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1

Orive, Emma, Lara Pérez-Aicua, Helena David, Koldo García-Etxebarria, Aitor Laza-Martínez, Sergio Seoane e Irati Miguel. "The genus Pseudo-nitzschia (Bacillariophyceae) in a temperate estuary with description of two new species: Pseudo-nitzschia plurisecta sp. nov. and Pseudo-nitzschia abrensis sp. nov." Journal of Phycology 49, n. 6 (11 novembre 2013): 1192–206. http://dx.doi.org/10.1111/jpy.12130.

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Gai, Frederik Frøsig, Cecilie Kirketerp Hedemand, Deon C. Louw, Kolette Grobler, Bernd Krock, Øjvind Moestrup e Nina Lundholm. "Morphological, molecular and toxigenic characteristics of Namibian Pseudo-nitzschia species – including Pseudo-nitzschia bucculenta sp. nov." Harmful Algae 76 (giugno 2018): 80–95. http://dx.doi.org/10.1016/j.hal.2018.05.003.

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Riouchi, Ouassila, Faid El Madani, Eric Abadie, Ali Skalli, Mustapha Akodad, Abdelmajid Moumen, Hanane Ait Hmeid, Ghizlane Azizi e Mourad Baghour. "Pseudo-Nitzschia sp (Bacillariophyceae), seasonal distribution in the lagoon of Nador (Morroco)". E3S Web of Conferences 314 (2021): 01003. http://dx.doi.org/10.1051/e3sconf/202131401003.

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The goal of this study is to look into the dominance of diatoms, specifically the genus “Pseudo-Nitzschia Sp” at the Nador lagoon level and how it relates to the physicochemical parameters of the environment. From the four sampling stations and for two seasons (spring and summer 2018). This diatom of the genus “Pseudo-Nitzschia Sp” includes toxic species capable of producing domoic acid (DA), a neurotoxin responsible for amnesic intoxication syndrome in humans. During sampling, the species “PseudoNitzschia Sp” showed variable cell densities between stations and seasons. The dominate microalgae were observed during the spring period with a maximum concentration of (4000 Cells / l). And a low viscosity during the summer seasons (140 Cells / l).
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Ramili, Yunita, Halikuddin Umasangaji e Andreyan Drakel. "Composition and Abundance of Phytoplankton with Potential Harmful Algal Blooms (HABs) on Ternate Island Coastal Waters, North Maluku". Agrikan Jurnal Agribisnis Perikanan 16, n. 1 (13 maggio 2023): 83–93. http://dx.doi.org/10.52046/agrikan.v16i1.1519.

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The existence of several species of phytoplankton with potential HABs (Harmful Algal Blooms) was investigated on the coastal waters of Ternate Island. This work aims at describing the distribution, species composition, and the abundance of phytoplankton with potential HABs and their ecological index. Data collection was carried out in August 2022 in the coastal waters of Ternate Island at three research locations, namely in Dorpedu (Station 1), Falajawa, Muhajirin Village (Station 2) and Kulaba (Station 3), respectively. Sampling of phytoplankton uses a filtering method with a plankton net. The results of this study quantified 24 genera of phytoplankton where 11 generas were included the group of potential HABs, namely Leptocylindrus, Coscinodiscus, Pseudo Nitzschia. Rhizosolenia, Chaetoceros, and Nitzschia (Class Bacillariophyceae), Gymnodinium, Protoperidinium, Alexandrium, and Ceratium (Class Dinophyceae), and Phaeocystis (Class Prymnesiophyte). The highest abundance of potential HABs varied between research stations, Rhizosolenia sp. of 7.6388x104 cells/l at station 1, Leptocylindrus sp. of 5.4504x104 cells/l, Phaeocystis sp. of 4.0x104 cells/l at Station 2. On the other hand, the lowest abundance was Nitzschia sp. of 4.1291x102 cells/l at Station 1, Gymnodinium sp. of 4.1291x102 cells/l at Station 2, and Pseudo Nitzschia sp. of 1.2387x103 cells/l at Station 3. Generally, the ecological condition of the phytoplankton community in Ternate Island Waters has a moderate level of species diversity with fairly even distribution of species and no dominance of certain species.
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Percopo, Isabella, Maria Valeria Ruggiero, Sergio Balzano, Priscillia Gourvil, Nina Lundholm, Raffaele Siano, Anna Tammilehto, Daniel Vaulot e Diana Sarno. "Pseudo-nitzschia arctica sp. nov., a new cold-water cryptic Pseudo-nitzschia species within the P. pseudodelicatissima complex". Journal of Phycology 52, n. 2 (8 marzo 2016): 184–99. http://dx.doi.org/10.1111/jpy.12395.

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Polyakova, S. L., O. I. Davidovich, Yu A. Podunay e N. A. Davidovich. "Modification of the ESAW culture medium used for cultivation of marine diatoms". Marine Biological Journal 3, n. 2 (29 giugno 2018): 73–80. http://dx.doi.org/10.21072/mbj.2018.03.2.06.

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Experiments with clonal cultures of four pennate diatoms Haslea karadagensis, H. ostrearia, Pleurosigma sp. and Pseudo-nitzschia cf. seriata revealed that adding of sodium thiosulfate to the culture medium resulted in increase of algae growth rate. The highest, approximately two-fold, increase of the growth rate was observed in Pseudo-nitzschia cf. seriata, a member of the genus including toxicogenous species. Dependence of the algae growth rate on concentration of sodium thiosulfate was studied; optimal concentrations providing the highest growth rate were determined. Addition of ferrous-ammonium citrate or replacement of ferric chloride with ferrous-ammonium citrate did not affect the growth rate of the diatoms studied. For practical reasons, it is useful to prepare culture medium with the salinity of 36 ‰. Modified recipe of the ESAW medium is given.
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Lundholm, Nina, Stephen S. Bates, Keri A. Baugh, Brian D. Bill, Laurie B. Connell, Claude Léger e Vera L. Trainer. "CRYPTIC AND PSEUDO-CRYPTIC DIVERSITY IN DIATOMS-WITH DESCRIPTIONS OF PSEUDO-NITZSCHIA HASLEANA SP. NOV. AND P. FRYXELLIANA SP. NOV.1". Journal of Phycology 48, n. 2 (19 marzo 2012): 436–54. http://dx.doi.org/10.1111/j.1529-8817.2012.01132.x.

