staphylococcus aureus, Bacillus subtilis and gram –ve, Escherichia coli, Klebsiella pneumoniae, Salmonella choleraesuis, S. enterica, Psuedomonas aeruginosa, Providencia stuartii, and Acinetobacter calcoaceticus) and two fungal pathogens (Rhizoctonia solani and Pythium sp.). All isolates exhibited antibacterial and antifungal activities, which depended mainly on the type of cyanobacterial culture, type of solvent used and the pathogen tested. The highest antibacterial activity was observed in Phormidium species, and butanol was found to be the most appropriate solvent to extract bioactivity from these cyanobacterial species. The results of this study suggest that thermal springs in Oman harbor diverse types of cyanobacteria, which may constitute an important source of antibacterial and antifungal compounds. Further investigation is needed to purify these compounds and find their chemical compositions and modes of action.

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Yu, Guangchao, Wangrong Wen, Brian M. Peters, Junyan Liu, Congxiu Ye, Yuchuan Che, Juzhen Liu, Kaiyuan Cao, Zhenbo Xu e Mark E. Shirtliff. "First report of novel genetic array aacA4 - bla IMP-25 - oxa30 - catB3 and identification of novel metallo-β-lactamase gene bla IMP25 : A Retrospective Study of antibiotic resistance surveillance on Psuedomonas aeruginosa in Guangzhou of South China, 2003–2007". Microbial Pathogenesis 95 (giugno 2016): 62–67. http://dx.doi.org/10.1016/j.micpath.2016.02.021.

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Tesi sul tema "Psuedomonas aeruginosa"

Mugnier, Pierre. "Beta-lactamases a spectre etendu chez psuedomonas aeruginosa". Paris 11, 1996. http://www.theses.fr/1996PA114848.

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Ritchie, Adam John Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "Modulation of T cell responses by N-(3-oxododecanoyl)-L-homoserine lactone". Awarded by:University of New South Wales. Biotechnology and Biomolecular Sciences, 2005. http://handle.unsw.edu.au/1959.4/22005.

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Abstract (sommario):

In Pseudomonas aeruginosa, which causes severe secondary infections in immunocompromised patients, virulence factor expression is regulated by quorum sensing signal molecules known as acyl homoserine lactones (AHLs). One of the major AHLs produced by P. aeruginosa, N-(3-oxododecanoyl)-L-homoserine lactone (OdDHL), has also been shown to alter the function of a range of mammalian cells. The goals of experiments reported in this thesis were to use murine models to investigate the effects of in vivo exposure to OdDHL on TH responses, define the direct effects of OdDHL on TH cells and to explore the mechanism by which OdDHL alters the function of TH cells. It was found that in vivo exposure to OdDHL led to changes in cytokine and antibody subclass production indicative of a shift towards the underlying TH bias of the mouse strain studied. Such shifts may play a role in infections with P. aeruginosa, as strong TH1 or TH2 responses have been associated with worsening prognosis for the host, while more balanced responses have been associated with decreases in both infection and pathology. These results suggest that treatments targeting the immunomodulatory activities of OdDHL may be of benefit in the clinical setting in the future. Direct analysis of TH cells in defined in vitro systems revealed that exposure to OdDHL led to uniform decreases in cytokine production and proliferation. These decreases in cytokine production were found to be the result of OdDHL acting on both TH cells and the antigen presenting cells (APCs) that activate them, and only occurred when cells were exposed to OdDHL within 4 hours of stimulation. These findings suggest that OdDHL is acting on a molecular target common to several cells types, and that in TH cells and APCs, this target is involved in the early stages of TH cell activation. Experiments in which T cells were activated with mitogens that bypass the cell membrane revealed that OdDHL is not acting on the cell membrane or membrane-associated activation factors, suggesting that OdDHL is instead inhibiting TH cell function through interactions with one or more intracellular signalling molecules.

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Wong, Kendy Kit-Ying. "Molecular studies of the structure-function relationships of Psuedomonas aeruginosa OprM : an outer membrane protein associated with efflux". Thesis, 2001. http://hdl.handle.net/2429/13513.