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8

Hong, Zhuan, Qiliang Lai, Qiaoqi Luo, Simeng Jiang, Ruilin Zhu, Junrong Liang e Yahui Gao. "Sulfitobacter pseudonitzschiae sp. nov., isolated from the toxic marine diatom Pseudo-nitzschia multiseries". International Journal of Systematic and Evolutionary Microbiology 65, Pt_1 (1 gennaio 2015): 95–100. http://dx.doi.org/10.1099/ijs.0.064972-0.

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A taxonomic study was carried out on bacterial strain H3T, which was isolated from the toxic marine diatom Pseudo-nitzschia multiseries. Cells of strain H3T were Gram-stain-negative, rod-shaped, non-motile and capable of reducing nitrate to nitrite, but not denitrification. Growth was observed at NaCl concentrations of 1–9 %, pH 6–12 and 10–37 °C. It was unable to degrade aesculin or gelatin. The dominant fatty acids (>10 %) were C18 : 1ω7c/ω6c (summed feature 8) and C16 : 0. The respiratory ubiquinone was Q10. The major lipids were phosphatidylethanolamine, phosphatidylglycerol, an aminolipid and one unknown lipid, and the minor lipids were two phospholipids and three unknown lipids. The G+C content of the chromosomal DNA was 61.7 mol%. 16S rRNA gene sequence comparison showed that strain H3T was related most closely to Sulfitobacter donghicola DSW-25T (97.3 % similarity) and levels of similarity with other species of the genus Sulfitobacter were 95.1–96.9 %. The mean (±sd) DNA–DNA hybridization value between strain H3T and Sulfitobacter donghicola DSW-25T was 18.0±2.25 %. The average nucleotide identity between strain H3T and Sulfitobacter donghicola DSW-25T was 70.45 %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain H3T formed a separate clade close to the genus Sulfitobacter and was distinguishable from phylogenetically related species by differences in several phenotypic properties. On the basis of the phenotypic and phylogenetic data, strain H3T represents a novel species of the genus Sulfitobacter , for which the name Sulfitobacter pseudonitzschiae is proposed (type strain H3T = DSM 26824T = MCCC 1A00686T).
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9

Lundholm, Nina, e Øjvind Moestrup. "The marine diatom Pseudo-nitzschia galaxiae sp. nov. (Bacillariophyceae): morphology and phylogenetic relationships". Phycologia 41, n. 6 (novembre 2002): 594–605. http://dx.doi.org/10.2216/i0031-8884-41-6-594.1.

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10

von Dassow, Peter, Marta Mikhno, Isabella Percopo, Valentina Rubio Orellana, Víctor Aguilera, Gonzalo Álvarez, Michael Araya et al. "Diversity and toxicity of the planktonic diatom genus Pseudo-nitzschia from coastal and offshore waters of the Southeast Pacific, including Pseudo-nitzschia dampieri sp. nov." Harmful Algae 130 (dicembre 2023): 102520. http://dx.doi.org/10.1016/j.hal.2023.102520.

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11

Polykova, Svetlana L., Nickolai A. Davidovich, Olga I. Davidovich e Yuliia A. Podunai. "The effect of sodium thiosulfate in a culture medium on the sexual reproduction of three species of Bacillariophyta". Issues of modern algology (Вопросы современной альгологии), n. 1(25) (2021): 10–14. http://dx.doi.org/10.33624/2311-0147-2021-1(25)-10-14.

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It was studied the effect of sulfur in the form of sodium thiosulfate pentahydrate on the capability of clonal cultures of Pleurosigma sp., Toxarium undulatum, Pseudo-nitzschia calliantha to interbreed successfully and produce fertile offspring if they were cultivated in artificially prepared marine water (ESAW medium). Differences in the time of the beginning of the sexual process and the intensity of its passage for clones contained on ESAW with the addition of sodium thiasulfate and in the absence of it are shown.
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12

Li, Yang, Chun Xiu Huang, Guo Shuang Xu, Nina Lundholm, Sing Tung Teng, Haiyan Wu e Zhijun Tan. "Pseudo-nitzschia simulans sp. nov. (Bacillariophyceae), the first domoic acid producer from Chinese waters". Harmful Algae 67 (luglio 2017): 119–30. http://dx.doi.org/10.1016/j.hal.2017.06.008.

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13

Wolf, Christian, Stephan Frickenhaus, Estelle S. Kilias, Ilka Peeken e Katja Metfies. "Regional variability in eukaryotic protist communities in the Amundsen Sea". Antarctic Science 25, n. 6 (16 aprile 2013): 741–51. http://dx.doi.org/10.1017/s0954102013000229.

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AbstractWe determined the composition and structure of late summer eukaryotic protist assemblages along a west–east transect in the Amundsen Sea. We used state-of-the-art molecular approaches, such as automated ribosomal intergenic spacer analysis (ARISA) and 454-pyrosequencing, combined with pigment measurements via high performance liquid chromatography (HPLC) to study the protist assemblage. We found characteristic offshore and inshore communities. In general, total chlorophyll a and microeukaryotic contribution were higher in inshore samples. Diatoms were the dominant group across the entire area, of which Eucampia sp. and Pseudo-nitzschia sp. were dominant inshore and Chaetoceros sp. was dominant offshore. At the most eastern station, the assemblage was dominated by Phaeocystis sp. Under the ice, ciliates showed their highest and haptophytes their lowest abundance. This study delivers a taxon detailed overview of the eukaryotic protist composition in the Amundsen Sea during the summer 2010.
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14

Li, Yang, Huan Chang Dong, Sing Tung Teng, Stephen S. Bates e Po Teen Lim. "Pseudo-nitzschia nanaoensis sp. nov. (Bacillariophyceae) from the Chinese coast of the South China Sea". Journal of Phycology 54, n. 6 (22 ottobre 2018): 918–22. http://dx.doi.org/10.1111/jpy.12791.

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15

Teng, Sing Tung, Hong Chang Lim, Po Teen Lim, Viet Ha Dao, Stephen S. Bates e Chui Pin Leaw. "Pseudo-nitzschia kodamae sp. nov. (Bacillariophyceae), a toxigenic species from the Strait of Malacca, Malaysia". Harmful Algae 34 (aprile 2014): 17–28. http://dx.doi.org/10.1016/j.hal.2014.02.005.

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Amato, Alberto, e Marina Montresor. "Morphology, phylogeny, and sexual cycle of Pseudo-nitzschia mannii sp. nov. (Bacillariophyceae): a pseudo-cryptic species within the P. pseudodelicatissima complex". Phycologia 47, n. 5 (10 settembre 2008): 487–97. http://dx.doi.org/10.2216/07-92.1.

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Pan, Y., ML Parsons, M. Busman, PDR Moeller, Q. Dortch, CL Powell e GJ Doucette. "Pseudo-nitzschia sp. cf. pseudodelicatissima - a confirmed producer of domoic acid from the northern Gulf of Mexico". Marine Ecology Progress Series 220 (2001): 83–92. http://dx.doi.org/10.3354/meps220083.

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18

Bretherton, Laura, Jessica Hillhouse, Hernando Bacosa, Samantha Setta, Jennifer Genzer, Manoj Kamalanathan, Zoe V. Finkel e Antonietta Quigg. "Growth dynamics and domoic acid production of Pseudo-nitzschia sp. in response to oil and dispersant exposure". Harmful Algae 86 (giugno 2019): 55–63. http://dx.doi.org/10.1016/j.hal.2019.05.008.

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Sitel, Ferdaous, Belahcen Rijalebled e Hassan Er-Raioui. "Harmful micro-algal blooms and local environmental conditions of the coastal fringe: Moroccan Mediterranean coasts as a case study". E3S Web of Conferences 234 (2021): 00043. http://dx.doi.org/10.1051/e3sconf/202123400043.

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The dynamic study of harmful phytoplankton, conducted from June 2013 to May 2014, has tracked the evolution of the microalgae community at four sites located along the Moroccan western Mediterranean coast. 91 species and genera of phytoplankton were identified, 8 of which are recognized as potentially toxic. The genus Pseudo-nitzschia, a diatom known to produce domoic acid, is quite prevalent in all four sites, particularly in spring. The toxic dinoflagellate species detected in our study are Gymnodinium catenatum, Alexandrium sp PSP producers, Prorocentruml Lima, Dinophysis acuminata, D. caudata, D. fortii DSP producer, and Ostreopsis sp known palytoxin producer. The species Gymnodinium catenatum proliferates intensively from S1 to S3 in winter and S4 in summer, while Alexandrium sp proliferates mainly during spring and late summer with an exceedance of the threshold. Significant blooms of Dinophysis sp were recorded during the summer at all sites. On the other hand, the abundance of Ostreopsis sp was noted during the spring summer period with low concentrations. Discriminant analysis (DFA) of nutrients, toxic species identifies perfect seasonal discrimination. Winter and fall are characterized by high nutrient inputs, but algal biomass is low. On the contrary, spring and summer are characterized by a depletion of nutrients in the environment following the assimilation of these elements by phytoplankton.
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Huang, Chun Xiu, Huan Chang Dong, Nina Lundholm, Sing Tung Teng, Guan Chao Zheng, Zhi Jun Tan, Po Teen Lim e Yang Li. "Species composition and toxicity of the genus Pseudo-nitzschia in Taiwan Strait, including P. chiniana sp. nov. and P. qiana sp. nov." Harmful Algae 84 (aprile 2019): 195–209. http://dx.doi.org/10.1016/j.hal.2019.04.003.

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Teng, Sing Tung, Po Teen Lim, Hong Chang Lim, María Rivera-Vilarelle, Sonia Quijano-Scheggia, Yoshinobu Takata, Michael A. Quilliam, Matthias Wolf, Stephen S. Bates e Chui Pin Leaw. "A non-toxigenic but morphologically and phylogenetically distinct new species of Pseudo-nitzschia , P. sabit sp. nov. (Bacillariophyceae)". Journal of Phycology 51, n. 4 (14 luglio 2015): 706–25. http://dx.doi.org/10.1111/jpy.12313.

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Delegrange, A., A. Lefebvre, F. Gohin, L. Courcot e D. Vincent. "Pseudo-nitzschia sp. diversity and seasonality in the southern North Sea, domoic acid levels and associated phytoplankton communities". Estuarine, Coastal and Shelf Science 214 (dicembre 2018): 194–206. http://dx.doi.org/10.1016/j.ecss.2018.09.030.

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Klisarova, D., e D. Gerdzhikov. "Phytoplankton blooms in front of cape Galata (Black Sea) during the period 2008-2017". Agricultural Science and Technology 12, n. 1 (marzo 2020): 55–60. http://dx.doi.org/10.15547/ast.2020.01.010.

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Abstract. The aim of the study was to identify the peculiarities of the development of phytoplankton blooms (annual and seasonal dynamics) in front of cape Galata, Bulgarian Black Sea coast in the period 2008-2017. In that area up to 30 nautical miles (nm), from 6 stations (G-1, G-3, G-5, G-10, G-20 and G-30, respectively at 1, 3, 5, 10, 20 and 30 nm from the coast), 187 phytoplankton samples were collected. A total of six phytoplankton species were recorded, developing with the following blooming concentrations: Emiliania huxleyi, Oscillatoria sp., Merismopedia sp., small Flagellates (Cryptophyceae), Prorocentrum cordatum (minimum) and Pseudo-nitzschia delicatissima. It was found that the reported in previous periods trend of increasing phytoplankton biomass in front of cape Galata had already changed. Compared to the observed quantities in the period 1993-2007, the current phytoplankton values in the surface layer (up to 30 nm in front of cape Galata) were 1.63 times lower in abundance and 5.60 times lower in biomass.
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Lim, Hong Chang, Sing Tung Teng, Chui Pin Leaw e Po Teen Lim. "Three novel species in the Pseudo-nitzschia pseudodelicatissima complex: P . batesiana sp. nov., P. lundholmiae sp. nov., and P. fukuyoi sp. nov. (Bacillariophyceae) from the Strait of Malacca, Malaysia". Journal of Phycology 49, n. 5 (27 agosto 2013): 902–16. http://dx.doi.org/10.1111/jpy.12101.

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Esenkulova, Svetlana, Ben J. G. Sutherland, Amy Tabata, Nicola Haigh, Christopher M. Pearce e Kristina M. Miller. "Comparing metabarcoding and morphological approaches to identify phytoplankton taxa associated with harmful algal blooms". FACETS 5, n. 1 (1 gennaio 2020): 784–811. http://dx.doi.org/10.1139/facets-2020-0025.