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Abstract (sommario):

Pseudomonas aeruginosa demonstrates high intrinsic resistance to multiple classes of structurally unrelated antimicrobial agents. This broad-spectrum resistance is primarily due to a combination of low outer membrane permeability coupled to secondary resistance mechanisms such as the resistance-nodulation-division (RND) efflux systems. The outer membrane protein OprM is involved in intrinsic and mutational multiple-antibiotic resistance as part of two RND efflux systems in P. aeruginosa. To study structure-function relationships for OprM, optimal conditions for oprM overexpression were determined; it appeared that excessive production of the protein was lethal to cells. In addition, overexpression of oprM alone in wild-type P. aeruginosa did not increase the resistance of the cells, suggesting OprM could not function independent of the pump and linker proteins of the efflux systems. OprM was demonstrated for the first time to have channel-forming activities like porins, and was shown to be cation selective like the related TolC protein of Escherichia coli. Based on the functional and sequence similarity between OprM and TolC, and their similar content of a-helical and P-sheet structures determined by circular dichroism spectroscopy, a three-dimensional model of OprM was constructed by threading its sequence to the TolC crystal structure. This suggested that, like TolC, OprM has a distinctive architecture comprising outer membrane p-barrel and periplasmic helical-barrel structures. Analyses of OprM insertion and deletion mutants in the context of this model indicated that the helical barrel is critical for both function and integrity of OprM, while a C-terminal domain localized around the equatorial plane of this helical barrel is dispensable. Unlike the classic porins, extracellular loops appear to play a minimal role in substrate specificity. There appears to be a correlation between the change in antimicrobial activity for certain OprM mutants and the channel size determined by planar bilayer analysis, supporting the "iris" mechanism of action first suggested for TolC. This study provids information on the structure-function relationships of OprM in a three-dimensional context, and will allow more focused hypotheses and studies about the functional domains of OprM and its related family of efflux proteins.

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Capitoli di libri sul tema "Psuedomonas aeruginosa"

Mazhar Ali, Nazish, Safia Rehman, Syed Abdullah Mazhar, Iram Liaqat e Bushra Mazhar. "Psuedomonas aeruginosa-Associated Acute and Chronic Pulmonary Infections". In Pathogenic Bacteria. IntechOpen, 2020. http://dx.doi.org/10.5772/intechopen.93504.

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Pseudomonas aeruginosa is highly successful in colonizing in all types of environments. P. aeruginosa colonizing in adverse environment due to the presence of its virulence factors include production of toxins, proteases hemolysins, and formation of biofilms. In man, the most common opportunist pathogen is P. aeruginosa. Metabolically P. aeruginosa is versatile. Most of the antibiotics targeted metabolically active cells and bacteria could contribute to decrease in biofilm susceptibility to the antimicrobial agents. Scientists suggested about Pseudomonas that it can be catabolized any hydrocarbon in specific time along with availability of oxygen and nitrite. If bacteria are not susceptible to one agent in three or more, it is called as multidrug-resistance strains. The antimicrobial treatments were not suitable when microorganism presented in vitro microorganism resistance to antimicrobials used for treatment of the patient which lack of treatment for 24 h after diagnosis of microbial infections. Bacteria have developed resistance against commonly used antibiotics. Treatment of Pseudomonas infections is coming harder day by day as its resistance against most of the antibiotics. Because of resistance of bacteria antibiotics, alternative methods are in consideration. These methods include use of lactic acid bacteria (LAB) and most recently nano-particles. That is why they are used as antibacterial agents.

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Atti di convegni sul tema "Psuedomonas aeruginosa"

Hirao, Akihiro, Shunichi Sato, Mitsuhiro Terakawa, Daizoh Saitoh, Nariyoshi Shinomiya, Hiroshi Ashida e Minoru Obara. "In vivo photodynamic inactivation of Psuedomonas aeruginosa in burned skin in rats". In BiOS, a cura di David H. Kessel. SPIE, 2010. http://dx.doi.org/10.1117/12.841495.

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