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Molecular techniques are expected to be highly useful in detecting taxa causing harmful algal blooms (HABs). This is the first report in Canada evaluating HABs-related species identification using a combination of morphological and molecular approaches. Microscopy, quantitative polymerase chain reaction (qPCR), and metabarcoding with multiple markers (i.e., 16S, 18S-dinoflagellate and 18S-diatom, large subunit (28S) rDNA) were applied on samples ( n = 54) containing suspected harmful algae (e.g., Alexandrium spp., Chattonella sp., Chrysochromulina spp., Dictyocha spp., Heterosigma akashiwo, Protoceratium reticulatum, Pseudochattonella verruculosa, Pseudo-nitzschia spp., Pseudopedinella sp.). Owing to methodology limitations, qPCR result interpretation was limited, although good detectability occurred using previously published assays for Alexandrium tamarense, H. akashiwo, and P. verruculosa. Overall, the multiple-marker metabarcoding results were superior to the morphology-based methods, with the exception of taxa from the silicoflagellate group. The combined results using both 18S markers and the 28S marker together closely corresponded with morphological identification of targeted species, providing the best overall taxonomic coverage and resolution. The most numerous unique taxa were identified using the 18S-dinoflagellate amplicon, and the best resolution to the species level occurred using the 28S amplicon. Molecular techniques are therefore promising for HABs taxa detection but currently depend on deploying multiple markers for metabarcoding.
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Ajani, Penelope A., Arjun Verma, Malwenn Lassudrie, Martina A. Doblin e Shauna A. Murray. "A new diatom species P. hallegraeffii sp. nov. belonging to the toxic genus Pseudo-nitzschia (Bacillariophyceae) from the East Australian Current". PLOS ONE 13, n. 4 (12 aprile 2018): e0195622. http://dx.doi.org/10.1371/journal.pone.0195622.

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Diniarti, Nanda, Muhammad Junaidi e Baiq Hilda Astriana. "POTENSI BUDIDAYA IKAN BANDENG SEBAGAI PEMANFAATAN PLANKTON DI PERAIRAN BATU NAMPAR". Indonesian Journal Of Aquaculture Medium 1, n. 1 (23 giugno 2021): 41–48. http://dx.doi.org/10.29303/mediaakuakultur.v1i1.133.

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Perairan Teluk Ekas telah memiliki konsentrasi nutrien yang di atas ambang batas. Nutrien berasal dari pakan ikan rucah yang diberikann oleh pembudidaya ikan sistem Karamba jaring Apung. Nutrien yang berlebihan di perairan akan memicu pertumbuhan besar-besaran plankton atau yang dikenal dengan blooming. Blooming plankton akan berdampak buruk pada biota yang dibudidayakan serta untuk semua organisme yang mendiami perairan tersebut. Plankton yang tumbuh dapat dikendalikan dengan memberikan organisme yang dapat memanfaatkan keberadaannya sebagai pakan. Bandeng banyak digunakan sebagai pemanen /pemanfaatan plankton baik di perairan tawar maupun laut. Bandeng merupakan ikan euryhalin atau memiliki rentang salinitas yang luas. Selain itu ikan bandeng merupakan ikan ekonomis penting. Namun sebelum mengintroduksi ikan bandeng di KJA Batu Nampar perlu dilakukan analisa kesesuaian beberapa parameter lingkungan dan yang tidak kalah pentingnya adalah jenis plankton yang bisa dimanfaatkan oleh bandeng. Oleh karena itu tujuan penelitian ini adalah mengiventaris/mendata jenis plankton di perairan Batu Nampar serta melihat kesesuaian jenis plankton yang ada dengan yang menjadi pakan ikan alami Bandeng. Metode yang digunakan dalam penelitian ini adalah metode survey. Pengamatan lebih dititikberatkan pada pendataan jenis plankton. Pengambilan contoh air dan pengamatan terhadap parameter-parameter kualitas air lainnya dilakukan pada tiap bulan selama 3 kali. Jenis plankton yang teriventarisir pada saat penelitian adalah: Synedra ulna, Fragilaria, Hemiaulus sinensis, Skeletonema costatum, Triceratium taves, Coscinodiscus granii, Pseudo nitzschia, Dytilum sol, Cerataulina smithii, Clamydocapsa sp, Navicula elegans, Aulacodiscus gracilis, Cydotella sp., Globorotolia pumilio, Ceratium sp.,larva crustacean, Pontellina plumata. Plankton yang terdapat lebih banyak dari Bacillariophyceae yang merupakan jenis pakan dari ikan bandeng sehingga perairan KJA Batu Nampar berpotensi digunakan untuk budidaya Bandeng
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Cintra, A. K. A., M. R. Firdaus, N. Fitriya e A. Rachman. "Epibiont of venus clam (Gafrarium tumidum) shells: a study on Pari Island, Seribu Islands". IOP Conference Series: Earth and Environmental Science 1271, n. 1 (1 dicembre 2023): 012019. http://dx.doi.org/10.1088/1755-1315/1271/1/012019.

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Abstract Molluscs play a crucial role as ecosystem engineer by providing habitat for other organisms. However, little is known about the shells’ ability to host other species, especially in tropical ecosystems like Pari Island. In sandy locations, hard substrates are scarce, but clam shells introduce substrate heterogeneity. Our study focused on the Venus Clam (Gafrarium tumidum), prevalent in the seagrass bed ecosystem on Pari Island, to explore the epibiont species residing on its shells. Epibionts were examined through visual observation and microscopy analysis in a laboratory. We found the attached epibionts were categorized into three main groups. The first group was macroalgae, specifically Padina sp., which attached to the posterior part of the clam shell, relying on stable substrates to withstand waves. The second group encompassed microalgae, including Bacillaria, Nitzchia, Prorocentrum, Pseudo-nitzschia, Surirella, and Trichodesmium. These microalgae have diverse ecological roles, including causing harmful algal blooms and nitrogen fixation. The third group consisted of possibly cyanobacteria forming blue-green biofilms on clam shells, predominantly on recently deceased or living clams, mainly in exposed, sunlit areas. This research emphasizes the significant role of clam shells in supporting epibiont species richness in the ecosystem.
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Quijano-Scheggia, Sonia Isabel, Esther Garcés, Nina Lundholm, Øjvind Moestrup, Karl Andree e Jordi Camp. "Morphology, physiology, molecular phylogeny and sexual compatibility of the cryptic Pseudo-nitzschia delicatissima complex (Bacillariophyta), including the description of P. arenysensis sp. nov." Phycologia 48, n. 6 (novembre 2009): 492–509. http://dx.doi.org/10.2216/08-21.1.

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Vanmaldergem, Jean, José Luis García-Corona, Margot Deléglise, Caroline Fabioux e Hélène Hegaret. "Effect of the antioxidant N-acetylcysteine on the depuration of the amnesic shellfish poisoning toxin, domoic acid, in the digestive gland of the king scallop Pecten maximus". Aquatic Living Resources 36 (2023): 14. http://dx.doi.org/10.1051/alr/2023011.

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Abstract (sommario):
Domoic acid (DA) is a potent neurotoxin produced by worldwide distributed diatoms of the genus Pseudo-nitzchia (PSN) and is responsible for Amnesic Shellfish Poisoning (ASP) in humans. King scallop Pecten maximus, a bivalve species of high commercial interest, is regularly subjected to blooms of Pseudo-nitzschia sp., thus accumulating and retaining high levels of DA for extended periods, leading to prolonged fisheries and aquaculture closures and important economic losses following increasingly recurrent toxic PSN blooms. The underlying mechanisms behind this accumulation and long toxin retention remain poorly understood so far. Fishermen and the aquaculture industry ask for methods to accelerate DA depuration in contaminated scallops, which has led to investigate the effect of some substances such as the antioxidant N-Acetylcysteine (NAC), which was previously found to improve up to four-fold DA depuration in P. maximus adductor muscle. Our study investigated the potential of NAC to accelerate DA depuration in all scallop tissues, including the digestive gland (DG), where most of the toxin is accumulated. Twenty-four contaminated adult scallops were collected following a toxic P. australis bloom in the Bay of Brest (France) and half were treated with the antioxidant NAC (250 mg L−1) for 6 days. HPLC toxin quantification analyses did not revealed any significant differences in the DA burdens in the DG between treated scallops and the control group. DA amounts in the adductor muscle and gonads were below the HPLC detection limit in both groups. Our results revealed that NAC does not thus appear as a commercially suitable solution for fisheries and aquaculture industries as DA depuration enhancer in the tested conditions.
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31

Baylón, Maribel, David U. Hernández-Becerril, Aldo Indacochea e Sara Purca. "Variabilidad espacio-temporal del fitoplancton de la ensenada Mackellar, Bahía Almirantazgo, Isla Rey Jorge, Antártida, durante el verano austral 2012/2013". Revista de Biología Marina y Oceanografía 54, n. 2 (13 settembre 2019): 151. http://dx.doi.org/10.22370/rbmo.2019.54.2.1809.

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Abstract (sommario):
El ecosistema antártico es una de las regiones del mundo más sensibles al cambio climático, y presenta amplia variabilidad ambiental que modifican los patrones espaciales y temporales de la estructura fitoplanctónica. Se estudió la comunidad del fitoplancton y su variabilidad espacial y temporal de la ensenada Mackellar-bahía Almirantazgo, Antártida, en los veranos australes de 2012 y 2013. Se hicieron muestreos de agua para fitoplancton y variables ambientales en 11 estaciones a tres profundidades (0, 10 y 20 m). La masa de Agua Superficial Antártica de la zona de estudio fue reconocida. La composición total del fitoplancton en la columna de agua incluyó 40 taxones de diatomeas, 6 taxones de dinoflagelados y varias especies de nanoflagelados (< 20 µm). Los nanoflagelados pertenecen a Prasinophyceae, Cryptophyta y Haptophyta. La alternancia entre diatomeas del microplancton (20-200 µm) y nanoflagelados (< 20 µm) fue evidente durante el período de estudio: en el verano 2012 el grupo de los nanoflagelados fue más abundante (86%), con especies de diatomeas del género Thalassiosira sp. (11%), y para el verano 2013 los nanoflagelados disminuyeron considerablemente (20%), la abundancia del fitoplancton estuvo dominada por diatomeas como Thalassiosira delicatula (36%), Pseudo-nitzschia grupo delicatissima (9%), Porosira glacialis (6%), y el dinoflagelado Gymnodinium sp. (19%). La densidad celular en el verano 2012 fue menor, en comparación con el verano 2013, donde ocurrió una floración de diatomeas. La diversidad del fitoplancton fue baja (< 1,97 bits⋅célula-1) en ambos veranos. Las principales diferencias fueron la dominancia de diatomeas del microplancton y nanoflagelados, las cuales probablemente fueron causadas por la disminución de la salinidad y el aumento de oxígeno superficial observado en la Antártida, producto del deshielo ocurrido durante los veranos 2012 y 2013.
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32

Lefebvre, Alain, e Camille Dezécache. "Trajectories of Changes in Phytoplankton Biomass, Phaeocystis globosa and Diatom (incl. Pseudo-nitzschia sp.) Abundances Related to Nutrient Pressures in the Eastern English Channel, Southern North Sea". Journal of Marine Science and Engineering 8, n. 6 (2 giugno 2020): 401. http://dx.doi.org/10.3390/jmse8060401.

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Abstract (sommario):
The phytoplankton compartment is particularly reactive to changes in nutrient concentration and is used as a quality indicator. Using a simple numerical approach, the response of emblematic harmful taxa from the eastern English Channel and southern North Sea to changes in nutrient inputs was studied. The method is based on a diachronic approach using averaged maxima over sliding periods of six years (1994–2018). This gave a final dataset containing pairs of points (number of years) for explained and explanatory variables. The temporal trajectory of the relationship between each pair of variables was then highlighted. Changes were represented as long-term trajectories that allowed a comparison to a reference/average situation. In addition, the relevance of the use of Phaeocystis globosa and the Pseudo-nitzchia complex as eutrophication species indicators was tested. Results showed a significant shift in the 2000s and different trajectories between diatoms and P. globosa abundances in response to changes in Dissolved Inorganic Nitrogen (DIN). The contrasting ecosystems under study reacted differently depending on the initial pressure. While a return to good ecological status does not seem feasible in the short term, it seems that these ecosystems were in an unstable intermediate state requiring continued efforts to reduce nutrient inputs.
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33

Niu, Biao‐Biao, Qi‐Xiang Zheng, Yang Liu, Nina Lundholm, Sing Tung Teng, Xu‐Dan Lu, Rui‐Wei Ran, Li Zhang e Yang Li. "Morphology, molecular phylogeny and biogeography revealed two new Pseudo‐nitzschia (Bacillariophyceae) species in Chinese waters". Journal of Systematics and Evolution, 6 settembre 2023. http://dx.doi.org/10.1111/jse.13016.

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Abstract (sommario):
AbstractPseudo‐nitzschia is a group of widely distributed planktonic diatoms. Some species produce the neurotoxin domoic acid. Herein, two new Pseudo‐nitzschia species were described from Chinese coastal waters and the South China Sea after combining morphological and molecular data, together with biogeographical traits. Pseudo‐nitzschia punctionis sp. nov. was similar to Pseudo‐nitzschia bipertita morphologically, but differed in poroid structure, which was undivided in P. punctionis but divided in two sectors in P. bipertita. This difference corresponded to the presence of two hemicompensatory base changes (HCBCs) in the secondary structure of internal transcribed spacer 2. Pseudo‐nitzschia polymorpha sp. nov. was sister to Pseudo‐nitzschia limii phylogenetically, but distinct by the various shapes of perforations on the valve and copula, which was supported by four HCBCs. In a metabarcoding analysis, multiple new ribotypes were identified within the two new species, and intraspecific genetic divergences were analyzed. Metabarcoding data revealed that P. punctionis had a broader temperature range (12.9–30.5 °C) than P. polymorpha (22.3–30.5 °C). Within the two new species, different traits were found among the amplicon sequence variants regarding temperature and biogeography, representing different microevolutionary directions under environmental selection. The two new species had different biogeographical traits when compared to their closely related species. Domoic acid was detected in strains of P. punctionis at a concentration of 13.5–17.7 fg/cell, but the toxin was not found in strains of P. polymorpha. A combination of characters based on laboratory strains and field metabarcoding data provided more data for delimiting Pseudo‐nitzschia species and gave new insights into their diversity and biogeography.
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34

He, Ziyan, Yang Chen, Yichao Wang, Kuiyan Liu, Qing Xu, Yang Li e Nansheng Chen. "Comparative Analysis of Pseudo-nitzschia Chloroplast Genomes Revealed Extensive Inverted Region Variation and Pseudo-nitzschia Speciation". Frontiers in Marine Science 9 (18 maggio 2022). http://dx.doi.org/10.3389/fmars.2022.784579.

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Abstract (sommario):
Pseudo-nitzschia is a species-rich genus where many species can induce harmful algae blooms (HABs) associated with the toxin domoic acid (DA) production. Despite the importance of Pseudo-nitzschia species to coastal environments, their genomic information is rather limited, hindering research on biodiversity and evolutionary analysis. In this study, we report full-length chloroplast genomes (cpDNAs) of nine Pseudo‐nitzschia, among which cpDNAs of eight Pseudo-nitzschia species were reported for the first time. The sizes of these Pseudo-nitzschia cpDNAs, which showed typical quadripartite structures, varied substantially, ranging from 116,546 bp to 158,840 bp in size. Comparative analysis revealed the loss of photosynthesis-related gene psaE in cpDNAs of all Pseudo-nitzschia species except that of P. americana, and the selective loss of rpl36 in P. hainanensis. Phylogenetic analysis showed that all Pseudo-nitzschia strains were grouped into two clades, with clade 1 containing cpDNAs of P. multiseries, P. pungens, P. multistriata, and P. americana, and clade 2 containing cpDNAs of P. hainanensis, P. cuspidata, Pseudo-nitzschia sp. CNS00097, P. delicatissima, and P. micropora. The small size of the P. americana cpDNA was primarily due to its shortened inverted repeat (IR) regions. While psaA and psaB were found in the IR regions of cpDNAs of other eight species, these two genes were found outside of the IR regions of P. americana cpDNA. In contrast, P. hainanensis had the largest size because of expansion of IR regions with each IR region containing 15 protein-coding genes (PCGs). Eleven genetic regions of these Pseudo-nitzschia cpDNAs exhibited high nucleotide diversity (Pi) values, suggesting that these regions may be used as molecular markers for distinguishing different Pseudo-nitzschia species with high resolution and high specificity. Phylogenetic analysis of the divergence of nine Pseudo-nitzschia species indicated that these species appeared at approximately 41 Mya. This study provides critical cpDNA resources for future research on the biodiversity and speciation of Pseudo-nitzschia species.
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35

Cicily, Lathika, K. Padmakumar, C. Asha Devi e V. Sanjeevan. "Occurrence of a multi-species diatom bloom dominated by Proboscia alata (Brightwell) Sandstorm along the southwest coast of India". Oceanological and Hydrobiological Studies 42, n. 1 (1 gennaio 2013). http://dx.doi.org/10.2478/s13545-013-0055-1.

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Abstract (sommario):
AbstractThe present communication reports on the occurrence of a multi-species diatom bloom in the upwelled waters along the southwest coast of India. During the late summer monsoon season (September 2009) a multi-species diatom bloom with a pale green discoloration of the sea surface was observed in the coastal waters of southwest coast of India. The bloom spread over an area of approximately 15 km2 along the coastal waters off Kannur (Lat. 11°59.471 N, Long. 75°03.446 E). Total diatom cell density of the bloom area was 16 × 104 cells l−1. Proboscia (=Rhizosolenia) alata (Brightwell) Sandstrom constituted 90% of the total phytoplankton population. Other phytoplankton groups that contributed to the bloom population included Chaetoceros spp., Pseudo-nitzschia spp., Rhizosolenia spp., Coscinodiscus sp., Leptocylindrus danicus, Thalassiosira sp., and Bacteriosira sp. Among these Pseudo-nitzschia multiseries, a toxic species with the ability to produce potent neurotoxin domoic acid, was observed with a cell density of 4 × 103 cells l−1. Surface chlorophyll a concentration of the bloom region was 14.1 μg l−1. Nutrient concentrations of the bloom area were 0.01 μmol l−1 for NO2-N, 0.1 μmol l−1 for NO3-N, 0.83 μmol l−1 for PO4-P and 11.44 μmol l−1 for SiO4.
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36

Hong, Zhuan, Qiliang Lai, Qiaoqi Luo, Simeng Jiang, Ruilin Zhu, Junrong Liang e Yahui Gao. "Erratum: Sulfitobacter pseudonitzschiae sp. nov., isolated from the toxic marine diatom Pseudo-nitzschia multiseries". International Journal of Systematic and Evolutionary Microbiology 72, n. 5 (26 maggio 2022). http://dx.doi.org/10.1099/ijsem.0.005403.

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37

FERREIRA, Leandro Cabanez, Maria Da G. G. Da SILVA CUNHA, Alessandra Lee B. FIRMO, Gislayne Cristina Palmeira BORGES, Jucicleide Cabral De LIMA, Emersom De Paula LIMA e Nayana Burque Antão Da SILVA. "Fitoplâncton como ferramenta de gestão ambiental na praia de Brasília Teimosa, Pernambuco, nordeste do Brasil." Tropical Oceanography 41, n. 1-2 (30 giugno 2013). http://dx.doi.org/10.5914/tropocean.v41i1-2.5567.

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Abstract (sommario):
O objetivo deste trabalho foi avaliar os parâmetros ambientais, biomassa, comunidade fitoplanctônica e espécies bioindicadoras, como ferramenta de gestão ambiental na praia Brasília Teimosa, Pernambuco (8º04’S; 34º52’W). Parâmetros abióticos (precipitação, temperatura da água, salinidade, material em suspensão, teor e taxa de saturação do oxigênio, nitrito, nitrato, fosfato e silicato) foram obtidos simultaneamente às coletas de fitoplâncton (garrafa e rede) no período chuvoso (maio; junho e julho/2005) e de estiagem (novembro e dezembro/2005; janeiro/2006). Com exceção dos valores de nitrato (maiores no período chuvoso), os demais parâmetros estiveram dentro dos padrões recomendados. Foram identificados 84 taxa, com maior representatividade das diatomáceas em abundância e frequência, Asterionellopsis glacialis, Chaetoceros lorenzianus, Cylindrotheca closterium, Helicotheca tamesis, Licmophora abbreviata e Skeletonema costatum. Altos níveis de nitrato, clorofila a, número de células por litro e espécies bioindicadores de eutrofização (Oscillatoria sp., Scenedesmus sp., Euglena sp., Synedra sp., Pleurosigma sp., Pseudo-nitzschia sp. e Skeletonema costatum) corroboraram a influência das atividades humanas na área, evidenciando um possível comprometimento da sanidade ambiental relacionado ao período de estiagem (período de maior exploração turística-recreativa). Palavras-chave: Bioindicadores, Clorofila a, Florescimentos, Diatomáceas.
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38

Percopo, Isabella, Maria Valeria Ruggiero, Diana Sarno, Lorenzo Longobardi, Rachele Rossi, Roberta Piredda e Adriana Zingone. "Phenological segregation suggests speciation by time in the planktonic diatom Pseudo‐nitzschia allochrona sp. nov." Ecology and Evolution 12, n. 8 (agosto 2022). http://dx.doi.org/10.1002/ece3.9155.

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39

Zhang, Shuwen, Tingting Zheng, Muyao Zhou, Biaobiao Niu e Yang Li. "Exposure to the mixotrophic dinoflagellate Lepidodinium sp. and its cues increase toxin production of Pseudo-nitzschia multiseries". Science of The Total Environment, gennaio 2024, 169812. http://dx.doi.org/10.1016/j.scitotenv.2023.169812.

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40

Lundholm, Nina, Anneliese L. Christensen, Anna K. J. Olesen, Bánk Beszteri, Sarah Lena Eggers, Bernd Krock e Andreas Altenburger. "Diversity, toxicity, and distribution of potentially toxic diatoms in Antarctic waters – with description of Pseudo-nitzschia meridionalis sp. nov. and P. glacialis sp. nov." Harmful Algae, settembre 2024, 102724. http://dx.doi.org/10.1016/j.hal.2024.102724.

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41

"Three novel species in the Pseudo-nitzschia pseudodelicatissima complex: P . batesiana sp. nov., P. lundholmiae sp. nov., and P. fukuyoi sp. nov. (Bacillariophyceae) from the Strait of Malacca, Malaysia (902-916)". Journal of Phycology 50, n. 1 (16 dicembre 2013): 227. http://dx.doi.org/10.1111/jpy.12151.

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42

Rachman, Arief. "Checklist and estimation of total number of phytoplankton species in Pari, Tidung, and Payung Islands, Indonesia". Biodiversitas Journal of Biological Diversity 21, n. 6 (13 maggio 2020). http://dx.doi.org/10.13057/biodiv/d210616.

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Abstract (sommario):
Abstract. Rachman A. 2020. Checklist and estimation of total number of phytoplankton species in Pari, Tidung, and Payung Islands, Indonesia. Biodiversitas 21: 2446-2454. A checklist of phytoplankton species from an anthropogenically pressured ecosystem has become a necessity to study the marine ecosystem changes and to detect the presence of introduced species in the area. This study aimed to list and describe the phytoplankton community in the Tidung, Pari, and Payung Islands and to estimate the total number of phytoplankton species in those islands. Sampling was conducted in May 2019 in 10 sites, consisted of 5 sites around Pari Island, 1 site next to Payung Island, and 4 sites around Tidung Island. As many as 150 species of phytoplankton, consisted of 109 species of diatoms, 38 species of dinoflagellates, 3 species of cyanobacteria, were recorded in this study. However, up to 47 phytoplankton species were estimated to be missing from the current sampling effort. Three diatom species, Chaetoceros curvisetus, Chaetoceros distans, and Chaetoceros affinis were the most abundant and most common species in the studied areas. Dinoflagellates were found in low density and have limited distribution compared to diatoms. Tripos trichoceros, Tripos fusus, and Tripos macroceros were the three most common dinoflagellates in the studied islands. Several notable harmful species, such as Pseudo-nitzschia spp., Alexandrium sp., Tripos furca, Cochlodinium sp., Dinophysis miles, Prorocentrum lima, and Noctiluca scintillans, were present albeit with low cell density. However, concern on future blooms of those harmful species in the greater water regions (i.e. Seribu Island or Jakarta Bay) should be noted, as it might cause environmental damages, including a shift in species dominance and disappearance of some native phytoplankton species from the ecosystem in the future.
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43

Ribeiro, Catherine Gérikas, Adriana Lopes dos Santos, Nicole Trefault, Dominique Marie, Connie Lovejoy e Daniel Vaulot. "Arctic phytoplankton microdiversity across the marginal ice zone: Subspecies vulnerability to sea-ice loss". Elem Sci Anth 12, n. 1 (2024). http://dx.doi.org/10.1525/elementa.2023.00109.

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Abstract (sommario):
Seasonal phytoplankton blooms are important Arctic phenomena, contributing to global primary production and biogeochemical cycling. The decline in sea-ice extent and thickness favors a longer open-water period with impacts on phytoplankton dynamics. Arctic net productivity is influenced by microalgae living associated with sea ice, with distinct species thought to be favored by ice-covered and ice-free waters. In this study, we investigated the phytoplankton community structure in Baffin Bay, a semi-enclosed sea where Arctic and North Atlantic water masses interact. We compared communities from the ice-free Atlantic-influenced eastern, the marginal ice zone, and the ice-covered Arctic-influenced western Baffin Bay. The community was characterized using 18S rRNA high-throughput amplicon sequencing and flow cytometry cell counting, and compared to environmental data collected during the Green Edge campaign. We sampled 16 stations grouped by sectors according to sea-ice cover. In the sectors associated with sea ice, phytoplankton formed a highly diverse community of smaller taxa, which contrasted with a low-diversity community in ice-free sectors, dominated by larger centric diatoms and Phaeocystis pouchetii adapted to high light/low nutrient conditions. Several phytoplankton species were flagged as indicators for the under-ice and marginal ice zone sectors, including ice-associated taxa such as the diatoms Melosira arctica and Pseudo-nitzschia seriata, but also subspecies representatives of the early-blooming alga Micromonas polaris and the cryptophyte Baffinella frigidus. The strong association of certain taxa with under-ice and marginal ice zone sectors, including Pterosperma sp., Chrysochromulina sp., Micromonas polaris, and B. frigidus, suggest that they might be indicators of diversity loss due to ongoing sea-ice changes in Baffin Bay. We report new intra-species variability of Micromonas polaris suggesting that seasonal specialists could wax and wane over the bloom and non-bloom periods, highlighting the need for detailed year-long studies and the importance of microdiversity when assessing the diversity and distribution of polar phytoplankton.
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44

Cheng, Jingguang, Justine Jacquin, Pascal Conan, Mireille Pujo-Pay, Valérie Barbe, Matthieu George, Pascale Fabre et al. "Relative Influence of Plastic Debris Size and Shape, Chemical Composition and Phytoplankton-Bacteria Interactions in Driving Seawater Plastisphere Abundance, Diversity and Activity". Frontiers in Microbiology 11 (13 gennaio 2021). http://dx.doi.org/10.3389/fmicb.2020.610231.

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Abstract (sommario):
The thin film of life that inhabits all plastics in the oceans, so-called “plastisphere,” has multiple effects on the fate and impacts of plastic in the marine environment. Here, we aimed to evaluate the relative influence of the plastic size, shape, chemical composition, and environmental changes such as a phytoplankton bloom in shaping the plastisphere abundance, diversity and activity. Polyethylene (PE) and polylactide acid (PLA) together with glass controls in the forms of meso-debris (18 mm diameter) and large-microplastics (LMP; 3 mm diameter), as well as small-microplastics (SMP) of 100 μm diameter with spherical or irregular shapes were immerged in seawater during 2 months. Results of bacterial abundance (confocal microscopy) and diversity (16S rRNA Illumina sequencing) indicated that the three classical biofilm colonization phases (primo-colonization after 3 days; growing phase after 10 days; maturation phase after 30 days) were not influenced by the size and the shape of the materials, even when a diatom bloom (Pseudo-nitzschia sp.) occurred after the first month of incubation. However, plastic size and shape had an effect on bacterial activity (3H leucine incorporation). Bacterial communities associated with the material of 100 μm size fraction showed the highest activity compared to all other material sizes. A mature biofilm developed within 30 days on all material types, with higher bacterial abundance on the plastics compared to glass, and distinct bacterial assemblages were detected on each material type. The diatom bloom event had a great impact on the plastisphere of all materials, resulting in a drastic change in diversity and activity. Our results showed that the plastic size and shape had relatively low influence on the plastisphere abundance, diversity, and activity, as compared to the plastic composition or the presence of a phytoplankton bloom.
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45

Jacquemot, Loïc, Jean-Éric Tremblay, Carlee Morency e Connie Lovejoy. "Stratification and summer protist communities in the Arctic influenced coastal systems of Nunavik (Québec, Canada)". Frontiers in Marine Science 11 (29 gennaio 2024). http://dx.doi.org/10.3389/fmars.2024.1321604.

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Abstract (sommario):
Phytoplankton and other protists in the 3 µm to 50 µm size fraction are grazed on by zooplankton and form the base of Arctic marine food webs essential for local indigenous communities. Anthropogenic climate change is increasing stratification over much of the Arctic Ocean and surrounding seas, but the influence of stratification on protist communities in more coastal regions along Eastern Hudson Bay, Hudson Strait and Ungava Bay is little known. We used 18S rRNA and rDNA amplicon sequencing during two consecutive summers (2017 and 2018) and detailed water column properties to compare the 3 µm to 50 µm protist communities under contrasting stratification regimes in the Eastern Hudson Bay Complex. We found that the surface mixed layer in Eastern Hudson Bay, which is under the influence of river runoff, was strongly stratified and dominated by mixotrophic and bacterivorous taxa, mostly the dinoflagellates Heterocapsa rotundata and Gymnodiniales spp., and a more diatom-dominated community at the Subsurface Chlorophyll Maximum (SCM), which persisted in deeper colder and more saline water. The massive sequencing effort retrieved seven putative toxic algae from the upper warmer waters of eastern Hudson Bay. These included Pseudo-nitzschia spp. and potentially harmful dinoflagellates, most notably Alexandrium sp. The persistent weaker stratification conditions in Hudson Strait and Ungava Bay in summer favored a different diatom community, dominated by Chaetoceros spp. and Thalassiosira spp., and small photosynthetic flagellates including Phaeocystis pouchetii and Micromonas polaris. As freshwater input increases and stratification intensifies in the Arctic, our findings suggest the summer dinoflagellate-based community seen in coastal Hudson Bay may also be favored in other regions receiving increased river runoff. These conditions could also favor harmful algal events. The Hudson Strait and Ungava Bay protist communities were found nearer the surface and consisted of diverse species able to profit from ongoing nutrient input due to tidal mixing. These results suggest greater resilience in this and other tidally influenced coastal Arctic Bays lacking larger rivers inputs.
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